Lag0040309 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0040309
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like
Location	chr13: 3758106 .. 3760241 (-)
RNA-Seq Expression	Lag0040309
Synteny	Lag0040309

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGCAGGCTTCCATTAATCCGATCCAATCGCCGCCTCCGGTGTTCAATAACAACACTACGGGACCCCCAATTCCTTCATCAATTGCCTGGCCCATCAGCCGGCCGTCGACATCGTTACGCCTACGTTCGCCCCCAGTAACGAGAAGCTCCAACTCTCAAACATTTGATCTCAAATCGGGCAAAGGGTACTTCTAACTTTCAAAAAATTGTCAATGTCTTTTTCCTATTCAGCATTCTATCTTTGCCAAGATCTTGAACATTATGTTGTCTGTTATGGCTTCTGCTTGCTCGACATTAGCAGACCTTCGCGCATTATGTCCCCCCGCCCCCTCTCTCGAGGAAAATTTTGCCAATGATTTTAGATGAACGAGCTGACTTTTGTACTGATGCTCGGTTTGTTTATTTACTTGTGTTTGGAATTTGGGTGGAGTGTTGAATTTCTTTTATTTAATTGCATCGGGTTCTACAATTAAGAGGATGGATTCTTTTGATGTTTATCCTTTTGCGTTGCAATTGTTGTTTCTGTTTACATTATCCGGATGTAATGCCTTTCGTTTTGTTTTGGATAAAACGTCTTGACCTTATTGAAAAATGTAAAAGATTGAACAACTTGACGACTGTAGGATGGATGGCTTCCTAGATGTTGAGCTCAAAGTTCGTGATTATGAGCTGGATCAGTATGGTGTTGTGAACAATGCTGTCTATGCAAGTTATTGCCAACATGGTAAAATCAAACATCCACTTCTTCAATTGTGTTCCCTTTCAAATTTGTTTGCTCATTGTTCTTCTAGTGATATTTGTCCGAATTGGGTTGCATTTGTTTTTCCTCTCTTGGGTTTCTGTTTCTTTATGTTTTGTCTTTCACTCCCTCATTTTTGTACCTTTTCATTTCTTTAATGAGTTGTTTGTATCTTTTCAAGTGTGGAGCCTTTAACATTTCAAATGTTGGTGTTTGTTTATGTCCAATGGGTCCAGAATTTCTGTTTCACGAGCATTGATGTGACTGATTCACGCACTAATTAGCATTATATCTCATGGTAGTTTTTTTTTTTTTTTGATGAAATATCTCATGGTAGTTTATAAACCAGTTTCATCAAGAATAATATTTAGTTCTACTTTCCTGCTTAAATAATAGTCAAAATACTACAGAATGCTGTTTGGGAACACTAGAATTGTTCCTTTCTGTTTATTACTTAAGTCAACATAAAGTTATCTTGGTAACTCCATTTTTTGAGTAAACTATCCTCTTTTCATGGTAGTTTAATACTATCGTTGATAAGCCTAAACTTTGGGGGGTCTGGAAGATGTAAAATGTTGAATTTTAACGGGTGCTCATAAATGATTTTTCTGGCAAGCTCAGAACTTTGAAGTATTTCTCGGCCTAGATTGAGTTGAGAAATAGATGCTAAAAGAAAGTGTTTTTTCACTCCCATCTCGAGATTTCCAATCCTTGATCAGTTGTGGACTGGTTCCATAAATCTCATTATTCGCAAAGAAATAGACGAAACATTATCACTTAGTTTCAAACTTGTTATATTTGCTTTTGAAAATATTAACTCGAGGGCTTAACCAGGTCGTCATGAACTGCTGGAAAGTATAGGCCTCAGTCCTGATGCAGTTGCTCGTGATGGGGATGCATTAGCTTTATCAGAATTGTCTTTGAAGTTCCTTGCACCACTTAAAGTATGATTTACTTTGTTGTTACATGTGATGAGTCTGATGATTGATGACCAATAAACCATTTTCGCTATAAAATTTGCACCGAATAATTTACTTAATATCTTACTACTCTTGCTGTCGTGATGCACCCAACCAGAGTGGAGACAGATTTGTTGTTAAGGTGAGGATCTCAGGCACTTCAGCTGCTCGCTTCTACATTGATCATTTCATCTTCAAGCTTCCAAATATGGAGGTAACTATAATGGAACCATTAATCTGCATCTCAGTTTATTAACCTTGCCTGTCTGCCCTGTTAACTTTACTATCCCACATTGTCTTATTTTCTTCCCCTAGCCAATCTTGGAGGCAAAGGGTACAGCAGTTTGGCTTAATAAAAGTTACCGTCCGGTGCGTATCCCGCAAGAATTTCTATCCAAATTTGCTCAATTTGTTCGAGATCGTGAATCAAAGTGA

mRNA sequence

ATGTTGCAGGCTTCCATTAATCCGATCCAATCGCCGCCTCCGGTGTTCAATAACAACACTACGGGACCCCCAATTCCTTCATCAATTGCCTGGCCCATCAGCCGGCCGTCGACATCGTTACGCCTACGTTCGCCCCCAGTAACGAGAAGCTCCAACTCTCAAACATTTGATCTCAAATCGGGCAAAGGGATGGATGGCTTCCTAGATGTTGAGCTCAAAGTTCGTGATTATGAGCTGGATCAGTATGGTGTTGTGAACAATGCTGTCTATGCAAGTTATTGCCAACATGGTCGTCATGAACTGCTGGAAAGTATAGGCCTCAGTCCTGATGCAGTTGCTCGTGATGGGGATGCATTAGCTTTATCAGAATTGTCTTTGAAGTTCCTTGCACCACTTAAAAGTGGAGACAGATTTGTTGTTAAGGTGAGGATCTCAGGCACTTCAGCTGCTCGCTTCTACATTGATCATTTCATCTTCAAGCTTCCAAATATGGAGCCAATCTTGGAGGCAAAGGGTACAGCAGTTTGGCTTAATAAAAGTTACCGTCCGGTGCGTATCCCGCAAGAATTTCTATCCAAATTTGCTCAATTTGTTCGAGATCGTGAATCAAAGTGA

Coding sequence (CDS)

ATGTTGCAGGCTTCCATTAATCCGATCCAATCGCCGCCTCCGGTGTTCAATAACAACACTACGGGACCCCCAATTCCTTCATCAATTGCCTGGCCCATCAGCCGGCCGTCGACATCGTTACGCCTACGTTCGCCCCCAGTAACGAGAAGCTCCAACTCTCAAACATTTGATCTCAAATCGGGCAAAGGGATGGATGGCTTCCTAGATGTTGAGCTCAAAGTTCGTGATTATGAGCTGGATCAGTATGGTGTTGTGAACAATGCTGTCTATGCAAGTTATTGCCAACATGGTCGTCATGAACTGCTGGAAAGTATAGGCCTCAGTCCTGATGCAGTTGCTCGTGATGGGGATGCATTAGCTTTATCAGAATTGTCTTTGAAGTTCCTTGCACCACTTAAAAGTGGAGACAGATTTGTTGTTAAGGTGAGGATCTCAGGCACTTCAGCTGCTCGCTTCTACATTGATCATTTCATCTTCAAGCTTCCAAATATGGAGCCAATCTTGGAGGCAAAGGGTACAGCAGTTTGGCTTAATAAAAGTTACCGTCCGGTGCGTATCCCGCAAGAATTTCTATCCAAATTTGCTCAATTTGTTCGAGATCGTGAATCAAAGTGA

Protein sequence

MLQASINPIQSPPPVFNNNTTGPPIPSSIAWPISRPSTSLRLRSPPVTRSSNSQTFDLKSGKGMDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALALSELSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNKSYRPVRIPQEFLSKFAQFVRDRESK

Homology

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BLAST of Lag0040309 vs. NCBI nr
Match: XP_038892747.1 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 361.7 bits (927), Expect = 4.2e-96
Identity = 180/204 (88.24%), Postives = 191/204 (93.63%), Query Frame = 0


Query: 1   MLQASINPIQSPPPVFNNNTTGPPIPSSIAWPISRPSTSLRLRSPPVTRSSNSQTFDLKS 60
           M QASI PI SPPP+F+NNT+G PI  S+ +PISRPSTSL LRSPPVTRSS + +FDLK 
Sbjct: 1   MFQASIFPIPSPPPIFHNNTSGLPISPSVFFPISRPSTSLHLRSPPVTRSSITSSFDLKP 60

Query: 61  GKGMDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALA 120
           GKGMDGFLD+ELKVRDYELDQ+GVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALA
Sbjct: 61  GKGMDGFLDIELKVRDYELDQFGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALA 120

Query: 121 LSELSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNKS 180
           LSELSLKFLAPL++GDRFVVKVRISGTSAARFY DHFIFKLPNMEPILEAKGTAVWLNKS
Sbjct: 121 LSELSLKFLAPLRTGDRFVVKVRISGTSAARFYFDHFIFKLPNMEPILEAKGTAVWLNKS 180

Query: 181 YRPVRIPQEFLSKFAQFVRDRESK 205
           YRPVRIPQEFLSKFAQFVRD ESK
Sbjct: 181 YRPVRIPQEFLSKFAQFVRDHESK 204

[Close]

BLAST of Lag0040309 vs. NCBI nr
Match: XP_022968132.1 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like [Cucurbita maxima] >XP_022968134.1 acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 351.7 bits (901), Expect = 4.3e-93
Identity = 181/205 (88.29%), Postives = 187/205 (91.22%), Query Frame = 0


Query: 1   MLQASINPIQSPPPVFNNNTTGPPIPSSIAWPISRPS-TSLRLRSPPVTRSSNSQTFDLK 60
           MLQASINPIQSPPP F+  T G   PSS+  PISRPS TSLRLRSP VTRSS S +F LK
Sbjct: 1   MLQASINPIQSPPPAFHYKTAGLAFPSSLVCPISRPSPTSLRLRSPSVTRSSISSSFQLK 60

