Lag0040399 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0040399 vs. NCBI nr
Match: XP_022922127.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 526.6 bits (1355), Expect = 1.9e-145
Identity = 265/404 (65.59%), Postives = 317/404 (78.47%), Query Frame = 0

Query: 1   MRVGKDLNCCSVSVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDK-- 60
           +++G   +   V++NTFDL   Q+PLVYAG+IP +GFN S SRFC  NSVD+E VK K  
Sbjct: 335 VQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNIGFNGSISRFCLTNSVDKESVKGKIV 394

Query: 61  --------REVGGVRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLP 120
                     +  + GA GIIMQD +  D+TF +P PASHL +++GALI SY     SLP
Sbjct: 395 LCDFFVLPTNLNFLEGAIGIIMQDNNPKDLTFPFPLPASHLGTQEGALISSYANLT-SLP 454

Query: 121 TATILKSMEGKHEKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATD 180
           TATILKS EGK++ TPFVASFSSRGPNPITP+ILKPDLSGPGVEILAAWSPI  PS A +
Sbjct: 455 TATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEE 514

Query: 181 DNRKVFYNIISGTSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPSGEF 240
           D R++ +NIISGTSMSCPHATA AAYVK+FHPSWSPAA+KSALMTTAFPM  D NP  EF
Sbjct: 515 DTRQLLFNIISGTSMSCPHATAVAAYVKTFHPSWSPAALKSALMTTAFPMRSDLNPDAEF 574

Query: 241 GYGSGHINPLGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTAFD 300
            YGSGHINPLGAVNPGLIYNA+EIDYV+FLCGQGYST+++QQVSGD+S CS  +S+  FD
Sbjct: 575 AYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSNVVFD 634

Query: 301 LNYPSFALSTLILKPINQIYKRSVTNVGSSSTYQATILNLPKDLNITVKPNIISFKALEE 360
           LNYPSFALST I  PI+Q+Y+R VTNVGS+++    I++ P  L ITV P+++SFKAL E
Sbjct: 635 LNYPSFALSTSISTPISQVYRRRVTNVGSANSTYNAIVSGPSRLKITVNPSVLSFKALGE 694

Query: 361 EQSFELIIEGKISSNFVSASLLWDDGEHKVISPIIVFNAVVFTN 395
           E SFE+ IEG ISS  VSASL+WDDG+HKV SP+IVF++  F N
Sbjct: 695 ELSFEVTIEGSISSGIVSASLVWDDGQHKVRSPVIVFDSTSFIN 737

BLAST of Lag0040399 vs. NCBI nr
Match: XP_038874300.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 526.2 bits (1354), Expect = 2.5e-145
Identity = 272/394 (69.04%), Postives = 311/394 (78.93%), Query Frame = 0

Query: 12  VSVNTFDLKRAQFPLVYAGDIPAV--GFNRSESRFCKKNSVDRELVKDK----------R 71
           V++NTFDL   Q+P VYAG++P V  GFN S SRFC  N+VDRELVK K           
Sbjct: 347 VTINTFDLNGTQYPFVYAGNVPNVTGGFNGSISRFCLGNTVDRELVKGKIALCDILVPQT 406

Query: 72  EVGGVRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLPTATILKSME 131
            +G + GA GIIMQDKS  D+TFS+P PASHL +++GALI SY+    SLPTATI KS+E
Sbjct: 407 LLGSIEGAVGIIMQDKSPKDLTFSFPLPASHLGTQEGALISSYLNLT-SLPTATISKSIE 466

Query: 132 GKHEKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATDDNRKVFYNI 191
           GKHE  PFVASFSSRGPNP TPNILKPDLSGPGVEILAAWSPI+SPS A DDNRK+ +NI
Sbjct: 467 GKHEAAPFVASFSSRGPNPTTPNILKPDLSGPGVEILAAWSPISSPSTAKDDNRKLLFNI 526

Query: 192 ISGTSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPSGEFGYGSGHINP 251
           ISGTSM+CPHATA AAYVKSFHPSWSPAA+KSALMTTAFPM  + N   EF YGSGHINP
Sbjct: 527 ISGTSMACPHATAVAAYVKSFHPSWSPAALKSALMTTAFPMRAELNKDAEFAYGSGHINP 586

Query: 252 LGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTAFDLNYPSFALS 311
           LGAVNPGLIYNASEIDY++FLCG+GY+T +LQ++  D+S CSP NSD  FDLNYPSFALS
Sbjct: 587 LGAVNPGLIYNASEIDYIRFLCGEGYNTTLLQRIFEDNSTCSPTNSDQVFDLNYPSFALS 646

Query: 312 TLILKPINQIYKRSVTNVGS-SSTYQATILNLPKDLNITVKPNIISFKALEEEQSFELII 371
           T I    NQ YKR VTNVGS +STY+AT    P  +NI+V P+I+SFKAL EE  FELII
Sbjct: 647 THISTSFNQTYKRRVTNVGSTNSTYKATTF-APSGINISVNPSILSFKALGEELKFELII 706

Query: 372 EGKISSNFVSASLLWDDGEHKVISPIIVFNAVVF 393
           EGKIS +  SASL+WDDG HKV SPIIVF++  F
Sbjct: 707 EGKISRSIESASLVWDDGVHKVRSPIIVFDSDPF 738

BLAST of Lag0040399 vs. NCBI nr
Match: KAG6579374.1 (hypothetical protein SDJN03_23822, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 525.0 bits (1351), Expect = 5.5e-145
Identity = 271/405 (66.91%), Postives = 317/405 (78.27%), Query Frame = 0

Query: 1   MRVGKDLNCCSVSVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDK-- 60
           +++G   +   V+VNTFDL   Q+PLVYAG+IP VGFN S SRFC  NSVD+E VK K  
Sbjct: 335 VQLGDGRSFDGVTVNTFDLNGTQYPLVYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIV 394

Query: 61  --------REVGGVRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLP 120
                     +  + GA GIIMQD +  D+TF +P PASHL +++GALI SY     SLP
Sbjct: 395 LCDFFISPTNLSFLEGAIGIIMQDNNPKDLTFPFPLPASHLGTQEGALISSYANLT-SLP 454

Query: 121 TATILKSMEGKHEKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATD 180
           TATILKS EGK++ TPFVASFSSRGPNPITP+ILKPDLSGPGVEILAAWSPI  PS A +
Sbjct: 455 TATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEE 514

Query: 181 DNRKVFYNIISGTSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPSGEF 240
           D R++ +NIISGTSMSCPHATA AAYVKSFHPSWSPAA+KSALMTTAFPM  D NP  EF
Sbjct: 515 DTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAFPMRSDLNPDAEF 574

Query: 241 GYGSGHINPLGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTAFD 300
            YGSGHINPLGAVNPGLIYNA+EIDYV+FLCGQGYST+++QQVSGD+S CS  +SD  FD
Sbjct: 575 AYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFD 634

Query: 301 LNYPSFALSTLILKPINQIYKRSVTNVGS-SSTYQATILNLPKDLNITVKPNIISFKALE 360
           LNYPSFALST I   I+Q+Y+R VTNVGS +S Y+AT+   P  L ITV P+++SFKAL 
Sbjct: 635 LNYPSFALSTPISTSISQVYRRKVTNVGSANSIYKATVSG-PSGLKITVNPSVLSFKALG 694

Query: 361 EEQSFELIIEGKISSNFVSASLLWDDGEHKVISPIIVFNAVVFTN 395
           EE SFE+ IEG ISS+  SASL+WDDG+HKV SPIIVF++  F N
Sbjct: 695 EELSFEVTIEGSISSSIASASLVWDDGQHKVRSPIIVFDSNKFIN 737

BLAST of Lag0040399 vs. NCBI nr
Match: XP_038891432.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 521.9 bits (1343), Expect = 4.7e-144
Identity = 270/402 (67.16%), Postives = 320/402 (79.60%), Query Frame = 0

Query: 4   GKDLNCCSVSVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDKREV-- 63
           G++ N   VS+NTFDLK  Q PLVYAGDIP   F+ S SR C +N+V+ ELVK K  V  
Sbjct: 342 GREFN--GVSINTFDLKGKQIPLVYAGDIPKAPFDSSVSRMCFENTVELELVKGKIVVCD 401

Query: 64  -----GGV---RGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLPTAT 123
                GGV   +GAAGIIMQD S  D T S+P PASHL  K GALI SYI S +SLPTAT
Sbjct: 402 SLAVPGGVVAIKGAAGIIMQDDSPQDDTNSFPIPASHLAPKPGALILSYINSTNSLPTAT 461

Query: 124 ILKSMEGKHEKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATDDNR 183
           I+KS E K ++ PFVASFSSRGPNPITPNILKPDL+GPGVEILAAW  IASPS A +D++
Sbjct: 462 IMKSTERKRKRAPFVASFSSRGPNPITPNILKPDLTGPGVEILAAWPSIASPSGAEEDSK 521

Query: 184 KVFYNIISGTSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPSGEFGYG 243
           +V YNI+SGTSM+CPH TAAAAYVKSFHP+WSPAA+KSAL+TTAF MSL  NP  EFGYG
Sbjct: 522 RVAYNILSGTSMACPHVTAAAAYVKSFHPTWSPAALKSALITTAFSMSLKRNPDKEFGYG 581

Query: 244 SGHINPLGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTAFDLNY 303
           +GHINPLGAV PGL+YNASEIDYVKFLCGQGY+TE+LQ+VS D+  CS +NSDT FDLNY
Sbjct: 582 AGHINPLGAVQPGLVYNASEIDYVKFLCGQGYNTELLQRVSEDNDTCSSNNSDTVFDLNY 641

Query: 304 PSFALSTLILKPINQIYKRSVTNVGSS-STYQATILNLPKDLNITVKPNIISFKALEEEQ 363
           PSFALST I K INQ+YKR VTNVGS  +TY+ATI+N  K+L ITV P+++SFK L EEQ
Sbjct: 642 PSFALSTNISKSINQVYKRIVTNVGSKYATYKATIINPWKNLEITVNPSVLSFKNLGEEQ 701

Query: 364 SFELIIEGKISSNFVSASLLWDDGEHKVISPIIVFNAVVFTN 395
           +F L I+G+IS N  SASL+W+DG+HKV SPI +F++ + ++
Sbjct: 702 TFVLTIKGRISKNIESASLVWEDGKHKVRSPITIFDSNIHSS 741

BLAST of Lag0040399 vs. NCBI nr
Match: XP_023550545.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521.9 bits (1343), Expect = 4.7e-144
Identity = 264/404 (65.35%), Postives = 314/404 (77.72%), Query Frame = 0

Query: 1   MRVGKDLNCCSVSVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDK-- 60
           +++G   +   V++NTFDL   Q+PLVYAG+IP +GFN S SRFC  NSVD+ELVK K  
Sbjct: 335 VQLGDGRSFDGVTINTFDLNGTQYPLVYAGNIPNIGFNGSISRFCLTNSVDKELVKGKIV 394

Query: 61  --------REVGGVRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLP 120
                     +  + GA GIIMQD +  D+TF +P PASHL +++GALI SY     SLP
Sbjct: 395 LCDFFVSPTNLSFLEGAIGIIMQDNNPKDLTFPFPLPASHLGTQEGALISSYANLT-SLP 454

Query: 121 TATILKSMEGKHEKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATD 180
           TATILKS EGK+++TPFVASFSSRGPNPITP+ILKPDLSGPGVEILAAWSPI  PS A D
Sbjct: 455 TATILKSTEGKYKETPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAED 514

Query: 181 DNRKVFYNIISGTSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPSGEF 240
           D R++ +NIISGTSMSCPHATA AAYVKSFHPSWSPAA+KSALMTTAFPM  D NP  EF
Sbjct: 515 DTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAFPMRADLNPDAEF 574

Query: 241 GYGSGHINPLGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTAFD 300
            YGSGHINPL AVNPGLIYNA+EIDYV+FLCGQGYST+++QQVSGD+S CS  + D  FD
Sbjct: 575 AYGSGHINPLSAVNPGLIYNATEIDYVRFLCGQGYSTKLVQQVSGDNSSCSRGDIDLVFD 634

Query: 301 LNYPSFALSTLILKPINQIYKRSVTNVGSSSTYQATILNLPKDLNITVKPNIISFKALEE 360
           LNYPSFALST I   I+Q+Y+R VTNVGS+++    I++ P  LNITV P+++SFKAL E
Sbjct: 635 LNYPSFALSTSISTSISQVYRRRVTNVGSANSTYKAIVSGPLGLNITVNPSVLSFKALGE 694

Query: 361 EQSFELIIEGKISSNFVSASLLWDDGEHKVISPIIVFNAVVFTN 395
           E  FE+ IEG ISS+  S SL+WDDG+HKV SPI+VF+   F N
Sbjct: 695 ELRFEVTIEGSISSSIASGSLVWDDGKHKVKSPIVVFDENTFIN 737

BLAST of Lag0040399 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 3.8e-96
Identity = 194/402 (48.26%), Postives = 255/402 (63.43%), Query Frame = 0

Query: 1   MRVGKDLNCCSVSVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDKRE 60
           +++G   +   VS+NTFD     +PLV   DIP  GF++S SRFC   SV+  L+K K  
Sbjct: 338 VQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIV 397

Query: 61  V-----------GGVRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSL 120
           V             + GAAG++M   +  D   SYP P+S L   D      YI S  S 
Sbjct: 398 VCEASFGPHEFFKSLDGAAGVLMTSNTR-DYADSYPLPSSVLDPNDLLATLRYIYSIRS- 457

Query: 121 PTATILKSMEGKHEKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRAT 180
           P ATI KS    +   P V SFSSRGPN  T +++KPD+SGPGVEILAAW  +A      
Sbjct: 458 PGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGI- 517

Query: 181 DDNRKVFYNIISGTSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPSGE 240
              R   +NIISGTSMSCPH T  A YVK+++P+WSPAAIKSALMTTA PM+  FNP  E
Sbjct: 518 --RRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAE 577

Query: 241 FGYGSGHINPLGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTAF 300
           F YGSGH+NPL AV PGL+Y+A+E DYVKFLCGQGY+T+ +++++GD S C+  N+   +
Sbjct: 578 FAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVW 637

Query: 301 DLNYPSFALSTLILKPINQIYKRSVTNVG-SSSTYQATILNLPKDLNITVKPNIISFKAL 360
           DLNYPSF LS    +  NQ + R++T+V   +STY+A +++ P+ L I+V PN++SF  L
Sbjct: 638 DLNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRA-MISAPQGLTISVNPNVLSFNGL 697

Query: 361 EEEQSFELIIEGKISSNFVSASLLWDDGEHKVISPIIVFNAV 391
            + +SF L + G I    VSASL+W DG H V SPI + + V
Sbjct: 698 GDRKSFTLTVRGSIKGFVVSASLVWSDGVHYVRSPITITSLV 731

BLAST of Lag0040399 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 3.5e-86
Identity = 179/392 (45.66%), Postives = 247/392 (63.01%), Query Frame = 0

Query: 12  VSVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDK----------REV 71
           +SVNTF+L   +FP+VY  ++ +   +++++ +C    VD ELVK K          RE 
Sbjct: 340 ISVNTFNLNGTKFPIVYGQNV-SRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYRE- 399

Query: 72  GGVRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLPTATILKSMEGK 131
             + GA G+I+Q+    D  F  P+PAS L  +D   I SYI S    P A IL++ E  
Sbjct: 400 AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP-PQAEILRTEEIV 459

Query: 132 HEKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSR--ATDDNRKVFYNI 191
             + P+V SFSSRGP+ +  N+LKPD+S PG+EILAA+SP+ASPS     +D R V Y++
Sbjct: 460 DREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSV 519

Query: 192 ISGTSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPSGEFGYGSGHINP 251
           +SGTSM+CPH    AAYVKSFHP WSP+AIKSA+MTTA PM+L  NP  EF YGSG INP
Sbjct: 520 MSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINP 579

Query: 252 LGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTAFDLNYPSFALS 311
             A +PGL+Y     DY+K LC +G+ +  L   SG +  CS        DLNYP+    
Sbjct: 580 TKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCS--ERTEVKDLNYPTMTTF 639

Query: 312 TLILKPINQIYKRSVTNVG-SSSTYQATILNLPKDLNITVKPNIISFKALEEEQSFELII 371
              L P N  +KR+VTNVG  +STY+A+++ L  +L I+++P I+ F  LEE++SF + I
Sbjct: 640 VSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTI 699

Query: 372 EGK--ISSNFVSASLLWDDGEHKVISPIIVFN 389
            GK     +FVS+S++W DG H V SPI+ ++
Sbjct: 700 SGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 726

BLAST of Lag0040399 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 1.2e-81
Identity = 167/391 (42.71%), Postives = 246/391 (62.92%), Query Frame = 0

Query: 13  SVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDKREV---------GG 72
           SVNT+D+    +PLVY         +  ++R C+   +D +LVK K  +           
Sbjct: 350 SVNTYDMNGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQ 409

Query: 73  VRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLPTATILKSMEGKHE 132
             GA G I+++    D  F   +P S L + D   + SY+ S  + P AT+LKS E  ++
Sbjct: 410 KLGAVGSIVKNPEP-DRAFIRSFPVSFLSNDDYKSLVSYMNSTKN-PKATVLKSEEISNQ 469

Query: 133 KTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATDDNRKVFYNIISGT 192
           + P VASFSSRGP+ I  +ILKPD++ PGVEILAA+SP +SP+ +  D R+V Y+++SGT
Sbjct: 470 RAPLVASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGT 529

Query: 193 SMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFN--PSGEFGYGSGHINPLG 252
           SM+CPH    AAYVK+FHP WSP+ I+SA+MTTA+PM+   +   S EF YGSGH++P+ 
Sbjct: 530 SMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPID 589

Query: 253 AVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDT-AFDLNYPSFALST 312
           A+NPGL+Y  ++ D++ FLCG  Y+++ L+ +SGD+S C+ + S T   +LNYP+ +   
Sbjct: 590 AINPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKV 649

Query: 313 LILKPINQIYKRSVTNVG-SSSTYQATILNLP-KDLNITVKPNIISFKALEEEQSFELII 372
              KP N  ++R+VTNVG   STY A ++  P   L+I V P ++S K++ E+QSF + +
Sbjct: 650 SGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTV 709

Query: 373 EGKI--SSNFVSASLLWDDGEHKVISPIIVF 388
                 +   VSA+L+W DG H V SPIIV+
Sbjct: 710 SSDSIGTKQPVSANLIWSDGTHNVRSPIIVY 738

BLAST of Lag0040399 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 5.1e-77
Identity = 164/391 (41.94%), Postives = 242/391 (61.89%), Query Frame = 0

Query: 13  SVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDK-------REVGGVR 72
           SVN+FDL   ++PLVY G   +   + S + FC    +D + VK K       R  G  +
Sbjct: 352 SVNSFDLNGKKYPLVY-GKSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQ 411

Query: 73  --GAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLPTATILKSMEGKHE 132
             GA   I+++    D    + +P S L   D  ++ SY+ S  + P A +LKS    ++
Sbjct: 412 AMGAVASIVRNPYE-DAASVFSFPVSVLSEDDYNIVLSYVNSTKN-PKAAVLKSETIFNQ 471

Query: 133 KTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATDDNRKVFYNIISGT 192
           K P VAS+SSRGPNP+  +ILKPD++ PG EILAA+SP   PS +  D R V Y +ISGT
Sbjct: 472 KAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSES--DTRHVKYTVISGT 531

Query: 193 SMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPS---GEFGYGSGHINPL 252
           SMSCPH    AAY+K+FHP WSP+ I+SA+MTTA+PM+   +PS    EF YG+GH++P+
Sbjct: 532 SMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPI 591

Query: 253 GAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDT-AFDLNYPSFALS 312
            A++PGL+Y A++ D++ FLCG  Y+ + L+ +SGD S C+ + + +   +LNYPS +  
Sbjct: 592 AAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQ 651

Query: 313 TLILKPINQIYKRSVTNVG-SSSTYQATILNLPKDLNITVKPNIISFKALEEEQSFELII 372
               KP    ++R+VTNVG  ++TY+A ++     L + V P ++S K+L E++SF + +
Sbjct: 652 VSGTKPFKVTFRRTVTNVGRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKKSFTVTV 711

Query: 373 E--GKISSNFVSASLLWDDGEHKVISPIIVF 388
              G  + N VSA L+W DG H V SPI+V+
Sbjct: 712 SGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of Lag0040399 vs. ExPASy Swiss-Prot
Match: F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.7e-75
Identity = 160/392 (40.82%), Postives = 237/392 (60.46%), Query Frame = 0

Query: 13  SVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDK----------REVG 72
           SVN+FDL   ++PLVY G   +     + + FC    +D + VK K           E  
Sbjct: 352 SVNSFDLNGKKYPLVY-GKSASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQ 411

Query: 73  GVRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLPTATILKSMEGKH 132
            +   A I+   ++  D+   + +P S L   D   + SY+ S  + P A +LKS    +
Sbjct: 412 AMGAIASIVRSHRT--DVASIFSFPVSVLLEDDYNTVLSYMNSTKN-PKAAVLKSETIFN 471

Query: 133 EKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATDDNRKVFYNIISG 192
           ++ P VAS+ SRGPN I P+ILKPD++ PG EI+AA+SP A PS    D R+V Y++ +G
Sbjct: 472 QRAPVVASYFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTG 531

Query: 193 TSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLD---FNPSGEFGYGSGHINP 252
           TSMSCPH    AAY+KSFHP WSP+ I+SA+MTTA+PM+     FN   EF YG+GH++P
Sbjct: 532 TSMSCPHVAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDP 591

Query: 253 LGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDT-AFDLNYPSFAL 312
           + A++PGL+Y A++ D++ FLCG  Y+ + L+ +SGD S C+ + + +   +LNYPS   
Sbjct: 592 ITAIHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTA 651

Query: 313 STLILKPINQIYKRSVTNVG-SSSTYQATILNLPKDLNITVKPNIISFKALEEEQSFELI 372
                KP   I++R+VTNVG  ++TY+A ++     L + V P ++S K+L E++SF + 
Sbjct: 652 QVSAAKPFKVIFRRTVTNVGRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKKSFTVT 711

Query: 373 IE--GKISSNFVSASLLWDDGEHKVISPIIVF 388
               G  + N VSA L+W DG H V SPI+V+
Sbjct: 712 ASGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of Lag0040399 vs. ExPASy TrEMBL
Match: A0A6J1E2C5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430194 PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 9.2e-146
Identity = 265/404 (65.59%), Postives = 317/404 (78.47%), Query Frame = 0

Query: 1   MRVGKDLNCCSVSVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDK-- 60
           +++G   +   V++NTFDL   Q+PLVYAG+IP +GFN S SRFC  NSVD+E VK K  
Sbjct: 335 VQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNIGFNGSISRFCLTNSVDKESVKGKIV 394

Query: 61  --------REVGGVRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLP 120
                     +  + GA GIIMQD +  D+TF +P PASHL +++GALI SY     SLP
Sbjct: 395 LCDFFVLPTNLNFLEGAIGIIMQDNNPKDLTFPFPLPASHLGTQEGALISSYANLT-SLP 454

Query: 121 TATILKSMEGKHEKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATD 180
           TATILKS EGK++ TPFVASFSSRGPNPITP+ILKPDLSGPGVEILAAWSPI  PS A +
Sbjct: 455 TATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEE 514

Query: 181 DNRKVFYNIISGTSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPSGEF 240
           D R++ +NIISGTSMSCPHATA AAYVK+FHPSWSPAA+KSALMTTAFPM  D NP  EF
Sbjct: 515 DTRQLLFNIISGTSMSCPHATAVAAYVKTFHPSWSPAALKSALMTTAFPMRSDLNPDAEF 574

Query: 241 GYGSGHINPLGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTAFD 300
            YGSGHINPLGAVNPGLIYNA+EIDYV+FLCGQGYST+++QQVSGD+S CS  +S+  FD
Sbjct: 575 AYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSNVVFD 634

Query: 301 LNYPSFALSTLILKPINQIYKRSVTNVGSSSTYQATILNLPKDLNITVKPNIISFKALEE 360
           LNYPSFALST I  PI+Q+Y+R VTNVGS+++    I++ P  L ITV P+++SFKAL E
Sbjct: 635 LNYPSFALSTSISTPISQVYRRRVTNVGSANSTYNAIVSGPSRLKITVNPSVLSFKALGE 694

Query: 361 EQSFELIIEGKISSNFVSASLLWDDGEHKVISPIIVFNAVVFTN 395
           E SFE+ IEG ISS  VSASL+WDDG+HKV SP+IVF++  F N
Sbjct: 695 ELSFEVTIEGSISSGIVSASLVWDDGQHKVRSPVIVFDSTSFIN 737

BLAST of Lag0040399 vs. ExPASy TrEMBL
Match: A0A6J1E2G4 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 6.6e-144
Identity = 265/406 (65.27%), Postives = 315/406 (77.59%), Query Frame = 0

Query: 1   MRVGKDLNCCSVSVNTFDLKRAQFPLVYAGDIPAV--GFNRSESRFCKKNSVDRELVKDK 60
           +++G   +   V++NTFDL   Q+PLVYAG+IP +  GFN S SRFC  NSVDRE VK K
Sbjct: 335 IQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNITGGFNGSISRFCLPNSVDRESVKGK 394

Query: 61  ----------REVGGVRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHS 120
                     + +G + GA GIIMQD +  D+TF +P PASHL +++GALI SY      
Sbjct: 395 IVLCDFFVSPKNLGSLEGAIGIIMQDTNPKDLTFPFPLPASHLGTQEGALISSYANLT-G 454

Query: 121 LPTATILKSMEGKHEKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRA 180
           LPTATILKS EGK++ TPFVASFSSRGPNPITP+ILKPDLSGPGVEILAAWSPI  PS A
Sbjct: 455 LPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGA 514

Query: 181 TDDNRKVFYNIISGTSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPSG 240
            +D R++ +NIISGTSMSCPHATA AAYVK+FHPSWSPAA+KSALMTTAFPM  D NP  
Sbjct: 515 EEDTRQLLFNIISGTSMSCPHATAVAAYVKTFHPSWSPAALKSALMTTAFPMRADLNPDA 574

Query: 241 EFGYGSGHINPLGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTA 300
           EF YGSGHINPL AVNPGLIYNA+EIDYV+FLCGQGYST+++QQVSGD+S CS  + D  
Sbjct: 575 EFAYGSGHINPLSAVNPGLIYNATEIDYVRFLCGQGYSTKLVQQVSGDNSSCSRGDIDLV 634

Query: 301 FDLNYPSFALSTLILKPINQIYKRSVTNVGSSSTYQATILNLPKDLNITVKPNIISFKAL 360
           FDLNYPSFALST I   I+Q+Y+R VTNVGS+++    I++ P  LNITV P+++SFKAL
Sbjct: 635 FDLNYPSFALSTSISTSISQVYRRRVTNVGSANSTYKAIVSGPSGLNITVNPSVLSFKAL 694

Query: 361 EEEQSFELIIEGKISSNFVSASLLWDDGEHKVISPIIVFNAVVFTN 395
            EE SFEL IEG ISS+  SASL+WDDG+HKV SPI+VF+   F N
Sbjct: 695 GEELSFELTIEGSISSSIASASLVWDDGKHKVKSPIVVFDENTFIN 739

BLAST of Lag0040399 vs. ExPASy TrEMBL
Match: A0A0A0KWL9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G613590 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 9.5e-143
Identity = 266/400 (66.50%), Postives = 315/400 (78.75%), Query Frame = 0

Query: 1   MRVGKDLNCCSVSVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDKRE 60
           + +G       VSVNTFD+K  Q PLVYAGDIP   F+ S SR C +N+VD +LVK K  
Sbjct: 338 VELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIV 397

Query: 61  V-------GG---VRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLP 120
           V       GG   V+GA GIIMQD S+ D T S+P PASHL  K GAL+ SYI S +S+P
Sbjct: 398 VCDSLTVPGGVVAVKGAVGIIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIP 457

Query: 121 TATILKSMEGKHEKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATD 180
           TATI KS E K ++ P VASFSSRGPNPITPNILKPDLSGPGVEILAAWSP++ PS A +
Sbjct: 458 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEE 517

Query: 181 DNRKVFYNIISGTSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPSGEF 240
           DN++V YNIISGTSM+CPH TAAAAYVKSFHP+WSP+A+KSAL+TTAFPMS   NP  EF
Sbjct: 518 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEF 577

Query: 241 GYGSGHINPLGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTAFD 300
           GYG+GHINPLGAV+PGLIY+ASEIDYV+FLCGQGY+TE+LQ VS D++ CS +NSDT FD
Sbjct: 578 GYGAGHINPLGAVHPGLIYDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFD 637

Query: 301 LNYPSFALSTLILKPINQIYKRSVTNVGSS-STYQATILNLPKDLNITVKPNIISFKALE 360
           LNYPSFALST I KPINQ+YKR+VTNVGS  +TY+AT++N  K+L I V P+++SFK L 
Sbjct: 638 LNYPSFALSTNISKPINQVYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLG 697

Query: 361 EEQSFELIIEGKISSNFVSASLLWDDGEHKVISPIIVFNA 390
           E+QSFE+ I GKI  +  SASL+WDDG+HKV SPI VF A
Sbjct: 698 EKQSFEVTIRGKIRKDIESASLVWDDGKHKVRSPITVFIA 737

BLAST of Lag0040399 vs. ExPASy TrEMBL
Match: A0A5A7SZX9 (Cucumisin-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G006680 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.2e-142
Identity = 266/402 (66.17%), Postives = 318/402 (79.10%), Query Frame = 0

Query: 4   GKDLNCCSVSVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDK----- 63
           G++ N   VSVNTFD+K  Q PLVYAGDIP   F+ S SR C +N++D +LVK K     
Sbjct: 342 GREFN--GVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTIDLKLVKGKIVVCD 401

Query: 64  -----REVGGVRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLPTAT 123
                  V  V+GA GIIMQD S+ D T S+P PASHL  + GALI SYI S +S+PTAT
Sbjct: 402 SLTVPGGVAAVKGAVGIIMQDDSSPDDTNSFPIPASHLGPEAGALILSYINSTNSIPTAT 461

Query: 124 ILKSMEGKHEKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATDDNR 183
           I KS E K ++ P VASFSSRGPNPITPNILKPDLSGPGVEILAAWSPI+ PS A +D++
Sbjct: 462 IKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPISPPSGAEEDSK 521

Query: 184 KVFYNIISGTSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPSGEFGYG 243
           +V YNIISGTSM+CPH TAAAAYVKSFHP+WSP+A+KSAL+TTAF MS   N   EFGYG
Sbjct: 522 RVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEFGYG 581

Query: 244 SGHINPLGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTAFDLNY 303
           +GHINPLGAV+PGLIY+ASEIDYV FLCGQGY+TE+LQQVS D++ CS +NSDT FDLNY
Sbjct: 582 AGHINPLGAVHPGLIYDASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFDLNY 641

Query: 304 PSFALSTLILKPINQIYKRSVTNVGS-SSTYQATILNLPKDLNITVKPNIISFKALEEEQ 363
           PSFALST I KPINQ+Y+R+VTNVGS S+TY+ATI+N  K+L I V P+++SFK L EEQ
Sbjct: 642 PSFALSTNISKPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLGEEQ 701

Query: 364 SFELIIEGKISSNFVSASLLWDDGEHKVISPIIVFNAVVFTN 395
           SFE+ I+GKI  N  SASL+WDDG+HKV SPI VF+A + ++
Sbjct: 702 SFEVTIKGKIRKNIESASLVWDDGKHKVRSPITVFDANIHSS 741

BLAST of Lag0040399 vs. ExPASy TrEMBL
Match: A0A1S4DW16 (cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489381 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.2e-142
Identity = 266/402 (66.17%), Postives = 318/402 (79.10%), Query Frame = 0

Query: 4   GKDLNCCSVSVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDK----- 63
           G++ N   VSVNTFD+K  Q PLVYAGDIP   F+ S SR C +N++D +LVK K     
Sbjct: 342 GREFN--GVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTIDLKLVKGKIVVCD 401

Query: 64  -----REVGGVRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLPTAT 123
                  V  V+GA GIIMQD S+ D T S+P PASHL  + GALI SYI S +S+PTAT
Sbjct: 402 SLTVPGGVAAVKGAVGIIMQDDSSPDDTNSFPIPASHLGPEAGALILSYINSTNSIPTAT 461

Query: 124 ILKSMEGKHEKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATDDNR 183
           I KS E K ++ P VASFSSRGPNPITPNILKPDLSGPGVEILAAWSPI+ PS A +D++
Sbjct: 462 IKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPISPPSGAEEDSK 521

Query: 184 KVFYNIISGTSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPSGEFGYG 243
           +V YNIISGTSM+CPH TAAAAYVKSFHP+WSP+A+KSAL+TTAF MS   N   EFGYG
Sbjct: 522 RVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEFGYG 581

Query: 244 SGHINPLGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTAFDLNY 303
           +GHINPLGAV+PGLIY+ASEIDYV FLCGQGY+TE+LQQVS D++ CS +NSDT FDLNY
Sbjct: 582 AGHINPLGAVHPGLIYDASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFDLNY 641

Query: 304 PSFALSTLILKPINQIYKRSVTNVGS-SSTYQATILNLPKDLNITVKPNIISFKALEEEQ 363
           PSFALST I KPINQ+Y+R+VTNVGS S+TY+ATI+N  K+L I V P+++SFK L EEQ
Sbjct: 642 PSFALSTNISKPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLGEEQ 701

Query: 364 SFELIIEGKISSNFVSASLLWDDGEHKVISPIIVFNAVVFTN 395
           SFE+ I+GKI  N  SASL+WDDG+HKV SPI VF+A + ++
Sbjct: 702 SFEVTIKGKIRKNIESASLVWDDGKHKVRSPITVFDANIHSS 741

BLAST of Lag0040399 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 320.1 bits (819), Expect = 2.5e-87
Identity = 179/392 (45.66%), Postives = 247/392 (63.01%), Query Frame = 0

Query: 12  VSVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDK----------REV 71
           +SVNTF+L   +FP+VY  ++ +   +++++ +C    VD ELVK K          RE 
Sbjct: 304 ISVNTFNLNGTKFPIVYGQNV-SRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYRE- 363

Query: 72  GGVRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLPTATILKSMEGK 131
             + GA G+I+Q+    D  F  P+PAS L  +D   I SYI S    P A IL++ E  
Sbjct: 364 AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP-PQAEILRTEEIV 423

Query: 132 HEKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSR--ATDDNRKVFYNI 191
             + P+V SFSSRGP+ +  N+LKPD+S PG+EILAA+SP+ASPS     +D R V Y++
Sbjct: 424 DREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSV 483

Query: 192 ISGTSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPSGEFGYGSGHINP 251
           +SGTSM+CPH    AAYVKSFHP WSP+AIKSA+MTTA PM+L  NP  EF YGSG INP
Sbjct: 484 MSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINP 543

Query: 252 LGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTAFDLNYPSFALS 311
             A +PGL+Y     DY+K LC +G+ +  L   SG +  CS        DLNYP+    
Sbjct: 544 TKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCS--ERTEVKDLNYPTMTTF 603

Query: 312 TLILKPINQIYKRSVTNVG-SSSTYQATILNLPKDLNITVKPNIISFKALEEEQSFELII 371
              L P N  +KR+VTNVG  +STY+A+++ L  +L I+++P I+ F  LEE++SF + I
Sbjct: 604 VSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTI 663

Query: 372 EGK--ISSNFVSASLLWDDGEHKVISPIIVFN 389
            GK     +FVS+S++W DG H V SPI+ ++
Sbjct: 664 SGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 690

BLAST of Lag0040399 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 305.1 bits (780), Expect = 8.4e-83
Identity = 167/391 (42.71%), Postives = 246/391 (62.92%), Query Frame = 0

Query: 13  SVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDKREV---------GG 72
           SVNT+D+    +PLVY         +  ++R C+   +D +LVK K  +           
Sbjct: 350 SVNTYDMNGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQ 409

Query: 73  VRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLPTATILKSMEGKHE 132
             GA G I+++    D  F   +P S L + D   + SY+ S  + P AT+LKS E  ++
Sbjct: 410 KLGAVGSIVKNPEP-DRAFIRSFPVSFLSNDDYKSLVSYMNSTKN-PKATVLKSEEISNQ 469

Query: 133 KTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATDDNRKVFYNIISGT 192
           + P VASFSSRGP+ I  +ILKPD++ PGVEILAA+SP +SP+ +  D R+V Y+++SGT
Sbjct: 470 RAPLVASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGT 529

Query: 193 SMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFN--PSGEFGYGSGHINPLG 252
           SM+CPH    AAYVK+FHP WSP+ I+SA+MTTA+PM+   +   S EF YGSGH++P+ 
Sbjct: 530 SMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPID 589

Query: 253 AVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDT-AFDLNYPSFALST 312
           A+NPGL+Y  ++ D++ FLCG  Y+++ L+ +SGD+S C+ + S T   +LNYP+ +   
Sbjct: 590 AINPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKV 649

Query: 313 LILKPINQIYKRSVTNVG-SSSTYQATILNLP-KDLNITVKPNIISFKALEEEQSFELII 372
              KP N  ++R+VTNVG   STY A ++  P   L+I V P ++S K++ E+QSF + +
Sbjct: 650 SGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTV 709

Query: 373 EGKI--SSNFVSASLLWDDGEHKVISPIIVF 388
                 +   VSA+L+W DG H V SPIIV+
Sbjct: 710 SSDSIGTKQPVSANLIWSDGTHNVRSPIIVY 738

BLAST of Lag0040399 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 289.7 bits (740), Expect = 3.6e-78
Identity = 164/391 (41.94%), Postives = 242/391 (61.89%), Query Frame = 0

Query: 13  SVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDK-------REVGGVR 72
           SVN+FDL   ++PLVY G   +   + S + FC    +D + VK K       R  G  +
Sbjct: 352 SVNSFDLNGKKYPLVY-GKSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQ 411

Query: 73  --GAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLPTATILKSMEGKHE 132
             GA   I+++    D    + +P S L   D  ++ SY+ S  + P A +LKS    ++
Sbjct: 412 AMGAVASIVRNPYE-DAASVFSFPVSVLSEDDYNIVLSYVNSTKN-PKAAVLKSETIFNQ 471

Query: 133 KTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATDDNRKVFYNIISGT 192
           K P VAS+SSRGPNP+  +ILKPD++ PG EILAA+SP   PS +  D R V Y +ISGT
Sbjct: 472 KAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSES--DTRHVKYTVISGT 531

Query: 193 SMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFNPS---GEFGYGSGHINPL 252
           SMSCPH    AAY+K+FHP WSP+ I+SA+MTTA+PM+   +PS    EF YG+GH++P+
Sbjct: 532 SMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPI 591

Query: 253 GAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDT-AFDLNYPSFALS 312
            A++PGL+Y A++ D++ FLCG  Y+ + L+ +SGD S C+ + + +   +LNYPS +  
Sbjct: 592 AAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQ 651

Query: 313 TLILKPINQIYKRSVTNVG-SSSTYQATILNLPKDLNITVKPNIISFKALEEEQSFELII 372
               KP    ++R+VTNVG  ++TY+A ++     L + V P ++S K+L E++SF + +
Sbjct: 652 VSGTKPFKVTFRRTVTNVGRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKKSFTVTV 711

Query: 373 E--GKISSNFVSASLLWDDGEHKVISPIIVF 388
              G  + N VSA L+W DG H V SPI+V+
Sbjct: 712 SGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of Lag0040399 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 283.5 bits (724), Expect = 2.6e-76
Identity = 160/392 (40.82%), Postives = 237/392 (60.46%), Query Frame = 0

Query: 13  SVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDK----------REVG 72
           SVN+FDL   ++PLVY G   +     + + FC    +D + VK K           E  
Sbjct: 352 SVNSFDLNGKKYPLVY-GKSASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQ 411

Query: 73  GVRGAAGIIMQDKSALDITFSYPWPASHLPSKDGALIFSYITSNHSLPTATILKSMEGKH 132
            +   A I+   ++  D+   + +P S L   D   + SY+ S  + P A +LKS    +
Sbjct: 412 AMGAIASIVRSHRT--DVASIFSFPVSVLLEDDYNTVLSYMNSTKN-PKAAVLKSETIFN 471

Query: 133 EKTPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATDDNRKVFYNIISG 192
           ++ P VAS+ SRGPN I P+ILKPD++ PG EI+AA+SP A PS    D R+V Y++ +G
Sbjct: 472 QRAPVVASYFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTG 531

Query: 193 TSMSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLD---FNPSGEFGYGSGHINP 252
           TSMSCPH    AAY+KSFHP WSP+ I+SA+MTTA+PM+     FN   EF YG+GH++P
Sbjct: 532 TSMSCPHVAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDP 591

Query: 253 LGAVNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDT-AFDLNYPSFAL 312
           + A++PGL+Y A++ D++ FLCG  Y+ + L+ +SGD S C+ + + +   +LNYPS   
Sbjct: 592 ITAIHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTA 651

Query: 313 STLILKPINQIYKRSVTNVG-SSSTYQATILNLPKDLNITVKPNIISFKALEEEQSFELI 372
                KP   I++R+VTNVG  ++TY+A ++     L + V P ++S K+L E++SF + 
Sbjct: 652 QVSAAKPFKVIFRRTVTNVGRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKKSFTVT 711

Query: 373 IE--GKISSNFVSASLLWDDGEHKVISPIIVF 388
               G  + N VSA L+W DG H V SPI+V+
Sbjct: 712 ASGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of Lag0040399 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 281.6 bits (719), Expect = 9.9e-76
Identity = 163/394 (41.37%), Postives = 238/394 (60.41%), Query Frame = 0

Query: 13  SVNTFDLKRAQFPLVYAGDIPAVGFNRSESRFCKKNSVDRELVKDKREVGG-------VR 72
           SVN FD+K  ++PLVY     +   +   +  C    +++  VK K  V G        +
Sbjct: 343 SVNAFDMKGKKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAK 402

Query: 73  GAAGIIMQDKSAL-DITFSYPWPASHLPSKDGALIFSYITSNHSLPTATILKSMEGKHEK 132
               I + DKS   D+ F++  PAS L +KD   + SYI S  S P A +LK+    +  
Sbjct: 403 SVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDS-PQAAVLKTETIFNRT 462

Query: 133 TPFVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPIASPSRATDDNRKVFYNIISGTS 192
           +P +ASFSSRGPN I  +ILKPD++ PGVEILAA+SP   PS   DD R+V Y++ SGTS
Sbjct: 463 SPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSE--DDTRRVKYSVFSGTS 522

Query: 193 MSCPHATAAAAYVKSFHPSWSPAAIKSALMTTAFPMSLDFN--PSGEFGYGSGHINPLGA 252
           M+CPH    AAYVK+F+P WSP+ I+SA+MTTA+P+        S EF YG+GH++P+ A
Sbjct: 523 MACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAA 582

Query: 253 VNPGLIYNASEIDYVKFLCGQGYSTEMLQQVSGDDSVCSPDNSDTAFDLNYPSF-ALSTL 312
           +NPGL+Y   + D++ FLCG  Y+++ L+ +SGD   CS  N     +LNYPS  A  + 
Sbjct: 583 LNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSG 642

Query: 313 ILKPINQIYKRSVTNVGS-SSTYQATIL-NLPKDLNITVKPNIISFKALEEEQSFELIIE 372
                +  + R++TNVG+ +STY++ ++      L+I V P+++ FK + E+QSF + + 
Sbjct: 643 TDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVT 702

Query: 373 GKISSNFV--SASLLWDDGEHKVISPIIVFNAVV 392
           G    + V  SA+L+W DG H V SPI+V+  VV
Sbjct: 703 GSDVDSEVPSSANLIWSDGTHNVRSPIVVYIMVV 733