Lag0040271 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040271
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAlpha/beta-Hydrolases superfamily protein
Locationchr13: 3460645 .. 3461685 (+)
RNA-Seq ExpressionLag0040271
SyntenyLag0040271
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTACCATTAACCTAGATCAGATTCCAAGTTTTGAAGAACATGGTAATGGCTTTGATCAACATGATGTGTTGGTTGAGGAAATTGAAGGCCTCATTAGAGTTTACGGAAATGGGTATGTGGAAAGAGCACAGATAGTTCCCTGTGTCTCTAACGCATTGCCACCAGAGTTCGGTGTTACTTCTTGGGATGTAGTTGTGGATAAGCTGAACAACATTTGGGCTCGTTTCTATATTCCACAACAGTGCCAGGAGAAGCTGCCTTTGCTTGTATATTTCCATGGAGGTGGATTTTGTGTCGGCTCGGCTGCTTGGAGTTGCTACCATGAGTTCCTGGCTAAGCTATCTGCCAAAGCAGACTGTATAATTATGTCCGTGAATTATCGTTTGGCTCCAGAAAACCCTCTTCCTGCTCCTTATGAAGATGGATTAAAGACCCTTTCATGGTTGAAACAAGTTGCTCTTGTTGGGGCCAAGCAGAATTGGTGGTCAAGATACTGTGACTTTACCAGAATCTACCTTGGTGGTGATAGTGCCGGTGGAAACATAGCCTTCAATGTAGCCACGAGACTTGGTGGTAAGACGACTGCTTCTGGAGCTGTGGTCTTGAAACCGTTAGTTATTAAAGGTAACATTTTGATACAACCATTTTTTAGTGGGGAGTCAAGGACCAAGTCAGAGAAGTTTCTTGTCCAACCTCCTCGTTCGCCACTGACGATGGCAGTTTCTGATACATACTGGCGGTTGGCGTTGCCTTCTGGAGCAAACCGCGACCACCCATGGTGCAACCCTTCGGCAAAAGGGTTGTTTAAGGTGGAGGATTTGAGGGTTTTGCCTAGTTTGGTATGCATATCAGAAATGGACATATTGAAAGATAGGAACTTGGAGTTTTGCTCTGCCTTACATAGAGAGGGTAAGCTGATCAATTATGTGGTTTATGAAGGAGTCGGCCATGCGTTTCAGGTTCTGAACAAGTCACAACTCTCACAGACTCGGACCCTTGAAATGATTGATCATATCAAGGATTTCTTAAGTGTATAG

mRNA sequence

ATGCCTACCATTAACCTAGATCAGATTCCAAGTTTTGAAGAACATGGTAATGGCTTTGATCAACATGATGTGTTGGTTGAGGAAATTGAAGGCCTCATTAGAGTTTACGGAAATGGGTATGTGGAAAGAGCACAGATAGTTCCCTGTGTCTCTAACGCATTGCCACCAGAGTTCGGTGTTACTTCTTGGGATGTAGTTGTGGATAAGCTGAACAACATTTGGGCTCGTTTCTATATTCCACAACAGTGCCAGGAGAAGCTGCCTTTGCTTGTATATTTCCATGGAGGTGGATTTTGTGTCGGCTCGGCTGCTTGGAGTTGCTACCATGAGTTCCTGGCTAAGCTATCTGCCAAAGCAGACTGTATAATTATGTCCGTGAATTATCGTTTGGCTCCAGAAAACCCTCTTCCTGCTCCTTATGAAGATGGATTAAAGACCCTTTCATGGTTGAAACAAGTTGCTCTTGTTGGGGCCAAGCAGAATTGGTGGTCAAGATACTGTGACTTTACCAGAATCTACCTTGGTGGTGATAGTGCCGGTGGAAACATAGCCTTCAATGTAGCCACGAGACTTGGTGGTAAGACGACTGCTTCTGGAGCTGTGGTCTTGAAACCGTTAGTTATTAAAGGTAACATTTTGATACAACCATTTTTTAGTGGGGAGTCAAGGACCAAGTCAGAGAAGTTTCTTGTCCAACCTCCTCGTTCGCCACTGACGATGGCAGTTTCTGATACATACTGGCGGTTGGCGTTGCCTTCTGGAGCAAACCGCGACCACCCATGGTGCAACCCTTCGGCAAAAGGGTTGTTTAAGGTGGAGGATTTGAGGGTTTTGCCTAGTTTGGTATGCATATCAGAAATGGACATATTGAAAGATAGGAACTTGGAGTTTTGCTCTGCCTTACATAGAGAGGGTAAGCTGATCAATTATGTGGTTTATGAAGGAGTCGGCCATGCGTTTCAGGTTCTGAACAAGTCACAACTCTCACAGACTCGGACCCTTGAAATGATTGATCATATCAAGGATTTCTTAAGTGTATAG

Coding sequence (CDS)

ATGCCTACCATTAACCTAGATCAGATTCCAAGTTTTGAAGAACATGGTAATGGCTTTGATCAACATGATGTGTTGGTTGAGGAAATTGAAGGCCTCATTAGAGTTTACGGAAATGGGTATGTGGAAAGAGCACAGATAGTTCCCTGTGTCTCTAACGCATTGCCACCAGAGTTCGGTGTTACTTCTTGGGATGTAGTTGTGGATAAGCTGAACAACATTTGGGCTCGTTTCTATATTCCACAACAGTGCCAGGAGAAGCTGCCTTTGCTTGTATATTTCCATGGAGGTGGATTTTGTGTCGGCTCGGCTGCTTGGAGTTGCTACCATGAGTTCCTGGCTAAGCTATCTGCCAAAGCAGACTGTATAATTATGTCCGTGAATTATCGTTTGGCTCCAGAAAACCCTCTTCCTGCTCCTTATGAAGATGGATTAAAGACCCTTTCATGGTTGAAACAAGTTGCTCTTGTTGGGGCCAAGCAGAATTGGTGGTCAAGATACTGTGACTTTACCAGAATCTACCTTGGTGGTGATAGTGCCGGTGGAAACATAGCCTTCAATGTAGCCACGAGACTTGGTGGTAAGACGACTGCTTCTGGAGCTGTGGTCTTGAAACCGTTAGTTATTAAAGGTAACATTTTGATACAACCATTTTTTAGTGGGGAGTCAAGGACCAAGTCAGAGAAGTTTCTTGTCCAACCTCCTCGTTCGCCACTGACGATGGCAGTTTCTGATACATACTGGCGGTTGGCGTTGCCTTCTGGAGCAAACCGCGACCACCCATGGTGCAACCCTTCGGCAAAAGGGTTGTTTAAGGTGGAGGATTTGAGGGTTTTGCCTAGTTTGGTATGCATATCAGAAATGGACATATTGAAAGATAGGAACTTGGAGTTTTGCTCTGCCTTACATAGAGAGGGTAAGCTGATCAATTATGTGGTTTATGAAGGAGTCGGCCATGCGTTTCAGGTTCTGAACAAGTCACAACTCTCACAGACTCGGACCCTTGAAATGATTGATCATATCAAGGATTTCTTAAGTGTATAG

Protein sequence

MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGVTSWDVVVDKLNNIWARFYIPQQCQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGGDSAGGNIAFNVATRLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTMAVSDTYWRLALPSGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSALHREGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLSV
Homology
BLAST of Lag0040271 vs. NCBI nr
Match: XP_038893358.1 (probable carboxylesterase 6 [Benincasa hispida] >XP_038893359.1 probable carboxylesterase 6 [Benincasa hispida] >XP_038893360.1 probable carboxylesterase 6 [Benincasa hispida] >XP_038893361.1 probable carboxylesterase 6 [Benincasa hispida])

HSP 1 Score: 697.6 bits (1799), Expect = 5.5e-197
Identity = 329/346 (95.09%), Postives = 335/346 (96.82%), Query Frame = 0

Query: 1   MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGV 60
           MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVER QIVPCVSNALPPE GV
Sbjct: 6   MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 65

Query: 61  TSWDVVVDKLNNIWARFYIPQQCQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 120
           TSWDVV+DKLNNIWARFYIP QCQEKLPL+VYFHGGGFCVGSAAWSCYHEFLAKLSAKAD
Sbjct: 66  TSWDVVMDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 125

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKTL WLKQVA VGAKQNWWSRYCDFT+IYL GDSAG
Sbjct: 126 CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLSGDSAG 185

Query: 181 GNIAFNVATRLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTM 240
           GNIAFNVA RLGGKTTASGAVVLKPLVIKG+ILIQPFF GESRTKSEKFLVQPPRSPLT+
Sbjct: 186 GNIAFNVAARLGGKTTASGAVVLKPLVIKGSILIQPFFGGESRTKSEKFLVQPPRSPLTL 245

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSA 300
           AVSDTYWRLALPSG NRDHPWCNPSAKGLFKVEDLRVLPSL+CISEMDILKDRNLEFCSA
Sbjct: 246 AVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISEMDILKDRNLEFCSA 305

Query: 301 LHREGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLSV 347
           LHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFL V
Sbjct: 306 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLCV 351

BLAST of Lag0040271 vs. NCBI nr
Match: XP_022944859.1 (probable carboxylesterase 6 [Cucurbita moschata] >KAG7013040.1 putative carboxylesterase 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 686.0 bits (1769), Expect = 1.6e-193
Identity = 325/346 (93.93%), Postives = 336/346 (97.11%), Query Frame = 0

Query: 1   MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGV 60
           MPTINL QIPSFEEH NGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPE GV
Sbjct: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60

Query: 61  TSWDVVVDKLNNIWARFYIPQQCQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 120
           TSWDVVV+KL+NIWARFYIP+QCQ KLPLLVYFHGGGFCVGSAAWSCYHEFLAK+SAKA+
Sbjct: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKT+SWLKQVAL+G KQNWWSRYCDFTRIYLGGDSAG
Sbjct: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180

Query: 181 GNIAFNVATRLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTM 240
           GNIAFNVA RLGGKTTASGAVVLKPLVI+GNILIQPFF GESRTKSEKFLVQPPRS LTM
Sbjct: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSA 300
           AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVE+LRVLPSLVCISE+DILKDRNLEFCSA
Sbjct: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300

Query: 301 LHREGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLSV 347
           LHREGKLINYVV+EGVGHAFQVLNKSQLSQTRTLEMI HIKDFL V
Sbjct: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 346

BLAST of Lag0040271 vs. NCBI nr
Match: KAG6573982.1 (putative carboxylesterase 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 684.9 bits (1766), Expect = 3.7e-193
Identity = 324/346 (93.64%), Postives = 336/346 (97.11%), Query Frame = 0

Query: 1   MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGV 60
           MPTINL QIPSFEEH NGFDQHDVLVEEIEGLIRVYGNGYV+RAQIVPCVSNALPPE GV
Sbjct: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVDRAQIVPCVSNALPPELGV 60

Query: 61  TSWDVVVDKLNNIWARFYIPQQCQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 120
           TSWDVVV+KL+NIWARFYIP+QCQ KLPLLVYFHGGGFCVGSAAWSCYHEFLAK+SAKA+
Sbjct: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKT+SWLKQVAL+G KQNWWSRYCDFTRIYLGGDSAG
Sbjct: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180

Query: 181 GNIAFNVATRLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTM 240
           GNIAFNVA RLGGKTTASGAVVLKPLVI+GNILIQPFF GESRTKSEKFLVQPPRS LTM
Sbjct: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSA 300
           AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVE+LRVLPSLVCISE+DILKDRNLEFCSA
Sbjct: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300

Query: 301 LHREGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLSV 347
           LHREGKLINYVV+EGVGHAFQVLNKSQLSQTRTLEMI HIKDFL V
Sbjct: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 346

BLAST of Lag0040271 vs. NCBI nr
Match: XP_004135407.1 (probable carboxylesterase 6 [Cucumis sativus] >XP_011655765.1 probable carboxylesterase 6 [Cucumis sativus] >XP_031741302.1 probable carboxylesterase 6 [Cucumis sativus] >XP_031741303.1 probable carboxylesterase 6 [Cucumis sativus] >KGN52062.1 hypothetical protein Csa_008345 [Cucumis sativus])

HSP 1 Score: 682.9 bits (1761), Expect = 1.4e-192
Identity = 322/346 (93.06%), Postives = 329/346 (95.09%), Query Frame = 0

Query: 1   MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGV 60
           MPTINLDQIPSFEEH NGFDQHDVLVEEIEGLIRVYGNGYVER QIVPCVSNALPPE GV
Sbjct: 6   MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 65

Query: 61  TSWDVVVDKLNNIWARFYIPQQCQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 120
           TSWDVVVDKLNNIWARFYIP QCQEKLPL+VYFHGGGFCVGSAAWSCYHEFLAKLSAKA+
Sbjct: 66  TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKAN 125

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKTL WLKQVA VG KQNWWSRYCDFT+IYL GDSAG
Sbjct: 126 CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG 185

Query: 181 GNIAFNVATRLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTM 240
           GNIAFNVA RLGGKTTASGAV+LKPLVIKG+ILIQPFF GESRTKSEKFLVQPPRSPLT+
Sbjct: 186 GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPRSPLTL 245

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSA 300
            VSDTYWRLALPSG NRDHPWCNPS KGLF VEDLRVLPSL+CISEMDILKDRNLEFCSA
Sbjct: 246 GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNLEFCSA 305

Query: 301 LHREGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLSV 347
           LHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIK FL V
Sbjct: 306 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351

BLAST of Lag0040271 vs. NCBI nr
Match: XP_022968602.1 (probable carboxylesterase 6 [Cucurbita maxima])

HSP 1 Score: 682.6 bits (1760), Expect = 1.8e-192
Identity = 323/346 (93.35%), Postives = 335/346 (96.82%), Query Frame = 0

Query: 1   MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGV 60
           MPTINL QIPSFEEH NGFDQHD+LVEEIEGLIRVYGNGYVERAQIVPCVSNALPPE GV
Sbjct: 1   MPTINLGQIPSFEEHVNGFDQHDMLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60

Query: 61  TSWDVVVDKLNNIWARFYIPQQCQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 120
           TSWDVVV+KL+NIWARFYIP+QCQ KLPLLVYFHGGGFCVGSAAWSCYHEFLAK+SAKA+
Sbjct: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKT+SWLKQVAL+G KQNWWSRYCDFTRIYLGGDSAG
Sbjct: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180

Query: 181 GNIAFNVATRLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTM 240
           GNIAFNVA RLGGKTTASGAVVLKPLVI+GNILIQPFF GESRTKSEKFLVQPPRS LTM
Sbjct: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSA 300
           AVSDTYWRLALP GANRDHPWCNPSAKGLFKVE+LRVLPSLVCISE+DILKDRNLEFCSA
Sbjct: 241 AVSDTYWRLALPFGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300

Query: 301 LHREGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLSV 347
           LHREGKLINYVV+EGVGHAFQVLNKSQLSQTRTLEMI HIKDFL V
Sbjct: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 346

BLAST of Lag0040271 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.5e-96
Identity = 172/332 (51.81%), Postives = 228/332 (68.67%), Query Frame = 0

Query: 17  NGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGVTSWDVVVDKLNNIWAR 76
           N  + H  +V+E+EGLI+VY +G+VER+Q++PCV  +LP E GVT  DVV+DKL N+WAR
Sbjct: 15  NNSNIHGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPLELGVTCSDVVIDKLTNVWAR 74

Query: 77  FYIP----QQCQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAP 136
            Y+P    +    KLPL+VYFHGGGFCVGSA+W CYHEFLA+LSA++ C++MSVNYRLAP
Sbjct: 75  LYVPMTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAP 134

Query: 137 ENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGGDSAGGNIAFNVATRLG 196
           ENPLPA YEDG+  + WL +        N W++ CDF RI+L GDSAGGNIA  VA RL 
Sbjct: 135 ENPLPAAYEDGVNAILWLNK----ARNDNLWAKQCDFGRIFLAGDSAGGNIAQQVAARLA 194

Query: 197 GKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTMAVSDTYWRLALP 256
                +       L I+G ILIQPF+SGE RT+SE+ +     + LT+A SD +WR++LP
Sbjct: 195 SPEDLA-------LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLP 254

Query: 257 SGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSALHREGKLINYVV 316
            GANR+HP+C P       ++   V  +LVC++EMD+L D N+E C        +I  V+
Sbjct: 255 RGANREHPYCKPVK---MIIKSSTVTRTLVCVAEMDLLMDSNMEMCDG---NEDVIKRVL 314

Query: 317 YEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFL 345
           ++GVGHAF +L KSQL+ T TLEM+  I  F+
Sbjct: 315 HKGVGHAFHILGKSQLAHTTTLEMLCQIDAFI 329

BLAST of Lag0040271 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.4e-86
Identity = 163/335 (48.66%), Postives = 218/335 (65.07%), Query Frame = 0

Query: 16  GNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGVTSWDVVVDKLNNIWA 75
           G+   +H  +VEEIEGLI+V+ +G VER  IVP VS  + P    T++D+ +   N+ W 
Sbjct: 19  GSHHHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFDIKLS--NDTWT 78

Query: 76  RFYIPQQCQEK----LPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLA 135
           R YIP          LPLLVYFHGGGFCVGSAAWSCYH+FL  L+ KA C+I+SVNYRLA
Sbjct: 79  RVYIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLA 138

Query: 136 PENPLPAPYEDGLKTLSWL--KQVALVGAKQNWWSRYCDFTRIYLGGDSAGGNIAFNVAT 195
           PE+ LPA Y+DG+  +SWL  +Q++  G   +W S+ C+ + ++L GDSAG NIA+ VA 
Sbjct: 139 PEHRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSK-CNLSNVFLAGDSAGANIAYQVAV 198

Query: 196 RLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTMAVSDTYWRL 255
           R+     ASG      L +KG ILI PFF GESRT SEK       S LT++ SD YWRL
Sbjct: 199 RI----MASGKYA-NTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRL 258

Query: 256 ALPSGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSALHREGKLIN 315
           ALP GA+RDHPWCNP    L      ++  ++V ++E DILK+RNLE C  +   GK + 
Sbjct: 259 ALPRGASRDHPWCNP----LMSSAGAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVE 318

Query: 316 YVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFL 345
            +V+ GVGHAF +L+ S +S+ R  +M+  + +F+
Sbjct: 319 GIVHGGVGHAFHILDNSSVSRDRIHDMMCRLHNFI 341

BLAST of Lag0040271 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.3e-52
Identity = 113/328 (34.45%), Postives = 182/328 (55.49%), Query Frame = 0

Query: 25  LVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGVTSW--DVVVDKLNNIWARFYIPQQ 84
           + E+  GL+++  NG V R++ +  ++  +P +   T    D +  K NN+  R Y P  
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 85  CQEK--LPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPY 144
              +  LP++V+FHGGGFC GS +W  +H F   L++  + +++S +YRLAPE+ LPA +
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 145 EDGLKTLSWLKQVALVGAKQNWW--SRYCDFTRIYLGGDSAGGNIAFNVATRLGGKTTAS 204
           ED    L+WL   A+     +W+      DF R+++ GDS+GGNIA  +A R G     S
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-----S 189

Query: 205 GAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTMAVSDTYWRLALPSGANRD 264
           G++ L P+ ++G +L+ PFF GE RT SE     P  + L++ + D +WRL+LP+GA RD
Sbjct: 190 GSIELTPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRD 249

Query: 265 HPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSALHR-EGKLINYVVYEGVG 324
           H   NP       +E + + P LV +   ++L+DR  E+   L +  GK ++Y+ +E   
Sbjct: 250 HHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKE 309

Query: 325 HAFQVLNKSQLSQTRTLEMIDHIKDFLS 346
           H F     S  +  + L +I    + LS
Sbjct: 310 HGFYSNYPSSEAAEQVLRIIGDFMNNLS 329

BLAST of Lag0040271 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.5e-43
Identity = 103/280 (36.79%), Postives = 159/280 (56.79%), Query Frame = 0

Query: 60  VTSWDVVVDKLNNIWARFYIPQQC---QEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLS 119
           V + D+ ++ L+N + R ++P+       KLPL+VYFHGGGF + SAA + +H+F  +++
Sbjct: 53  VLTKDLALNPLHNTFVRLFLPRHALYNSAKLPLVVYFHGGGFILFSAASTIFHDFCCEMA 112

Query: 120 AKADCIIMSVNYRLAPENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGG 179
             A  +I SV+YRLAPE+ LPA Y+D ++ L W+K      ++  W + + DF+  ++ G
Sbjct: 113 VHAGVVIASVDYRLAPEHRLPAAYDDAMEALQWIKD-----SRDEWLTNFADFSNCFIMG 172

Query: 180 DSAGGNIAFNVATRLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRS 239
           +SAGGNIA++   R      A+ A  L PL IKG +L +P F G  RT SE  L    R 
Sbjct: 173 ESAGGNIAYHAGLR-----AAAVADELLPLKIKGLVLDEPGFGGSKRTGSELRLANDSRL 232

Query: 240 PLTMAVSDTYWRLALPSGANRDHPWCNPSAKG--LFKVEDLRVLPSLVCI--SEMDILKD 299
           P    V D  W L+LP GA+RDH +CNP+A+   L+  + +R L   V +     D + D
Sbjct: 233 P--TFVLDLIWELSLPMGADRDHEYCNPTAESEPLYSFDKIRSLGWRVMVVGCHGDPMID 292

Query: 300 RNLEFCSALHREGKLINYVVYEGVG--HAFQVLNKSQLSQ 331
           R +E    L ++G  ++ V    VG  HA ++ +  +  Q
Sbjct: 293 RQMELAERLEKKG--VDVVAQFDVGGYHAVKLEDPEKAKQ 318

BLAST of Lag0040271 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.3e-41
Identity = 111/329 (33.74%), Postives = 163/329 (49.54%), Query Frame = 0

Query: 28  EIEGLIRVYGNGYVER---AQIVPCVSNALPPEFGVTSWDVVVDKLNNIWARFYIPQ--Q 87
           E+  L+RVY +G VER   +  VP   +   PE GV++ D+V+ +   I AR Y+P+   
Sbjct: 16  ELPPLLRVYKDGTVERFLGSSFVP--PSPEDPETGVSTKDIVISENPTISARVYLPKLNN 75

Query: 88  CQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPYED 147
             EKLP+LVY+HGG FC+ SA    +  +L  +++KA+ +++S+ YRLAPE+PLPA YED
Sbjct: 76  TTEKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEYRLAPEHPLPAAYED 135

Query: 148 GLKTLSWLKQVALVGAKQN----WWSRYCDFTRIYLGGDSAGGNIAFNVATRLGGKTTAS 207
           G   L W+   +    K      W  ++ DF R Y+GGD++G NIA N A R+G +    
Sbjct: 136 GWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIAHNAALRVGAEALPG 195

Query: 208 GAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTMAVSDTYWRLALPSG-ANR 267
           G      L I G +   P F G     SE        SP+ +      W    P      
Sbjct: 196 G------LRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPMQV------WNFVYPDAPGGI 255

Query: 268 DHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSALHREGKL--INYVVYEG 327
           D+P  NP A G   +  L     LV ++  D L+DR + +  A+   G    +    YEG
Sbjct: 256 DNPLINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEG 315

Query: 328 VGHAFQVLNKSQLSQTRTLEMIDHIKDFL 345
             H FQ+ +        + ++I  I  FL
Sbjct: 316 EEHCFQIYHP---ETENSKDLIGRIASFL 327

BLAST of Lag0040271 vs. ExPASy TrEMBL
Match: A0A6J1FZC1 (probable carboxylesterase 6 OS=Cucurbita moschata OX=3662 GN=LOC111449259 PE=4 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 8.0e-194
Identity = 325/346 (93.93%), Postives = 336/346 (97.11%), Query Frame = 0

Query: 1   MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGV 60
           MPTINL QIPSFEEH NGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPE GV
Sbjct: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60

Query: 61  TSWDVVVDKLNNIWARFYIPQQCQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 120
           TSWDVVV+KL+NIWARFYIP+QCQ KLPLLVYFHGGGFCVGSAAWSCYHEFLAK+SAKA+
Sbjct: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKT+SWLKQVAL+G KQNWWSRYCDFTRIYLGGDSAG
Sbjct: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180

Query: 181 GNIAFNVATRLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTM 240
           GNIAFNVA RLGGKTTASGAVVLKPLVI+GNILIQPFF GESRTKSEKFLVQPPRS LTM
Sbjct: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSA 300
           AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVE+LRVLPSLVCISE+DILKDRNLEFCSA
Sbjct: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300

Query: 301 LHREGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLSV 347
           LHREGKLINYVV+EGVGHAFQVLNKSQLSQTRTLEMI HIKDFL V
Sbjct: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 346

BLAST of Lag0040271 vs. ExPASy TrEMBL
Match: A0A0A0KUG5 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G608500 PE=4 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 6.7e-193
Identity = 322/346 (93.06%), Postives = 329/346 (95.09%), Query Frame = 0

Query: 1   MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGV 60
           MPTINLDQIPSFEEH NGFDQHDVLVEEIEGLIRVYGNGYVER QIVPCVSNALPPE GV
Sbjct: 6   MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 65

Query: 61  TSWDVVVDKLNNIWARFYIPQQCQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 120
           TSWDVVVDKLNNIWARFYIP QCQEKLPL+VYFHGGGFCVGSAAWSCYHEFLAKLSAKA+
Sbjct: 66  TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKAN 125

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKTL WLKQVA VG KQNWWSRYCDFT+IYL GDSAG
Sbjct: 126 CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG 185

Query: 181 GNIAFNVATRLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTM 240
           GNIAFNVA RLGGKTTASGAV+LKPLVIKG+ILIQPFF GESRTKSEKFLVQPPRSPLT+
Sbjct: 186 GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPRSPLTL 245

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSA 300
            VSDTYWRLALPSG NRDHPWCNPS KGLF VEDLRVLPSL+CISEMDILKDRNLEFCSA
Sbjct: 246 GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNLEFCSA 305

Query: 301 LHREGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLSV 347
           LHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIK FL V
Sbjct: 306 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351

BLAST of Lag0040271 vs. ExPASy TrEMBL
Match: A0A6J1HTY6 (probable carboxylesterase 6 OS=Cucurbita maxima OX=3661 GN=LOC111467774 PE=4 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 8.8e-193
Identity = 323/346 (93.35%), Postives = 335/346 (96.82%), Query Frame = 0

Query: 1   MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGV 60
           MPTINL QIPSFEEH NGFDQHD+LVEEIEGLIRVYGNGYVERAQIVPCVSNALPPE GV
Sbjct: 1   MPTINLGQIPSFEEHVNGFDQHDMLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60

Query: 61  TSWDVVVDKLNNIWARFYIPQQCQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 120
           TSWDVVV+KL+NIWARFYIP+QCQ KLPLLVYFHGGGFCVGSAAWSCYHEFLAK+SAKA+
Sbjct: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKT+SWLKQVAL+G KQNWWSRYCDFTRIYLGGDSAG
Sbjct: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180

Query: 181 GNIAFNVATRLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTM 240
           GNIAFNVA RLGGKTTASGAVVLKPLVI+GNILIQPFF GESRTKSEKFLVQPPRS LTM
Sbjct: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSA 300
           AVSDTYWRLALP GANRDHPWCNPSAKGLFKVE+LRVLPSLVCISE+DILKDRNLEFCSA
Sbjct: 241 AVSDTYWRLALPFGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300

Query: 301 LHREGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLSV 347
           LHREGKLINYVV+EGVGHAFQVLNKSQLSQTRTLEMI HIKDFL V
Sbjct: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 346

BLAST of Lag0040271 vs. ExPASy TrEMBL
Match: A0A1S3BFC8 (probable carboxylesterase 6 OS=Cucumis melo OX=3656 GN=LOC103489265 PE=4 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 7.5e-192
Identity = 319/346 (92.20%), Postives = 327/346 (94.51%), Query Frame = 0

Query: 1   MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGV 60
           MPTINLDQIPSFEEH NGFDQHDVLVEEIEGLIRVYGNGYVER QIVPCVSNALPPE GV
Sbjct: 1   MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 60

Query: 61  TSWDVVVDKLNNIWARFYIPQQCQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 120
           TSWDVVVDKLNNIWARFYIP QCQEKLPL+VYFHGGGFCVGSAAWSCYHEFLAKLSAK+D
Sbjct: 61  TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKSD 120

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGGDSAG 180
           CII+SVNYRLAPENPLPAPYEDGLKTL WLKQVA VG KQNWWSRYCDFT+IYL GDSAG
Sbjct: 121 CIIISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG 180

Query: 181 GNIAFNVATRLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTM 240
           GNIAFNVA RLGGKTTASGAV+LKPLVIKG+ILIQPFF GESRT SEKFLVQPPRSPLT+
Sbjct: 181 GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPRSPLTL 240

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSA 300
            VSDTYWRLALPSG NRDHPWCNPS KGLF VED RVLPSL+CISEMDILKDRNLEFCSA
Sbjct: 241 GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNLEFCSA 300

Query: 301 LHREGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLSV 347
           LHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM DHIKDFL V
Sbjct: 301 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFLCV 346

BLAST of Lag0040271 vs. ExPASy TrEMBL
Match: A0A5A7SV76 (Putative carboxylesterase 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00820 PE=4 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 7.5e-192
Identity = 319/346 (92.20%), Postives = 327/346 (94.51%), Query Frame = 0

Query: 1   MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGV 60
           MPTINLDQIPSFEEH NGFDQHDVLVEEIEGLIRVYGNGYVER QIVPCVSNALPPE GV
Sbjct: 6   MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 65

Query: 61  TSWDVVVDKLNNIWARFYIPQQCQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 120
           TSWDVVVDKLNNIWARFYIP QCQEKLPL+VYFHGGGFCVGSAAWSCYHEFLAKLSAK+D
Sbjct: 66  TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKSD 125

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGGDSAG 180
           CII+SVNYRLAPENPLPAPYEDGLKTL WLKQVA VG KQNWWSRYCDFT+IYL GDSAG
Sbjct: 126 CIIISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG 185

Query: 181 GNIAFNVATRLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTM 240
           GNIAFNVA RLGGKTTASGAV+LKPLVIKG+ILIQPFF GESRT SEKFLVQPPRSPLT+
Sbjct: 186 GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPRSPLTL 245

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSA 300
            VSDTYWRLALPSG NRDHPWCNPS KGLF VED RVLPSL+CISEMDILKDRNLEFCSA
Sbjct: 246 GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNLEFCSA 305

Query: 301 LHREGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLSV 347
           LHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM DHIKDFL V
Sbjct: 306 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFLCV 351

BLAST of Lag0040271 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 353.6 bits (906), Expect = 1.8e-97
Identity = 172/332 (51.81%), Postives = 228/332 (68.67%), Query Frame = 0

Query: 17  NGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGVTSWDVVVDKLNNIWAR 76
           N  + H  +V+E+EGLI+VY +G+VER+Q++PCV  +LP E GVT  DVV+DKL N+WAR
Sbjct: 15  NNSNIHGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPLELGVTCSDVVIDKLTNVWAR 74

Query: 77  FYIP----QQCQEKLPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAP 136
            Y+P    +    KLPL+VYFHGGGFCVGSA+W CYHEFLA+LSA++ C++MSVNYRLAP
Sbjct: 75  LYVPMTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAP 134

Query: 137 ENPLPAPYEDGLKTLSWLKQVALVGAKQNWWSRYCDFTRIYLGGDSAGGNIAFNVATRLG 196
           ENPLPA YEDG+  + WL +        N W++ CDF RI+L GDSAGGNIA  VA RL 
Sbjct: 135 ENPLPAAYEDGVNAILWLNK----ARNDNLWAKQCDFGRIFLAGDSAGGNIAQQVAARLA 194

Query: 197 GKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTMAVSDTYWRLALP 256
                +       L I+G ILIQPF+SGE RT+SE+ +     + LT+A SD +WR++LP
Sbjct: 195 SPEDLA-------LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLP 254

Query: 257 SGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSALHREGKLINYVV 316
            GANR+HP+C P       ++   V  +LVC++EMD+L D N+E C        +I  V+
Sbjct: 255 RGANREHPYCKPVK---MIIKSSTVTRTLVCVAEMDLLMDSNMEMCDG---NEDVIKRVL 314

Query: 317 YEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFL 345
           ++GVGHAF +L KSQL+ T TLEM+  I  F+
Sbjct: 315 HKGVGHAFHILGKSQLAHTTTLEMLCQIDAFI 329

BLAST of Lag0040271 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 320.5 bits (820), Expect = 1.7e-87
Identity = 163/335 (48.66%), Postives = 218/335 (65.07%), Query Frame = 0

Query: 16  GNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGVTSWDVVVDKLNNIWA 75
           G+   +H  +VEEIEGLI+V+ +G VER  IVP VS  + P    T++D+ +   N+ W 
Sbjct: 19  GSHHHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFDIKLS--NDTWT 78

Query: 76  RFYIPQQCQEK----LPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLA 135
           R YIP          LPLLVYFHGGGFCVGSAAWSCYH+FL  L+ KA C+I+SVNYRLA
Sbjct: 79  RVYIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLA 138

Query: 136 PENPLPAPYEDGLKTLSWL--KQVALVGAKQNWWSRYCDFTRIYLGGDSAGGNIAFNVAT 195
           PE+ LPA Y+DG+  +SWL  +Q++  G   +W S+ C+ + ++L GDSAG NIA+ VA 
Sbjct: 139 PEHRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSK-CNLSNVFLAGDSAGANIAYQVAV 198

Query: 196 RLGGKTTASGAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTMAVSDTYWRL 255
           R+     ASG      L +KG ILI PFF GESRT SEK       S LT++ SD YWRL
Sbjct: 199 RI----MASGKYA-NTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRL 258

Query: 256 ALPSGANRDHPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSALHREGKLIN 315
           ALP GA+RDHPWCNP    L      ++  ++V ++E DILK+RNLE C  +   GK + 
Sbjct: 259 ALPRGASRDHPWCNP----LMSSAGAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVE 318

Query: 316 YVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFL 345
            +V+ GVGHAF +L+ S +S+ R  +M+  + +F+
Sbjct: 319 GIVHGGVGHAFHILDNSSVSRDRIHDMMCRLHNFI 341

BLAST of Lag0040271 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 208.4 bits (529), Expect = 9.4e-54
Identity = 113/328 (34.45%), Postives = 182/328 (55.49%), Query Frame = 0

Query: 25  LVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGVTSW--DVVVDKLNNIWARFYIPQQ 84
           + E+  GL+++  NG V R++ +  ++  +P +   T    D +  K NN+  R Y P  
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 85  CQEK--LPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPY 144
              +  LP++V+FHGGGFC GS +W  +H F   L++  + +++S +YRLAPE+ LPA +
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 145 EDGLKTLSWLKQVALVGAKQNWW--SRYCDFTRIYLGGDSAGGNIAFNVATRLGGKTTAS 204
           ED    L+WL   A+     +W+      DF R+++ GDS+GGNIA  +A R G     S
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-----S 189

Query: 205 GAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTMAVSDTYWRLALPSGANRD 264
           G++ L P+ ++G +L+ PFF GE RT SE     P  + L++ + D +WRL+LP+GA RD
Sbjct: 190 GSIELTPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRD 249

Query: 265 HPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSALHR-EGKLINYVVYEGVG 324
           H   NP       +E + + P LV +   ++L+DR  E+   L +  GK ++Y+ +E   
Sbjct: 250 HHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKE 309

Query: 325 HAFQVLNKSQLSQTRTLEMIDHIKDFLS 346
           H F     S  +  + L +I    + LS
Sbjct: 310 HGFYSNYPSSEAAEQVLRIIGDFMNNLS 329

BLAST of Lag0040271 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 208.4 bits (529), Expect = 9.4e-54
Identity = 113/328 (34.45%), Postives = 182/328 (55.49%), Query Frame = 0

Query: 25  LVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEFGVTSW--DVVVDKLNNIWARFYIPQQ 84
           + E+  GL+++  NG V R++ +  ++  +P +   T    D +  K NN+  R Y P  
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 85  CQEK--LPLLVYFHGGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPY 144
              +  LP++V+FHGGGFC GS +W  +H F   L++  + +++S +YRLAPE+ LPA +
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 145 EDGLKTLSWLKQVALVGAKQNWW--SRYCDFTRIYLGGDSAGGNIAFNVATRLGGKTTAS 204
           ED    L+WL   A+     +W+      DF R+++ GDS+GGNIA  +A R G     S
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-----S 189

Query: 205 GAVVLKPLVIKGNILIQPFFSGESRTKSEKFLVQPPRSPLTMAVSDTYWRLALPSGANRD 264
           G++ L P+ ++G +L+ PFF GE RT SE     P  + L++ + D +WRL+LP+GA RD
Sbjct: 190 GSIELTPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRD 249

Query: 265 HPWCNPSAKGLFKVEDLRVLPSLVCISEMDILKDRNLEFCSALHR-EGKLINYVVYEGVG 324
           H   NP       +E + + P LV +   ++L+DR  E+   L +  GK ++Y+ +E   
Sbjct: 250 HHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKE 309

Query: 325 HAFQVLNKSQLSQTRTLEMIDHIKDFLS 346
           H F     S  +  + L +I    + LS
Sbjct: 310 HGFYSNYPSSEAAEQVLRIIGDFMNNLS 329

BLAST of Lag0040271 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 9.1e-41
Identity = 98/289 (33.91%), Postives = 151/289 (52.25%), Query Frame = 0

Query: 51  SNALPPEFGVTSWDVVVDKLNNIWARFYIPQQCQEK----------------LPLLVYFH 110
           +NA P + GV S+DV++D+  N+ +R Y P    ++                +P++++FH
Sbjct: 55  ANANPVD-GVFSFDVLIDRRINLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFH 114

Query: 111 GGGFCVGSAAWSCYHEFLAKLSAKADCIIMSVNYRLAPENPLPAPYEDGLKTLSWLKQVA 170
           GG F   SA  + Y     +L     C+++SVNYR APENP P  Y+DG   L+W+   +
Sbjct: 115 GGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSRS 174

Query: 171 LVGAKQNWWSRYCDFTRIYLGGDSAGGNIAFNVATRLGGKTTASGAVVLKPLVIKGNILI 230
            + +K++          I+L GDS+GGNIA NVA R G     SG  VL      GNIL+
Sbjct: 175 WLKSKKD------SKVHIFLAGDSSGGNIAHNVALRAG----ESGIDVL------GNILL 234

Query: 231 QPFFSGESRTKSEKFLVQPPRSPLTMAVSDTYWRLALPSGANRDHPWCNPSAKGLFKVED 290
            P F G  RT+SEK L    +  +T+   D YW+  LP G +R+HP CNP +     +E 
Sbjct: 235 NPMFGGNERTESEKSL--DGKYFVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLEG 294

Query: 291 LRVLPSLVCISEMDILKDRNLEFCSALHREGKLINYVVYEGVGHAFQVL 324
           +    SLV ++ +D+++D  L +   L + G+ +  +  E     F +L
Sbjct: 295 VSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLL 324

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893358.15.5e-19795.09probable carboxylesterase 6 [Benincasa hispida] >XP_038893359.1 probable carboxy... [more]
XP_022944859.11.6e-19393.93probable carboxylesterase 6 [Cucurbita moschata] >KAG7013040.1 putative carboxyl... [more]
KAG6573982.13.7e-19393.64putative carboxylesterase 6, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_004135407.11.4e-19293.06probable carboxylesterase 6 [Cucumis sativus] >XP_011655765.1 probable carboxyle... [more]
XP_022968602.11.8e-19293.35probable carboxylesterase 6 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SX252.5e-9651.81Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
Q9LFR72.4e-8648.66Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1[more]
Q9FG131.3e-5234.45Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q0ZPV71.5e-4336.79Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1[more]
Q5NUF42.3e-4133.742-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FZC18.0e-19493.93probable carboxylesterase 6 OS=Cucurbita moschata OX=3662 GN=LOC111449259 PE=4 S... [more]
A0A0A0KUG56.7e-19393.06Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6085... [more]
A0A6J1HTY68.8e-19393.35probable carboxylesterase 6 OS=Cucurbita maxima OX=3661 GN=LOC111467774 PE=4 SV=... [more]
A0A1S3BFC87.5e-19292.20probable carboxylesterase 6 OS=Cucumis melo OX=3656 GN=LOC103489265 PE=4 SV=1[more]
A0A5A7SV767.5e-19292.20Putative carboxylesterase 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
Match NameE-valueIdentityDescription
AT1G68620.11.8e-9751.81alpha/beta-Hydrolases superfamily protein [more]
AT5G16080.11.7e-8748.66carboxyesterase 17 [more]
AT5G06570.19.4e-5434.45alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.29.4e-5434.45alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.19.1e-4133.91alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 32..346
e-value: 3.9E-72
score: 245.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 24..344
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 90..321
e-value: 4.7E-55
score: 186.7
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 15..344
NoneNo IPR availablePANTHERPTHR23024:SF409CARBOXYLESTERASE 6-RELATEDcoord: 15..344

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040271.1Lag0040271.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity