Lag0040362 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0040362
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Heavy metal-associated isoprenylated plant protein 20-like
Location	chr13: 4190562 .. 4191647 (+)
RNA-Seq Expression	Lag0040362
Synteny	Lag0040362

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAATTTTTGACTCTGTCTCTGATTTAATCTCTGATTATGTGGCCACTTCCAGACAGAGGAAAAAAAAACCATTACAGGTTGCCTTCCCACCAATATTCTTCTCTTTCTCTCTTTTTTTTTTTTTTTCTTTACATTATGTTCTAAAATTCTATTTATTTCATTTTTCTGTTGATTTTACCCTACCAATTTTTATTATTTTTGTTTTAAGTTCAACTGTAAATTAGTATTAGAATCTCGAGGATATATGTTTTAACAAGTTAAACTATATTTAAGTTGGTTGGCTTGATTTTCTTTTTTATGTCGAGCTATACTTAAACTTGACAAGTTTGATTAGTTTTCACTTATATTCTAGCATATTAAATAAATCGATATCGAGTCGCCTTTACATTCTCTCGAATCAACAAACTCGAACAAGCTTTTGAAATTACTAACCAAAAATCATTTCTCTAAATACAAACGATTTAAAAACTTTTTAAAGTTGAACTAAATGTAAGATAGAAATTCAAATTCATTAAGCTAAAGACTTTTTTTAAAATATATATATATATATACATATTTTTTTAAAAAAAAAAACATTGATTTATTTATTTAGAGTTTTATTTTTGTGTCCTGTTTAAAGGGTTTTGATTTTGTTTGGGTGTAGACAGTGGAAATCAAGGTCAAAATGGACTGTGACGGCTGCGAACGAAGAGTAAAAAACGCCGTCTCCAAAATGAAAGGTCCGATCCCAAAACCCAAAAGATTTATTTATTTTCTCTAACAGATAAAATAATTTTTTTATTAAAACAGGTGCTAAAACGGTGGAGATAAACAGAAAACAGAGCAAAGTAACGGTAACGGGGTTCGTGGAGGCAAACAGAGTGTTGAAGAAAGTGAGAAGAACGGGAAAAAGAGCAGAGCTGTGGCCGTACGTTCCGTACAATGTGGTGGCGTATCCGTACGTGGCGCAGGCCTACGACAAGAGGGCGCCCGCCGGCTTCGTCAAAAACGCCGTTCAGGCCATTCCAAGCCCTAACGCCGTCGACGAGAAGCTCACCACTATGTTTAGCGACGACAATCCCAATGGCTGTTCCGTTATGTAA

mRNA sequence

ATGGGAATTTTTGACTCTGTCTCTGATTTAATCTCTGATTATGTGGCCACTTCCAGACAGAGGAAAAAAAAACCATTACAGACAGTGGAAATCAAGGTCAAAATGGACTGTGACGGCTGCGAACGAAGAGTAAAAAACGCCGTCTCCAAAATGAAAGGTGCTAAAACGGTGGAGATAAACAGAAAACAGAGCAAAGTAACGGTAACGGGGTTCGTGGAGGCAAACAGAGTGTTGAAGAAAGTGAGAAGAACGGGAAAAAGAGCAGAGCTGTGGCCGTACGTTCCGTACAATGTGGTGGCGTATCCGTACGTGGCGCAGGCCTACGACAAGAGGGCGCCCGCCGGCTTCGTCAAAAACGCCGTTCAGGCCATTCCAAGCCCTAACGCCGTCGACGAGAAGCTCACCACTATGTTTAGCGACGACAATCCCAATGGCTGTTCCGTTATGTAA

Coding sequence (CDS)

ATGGGAATTTTTGACTCTGTCTCTGATTTAATCTCTGATTATGTGGCCACTTCCAGACAGAGGAAAAAAAAACCATTACAGACAGTGGAAATCAAGGTCAAAATGGACTGTGACGGCTGCGAACGAAGAGTAAAAAACGCCGTCTCCAAAATGAAAGGTGCTAAAACGGTGGAGATAAACAGAAAACAGAGCAAAGTAACGGTAACGGGGTTCGTGGAGGCAAACAGAGTGTTGAAGAAAGTGAGAAGAACGGGAAAAAGAGCAGAGCTGTGGCCGTACGTTCCGTACAATGTGGTGGCGTATCCGTACGTGGCGCAGGCCTACGACAAGAGGGCGCCCGCCGGCTTCGTCAAAAACGCCGTTCAGGCCATTCCAAGCCCTAACGCCGTCGACGAGAAGCTCACCACTATGTTTAGCGACGACAATCCCAATGGCTGTTCCGTTATGTAA

Protein sequence

MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAVSKMKGAKTVEINRKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVKNAVQAIPSPNAVDEKLTTMFSDDNPNGCSVM

Homology

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BLAST of Lag0040362 vs. NCBI nr
Match: KAA0034616.1 (heavy metal-associated isoprenylated plant protein 20-like [Cucumis melo var. makuwa] >TYK09167.1 heavy metal-associated isoprenylated plant protein 20-like [Cucumis melo var. makuwa])

HSP 1 Score: 294.7 bits (753), Expect = 4.6e-76
Identity = 147/149 (98.66%), Postives = 149/149 (100.00%), Query Frame = 0


Query: 1   MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAVSKMKGAKTVEIN 60
           MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRV+NAVSKMKGAKTVE+N
Sbjct: 1   MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRVRNAVSKMKGAKTVEVN 60

Query: 61  RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVKNA 120
           RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVKNA
Sbjct: 61  RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVKNA 120

Query: 121 VQAIPSPNAVDEKLTTMFSDDNPNGCSVM 150
           VQAIPSPNAVDEKLTTMFSDDNPNGCSVM
Sbjct: 121 VQAIPSPNAVDEKLTTMFSDDNPNGCSVM 149

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BLAST of Lag0040362 vs. NCBI nr
Match: XP_038892702.1 (heavy metal-associated isoprenylated plant protein 20-like [Benincasa hispida])

HSP 1 Score: 294.3 bits (752), Expect = 6.0e-76
Identity = 147/149 (98.66%), Postives = 148/149 (99.33%), Query Frame = 0


Query: 1   MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAVSKMKGAKTVEIN 60
           MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAVSKMKGAKTVE+N
Sbjct: 1   MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAVSKMKGAKTVEVN 60

Query: 61  RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVKNA 120
           RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYV QAYDKRAPAGFVKNA
Sbjct: 61  RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVTQAYDKRAPAGFVKNA 120

Query: 121 VQAIPSPNAVDEKLTTMFSDDNPNGCSVM 150
           VQAIPSPNAVDEKLTTMFSDDNPNGCSVM
Sbjct: 121 VQAIPSPNAVDEKLTTMFSDDNPNGCSVM 149

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BLAST of Lag0040362 vs. NCBI nr
Match: XP_011655816.1 (heavy metal-associated isoprenylated plant protein 20 isoform X1 [Cucumis sativus] >KGN52144.1 hypothetical protein Csa_008954 [Cucumis sativus])

HSP 1 Score: 293.1 bits (749), Expect = 1.3e-75
Identity = 146/149 (97.99%), Postives = 148/149 (99.33%), Query Frame = 0


Query: 1   MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAVSKMKGAKTVEIN 60
           MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAV+KMKGAKTVE+N
Sbjct: 1   MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAVTKMKGAKTVEVN 60

Query: 61  RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVKNA 120
           RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYV QAYDKRAPAGFVKNA
Sbjct: 61  RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVTQAYDKRAPAGFVKNA 120

Query: 121 VQAIPSPNAVDEKLTTMFSDDNPNGCSVM 150
           VQAIPSPNAVDEKLTTMFSDDNPNGCSVM
Sbjct: 121 VQAIPSPNAVDEKLTTMFSDDNPNGCSVM 149

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BLAST of Lag0040362 vs. NCBI nr
Match: XP_016900217.1 (PREDICTED: LOW QUALITY PROTEIN: heavy metal-associated isoprenylated plant protein 20-like [Cucumis melo])

HSP 1 Score: 289.7 bits (740), Expect = 1.5e-74
Identity = 144/149 (96.64%), Postives = 148/149 (99.33%), Query Frame = 0


Query: 1   MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAVSKMKGAKTVEIN 60
           MGIFDSVSDLISDYVATSR ++KKPLQTVEIKVKMDCDGCERRV+NAVSKMKGAKTVE+N
Sbjct: 1   MGIFDSVSDLISDYVATSRXKEKKPLQTVEIKVKMDCDGCERRVRNAVSKMKGAKTVEVN 60

Query: 61  RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVKNA 120
           RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVKNA
Sbjct: 61  RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVKNA 120

Query: 121 VQAIPSPNAVDEKLTTMFSDDNPNGCSVM 150
           VQAIPSPNAVDEKLTTMFSDDNPNGCSVM
Sbjct: 121 VQAIPSPNAVDEKLTTMFSDDNPNGCSVM 149

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BLAST of Lag0040362 vs. NCBI nr
Match: XP_022150634.1 (heavy metal-associated isoprenylated plant protein 20 [Momordica charantia])

HSP 1 Score: 285.8 bits (730), Expect = 2.1e-73
Identity = 141/149 (94.63%), Postives = 147/149 (98.66%), Query Frame = 0


Query: 1   MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAVSKMKGAKTVEIN 60
           MGIFDSVSDLIS+YVATSRQRKKKP+QTVEIKVKMDCDGCERRV+NAV+KMKG KTVE+N
Sbjct: 1   MGIFDSVSDLISEYVATSRQRKKKPMQTVEIKVKMDCDGCERRVRNAVAKMKGVKTVEVN 60

Query: 61  RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVKNA 120
           RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVP NVVAYPYVAQAYDKRAPAGFVKNA
Sbjct: 61  RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPVNVVAYPYVAQAYDKRAPAGFVKNA 120

Query: 121 VQAIPSPNAVDEKLTTMFSDDNPNGCSVM 150
           VQA+PSPNAVDEKLTTMFSDDNPNGCSVM
Sbjct: 121 VQALPSPNAVDEKLTTMFSDDNPNGCSVM 149

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BLAST of Lag0040362 vs. ExPASy Swiss-Prot
Match: Q9C9A3 (Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana OX=3702 GN=HIPP20 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 3.6e-47
Identity = 92/152 (60.53%), Postives = 118/152 (77.63%), Query Frame = 0


Query: 1   MGIFDSVSDLISDYV-ATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAVSKMKGAKTVEI 60
           MG  DS+S+ ISDY   T ++RK+K +QTV IKVKMDCDGCERRVKNAVS MKG K+VE+
Sbjct: 1   MGALDSLSEYISDYFRVTRKRRKRKVMQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEV 60

Query: 61  NRKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVKN 120
           NRK  KVTV+G+VE  +VLK++ RTGK+AE+WPYVPYN+VAYPY    YDK+APAG+V+ 
Sbjct: 61  NRKIHKVTVSGYVEPKKVLKRIERTGKKAEIWPYVPYNMVAYPYAVGTYDKKAPAGYVRK 120

Query: 121 AVQAIPS--PNAVDEKLTTMFSDDNPNGCSVM 150
           + Q+     P A +    ++FSD+NPN C+VM
Sbjct: 121 SEQSQLQLLPGAPENHYISLFSDENPNACTVM 152

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BLAST of Lag0040362 vs. ExPASy Swiss-Prot
Match: Q93VP2 (Heavy metal-associated isoprenylated plant protein 22 OS=Arabidopsis thaliana OX=3702 GN=HIPP22 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 8.8e-46
Identity = 89/152 (58.55%), Postives = 120/152 (78.95%), Query Frame = 0


Query: 1   MGIFDSVSDLISD--YVATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAVSKMKGAKTVE 60
           MG  + +S+  S+  YV+  +++K+K +QTV IKVK+DCDGCER++KNAVS +KGAK+VE
Sbjct: 1   MGALNYLSEYFSNHFYVSIRKRKKRKVMQTVNIKVKIDCDGCERKIKNAVSSIKGAKSVE 60

Query: 61  INRKQSKVTVTGFVEANRVLKKVRRTG-KRAELWPYVPYNVVAYPYVAQAYDKRAPAGFV 120
           +NRK  KVTV+G+V+  +VLK V+ TG K+AELWPYVPY +VAYPY A AYDKRAP GFV
Sbjct: 61  VNRKMHKVTVSGYVDPKKVLKTVQSTGKKKAELWPYVPYTMVAYPYAAGAYDKRAPPGFV 120

Query: 121 KNAVQAIPSPNAVDEKLTTMFSDDNPNGCSVM 150
           + + QA   P + D+KL ++FSD+NPN C+VM
Sbjct: 121 RKSEQAQAQPGSTDDKLMSLFSDENPNACTVM 152

[Close]

BLAST of Lag0040362 vs. ExPASy Swiss-Prot
Match: Q9LF57 (Heavy metal-associated isoprenylated plant protein 21 OS=Arabidopsis thaliana OX=3702 GN=HIPP21 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 6.3e-44
Identity = 84/150 (56.00%), Postives = 113/150 (75.33%), Query Frame = 0


Query: 1   MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAVSKMKGAKTVEIN 60
           MG FD +S   S Y   + + K+KPLQTV+IKVKMDCDGCERRV+N V +MKG K+VE+N
Sbjct: 1   MGAFDYISSFCS-YTYANAKTKRKPLQTVDIKVKMDCDGCERRVRNVVRRMKGVKSVEVN 60

Query: 61  RKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVKNA 120
           RKQS++TV G V+ N+VLK+V+ TGK+AE WPY+P ++V YP+    YDKRAPAG ++N 
Sbjct: 61  RKQSRITVNGHVDPNKVLKRVKSTGKKAEFWPYIPQHMVYYPFAPGMYDKRAPAGHIRNP 120

Query: 121 VQAIPSPNAVDEKLTTMFSDDNPN-GCSVM 150
            Q+ P+ NA +E   ++FSDDN +  CS+M
Sbjct: 121 TQSFPTANAPEENYVSLFSDDNVHAACSIM 149

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BLAST of Lag0040362 vs. ExPASy Swiss-Prot
Match: O65657 (Heavy metal-associated isoprenylated plant protein 23 OS=Arabidopsis thaliana OX=3702 GN=HIPP23 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.3e-41
Identity = 82/154 (53.25%), Postives = 115/154 (74.68%), Query Frame = 0


Query: 2   GIFDSVSDLISDYVATS--RQRKKKPLQTVEIKVKMDCDGCERRVKNAVSKMKGAKTVEI 61
           G  + +S+LI +  + S  +++KKK  QTVE+KV+MDCDGC  ++KN++S +KG KTVEI
Sbjct: 5   GTLEYISELIGNGGSHSYGKRKKKKQFQTVELKVRMDCDGCVLKIKNSLSSLKGVKTVEI 64

Query: 62  NRKQSKVTVTGFVEANRVLKKVRRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVK- 121
           N+KQ KVTV+G+ +A++VLKK + TGK+AE+WPYVPYN+VA PY+AQAYDK+AP G+V+ 
Sbjct: 65  NKKQQKVTVSGYADASKVLKKAKATGKKAEIWPYVPYNLVAQPYIAQAYDKKAPPGYVRK 124

Query: 122 ---NAVQAIPSPNAVDEKLTTMFSDDNPNGCSVM 150
              N      +    D   T++FSDDNPN CS+M
Sbjct: 125 VDPNVTTGTMAVYYDDPSYTSLFSDDNPNACSIM 158

[Close]

BLAST of Lag0040362 vs. ExPASy Swiss-Prot
Match: Q9SZN7 (Heavy metal-associated isoprenylated plant protein 26 OS=Arabidopsis thaliana OX=3702 GN=HIPP26 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 9.1e-35
Identity = 75/154 (48.70%), Postives = 106/154 (68.83%), Query Frame = 0


Query: 1   MGIFDSVSDLISDYVATSRQRKKKPLQTVEIKVKMDCDGCERRVKNAVSKMKGAKTVEIN 60
           MG+ D VS++  D     + +K+K LQTVEIKVKMDC+GCER+V+ +V  MKG  +V + 
Sbjct: 1   MGVLDHVSEMF-DCSHGHKIKKRKQLQTVEIKVKMDCEGCERKVRRSVEGMKGVSSVTLE 60

Query: 61  RKQSKVTVTGFVEANRVLKKV-RRTGKRAELWPYVPYNVVAYPYVAQAYDKRAPAGFVKN 120
            K  KVTV G+V+ N+V+ ++  RTGK+ ELWPYVPY+VVA+PY A  YDK+AP+G+V+ 
Sbjct: 61  PKAHKVTVVGYVDPNKVVARMSHRTGKKVELWPYVPYDVVAHPYAAGVYDKKAPSGYVRR 120

Query: 121 ----AVQAIPSPNAVDEKLTTMFSDDNPNGCSVM 150
                V  +   ++ + + TT FSD+NP  C VM
Sbjct: 121 VDDPGVSQLARASSTEVRYTTAFSDENPAACVVM 153

The following BLAST results are available for this feature:

BLAST of Lag0040362 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0034616.1	4.6e-76	98.66	heavy metal-associated isoprenylated plant protein 20-like [Cucumis melo var. ma...	[more]
XP_038892702.1	6.0e-76	98.66	heavy metal-associated isoprenylated plant protein 20-like [Benincasa hispida]	[more]
XP_011655816.1	1.3e-75	97.99	heavy metal-associated isoprenylated plant protein 20 isoform X1 [Cucumis sativu...	[more]
XP_016900217.1	1.5e-74	96.64	PREDICTED: LOW QUALITY PROTEIN: heavy metal-associated isoprenylated plant prote...	[more]
XP_022150634.1	2.1e-73	94.63	heavy metal-associated isoprenylated plant protein 20 [Momordica charantia]	[more]

BLAST of Lag0040362 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C9A3	3.6e-47	60.53	Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana OX...	[more]
Q93VP2	8.8e-46	58.55	Heavy metal-associated isoprenylated plant protein 22 OS=Arabidopsis thaliana OX...	[more]
Q9LF57	6.3e-44	56.00	Heavy metal-associated isoprenylated plant protein 21 OS=Arabidopsis thaliana OX...	[more]
O65657	1.3e-41	53.25	Heavy metal-associated isoprenylated plant protein 23 OS=Arabidopsis thaliana OX...	[more]
Q9SZN7	9.1e-35	48.70	Heavy metal-associated isoprenylated plant protein 26 OS=Arabidopsis thaliana OX...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 148..149
None	No IPR available	GENE3D	3.30.70.100		coord: 13..92 e-value: 7.4E-19 score: 69.7
None	No IPR available	PANTHER	PTHR22814:SF301	HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 22-LIKE	coord: 2..149
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 2..149
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 32..86 e-value: 6.7E-13 score: 48.8
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 35..90 score: 11.429252
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 30..89 e-value: 1.07629E-12 score: 57.2305
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 26..92

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0040362.1	Lag0040362.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding