Lag0040398 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040398
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptioncucumisin-like
Locationchr13: 4455069 .. 4464816 (-)
RNA-Seq ExpressionLag0040398
SyntenyLag0040398
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACCAAGGAGGCAAAACCGGCAAATGGGACGGGCCAAGACCGAAGGGGTCGGGTTTTCGGCCCGACCCCCTGCTCGGCCTCGGCCATGGGCCGAGGCCGACCCTCGGCCATGGGCCGAGGCCGACCCTCGGCCCGCTCGTGCGGGCCGAGTCCGTTTGGTCTCGTCTGGTCCCCACCGCCTCTGGATGCCCCGGTTTCGCCTGGTTTGACCTAAAACGCCTCCGAAACCCTAAAAAGGCTAGGAGGATGAACAGGTATTTATATCCCTCTTCGCCACTGAAGAGGGGATCCCGAATTCTATCCCTAAACACTACTCTCGAACTCTGTACTTTCTCCTCTTGCTCTTACTTTTCCACGCCCTACCGTTCTGTTTGCTGACTTAAGCATCGGAGCCGGTGTGGCGAGCACCACACCGGTGTGCAGGTTTACTGTCTTGCAGGCCACGTCTTCCCCCCTCAACTACAAATTTATCGTTGGTGGCACGTGAAGGTCAGGTGAGATCATTTGGCCAAAATCGACCATCAACACACATATATATATAATGTGGAATAAGTTATAATTAATGTATGTCCTTGTCAGATAAATTAATTTCCAACACACACACATATTTGATATTTTCCTTCTCTCCCACAATATTTTATACTTTAAATTGATTTAATCAACTAACATGTTTTTTTTTTTTTTGAAGTAAATCAACTAACACGTTGTAAGGACAAGTTGGGATGCTAATTTAATTTGAATAAAAATCAACACAATTGCAAACTTTCTTGATATTTCAACATTTCAAAATGTAATTGGACAATATGGATTTTTTTTTTAATACATTTTGACAAAGATAATGTTTCAATAGCCACCTCAATCCATAAATAGCTTCTAAACTCATTCATAAATTTCATAACATTTAGAGAGGGAAAAAAAAACCCTCTCTTCCTTTTCCAATCTGAGTGAAAAATCTCAATGTCTTCTCTCTTGAGATTTCTCTTTCTGGGTTTATGTTGTTCACTCTTCTTTTTTAGTTCAAAGTCGCAAGATGATCCTCGAAAGGCAACTCTCTCCCTCTTGTTCTTATACTTGTTCATGTCGTTTAATTTGTTATACTTTCTTCATCTTTTCTTTGTACTACTTAAATCTTGATAATTGATATTTTTTGTTCTATGTGTAGACATATATTGTATATATGGGAAGTCATTCAAAAAACAAAGTTTCAACTTCATTTCATCACATGAGAATACTAGAAGAAGCTATTGGCAGGTGATCCATCATTTTTATTTTTATTATTATATCAATTTTTTACATATTATTGTCAGCTAAATATTTGTGTCTAGAAAATTCGTCACACAAGACGAGTTAATATGAAACCCCCTACGAGATTACAGATCAGAACTTTTGCCATCAAAGGAAGACAGCTCCCAATTATCAAATCCTAAAACCTTTTTTTTTTCTATAATACCTAAAACCCCAAATTGATTTAGCACACAACTCCAAGAAAAGAGTTAGAAAAAGGTTATCTAAAGAACTAATTTAGTTCTTCAAAATGTAAAATGTTTTATTCAAAGTGAACATGTCTCAAGTAAGCCTAATTATAGTATCATCCCACGGCTACTTGCATTCATTTATTCAAACTTAAGTTTTAAATGAGACTTAAAGTCAACAATTTTATAATGTCTCAACCCTAATTATAGTGGTGATGATTCTAAGAAAAAGAGCGACACTAACAACCCTTTGAATAAATATTTGTACTTTAGGGGTAAAAATGAAAAACAAATAATATTACATCAAAACACTCTTAAACAAAAAGTGGATTTTAATATATGTAAAGTCCTTTGGCAATTATTCATATCATAAGACATTTCTAATTAATTTACAAGAAAAAAAAAATCTGTCATTTTTTCAGCAACTTTGCTCCACACTCTTTACTCCATAGCTACAAGAGAAGCTTCAATGGCTTTGTCGCAAAGTTGACTGAAGTTGAAGCACATAAAGTTTCTAGTAAGTTCAAAAAAACCCTTTAATTTTGATTTTTTTCTCTCCAAAATTATGAGCATGAATTTTTATCTTTTTATTCCTATTAGAAATTAATTTATACATATATCTTTAAACTTGATTTTAGAAATGAAAGGTGTAATCTCAATTTTTCCAAATGGAAAAAAGCAACTCCACACGACAAGGTCGTGGGACTTCATGGGTTTCTCCGAACAAGTCAACAGAGTCCCTACGGTGGAAAGCGACATCATTGTCGGAGTACTGGATACCGGCATTTGGCCGGAATCTCCAAGTTTCGATGACAAAGGTTACAGTCCGCCGCCAGCCAAGTGGAACGGCGTTTGTGAAGTCTCCGCCAATTTCTCTTGCAACAAGTACGCAAACAAAATACATACTTTTTTTTTTTTTTAATAATAATTGTCTAATAGGTCCATGAACGTTAATTTTGTGTCGAAAAAAACTTGACTGTCGTTATATAGGAATTTTTCTACATACCCACCATTATAATATTGACAACTAGGAGAGATAAAAAAAAGTTTTCTCATTTTCTACATTTATCTTTTAGAGTTGTTAAGATTTTAATGGGTTATGTAATAAGATCCCTACATACTAGCTAAGTTTTGTTTCGTGTGTCTAGTAGATCCTTGACATGTTCAAAATTTTTAAAAATTAACCAATCTATTTGATATAAAATTAAATTTGTTGGGAGTGCGACCAAAGTCCCACATTGGCTATGATAAGGGGATGATCATGGATTTATAAAGGATGACAAGTATCTCCATTGGTATGAGACATTTTGGGGTGAACCAAAAGCAGAGCCATGAGGGTTTATGCCCAAAATGGACAATATCTTATCATTGTGGAGATATGTGTTGTGTCTGTTGTCCCTTACAAAATTTTTGATTTATTAGAAACAAAATTGAAAGTTTTAGAACTCAAAGACTAAATTTGTAATTTAACTTTTGATGGTTTGCAGTAAAATCATCGGAGCTCGATCATATCGCATCAACGGTCAACTTCCTCCAACTGATATCCCAGGTCCGAGAGACTCAGACGGCCACGGGACACACACGGCATCGACAGTGGCCGGAGGATTGGTGAGGCAAGCGAACATGTTGGGTCTCGGCCTCGGCACGGCGAGGGGCGGAGTCCCATCGGCTCGTATTGCTGCTTACAAGGTATGCTGGTCGGACAGTTGCGATGACGCAGACATTCTCGCCGCATACGACGACGCCATTGCCGACGGAGTCGATATCCTTTCGGTCTCTATTGGAGGAGGAGAGGTGAGTGATTACTTCAACGACAGCATGGCCATTGGGGCTTTCCATGCAATGAGGAAGGGAATCCTCACGTCAATATCTGCCGGAAACGAGGGCCCTAACTTTTCAACTCTCACAAACTTCTCGCCGTGGTCGTTGACGGTGGCGGCCAGCACCACTGATCGGAAATTTGTCACAAAAGTTGAGCTCGGAGACGGACGAGGATTCGATGTAAGAAAAAGAAAATAATCTCATGTCGATCATTTTATTAAATAATTAAGGTTTCGTTTGATGACTATTTGGTTTTTAGTTTTTGGATTTTGAAAATTAAGTCTATACGACACACATACTTCTACCTATTAAGTTTCTTGGTTTTAGTATCAATTTTCTGCATTGTTTTCAAAAATCAAGTCCAATTTTGGAAAAAAAAAAATAGTTTTCAAAAACTTGTTTTTGTTTTTAAATTTTGGCTAAGATTTCAAATGCAACTTTAACTAGGATAGAAGTCATGTAGGAAATCTAGGAGAAAACAAACATAAATTTCAAAAACTAAAAACCAAATGGTTATCAAACGAGACTTTGGTTTTTGAAAATTAAGTTTATACACACATACTTCCACCTATAAGTTTCTTGGTTTTAGTATCAATCTTCTACCTTGTTTTCAAAAACCAAGTCTAATTTTGGAAAAAAAATAGTTTTCAAAAACTTGTTTTTGTTTTTAAAATTTAATTTTGGCTAGGAATTCAAATGCAATCTTAACTAGGATGAAAGTTGGAATTTAGGAGAAAAACAAGTATAATTTTCAAAAATAAAAAATAAAAAACCAAATGGTTATCAAACGGAGCTAATTAATTTAGATTTTTCTATAAATATATATAATTTTTATTTTGTTTTTGAAAGGGAGTAAGTGTCAATACATTTGATCTAAATGGAACACGATTTCCGTTGGTTTATGCCGGAGATATTCCTGCTGCTGGCTTCAACAAATCTGGTTCCAGGTAATTTTAAAATTACAATATGGGGTTGTTTTTTTTTGGGAATCTAGATGATCGGAATATTCAGATGCCTGGAATTATAGATACCCGGAAATATAGATGCCCGACATTTTAGATAACTGTGTTTGGTTTGAAGGAATAATTTATAAGAATATATGGGAATATTCAAATAACTATGTTTGTTTTGCAGAAGATCTTAGATTATGTATAATACCAATAATGTCCTTACTAATTTTTAGCTAATTTAGCTATTAGTTTAATATAGTTTAGTATTATTATTAAGAGAATAAATTAAAAATAAATTAAAATATCATTCAATATAACTATCATTTATTTTACATCTTAATGTAACATTTAAAAACAAATGGTTATTTCTTAGTGTATGAATAAATTAATTTATTTTTAAATAAAAATACAAATTGTAAAGTGGAGGGGTAAAAGTATCATCCGAAGATGCCCTCAACATGGGAATCTTTGAAACCCATCTCTCACGAGGGCATCAAAAGCTCCTCGGATGCCTCTCGTATGGACATCCTAGGATGCTCGGGCATCCCAAATTTCCTAGGTATCCAACATTACCACAAAACAAATGAGGTAATCCAGATGTCCATTCCATGGACATCTTGAATTACCGTCAAACAAACGGCCCCTATAGGGATGCAAGTGTAGATTAAAAAAATGATGAATAATTATATTTTGGTATGTTTAATTTTATAAAAAATAAAACAGAAAACGTTAAATTATAAACAATATGGTTGGTTGCAATTATACTTGTCAACTTTCAAAAGTTCCATTTTAGTTCTCAAAGTTTGAAATTTGTTTCAAAATGACCCTCAAAAACATTTTCTGTTAAATTTATGGACGAAAAATGAGGTGTTTGCATGTATTCTTATTGTTTTTTCCACGTATATGTTCAAATATATACACACAAGCAACAATATCCAATACAAACACATCGTTTTCTGTCTATAAATTTAACAAAAAAAAAAGGTTTCAAGGATTTTTTTGAAACAAAATTCAAACTTTAAAGACTAAAATGAAACTTTTAAAGGTATAATTGAAACGAACACAGAGTTGAGAGACATTTTTTTTTATACAATTAGTTTGTTTTTAATGGCAGGTAGGGTGACATCAGTCACTTTTTAATTGCTTTGTCTTGAATTGATTTTGGCAATTTACAGTTTTATCCTTTTTCAATTGCTCTGTCTTGAAATTGATTTTATCAATTTATAGTTTTAAGTTTACCATTATTTTTCGTTGATTTTTATTCCACAGTCAACTAGTTGTTTGACATCCAATAAGAGCATGACTCAACTAGCATAAAGCTTATATCTTGATTAAGAGGTCAGAGGTTCAATCCCCCACCTCTACATGTTGTCAAAAAAAAAAAAAAAAAAAAAAACTAGTTGTTTGATAGGGGATGTTAAGGAACGGGGATTTTCGACGAAAAATTTTTGGGATCGAGTTCTTCGCAGAGAATTATATTCTTTCTTTCTTTCTTTTAATCATTTAATAAAATTTTCAATTAGATAGTTAACTTTTCAGTTTTCAGTTAGATAGTTAATTATTAATCTTCATTTAAATACTTAATTTCTAAAAAAAATCTCTATTTAAATATAAAATTCTTAAACATCATAATAGAAATAAAATAATATTTCTAAAGGACCAGGGAGCGAGAATGAGATGCATTCTCCGCATTCTCCACCTCGTTAAGGACGGGTCCCCAACCAACAAGAGTTTGGTTTAATTGGCATAAGGCTTATATCTCGATCAAGAGGTCGAAGGTTCAATCTCCCACTCCTACATGTTGTCAAAAAAAAAAAAAAAAGATGGGTCCCCACGCGACCTCATCTTCAGGGGCAAATGAACATGATCTCTAGCCGTTGATTGTCAACGTTGGAAGAAATAGAATATATAAACATATAACTCCTTTTTTTTCCTGAATATATCAATTTACCAAAATGTCGAGTGAAGTTGTAATTCCAACAACTCCAATATTATATCATGCAATAAGTTGATGACTTATGGCAAATTACTTATTATATTAGGGAGAAAAATTATTTTACAAGAGTTTGAACACAAGACTTCCTGTTCTGACATCATGTTAATTAAATCACCCATCAAATCCAAAAACTTAAGCCGATGAGTTACAGTAACTTAAATTATAATTATATCAATGGTTTAACATTATTGACTAAAATAATATTGATATTGATTTATGATAGGCTTTGCATAGAGAACTCAGTGGACATGGAGTTGGTGAAGGGCAAAATCGCTCTATGTGATTTGTATCAAGGTCCAACACAAATAATAGGGGCTGTAAGAGGTGCAGCTGGTATTATAATGAGAGATGATTCTGCACTAGACCATACTTTCTCTTATCCATTGCCTGCTTCTCACCTTAATTCAAAAGATGGTGCTCTCATTTTCTCTTATATCGCCTCTAATCGTAGGTACGTTAATCCAATTTGTTTAGTTCAACTCATCGAGACATTAGAAATGATATGTCCGACACATGATTAAAATAGTTTAGTCCATGAATTTTCAAGTTTGTGTCTGTTTTGGTCGTTGGACGATCTGTTCGATACATGATTAAAATAGTTTAGTCCATGAATTTTCAAGTTTGTGTCTATTTTGGTAGTTGGACTTTGAAACATGTCTAGTAAGTCTTTGAACTTTCAGGATTGTGTCTATTTGGTTTTTGGACTCTAAAAAAATGGCAAAATACACTTTTGGTCCCTAAGTTTGAAAAATAGTTTTAAATGGTCCCTGAGTTAATTTGACCGTTAGTTGACTAACGAAAAAATGACTTGACACGTTGAGATGACAAATTTTAAAACAGGTGGACAAATTTTATTATTTTATATTAATTTTTTAGATTACACGGCTTTTATCCTTTATTTTTTCAACCAAATCACCATCTTCGTCCCCCATTTTCTCTCTCTAAAATGTCCATTCCCCCAAATCTCTCTCTTCCACTCTCCTCCCAAAACTATTAATTTAACGCTATGATACGATAGACAAATATGATATCAATTTTCTTGATTATTTTTGCATTACAATTAGGAAGGCATTTTATATACATTTTTTAATTGTGAATTTACTGATATAAAAGGAAAATATACCGCTATAAAATTTACAATTAAAGGAACAAATTATCCTAAGAATGATGGGAAGAATATACTCCAATATATTCAATTTAAAAAAAAAAATTCTGCAACTCTTATAGAGAGAGAAAGAGGGAGATAGAAGTAATTTTCTGTTTATAAACTTTTTTTTCAGAACTTTTGCAACTTAGAGAGGGAAAGAGAGAGATAGAAGTAATTTTTTGTTTATGCAACTTTTTTCTTTTGTAAATTTTTAGAGAGAGAAAGAGGGAGGTGAAACTATTTTTTTGTTCATGCAATTTCTTTTTTTTGTTTTTCTGCAGCTTTTTAGAGAGAGAAAGAGGAAGTTGAAACTAATTTTTTGTTTATTCAACTTCTTTTTTGGTTTTTCTGCAACTTTTTAGAGAGAGAAAGAGGGAGGTGAAACTAATTTTTTGTTCATGCAACTTCTTTTCTGGTTTTTCTGCGACTTTTTAGAGAGAGAAAATTAATTCCCTATCCCTCTTTCTCTCTCTAAAAATTGCAAAATAGAATTTTCTCCTAGTTGTAGAAATACACTTTTTCTTTTTTCTCTAAAAGAGTTGTTGAAATATAAACTTTCATTTTCCTTTTGCTTTATGGATTTTCTCTATCTAAAAAAGTGAAAAATAGAAGAAAATAAGAGAGGAGGAAGATAGGATAAGACTATGGATGAGAAAAAAAACTAGAGAGAAAATGAAGAAGAAAGAATTAAAAGAGGTGAGAATAGAGAAAGAATATCCCACAATAGGTGCCAACAAGAATATAAACATAAAGTTGTACTCATAACCACACCACGAGGTCTTGGTTCAAATTTTCACTCTCATATTGTCAAATATATATATATATATATATATATATATATATATATCACAATAGGTTAAAAATAATATAGAATTAATAGTTTTGGAGAGGAGAGAGAGAAATTTGGGGGAATGGACATTTTAGAGAGAGAACAAAAATGGGAGATGAAGATGGGAATTTGATGAAGGAAAAAGAAATGAGAAAAGCCATGTAATCTAAAAAATTAATATAAAATAATAAAATTTGTCCACATCATTTAAATTTTGTCGCCACGTCATTTTTCCGTTAGTCAACTAACGGTCAAATTAGTTCAGGGATCTATTTTCTATACCTGAACTAAAATGTATCTTTTGAAACCTCGGAGATCAAATAGACTATCAATCTCAAATCTCATGGAGCAAAAGTGTATTTTGCCTTAAAAATGTATAATGAAGACTTTGATATTTTGTTTTCAAGTTCATGGGTTTAAATTTATTGAACTTTCCCCACATGTAAATTAGTTTGTGGATTAACTAATTTCATGATTTATAGCATTCCAACTACAACTATATTGAAGAGCATTGAAGGAAAACATGAAAAAACTCCTTTTGTTGCAATTTTTTCTTCAAGGGGTCCAAACCCAATAACACCCAACATTCTCAAGGTATATAATTAAGTTCAATATTTCATAGTAATAAATTGATTTTTGAGTCGTTTTCTAATTTGTTTGTTTCTTGTGGATTTGTAAAGCCGGATTTGACTGGTCCTGGAGTTGAAATTCTTGCAGCGTGGTCTCCAATAGCTTCACCCTCAGGAGCTGAAGATGATACTAGAATGGTTCATTACAATATCATTTCAGGAACTTCTATGTCTTGCCCACATGCCACAGCAGCAGCTGCATATGTTAAATCGTTTCATCCTTCTTGGTCTCCTGCTGCCCTCAAATCAGCACTTATGACTACTGGTACTAAATGAAAATTTGATGATAGCTTAACACTTTCAAAGTTGCAACTTTACATAAAATTTCTTCATGTTGAAATTAAATCGATCTAAATTTATGCAGCATTTCCAATGAGCCTTGAAATTAACCCAAGTAGAGAATTTGCATATGGCTCTGGACATATAAATCCACTAGGCGCAGTGAATCCTGGATTAATCTACAATGCTACCGAGATCGACTATATAAAGTTCCTATGTGGCCAAGGTTATAGCACTGAGATGCTCCAACAAGTTTCTGGGATGATAGTACTTGTACCCCAGACAATTCCAACACAGTTTTTGACCTAAACTATCCTTCGTTTGCTCTTTCCACAATATCTCAATACCCATCAGCCAAATTTACAAAAGAAATGTCACAAATGTTGGGTCAACAAGTTCCACATATGAAGCCACCATTCTTAATCTTCCACAGGATCTTAATATTACAGTAAAGCCTTCTATTCTTTCATTCAAGGCATTGGGAGAGGAACAAAGCTTTGAGCTTAAAATTGAGGGGAAAATTAATTCTACCTTTGTATCAGCTTCCTTGTTGTGGGATGATGGTGAACACCAAGTGATAAGTCCTATAGTAGTGTTTGATGCTAATGCATTCACTAATTAG

mRNA sequence

ATGGACCAAGGAGGCAAAACCGGCAAATGGGACGGGCCAAGACCGAAGGGGTCGGGTTTTCGGCCCGACCCCCTGCTCGGCCTCGGCCATGGGCCGAGGCCGACCCTCGGCCATGGGCCGAGGCCGACCCTCGGCCCGCTCGTGCGGGCCGAGTCCGTTTGGTCTCGTCTGGTCCCCACCGCCTCTGGATGCCCCGGTTTCGCCTGGTTTGACCTAAAACGCCTCCGAAACCCTAAAAAGGCTAGGAGGATGAACAGGCCACGTCTTCCCCCCTCAACTACAAATTTATCGTTGGTGGCACGTGAAGGTCAGACATATATTGTATATATGGGAAGTCATTCAAAAAACAAAGTTTCAACTTCATTTCATCACATGAGAATACTAGAAGAAGCTATTGGCAGCAACTTTGCTCCACACTCTTTACTCCATAGCTACAAGAGAAGCTTCAATGGCTTTGTCGCAAAGTTGACTGAAGTTGAAGCACATAAAGTTTCTAAAATGAAAGGTGTAATCTCAATTTTTCCAAATGGAAAAAAGCAACTCCACACGACAAGGTCGTGGGACTTCATGGGTTTCTCCGAACAAGTCAACAGAGTCCCTACGGTGGAAAGCGACATCATTGTCGGAGTACTGGATACCGGCATTTGGCCGGAATCTCCAAGTTTCGATGACAAAGGTTACAGTCCGCCGCCAGCCAAGTGGAACGGCGTTTGTGAAGTCTCCGCCAATTTCTCTTGCAACAATAAAATCATCGGAGCTCGATCATATCGCATCAACGGTCAACTTCCTCCAACTGATATCCCAGGTCCGAGAGACTCAGACGGCCACGGGACACACACGGCATCGACAGTGGCCGGAGGATTGGTGAGGCAAGCGAACATGTTGGGTCTCGGCCTCGGCACGGCGAGGGGCGGAGTCCCATCGGCTCGTATTGCTGCTTACAAGGTATGCTGGTCGGACAGTTGCGATGACGCAGACATTCTCGCCGCATACGACGACGCCATTGCCGACGGAGTCGATATCCTTTCGGTCTCTATTGGAGGAGGAGAGGTGAGTGATTACTTCAACGACAGCATGGCCATTGGGGCTTTCCATGCAATGAGGAAGGGAATCCTCACGTCAATATCTGCCGGAAACGAGGGCCCTAACTTTTCAACTCTCACAAACTTCTCGCCGTGGTCGTTGACGGTGGCGGCCAGCACCACTGATCGGAAATTTGTCACAAAAGTTGAGCTCGGAGACGGACGAGGATTCGATGGAGTAAGTGTCAATACATTTGATCTAAATGGAACACGATTTCCGTTGGTTTATGCCGGAGATATTCCTGCTGCTGGCTTCAACAAATCTGGTTCCAGGCTTTGCATAGAGAACTCAGTGGACATGGAGTTGGTGAAGGGCAAAATCGCTCTATGTGATTTGTATCAAGGTCCAACACAAATAATAGGGGCTGTAAGAGGTGCAGCTGGTATTATAATGAGAGATGATTCTGCACTAGACCATACTTTCTCTTATCCATTGCCTGCTTCTCACCTTAATTCAAAAGATGGTGCTCTCATTTTCTCTTATATCGCCTCTAATCGTAGCATTCCAACTACAACTATATTGAAGAGCATTGAAGGAAAACATGAAAAAACTCCTTTTGTTGCAATTTTTTCTTCAAGGGGTCCAAACCCAATAACACCCAACATTCTCAAGCCGGATTTGACTGGTCCTGGAGTTGAAATTCTTGCAGCGTGGTCTCCAATAGCTTCACCCTCAGGAGCTGAAGATGATACTAGAATGGTTCATTACAATATCATTTCAGGAACTTCTATGTCTTGCCCACATGCCACAGCAGCAGCTGCATATGTTAAATCGTTTCATCCTTCTTGGTCTCCTGCTGCCCTCAAATCAGCACTTATGACTACTGCATTTCCAATGAGCCTTGAAATTAACCCAAGTAGAGAATTTGCATATGGCTCTGGACATATAAATCCACTAGGCGCAGTGAATCCTGGATTAATCTACAATGCTACCGAGATCGACTATATAAAGTTCCTATGTGGCCAAGGTTATAGCACTGAGATGCTCCAACAAGTTTCTGGGATGATAGATCTTAATATTACAGTAAAGCCTTCTATTCTTTCATTCAAGGCATTGGGAGAGGAACAAAGCTTTGAGCTTAAAATTGAGGGGAAAATTAATTCTACCTTTGTATCAGCTTCCTTGTTGTGGGATGATGGTGAACACCAAGTGATAAGTCCTATAGTAGTGTTTGATGCTAATGCATTCACTAATTAG

Coding sequence (CDS)

ATGGACCAAGGAGGCAAAACCGGCAAATGGGACGGGCCAAGACCGAAGGGGTCGGGTTTTCGGCCCGACCCCCTGCTCGGCCTCGGCCATGGGCCGAGGCCGACCCTCGGCCATGGGCCGAGGCCGACCCTCGGCCCGCTCGTGCGGGCCGAGTCCGTTTGGTCTCGTCTGGTCCCCACCGCCTCTGGATGCCCCGGTTTCGCCTGGTTTGACCTAAAACGCCTCCGAAACCCTAAAAAGGCTAGGAGGATGAACAGGCCACGTCTTCCCCCCTCAACTACAAATTTATCGTTGGTGGCACGTGAAGGTCAGACATATATTGTATATATGGGAAGTCATTCAAAAAACAAAGTTTCAACTTCATTTCATCACATGAGAATACTAGAAGAAGCTATTGGCAGCAACTTTGCTCCACACTCTTTACTCCATAGCTACAAGAGAAGCTTCAATGGCTTTGTCGCAAAGTTGACTGAAGTTGAAGCACATAAAGTTTCTAAAATGAAAGGTGTAATCTCAATTTTTCCAAATGGAAAAAAGCAACTCCACACGACAAGGTCGTGGGACTTCATGGGTTTCTCCGAACAAGTCAACAGAGTCCCTACGGTGGAAAGCGACATCATTGTCGGAGTACTGGATACCGGCATTTGGCCGGAATCTCCAAGTTTCGATGACAAAGGTTACAGTCCGCCGCCAGCCAAGTGGAACGGCGTTTGTGAAGTCTCCGCCAATTTCTCTTGCAACAATAAAATCATCGGAGCTCGATCATATCGCATCAACGGTCAACTTCCTCCAACTGATATCCCAGGTCCGAGAGACTCAGACGGCCACGGGACACACACGGCATCGACAGTGGCCGGAGGATTGGTGAGGCAAGCGAACATGTTGGGTCTCGGCCTCGGCACGGCGAGGGGCGGAGTCCCATCGGCTCGTATTGCTGCTTACAAGGTATGCTGGTCGGACAGTTGCGATGACGCAGACATTCTCGCCGCATACGACGACGCCATTGCCGACGGAGTCGATATCCTTTCGGTCTCTATTGGAGGAGGAGAGGTGAGTGATTACTTCAACGACAGCATGGCCATTGGGGCTTTCCATGCAATGAGGAAGGGAATCCTCACGTCAATATCTGCCGGAAACGAGGGCCCTAACTTTTCAACTCTCACAAACTTCTCGCCGTGGTCGTTGACGGTGGCGGCCAGCACCACTGATCGGAAATTTGTCACAAAAGTTGAGCTCGGAGACGGACGAGGATTCGATGGAGTAAGTGTCAATACATTTGATCTAAATGGAACACGATTTCCGTTGGTTTATGCCGGAGATATTCCTGCTGCTGGCTTCAACAAATCTGGTTCCAGGCTTTGCATAGAGAACTCAGTGGACATGGAGTTGGTGAAGGGCAAAATCGCTCTATGTGATTTGTATCAAGGTCCAACACAAATAATAGGGGCTGTAAGAGGTGCAGCTGGTATTATAATGAGAGATGATTCTGCACTAGACCATACTTTCTCTTATCCATTGCCTGCTTCTCACCTTAATTCAAAAGATGGTGCTCTCATTTTCTCTTATATCGCCTCTAATCGTAGCATTCCAACTACAACTATATTGAAGAGCATTGAAGGAAAACATGAAAAAACTCCTTTTGTTGCAATTTTTTCTTCAAGGGGTCCAAACCCAATAACACCCAACATTCTCAAGCCGGATTTGACTGGTCCTGGAGTTGAAATTCTTGCAGCGTGGTCTCCAATAGCTTCACCCTCAGGAGCTGAAGATGATACTAGAATGGTTCATTACAATATCATTTCAGGAACTTCTATGTCTTGCCCACATGCCACAGCAGCAGCTGCATATGTTAAATCGTTTCATCCTTCTTGGTCTCCTGCTGCCCTCAAATCAGCACTTATGACTACTGCATTTCCAATGAGCCTTGAAATTAACCCAAGTAGAGAATTTGCATATGGCTCTGGACATATAAATCCACTAGGCGCAGTGAATCCTGGATTAATCTACAATGCTACCGAGATCGACTATATAAAGTTCCTATGTGGCCAAGGTTATAGCACTGAGATGCTCCAACAAGTTTCTGGGATGATAGATCTTAATATTACAGTAAAGCCTTCTATTCTTTCATTCAAGGCATTGGGAGAGGAACAAAGCTTTGAGCTTAAAATTGAGGGGAAAATTAATTCTACCTTTGTATCAGCTTCCTTGTTGTGGGATGATGGTGAACACCAAGTGATAAGTCCTATAGTAGTGTTTGATGCTAATGCATTCACTAATTAG

Protein sequence

MDQGGKTGKWDGPRPKGSGFRPDPLLGLGHGPRPTLGHGPRPTLGPLVRAESVWSRLVPTASGCPGFAWFDLKRLRNPKKARRMNRPRLPPSTTNLSLVAREGQTYIVYMGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHKVSKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFDDKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTASTVAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILSVSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTDRKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSRLCIENSVDMELVKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFSYIASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSPIASPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMSLEINPSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQVSGMIDLNITVKPSILSFKALGEEQSFELKIEGKINSTFVSASLLWDDGEHQVISPIVVFDANAFTN
Homology
BLAST of Lag0040398 vs. NCBI nr
Match: XP_023550545.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 998.4 bits (2580), Expect = 3.3e-287
Identity = 497/711 (69.90%), Postives = 570/711 (80.17%), Query Frame = 0

Query: 104 QTYIVYMGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHK 163
           +TYIVYMGSH K++VST  HH+R+L+E IGS+FAPHSLLHSY+RSFNGFVAKLTEVE  K
Sbjct: 29  KTYIVYMGSHPKDRVSTRSHHVRMLQETIGSSFAPHSLLHSYERSFNGFVAKLTEVEVQK 88

Query: 164 VSKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFD 223
           VS+MKGVIS+FPNGKKQLHTTRSWDFMG S+Q +RVP+VESDIIVGVLDTGIWPESPSF 
Sbjct: 89  VSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQASRVPSVESDIIVGVLDTGIWPESPSFL 148

Query: 224 DKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTAST 283
           D+GY PPP KW G CEVS++FSCNNKIIGARSYR NGQ P  DI GPRDS+GHGTH AST
Sbjct: 149 DQGYGPPPRKWKGSCEVSSDFSCNNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHAAST 208

Query: 284 VAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILS 343
           VAGGLVRQA+MLGLG GTARGGVPSARIA+YK+CWSD C DAD+LAA+DDAIADGVDI+S
Sbjct: 209 VAGGLVRQASMLGLGSGTARGGVPSARIASYKICWSDGCRDADVLAAFDDAIADGVDIIS 268

Query: 344 VSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTD 403
            S+GG +  DYFNDS+AIGAFHAM+KGILTS+SAGN GP   T+ NFSPWSL+VAASTTD
Sbjct: 269 FSVGGRKPRDYFNDSIAIGAFHAMKKGILTSMSAGNSGPKSFTVRNFSPWSLSVAASTTD 328

Query: 404 RKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSRLCIENSVDMEL 463
           RKF++ V+LGDGR FDGV++NTFDLNGT++PLVYAG+IP  GFN S SR C+ NSVD EL
Sbjct: 329 RKFLSGVQLGDGRSFDGVTINTFDLNGTQYPLVYAGNIPNIGFNGSISRFCLTNSVDKEL 388

Query: 464 VKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFSYI 523
           VKGKI LCD +  PT  +  + GA GIIM+D++  D TF +PLPASHL +++GALI SY 
Sbjct: 389 VKGKIVLCDFFVSPTN-LSFLEGAIGIIMQDNNPKDLTFPFPLPASHLGTQEGALISSY- 448

Query: 524 ASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSPIA 583
           A+  S+PT TILKS EGK+++TPFVA FSSRGPNPITP+ILKPDL+GPGVEILAAWSPI 
Sbjct: 449 ANLTSLPTATILKSTEGKYKETPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWSPIG 508

Query: 584 SPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMSLE 643
            PSGAEDDTR + +NIISGTSMSCPHATA AAYVKSFHPSWSPAALKSALMTTAFPM  +
Sbjct: 509 PPSGAEDDTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAFPMRAD 568

Query: 644 INPSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQ----------- 703
           +NP  EFAYGSGHINPL AVNPGLIYNATEIDY++FLCGQGYST+++QQ           
Sbjct: 569 LNPDAEFAYGSGHINPLSAVNPGLIYNATEIDYVRFLCGQGYSTKLVQQVSGDNSSCSRG 628

Query: 704 --------------------------------------------VSGMIDLNITVKPSIL 760
                                                       VSG + LNITV PS+L
Sbjct: 629 DIDLVFDLNYPSFALSTSISTSISQVYRRRVTNVGSANSTYKAIVSGPLGLNITVNPSVL 688

BLAST of Lag0040398 vs. NCBI nr
Match: KAG6579374.1 (hypothetical protein SDJN03_23822, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 988.8 bits (2555), Expect = 2.6e-284
Identity = 496/711 (69.76%), Postives = 568/711 (79.89%), Query Frame = 0

Query: 104 QTYIVYMGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHK 163
           +TYIVYMGSH K +V T  HH+R+L+E IGS+FAPHSLLHSY+RSFNGFVAKLTEVE  K
Sbjct: 29  KTYIVYMGSHPKERVLTRSHHVRMLQETIGSSFAPHSLLHSYERSFNGFVAKLTEVEVQK 88

Query: 164 VSKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFD 223
           VS+MKGVIS+F NGKKQLHTTRSWDFMG S+Q +RVP+VESDIIVGVLDTGIWPESPSF 
Sbjct: 89  VSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQASRVPSVESDIIVGVLDTGIWPESPSFL 148

Query: 224 DKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTAST 283
           D+GY PPP KW G CEVS++FSCNNKIIGARSYR NGQ P  DI GPRDS+GHGTH AST
Sbjct: 149 DQGYGPPPPKWKGSCEVSSDFSCNNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHAAST 208

Query: 284 VAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILS 343
           VAG LVRQA+MLGLG GTARGGVPSARIA+YK+CWSD C DAD+LAA+DDAIADGVDI+S
Sbjct: 209 VAGELVRQASMLGLGSGTARGGVPSARIASYKICWSDGCRDADVLAAFDDAIADGVDIIS 268

Query: 344 VSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTD 403
            S+GG +  DYFNDS+AIGAFHAM+KGILTS+SAGN GP   T+ NFSPWSL+VAASTTD
Sbjct: 269 FSVGGRKPRDYFNDSIAIGAFHAMKKGILTSMSAGNSGPKSFTVRNFSPWSLSVAASTTD 328

Query: 404 RKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSRLCIENSVDMEL 463
           RK +T V+LGDGR FDGV+VNTFDLNGT++PLVYAG+IP  GFN S SR C+ NSVD E 
Sbjct: 329 RKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPLVYAGNIPNVGFNGSISRFCLTNSVDKES 388

Query: 464 VKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFSYI 523
           VKGKI LCD +  PT  +  + GA GIIM+D++  D TF +PLPASHL +++GALI SY 
Sbjct: 389 VKGKIVLCDFFISPTN-LSFLEGAIGIIMQDNNPKDLTFPFPLPASHLGTQEGALISSY- 448

Query: 524 ASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSPIA 583
           A+  S+PT TILKS EGK++ TPFVA FSSRGPNPITP+ILKPDL+GPGVEILAAWSPI 
Sbjct: 449 ANLTSLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWSPIG 508

Query: 584 SPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMSLE 643
            PSGAE+DTR + +NIISGTSMSCPHATA AAYVKSFHPSWSPAALKSALMTTAFPM  +
Sbjct: 509 PPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAFPMRSD 568

Query: 644 INPSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQVSG-------- 703
           +NP  EFAYGSGHINPLGAVNPGLIYNATEIDY++FLCGQGYST+++QQVSG        
Sbjct: 569 LNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRS 628

Query: 704 ----MIDLN-------------------------------------------ITVKPSIL 760
               + DLN                                           ITV PS+L
Sbjct: 629 DSDVVFDLNYPSFALSTPISTSISQVYRRKVTNVGSANSIYKATVSGPSGLKITVNPSVL 688

BLAST of Lag0040398 vs. NCBI nr
Match: XP_022922127.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 988.8 bits (2555), Expect = 2.6e-284
Identity = 495/711 (69.62%), Postives = 570/711 (80.17%), Query Frame = 0

Query: 104 QTYIVYMGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHK 163
           +TYIVYMGSH K++V T  HH+R+L+E IGS+FAPHSLLHSY+RSFNGFVAKLTEVE  K
Sbjct: 29  KTYIVYMGSHPKDRVLTRSHHVRMLQETIGSHFAPHSLLHSYERSFNGFVAKLTEVEVQK 88

Query: 164 VSKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFD 223
           VS+MKGVIS+FPNGKKQLHTTRSWDFMG S+Q +RVP+VESDIIVGVLDTGIWPESPSF 
Sbjct: 89  VSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQASRVPSVESDIIVGVLDTGIWPESPSFL 148

Query: 224 DKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTAST 283
           D+GY PPP KW G CEVS++FSCNNKIIGARSYR NGQ P  DI GPRDS+GHGTH AST
Sbjct: 149 DQGYGPPPRKWKGSCEVSSDFSCNNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHAAST 208

Query: 284 VAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILS 343
           VAGGLVRQA+MLGLG GTARGGVPSARIA+YK+CWSD C DADILAA+DDAIADGVDI+S
Sbjct: 209 VAGGLVRQASMLGLGSGTARGGVPSARIASYKICWSDGCPDADILAAFDDAIADGVDIIS 268

Query: 344 VSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTD 403
            S+GG    DYFNDS+AIGAFHAM+K ILTS+SAGN GP   T+ NFSPWSL+VAASTTD
Sbjct: 269 FSVGGQTPRDYFNDSIAIGAFHAMKKEILTSMSAGNNGPKSFTVRNFSPWSLSVAASTTD 328

Query: 404 RKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSRLCIENSVDMEL 463
           RKF+T V+LGDGR F+GV++NTFDLNGT++PLVYAG+IP  GFN S SR C+ NSVD E 
Sbjct: 329 RKFLTGVQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNIGFNGSISRFCLTNSVDKES 388

Query: 464 VKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFSYI 523
           VKGKI LCD +  PT  +  + GA GIIM+D++  D TF +PLPASHL +++GALI SY 
Sbjct: 389 VKGKIVLCDFFVLPTN-LNFLEGAIGIIMQDNNPKDLTFPFPLPASHLGTQEGALISSY- 448

Query: 524 ASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSPIA 583
           A+  S+PT TILKS EGK++ TPFVA FSSRGPNPITP+ILKPDL+GPGVEILAAWSPI 
Sbjct: 449 ANLTSLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWSPIG 508

Query: 584 SPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMSLE 643
            PSGAE+DTR + +NIISGTSMSCPHATA AAYVK+FHPSWSPAALKSALMTTAFPM  +
Sbjct: 509 PPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKTFHPSWSPAALKSALMTTAFPMRSD 568

Query: 644 INPSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQVSG-------- 703
           +NP  EFAYGSGHINPLGAVNPGLIYNATEIDY++FLCGQGYST+++QQVSG        
Sbjct: 569 LNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRS 628

Query: 704 ----MIDLN-------------------------------------------ITVKPSIL 760
               + DLN                                           ITV PS+L
Sbjct: 629 DSNVVFDLNYPSFALSTSISTPISQVYRRRVTNVGSANSTYNAIVSGPSRLKITVNPSVL 688

BLAST of Lag0040398 vs. NCBI nr
Match: XP_022922167.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 977.6 bits (2526), Expect = 6.0e-281
Identity = 490/713 (68.72%), Postives = 564/713 (79.10%), Query Frame = 0

Query: 104 QTYIVYMGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHK 163
           +TYIVYMGSH K++VST  HH+R+L+E IGS+FAPHSLLHSY+RSFNGFV KLTE E   
Sbjct: 29  RTYIVYMGSHPKDRVSTRSHHVRMLQETIGSSFAPHSLLHSYQRSFNGFVVKLTEDEVKI 88

Query: 164 VSKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFD 223
           +S+MKGVIS+FPN KKQLHTTRSWDFMG S+QV RVP+VESDIIVGVLDTGIWPESPSF 
Sbjct: 89  ISEMKGVISVFPNEKKQLHTTRSWDFMGLSQQVGRVPSVESDIIVGVLDTGIWPESPSFL 148

Query: 224 DKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTAST 283
           D+GY PPP +W G CE S NFSCNNKIIGARSYR NGQ P  DI GPRDS+GHGTHTAST
Sbjct: 149 DEGYGPPPPRWKGSCEASLNFSCNNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHTAST 208

Query: 284 VAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILS 343
           VAGGLVRQA+MLGLG GTARGGVPSARIA+YK+CWSD C DADILAA+DDAIADGVDI+S
Sbjct: 209 VAGGLVRQASMLGLGSGTARGGVPSARIASYKICWSDDCSDADILAAFDDAIADGVDIIS 268

Query: 344 VSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTD 403
            S+GG    DYFNDSMAIGAFHAM+KGILTS+SAGN+GP   T+ NFSPWSL+VAASTT+
Sbjct: 269 FSVGGHTPKDYFNDSMAIGAFHAMKKGILTSMSAGNDGPQSFTIRNFSPWSLSVAASTTN 328

Query: 404 RKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIP--AAGFNKSGSRLCIENSVDM 463
           R +++ ++LGDGR F+GV++NTFDLNGT++PLVYAG+IP    GFN S SR C+ NSVD 
Sbjct: 329 RSYLSGIQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNITGGFNGSISRFCLPNSVDR 388

Query: 464 ELVKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFS 523
           E VKGKI LCD +  P   +G++ GA GIIM+D +  D TF +PLPASHL +++GALI S
Sbjct: 389 ESVKGKIVLCDFFVSPKN-LGSLEGAIGIIMQDTNPKDLTFPFPLPASHLGTQEGALISS 448

Query: 524 YIASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSP 583
           Y A+   +PT TILKS EGK++ TPFVA FSSRGPNPITP+ILKPDL+GPGVEILAAWSP
Sbjct: 449 Y-ANLTGLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWSP 508

Query: 584 IASPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMS 643
           I  PSGAE+DTR + +NIISGTSMSCPHATA AAYVK+FHPSWSPAALKSALMTTAFPM 
Sbjct: 509 IGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKTFHPSWSPAALKSALMTTAFPMR 568

Query: 644 LEINPSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQVSG------ 703
            ++NP  EFAYGSGHINPL AVNPGLIYNATEIDY++FLCGQGYST+++QQVSG      
Sbjct: 569 ADLNPDAEFAYGSGHINPLSAVNPGLIYNATEIDYVRFLCGQGYSTKLVQQVSGDNSSCS 628

Query: 704 ------MID-------------------------------------------LNITVKPS 760
                 + D                                           LNITV PS
Sbjct: 629 RGDIDLVFDLNYPSFALSTSISTSISQVYRRRVTNVGSANSTYKAIVSGPSGLNITVNPS 688

BLAST of Lag0040398 vs. NCBI nr
Match: XP_038891432.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 975.3 bits (2520), Expect = 3.0e-280
Identity = 485/708 (68.50%), Postives = 563/708 (79.52%), Query Frame = 0

Query: 104 QTYIVYMGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHK 163
           +TYIVYMGSH K KVSTS HH+R+L+E IGS FAPHSLLHSY RSFNGFVAKLTE EA K
Sbjct: 31  KTYIVYMGSHPKGKVSTSSHHIRLLKETIGSTFAPHSLLHSYTRSFNGFVAKLTEPEAKK 90

Query: 164 VSKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFD 223
           VS+M+GVIS+FPNGKKQLHTTRSWDFMGFSEQV RVP VES++IVGVLD+GIWPESPSFD
Sbjct: 91  VSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFD 150

Query: 224 DKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTAST 283
             GY PPP KW G CEVSANFSCNNKIIG R+YR +G+ P  DI GPRDSDGHGTHTAS 
Sbjct: 151 HTGYGPPPPKWKGSCEVSANFSCNNKIIGGRAYRSDGKHPEGDIKGPRDSDGHGTHTASI 210

Query: 284 VAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILS 343
           VAGG+VRQA+MLGLGLGTARGGVPSARIA+YKVCWSD C DADILAA+DDAIADGVDI+S
Sbjct: 211 VAGGVVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCTDADILAAFDDAIADGVDIIS 270

Query: 344 VSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTD 403
            S+GG  + DYFNDS+AIG+FHAM+KGILTS++ GN GP+F+++ NFSPWSL+VAASTTD
Sbjct: 271 GSLGGSGIRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTD 330

Query: 404 RKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSRLCIENSVDMEL 463
           RKF TKVELGDGR F+GVS+NTFDL G + PLVYAGDIP A F+ S SR+C EN+V++EL
Sbjct: 331 RKFETKVELGDGREFNGVSINTFDLKGKQIPLVYAGDIPKAPFDSSVSRMCFENTVELEL 390

Query: 464 VKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFSYI 523
           VKGKI +CD    P  ++ A++GAAGIIM+DDS  D T S+P+PASHL  K GALI SYI
Sbjct: 391 VKGKIVVCDSLAVPGGVV-AIKGAAGIIMQDDSPQDDTNSFPIPASHLAPKPGALILSYI 450

Query: 524 ASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSPIA 583
            S  S+PT TI+KS E K ++ PFVA FSSRGPNPITPNILKPDLTGPGVEILAAW  IA
Sbjct: 451 NSTNSLPTATIMKSTERKRKRAPFVASFSSRGPNPITPNILKPDLTGPGVEILAAWPSIA 510

Query: 584 SPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMSLE 643
           SPSGAE+D++ V YNI+SGTSM+CPH TAAAAYVKSFHP+WSPAALKSAL+TTAF MSL+
Sbjct: 511 SPSGAEEDSKRVAYNILSGTSMACPHVTAAAAYVKSFHPTWSPAALKSALITTAFSMSLK 570

Query: 644 INPSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQVS--------- 703
            NP +EF YG+GHINPLGAV PGL+YNA+EIDY+KFLCGQGY+TE+LQ+VS         
Sbjct: 571 RNPDKEFGYGAGHINPLGAVQPGLVYNASEIDYVKFLCGQGYNTELLQRVSEDNDTCSSN 630

Query: 704 ---GMIDLN--------------------------------------------ITVKPSI 756
               + DLN                                            ITV PS+
Sbjct: 631 NSDTVFDLNYPSFALSTNISKSINQVYKRIVTNVGSKYATYKATIINPWKNLEITVNPSV 690

BLAST of Lag0040398 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 1.0e-214
Identity = 383/701 (54.64%), Postives = 475/701 (67.76%), Query Frame = 0

Query: 106 YIVYMGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHKVS 165
           YIVYMG   ++  S   HH  +LE+ +GS FAP S+LH+YKRSFNGF  KLTE EA K++
Sbjct: 34  YIVYMGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIA 93

Query: 166 KMKGVISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFDDK 225
            M+GV+S+F N   +LHTTRSWDF+GF   V R   VES+I+VGVLDTGIWPESPSFDD+
Sbjct: 94  SMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDE 153

Query: 226 GYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTASTVA 285
           G+SPPP KW G CE S NF CN KIIGARSY I   + P D+ GPRD++GHGTHTAST A
Sbjct: 154 GFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAA 213

Query: 286 GGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILSVS 345
           GGLV QAN+ GLGLGTARGGVP ARIAAYKVCW+D C D DILAAYDDAIADGVDI+S+S
Sbjct: 214 GGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLS 273

Query: 346 IGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTDRK 405
           +GG     YF D++AIG+FHA+ +GILTS SAGN GPNF T  + SPW L+VAAST DRK
Sbjct: 274 VGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRK 333

Query: 406 FVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSRLCIENSVDMELVK 465
           FVT+V++G+G+ F GVS+NTFD     +PLV   DIP  GF+KS SR C + SV+  L+K
Sbjct: 334 FVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLK 393

Query: 466 GKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFSYIAS 525
           GKI +C+   GP +   ++ GAAG++M  ++  D+  SYPLP+S L+  D      YI S
Sbjct: 394 GKIVVCEASFGPHEFFKSLDGAAGVLMTSNTR-DYADSYPLPSSVLDPNDLLATLRYIYS 453

Query: 526 NRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSPIASP 585
            RS P  TI KS    +   P V  FSSRGPN  T +++KPD++GPGVEILAAW  +A  
Sbjct: 454 IRS-PGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPV 513

Query: 586 SGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMSLEIN 645
            G   +T    +NIISGTSMSCPH T  A YVK+++P+WSPAA+KSALMTTA PM+   N
Sbjct: 514 GGIRRNTL---FNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFN 573

Query: 646 PSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQVSG---------- 705
           P  EFAYGSGH+NPL AV PGL+Y+A E DY+KFLCGQGY+T+ +++++G          
Sbjct: 574 PQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNT 633

Query: 706 --MIDLN-------------------------------------------ITVKPSILSF 752
             + DLN                                           I+V P++LSF
Sbjct: 634 GRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSF 693

BLAST of Lag0040398 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 1.1e-179
Identity = 336/705 (47.66%), Postives = 448/705 (63.55%), Query Frame = 0

Query: 106 YIVYMGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHKVS 165
           YIVYMG+  + K S   HH+ IL++ +G+  A H L+ SYKRSFNGF A L++ E+ K+ 
Sbjct: 33  YIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQ 92

Query: 166 KMKGVISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFDDK 225
            MK V+S+FP+   +L TTRSWDF+GF E+  R    ESD+IVGV+D+GIWPES SFDD+
Sbjct: 93  NMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDE 152

Query: 226 GYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTASTVA 285
           G+ PPP KW G C+    F+CNNK+IGAR Y              RD +GHGTHTAST A
Sbjct: 153 GFGPPPKKWKGSCKGGLKFACNNKLIGARFYN-------KFADSARDEEGHGTHTASTAA 212

Query: 286 GGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILSVS 345
           G  V+ A+  GL  GTARGGVPSARIAAYKVC+ + C+D DILAA+DDAIADGVD++S+S
Sbjct: 213 GNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISIS 272

Query: 346 IGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTDRK 405
           I    VS+  N S+AIG+FHAM +GI+T+ SAGN GP+  ++ N SPW +TVAAS TDR+
Sbjct: 273 ISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQ 332

Query: 406 FVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSRLCIENSVDMELVK 465
           F+ +V LG+G+   G+SVNTF+LNGT+FP+VY  ++ +   +++ +  C    VD ELVK
Sbjct: 333 FIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNV-SRNCSQAQAGYCSSGCVDSELVK 392

Query: 466 GKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFSYIAS 525
           GKI LCD + G  +    + GA G+I+++    D  F  P PAS L  +D   I SYI S
Sbjct: 393 GKIVLCDDFLGYRE--AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIES 452

Query: 526 NRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSPIASP 585
               P   IL++ E    + P+V  FSSRGP+ +  N+LKPD++ PG+EILAA+SP+ASP
Sbjct: 453 AEP-PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASP 512

Query: 586 SG--AEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMSLE 645
           S     +D R V Y+++SGTSM+CPH    AAYVKSFHP WSP+A+KSA+MTTA PM+L+
Sbjct: 513 SSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLK 572

Query: 646 INPSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQVSG-------- 705
            NP +EFAYGSG INP  A +PGL+Y     DY+K LC +G+ +  L   SG        
Sbjct: 573 KNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSER 632

Query: 706 ----------------------------------------------MIDLNITVKPSILS 753
                                                           +L I+++P IL 
Sbjct: 633 TEVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILR 692

BLAST of Lag0040398 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 3.1e-171
Identity = 335/727 (46.08%), Postives = 446/727 (61.35%), Query Frame = 0

Query: 94  TNLSLVAREGQTYIVYMGS-HSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGF 153
           +++S V  + Q YIVYMGS  S+   + +  HM IL+E  G +     L+ SYKRSFNGF
Sbjct: 20  SSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSFNGF 79

Query: 154 VAKLTEVEAHKVSKMKGVISIFPNGKKQLHTTRSWDFMGFSE--QVNRVPTVESDIIVGV 213
            A+LTE E  +V+KM GV+S+FPN K QL TT SWDFMG  E  +  R PTVESD I+GV
Sbjct: 80  AARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTIIGV 139

Query: 214 LDTGIWPESPSFDDKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGP 273
           +D+GI PES SF DKG+ PPP KW GVC    NF+CNNK+IGAR Y            G 
Sbjct: 140 IDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTSE---------GT 199

Query: 274 RDSDGHGTHTASTVAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAA 333
           RD DGHGTHTAST AG  V  A+  G+G GT RGGVP++R+AAYKVC    C    +L+A
Sbjct: 200 RDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEALLSA 259

Query: 334 YDDAIADGVDILSVSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNF 393
           +DDAIADGVD++++SIG    S + ND +AIGAFHAM KG+LT  SAGN GP   +++  
Sbjct: 260 FDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISVSGV 319

Query: 394 SPWSLTVAASTTDRKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSG 453
           +PW LTVAASTT+R FVTKV LG+G+   G SVN +++ G  +PLVY     ++  +   
Sbjct: 320 APWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACDAES 379

Query: 454 SRLCIENSVDMELVKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASH 513
           + LC  + VD   VKGKI +C    G  +I+ +V GA G+I R     D  F +PLPA+ 
Sbjct: 380 AGLCELSCVDKSRVKGKILVCG-GPGGLKIVESV-GAVGLIYRTPKP-DVAFIHPLPAAG 439

Query: 514 LNSKDGALIFSYIASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTG 573
           L ++D   + SY+ S  S P   +LK+    +  +P +A FSSRGPN I  +ILKPD+T 
Sbjct: 440 LLTEDFESLVSYLESTDS-PQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITA 499

Query: 574 PGVEILAAWSPIASPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALK 633
           PGVEILAA+SP   PS  +DDTR V Y+++SGTSMSCPH    AAYVK+F+P WSP+ ++
Sbjct: 500 PGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQ 559

Query: 634 SALMTTAFPMSLEIN--PSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTE 693
           SA+MTTA+P++       S EFAYGSGH++P+ A NPGL+Y   + D+I FLCG  Y+++
Sbjct: 560 SAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQ 619

Query: 694 MLQQVSGMI--------------------------------------------------- 753
           +L+ +SG                                                     
Sbjct: 620 VLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSK 679

Query: 754 -------DLNITVKPSILSFKALGEEQSFELKIEGKINSTFV--SASLLWDDGEHQVISP 756
                   L++ + PS+LSFK + E+QSF + + G    + V  SA+L+W DG H V SP
Sbjct: 680 VVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSP 731

BLAST of Lag0040398 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 1.4e-168
Identity = 325/715 (45.45%), Postives = 444/715 (62.10%), Query Frame = 0

Query: 104 QTYIVYMGS-HSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAH 163
           Q YIVY+GS  S+ + +    HM IL+E  G +   + L+ SYK+SFNGF A+LTE E  
Sbjct: 33  QVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKKSFNGFAARLTESERK 92

Query: 164 KVSKMKGVISIFPNGKKQLHTTRSWDFMGFSE--QVNRVPTVESDIIVGVLDTGIWPESP 223
           +++ M+ V+S+FP+ K +L TT SW+FMG  E  +  R  ++ESD I+GV+D+GI+PES 
Sbjct: 93  RLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESD 152

Query: 224 SFDDKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHT 283
           SF D+G+ PPP KW G C    NF+CNNK+IGAR Y    +   T     RD  GHGTHT
Sbjct: 153 SFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKSKANQT----ARDYSGHGTHT 212

Query: 284 ASTVAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVD 343
           AS  AG  V  +N  GLG GTARGGVP+ARIA YKVC ++ CD   +++A+DDAIADGVD
Sbjct: 213 ASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVD 272

Query: 344 ILSVSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAAS 403
           ++S+SI    +  +  D +AIGAFHAM  G+LT  +AGN GP  ST+T+ +PW  +VAAS
Sbjct: 273 VISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAAS 332

Query: 404 TTDRKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSRLCIENSVD 463
            T+R F+ KV LGDG+   G SVNT+D+NGT +PLVY      +  +   +RLC    +D
Sbjct: 333 VTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVDKARLCEPKCLD 392

Query: 464 MELVKGKIALCDLYQGPTQIIGAVR-GAAGIIMRDDSALDHTFSYPLPASHLNSKDGALI 523
            +LVKGKI LCD  +G   +I A + GA G I+++    D  F    P S L++ D   +
Sbjct: 393 GKLVKGKIVLCDSTKG---LIEAQKLGAVGSIVKNPEP-DRAFIRSFPVSFLSNDDYKSL 452

Query: 524 FSYIASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAW 583
            SY+ S ++ P  T+LKS E  +++ P VA FSSRGP+ I  +ILKPD+T PGVEILAA+
Sbjct: 453 VSYMNSTKN-PKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEILAAY 512

Query: 584 SPIASPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFP 643
           SP +SP+ +E DTR V Y+++SGTSM+CPH    AAYVK+FHP WSP+ ++SA+MTTA+P
Sbjct: 513 SPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWP 572

Query: 644 MSLEIN--PSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQ------ 703
           M+   +   S EFAYGSGH++P+ A+NPGL+Y  T+ D+I FLCG  Y+++ L+      
Sbjct: 573 MNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLNYTSDHLRIISGDN 632

Query: 704 ----------------------QVSGMIDLNIT--------------------------- 753
                                 +VSG    NIT                           
Sbjct: 633 STCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKL 692

BLAST of Lag0040398 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 5.1e-166
Identity = 327/722 (45.29%), Postives = 436/722 (60.39%), Query Frame = 0

Query: 97  SLVAREGQTYIVYMGSHSK--NKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVA 156
           +++  + Q YIVYMGS S   + + TS  HM IL++  G +     L+ SYKRSFNGF A
Sbjct: 24  AIIDEDTQVYIVYMGSLSSRADYIPTS-DHMSILQQVTGESSIEGRLVRSYKRSFNGFAA 83

Query: 157 KLTEVEAHKVSKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVN--RVPTVESDIIVGVLD 216
           +LTE E   +++++GV+S+FPN   QLHTT SWDFMG  E  N  R   +ESD I+GV+D
Sbjct: 84  RLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVID 143

Query: 217 TGIWPESPSFDDKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRD 276
           TGIWPES SF DKG+ PPP KW GVC    NF+CNNK+IGAR Y            G RD
Sbjct: 144 TGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSE---------GTRD 203

Query: 277 SDGHGTHTASTVAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYD 336
           + GHGTHTAST AG  V+  +  G+G GT RGGVP++RIAAYKVC    C    +L+++D
Sbjct: 204 TSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFD 263

Query: 337 DAIADGVDILSVSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSP 396
           DAIADGVD++++SIG    S + +D +AIGAFHAM KGILT  SAGN GP  +T+++ +P
Sbjct: 264 DAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAP 323

Query: 397 WSLTVAASTTDRKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSR 456
           W  TVAASTT+R F+TKV LG+G+   G SVN FD+ G ++PLVY     ++  +   + 
Sbjct: 324 WIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDAKTAA 383

Query: 457 LCIENSVDMELVKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLN 516
           LC    ++   VKGKI +C    GP+    A    A  I+      D  F++ LPAS L 
Sbjct: 384 LCAPACLNKSRVKGKILVCG---GPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLK 443

Query: 517 SKDGALIFSYIASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPG 576
           +KD   + SYI S  S P   +LK+    +  +P +A FSSRGPN I  +ILKPD+T PG
Sbjct: 444 AKDFKSLVSYIESQDS-PQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPG 503

Query: 577 VEILAAWSPIASPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSA 636
           VEILAA+SP   PS  EDDTR V Y++ SGTSM+CPH    AAYVK+F+P WSP+ ++SA
Sbjct: 504 VEILAAFSPNGEPS--EDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSA 563

Query: 637 LMTTAFPMSLEIN--PSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEML 696
           +MTTA+P+  +     S EFAYG+GH++P+ A+NPGL+Y   + D+I FLCG  Y+++ L
Sbjct: 564 IMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTL 623

Query: 697 QQVSGMI----------------------------------------------------- 753
           + +SG                                                       
Sbjct: 624 KIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVV 683

BLAST of Lag0040398 vs. ExPASy TrEMBL
Match: A0A6J1E2C5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430194 PE=3 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 1.3e-284
Identity = 495/711 (69.62%), Postives = 570/711 (80.17%), Query Frame = 0

Query: 104 QTYIVYMGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHK 163
           +TYIVYMGSH K++V T  HH+R+L+E IGS+FAPHSLLHSY+RSFNGFVAKLTEVE  K
Sbjct: 29  KTYIVYMGSHPKDRVLTRSHHVRMLQETIGSHFAPHSLLHSYERSFNGFVAKLTEVEVQK 88

Query: 164 VSKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFD 223
           VS+MKGVIS+FPNGKKQLHTTRSWDFMG S+Q +RVP+VESDIIVGVLDTGIWPESPSF 
Sbjct: 89  VSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQASRVPSVESDIIVGVLDTGIWPESPSFL 148

Query: 224 DKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTAST 283
           D+GY PPP KW G CEVS++FSCNNKIIGARSYR NGQ P  DI GPRDS+GHGTH AST
Sbjct: 149 DQGYGPPPRKWKGSCEVSSDFSCNNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHAAST 208

Query: 284 VAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILS 343
           VAGGLVRQA+MLGLG GTARGGVPSARIA+YK+CWSD C DADILAA+DDAIADGVDI+S
Sbjct: 209 VAGGLVRQASMLGLGSGTARGGVPSARIASYKICWSDGCPDADILAAFDDAIADGVDIIS 268

Query: 344 VSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTD 403
            S+GG    DYFNDS+AIGAFHAM+K ILTS+SAGN GP   T+ NFSPWSL+VAASTTD
Sbjct: 269 FSVGGQTPRDYFNDSIAIGAFHAMKKEILTSMSAGNNGPKSFTVRNFSPWSLSVAASTTD 328

Query: 404 RKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSRLCIENSVDMEL 463
           RKF+T V+LGDGR F+GV++NTFDLNGT++PLVYAG+IP  GFN S SR C+ NSVD E 
Sbjct: 329 RKFLTGVQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNIGFNGSISRFCLTNSVDKES 388

Query: 464 VKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFSYI 523
           VKGKI LCD +  PT  +  + GA GIIM+D++  D TF +PLPASHL +++GALI SY 
Sbjct: 389 VKGKIVLCDFFVLPTN-LNFLEGAIGIIMQDNNPKDLTFPFPLPASHLGTQEGALISSY- 448

Query: 524 ASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSPIA 583
           A+  S+PT TILKS EGK++ TPFVA FSSRGPNPITP+ILKPDL+GPGVEILAAWSPI 
Sbjct: 449 ANLTSLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWSPIG 508

Query: 584 SPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMSLE 643
            PSGAE+DTR + +NIISGTSMSCPHATA AAYVK+FHPSWSPAALKSALMTTAFPM  +
Sbjct: 509 PPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKTFHPSWSPAALKSALMTTAFPMRSD 568

Query: 644 INPSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQVSG-------- 703
           +NP  EFAYGSGHINPLGAVNPGLIYNATEIDY++FLCGQGYST+++QQVSG        
Sbjct: 569 LNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRS 628

Query: 704 ----MIDLN-------------------------------------------ITVKPSIL 760
               + DLN                                           ITV PS+L
Sbjct: 629 DSNVVFDLNYPSFALSTSISTPISQVYRRRVTNVGSANSTYNAIVSGPSRLKITVNPSVL 688

BLAST of Lag0040398 vs. ExPASy TrEMBL
Match: A0A6J1E2G4 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1)

HSP 1 Score: 977.6 bits (2526), Expect = 2.9e-281
Identity = 490/713 (68.72%), Postives = 564/713 (79.10%), Query Frame = 0

Query: 104 QTYIVYMGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHK 163
           +TYIVYMGSH K++VST  HH+R+L+E IGS+FAPHSLLHSY+RSFNGFV KLTE E   
Sbjct: 29  RTYIVYMGSHPKDRVSTRSHHVRMLQETIGSSFAPHSLLHSYQRSFNGFVVKLTEDEVKI 88

Query: 164 VSKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFD 223
           +S+MKGVIS+FPN KKQLHTTRSWDFMG S+QV RVP+VESDIIVGVLDTGIWPESPSF 
Sbjct: 89  ISEMKGVISVFPNEKKQLHTTRSWDFMGLSQQVGRVPSVESDIIVGVLDTGIWPESPSFL 148

Query: 224 DKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTAST 283
           D+GY PPP +W G CE S NFSCNNKIIGARSYR NGQ P  DI GPRDS+GHGTHTAST
Sbjct: 149 DEGYGPPPPRWKGSCEASLNFSCNNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHTAST 208

Query: 284 VAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILS 343
           VAGGLVRQA+MLGLG GTARGGVPSARIA+YK+CWSD C DADILAA+DDAIADGVDI+S
Sbjct: 209 VAGGLVRQASMLGLGSGTARGGVPSARIASYKICWSDDCSDADILAAFDDAIADGVDIIS 268

Query: 344 VSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTD 403
            S+GG    DYFNDSMAIGAFHAM+KGILTS+SAGN+GP   T+ NFSPWSL+VAASTT+
Sbjct: 269 FSVGGHTPKDYFNDSMAIGAFHAMKKGILTSMSAGNDGPQSFTIRNFSPWSLSVAASTTN 328

Query: 404 RKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIP--AAGFNKSGSRLCIENSVDM 463
           R +++ ++LGDGR F+GV++NTFDLNGT++PLVYAG+IP    GFN S SR C+ NSVD 
Sbjct: 329 RSYLSGIQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNITGGFNGSISRFCLPNSVDR 388

Query: 464 ELVKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFS 523
           E VKGKI LCD +  P   +G++ GA GIIM+D +  D TF +PLPASHL +++GALI S
Sbjct: 389 ESVKGKIVLCDFFVSPKN-LGSLEGAIGIIMQDTNPKDLTFPFPLPASHLGTQEGALISS 448

Query: 524 YIASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSP 583
           Y A+   +PT TILKS EGK++ TPFVA FSSRGPNPITP+ILKPDL+GPGVEILAAWSP
Sbjct: 449 Y-ANLTGLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWSP 508

Query: 584 IASPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMS 643
           I  PSGAE+DTR + +NIISGTSMSCPHATA AAYVK+FHPSWSPAALKSALMTTAFPM 
Sbjct: 509 IGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKTFHPSWSPAALKSALMTTAFPMR 568

Query: 644 LEINPSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQVSG------ 703
            ++NP  EFAYGSGHINPL AVNPGLIYNATEIDY++FLCGQGYST+++QQVSG      
Sbjct: 569 ADLNPDAEFAYGSGHINPLSAVNPGLIYNATEIDYVRFLCGQGYSTKLVQQVSGDNSSCS 628

Query: 704 ------MID-------------------------------------------LNITVKPS 760
                 + D                                           LNITV PS
Sbjct: 629 RGDIDLVFDLNYPSFALSTSISTSISQVYRRRVTNVGSANSTYKAIVSGPSGLNITVNPS 688

BLAST of Lag0040398 vs. ExPASy TrEMBL
Match: A0A0A0KWL9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G613590 PE=3 SV=1)

HSP 1 Score: 971.1 bits (2509), Expect = 2.7e-279
Identity = 488/708 (68.93%), Postives = 558/708 (78.81%), Query Frame = 0

Query: 104 QTYIVYMGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHK 163
           +TYIVYMGSHSK KVSTS HH+R+L+E IGS+F PHSLLHS+KRSFNGFVAKLTE E  K
Sbjct: 32  KTYIVYMGSHSKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEVKK 91

Query: 164 VSKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFD 223
           VS+M+GVIS+FPNGKKQLHTTRSWDFMGFSEQV RVP VES++IVGVLD+GIWPESPSFD
Sbjct: 92  VSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFD 151

Query: 224 DKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTAST 283
             GY  PPAKW G CEVSANFSCNNKIIGARSYR NG+ P  DI GPRDSDGHGTHTAS 
Sbjct: 152 HAGYGSPPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASI 211

Query: 284 VAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILS 343
           VAGGLVR+A+MLGLGLGTARGGVPSARIAAYKVCWSD C DADILAA+DDAIADGVDI+S
Sbjct: 212 VAGGLVRRASMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIIS 271

Query: 344 VSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTD 403
            S+GG    DYFNDS+AIG+FHAM+KGILTS++ GN GP+F+T+ NFSPWSL+VAASTTD
Sbjct: 272 GSLGGSGARDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTD 331

Query: 404 RKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSRLCIENSVDMEL 463
           RKF TKVELGDGR F GVSVNTFD+ G + PLVYAGDIP A F+ S SRLC EN+VD++L
Sbjct: 332 RKFETKVELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKL 391

Query: 464 VKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFSYI 523
           VKGKI +CD    P  ++ AV+GA GIIM+DDS+ D T S+P+PASHL  K GAL+ SYI
Sbjct: 392 VKGKIVVCDSLTVPGGVV-AVKGAVGIIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYI 451

Query: 524 ASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSPIA 583
            S  SIPT TI KS E K ++ P VA FSSRGPNPITPNILKPDL+GPGVEILAAWSP++
Sbjct: 452 NSTNSIPTATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPVS 511

Query: 584 SPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMSLE 643
            PSGAE+D + V YNIISGTSM+CPH TAAAAYVKSFHP+WSP+ALKSAL+TTAFPMS +
Sbjct: 512 PPSGAEEDNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPK 571

Query: 644 INPSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQVS--------- 703
            NP +EF YG+GHINPLGAV+PGLIY+A+EIDY++FLCGQGY+TE+LQ VS         
Sbjct: 572 HNPDKEFGYGAGHINPLGAVHPGLIYDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSN 631

Query: 704 ---GMIDLN--------------------------------------------ITVKPSI 756
               + DLN                                            I V PS+
Sbjct: 632 NSDTVFDLNYPSFALSTNISKPINQVYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSV 691

BLAST of Lag0040398 vs. ExPASy TrEMBL
Match: A0A6J1IAA2 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111471532 PE=3 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 1.3e-276
Identity = 489/710 (68.87%), Postives = 559/710 (78.73%), Query Frame = 0

Query: 105 TYIVYMGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHKV 164
           TYIVYMGSH K++VST  HH R+LEEAIGS+F   SLLHSYK+SFNGFV KLTE E  KV
Sbjct: 30  TYIVYMGSHPKDRVSTRSHHERMLEEAIGSSFTRGSLLHSYKKSFNGFVVKLTEDEVQKV 89

Query: 165 SKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFDD 224
           S+MKGVIS+FPNGKKQLHTTRSWDFMG S+QV+RVP VESDIIVGVLDTGIWPESPSF D
Sbjct: 90  SEMKGVISVFPNGKKQLHTTRSWDFMGLSQQVSRVPLVESDIIVGVLDTGIWPESPSFLD 149

Query: 225 KGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTASTV 284
           +GY PPP KW G CE S NFSCNNKIIGARSYR +G     DI GP DS+GHGTHTASTV
Sbjct: 150 EGYGPPPPKWKGSCETSLNFSCNNKIIGARSYRTSGNYAIGDIQGPIDSNGHGTHTASTV 209

Query: 285 AGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILSV 344
           AGGLVRQA+MLGLG GTARGGVPSARIA+YK+CWSD C DADILAA+DDAIADGVDI+S 
Sbjct: 210 AGGLVRQASMLGLGTGTARGGVPSARIASYKICWSDGCSDADILAAFDDAIADGVDIISF 269

Query: 345 SIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTDR 404
           S+GG   ++YFNDS+AIGAFHAM+ GILTS+SAGN+G    T+ NFSPWSL+VAASTTDR
Sbjct: 270 SVGGHTPNNYFNDSIAIGAFHAMKNGILTSMSAGNDGWKPFTIRNFSPWSLSVAASTTDR 329

Query: 405 KFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIP--AAGFNKSGSRLCIENSVDME 464
           +F++KV+LGDGR FDGV++NTFDLNGT++PLVYAG+IP  +AGFN S SR C+ NSVD +
Sbjct: 330 RFLSKVQLGDGRSFDGVTINTFDLNGTQYPLVYAGNIPNVSAGFNGSISRFCLRNSVDSD 389

Query: 465 LVKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFSY 524
           LVKGKI LCD +  P + I  + GA GIIM+D+   D TF +PLPASHL ++ GALI SY
Sbjct: 390 LVKGKIVLCDHFVSPKKTI-FLEGAIGIIMQDNRPKDLTFPFPLPASHLGTQQGALISSY 449

Query: 525 IASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSPI 584
            A+  S+PT TILKS EGK++ TPFVA FSSRGPNPITP+ILKPDL+GPGVEILAAW+P+
Sbjct: 450 -ANLTSLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWTPL 509

Query: 585 ASPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMSL 644
             PSGAE+DTR + +NIISGTSMSCPHATA AAYVKSFHPSWSPAALKSALMTTAFPM  
Sbjct: 510 GPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAFPMRA 569

Query: 645 EINPSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQVSG------- 704
           ++NP  EFAYGSGHINPLGAVNPGLIYNATEIDY++FLCGQGYST+++QQVSG       
Sbjct: 570 DLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKLVQQVSGDNSSCSR 629

Query: 705 -----MIDLN-------------------------------------------ITVKPSI 758
                + DLN                                           IT+ PS+
Sbjct: 630 GDFDLVFDLNYPSFALSTPMSTSISQVYRRRVTNVGSANSTYKAIVSGPSGLKITLNPSV 689

BLAST of Lag0040398 vs. ExPASy TrEMBL
Match: A0A6J1GYD4 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458626 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 3.7e-276
Identity = 488/709 (68.83%), Postives = 554/709 (78.14%), Query Frame = 0

Query: 104 QTYIVYMGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHK 163
           +TYIVYMGSH K KVSTS HH+R L E IGSNFAP SL+HSYKRSFNGFVAKLTE EA K
Sbjct: 30  KTYIVYMGSHPKGKVSTSSHHIRFLTETIGSNFAPRSLIHSYKRSFNGFVAKLTEAEAKK 89

Query: 164 VSKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFD 223
           VS+M+GVIS+FPNGKKQLHTTRSWDFMGFSEQV RVP VES+IIVGV D+GIWPESPSFD
Sbjct: 90  VSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNIIVGVFDSGIWPESPSFD 149

Query: 224 DKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTAST 283
           D GY PPPAKW G CEVSANFSCN+KIIGARSYR NG+    DI GP DSDGHGTHTAST
Sbjct: 150 DAGYGPPPAKWRGRCEVSANFSCNDKIIGARSYRSNGKHLENDIKGPIDSDGHGTHTAST 209

Query: 284 VAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILS 343
           VAGGLVRQA+MLGLGLGTARGGVPSARIAAYKVCWSD C DADILAA+DDAIADGVDI+S
Sbjct: 210 VAGGLVRQASMLGLGLGTARGGVPSARIAAYKVCWSDGCTDADILAAFDDAIADGVDIIS 269

Query: 344 VSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTD 403
            S+GG  V DYFNDS+AIG+FHAM+KGILT+++ GN GP F+T+ NFSPWSL+VAASTTD
Sbjct: 270 GSLGGSSVKDYFNDSIAIGSFHAMQKGILTTLAVGNNGPEFTTIVNFSPWSLSVAASTTD 329

Query: 404 RKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSRLCIENSVDMEL 463
           RKF TKV+LGDGR F+GVSVNTFDLNGT+ PLVYAGDIP A F++S SR+C EN+VD E 
Sbjct: 330 RKFETKVKLGDGREFNGVSVNTFDLNGTQIPLVYAGDIPKAPFDRSVSRMCFENTVDKER 389

Query: 464 VKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFSYI 523
           VKGKI +CD    P  ++ AV GAAGIIM+D+S  D T SYPLPASH+  K GALI SYI
Sbjct: 390 VKGKIVVCDSLAVPGGVV-AVEGAAGIIMQDESPQDDTNSYPLPASHVGPKPGALILSYI 449

Query: 524 ASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSPIA 583
            S R +PT +I+KS   K  + PFVA FSSRGPNPITPNILKPD++GPGVEILAAW    
Sbjct: 450 NSTR-VPTASIMKSRGRKRNRAPFVASFSSRGPNPITPNILKPDISGPGVEILAAWPSTV 509

Query: 584 SPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMSLE 643
           SPSGAE+D + V YN+ISGTSM+CPH TAAAAYVKSFHP+WSPAALKSAL+TTAFPMS +
Sbjct: 510 SPSGAEEDNKRVLYNVISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALITTAFPMSHK 569

Query: 644 INPSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQV---------- 703
            NP  E AYG+GHINPLGAV+PGLIYNA+E DY+KFLCGQGYSTE L+++          
Sbjct: 570 RNPDGELAYGAGHINPLGAVHPGLIYNASETDYVKFLCGQGYSTESLRRLSDDNASCSAN 629

Query: 704 -SGMI----------------------------------------------DLNITVKPS 756
            SG +                                              DL ITVKPS
Sbjct: 630 NSGTVFELNYPSFALSTNASNPISQVFRRRVTNVGSSYATSYKAVIVNPWRDLKITVKPS 689

BLAST of Lag0040398 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 623.2 bits (1606), Expect = 2.7e-178
Identity = 332/701 (47.36%), Postives = 444/701 (63.34%), Query Frame = 0

Query: 110 MGSHSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAHKVSKMKG 169
           MG+  + K S   HH+ IL++ +G+  A H L+ SYKRSFNGF A L++ E+ K+  MK 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 170 VISIFPNGKKQLHTTRSWDFMGFSEQVNRVPTVESDIIVGVLDTGIWPESPSFDDKGYSP 229
           V+S+FP+   +L TTRSWDF+GF E+  R    ESD+IVGV+D+GIWPES SFDD+G+ P
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 230 PPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHTASTVAGGLV 289
           PP KW G C+    F+CNNK+IGAR Y              RD +GHGTHTAST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYN-------KFADSARDEEGHGTHTASTAAGNAV 180

Query: 290 RQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVDILSVSIGGG 349
           + A+  GL  GTARGGVPSARIAAYKVC+ + C+D DILAA+DDAIADGVD++S+SI   
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 350 EVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAASTTDRKFVTK 409
            VS+  N S+AIG+FHAM +GI+T+ SAGN GP+  ++ N SPW +TVAAS TDR+F+ +
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 410 VELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSRLCIENSVDMELVKGKIA 469
           V LG+G+   G+SVNTF+LNGT+FP+VY  ++ +   +++ +  C    VD ELVKGKI 
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVYGQNV-SRNCSQAQAGYCSSGCVDSELVKGKIV 360

Query: 470 LCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLNSKDGALIFSYIASNRSI 529
           LCD + G  +    + GA G+I+++    D  F  P PAS L  +D   I SYI S    
Sbjct: 361 LCDDFLGYRE--AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP- 420

Query: 530 PTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAWSPIASPSG-- 589
           P   IL++ E    + P+V  FSSRGP+ +  N+LKPD++ PG+EILAA+SP+ASPS   
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480

Query: 590 AEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFPMSLEINPS 649
             +D R V Y+++SGTSM+CPH    AAYVKSFHP WSP+A+KSA+MTTA PM+L+ NP 
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540

Query: 650 REFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQVSG------------ 709
           +EFAYGSG INP  A +PGL+Y     DY+K LC +G+ +  L   SG            
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTEVK 600

Query: 710 ------------------------------------------MIDLNITVKPSILSFKAL 753
                                                       +L I+++P IL F  L
Sbjct: 601 DLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFL 660

BLAST of Lag0040398 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 603.6 bits (1555), Expect = 2.2e-172
Identity = 335/727 (46.08%), Postives = 446/727 (61.35%), Query Frame = 0

Query: 94  TNLSLVAREGQTYIVYMGS-HSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGF 153
           +++S V  + Q YIVYMGS  S+   + +  HM IL+E  G +     L+ SYKRSFNGF
Sbjct: 20  SSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSFNGF 79

Query: 154 VAKLTEVEAHKVSKMKGVISIFPNGKKQLHTTRSWDFMGFSE--QVNRVPTVESDIIVGV 213
            A+LTE E  +V+KM GV+S+FPN K QL TT SWDFMG  E  +  R PTVESD I+GV
Sbjct: 80  AARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTIIGV 139

Query: 214 LDTGIWPESPSFDDKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGP 273
           +D+GI PES SF DKG+ PPP KW GVC    NF+CNNK+IGAR Y            G 
Sbjct: 140 IDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTSE---------GT 199

Query: 274 RDSDGHGTHTASTVAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAA 333
           RD DGHGTHTAST AG  V  A+  G+G GT RGGVP++R+AAYKVC    C    +L+A
Sbjct: 200 RDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEALLSA 259

Query: 334 YDDAIADGVDILSVSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNF 393
           +DDAIADGVD++++SIG    S + ND +AIGAFHAM KG+LT  SAGN GP   +++  
Sbjct: 260 FDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISVSGV 319

Query: 394 SPWSLTVAASTTDRKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSG 453
           +PW LTVAASTT+R FVTKV LG+G+   G SVN +++ G  +PLVY     ++  +   
Sbjct: 320 APWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACDAES 379

Query: 454 SRLCIENSVDMELVKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASH 513
           + LC  + VD   VKGKI +C    G  +I+ +V GA G+I R     D  F +PLPA+ 
Sbjct: 380 AGLCELSCVDKSRVKGKILVCG-GPGGLKIVESV-GAVGLIYRTPKP-DVAFIHPLPAAG 439

Query: 514 LNSKDGALIFSYIASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTG 573
           L ++D   + SY+ S  S P   +LK+    +  +P +A FSSRGPN I  +ILKPD+T 
Sbjct: 440 LLTEDFESLVSYLESTDS-PQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITA 499

Query: 574 PGVEILAAWSPIASPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALK 633
           PGVEILAA+SP   PS  +DDTR V Y+++SGTSMSCPH    AAYVK+F+P WSP+ ++
Sbjct: 500 PGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQ 559

Query: 634 SALMTTAFPMSLEIN--PSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTE 693
           SA+MTTA+P++       S EFAYGSGH++P+ A NPGL+Y   + D+I FLCG  Y+++
Sbjct: 560 SAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQ 619

Query: 694 MLQQVSGMI--------------------------------------------------- 753
           +L+ +SG                                                     
Sbjct: 620 VLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSK 679

