Lag0040302 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040302
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionVQ motif-containing protein 8, chloroplastic
Locationchr13: 3708290 .. 3708793 (+)
RNA-Seq ExpressionLag0040302
SyntenyLag0040302
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTCCAGCTAAATCCTGCGACCTCAATGGCCCTCGCCCGTCTCCGCTCCTAATTCACAAAGATTCTCGCTTGATTCGCAAGGCTTCTCAGCCGCCGCCGCAGCTCCGGCAGCCGGTCATAATCTACACCCATTCCCCGAAAATCATCCACACGCAGCCCAAGGATTTCATGGCCCTCGTCCAACGCCTCACCGGCTTCACGCCCTCCGACGCCGCCGCCCCAGCTCAACAAACGCTTCAGAAACCGCCCTCCGACTCCGACAGGAATCTCAACGACAATGATTCTTCCTCGGTTCTCACGGACGACGACAATAATAACGACGGCCGTCGTCCTCACAGAGATTGTAATTCGTCGGCTTTGTCCGATGTTCCTCTGTTCACTCCTCGTTCTTCGCTTCTCAGATATTCTTATTCCGATACGGCGCCTTTTTCGTCTCCTGGTTTAGTTAACTCCTTGTCCCCTTCTTTCATGGAGTTTCTCAAAGCCCTCCCTGAATTTTAA

mRNA sequence

ATGAGTCCAGCTAAATCCTGCGACCTCAATGGCCCTCGCCCGTCTCCGCTCCTAATTCACAAAGATTCTCGCTTGATTCGCAAGGCTTCTCAGCCGCCGCCGCAGCTCCGGCAGCCGGTCATAATCTACACCCATTCCCCGAAAATCATCCACACGCAGCCCAAGGATTTCATGGCCCTCGTCCAACGCCTCACCGGCTTCACGCCCTCCGACGCCGCCGCCCCAGCTCAACAAACGCTTCAGAAACCGCCCTCCGACTCCGACAGGAATCTCAACGACAATGATTCTTCCTCGGTTCTCACGGACGACGACAATAATAACGACGGCCGTCGTCCTCACAGAGATTGTAATTCGTCGGCTTTGTCCGATGTTCCTCTGTTCACTCCTCGTTCTTCGCTTCTCAGATATTCTTATTCCGATACGGCGCCTTTTTCGTCTCCTGGTTTAGTTAACTCCTTGTCCCCTTCTTTCATGGAGTTTCTCAAAGCCCTCCCTGAATTTTAA

Coding sequence (CDS)

ATGAGTCCAGCTAAATCCTGCGACCTCAATGGCCCTCGCCCGTCTCCGCTCCTAATTCACAAAGATTCTCGCTTGATTCGCAAGGCTTCTCAGCCGCCGCCGCAGCTCCGGCAGCCGGTCATAATCTACACCCATTCCCCGAAAATCATCCACACGCAGCCCAAGGATTTCATGGCCCTCGTCCAACGCCTCACCGGCTTCACGCCCTCCGACGCCGCCGCCCCAGCTCAACAAACGCTTCAGAAACCGCCCTCCGACTCCGACAGGAATCTCAACGACAATGATTCTTCCTCGGTTCTCACGGACGACGACAATAATAACGACGGCCGTCGTCCTCACAGAGATTGTAATTCGTCGGCTTTGTCCGATGTTCCTCTGTTCACTCCTCGTTCTTCGCTTCTCAGATATTCTTATTCCGATACGGCGCCTTTTTCGTCTCCTGGTTTAGTTAACTCCTTGTCCCCTTCTTTCATGGAGTTTCTCAAAGCCCTCCCTGAATTTTAA

Protein sequence

MSPAKSCDLNGPRPSPLLIHKDSRLIRKASQPPPQLRQPVIIYTHSPKIIHTQPKDFMALVQRLTGFTPSDAAAPAQQTLQKPPSDSDRNLNDNDSSSVLTDDDNNNDGRRPHRDCNSSALSDVPLFTPRSSLLRYSYSDTAPFSSPGLVNSLSPSFMEFLKALPEF
Homology
[Close]
BLAST of Lag0040302 vs. NCBI nr
Match: XP_022150646.1 (VQ motif-containing protein 8, chloroplastic [Momordica charantia])

HSP 1 Score: 184.9 bits (468), Expect = 5.7e-43
Identity = 116/180 (64.44%), Postives = 125/180 (69.44%), Query Frame = 0 

Query: 1   MSPAKSCDLNGPRPSPLLIHKDSRLIRKASQPPPQLRQPVIIYTHSPKIIHTQPKDFMAL 60
           MSPAKSCD+NGPRPSPLLIHK SRLIRK   PPPQLRQP+IIYTHSPKIIHTQPKDFMAL
Sbjct: 1   MSPAKSCDINGPRPSPLLIHKHSRLIRK---PPPQLRQPLIIYTHSPKIIHTQPKDFMAL 60

Query: 61  VQRLTGFTPSDAAAPAQQTLQKPP--SDSDRNLN---DNDSSSVLTDDDNNNDGRR---- 120
           VQRLTGFTP             PP   DSDRN N   DNDSSSVLT++ N  D       
Sbjct: 61  VQRLTGFTP-----------PPPPHADDSDRNNNVNFDNDSSSVLTEEVNCGDHNHHPSY 120

Query: 121 ----PHRDCNSSALSDVPLFTPRSSLLRYSYSDTAPFSSPGLVNSLSPSFMEFLKALPEF 168
                 +  + S L+DVPLFTP    LRY+  D   F+SPGL NSLSPSFMEFLK LPEF
Sbjct: 121 VKEVQSQSQSHSFLADVPLFTPAG--LRYA-PDATSFASPGLPNSLSPSFMEFLKGLPEF 163

[Close]
BLAST of Lag0040302 vs. NCBI nr
Match: XP_038893302.1 (VQ motif-containing protein 8, chloroplastic [Benincasa hispida])

HSP 1 Score: 180.3 bits (456), Expect = 1.4e-41
Identity = 107/167 (64.07%), Postives = 117/167 (70.06%), Query Frame = 0 

Query: 1   MSPAKSCDLNGPRPSPLLIHKDSRLIRKASQPPPQLRQPVIIYTHSPKIIHTQPKDFMAL 60
           MSP KSC++NGPRPSPLLIHKDSR IRK     PQLRQPVIIYTHSPKIIHTQPKDFMAL
Sbjct: 1   MSPPKSCEINGPRPSPLLIHKDSRFIRK-----PQLRQPVIIYTHSPKIIHTQPKDFMAL 60

Query: 61  VQRLTGFTPSDAAAPAQQTLQKPPSDSDRNLNDNDSSSVLTDDDNNNDGRRPHRDCNSSA 120
           VQRLTGF P     P+        S S+RN NDNDSSS LT ++  N+     +   S  
Sbjct: 61  VQRLTGFNPPPPPPPSNAL---SLSFSERNFNDNDSSSGLTTEEEINECGDQKQ---SVV 120

Query: 121 LSDVPLFTPRSSLLRYSYSDTAPFSSPGLVNSLSPSFMEFLKALPEF 168
           L  +      SSLLRYS   T PFSSP L+NSLSPSFMEFLKALPEF
Sbjct: 121 LKQLVQRQSNSSLLRYSSDVTTPFSSPALINSLSPSFMEFLKALPEF 156

[Close]
BLAST of Lag0040302 vs. NCBI nr
Match: XP_008466850.1 (PREDICTED: VQ motif-containing protein 8, chloroplastic-like [Cucumis melo] >KAA0034563.1 VQ motif-containing protein 8 [Cucumis melo var. makuwa] >TYK09116.1 VQ motif-containing protein 8 [Cucumis melo var. makuwa])

HSP 1 Score: 153.7 bits (387), Expect = 1.4e-33
Identity = 91/167 (54.49%), Postives = 107/167 (64.07%), Query Frame = 0 

Query: 1   MSPAKSCDLNGPRPSPLLIHKDSRLIRKASQPPPQLRQPVIIYTHSPKIIHTQPKDFMAL 60
           MSP KS  +NG RPSPLLIH DSRL+RK     PQLRQP+IIYTHSPKIIHT PKDFMAL
Sbjct: 1   MSPPKSSQINGLRPSPLLIHNDSRLVRK-----PQLRQPLIIYTHSPKIIHTHPKDFMAL 60

Query: 61  VQRLTGFTPSDAAAPAQQTLQKPPSDSDRNLNDNDSSSVLTDDDNNNDGRRPHRDCNSSA 120
           VQRLTGF P             PP   +RN NDNDS+S +T +++ N+ +          
Sbjct: 61  VQRLTGFNP-------------PPPQPERNSNDNDSTSGVTSEEDVNEKQ---------- 120

Query: 121 LSDVPLFTPRSSLLRYSYSDTAPFSSPGLVNSLSPSFMEFLKALPEF 168
                      S+L+YS   T PFSSP  ++SLSPSFM+FLKALPEF
Sbjct: 121 --------SNCSVLKYSSDVTTPFSSPAFLSSLSPSFMDFLKALPEF 131

[Close]
BLAST of Lag0040302 vs. NCBI nr
Match: KAG7013019.1 (VQ motif-containing protein 8, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 146.0 bits (367), Expect = 3.0e-31
Identity = 93/167 (55.69%), Postives = 104/167 (62.28%), Query Frame = 0 

Query: 1   MSPAKSCDLNGPRPSPLLIHKDSRLIRKASQPPPQLRQPVIIYTHSPKIIHTQPKDFMAL 60
           MSP K   ++GPRP PLL+HK SR IRK S PPPQLR+PVIIYTHSPKIIHTQPKDFMAL
Sbjct: 1   MSPPKPRHIHGPRPPPLLLHKHSRTIRKPS-PPPQLRRPVIIYTHSPKIIHTQPKDFMAL 60

Query: 61  VQRLTGFTPSDAAAPAQQTLQKPPSDSDRNLNDNDSSSVLTDDDNNNDGRRPHRDCNSSA 120
           VQRLTG +P +A         K PSD      +NDSSSV+T++D+N              
Sbjct: 61  VQRLTGCSPPNA--------DKAPSD------NNDSSSVVTEEDSN-------------- 118

Query: 121 LSDVPLFTPRSSLLRYSYSDTAPFSSPGLVNSLSPSFMEFLKALPEF 168
               PLFTPRS                 LVNSLSPSFMEFLK LPEF
Sbjct: 121 ---CPLFTPRS-----------------LVNSLSPSFMEFLKGLPEF 118

[Close]
BLAST of Lag0040302 vs. NCBI nr
Match: XP_027096313.1 (VQ motif-containing protein 8, chloroplastic-like [Coffea arabica])

HSP 1 Score: 128.3 bits (321), Expect = 6.4e-26
Identity = 88/195 (45.13%), Postives = 113/195 (57.95%), Query Frame = 0 

Query: 9   LNGPRPSPLLIHKDSRLIRKASQ----------------PPPQLRQPVIIYTHSPKIIHT 68
           +NGPRPSPL I+KDS +I+K S                    Q RQPVIIYTHSPKIIHT
Sbjct: 15  INGPRPSPLRINKDSHVIQKPSSMNQVYKKAPKEQNTRLSTQQPRQPVIIYTHSPKIIHT 74

Query: 69  QPKDFMALVQRLTGFTPSDAAAPAQQTLQKPPSDSDRNL----NDNDSSSVLTDDDNNND 128
           +PKDFMALVQ+LTGF+ S+     +Q ++      D+ +     DNDSSSV +DD    D
Sbjct: 75  KPKDFMALVQKLTGFSRSE-----EQIVEAESKRDDQTITISQEDNDSSSVTSDDKYEGD 134

Query: 129 GRRPHRDCNSSA-----------LSDVPLFTPRSSLLR-----YSYSDTAPFSSPGLVNS 168
                   +SSA           L+D+PLFTP S+L       + Y D   F+SP +V+S
Sbjct: 135 NASDVIKESSSAVSPVNKGLNPYLTDIPLFTPNSNLFSSPQPVFRYPDVV-FTSPNIVSS 194

[Close]
BLAST of Lag0040302 vs. ExPASy Swiss-Prot
Match: Q9CA36 (VQ motif-containing protein 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=VQ8 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 3.8e-13
Identity = 60/164 (36.59%), Postives = 85/164 (51.83%), Query Frame = 0 

Query: 1   MSPAKSCDLNGPRPSPLLIHKDSRLIRKA----SQPPPQLR-QPVIIYTHSPKIIHTQPK 60
           M P +  ++NG RPS L +  +S  I+K     S+P P  R  PVIIY HSPK+IHT+ +
Sbjct: 1   MIPTRCNEINGSRPSSLKLAGESHTIKKTSSCKSKPRPHGRASPVIIYAHSPKVIHTRAE 60

Query: 61  DFMALVQRLTGFTPSDAAAPAQQTLQKPPSDSDRNLNDNDSSSVLTDD----DNNNDGRR 120
           DFMALVQRLTG           + +++  S+S        SSSV+T++    D+N     
Sbjct: 61  DFMALVQRLTGL---------DEIIRRNTSES--------SSSVVTEEVNVGDDNTAAPF 120

Query: 121 PHRDCNSSALSDVPLFTPRSSLLRYSYSDTAPFSSPGLVNSLSP 156
                    L+D+PLFTP SS+  +       + SP   +S  P
Sbjct: 121 SQDRTQRQKLTDMPLFTP-SSMTLFGSPTQLMYMSPNRTDSFRP 146

[Close]
BLAST of Lag0040302 vs. ExPASy Swiss-Prot
Match: Q8LGD5 (Protein MKS1 OS=Arabidopsis thaliana OX=3702 GN=MKS1 PE=1 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 2.3e-10
Identity = 34/69 (49.28%), Postives = 44/69 (63.77%), Query Frame = 0 

Query: 11 GPRPSPLLIHKDSRLIRK----------ASQPPPQL-RQPVIIYTHSPKIIHTQPKDFMA 69
          GPRPSPL +HKDS  I+K            QPPP + R+PV+IY  SPK++H    +FM 
Sbjct: 26 GPRPSPLSVHKDSHKIKKPPKHPAPPPNRDQPPPYIPREPVVIYAVSPKVVHATASEFMN 85

[Close]
BLAST of Lag0040302 vs. ExPASy Swiss-Prot
Match: Q9LS54 (VQ motif-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=VQ20 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.5e-09
Identity = 53/156 (33.97%), Postives = 70/156 (44.87%), Query Frame = 0 

Query: 12  PRPSPLLIHKDSRLIRKASQP-----PPQLRQPVIIYTHSPKIIHTQPKDFMALVQRLTG 71
           P P  L ++KDS +I+K   P       + R PVIIYTH+P+IIHT PKDFMALVQ+LTG
Sbjct: 41  PSPPTLKVNKDSHVIKKPPSPSSFSSAAKPRHPVIIYTHTPRIIHTNPKDFMALVQKLTG 100

Query: 72  FTPSD-------------AAAPAQQTLQKPPSDSDRNLN--------------------- 120
            T SD                   +T+ +P  D   N N                     
Sbjct: 101 MTHSDEDLGGGNAMTDPGVVKSINRTVSEPTVDRKNNRNRCGGGNNYLRNYSGAGNGKGI 160

[Close]
BLAST of Lag0040302 vs. ExPASy Swiss-Prot
Match: F4HWF9 (Nuclear speckle RNA-binding protein B OS=Arabidopsis thaliana OX=3702 GN=NSRB PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.8e-07
Identity = 38/96 (39.58%), Postives = 52/96 (54.17%), Query Frame = 0 

Query: 11  GPRPSPLLIHKDS-RLIRKA------SQP-PPQLRQ-----------PVIIYTHSPKIIH 70
           GP+P+PL +  DS ++I+K        QP PPQ  Q           PV IYT +P+IIH
Sbjct: 13  GPKPTPLKVRGDSHKIIKKPPLAPPHPQPQPPQTHQQEPSQSRPPPGPVNIYTVTPRIIH 72

Query: 71  TQPKDFMALVQRLTGFTPSDAAAPAQQTLQKPPSDS 88
           T P +FM LVQRLTG T +   + +  +    P D+
Sbjct: 73  THPNNFMTLVQRLTGQTSTSTTSSSSSSSTSEPKDT 108

The following BLAST results are available for this feature:
BLAST of Lag0040302 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022150646.15.7e-4364.44VQ motif-containing protein 8, chloroplastic [Momordica charantia][more]
XP_038893302.11.4e-4164.07VQ motif-containing protein 8, chloroplastic [Benincasa hispida][more]
XP_008466850.11.4e-3354.49PREDICTED: VQ motif-containing protein 8, chloroplastic-like [Cucumis melo] >KAA... [more]
KAG7013019.13.0e-3155.69VQ motif-containing protein 8, chloroplastic, partial [Cucurbita argyrosperma su... [more]
XP_027096313.16.4e-2645.13VQ motif-containing protein 8, chloroplastic-like [Coffea arabica][more]
BLAST of Lag0040302 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9CA363.8e-1336.59VQ motif-containing protein 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8LGD52.3e-1049.28Protein MKS1 OS=Arabidopsis thaliana OX=3702 GN=MKS1 PE=1 SV=2[more]
Q9LS542.5e-0933.97VQ motif-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=VQ20 PE=2 SV=1[more]
F4HWF91.8e-0739.58Nuclear speckle RNA-binding protein B OS=Arabidopsis thaliana OX=3702 GN=NSRB PE... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008889VQPFAMPF05678VQcoord: 44..68
e-value: 4.9E-11
score: 41.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..103
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..122
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..38
IPR039833VQ motif-containing protein 8, chloroplasticPANTHERPTHR33143:SF42VQ MOTIF-CONTAINING PROTEIN 8, CHLOROPLASTICcoord: 9..167
IPR039607VQ motif-containing protein 8/17/18/20/21/25PANTHERPTHR33143F16F4.1 PROTEIN-RELATEDcoord: 9..167

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040302.1Lag0040302.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
cellular_component GO:0070545 PeBoW complex
cellular_component GO:0030687 preribosome, large subunit precursor
molecular_function GO:0043021 ribonucleoprotein complex binding