Lag0040308 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0040308 vs. NCBI nr
Match: XP_022968132.1 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like [Cucurbita maxima] >XP_022968134.1 acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 227.3 bits (578), Expect = 1.1e-55
Identity = 128/208 (61.54%), Postives = 154/208 (74.04%), Query Frame = 0

Query: 4   LQASFNPIQFP----HTKAS-----------------SSLRLRSPMLIKTSISQTVLHLN 63
           LQAS NPIQ P    H K +                 +SLRLRSP + ++SIS +     
Sbjct: 2   LQASINPIQSPPPAFHYKTAGLAFPSSLVCPISRPSPTSLRLRSPSVTRSSISSS---FQ 61

Query: 64  LKAAR-MNHFQDFELQVRNYELDQFGVVNNAVYANYCSNGRQELMERITGVSADAVARSG 123
           LKA + M+ F D EL+VR+YELDQFGVVNNAVYA+YC +GR EL+E I G+S DAVAR G
Sbjct: 62  LKAGKGMDGFLDVELKVRDYELDQFGVVNNAVYASYCQHGRHELLESI-GLSPDAVARDG 121

Query: 124 NALALSDLSFKFLAPLRSGDRFVLKVRISSTTTTRIYIDHLIFKLPNMESILEAKATAVW 183
           +ALALS+LS KFLAPL+SGDRFV+KVRIS  +  R YIDH IFKLPNME ILEAKATAVW
Sbjct: 122 DALALSELSLKFLAPLKSGDRFVVKVRISGMSAARFYIDHFIFKLPNMEPILEAKATAVW 181

Query: 184 LDQNYRPMRIPQEYLTKLAQFVRDHESK 190
           L+++YRP+RIPQE+ +K AQFVRDH+SK
Sbjct: 182 LNKSYRPVRIPQEFQSKFAQFVRDHDSK 205

BLAST of Lag0040308 vs. NCBI nr
Match: XP_038892747.1 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 226.9 bits (577), Expect = 1.5e-55
Identity = 118/178 (66.29%), Postives = 146/178 (82.02%), Query Frame = 0

Query: 13  FPHTKASSSLRLRSPMLIKTSISQTVLHLNLKAAR-MNHFQDFELQVRNYELDQFGVVNN 72
           FP ++ S+SL LRSP + ++SI+ +    +LK  + M+ F D EL+VR+YELDQFGVVNN
Sbjct: 31  FPISRPSTSLHLRSPPVTRSSITSS---FDLKPGKGMDGFLDIELKVRDYELDQFGVVNN 90

Query: 73  AVYANYCSNGRQELMERITGVSADAVARSGNALALSDLSFKFLAPLRSGDRFVLKVRISS 132
           AVYA+YC +GR EL+E I G+S DAVAR G+ALALS+LS KFLAPLR+GDRFV+KVRIS 
Sbjct: 91  AVYASYCQHGRHELLESI-GLSPDAVARDGDALALSELSLKFLAPLRTGDRFVVKVRISG 150

Query: 133 TTTTRIYIDHLIFKLPNMESILEAKATAVWLDQNYRPMRIPQEYLTKLAQFVRDHESK 190
           T+  R Y DH IFKLPNME ILEAK TAVWL+++YRP+RIPQE+L+K AQFVRDHESK
Sbjct: 151 TSAARFYFDHFIFKLPNMEPILEAKGTAVWLNKSYRPVRIPQEFLSKFAQFVRDHESK 204

BLAST of Lag0040308 vs. NCBI nr
Match: XP_023541637.1 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 226.5 bits (576), Expect = 1.9e-55
Identity = 127/208 (61.06%), Postives = 153/208 (73.56%), Query Frame = 0

Query: 4   LQASFNPIQFP----HTKAS-----------------SSLRLRSPMLIKTSISQTVLHLN 63
           LQAS NPIQ P    H K +                 +SLRLRSP + +TSIS +     
Sbjct: 2   LQASINPIQSPPPAFHYKTAGLAFPSSLACTISRPSPTSLRLRSPSVTRTSISSS---FQ 61

Query: 64  LKAAR-MNHFQDFELQVRNYELDQFGVVNNAVYANYCSNGRQELMERITGVSADAVARSG 123
           LKA + M+ F D EL+VR+YELDQFGVVNNAVYA+YC +GR EL+E I G+S DAVAR G
Sbjct: 62  LKAGKGMDGFLDVELKVRDYELDQFGVVNNAVYASYCQHGRHELLESI-GLSPDAVARDG 121

Query: 124 NALALSDLSFKFLAPLRSGDRFVLKVRISSTTTTRIYIDHLIFKLPNMESILEAKATAVW 183
           +ALALS+L+ KFLAPL+SGDRFV+KVRIS  +  R YIDH IFKLPNME ILEAK TAVW
Sbjct: 122 DALALSELTLKFLAPLKSGDRFVVKVRISGMSAARFYIDHFIFKLPNMEPILEAKGTAVW 181

Query: 184 LDQNYRPMRIPQEYLTKLAQFVRDHESK 190
           L+++YRP+RIP E+L+K AQFVRDH+SK
Sbjct: 182 LNKSYRPVRIPHEFLSKFAQFVRDHDSK 205

BLAST of Lag0040308 vs. NCBI nr
Match: XP_023541635.1 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023541636.1 acyl-acyl carrier protein thioesterase ATL3, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 226.5 bits (576), Expect = 1.9e-55
Identity = 128/211 (60.66%), Postives = 153/211 (72.51%), Query Frame = 0

Query: 4   LQASFNPIQFP----HTKAS-----------------SSLRLRSPMLIKTSISQTVLHLN 63
           LQAS NPIQ P    H K +                 +SLRLRSP + +TSIS +     
Sbjct: 2   LQASINPIQSPPPAFHYKTAGLAFPSSLACTISRPSPTSLRLRSPSVTRTSISSS---FQ 61

Query: 64  LKAA----RMNHFQDFELQVRNYELDQFGVVNNAVYANYCSNGRQELMERITGVSADAVA 123
           LKA     RM+ F D EL+VR+YELDQFGVVNNAVYA+YC +GR EL+E I G+S DAVA
Sbjct: 62  LKAGKGHYRMDGFLDVELKVRDYELDQFGVVNNAVYASYCQHGRHELLESI-GLSPDAVA 121

Query: 124 RSGNALALSDLSFKFLAPLRSGDRFVLKVRISSTTTTRIYIDHLIFKLPNMESILEAKAT 183
           R G+ALALS+L+ KFLAPL+SGDRFV+KVRIS  +  R YIDH IFKLPNME ILEAK T
Sbjct: 122 RDGDALALSELTLKFLAPLKSGDRFVVKVRISGMSAARFYIDHFIFKLPNMEPILEAKGT 181

Query: 184 AVWLDQNYRPMRIPQEYLTKLAQFVRDHESK 190
           AVWL+++YRP+RIP E+L+K AQFVRDH+SK
Sbjct: 182 AVWLNKSYRPVRIPHEFLSKFAQFVRDHDSK 208

BLAST of Lag0040308 vs. NCBI nr
Match: XP_004135087.1 (acyl-acyl carrier protein thioesterase ATL3, chloroplastic [Cucumis sativus])

HSP 1 Score: 224.9 bits (572), Expect = 5.7e-55
Identity = 119/180 (66.11%), Postives = 146/180 (81.11%), Query Frame = 0

Query: 11  IQFPHTKASSSLRLRSPMLIKTSISQTVLHLNLKAAR-MNHFQDFELQVRNYELDQFGVV 70
           + FP  + S+SLRLRS    ++SIS +    +LKA + M+ F D EL+VR+YELDQFGVV
Sbjct: 29  VVFPIARPSTSLRLRSLPATRSSISSS---FDLKAGKGMDSFLDIELKVRDYELDQFGVV 88

Query: 71  NNAVYANYCSNGRQELMERITGVSADAVARSGNALALSDLSFKFLAPLRSGDRFVLKVRI 130
           NNAVYA+YC +GR EL+E I G+S DAVAR G+ALALS+LS KFLAPLRSGD+FV+KVRI
Sbjct: 89  NNAVYASYCQHGRHELLESI-GLSPDAVARDGDALALSELSLKFLAPLRSGDKFVVKVRI 148

Query: 131 SSTTTTRIYIDHLIFKLPNMESILEAKATAVWLDQNYRPMRIPQEYLTKLAQFVRDHESK 190
           S  +  R YIDHLIFKLPNME ILEAK TAVWL+++YRP+RIPQE+L+K  QFV+DHESK
Sbjct: 149 SGISVARFYIDHLIFKLPNMEPILEAKGTAVWLNKSYRPVRIPQEFLSKFGQFVQDHESK 204

BLAST of Lag0040308 vs. ExPASy Swiss-Prot
Match: S4TE15 (Acyl-acyl carrier protein thioesterase TE3, chloroplastic OS=Humulus lupulus OX=3486 GN=TE3 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 2.2e-49
Identity = 104/184 (56.52%), Postives = 138/184 (75.00%), Query Frame = 0

Query: 6   ASFNPIQFPHTKASSSLRLRSPMLIKTSISQTVLHLNLKAAR-MNHFQDFELQVRNYELD 65
           +S + + FP T+     RLR  +L       + L  +++  + M+ F + EL+VR+YELD
Sbjct: 27  SSASSVAFPVTRLLIPPRLR--VLPNPRRRCSALPFDIRGGKGMSEFYEVELKVRDYELD 86

Query: 66  QFGVVNNAVYANYCSNGRQELMERITGVSADAVARSGNALALSDLSFKFLAPLRSGDRFV 125
           Q+GVVNNAVYA+YC +GR EL+E   G+S DAVAR+G+ALALS+LS KFLAPLRSGD+FV
Sbjct: 87  QYGVVNNAVYASYCQHGRHELLESF-GLSCDAVARNGDALALSELSLKFLAPLRSGDKFV 146

Query: 126 LKVRISSTTTTRIYIDHLIFKLPNMESILEAKATAVWLDQNYRPMRIPQEYLTKLAQFVR 185
           +KVR+S ++  R+Y DHLIFKLPN E IL+AK TAVWLD+NYRP+RIP E  +KL QF+R
Sbjct: 147 VKVRLSGSSAARLYFDHLIFKLPNQEPILDAKGTAVWLDKNYRPVRIPPEVRSKLVQFLR 206

Query: 186 DHES 189
           + ES
Sbjct: 207 NEES 207

BLAST of Lag0040308 vs. ExPASy Swiss-Prot
Match: Q8W583 (Acyl-acyl carrier protein thioesterase ATL3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALT3 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 3.2e-45
Identity = 88/141 (62.41%), Postives = 108/141 (76.60%), Query Frame = 0

Query: 48  MNHFQDFELQVRNYELDQFGVVNNAVYANYCSNGRQELMERITGVSADAVARSGNALALS 107
           M+ F + EL+VR+YELDQFGVVNNAVYANYC +GR E +E I G++ D VARSG ALA+S
Sbjct: 50  MSEFHEVELKVRDYELDQFGVVNNAVYANYCQHGRHEFLESI-GINCDEVARSGEALAIS 109

Query: 108 DLSFKFLAPLRSGDRFVLKVRISSTTTTRIYIDHLIFKLPNMESILEAKATAVWLDQNYR 167
           +L+ KFL+PLRSGD+FV+K RIS T+  RIY DH IFKLPN E ILEAK  AVWLD  YR
Sbjct: 110 ELTMKFLSPLRSGDKFVVKARISGTSAARIYFDHFIFKLPNQEPILEAKGIAVWLDNKYR 169

Query: 168 PMRIPQEYLTKLAQFVRDHES 189
           P+RIP    +K   F+R  ++
Sbjct: 170 PVRIPSSIRSKFVHFLRQDDA 189

BLAST of Lag0040308 vs. ExPASy Swiss-Prot
Match: F4HX80 (Acyl-acyl carrier protein thioesterase ATL4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALT4 PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 8.0e-44
Identity = 92/168 (54.76%), Postives = 120/168 (71.43%), Query Frame = 0

Query: 25  RSPMLIKTSISQTV---LHLNLKAAR-MNHFQDFELQVRNYELDQFGVVNNAVYANYCSN 84
           R P+++    ++T      L+LK  + M+ F + EL+VR+YELDQFGVVNNAVYANYC +
Sbjct: 21  RQPVMLPLRSAKTFKPHTFLDLKGGKEMSEFHEVELKVRDYELDQFGVVNNAVYANYCQH 80

Query: 85  GRQELMERITGVSADAVARSGNALALSDLSFKFLAPLRSGDRFVLKVRISSTTTTRIYID 144
           G  E +E I G++ D VARSG ALA+S+L+  FLAPLRSGD+FV+KV IS T+  RIY D
Sbjct: 81  GMHEFLESI-GINCDEVARSGEALAISELTMNFLAPLRSGDKFVVKVNISRTSAARIYFD 140

Query: 145 HLIFKLPNMESILEAKATAVWLDQNYRPMRIPQEYLTKLAQFVRDHES 189
           H I KLPN E ILEAKAT VWLD  +RP+RIP    +K   F+R +++
Sbjct: 141 HSILKLPNQEVILEAKATVVWLDNKHRPVRIPSSIRSKFVHFLRQNDT 187

BLAST of Lag0040308 vs. ExPASy Swiss-Prot
Match: Q9C7I5 (Acyl-acyl carrier protein thioesterase ATL1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALT1 PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.2e-41
Identity = 85/139 (61.15%), Postives = 103/139 (74.10%), Query Frame = 0

Query: 48  MNHFQDFELQVRNYELDQFGVVNNAVYANYCSNGRQELMERITGVSADAVARSGNALALS 107
           MN   + EL+VR+YELDQFGVVNNAVYANYC +G+ E ME I G++ D V+RSG ALA+S
Sbjct: 48  MNGVHEIELKVRDYELDQFGVVNNAVYANYCQHGQHEFMETI-GINCDEVSRSGEALAVS 107

Query: 108 DLSFKFLAPLRSGDRFVLKVRISSTTTTRIYIDHLIFKLPNMESILEAKATAVWLDQNYR 167
           +L+ KFLAPLRSG +FV+K RIS T+ TRIY +  IFKLPN E ILEAK  AVWLD+ YR
Sbjct: 108 ELTIKFLAPLRSGCKFVVKTRISGTSMTRIYFEQFIFKLPNQEPILEAKGMAVWLDKRYR 167

Query: 168 PMRIPQEYLTKLAQFVRDH 187
           P+ IP    +    F R H
Sbjct: 168 PVCIPSYIRSNFGHFQRQH 185

BLAST of Lag0040308 vs. ExPASy Swiss-Prot
Match: Q9C7I8 (Acyl-acyl carrier protein thioesterase ATL2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALT2 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-39
Identity = 81/136 (59.56%), Postives = 98/136 (72.06%), Query Frame = 0

Query: 51  FQDFELQVRNYELDQFGVVNNAVYANYCSNGRQELMERITGVSADAVARSGNALALSDLS 110
           F + EL+VR+YELDQFGVVNNAVYANYC +GR E M+ I G++ + V+RSG ALA+ +L+
Sbjct: 51  FHEIELKVRDYELDQFGVVNNAVYANYCQHGRHEFMDSI-GINCNEVSRSGGALAIPELT 110

Query: 111 FKFLAPLRSGDRFVLKVRISSTTTTRIYIDHLIFKLPNMESILEAKATAVWLDQNYRPMR 170
            KFLAPLRSG RFV+K RIS  +  RIY +  IFKLPN E ILEAK TAVWLD  YRP R
Sbjct: 111 IKFLAPLRSGCRFVVKTRISGISLVRIYFEQFIFKLPNQEPILEAKGTAVWLDNKYRPTR 170

Query: 171 IPQEYLTKLAQFVRDH 187
           +P    +    F   H
Sbjct: 171 VPSHVRSYFGHFQCQH 185