Query: 754 -------DLNITVKPSILSFKALGEEQSFELKIEGKINSTFV--SASLLWDDGEHQVISP 756
                   L++ + PS+LSFK + E+QSF + + G    + V  SA+L+W DG H V SP
Sbjct: 680 VVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSP 731

BLAST of Lag0040398 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 594.7 bits (1532), Expect = 1.0e-169
Identity = 325/715 (45.45%), Postives = 444/715 (62.10%), Query Frame = 0

Query: 104 QTYIVYMGS-HSKNKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVAKLTEVEAH 163
           Q YIVY+GS  S+ + +    HM IL+E  G +   + L+ SYK+SFNGF A+LTE E  
Sbjct: 33  QVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKKSFNGFAARLTESERK 92

Query: 164 KVSKMKGVISIFPNGKKQLHTTRSWDFMGFSE--QVNRVPTVESDIIVGVLDTGIWPESP 223
           +++ M+ V+S+FP+ K +L TT SW+FMG  E  +  R  ++ESD I+GV+D+GI+PES 
Sbjct: 93  RLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESD 152

Query: 224 SFDDKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRDSDGHGTHT 283
           SF D+G+ PPP KW G C    NF+CNNK+IGAR Y    +   T     RD  GHGTHT
Sbjct: 153 SFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKSKANQT----ARDYSGHGTHT 212

Query: 284 ASTVAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYDDAIADGVD 343
           AS  AG  V  +N  GLG GTARGGVP+ARIA YKVC ++ CD   +++A+DDAIADGVD
Sbjct: 213 ASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVD 272

Query: 344 ILSVSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSPWSLTVAAS 403
           ++S+SI    +  +  D +AIGAFHAM  G+LT  +AGN GP  ST+T+ +PW  +VAAS
Sbjct: 273 VISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAAS 332

Query: 404 TTDRKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSRLCIENSVD 463
            T+R F+ KV LGDG+   G SVNT+D+NGT +PLVY      +  +   +RLC    +D
Sbjct: 333 VTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVDKARLCEPKCLD 392

Query: 464 MELVKGKIALCDLYQGPTQIIGAVR-GAAGIIMRDDSALDHTFSYPLPASHLNSKDGALI 523
            +LVKGKI LCD  +G   +I A + GA G I+++    D  F    P S L++ D   +
Sbjct: 393 GKLVKGKIVLCDSTKG---LIEAQKLGAVGSIVKNPEP-DRAFIRSFPVSFLSNDDYKSL 452

Query: 524 FSYIASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPGVEILAAW 583
            SY+ S ++ P  T+LKS E  +++ P VA FSSRGP+ I  +ILKPD+T PGVEILAA+
Sbjct: 453 VSYMNSTKN-PKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEILAAY 512

Query: 584 SPIASPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSALMTTAFP 643
           SP +SP+ +E DTR V Y+++SGTSM+CPH    AAYVK+FHP WSP+ ++SA+MTTA+P
Sbjct: 513 SPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWP 572

Query: 644 MSLEIN--PSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQ------ 703
           M+   +   S EFAYGSGH++P+ A+NPGL+Y  T+ D+I FLCG  Y+++ L+      
Sbjct: 573 MNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLNYTSDHLRIISGDN 632

Query: 704 ----------------------QVSGMIDLNIT--------------------------- 753
                                 +VSG    NIT                           
Sbjct: 633 STCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKL 692

BLAST of Lag0040398 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 586.3 bits (1510), Expect = 3.6e-167
Identity = 327/722 (45.29%), Postives = 436/722 (60.39%), Query Frame = 0

Query: 97  SLVAREGQTYIVYMGSHSK--NKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVA 156
           +++  + Q YIVYMGS S   + + TS  HM IL++  G +     L+ SYKRSFNGF A
Sbjct: 24  AIIDEDTQVYIVYMGSLSSRADYIPTS-DHMSILQQVTGESSIEGRLVRSYKRSFNGFAA 83

Query: 157 KLTEVEAHKVSKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVN--RVPTVESDIIVGVLD 216
           +LTE E   +++++GV+S+FPN   QLHTT SWDFMG  E  N  R   +ESD I+GV+D
Sbjct: 84  RLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVID 143

Query: 217 TGIWPESPSFDDKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRD 276
           TGIWPES SF DKG+ PPP KW GVC    NF+CNNK+IGAR Y            G RD
Sbjct: 144 TGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSE---------GTRD 203

Query: 277 SDGHGTHTASTVAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYD 336
           + GHGTHTAST AG  V+  +  G+G GT RGGVP++RIAAYKVC    C    +L+++D
Sbjct: 204 TSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFD 263

Query: 337 DAIADGVDILSVSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSP 396
           DAIADGVD++++SIG    S + +D +AIGAFHAM KGILT  SAGN GP  +T+++ +P
Sbjct: 264 DAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAP 323

Query: 397 WSLTVAASTTDRKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSR 456
           W  TVAASTT+R F+TKV LG+G+   G SVN FD+ G ++PLVY     ++  +   + 
Sbjct: 324 WIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDAKTAA 383

Query: 457 LCIENSVDMELVKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLN 516
           LC    ++   VKGKI +C    GP+    A    A  I+      D  F++ LPAS L 
Sbjct: 384 LCAPACLNKSRVKGKILVCG---GPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLK 443

Query: 517 SKDGALIFSYIASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPG 576
           +KD   + SYI S  S P   +LK+    +  +P +A FSSRGPN I  +ILKPD+T PG
Sbjct: 444 AKDFKSLVSYIESQDS-PQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPG 503

Query: 577 VEILAAWSPIASPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSA 636
           VEILAA+SP   PS  EDDTR V Y++ SGTSM+CPH    AAYVK+F+P WSP+ ++SA
Sbjct: 504 VEILAAFSPNGEPS--EDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSA 563

Query: 637 LMTTAFPMSLEIN--PSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEML 696
           +MTTA+P+  +     S EFAYG+GH++P+ A+NPGL+Y   + D+I FLCG  Y+++ L
Sbjct: 564 IMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTL 623

Query: 697 QQVSGMI----------------------------------------------------- 753
           + +SG                                                       
Sbjct: 624 KIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVV 683

BLAST of Lag0040398 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 582.0 bits (1499), Expect = 6.8e-166
Identity = 326/720 (45.28%), Postives = 433/720 (60.14%), Query Frame = 0

Query: 97  SLVAREGQTYIVYMGSHSK--NKVSTSFHHMRILEEAIGSNFAPHSLLHSYKRSFNGFVA 156
           +++  + Q YIVYMGS S   + + TS  HM IL++  G +     L+ SYKRSFNGF A
Sbjct: 24  AIIDEDTQVYIVYMGSLSSRADYIPTS-DHMSILQQVTGESSIEGRLVRSYKRSFNGFAA 83

Query: 157 KLTEVEAHKVSKMKGVISIFPNGKKQLHTTRSWDFMGFSEQVN--RVPTVESDIIVGVLD 216
           +LTE E   +++++GV+S+FPN   QLHTT SWDFMG  E  N  R   +ESD I+GV+D
Sbjct: 84  RLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVID 143

Query: 217 TGIWPESPSFDDKGYSPPPAKWNGVCEVSANFSCNNKIIGARSYRINGQLPPTDIPGPRD 276
           TGIWPES SF DKG+ PPP KW GVC    NF+CNNK+IGAR Y            G RD
Sbjct: 144 TGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSE---------GTRD 203

Query: 277 SDGHGTHTASTVAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDSCDDADILAAYD 336
           + GHGTHTAST AG  V+  +  G+G GT RGGVP++RIAAYKVC    C    +L+++D
Sbjct: 204 TSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFD 263

Query: 337 DAIADGVDILSVSIGGGEVSDYFNDSMAIGAFHAMRKGILTSISAGNEGPNFSTLTNFSP 396
           DAIADGVD++++SIG    S + +D +AIGAFHAM KGILT  SAGN GP  +T+++ +P
Sbjct: 264 DAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAP 323

Query: 397 WSLTVAASTTDRKFVTKVELGDGRGFDGVSVNTFDLNGTRFPLVYAGDIPAAGFNKSGSR 456
           W  TVAASTT+R F+TKV LG+G+   G SVN FD+ G ++PLVY     ++  +   + 
Sbjct: 324 WIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDAKTAA 383

Query: 457 LCIENSVDMELVKGKIALCDLYQGPTQIIGAVRGAAGIIMRDDSALDHTFSYPLPASHLN 516
           LC    ++   VKGKI +C    GP+    A    A  I+      D  F++ LPAS L 
Sbjct: 384 LCAPACLNKSRVKGKILVCG---GPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLK 443

Query: 517 SKDGALIFSYIASNRSIPTTTILKSIEGKHEKTPFVAIFSSRGPNPITPNILKPDLTGPG 576
           +KD   + SYI S  S P   +LK+    +  +P +A FSSRGPN I  +ILKPD+T PG
Sbjct: 444 AKDFKSLVSYIESQDS-PQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPG 503

Query: 577 VEILAAWSPIASPSGAEDDTRMVHYNIISGTSMSCPHATAAAAYVKSFHPSWSPAALKSA 636
           VEILAA+SP   PS  EDDTR V Y++ SGTSM+CPH    AAYVK+F+P WSP+ ++SA
Sbjct: 504 VEILAAFSPNGEPS--EDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSA 563

Query: 637 LMTTAFPMSLEINPSREFAYGSGHINPLGAVNPGLIYNATEIDYIKFLCGQGYSTEMLQQ 696
           +MTTA    +    S EFAYG+GH++P+ A+NPGL+Y   + D+I FLCG  Y+++ L+ 
Sbjct: 564 IMTTAKGRGI---ASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKI 623

Query: 697 VSGMI------------------------------------------------------- 753
           +SG                                                         
Sbjct: 624 ISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAG 683

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023550545.13.3e-28769.90cucumisin-like [Cucurbita pepo subsp. pepo][more]
KAG6579374.12.6e-28469.76hypothetical protein SDJN03_23822, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022922127.12.6e-28469.62cucumisin-like [Cucurbita moschata][more]
XP_022922167.16.0e-28168.72cucumisin-like [Cucurbita moschata][more]
XP_038891432.13.0e-28068.50cucumisin-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q395471.0e-21454.64Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Q9FIF81.1e-17947.66Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... [more]
Q9FIG23.1e-17146.08Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... [more]
Q9FGU31.4e-16845.45Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... [more]
Q8L7D25.1e-16645.29Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1E2C51.3e-28469.62cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430194 PE=3 SV=1[more]
A0A6J1E2G42.9e-28168.72cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1[more]
A0A0A0KWL92.7e-27968.93Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G613590 PE=3 SV=1[more]
A0A6J1IAA21.3e-27668.87cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111471532 PE=3 SV=1[more]
A0A6J1GYD43.7e-27668.83cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458626 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G59190.12.7e-17847.36subtilase family protein [more]
AT5G59120.12.2e-17246.08subtilase 4.13 [more]
AT5G59100.11.0e-16945.45Subtilisin-like serine endopeptidase family protein [more]
AT5G59090.13.6e-16745.29subtilase 4.12 [more]
AT5G59090.26.8e-16645.28subtilase 4.12 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 601..617
score: 48.01
coord: 203..222
score: 32.38
coord: 272..285
score: 52.19
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 105..181
e-value: 4.1E-13
score: 49.8
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 695..751
e-value: 2.1E-10
score: 40.7
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 204..651
e-value: 2.7E-46
score: 158.1
NoneNo IPR availableGENE3D2.60.40.2310coord: 685..753
e-value: 1.5E-13
score: 52.6
NoneNo IPR availableGENE3D3.50.30.30coord: 403..541
e-value: 5.7E-186
score: 620.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..44
NoneNo IPR availablePANTHERPTHR10795:SF649PUTATIVE-RELATEDcoord: 93..695
coord: 699..753
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 186..663
score: 27.834415
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 410..534
e-value: 8.65108E-21
score: 86.6987
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 92..182
e-value: 1.7E-24
score: 88.0
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 205..676
e-value: 5.7E-186
score: 620.8
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 181..667
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 93..695
coord: 699..753
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 602..612
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 179..637
e-value: 1.10916E-127
score: 380.79

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040398.1Lag0040398.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity