Lag0040443 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0040443 vs. NCBI nr
Match: XP_020413966.1 (metalloendoproteinase 2-MMP isoform X2 [Prunus persica])

HSP 1 Score: 254.6 bits (649), Expect = 1.0e-63
Identity = 140/301 (46.51%), Postives = 183/301 (60.80%), Query Frame = 0

Query: 18  ILFLSCAVQSAAAAAAEQNNAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSL---KQ 77
           ++F+  +  + + +  +Q++ F FI+HL G  KG+ V G+ +LKKY+ +FGYL+    K 
Sbjct: 46  LIFVLLSQNTHSKSTHDQHDPFKFIQHLEGCHKGQNVSGLQELKKYLTKFGYLNYDHSKH 105

Query: 78  QENDLFDDALESAIKGYQLNFHMKSTGKMDDDTVKQMMIPRCGIADRALSDQKSRQ---- 137
             ++ FDD LESAIK YQ ++H+K TG +D  T KQMM+PRCG+ D     +K  +    
Sbjct: 106 ANDEEFDDILESAIKSYQKSYHLKVTGTLDTTTGKQMMMPRCGVPDVVNGTRKGSKKHNH 165

Query: 138 -----------------RPFSKTDLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDFT 197
                            R +SKT LTY+F+S      G  N+RSI  QAFQ WA VT FT
Sbjct: 166 KHKSIHGVAHYEFFPGPRRWSKTHLTYRFSSSVNQVPGTQNVRSICAQAFQRWAQVTSFT 225

Query: 198 FEEVPENSPADIEIKFGSGRHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKWSTN 257
           FEEVP  S ADI I F  G HGD S FDG  G F H   P  G      H DADE+WS+N
Sbjct: 226 FEEVPATSAADITIGFHRGNHGDGSAFDGPRGTFAHANPPRGG----NFHYDADERWSSN 285

Query: 258 LG---MDSESIAIHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALYG 292
            G   +D ES+A+HEIGHLLGL+HN + PDAIMYP  + G +KR+LH+DDI  +  ALYG
Sbjct: 286 PGPNEVDLESVAVHEIGHLLGLNHNPDLPDAIMYPYFNYGSIKRNLHRDDIDGIR-ALYG 341

BLAST of Lag0040443 vs. NCBI nr
Match: XP_020413072.1 (metalloendoproteinase 5-MMP [Prunus persica])

HSP 1 Score: 254.2 bits (648), Expect = 1.3e-63
Identity = 140/302 (46.36%), Postives = 183/302 (60.60%), Query Frame = 0

Query: 18  ILFLSCAVQSAAAAAAEQNNAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSL---KQ 77
           ++F+  +  + + +  +Q++ F FI+HL G  KG+ V G+ +LKKY+ +FGYL+    K 
Sbjct: 84  LIFVLLSQNTHSKSTHDQHDPFKFIQHLEGCHKGQNVSGLQELKKYLTKFGYLNYDHSKH 143

Query: 78  QENDLFDDALESAIKGYQLNFHMKSTGKMDDDTVKQMMIPRCGIADRALSDQKSRQ---- 137
             ++ FDD LESAIK YQ ++H+K TG +D  T KQMM+PRCG+ D     +K  +    
Sbjct: 144 ANDEEFDDILESAIKSYQKSYHLKVTGTLDTTTGKQMMMPRCGVPDVVNGTRKGSKKHNH 203

Query: 138 ------------------RPFSKTDLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDF 197
                             R +SKT LTY+F+S      G  N+RSI  QAFQ WA VT F
Sbjct: 204 KHKSIHGVAHYEFFFPGPRRWSKTHLTYRFSSSVNQVPGTQNVRSICAQAFQRWAQVTSF 263

Query: 198 TFEEVPENSPADIEIKFGSGRHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKWST 257
           TFEEVP  S ADI I F  G HGD S FDG  G F H   P  G      H DADE+WS+
Sbjct: 264 TFEEVPATSAADITIGFHRGNHGDGSAFDGPRGTFAHANPPRGG----NFHYDADERWSS 323

Query: 258 NLG---MDSESIAIHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALY 292
           N G   +D ES+A+HEIGHLLGL+HN + PDAIMYP  + G +KR+LH+DDI  +  ALY
Sbjct: 324 NPGPNEVDLESVAVHEIGHLLGLNHNPDLPDAIMYPYFNYGSIKRNLHRDDIDGIR-ALY 380

BLAST of Lag0040443 vs. NCBI nr
Match: ONI23727.1 (hypothetical protein PRUPE_2G204500 [Prunus persica])

HSP 1 Score: 254.2 bits (648), Expect = 1.3e-63
Identity = 140/302 (46.36%), Postives = 183/302 (60.60%), Query Frame = 0

Query: 18  ILFLSCAVQSAAAAAAEQNNAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSL---KQ 77
           ++F+  +  + + +  +Q++ F FI+HL G  KG+ V G+ +LKKY+ +FGYL+    K 
Sbjct: 17  LIFVLLSQNTHSKSTHDQHDPFKFIQHLEGCHKGQNVSGLQELKKYLTKFGYLNYDHSKH 76

Query: 78  QENDLFDDALESAIKGYQLNFHMKSTGKMDDDTVKQMMIPRCGIADRALSDQKSRQ---- 137
             ++ FDD LESAIK YQ ++H+K TG +D  T KQMM+PRCG+ D     +K  +    
Sbjct: 77  ANDEEFDDILESAIKSYQKSYHLKVTGTLDTTTGKQMMMPRCGVPDVVNGTRKGSKKHNH 136

Query: 138 ------------------RPFSKTDLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDF 197
                             R +SKT LTY+F+S      G  N+RSI  QAFQ WA VT F
Sbjct: 137 KHKSIHGVAHYEFFFPGPRRWSKTHLTYRFSSSVNQVPGTQNVRSICAQAFQRWAQVTSF 196

Query: 198 TFEEVPENSPADIEIKFGSGRHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKWST 257
           TFEEVP  S ADI I F  G HGD S FDG  G F H   P  G      H DADE+WS+
Sbjct: 197 TFEEVPATSAADITIGFHRGNHGDGSAFDGPRGTFAHANPPRGG----NFHYDADERWSS 256

Query: 258 NLG---MDSESIAIHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALY 292
           N G   +D ES+A+HEIGHLLGL+HN + PDAIMYP  + G +KR+LH+DDI  +  ALY
Sbjct: 257 NPGPNEVDLESVAVHEIGHLLGLNHNPDLPDAIMYPYFNYGSIKRNLHRDDIDGIR-ALY 313

BLAST of Lag0040443 vs. NCBI nr
Match: XP_008376281.3 (metalloendoproteinase 2-MMP-like [Malus domestica])

HSP 1 Score: 252.3 bits (643), Expect = 5.1e-63
Identity = 141/287 (49.13%), Postives = 172/287 (59.93%), Query Frame = 0

Query: 35  QNNAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSL---KQQENDLFDDALESAIKGY 94
           Q+++F FI+HL G  KG+ V G+ +LKKY  +FGYL+    K   +D FDD LESAIK Y
Sbjct: 38  QHDSFKFIQHLEGCHKGQNVSGLQELKKYFTKFGYLNYDHSKHANDDEFDDLLESAIKSY 97

Query: 95  QLNFHMKSTGKMDDDTVKQMMIPRCGIADRALSDQKSRQR-------------------- 154
           Q +FH+  TG +D  TVK+MM+PRCG+ D     +K  ++                    
Sbjct: 98  QKSFHLNVTGSLDTTTVKRMMVPRCGVPDVVNGTRKGTKKHNRKLKSIHGVSHYEFFFPG 157

Query: 155 ----PFSKTDLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDFTFEEVPENSPADIEI 214
               P +KT L Y+F S A    G  N+RSI  QAFQ WA VT FTFEEVP  S ADI I
Sbjct: 158 PLRWPPTKTHLRYRFASSASQVPGTENVRSICAQAFQRWAQVTSFTFEEVPATSSADITI 217

Query: 215 KFGSGRHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKWSTNLG---MDSESIAIH 274
            F  G HGD +PFDG  GV  H   P TG      H DADEKWS N G   +D ES+A+H
Sbjct: 218 GFHRGAHGDTAPFDGFKGVLAHA-NPPTGG---NFHFDADEKWSANPGPDELDLESVAVH 277

Query: 275 EIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALYGL 292
           EIGHLLGLDH+ + PDAIMYP    G+VKR L +DDI  +  ALYGL
Sbjct: 278 EIGHLLGLDHDLDLPDAIMYPEFSYGIVKRDLTRDDIDGIR-ALYGL 319

BLAST of Lag0040443 vs. NCBI nr
Match: CAB4270434.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 250.4 bits (638), Expect = 1.9e-62
Identity = 138/302 (45.70%), Postives = 180/302 (59.60%), Query Frame = 0

Query: 18  ILFLSCAVQSAAAAAAEQNNAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSL---KQ 77
           ++F+     S + +  +Q++ F FI+HL G  KG+ V G+ +LKKY+ +FGYL+    K 
Sbjct: 46  LIFVLLPQNSHSKSTHDQHDPFKFIQHLEGCHKGQNVSGLQELKKYLTKFGYLNYDHSKH 105

Query: 78  QENDLFDDALESAIKGYQLNFHMKSTGKMDDDTVKQMMIPRCGIADRALSDQKSRQ---- 137
             +D FDD LESAIK YQ ++H++ TG +D  T KQMM+PRCG+ D     +K  +    
Sbjct: 106 ANDDEFDDILESAIKSYQKSYHLEVTGTLDTSTGKQMMMPRCGVPDVVNGTRKGSKKHNH 165

Query: 138 ------------------RPFSKTDLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDF 197
                             R +SKT LTY+F+S      G  N+RSI  QAFQ WA VT F
Sbjct: 166 KHKSIHGVTHYEFFFPGPRRWSKTHLTYRFSSSVNQVPGTQNVRSICAQAFQRWAQVTSF 225

Query: 198 TFEEVPENSPADIEIKFGSGRHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKWST 257
           TFEEVP  + ADI I F  G HGD S FDG  G F H   P  G      H DADE+WS+
Sbjct: 226 TFEEVPATTAADITIGFHRGNHGDGSAFDGPRGTFAHANPPRGG----NFHYDADERWSS 285

Query: 258 NLG---MDSESIAIHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALY 292
           N G   +D ES+ +HEIGH LGL+HN + PDAIMYP  D G++K+ LH+DDI  +  ALY
Sbjct: 286 NPGPNEVDLESVTVHEIGHPLGLNHNPDLPDAIMYPYFDHGIIKQDLHRDDIDGIR-ALY 342

BLAST of Lag0040443 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 5.5e-44
Identity = 113/288 (39.24%), Postives = 147/288 (51.04%), Query Frame = 0

Query: 37  NAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSLKQQENDLFDDALESAIKGYQLNFH 96
           NA+     LAG   G  + G++KLK+Y + FGY++      D FDD L+SAI  YQ NF+
Sbjct: 40  NAWETFSKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDDFDDVLQSAINTYQKNFN 99

Query: 97  MKSTGKMDDDTVKQMMIPRCGIADRALSDQKSRQR--------------------PFSKT 156
           +K TGK+D  T++Q++ PRCG  D  L D  S                       P  K 
Sbjct: 100 LKVTGKLDSSTLRQIVKPRCGNPD--LIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKR 159

Query: 157 DLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDFTFEEVPENSPADIEIKFGSGRHGD 216
           DLTY F     ++     ++ +F +AF  WA+VT   F        ADI I F SG HGD
Sbjct: 160 DLTYAFAPQNNLT---DEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGD 219

Query: 217 NSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKWSTNLG------------MDSESIAIH 276
             PFDGA G   H   P TG     +HLD DE W  + G            +D ES+A+H
Sbjct: 220 GEPFDGAMGTLAHASSPPTGM----LHLDGDEDWLISNGEISRRILPVTTVVDLESVAVH 279

Query: 277 EIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALYGLN 293
           EIGHLLGL H++   DAIM+P+I  G  K  L +DDI  +   LYG N
Sbjct: 280 EIGHLLGLGHSSVE-DAIMFPAISGGDRKVELAKDDIEGIQ-HLYGGN 316

BLAST of Lag0040443 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.5e-41
Identity = 106/294 (36.05%), Postives = 162/294 (55.10%), Query Frame = 0

Query: 24  AVQSAAAAAA---EQNNAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSLKQQ-ENDL 83
           A QS A A       N+ +H    L   + G +V G+++LK+Y+  FGY++   +  +D+
Sbjct: 34  AEQSTAKATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSELKRYLHRFGYVNDGSEIFSDV 93

Query: 84  FDDALESAIKGYQLNFHMKSTGKMDDDTVKQMMIPRCGIADRALSDQK------------ 143
           FD  LESAI  YQ N  +  TG++D  TV  M +PRCG++D  ++               
Sbjct: 94  FDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSDTHMTINNDFLHTTAHYTYF 153

Query: 144 SRQRPFSKTDLTYKFN-SHALISIGEINIRSIFGQAFQIWADVTDFTFEEVPENSPADIE 203
           + +  +++  LTY  + +H L  +   +++++F +AF  W+ V   +FEEV + + AD++
Sbjct: 154 NGKPKWNRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLK 213

Query: 204 IKFGSGRHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKW--------STNLGMDS 263
           I F +G HGD  PFDG  G   H + PE GR    +HLDA E W        S+ + +D 
Sbjct: 214 IGFYAGDHGDGLPFDGVLGTLAHAFAPENGR----LHLDAAETWIVDDDLKGSSEVAVDL 273

Query: 264 ESIAIHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALYGLN 293
           ES+A HEIGHLLGL H+++   A+MYPS+     K  L  DD++ V   LYG N
Sbjct: 274 ESVATHEIGHLLGLGHSSQE-SAVMYPSLRPRTKKVDLTVDDVAGV-LKLYGPN 321

BLAST of Lag0040443 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 169.9 bits (429), Expect = 4.4e-41
Identity = 101/266 (37.97%), Postives = 138/266 (51.88%), Query Frame = 0

Query: 51  GRYVRGITKLKKYMKEFGYLSLKQQENDLFDDALESAIKGYQLNFHMKSTGKMDDDTVKQ 110
           G+  +G++ +K Y    GY+      +D FDD L SAIK YQ N+++  TGK D +T+KQ
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPHFDDNFDDTLVSAIKTYQKNYNLNVTGKFDINTLKQ 107

Query: 111 MMIPRCGIADRALSDQKS------------RQRP---FSKTDLTYKFNSHALISIGEINI 170
           +M PRCG+ D  ++  K+            +  P      T LTY F+    +   +   
Sbjct: 108 IMTPRCGVPDIIINTNKTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRL---DDTF 167

Query: 171 RSIFGQAFQIWADVTDFTFEEVPENSPADIEIKFGSGRHGDNSPFDGAGGVFGHVWRPET 230
           +S   +AF  W  V +  F+E      A+I+I F S  HGD  PFDG GG+ GH + P  
Sbjct: 168 KSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTD 227

Query: 231 GRKPLEIHLDADEKW---------STNLGMDSESIAIHEIGHLLGLDHNTENPDAIMYPS 290
           GR     H DADE W               D ES+A+HEIGHLLGL H+++   AIMYPS
Sbjct: 228 GR----CHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSD-LRAIMYPS 287

Query: 291 IDSGMVKRHLHQDDISAVHAALYGLN 293
           I     K +L QDDI  +   LYG+N
Sbjct: 288 IPPRTRKVNLAQDDIDGIR-KLYGIN 304

BLAST of Lag0040443 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 4.5e-38
Identity = 101/290 (34.83%), Postives = 149/290 (51.38%), Query Frame = 0

Query: 44  HLAGSRKGRYVRGITKLKKYMKEFGYL--SLKQQENDLFDDALESAIKGYQLNFHMKSTG 103
           +  G   G+ V G+ ++KKY + FGY+  +      D FDD L++A++ YQ NF++  TG
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTG 105

Query: 104 KMDDDTVKQMMIPRCGIADRALSD-------QKSRQRPFSKT------------------ 163
           ++D  T++ ++IPRCG  D            +K+ +  FS+T                  
Sbjct: 106 ELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWP 165

Query: 164 ----DLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDFTFEEVPENSPADIEIKFGSG 223
               DLTY F+    ++     ++S+F +AF  W+DVT   F      S +DI I F +G
Sbjct: 166 RNRRDLTYAFDPKNPLT---EEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTG 225

Query: 224 RHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKW----------STNLGMDSESIA 283
            HGD  PFDG  G   H + P +G+     HLDADE W          S    +D ES+A
Sbjct: 226 DHGDGEPFDGVLGTLAHAFSPPSGK----FHLDADENWVVSGDLDSFLSVTAAVDLESVA 285

Query: 284 IHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALYGLN 293
           +HEIGHLLGL H++   ++IMYP+I +G  K  L  DD+  +   LYG N
Sbjct: 286 VHEIGHLLGLGHSSVE-ESIMYPTITTGKRKVDLTNDDVEGIQ-YLYGAN 326

BLAST of Lag0040443 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 7.7e-38
Identity = 103/300 (34.33%), Postives = 147/300 (49.00%), Query Frame = 0

Query: 37  NAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYL---SLKQQENDLFDDALESAIKGYQL 96
           N ++   +  G   G+   G+  LK+Y + FGY+   +L     D FDD L++A++ YQ 
Sbjct: 41  NPWNSFLNFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQR 100

Query: 97  NFHMKSTGKMDDDTVKQMMIPRCGIAD--RALSDQKSRQRPF------------------ 156
           NF +  TG +D+ T+K ++IPRCG  D     S   S ++ F                  
Sbjct: 101 NFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYS 160

Query: 157 ----------SKTDLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDFTFEEVPENSPA 216
                     ++ DLTY F+    ++     ++S+F +AF  W +VT  TF  V   S +
Sbjct: 161 FFPGEPRWPRNRRDLTYAFDPRNALT---EEVKSVFSRAFTRWEEVTPLTFTRVERFSTS 220

Query: 217 DIEIKFGSGRHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKW-----------ST 276
           DI I F SG HGD  PFDG      H + P TG      HLD +E W           S 
Sbjct: 221 DISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTG----HFHLDGEENWIVSGEGGDGFISV 280

Query: 277 NLGMDSESIAIHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALYGLN 293
           +  +D ES+A+HEIGHLLGL H++    +IMYP+I +G  K  L  DD+  V   LYG N
Sbjct: 281 SEAVDLESVAVHEIGHLLGLGHSSVE-GSIMYPTIRTGRRKVDLTTDDVEGVQ-YLYGAN 331

BLAST of Lag0040443 vs. ExPASy TrEMBL
Match: A0A251QIR5 (ZnMc domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G204500 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 6.5e-64
Identity = 140/302 (46.36%), Postives = 183/302 (60.60%), Query Frame = 0

Query: 18  ILFLSCAVQSAAAAAAEQNNAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSL---KQ 77
           ++F+  +  + + +  +Q++ F FI+HL G  KG+ V G+ +LKKY+ +FGYL+    K 
Sbjct: 17  LIFVLLSQNTHSKSTHDQHDPFKFIQHLEGCHKGQNVSGLQELKKYLTKFGYLNYDHSKH 76

Query: 78  QENDLFDDALESAIKGYQLNFHMKSTGKMDDDTVKQMMIPRCGIADRALSDQKSRQ---- 137
             ++ FDD LESAIK YQ ++H+K TG +D  T KQMM+PRCG+ D     +K  +    
Sbjct: 77  ANDEEFDDILESAIKSYQKSYHLKVTGTLDTTTGKQMMMPRCGVPDVVNGTRKGSKKHNH 136

Query: 138 ------------------RPFSKTDLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDF 197
                             R +SKT LTY+F+S      G  N+RSI  QAFQ WA VT F
Sbjct: 137 KHKSIHGVAHYEFFFPGPRRWSKTHLTYRFSSSVNQVPGTQNVRSICAQAFQRWAQVTSF 196

Query: 198 TFEEVPENSPADIEIKFGSGRHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKWST 257
           TFEEVP  S ADI I F  G HGD S FDG  G F H   P  G      H DADE+WS+
Sbjct: 197 TFEEVPATSAADITIGFHRGNHGDGSAFDGPRGTFAHANPPRGG----NFHYDADERWSS 256

Query: 258 NLG---MDSESIAIHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALY 292
           N G   +D ES+A+HEIGHLLGL+HN + PDAIMYP  + G +KR+LH+DDI  +  ALY
Sbjct: 257 NPGPNEVDLESVAVHEIGHLLGLNHNPDLPDAIMYPYFNYGSIKRNLHRDDIDGIR-ALY 313

BLAST of Lag0040443 vs. ExPASy TrEMBL
Match: A0A6J5U4X3 (ZnMc domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS16549 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 9.4e-63
Identity = 138/302 (45.70%), Postives = 180/302 (59.60%), Query Frame = 0

Query: 18  ILFLSCAVQSAAAAAAEQNNAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSL---KQ 77
           ++F+     S + +  +Q++ F FI+HL G  KG+ V G+ +LKKY+ +FGYL+    K 
Sbjct: 46  LIFVLLPQNSHSKSTHDQHDPFKFIQHLEGCHKGQNVSGLQELKKYLTKFGYLNYDHSKH 105

Query: 78  QENDLFDDALESAIKGYQLNFHMKSTGKMDDDTVKQMMIPRCGIADRALSDQKSRQ---- 137
             +D FDD LESAIK YQ ++H++ TG +D  T KQMM+PRCG+ D     +K  +    
Sbjct: 106 ANDDEFDDILESAIKSYQKSYHLEVTGTLDTSTGKQMMMPRCGVPDVVNGTRKGSKKHNH 165

Query: 138 ------------------RPFSKTDLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDF 197
                             R +SKT LTY+F+S      G  N+RSI  QAFQ WA VT F
Sbjct: 166 KHKSIHGVTHYEFFFPGPRRWSKTHLTYRFSSSVNQVPGTQNVRSICAQAFQRWAQVTSF 225

Query: 198 TFEEVPENSPADIEIKFGSGRHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKWST 257
           TFEEVP  + ADI I F  G HGD S FDG  G F H   P  G      H DADE+WS+
Sbjct: 226 TFEEVPATTAADITIGFHRGNHGDGSAFDGPRGTFAHANPPRGG----NFHYDADERWSS 285

Query: 258 NLG---MDSESIAIHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALY 292
           N G   +D ES+ +HEIGH LGL+HN + PDAIMYP  D G++K+ LH+DDI  +  ALY
Sbjct: 286 NPGPNEVDLESVTVHEIGHPLGLNHNPDLPDAIMYPYFDHGIIKQDLHRDDIDGIR-ALY 342

BLAST of Lag0040443 vs. ExPASy TrEMBL
Match: A0A5N5G868 (Metalloendoproteinase 1-like OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_041989 PE=3 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 1.2e-62
Identity = 144/309 (46.60%), Postives = 177/309 (57.28%), Query Frame = 0

Query: 13  TLILSILFLSCAVQSAAAAAAEQNNAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSL 72
           +L++ +L     +         Q+++F FI+HL G  KG+ V G+ +LKKY+ +FGYL+ 
Sbjct: 13  SLLIFVLLSQQTLAKPTDPHGHQHDSFKFIQHLEGCHKGQNVSGLQELKKYLTKFGYLNY 72

Query: 73  ---KQQENDLFDDALESAIKGYQLNFHMKSTGKMDDDTVKQMMIPRCGIADRALSDQKSR 132
              K   +D FDD LESAIK YQ +FH+  TG +D  TVKQMM+PRCG+ D     +K  
Sbjct: 73  DHSKHANDDEFDDLLESAIKSYQKSFHLNVTGSLDTTTVKQMMVPRCGVPDVVNGTRKGT 132

Query: 133 QR------------------------PFSKTDLTYKFNSHALISIGEINIRSIFGQAFQI 192
           +                         P +KT L Y+F S A    G  N+RSI  QAFQ 
Sbjct: 133 KNHNRKLKSIHGVSYYEFFFPGPLRWPPTKTHLRYRFASSASQVPGTENVRSICAQAFQK 192

Query: 193 WADVTDFTFEEVPENSPADIEIKFGSGRHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLD 252
           WA VT FTFEEVP  S ADI I F  G HGD +PFDG  G   H   P TG      H D
Sbjct: 193 WAQVTSFTFEEVPATSSADITIGFHRGAHGDMAPFDGFKGALAHA-NPPTGG---NFHFD 252

Query: 253 ADEKWSTNLG---MDSESIAIHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDIS 292
           ADEKWS N G   +D ES+A+HEIGHLLGLDHN + PDAIMYP    G+VKR L  DDI 
Sbjct: 253 ADEKWSANPGPNEVDLESVAVHEIGHLLGLDHNLDLPDAIMYPKFSYGIVKRDLTGDDID 312

BLAST of Lag0040443 vs. ExPASy TrEMBL
Match: A0A5N5F5B8 (Metalloendoproteinase 1-like OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_000883 PE=3 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 1.2e-62
Identity = 144/309 (46.60%), Postives = 177/309 (57.28%), Query Frame = 0

Query: 13  TLILSILFLSCAVQSAAAAAAEQNNAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSL 72
           +L++ +L     +         Q+++F FI+HL G  KG+ V G+ +LKKY+ +FGYL+ 
Sbjct: 16  SLLIFVLLSQKTLAKPTDPHGHQHDSFKFIQHLEGCHKGQNVSGLQELKKYLTKFGYLNY 75

Query: 73  ---KQQENDLFDDALESAIKGYQLNFHMKSTGKMDDDTVKQMMIPRCGIADRALSDQKSR 132
              K   +D FDD LESAIK YQ +FH+  TG +D  TVKQMM+PRCG+ D     +K  
Sbjct: 76  DHSKHANDDEFDDLLESAIKSYQKSFHLNVTGSLDTTTVKQMMVPRCGVPDVVNGTRKGT 135

Query: 133 QR------------------------PFSKTDLTYKFNSHALISIGEINIRSIFGQAFQI 192
           +                         P +KT L Y+F S A    G  N+RSI  QAFQ 
Sbjct: 136 KNHNRKLKSIHGVSYYEFFFPGPLRWPPTKTHLRYRFASSASQVPGTENVRSICAQAFQK 195

Query: 193 WADVTDFTFEEVPENSPADIEIKFGSGRHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLD 252
           WA VT FTFEEVP  S ADI I F  G HGD +PFDG  G   H   P TG      H D
Sbjct: 196 WAQVTSFTFEEVPATSSADITIGFHRGAHGDMAPFDGFKGALAHA-NPPTGG---NFHFD 255

Query: 253 ADEKWSTNLG---MDSESIAIHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDIS 292
           ADEKWS N G   +D ES+A+HEIGHLLGLDHN + PDAIMYP    G+VKR L  DDI 
Sbjct: 256 ADEKWSANPGPNEVDLESVAVHEIGHLLGLDHNLDLPDAIMYPKFSYGIVKRDLTGDDID 315

BLAST of Lag0040443 vs. ExPASy TrEMBL
Match: A0A6J5WL88 (ZnMc domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS16129 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 2.7e-62
Identity = 140/304 (46.05%), Postives = 180/304 (59.21%), Query Frame = 0

Query: 18  ILFLSCAVQSAAAAAAEQNNAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSL---KQ 77
           ++F+     S + +  +Q++ F FI+HL G  KG+ V G+ +LKKY+ +FGYL+    K 
Sbjct: 17  LIFVLLPQNSHSKSTHDQHDPFKFIQHLEGCHKGQNVSGLQELKKYLTKFGYLNYDHSKH 76

Query: 78  QENDLFDDALESAIKGYQLNFHMKSTGKMDDDTVKQMMIPRCGIADRALSDQKSRQ---- 137
             +D FDD LESAIK YQ ++H+K TG +D  T KQMM+PRCG+ D     +K  +    
Sbjct: 77  ANDDEFDDILESAIKSYQKSYHLKVTGTLDTSTGKQMMMPRCGVPDVVNGTRKGSKKHNH 136

Query: 138 ------------------RPFSKTDLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDF 197
                             R +SKT LTY+F+S      G  N+RSI  QAFQ WA VT F
Sbjct: 137 KHKSIHGVAHYEFFFPGPRRWSKTHLTYRFSSSVNQVPGTQNVRSICAQAFQRWAQVTSF 196

Query: 198 TFEEVPENSPADIEIKFGSGRHGD--NSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKW 257
           TFEEVP  S ADI I F  G HGD   + FDG  G   H   P  G      H DADE+W
Sbjct: 197 TFEEVPATSAADITIGFHRGNHGDGPRNAFDGPRGTLAHANPPRGG----NFHYDADERW 256

Query: 258 STNLG---MDSESIAIHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAA 292
           S+N G   +D ES+ +HEIGHLLGL+HN + PDAIMYP  D G++KR LH+DDI  +  A
Sbjct: 257 SSNPGPNEVDLESVTVHEIGHLLGLNHNPDLPDAIMYPYFDHGIIKRDLHRDDIDGIR-A 315

BLAST of Lag0040443 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 179.5 bits (454), Expect = 3.9e-45
Identity = 113/288 (39.24%), Postives = 147/288 (51.04%), Query Frame = 0

Query: 37  NAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSLKQQENDLFDDALESAIKGYQLNFH 96
           NA+     LAG   G  + G++KLK+Y + FGY++      D FDD L+SAI  YQ NF+
Sbjct: 40  NAWETFSKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDDFDDVLQSAINTYQKNFN 99

Query: 97  MKSTGKMDDDTVKQMMIPRCGIADRALSDQKSRQR--------------------PFSKT 156
           +K TGK+D  T++Q++ PRCG  D  L D  S                       P  K 
Sbjct: 100 LKVTGKLDSSTLRQIVKPRCGNPD--LIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKR 159

Query: 157 DLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDFTFEEVPENSPADIEIKFGSGRHGD 216
           DLTY F     ++     ++ +F +AF  WA+VT   F        ADI I F SG HGD
Sbjct: 160 DLTYAFAPQNNLT---DEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGD 219

Query: 217 NSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKWSTNLG------------MDSESIAIH 276
             PFDGA G   H   P TG     +HLD DE W  + G            +D ES+A+H
Sbjct: 220 GEPFDGAMGTLAHASSPPTGM----LHLDGDEDWLISNGEISRRILPVTTVVDLESVAVH 279

Query: 277 EIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALYGLN 293
           EIGHLLGL H++   DAIM+P+I  G  K  L +DDI  +   LYG N
Sbjct: 280 EIGHLLGLGHSSVE-DAIMFPAISGGDRKVELAKDDIEGIQ-HLYGGN 316

BLAST of Lag0040443 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.1e-42
Identity = 106/294 (36.05%), Postives = 162/294 (55.10%), Query Frame = 0

Query: 24  AVQSAAAAAA---EQNNAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYLSLKQQ-ENDL 83
           A QS A A       N+ +H    L   + G +V G+++LK+Y+  FGY++   +  +D+
Sbjct: 34  AEQSTAKATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSELKRYLHRFGYVNDGSEIFSDV 93

Query: 84  FDDALESAIKGYQLNFHMKSTGKMDDDTVKQMMIPRCGIADRALSDQK------------ 143
           FD  LESAI  YQ N  +  TG++D  TV  M +PRCG++D  ++               
Sbjct: 94  FDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSDTHMTINNDFLHTTAHYTYF 153

Query: 144 SRQRPFSKTDLTYKFN-SHALISIGEINIRSIFGQAFQIWADVTDFTFEEVPENSPADIE 203
           + +  +++  LTY  + +H L  +   +++++F +AF  W+ V   +FEEV + + AD++
Sbjct: 154 NGKPKWNRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLK 213

Query: 204 IKFGSGRHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKW--------STNLGMDS 263
           I F +G HGD  PFDG  G   H + PE GR    +HLDA E W        S+ + +D 
Sbjct: 214 IGFYAGDHGDGLPFDGVLGTLAHAFAPENGR----LHLDAAETWIVDDDLKGSSEVAVDL 273

Query: 264 ESIAIHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALYGLN 293
           ES+A HEIGHLLGL H+++   A+MYPS+     K  L  DD++ V   LYG N
Sbjct: 274 ESVATHEIGHLLGLGHSSQE-SAVMYPSLRPRTKKVDLTVDDVAGV-LKLYGPN 321

BLAST of Lag0040443 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 159.8 bits (403), Expect = 3.2e-39
Identity = 101/290 (34.83%), Postives = 149/290 (51.38%), Query Frame = 0

Query: 44  HLAGSRKGRYVRGITKLKKYMKEFGYL--SLKQQENDLFDDALESAIKGYQLNFHMKSTG 103
           +  G   G+ V G+ ++KKY + FGY+  +      D FDD L++A++ YQ NF++  TG
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTG 105

Query: 104 KMDDDTVKQMMIPRCGIADRALSD-------QKSRQRPFSKT------------------ 163
           ++D  T++ ++IPRCG  D            +K+ +  FS+T                  
Sbjct: 106 ELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWP 165

Query: 164 ----DLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDFTFEEVPENSPADIEIKFGSG 223
               DLTY F+    ++     ++S+F +AF  W+DVT   F      S +DI I F +G
Sbjct: 166 RNRRDLTYAFDPKNPLT---EEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTG 225

Query: 224 RHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKW----------STNLGMDSESIA 283
            HGD  PFDG  G   H + P +G+     HLDADE W          S    +D ES+A
Sbjct: 226 DHGDGEPFDGVLGTLAHAFSPPSGK----FHLDADENWVVSGDLDSFLSVTAAVDLESVA 285

Query: 284 IHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALYGLN 293
           +HEIGHLLGL H++   ++IMYP+I +G  K  L  DD+  +   LYG N
Sbjct: 286 VHEIGHLLGLGHSSVE-ESIMYPTITTGKRKVDLTNDDVEGIQ-YLYGAN 326

BLAST of Lag0040443 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 159.1 bits (401), Expect = 5.5e-39
Identity = 103/300 (34.33%), Postives = 147/300 (49.00%), Query Frame = 0

Query: 37  NAFHFIEHLAGSRKGRYVRGITKLKKYMKEFGYL---SLKQQENDLFDDALESAIKGYQL 96
           N ++   +  G   G+   G+  LK+Y + FGY+   +L     D FDD L++A++ YQ 
Sbjct: 41  NPWNSFLNFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQR 100

Query: 97  NFHMKSTGKMDDDTVKQMMIPRCGIAD--RALSDQKSRQRPF------------------ 156
           NF +  TG +D+ T+K ++IPRCG  D     S   S ++ F                  
Sbjct: 101 NFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYS 160

Query: 157 ----------SKTDLTYKFNSHALISIGEINIRSIFGQAFQIWADVTDFTFEEVPENSPA 216
                     ++ DLTY F+    ++     ++S+F +AF  W +VT  TF  V   S +
Sbjct: 161 FFPGEPRWPRNRRDLTYAFDPRNALT---EEVKSVFSRAFTRWEEVTPLTFTRVERFSTS 220

Query: 217 DIEIKFGSGRHGDNSPFDGAGGVFGHVWRPETGRKPLEIHLDADEKW-----------ST 276
           DI I F SG HGD  PFDG      H + P TG      HLD +E W           S 
Sbjct: 221 DISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTG----HFHLDGEENWIVSGEGGDGFISV 280

Query: 277 NLGMDSESIAIHEIGHLLGLDHNTENPDAIMYPSIDSGMVKRHLHQDDISAVHAALYGLN 293
           +  +D ES+A+HEIGHLLGL H++    +IMYP+I +G  K  L  DD+  V   LYG N
Sbjct: 281 SEAVDLESVAVHEIGHLLGLGHSSVE-GSIMYPTIRTGRRKVDLTTDDVEGVQ-YLYGAN 331

BLAST of Lag0040443 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 154.5 bits (389), Expect = 1.4e-37
Identity = 93/262 (35.50%), Postives = 141/262 (53.82%), Query Frame = 0

Query: 57  ITKLKKYMKEFGYLSLKQQENDLFDDALESAIKGYQLNFHMKSTGKMDDDTVKQMMIPRC 116
           I ++K++++++GYL   ++ +D+   + E A+  YQ N  +  TGK D DT+ Q+++PRC
Sbjct: 50  IPEIKRHLQQYGYLPQNKESDDV---SFEQALVRYQKNLGLPITGKPDSDTLSQILLPRC 109

Query: 117 GIADRALSDQKSRQRPFS------------------KTDLTYKFNSHALIS-IGEINIRS 176
           G  D    D + +  PF                      LTY F+   L   +   +IR 
Sbjct: 110 GFPD----DVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRR 169

Query: 177 IFGQAFQIWADVTDFTFEEVPENSPADIEIKFGSGRHGDNSPFDGAGGVFGHVWRPETGR 236
           +F +AF  WA V   +F E  +   ADI+I F +G HGD  PFDG  GV  H + PE GR
Sbjct: 170 VFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGR 229

Query: 237 KPLEIHLDADEKWS-------TNLGMDSESIAIHEIGHLLGLDHNTENPDAIMYPSIDSG 293
               +HLD  E W+       +++ +D ES+A+HEIGH+LGL H++   DA MYP++   
Sbjct: 230 ----LHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVK-DAAMYPTLKPR 289