Query: 61  SGKGMDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL 120
           +GKGMDGFLDVELKVRDYELDQ+GVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL
Sbjct: 61  AGKGMDGFLDVELKVRDYELDQFGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL 120

Query: 121 ALSELSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNK 180
           ALSELSLKFLAPLKSGDRFVVKVRISG SAARFYIDHFIFKLPNMEPILEAK TAVWLNK
Sbjct: 121 ALSELSLKFLAPLKSGDRFVVKVRISGMSAARFYIDHFIFKLPNMEPILEAKATAVWLNK 180

Query: 181 SYRPVRIPQEFLSKFAQFVRDRESK 205
           SYRPVRIPQEF SKFAQFVRD +SK
Sbjct: 181 SYRPVRIPQEFQSKFAQFVRDHDSK 205

[Close]

BLAST of Lag0040309 vs. NCBI nr
Match: XP_023541637.1 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 350.5 bits (898), Expect = 9.7e-93
Identity = 180/205 (87.80%), Postives = 188/205 (91.71%), Query Frame = 0


Query: 1   MLQASINPIQSPPPVFNNNTTGPPIPSSIAWPISRPS-TSLRLRSPPVTRSSNSQTFDLK 60
           MLQASINPIQSPPP F+  T G   PSS+A  ISRPS TSLRLRSP VTR+S S +F LK
Sbjct: 1   MLQASINPIQSPPPAFHYKTAGLAFPSSLACTISRPSPTSLRLRSPSVTRTSISSSFQLK 60

Query: 61  SGKGMDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL 120
           +GKGMDGFLDVELKVRDYELDQ+GVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL
Sbjct: 61  AGKGMDGFLDVELKVRDYELDQFGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL 120

Query: 121 ALSELSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNK 180
           ALSEL+LKFLAPLKSGDRFVVKVRISG SAARFYIDHFIFKLPNMEPILEAKGTAVWLNK
Sbjct: 121 ALSELTLKFLAPLKSGDRFVVKVRISGMSAARFYIDHFIFKLPNMEPILEAKGTAVWLNK 180

Query: 181 SYRPVRIPQEFLSKFAQFVRDRESK 205
           SYRPVRIP EFLSKFAQFVRD +SK
Sbjct: 181 SYRPVRIPHEFLSKFAQFVRDHDSK 205

[Close]

BLAST of Lag0040309 vs. NCBI nr
Match: XP_004135087.1 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic [Cucumis sativus])

HSP 1 Score: 349.7 bits (896), Expect = 1.6e-92
Identity = 173/204 (84.80%), Postives = 186/204 (91.18%), Query Frame = 0


Query: 1   MLQASINPIQSPPPVFNNNTTGPPIPSSIAWPISRPSTSLRLRSPPVTRSSNSQTFDLKS 60
           MLQ+SI PIQSP P+FN  T G P+P S+ +PI+RPSTSLRLRS P TRSS S +FDLK+
Sbjct: 1   MLQSSITPIQSPSPIFNQKTNGFPVPPSVVFPIARPSTSLRLRSLPATRSSISSSFDLKA 60

Query: 61  GKGMDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALA 120
           GKGMD FLD+ELKVRDYELDQ+GVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALA
Sbjct: 61  GKGMDSFLDIELKVRDYELDQFGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALA 120

Query: 121 LSELSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNKS 180
           LSELSLKFLAPL+SGD+FVVKVRISG S ARFYIDH IFKLPNMEPILEAKGTAVWLNKS
Sbjct: 121 LSELSLKFLAPLRSGDKFVVKVRISGISVARFYIDHLIFKLPNMEPILEAKGTAVWLNKS 180

Query: 181 YRPVRIPQEFLSKFAQFVRDRESK 205
           YRPVRIPQEFLSKF QFV+D ESK
Sbjct: 181 YRPVRIPQEFLSKFGQFVQDHESK 204

[Close]

BLAST of Lag0040309 vs. NCBI nr
Match: KAE8648792.1 (hypothetical protein Csa_009266 [Cucumis sativus])

HSP 1 Score: 348.2 bits (892), Expect = 4.8e-92
Identity = 172/203 (84.73%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 1   MLQASINPIQSPPPVFNNNTTGPPIPSSIAWPISRPSTSLRLRSPPVTRSSNSQTFDLKS 60
           MLQ+SI PIQSP P+FN  T G P+P S+ +PI+RPSTSLRLRS P TRSS S +FDLK+
Sbjct: 352 MLQSSITPIQSPSPIFNQKTNGFPVPPSVVFPIARPSTSLRLRSLPATRSSISSSFDLKA 411

Query: 61  GKGMDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALA 120
           GKGMD FLD+ELKVRDYELDQ+GVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALA
Sbjct: 412 GKGMDSFLDIELKVRDYELDQFGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALA 471

Query: 121 LSELSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNKS 180
           LSELSLKFLAPL+SGD+FVVKVRISG S ARFYIDH IFKLPNMEPILEAKGTAVWLNKS
Sbjct: 472 LSELSLKFLAPLRSGDKFVVKVRISGISVARFYIDHLIFKLPNMEPILEAKGTAVWLNKS 531

Query: 181 YRPVRIPQEFLSKFAQFVRDRES 204
           YRPVRIPQEFLSKF QFV+D ES
Sbjct: 532 YRPVRIPQEFLSKFGQFVQDHES 554

[Close]

BLAST of Lag0040309 vs. ExPASy Swiss-Prot
Match: S4TE15 (Acyl-acyl carrier protein thioesterase TE3, chloroplastic OS=Humulus lupulus OX=3486 GN=TE3 PE=2 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 5.5e-67
Identity = 132/200 (66.00%), Postives = 156/200 (78.00%), Query Frame = 0


Query: 5   SINPIQSPPPVFNNNTTGPPIPSSIAWPISRPSTSLRLR-SPPVTRSSNSQTFDLKSGKG 64
           S  P+  P    + +++     SS+A+P++R     RLR  P   R  ++  FD++ GKG
Sbjct: 8   SYKPLHLPISSLSLSSSSSSSASSVAFPVTRLLIPPRLRVLPNPRRRCSALPFDIRGGKG 67

Query: 65  MDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALALSE 124
           M  F +VELKVRDYELDQYGVVNNAVYASYCQHGRHELLES GLS DAVAR+GDALALSE
Sbjct: 68  MSEFYEVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESFGLSCDAVARNGDALALSE 127

Query: 125 LSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNKSYRP 184
           LSLKFLAPL+SGD+FVVKVR+SG+SAAR Y DH IFKLPN EPIL+AKGTAVWL+K+YRP
Sbjct: 128 LSLKFLAPLRSGDKFVVKVRLSGSSAARLYFDHLIFKLPNQEPILDAKGTAVWLDKNYRP 187

Query: 185 VRIPQEFLSKFAQFVRDRES 204
           VRIP E  SK  QF+R+ ES
Sbjct: 188 VRIPPEVRSKLVQFLRNEES 207

[Close]

BLAST of Lag0040309 vs. ExPASy Swiss-Prot
Match: Q8W583 (Acyl-acyl carrier protein thioesterase ATL3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALT3 PE=2 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 1.5e-59
Identity = 118/184 (64.13%), Postives = 138/184 (75.00%), Query Frame = 0


Query: 20  TTGPPIPSSIAWPISRPSTSLRLRSPPVTRSSNSQTFDLKSGKGMDGFLDVELKVRDYEL 79
           T  P +P+ +     R   S+ LRS  V        FDLK GKGM  F +VELKVRDYEL
Sbjct: 8   TATPAMPAVVFLNSWRRPLSIPLRS--VKTFKPLAFFDLKGGKGMSEFHEVELKVRDYEL 67

Query: 80  DQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALALSELSLKFLAPLKSGDRFV 139
           DQ+GVVNNAVYA+YCQHGRHE LESIG++ D VAR G+ALA+SEL++KFL+PL+SGD+FV
Sbjct: 68  DQFGVVNNAVYANYCQHGRHEFLESIGINCDEVARSGEALAISELTMKFLSPLRSGDKFV 127

Query: 140 VKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNKSYRPVRIPQEFLSKFAQFVR 199
           VK RISGTSAAR Y DHFIFKLPN EPILEAKG AVWL+  YRPVRIP    SKF  F+R
Sbjct: 128 VKARISGTSAARIYFDHFIFKLPNQEPILEAKGIAVWLDNKYRPVRIPSSIRSKFVHFLR 187

Query: 200 DRES 204
             ++
Sbjct: 188 QDDA 189

[Close]

BLAST of Lag0040309 vs. ExPASy Swiss-Prot
Match: F4HX80 (Acyl-acyl carrier protein thioesterase ATL4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALT4 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 3.5e-53
Identity = 113/186 (60.75%), Postives = 134/186 (72.04%), Query Frame = 0


Query: 21  TGPPIPS-SIAWPIS-RPSTSLRLRSPPVTRSSNSQTF-DLKSGKGMDGFLDVELKVRDY 80
           TG   P+ S+ +P S R    L LRS    ++    TF DLK GK M  F +VELKVRDY
Sbjct: 5   TGTAAPAMSVVFPTSWRQPVMLPLRS---AKTFKPHTFLDLKGGKEMSEFHEVELKVRDY 64

Query: 81  ELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALALSELSLKFLAPLKSGDR 140
           ELDQ+GVVNNAVYA+YCQHG HE LESIG++ D VAR G+ALA+SEL++ FLAPL+SGD+
Sbjct: 65  ELDQFGVVNNAVYANYCQHGMHEFLESIGINCDEVARSGEALAISELTMNFLAPLRSGDK 124

Query: 141 FVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNKSYRPVRIPQEFLSKFAQF 200
           FVVKV IS TSAAR Y DH I KLPN E ILEAK T VWL+  +RPVRIP    SKF  F
Sbjct: 125 FVVKVNISRTSAARIYFDHSILKLPNQEVILEAKATVVWLDNKHRPVRIPSSIRSKFVHF 184

Query: 201 VRDRES 204
           +R  ++
Sbjct: 185 LRQNDT 187

[Close]

BLAST of Lag0040309 vs. ExPASy Swiss-Prot
Match: Q9C7I5 (Acyl-acyl carrier protein thioesterase ATL1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALT1 PE=1 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.1e-51
Identity = 96/144 (66.67%), Postives = 116/144 (80.56%), Query Frame = 0


Query: 56  FDLKSGKGMDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARD 115
           F  + GKGM+G  ++ELKVRDYELDQ+GVVNNAVYA+YCQHG+HE +E+IG++ D V+R 
Sbjct: 40  FKQQGGKGMNGVHEIELKVRDYELDQFGVVNNAVYANYCQHGQHEFMETIGINCDEVSRS 99

Query: 116 GDALALSELSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAV 175
           G+ALA+SEL++KFLAPL+SG +FVVK RISGTS  R Y + FIFKLPN EPILEAKG AV
Sbjct: 100 GEALAVSELTIKFLAPLRSGCKFVVKTRISGTSMTRIYFEQFIFKLPNQEPILEAKGMAV 159

Query: 176 WLNKSYRPVRIPQEFLSKFAQFVR 200
           WL+K YRPV IP    S F  F R
Sbjct: 160 WLDKRYRPVCIPSYIRSNFGHFQR 183

[Close]

BLAST of Lag0040309 vs. ExPASy Swiss-Prot
Match: Q9C7I8 (Acyl-acyl carrier protein thioesterase ATL2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALT2 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 5.2e-49
Identity = 103/184 (55.98%), Postives = 129/184 (70.11%), Query Frame = 0


Query: 15  VFNNNTTGPPIPSSIAWPIS-RPSTSLRLRSPPVTRSSNSQTFDLKSGKGMDGFLDVELK 74
           +F   +TG  I  + A+P S R    L LRS  + +       +L+   G+ GF ++ELK
Sbjct: 1   MFQATSTGAQIMHA-AFPRSWRRGHVLPLRSAKIFKP--LACLELRGSTGIGGFHEIELK 60

Query: 75  VRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALALSELSLKFLAPLK 134
           VRDYELDQ+GVVNNAVYA+YCQHGRHE ++SIG++ + V+R G ALA+ EL++KFLAPL+
Sbjct: 61  VRDYELDQFGVVNNAVYANYCQHGRHEFMDSIGINCNEVSRSGGALAIPELTIKFLAPLR 120

Query: 135 SGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNKSYRPVRIPQEFLSK 194
           SG RFVVK RISG S  R Y + FIFKLPN EPILEAKGTAVWL+  YRP R+P    S 
Sbjct: 121 SGCRFVVKTRISGISLVRIYFEQFIFKLPNQEPILEAKGTAVWLDNKYRPTRVPSHVRSY 180

Query: 195 FAQF 198
           F  F
Sbjct: 181 FGHF 181

[Close]

BLAST of Lag0040309 vs. ExPASy TrEMBL
Match: A0A6J1HST6 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111467460 PE=4 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 2.1e-93
Identity = 181/205 (88.29%), Postives = 187/205 (91.22%), Query Frame = 0


Query: 1   MLQASINPIQSPPPVFNNNTTGPPIPSSIAWPISRPS-TSLRLRSPPVTRSSNSQTFDLK 60
           MLQASINPIQSPPP F+  T G   PSS+  PISRPS TSLRLRSP VTRSS S +F LK
Sbjct: 1   MLQASINPIQSPPPAFHYKTAGLAFPSSLVCPISRPSPTSLRLRSPSVTRSSISSSFQLK 60

Query: 61  SGKGMDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL 120
           +GKGMDGFLDVELKVRDYELDQ+GVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL
Sbjct: 61  AGKGMDGFLDVELKVRDYELDQFGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL 120

Query: 121 ALSELSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNK 180
           ALSELSLKFLAPLKSGDRFVVKVRISG SAARFYIDHFIFKLPNMEPILEAK TAVWLNK
Sbjct: 121 ALSELSLKFLAPLKSGDRFVVKVRISGMSAARFYIDHFIFKLPNMEPILEAKATAVWLNK 180

Query: 181 SYRPVRIPQEFLSKFAQFVRDRESK 205
           SYRPVRIPQEF SKFAQFVRD +SK
Sbjct: 181 SYRPVRIPQEFQSKFAQFVRDHDSK 205

[Close]

BLAST of Lag0040309 vs. ExPASy TrEMBL
Match: A0A6J1DA66 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111018616 PE=4 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 9.8e-91
Identity = 172/204 (84.31%), Postives = 183/204 (89.71%), Query Frame = 0


Query: 1   MLQASINPIQSPPPVFNNNTTGPPIPSSIAWPISRPSTSLRLRSPPVTRSSNSQTFDLKS 60
           MLQASI PIQ P P+    +T  PIPSS +WPI RPSTS RLRSPP+TRSSNSQ FDL +
Sbjct: 1   MLQASIYPIQPPAPISRTKSTILPIPSSFSWPIRRPSTSFRLRSPPITRSSNSQAFDLNA 60

Query: 61  GKGMDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALA 120
            K MDGFLDVELKVRDYELDQ+GVVNNAVYASYCQHGRHELLESIGLSPDAVAR GDALA
Sbjct: 61  TKRMDGFLDVELKVRDYELDQFGVVNNAVYASYCQHGRHELLESIGLSPDAVARTGDALA 120

Query: 121 LSELSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNKS 180
           LSELSLKFLAPL+SGDRFVVKVRIS  SAARFYIDHFIFKLP+MEPILEAKGTAVWLNK+
Sbjct: 121 LSELSLKFLAPLRSGDRFVVKVRISSMSAARFYIDHFIFKLPDMEPILEAKGTAVWLNKN 180

Query: 181 YRPVRIPQEFLSKFAQFVRDRESK 205
           YRPVRIPQEFLSKFA+F R+ ESK
Sbjct: 181 YRPVRIPQEFLSKFAEFARNHESK 204

[Close]

BLAST of Lag0040309 vs. ExPASy TrEMBL
Match: A0A6J1G0B9 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111449530 PE=4 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 2.2e-90
Identity = 175/205 (85.37%), Postives = 186/205 (90.73%), Query Frame = 0


Query: 1   MLQASINPIQSPPPVFNNNTTGPPIPSSIAWPISRPS-TSLRLRSPPVTRSSNSQTFDLK 60
           MLQASINPI+SPPP F+  T G  +PSS++  ISRPS TSLRLRS  VTR+S S +F LK
Sbjct: 1   MLQASINPIRSPPPAFHYKTAGLALPSSLSCTISRPSPTSLRLRSSSVTRTSISSSFQLK 60

Query: 61  SGKGMDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL 120
           +GKGMDGFLDVELKVRDYELDQ+GVVNNAVYASYCQHGRHELLESIGLSPD VARDGDAL
Sbjct: 61  AGKGMDGFLDVELKVRDYELDQFGVVNNAVYASYCQHGRHELLESIGLSPDEVARDGDAL 120

Query: 121 ALSELSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNK 180
           ALSELSLKFLAPLKSGDRFV+KVRISG SAA FYIDHFIFKLPNMEPILEAKGTAVWLNK
Sbjct: 121 ALSELSLKFLAPLKSGDRFVLKVRISGMSAAHFYIDHFIFKLPNMEPILEAKGTAVWLNK 180

Query: 181 SYRPVRIPQEFLSKFAQFVRDRESK 205
           SYRPVRIP EFLSKFAQFVRD +SK
Sbjct: 181 SYRPVRIPHEFLSKFAQFVRDHDSK 205

[Close]

BLAST of Lag0040309 vs. ExPASy TrEMBL
Match: A0A5A7SVA0 (Acyl-acyl carrier protein thioesterase ATL3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G005840 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 6.3e-90
Identity = 171/205 (83.41%), Postives = 186/205 (90.73%), Query Frame = 0


Query: 1   MLQASINPIQSPPPVFNNNTTGPPIPSSIAWPISRPSTSLRLRSPPVTRSS-NSQTFDLK 60
           MLQASI PIQSPPP+F+  T G P P S+ +P +R STSLRL+S PVTRSS +S +FDLK
Sbjct: 351 MLQASITPIQSPPPIFHEKTNGFPTPPSVVFPSARRSTSLRLQSLPVTRSSISSSSFDLK 410

Query: 61  SGKGMDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL 120
           +GKGMD FLD+ELKVRDYELDQ+GVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL
Sbjct: 411 AGKGMDTFLDIELKVRDYELDQFGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL 470

Query: 121 ALSELSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNK 180
           ALSELSLKFLAPL+SGD+FVVKVRISG S ARFYIDHFIFKLPNMEPILEAKGTAVWLNK
Sbjct: 471 ALSELSLKFLAPLRSGDKFVVKVRISGISVARFYIDHFIFKLPNMEPILEAKGTAVWLNK 530

Query: 181 SYRPVRIPQEFLSKFAQFVRDRESK 205
           +YRPVRIPQEFLSKF QFV+D E K
Sbjct: 531 NYRPVRIPQEFLSKFGQFVQDHECK 555

[Close]

BLAST of Lag0040309 vs. ExPASy TrEMBL
Match: A0A1S3BG49 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489290 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 6.3e-90
Identity = 171/205 (83.41%), Postives = 186/205 (90.73%), Query Frame = 0


Query: 1   MLQASINPIQSPPPVFNNNTTGPPIPSSIAWPISRPSTSLRLRSPPVTRSS-NSQTFDLK 60
           MLQASI PIQSPPP+F+  T G P P S+ +P +R STSLRL+S PVTRSS +S +FDLK
Sbjct: 1   MLQASITPIQSPPPIFHEKTNGFPTPPSVVFPSARRSTSLRLQSLPVTRSSISSSSFDLK 60

Query: 61  SGKGMDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL 120
           +GKGMD FLD+ELKVRDYELDQ+GVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL
Sbjct: 61  AGKGMDTFLDIELKVRDYELDQFGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDAL 120

Query: 121 ALSELSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNK 180
           ALSELSLKFLAPL+SGD+FVVKVRISG S ARFYIDHFIFKLPNMEPILEAKGTAVWLNK
Sbjct: 121 ALSELSLKFLAPLRSGDKFVVKVRISGISVARFYIDHFIFKLPNMEPILEAKGTAVWLNK 180

Query: 181 SYRPVRIPQEFLSKFAQFVRDRESK 205
           +YRPVRIPQEFLSKF QFV+D E K
Sbjct: 181 NYRPVRIPQEFLSKFGQFVQDHECK 205

[Close]

BLAST of Lag0040309 vs. TAIR 10
Match: AT1G68260.1 (Thioesterase superfamily protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.0e-60
Identity = 118/184 (64.13%), Postives = 138/184 (75.00%), Query Frame = 0


Query: 20  TTGPPIPSSIAWPISRPSTSLRLRSPPVTRSSNSQTFDLKSGKGMDGFLDVELKVRDYEL 79
           T  P +P+ +     R   S+ LRS  V        FDLK GKGM  F +VELKVRDYEL
Sbjct: 8   TATPAMPAVVFLNSWRRPLSIPLRS--VKTFKPLAFFDLKGGKGMSEFHEVELKVRDYEL 67

Query: 80  DQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALALSELSLKFLAPLKSGDRFV 139
           DQ+GVVNNAVYA+YCQHGRHE LESIG++ D VAR G+ALA+SEL++KFL+PL+SGD+FV
Sbjct: 68  DQFGVVNNAVYANYCQHGRHEFLESIGINCDEVARSGEALAISELTMKFLSPLRSGDKFV 127

Query: 140 VKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNKSYRPVRIPQEFLSKFAQFVR 199
           VK RISGTSAAR Y DHFIFKLPN EPILEAKG AVWL+  YRPVRIP    SKF  F+R
Sbjct: 128 VKARISGTSAARIYFDHFIFKLPNQEPILEAKGIAVWLDNKYRPVRIPSSIRSKFVHFLR 187

Query: 200 DRES 204
             ++
Sbjct: 188 QDDA 189

[Close]

BLAST of Lag0040309 vs. TAIR 10
Match: AT1G68280.1 (Thioesterase superfamily protein )

HSP 1 Score: 209.5 bits (532), Expect = 2.5e-54
Identity = 113/186 (60.75%), Postives = 134/186 (72.04%), Query Frame = 0


Query: 21  TGPPIPS-SIAWPIS-RPSTSLRLRSPPVTRSSNSQTF-DLKSGKGMDGFLDVELKVRDY 80
           TG   P+ S+ +P S R    L LRS    ++    TF DLK GK M  F +VELKVRDY
Sbjct: 5   TGTAAPAMSVVFPTSWRQPVMLPLRS---AKTFKPHTFLDLKGGKEMSEFHEVELKVRDY 64

Query: 81  ELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALALSELSLKFLAPLKSGDR 140
           ELDQ+GVVNNAVYA+YCQHG HE LESIG++ D VAR G+ALA+SEL++ FLAPL+SGD+
Sbjct: 65  ELDQFGVVNNAVYANYCQHGMHEFLESIGINCDEVARSGEALAISELTMNFLAPLRSGDK 124

Query: 141 FVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNKSYRPVRIPQEFLSKFAQF 200
           FVVKV IS TSAAR Y DH I KLPN E ILEAK T VWL+  +RPVRIP    SKF  F
Sbjct: 125 FVVKVNISRTSAARIYFDHSILKLPNQEVILEAKATVVWLDNKHRPVRIPSSIRSKFVHF 184

Query: 201 VRDRES 204
           +R  ++
Sbjct: 185 LRQNDT 187

[Close]

BLAST of Lag0040309 vs. TAIR 10
Match: AT1G35290.1 (Thioesterase superfamily protein )

HSP 1 Score: 204.5 bits (519), Expect = 8.0e-53
Identity = 96/144 (66.67%), Postives = 116/144 (80.56%), Query Frame = 0


Query: 56  FDLKSGKGMDGFLDVELKVRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARD 115
           F  + GKGM+G  ++ELKVRDYELDQ+GVVNNAVYA+YCQHG+HE +E+IG++ D V+R 
Sbjct: 40  FKQQGGKGMNGVHEIELKVRDYELDQFGVVNNAVYANYCQHGQHEFMETIGINCDEVSRS 99

Query: 116 GDALALSELSLKFLAPLKSGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAV 175
           G+ALA+SEL++KFLAPL+SG +FVVK RISGTS  R Y + FIFKLPN EPILEAKG AV
Sbjct: 100 GEALAVSELTIKFLAPLRSGCKFVVKTRISGTSMTRIYFEQFIFKLPNQEPILEAKGMAV 159

Query: 176 WLNKSYRPVRIPQEFLSKFAQFVR 200
           WL+K YRPV IP    S F  F R
Sbjct: 160 WLDKRYRPVCIPSYIRSNFGHFQR 183

[Close]

BLAST of Lag0040309 vs. TAIR 10
Match: AT1G35250.1 (Thioesterase superfamily protein )

HSP 1 Score: 195.7 bits (496), Expect = 3.7e-50
Identity = 103/184 (55.98%), Postives = 129/184 (70.11%), Query Frame = 0


Query: 15  VFNNNTTGPPIPSSIAWPIS-RPSTSLRLRSPPVTRSSNSQTFDLKSGKGMDGFLDVELK 74
           +F   +TG  I  + A+P S R    L LRS  + +       +L+   G+ GF ++ELK
Sbjct: 1   MFQATSTGAQIMHA-AFPRSWRRGHVLPLRSAKIFKP--LACLELRGSTGIGGFHEIELK 60

Query: 75  VRDYELDQYGVVNNAVYASYCQHGRHELLESIGLSPDAVARDGDALALSELSLKFLAPLK 134
           VRDYELDQ+GVVNNAVYA+YCQHGRHE ++SIG++ + V+R G ALA+ EL++KFLAPL+
Sbjct: 61  VRDYELDQFGVVNNAVYANYCQHGRHEFMDSIGINCNEVSRSGGALAIPELTIKFLAPLR 120

Query: 135 SGDRFVVKVRISGTSAARFYIDHFIFKLPNMEPILEAKGTAVWLNKSYRPVRIPQEFLSK 194
           SG RFVVK RISG S  R Y + FIFKLPN EPILEAKGTAVWL+  YRP R+P    S 
Sbjct: 121 SGCRFVVKTRISGISLVRIYFEQFIFKLPNQEPILEAKGTAVWLDNKYRPTRVPSHVRSY 180

Query: 195 FAQF 198
           F  F
Sbjct: 181 FGHF 181

The following BLAST results are available for this feature:

BLAST of Lag0040309 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892747.1	4.2e-96	88.24	acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like [Benincasa hispi...	[more]
XP_022968132.1	4.3e-93	88.29	acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like [Cucurbita maxim...	[more]
XP_023541637.1	9.7e-93	87.80	acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like isoform X2 [Cucu...	[more]
XP_004135087.1	1.6e-92	84.80	acyl-acyl carrier protein thioesterase ATL3, chloroplastic [Cucumis sativus]	[more]
KAE8648792.1	4.8e-92	84.73	hypothetical protein Csa_009266 [Cucumis sativus]	[more]

BLAST of Lag0040309 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
S4TE15	5.5e-67	66.00	Acyl-acyl carrier protein thioesterase TE3, chloroplastic OS=Humulus lupulus OX=...	[more]
Q8W583	1.5e-59	64.13	Acyl-acyl carrier protein thioesterase ATL3, chloroplastic OS=Arabidopsis thalia...	[more]
F4HX80	3.5e-53	60.75	Acyl-acyl carrier protein thioesterase ATL4, chloroplastic OS=Arabidopsis thalia...	[more]
Q9C7I5	1.1e-51	66.67	Acyl-acyl carrier protein thioesterase ATL1, chloroplastic OS=Arabidopsis thalia...	[more]
Q9C7I8	5.2e-49	55.98	Acyl-acyl carrier protein thioesterase ATL2, chloroplastic OS=Arabidopsis thalia...	[more]

BLAST of Lag0040309 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HST6	2.1e-93	88.29	acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like OS=Cucurbita max...	[more]
A0A6J1DA66	9.8e-91	84.31	acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like OS=Momordica cha...	[more]
A0A6J1G0B9	2.2e-90	85.37	acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like OS=Cucurbita mos...	[more]
A0A5A7SVA0	6.3e-90	83.41	Acyl-acyl carrier protein thioesterase ATL3 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BG49	6.3e-90	83.41	acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like OS=Cucumis melo ...	[more]

BLAST of Lag0040309 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G68260.1	1.0e-60	64.13	Thioesterase superfamily protein	[more]
AT1G68280.1	2.5e-54	60.75	Thioesterase superfamily protein	[more]
AT1G35290.1	8.0e-53	66.67	Thioesterase superfamily protein	[more]
AT1G35250.1	3.7e-50	55.98	Thioesterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.129.10	Hotdog Thioesterase	coord: 54..200 e-value: 3.8E-33 score: 116.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..30
None	No IPR available	PANTHER	PTHR31793:SF27	ACYL-ACYL CARRIER PROTEIN THIOESTERASE ATL1, CHLOROPLASTIC-RELATED	coord: 34..203
None	No IPR available	PANTHER	PTHR31793	4-HYDROXYBENZOYL-COA THIOESTERASE FAMILY MEMBER	coord: 34..203
None	No IPR available	CDD	cd00586	4HBT	coord: 70..178 e-value: 8.90112E-30 score: 104.61
IPR006683	Thioesterase domain	PFAM	PF03061	4HBT	coord: 82..165 e-value: 3.6E-13 score: 49.7
IPR029069	HotDog domain superfamily	SUPERFAMILY	54637	Thioesterase/thiol ester dehydrase-isomerase	coord: 64..199

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0040309.1	Lag0040309.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0016297	acyl-[acyl-carrier-protein] hydrolase activity