Lag0040218 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0040218 vs. NCBI nr
Match: KAG6574020.1 (Chaperonin 60 subunit beta 4, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1061.6 bits (2744), Expect = 2.9e-306
Identity = 556/582 (95.53%), Postives = 569/582 (97.77%), Query Frame = 0

Query: 30  MASSPTPISPLSFPNLKSIPKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKLQVGV 89
           MASSPTPISPLSFPN +S PKPNK P SLPP W PSPKSMPKE+YFNRDGSALKKLQVGV
Sbjct: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60

Query: 90  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 149
           DLVAELVGVTLGPKGRNVVLQ+KYGPPKIVNDGETVLKEIELED LENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120

Query: 150 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSREVEN 209
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGM+PVQIARGIEKTA ALVSELKLMSREVE+
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180

Query: 210 DEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDRGYLS 269
            EIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFD GYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDSGYLS 240

Query: 270 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQEALAP 329
           PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQ ALAP
Sbjct: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300

Query: 330 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 389
           VIRNKLRGVLKAAA+KAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 360

Query: 390 KVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAILQV 449
           KVVISKDSTLIVTDG+TREAVQKRVLQIQKL+ENTEEKFPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVISKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420

Query: 450 GAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQMIG 509
           GAQTQVELKD+QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLEN+EQMIG
Sbjct: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480

Query: 510 AEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 569
           AEIFKRAL YPT+LIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK
Sbjct: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540

Query: 570 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIPRRTPMPSSG 612
           VVRCCLEHAASVAKTFLTSDAVVVDIKEPEP+PRRTP+P SG
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPVPRRTPLPISG 582

BLAST of Lag0040218 vs. NCBI nr
Match: XP_023541180.1 (chaperonin 60 subunit beta 4, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1057.0 bits (2732), Expect = 7.1e-305
Identity = 553/582 (95.02%), Postives = 567/582 (97.42%), Query Frame = 0

Query: 30  MASSPTPISPLSFPNLKSIPKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKLQVGV 89
           MASSPTPISPLSFPN +S PKPNK P SLPP W PSPKSMPKE+YFNRDGSALKKLQVGV
Sbjct: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60

Query: 90  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 149
           DLVAELVGVTLGPKGRNVVLQ+KYGPPKIVNDGETVLKEIELED LENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120

Query: 150 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSREVEN 209
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGM+PVQIARGIEKTA ALVSELKLMSREVE+
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180

Query: 210 DEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDRGYLS 269
            EIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKS+DNSLQIVEGMQFD GYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLS 240

Query: 270 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQEALAP 329
           PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQ ALAP
Sbjct: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300

Query: 330 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 389
           VIRNKLRGVLKAAA+KAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTG EVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSAT 360

Query: 390 KVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAILQV 449
           KVVI+KDSTLIVTDG+TREAVQKRVLQIQKL+ENTEEKFPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420

Query: 450 GAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQMIG 509
           GAQTQVELKD+QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLEN+EQMIG
Sbjct: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480

Query: 510 AEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 569
           AEIFKRAL YPT+LIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK
Sbjct: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540

Query: 570 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIPRRTPMPSSG 612
           VVRCCLEHAASVAKTFLTSDAVVVDIKEPEP PRRTP+P SG
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISG 582

BLAST of Lag0040218 vs. NCBI nr
Match: XP_022968509.1 (chaperonin 60 subunit beta 4, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1056.6 bits (2731), Expect = 9.3e-305
Identity = 554/582 (95.19%), Postives = 568/582 (97.59%), Query Frame = 0

Query: 30  MASSPTPISPLSFPNLKSIPKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKLQVGV 89
           MASSPTPISPLSFPN +S PKPNK P SLPPAW PSPKSMPKE+YFNRDGSALKKLQVGV
Sbjct: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPAWKPSPKSMPKEIYFNRDGSALKKLQVGV 60

Query: 90  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 149
           DLVAELVGVTLGPKGRNVVLQ+KYGPPKIVNDGETVLKEIELED LENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120

Query: 150 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSREVEN 209
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGM+PVQIARGIEKTA ALVSEL+LMSREVE+
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELELMSREVED 180

Query: 210 DEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDRGYLS 269
            EIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFD GYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDSGYLS 240

Query: 270 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQEALAP 329
           PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEG EQ ALAP
Sbjct: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGFEQAALAP 300

Query: 330 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 389
           VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 360

Query: 390 KVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAILQV 449
           KVVI+KDSTLIVTDG+TREAVQKRVLQIQKL+ENTEEKFPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420

Query: 450 GAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQMIG 509
           GAQTQVELKD+QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLEN+EQMIG
Sbjct: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480

Query: 510 AEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 569
           AEIFKRAL YPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK
Sbjct: 481 AEIFKRALSYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540

Query: 570 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIPRRTPMPSSG 612
           VVRCCLEHAASVAKTFLTSDAVVVD+KEPEP PRRT +P+SG
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVDMKEPEPFPRRTTLPNSG 582

BLAST of Lag0040218 vs. NCBI nr
Match: XP_022945344.1 (chaperonin 60 subunit beta 4, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1054.3 bits (2725), Expect = 4.6e-304
Identity = 553/582 (95.02%), Postives = 566/582 (97.25%), Query Frame = 0

Query: 30  MASSPTPISPLSFPNLKSIPKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKLQVGV 89
           MASSPTPISPLSFPN +S PKPNK P SLPP W PSPKSMPKE+YFNRDGSALKKLQVGV
Sbjct: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60

Query: 90  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 149
           DLVAELVGVTLGPKGRNVVLQ KYGPPKIVNDGETVLKEIELED LENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQRKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120

Query: 150 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSREVEN 209
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGM+PVQIARGIEKTA ALVSELKLMSREVE+
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180

Query: 210 DEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDRGYLS 269
            EIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFD GYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDSGYLS 240

Query: 270 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQEALAP 329
           PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQ ALAP
Sbjct: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300

Query: 330 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 389
           VIRNKLRGVLKAAA+KAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 360

Query: 390 KVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAILQV 449
           KVVI+KDSTLIVTDG+TREAVQKRVLQIQKL+ENTEEKFPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420

Query: 450 GAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQMIG 509
           GAQTQVELKD+QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLEN+EQMIG
Sbjct: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480

Query: 510 AEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 569
           AEIFKRAL YPT+LIARNAGVNGSVVIDKVLINNDMN+GYNAATDHYEDLMKAGIMDPSK
Sbjct: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNFGYNAATDHYEDLMKAGIMDPSK 540

Query: 570 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIPRRTPMPSSG 612
           VVRCCLEHAASVAKTFLTSDAVVVDIKEPE  PRRTP+P SG
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPESFPRRTPLPISG 582

BLAST of Lag0040218 vs. NCBI nr
Match: XP_038892006.1 (ruBisCO large subunit-binding protein subunit beta, chloroplastic [Benincasa hispida])

HSP 1 Score: 1044.3 bits (2699), Expect = 4.8e-301
Identity = 549/591 (92.89%), Postives = 568/591 (96.11%), Query Frame = 0

Query: 30  MASSPTPISPLSFPN------LKSIPKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALK 89
           MASSPTPI PLSFPN       K  P PN+ PSSLPPA NP+PKS PKELYFNRDGS  K
Sbjct: 1   MASSPTPIPPLSFPNPTSRSRTKPRPIPNRPPSSLPPALNPTPKSPPKELYFNRDGSTFK 60

Query: 90  KLQVGVDLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLV 149
           KLQVGV+LVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLV
Sbjct: 61  KLQVGVNLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLV 120

Query: 150 RQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLM 209
           RQAGAKTNDLAGDGSTTSV+LAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKL+
Sbjct: 121 RQAGAKTNDLAGDGSTTSVILAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLL 180

Query: 210 SREVENDEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQF 269
           SREVE+ EIAHVAAVSAGNDYAVGN++S ALRQVGRKGVVQIEKGKSIDNSLQIVEGMQF
Sbjct: 181 SREVEDHEIAHVAAVSAGNDYAVGNLVSHALRQVGRKGVVQIEKGKSIDNSLQIVEGMQF 240

Query: 270 DRGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIE 329
           DRGYLSPYFVTDR+KM+VEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIE
Sbjct: 241 DRGYLSPYFVTDRKKMVVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIE 300

Query: 330 QEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKE 389
           QEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGA V+REDSGLTLEKTGKE
Sbjct: 301 QEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGAIVVREDSGLTLEKTGKE 360

Query: 390 VLGSATKVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGR 449
           VLGSA+KVVISKDSTLIVTDG+TREAV+KRVLQIQKLMENTEEKFPKKILNERIARLSGR
Sbjct: 361 VLGSASKVVISKDSTLIVTDGNTREAVRKRVLQIQKLMENTEEKFPKKILNERIARLSGR 420

Query: 450 IAILQVGAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLEN 509
           IAILQVGAQT+VELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLEN
Sbjct: 421 IAILQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLEN 480

Query: 510 DEQMIGAEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAG 569
           DEQMIGAEIFKRAL YPTKLIARNAGVNGSVVIDKVLINND++YGYNAATD YEDLMKAG
Sbjct: 481 DEQMIGAEIFKRALSYPTKLIARNAGVNGSVVIDKVLINNDLDYGYNAATDRYEDLMKAG 540

Query: 570 IMDPSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIPRRTPMPSSGKWV 615
           IMDPSKVVRCCLEHAASVAKTFLTSDAVVVD+KEPEPI +RTPMP SGKWV
Sbjct: 541 IMDPSKVVRCCLEHAASVAKTFLTSDAVVVDMKEPEPISKRTPMPISGKWV 591

BLAST of Lag0040218 vs. ExPASy Swiss-Prot
Match: Q9C667 (Chaperonin 60 subunit beta 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN60B4 PE=1 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 3.9e-229
Identity = 417/582 (71.65%), Postives = 496/582 (85.22%), Query Frame = 0

Query: 30  MASSPTPISPLSFPNLKSIPKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKLQVGV 89
           MA S   +S L   +     KP+   SS  P +    ++  KE++FNRDGS  KKLQ G 
Sbjct: 1   MAFSQAALSALPLSDRTFRKKPSS-SSSSSPNFVLRVRAAAKEVHFNRDGSVTKKLQAGA 60

Query: 90  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 149
           D+VA+L+GVTLGPKGRNVVLQNKYGPP+IVNDGETVLKEIELEDPLENVGVKLVRQAGAK
Sbjct: 61  DMVAKLLGVTLGPKGRNVVLQNKYGPPRIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 120

Query: 150 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSREVEN 209
           TNDLAGDGSTTS++LA GLI EG+KVIS+G NP+Q+ARGIEKT KALV ELK MSRE+E+
Sbjct: 121 TNDLAGDGSTTSIILAHGLITEGIKVISAGTNPIQVARGIEKTTKALVLELKSMSREIED 180

Query: 210 DEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDRGYLS 269
            E+AHVAAVSAGNDY VGNMIS+A +QVGR GVV IEKGK + N+L+IVEGMQF+RGYLS
Sbjct: 181 HELAHVAAVSAGNDYEVGNMISNAFQQVGRTGVVTIEKGKYLVNNLEIVEGMQFNRGYLS 240

Query: 270 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQEALAP 329
           PYFVTDR+K   EFHDCKLLLVDKKI++PK+MFKILDSAVKE++P++I+AE IEQ+ALAP
Sbjct: 241 PYFVTDRRKREAEFHDCKLLLVDKKITNPKDMFKILDSAVKEEFPVLIVAEDIEQDALAP 300

Query: 330 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 389
           VIRNKL+G LK AAIKAPAFGERKSH LDD+A  TGATVIR++ GL+LEK GKEVLG+A 
Sbjct: 301 VIRNKLKGNLKVAAIKAPAFGERKSHCLDDLAIFTGATVIRDEMGLSLEKAGKEVLGTAK 360

Query: 390 KVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAILQV 449
           +V+++KDSTLIVT+G T++AV +RV QI+ L+ENTEE F KKILNER+ARLSG IAI+QV
Sbjct: 361 RVLVTKDSTLIVTNGFTQKAVDERVSQIKNLIENTEENFQKKILNERVARLSGGIAIIQV 420

Query: 450 GAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQMIG 509
           GA TQVELKD+QL++EDALNA+K+AIEEG+VVGGGC LLRL+TKVD IK  L+N EQ IG
Sbjct: 421 GALTQVELKDKQLKVEDALNATKSAIEEGIVVGGGCALLRLATKVDRIKETLDNTEQKIG 480

Query: 510 AEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 569
           AEIFK+AL YP +LIA+NA  NG++VI+KVL N +  YGYNAA + YEDLM AGI+DP+K
Sbjct: 481 AEIFKKALSYPIRLIAKNADTNGNIVIEKVLSNKNTMYGYNAAKNQYEDLMLAGIIDPTK 540

Query: 570 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIP-RRTPMPSS 611
           VVRCCLEHA+SVA+TFLTSD VVV+IKE +P P    P+P+S
Sbjct: 541 VVRCCLEHASSVAQTFLTSDCVVVEIKEIKPRPIINPPLPTS 581

BLAST of Lag0040218 vs. ExPASy Swiss-Prot
Match: P08927 (RuBisCO large subunit-binding protein subunit beta, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=2)

HSP 1 Score: 747.7 bits (1929), Expect = 1.2e-214
Identity = 381/549 (69.40%), Postives = 470/549 (85.61%), Query Frame = 0

Query: 63  NPSPKSMPKELYFNRDGSALKKLQVGVDLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDG 122
           N    +M KEL+FN+DGSA+KKLQ GV+ +A+LVGVTLGPKGRNVVL++KYG PKIVNDG
Sbjct: 43  NVKVSAMAKELHFNKDGSAIKKLQNGVNKLADLVGVTLGPKGRNVVLESKYGSPKIVNDG 102

Query: 123 ETVLKEIELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNP 182
            TV KE+ELEDP+EN+G KLVRQA AKTNDLAGDG+TTSVVLAQGLIAEG+KV+++G NP
Sbjct: 103 VTVAKEVELEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANP 162

Query: 183 VQIARGIEKTAKALVSELKLMSREVENDEIAHVAAVSAGNDYAVGNMISDALRQVGRKGV 242
           V I RGIEKT+KALV+ELK MS+EVE+ E+A VAAVSAGN++ VGNMI++AL +VGRKGV
Sbjct: 163 VLITRGIEKTSKALVAELKKMSKEVEDSELADVAAVSAGNNHEVGNMIAEALSKVGRKGV 222

Query: 243 VQIEKGKSIDNSLQIVEGMQFDRGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMF 302
           V +E+GKS +NSL +VEGMQFDRGY+SPYFVTD +KM VEF +CKLLLVDKKI++ +++ 
Sbjct: 223 VTLEEGKSAENSLYVVEGMQFDRGYISPYFVTDSEKMTVEFENCKLLLVDKKITNARDLI 282

Query: 303 KILDSAVKEKYPIVILAEGIEQEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIAT 362
            IL+ A++  +PIVI+AE IEQEALA ++ NKLRG LK AA+KAP FGERKS YLDDIA 
Sbjct: 283 NILEDAIRSGFPIVIIAEDIEQEALATLVVNKLRGSLKIAALKAPGFGERKSQYLDDIAI 342

Query: 363 LTGATVIREDSGLTLEKTGKEVLGSATKVVISKDSTLIVTDGSTREAVQKRVLQIQKLME 422
           LTG TVIRE+ GLTL+K  KEVLG+A KVV++KD+T IV DGST+EAV KRV QI+  +E
Sbjct: 343 LTGGTVIREEVGLTLDKADKEVLGNAAKVVLTKDTTTIVGDGSTQEAVNKRVSQIKNQIE 402

Query: 423 NTEEKFPKKILNERIARLSGRIAILQVGAQTQVELKDRQLRIEDALNASKAAIEEGVVVG 482
             E+++ K+ L+ERIA+LSG +A++QVGAQT+ ELK+++LR+EDALNA+KAA+EEG+VVG
Sbjct: 403 AAEQEYEKEKLSERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVG 462

Query: 483 GGCCLLRLSTKVDAIKNVLENDEQMIGAEIFKRALCYPTKLIARNAGVNGSVVIDKVLIN 542
           GGC LLRL++KVDAIK+ L NDE+ +GA+I KRAL YP KLIA+NAGVNGSVV +KVL +
Sbjct: 463 GGCTLLRLASKVDAIKDTLANDEEKVGADIVKRALSYPLKLIAKNAGVNGSVVSEKVLSS 522

Query: 543 NDMNYGYNAATDHYEDLMKAGIMDPSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIP 602
           ++  YGYNAAT  YEDLM AGI+DP+KVVRCCLEHA+SVAKTFL SD VVV+IKEPE  P
Sbjct: 523 DNPKYGYNAATGKYEDLMAAGIIDPTKVVRCCLEHASSVAKTFLMSDCVVVEIKEPESAP 582

Query: 603 RRTPMPSSG 612
              PM +SG
Sbjct: 583 VGNPMDNSG 591

BLAST of Lag0040218 vs. ExPASy Swiss-Prot
Match: Q9LJE4 (Chaperonin 60 subunit beta 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN60B2 PE=1 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 2.1e-214
Identity = 383/586 (65.36%), Postives = 486/586 (82.94%), Query Frame = 0

Query: 26  PAGTMASSPTPISPLSFPNLKSIPKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKL 85
           P     SS T IS  SF    ++      P+ +  A         KEL+FN+DG+ ++KL
Sbjct: 17  PNAIKLSSATSISSSSFGRRHNVCVRRSRPAIVCAA---------KELHFNKDGTTIRKL 76

Query: 86  QVGVDLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQ 145
           Q GV+ +A+LVGVTLGPKGRNVVL++KYG P+IVNDG TV +E+ELEDP+EN+G KLVRQ
Sbjct: 77  QTGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQ 136

Query: 146 AGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSR 205
           A AKTNDLAGDG+TTSVVLAQG IAEG+KV+++G NPV I RGIEKTAKALV+ELKLMS+
Sbjct: 137 AAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVNELKLMSK 196

Query: 206 EVENDEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDR 265
           EVE+ E+A VAAVSAGN++ VG+MI++A+ +VGRKGVV +E+GKS +N+L +VEGMQFDR
Sbjct: 197 EVEDSELADVAAVSAGNNHEVGSMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDR 256

Query: 266 GYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQE 325
           GY+SPYFVTD +KM VE+ +CKLLLVDKK+++ +++  +L+ A++  YPI+I+AE IEQE
Sbjct: 257 GYISPYFVTDSEKMSVEYDNCKLLLVDKKVTNARDLVGVLEDAIRGGYPILIIAEDIEQE 316

Query: 326 ALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVL 385
           ALA ++ NKLRG LK AA+KAP FGERKS YLDDIA LTGATVIRE+ GL+L+K GKEVL
Sbjct: 317 ALATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVL 376

Query: 386 GSATKVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIA 445
           G+A+KVV++K+ T IV DG+T+EAV KRV+QI+ L+E  E+ + K+ LNERIA+LSG +A
Sbjct: 377 GNASKVVLTKEMTTIVGDGTTQEAVNKRVVQIRNLIEQAEQDYEKEKLNERIAKLSGGVA 436

Query: 446 ILQVGAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDE 505
           ++QVGAQT+ ELK+++LR+EDALNA+KAA+EEG+VVGGGC LLRL++KVDAIK+ LENDE
Sbjct: 437 VIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKDTLENDE 496

Query: 506 QMIGAEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIM 565
           + +GAEI KRAL YP KLIA+NAGVNGSVV +KVL N+++ +GYNAAT  YEDLM AGI+
Sbjct: 497 EKVGAEIVKRALSYPLKLIAKNAGVNGSVVSEKVLANDNVKFGYNAATGKYEDLMAAGII 556

Query: 566 DPSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIPRRTPMPSSG 612
           DP+KVVRCCLEHAASVAKTFL SD VVV+I EPEP+P   PM +SG
Sbjct: 557 DPTKVVRCCLEHAASVAKTFLMSDCVVVEIPEPEPVPAGNPMDNSG 593

BLAST of Lag0040218 vs. ExPASy Swiss-Prot
Match: P21240 (Chaperonin 60 subunit beta 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN60B1 PE=1 SV=3)

HSP 1 Score: 741.9 bits (1914), Expect = 6.7e-213
Identity = 378/576 (65.62%), Postives = 486/576 (84.38%), Query Frame = 0

Query: 37  ISPLSFPNLKSI-PKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKLQVGVDLVAEL 96
           +S  SF   +S+ P+P +  S++  A         KEL+FN+DG+ +++LQ GV+ +A+L
Sbjct: 30  LSSSSFGRRQSVCPRPRRSSSAIVCA--------AKELHFNKDGTTIRRLQAGVNKLADL 89

Query: 97  VGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAG 156
           VGVTLGPKGRNVVL++KYG P+IVNDG TV +E+ELEDP+EN+G KLVRQA AKTNDLAG
Sbjct: 90  VGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTNDLAG 149

Query: 157 DGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSREVENDEIAHV 216
           DG+TTSVVLAQG IAEG+KV+++G NPV I RGIEKTAKALV+ELK MS+EVE+ E+A V
Sbjct: 150 DGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVTELKKMSKEVEDSELADV 209

Query: 217 AAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDRGYLSPYFVTD 276
           AAVSAGN+  +GNMI++A+ +VGRKGVV +E+GKS +N+L +VEGMQFDRGY+SPYFVTD
Sbjct: 210 AAVSAGNNDEIGNMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDRGYISPYFVTD 269

Query: 277 RQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQEALAPVIRNKL 336
            +KM VEF +CKLLLVDKKI++ +++  +L+ A++  YPI+I+AE IEQEALA ++ NKL
Sbjct: 270 SEKMSVEFDNCKLLLVDKKITNARDLVGVLEDAIRGGYPILIIAEDIEQEALATLVVNKL 329

Query: 337 RGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSATKVVISK 396
           RG LK AA++AP FGERKS YLDDIA LTGATVIRE+ GL+L+K GKEVLG+A+KVV++K
Sbjct: 330 RGTLKIAALRAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLGNASKVVLTK 389

Query: 397 DSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAILQVGAQTQV 456
           +++ IV DGST++AV+KRV QI+ L+E  E+ + K+ LNERIA+LSG +A++QVGAQT+ 
Sbjct: 390 ETSTIVGDGSTQDAVKKRVTQIKNLIEQAEQDYEKEKLNERIAKLSGGVAVIQVGAQTET 449

Query: 457 ELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQMIGAEIFKR 516
           ELK+++LR+EDALNA+KAA+EEG+VVGGGC LLRL++KVDAIK  L+NDE+ +GA+I KR
Sbjct: 450 ELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKATLDNDEEKVGADIVKR 509

Query: 517 ALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSKVVRCCL 576
           AL YP KLIA+NAGVNGSVV +KVL N+++ +GYNAAT  YEDLM AGI+DP+KVVRCCL
Sbjct: 510 ALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFGYNAATGKYEDLMAAGIIDPTKVVRCCL 569

Query: 577 EHAASVAKTFLTSDAVVVDIKEPEPIPRRTPMPSSG 612
           EHAASVAKTFL SD VVV+IKEPEP+P   PM +SG
Sbjct: 570 EHAASVAKTFLMSDCVVVEIKEPEPVPVGNPMDNSG 597

BLAST of Lag0040218 vs. ExPASy Swiss-Prot
Match: P21241 (RuBisCO large subunit-binding protein subunit beta, chloroplastic OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 1.6e-211
Identity = 369/540 (68.33%), Postives = 467/540 (86.48%), Query Frame = 0

Query: 63  NPSPKSMPKELYFNRDGSALKKLQVGVDLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDG 122
           +P+     KEL+FN+DG+ ++KLQ GV+ +A+LVGVTLGPKGRNVVL++KYG P+IVNDG
Sbjct: 49  SPAVVCAAKELHFNKDGTTIRKLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDG 108

Query: 123 ETVLKEIELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNP 182
            TV +E+ELEDP+EN+G KLVRQA AKTNDLAGDG+TTSVVLAQG IAEG+KV+++G NP
Sbjct: 109 VTVAREVELEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANP 168

Query: 183 VQIARGIEKTAKALVSELKLMSREVENDEIAHVAAVSAGNDYAVGNMISDALRQVGRKGV 242
           V I RGIEKTAKALV+ELK MS+EVE+ E+A VAAVSAGN+  +G+MI++A+ +VGRKGV
Sbjct: 169 VLITRGIEKTAKALVAELKKMSKEVEDSELADVAAVSAGNNAEIGSMIAEAMSRVGRKGV 228

Query: 243 VQIEKGKSIDNSLQIVEGMQFDRGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMF 302
           V +E+GKS +N+L +VEGMQFDRGY+SPYFVTD +KM VEF +CKLLLVDKKI++ +++ 
Sbjct: 229 VTLEEGKSAENALYVVEGMQFDRGYVSPYFVTDSEKMSVEFDNCKLLLVDKKITNARDLV 288

Query: 303 KILDSAVKEKYPIVILAEGIEQEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIAT 362
            +L+ A++  YPI+I+AE IEQEALA ++ NKLRG LK AA+KAP FGERKS YLDDIA 
Sbjct: 289 GVLEDAIRGGYPILIIAEDIEQEALATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIAI 348

Query: 363 LTGATVIREDSGLTLEKTGKEVLGSATKVVISKDSTLIVTDGSTREAVQKRVLQIQKLME 422
           LTGATVIRE+ GL+L+K GKEVLG A KVV++K+++ IV DGST++AVQKRV QI+ L+E
Sbjct: 349 LTGATVIREEVGLSLDKAGKEVLGHAAKVVLTKETSTIVGDGSTQDAVQKRVTQIKNLIE 408

Query: 423 NTEEKFPKKILNERIARLSGRIAILQVGAQTQVELKDRQLRIEDALNASKAAIEEGVVVG 482
             E+ + K+ LNERIA+LSG +A++QVGAQT+ ELK+++LR+EDALNA+KAA+EEG+VVG
Sbjct: 409 QAEQDYEKEKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVG 468

Query: 483 GGCCLLRLSTKVDAIKNVLENDEQMIGAEIFKRALCYPTKLIARNAGVNGSVVIDKVLIN 542
           GGC LLRL++KVDAIK  L+NDE+ +GA+I KRAL YP KLIA+NAGVNGSVV +KVL N
Sbjct: 469 GGCTLLRLASKVDAIKAALDNDEEKVGADIVKRALSYPLKLIAKNAGVNGSVVSEKVLSN 528

Query: 543 NDMNYGYNAATDHYEDLMKAGIMDPSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIP 602
           +++ +GYNAAT  YEDLM AGI+DP+KVVRCCLEHAASVAKTFL SD VVV+IKEPEP+P
Sbjct: 529 DNVKFGYNAATGKYEDLMAAGIIDPTKVVRCCLEHAASVAKTFLMSDCVVVEIKEPEPVP 588

BLAST of Lag0040218 vs. ExPASy TrEMBL
Match: A0A6J1HZU9 (chaperonin 60 subunit beta 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111467722 PE=3 SV=1)

HSP 1 Score: 1056.6 bits (2731), Expect = 4.5e-305
Identity = 554/582 (95.19%), Postives = 568/582 (97.59%), Query Frame = 0

Query: 30  MASSPTPISPLSFPNLKSIPKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKLQVGV 89
           MASSPTPISPLSFPN +S PKPNK P SLPPAW PSPKSMPKE+YFNRDGSALKKLQVGV
Sbjct: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPAWKPSPKSMPKEIYFNRDGSALKKLQVGV 60

Query: 90  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 149
           DLVAELVGVTLGPKGRNVVLQ+KYGPPKIVNDGETVLKEIELED LENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120

Query: 150 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSREVEN 209
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGM+PVQIARGIEKTA ALVSEL+LMSREVE+
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELELMSREVED 180

Query: 210 DEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDRGYLS 269
            EIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFD GYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDSGYLS 240

Query: 270 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQEALAP 329
           PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEG EQ ALAP
Sbjct: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGFEQAALAP 300

Query: 330 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 389
           VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 360

Query: 390 KVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAILQV 449
           KVVI+KDSTLIVTDG+TREAVQKRVLQIQKL+ENTEEKFPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420

Query: 450 GAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQMIG 509
           GAQTQVELKD+QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLEN+EQMIG
Sbjct: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480

Query: 510 AEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 569
           AEIFKRAL YPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK
Sbjct: 481 AEIFKRALSYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540

Query: 570 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIPRRTPMPSSG 612
           VVRCCLEHAASVAKTFLTSDAVVVD+KEPEP PRRT +P+SG
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVDMKEPEPFPRRTTLPNSG 582

BLAST of Lag0040218 vs. ExPASy TrEMBL
Match: A0A6J1G0R7 (chaperonin 60 subunit beta 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111449606 PE=3 SV=1)

HSP 1 Score: 1054.3 bits (2725), Expect = 2.2e-304
Identity = 553/582 (95.02%), Postives = 566/582 (97.25%), Query Frame = 0

Query: 30  MASSPTPISPLSFPNLKSIPKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKLQVGV 89
           MASSPTPISPLSFPN +S PKPNK P SLPP W PSPKSMPKE+YFNRDGSALKKLQVGV
Sbjct: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60

Query: 90  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 149
           DLVAELVGVTLGPKGRNVVLQ KYGPPKIVNDGETVLKEIELED LENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQRKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120

Query: 150 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSREVEN 209
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGM+PVQIARGIEKTA ALVSELKLMSREVE+
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180

Query: 210 DEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDRGYLS 269
            EIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFD GYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDSGYLS 240

Query: 270 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQEALAP 329
           PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQ ALAP
Sbjct: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300

Query: 330 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 389
           VIRNKLRGVLKAAA+KAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 360

Query: 390 KVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAILQV 449
           KVVI+KDSTLIVTDG+TREAVQKRVLQIQKL+ENTEEKFPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420

Query: 450 GAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQMIG 509
           GAQTQVELKD+QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLEN+EQMIG
Sbjct: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480

Query: 510 AEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 569
           AEIFKRAL YPT+LIARNAGVNGSVVIDKVLINNDMN+GYNAATDHYEDLMKAGIMDPSK
Sbjct: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNFGYNAATDHYEDLMKAGIMDPSK 540

Query: 570 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIPRRTPMPSSG 612
           VVRCCLEHAASVAKTFLTSDAVVVDIKEPE  PRRTP+P SG
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPESFPRRTPLPISG 582

BLAST of Lag0040218 vs. ExPASy TrEMBL
Match: A0A6J1D5S9 (chaperonin 60 subunit beta 4, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111017567 PE=3 SV=1)

HSP 1 Score: 1025.0 bits (2649), Expect = 1.5e-295
Identity = 540/578 (93.43%), Postives = 559/578 (96.71%), Query Frame = 0

Query: 30  MASSPTPISPLSFPNLKSIPKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKLQVGV 89
           M SSPTPISPLS PN  S PK N+ PSSLPPAWNPSPKS+PKELYFNRDGSALKKLQVGV
Sbjct: 1   MTSSPTPISPLSLPN--SRPKSNRPPSSLPPAWNPSPKSVPKELYFNRDGSALKKLQVGV 60

Query: 90  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 149
           DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 120

Query: 150 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSREVEN 209
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIE TA+ALVSELKL+SREVE+
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEMTARALVSELKLISREVED 180

Query: 210 DEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDRGYLS 269
            EIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKS+DNSLQIVEGMQFDRGYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDRGYLS 240

Query: 270 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQEALAP 329
           PYFVTDR+KM+ EFHDCKLLL+DKKISDPKEMFKILDSAVKEK+PIVILAEGIEQEALAP
Sbjct: 241 PYFVTDRRKMMAEFHDCKLLLIDKKISDPKEMFKILDSAVKEKFPIVILAEGIEQEALAP 300

Query: 330 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 389
           VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 360

Query: 390 KVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAILQV 449
           KVVISKDSTLIVTDG TREAVQKRVLQIQKLMENTEE FPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVISKDSTLIVTDGRTREAVQKRVLQIQKLMENTEENFPKKILNERIARLSGRIAILQV 420

Query: 450 GAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQMIG 509
           GAQT+VELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVD IK+VLEN+EQMIG
Sbjct: 421 GAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDDIKSVLENEEQMIG 480

Query: 510 AEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 569
           AEIF+RAL YPTKLIARNAGVNG++VIDKVLIN D+ YGYNAATD YEDLMKAGIMDPSK
Sbjct: 481 AEIFRRALSYPTKLIARNAGVNGNMVIDKVLINKDIKYGYNAATDCYEDLMKAGIMDPSK 540

Query: 570 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIPRRTPM 608
           VVRCCLEHAASVAKTFLTSDAVVV+IKEP+PIPRR  M
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVEIKEPKPIPRRRRM 576

BLAST of Lag0040218 vs. ExPASy TrEMBL
Match: A0A6J1D776 (chaperonin 60 subunit beta 4, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017567 PE=3 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 6.1e-294
Identity = 540/581 (92.94%), Postives = 559/581 (96.21%), Query Frame = 0

Query: 30  MASSPTPISPLSFPNLKSIPKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKLQVGV 89
           M SSPTPISPLS PN  S PK N+ PSSLPPAWNPSPKS+PKELYFNRDGSALKKLQVGV
Sbjct: 1   MTSSPTPISPLSLPN--SRPKSNRPPSSLPPAWNPSPKSVPKELYFNRDGSALKKLQVGV 60

Query: 90  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 149
           DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 120

Query: 150 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSRE--- 209
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIE TA+ALVSELKL+SRE   
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEMTARALVSELKLISREVIK 180

Query: 210 VENDEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDRG 269
           VE+ EIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKS+DNSLQIVEGMQFDRG
Sbjct: 181 VEDHEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDRG 240

Query: 270 YLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQEA 329
           YLSPYFVTDR+KM+ EFHDCKLLL+DKKISDPKEMFKILDSAVKEK+PIVILAEGIEQEA
Sbjct: 241 YLSPYFVTDRRKMMAEFHDCKLLLIDKKISDPKEMFKILDSAVKEKFPIVILAEGIEQEA 300

Query: 330 LAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLG 389
           LAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLG
Sbjct: 301 LAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLG 360

Query: 390 SATKVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAI 449
           SATKVVISKDSTLIVTDG TREAVQKRVLQIQKLMENTEE FPKKILNERIARLSGRIAI
Sbjct: 361 SATKVVISKDSTLIVTDGRTREAVQKRVLQIQKLMENTEENFPKKILNERIARLSGRIAI 420

Query: 450 LQVGAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQ 509
           LQVGAQT+VELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVD IK+VLEN+EQ
Sbjct: 421 LQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDDIKSVLENEEQ 480

Query: 510 MIGAEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMD 569
           MIGAEIF+RAL YPTKLIARNAGVNG++VIDKVLIN D+ YGYNAATD YEDLMKAGIMD
Sbjct: 481 MIGAEIFRRALSYPTKLIARNAGVNGNMVIDKVLINKDIKYGYNAATDCYEDLMKAGIMD 540

Query: 570 PSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIPRRTPM 608
           PSKVVRCCLEHAASVAKTFLTSDAVVV+IKEP+PIPRR  M
Sbjct: 541 PSKVVRCCLEHAASVAKTFLTSDAVVVEIKEPKPIPRRRRM 579

BLAST of Lag0040218 vs. ExPASy TrEMBL
Match: A0A5D3CCJ6 (RuBisCO large subunit-binding protein subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00380 PE=3 SV=1)

HSP 1 Score: 1016.9 bits (2628), Expect = 4.0e-293
Identity = 535/592 (90.37%), Postives = 561/592 (94.76%), Query Frame = 0

Query: 30  MASSPTPISPLSFPNLKS----------IPKPNKLPSSLPPAWNPSPKSMPKELYFNRDG 89
           MASSP+PISPLSF N  S          +P PNK PSS PPA NP+PK  PKELYFN DG
Sbjct: 1   MASSPSPISPLSFTNPISTSRTKPKPLPLPFPNKPPSSFPPASNPTPKPPPKELYFNHDG 60

Query: 90  SALKKLQVGVDLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVG 149
           S  KKLQ+GV+LVAELVG+TLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVG
Sbjct: 61  STFKKLQIGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVG 120

Query: 150 VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSE 209
           VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVI+SGMNPVQIARGIEKTAKALVSE
Sbjct: 121 VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSE 180

Query: 210 LKLMSREVENDEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVE 269
           LKLMSREVE+ EIAHVAAVSAGNDYAVGN++SDALRQVG KGVVQIEKGKS+DNSLQIVE
Sbjct: 181 LKLMSREVEDHEIAHVAAVSAGNDYAVGNLVSDALRQVGWKGVVQIEKGKSVDNSLQIVE 240

Query: 270 GMQFDRGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILA 329
           GMQFDRGYLSPYFVTDR+KM+VEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILA
Sbjct: 241 GMQFDRGYLSPYFVTDRRKMVVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILA 300

Query: 330 EGIEQEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEK 389
           EGIEQEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATV+RED+GLTLEK
Sbjct: 301 EGIEQEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEK 360

Query: 390 TGKEVLGSATKVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIAR 449
           TGKEVLGSA KVVISKDSTLIVTDG+TREAVQKRV+QIQKLMENTEEKFPKKILNERIAR
Sbjct: 361 TGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVIQIQKLMENTEEKFPKKILNERIAR 420

Query: 450 LSGRIAILQVGAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN 509
           LSGRIAI+QVGAQT+VELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN
Sbjct: 421 LSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN 480

Query: 510 VLENDEQMIGAEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDL 569
           VLENDEQMIGAEIFKRAL YPTKLIARNAGVNGSVVIDK+L NND++YGYNAATD YE+L
Sbjct: 481 VLENDEQMIGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYENL 540

Query: 570 MKAGIMDPSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIPRRTPMPSSG 612
           MKAGIMDPSKVVRCCLEHAASV+KTFLTSDAVVVD+KE + IPRRTPMP SG
Sbjct: 541 MKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKESQLIPRRTPMPISG 592

BLAST of Lag0040218 vs. TAIR 10
Match: AT1G26230.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 795.8 bits (2054), Expect = 2.8e-230
Identity = 417/582 (71.65%), Postives = 496/582 (85.22%), Query Frame = 0

Query: 30  MASSPTPISPLSFPNLKSIPKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKLQVGV 89
           MA S   +S L   +     KP+   SS  P +    ++  KE++FNRDGS  KKLQ G 
Sbjct: 1   MAFSQAALSALPLSDRTFRKKPSS-SSSSSPNFVLRVRAAAKEVHFNRDGSVTKKLQAGA 60

Query: 90  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 149
           D+VA+L+GVTLGPKGRNVVLQNKYGPP+IVNDGETVLKEIELEDPLENVGVKLVRQAGAK
Sbjct: 61  DMVAKLLGVTLGPKGRNVVLQNKYGPPRIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 120

Query: 150 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSREVEN 209
           TNDLAGDGSTTS++LA GLI EG+KVIS+G NP+Q+ARGIEKT KALV ELK MSRE+E+
Sbjct: 121 TNDLAGDGSTTSIILAHGLITEGIKVISAGTNPIQVARGIEKTTKALVLELKSMSREIED 180

Query: 210 DEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDRGYLS 269
            E+AHVAAVSAGNDY VGNMIS+A +QVGR GVV IEKGK + N+L+IVEGMQF+RGYLS
Sbjct: 181 HELAHVAAVSAGNDYEVGNMISNAFQQVGRTGVVTIEKGKYLVNNLEIVEGMQFNRGYLS 240

Query: 270 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQEALAP 329
           PYFVTDR+K   EFHDCKLLLVDKKI++PK+MFKILDSAVKE++P++I+AE IEQ+ALAP
Sbjct: 241 PYFVTDRRKREAEFHDCKLLLVDKKITNPKDMFKILDSAVKEEFPVLIVAEDIEQDALAP 300

Query: 330 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 389
           VIRNKL+G LK AAIKAPAFGERKSH LDD+A  TGATVIR++ GL+LEK GKEVLG+A 
Sbjct: 301 VIRNKLKGNLKVAAIKAPAFGERKSHCLDDLAIFTGATVIRDEMGLSLEKAGKEVLGTAK 360

Query: 390 KVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAILQV 449
           +V+++KDSTLIVT+G T++AV +RV QI+ L+ENTEE F KKILNER+ARLSG IAI+QV
Sbjct: 361 RVLVTKDSTLIVTNGFTQKAVDERVSQIKNLIENTEENFQKKILNERVARLSGGIAIIQV 420

Query: 450 GAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQMIG 509
           GA TQVELKD+QL++EDALNA+K+AIEEG+VVGGGC LLRL+TKVD IK  L+N EQ IG
Sbjct: 421 GALTQVELKDKQLKVEDALNATKSAIEEGIVVGGGCALLRLATKVDRIKETLDNTEQKIG 480

Query: 510 AEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 569
           AEIFK+AL YP +LIA+NA  NG++VI+KVL N +  YGYNAA + YEDLM AGI+DP+K
Sbjct: 481 AEIFKKALSYPIRLIAKNADTNGNIVIEKVLSNKNTMYGYNAAKNQYEDLMLAGIIDPTK 540

Query: 570 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIP-RRTPMPSS 611
           VVRCCLEHA+SVA+TFLTSD VVV+IKE +P P    P+P+S
Sbjct: 541 VVRCCLEHASSVAQTFLTSDCVVVEIKEIKPRPIINPPLPTS 581

BLAST of Lag0040218 vs. TAIR 10
Match: AT1G26230.2 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 773.5 bits (1996), Expect = 1.5e-223
Identity = 395/527 (74.95%), Postives = 468/527 (88.80%), Query Frame = 0

Query: 85  LQVGVDLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVR 144
           ++ G D+VA+L+GVTLGPKGRNVVLQNKYGPP+IVNDGETVLKEIELEDPLENVGVKLVR
Sbjct: 3   VKAGADMVAKLLGVTLGPKGRNVVLQNKYGPPRIVNDGETVLKEIELEDPLENVGVKLVR 62

Query: 145 QAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMS 204
           QAGAKTNDLAGDGSTTS++LA GLI EG+KVIS+G NP+Q+ARGIEKT KALV ELK MS
Sbjct: 63  QAGAKTNDLAGDGSTTSIILAHGLITEGIKVISAGTNPIQVARGIEKTTKALVLELKSMS 122

Query: 205 REVENDEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFD 264
           RE+E+ E+AHVAAVSAGNDY VGNMIS+A +QVGR GVV IEKGK + N+L+IVEGMQF+
Sbjct: 123 REIEDHELAHVAAVSAGNDYEVGNMISNAFQQVGRTGVVTIEKGKYLVNNLEIVEGMQFN 182

Query: 265 RGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQ 324
           RGYLSPYFVTDR+K   EFHDCKLLLVDKKI++PK+MFKILDSAVKE++P++I+AE IEQ
Sbjct: 183 RGYLSPYFVTDRRKREAEFHDCKLLLVDKKITNPKDMFKILDSAVKEEFPVLIVAEDIEQ 242

Query: 325 EALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEV 384
           +ALAPVIRNKL+G LK AAIKAPAFGERKSH LDD+A  TGATVIR++ GL+LEK GKEV
Sbjct: 243 DALAPVIRNKLKGNLKVAAIKAPAFGERKSHCLDDLAIFTGATVIRDEMGLSLEKAGKEV 302

Query: 385 LGSATKVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRI 444
           LG+A +V+++KDSTLIVT+G T++AV +RV QI+ L+ENTEE F KKILNER+ARLSG I
Sbjct: 303 LGTAKRVLVTKDSTLIVTNGFTQKAVDERVSQIKNLIENTEENFQKKILNERVARLSGGI 362

Query: 445 AILQVGAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLEND 504
           AI+QVGA TQVELKD+QL++EDALNA+K+AIEEG+VVGGGC LLRL+TKVD IK  L+N 
Sbjct: 363 AIIQVGALTQVELKDKQLKVEDALNATKSAIEEGIVVGGGCALLRLATKVDRIKETLDNT 422

Query: 505 EQMIGAEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGI 564
           EQ IGAEIFK+AL YP +LIA+NA  NG++VI+KVL N +  YGYNAA + YEDLM AGI
Sbjct: 423 EQKIGAEIFKKALSYPIRLIAKNADTNGNIVIEKVLSNKNTMYGYNAAKNQYEDLMLAGI 482

Query: 565 MDPSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIP-RRTPMPSS 611
           +DP+KVVRCCLEHA+SVA+TFLTSD VVV+IKE +P P    P+P+S
Sbjct: 483 IDPTKVVRCCLEHASSVAQTFLTSDCVVVEIKEIKPRPIINPPLPTS 529

BLAST of Lag0040218 vs. TAIR 10
Match: AT3G13470.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 746.9 bits (1927), Expect = 1.5e-215
Identity = 383/586 (65.36%), Postives = 486/586 (82.94%), Query Frame = 0

Query: 26  PAGTMASSPTPISPLSFPNLKSIPKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKL 85
           P     SS T IS  SF    ++      P+ +  A         KEL+FN+DG+ ++KL
Sbjct: 17  PNAIKLSSATSISSSSFGRRHNVCVRRSRPAIVCAA---------KELHFNKDGTTIRKL 76

Query: 86  QVGVDLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQ 145
           Q GV+ +A+LVGVTLGPKGRNVVL++KYG P+IVNDG TV +E+ELEDP+EN+G KLVRQ
Sbjct: 77  QTGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQ 136

Query: 146 AGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSR 205
           A AKTNDLAGDG+TTSVVLAQG IAEG+KV+++G NPV I RGIEKTAKALV+ELKLMS+
Sbjct: 137 AAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVNELKLMSK 196

Query: 206 EVENDEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDR 265
           EVE+ E+A VAAVSAGN++ VG+MI++A+ +VGRKGVV +E+GKS +N+L +VEGMQFDR
Sbjct: 197 EVEDSELADVAAVSAGNNHEVGSMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDR 256

Query: 266 GYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQE 325
           GY+SPYFVTD +KM VE+ +CKLLLVDKK+++ +++  +L+ A++  YPI+I+AE IEQE
Sbjct: 257 GYISPYFVTDSEKMSVEYDNCKLLLVDKKVTNARDLVGVLEDAIRGGYPILIIAEDIEQE 316

Query: 326 ALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVL 385
           ALA ++ NKLRG LK AA+KAP FGERKS YLDDIA LTGATVIRE+ GL+L+K GKEVL
Sbjct: 317 ALATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVL 376

Query: 386 GSATKVVISKDSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIA 445
           G+A+KVV++K+ T IV DG+T+EAV KRV+QI+ L+E  E+ + K+ LNERIA+LSG +A
Sbjct: 377 GNASKVVLTKEMTTIVGDGTTQEAVNKRVVQIRNLIEQAEQDYEKEKLNERIAKLSGGVA 436

Query: 446 ILQVGAQTQVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDE 505
           ++QVGAQT+ ELK+++LR+EDALNA+KAA+EEG+VVGGGC LLRL++KVDAIK+ LENDE
Sbjct: 437 VIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKDTLENDE 496

Query: 506 QMIGAEIFKRALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIM 565
           + +GAEI KRAL YP KLIA+NAGVNGSVV +KVL N+++ +GYNAAT  YEDLM AGI+
Sbjct: 497 EKVGAEIVKRALSYPLKLIAKNAGVNGSVVSEKVLANDNVKFGYNAATGKYEDLMAAGII 556

Query: 566 DPSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPEPIPRRTPMPSSG 612
           DP+KVVRCCLEHAASVAKTFL SD VVV+I EPEP+P   PM +SG
Sbjct: 557 DPTKVVRCCLEHAASVAKTFLMSDCVVVEIPEPEPVPAGNPMDNSG 593

BLAST of Lag0040218 vs. TAIR 10
Match: AT1G55490.1 (chaperonin 60 beta )

HSP 1 Score: 741.9 bits (1914), Expect = 4.7e-214
Identity = 378/576 (65.62%), Postives = 486/576 (84.38%), Query Frame = 0

Query: 37  ISPLSFPNLKSI-PKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKLQVGVDLVAEL 96
           +S  SF   +S+ P+P +  S++  A         KEL+FN+DG+ +++LQ GV+ +A+L
Sbjct: 30  LSSSSFGRRQSVCPRPRRSSSAIVCA--------AKELHFNKDGTTIRRLQAGVNKLADL 89

Query: 97  VGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAG 156
           VGVTLGPKGRNVVL++KYG P+IVNDG TV +E+ELEDP+EN+G KLVRQA AKTNDLAG
Sbjct: 90  VGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTNDLAG 149

Query: 157 DGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSREVENDEIAHV 216
           DG+TTSVVLAQG IAEG+KV+++G NPV I RGIEKTAKALV+ELK MS+EVE+ E+A V
Sbjct: 150 DGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVTELKKMSKEVEDSELADV 209

Query: 217 AAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDRGYLSPYFVTD 276
           AAVSAGN+  +GNMI++A+ +VGRKGVV +E+GKS +N+L +VEGMQFDRGY+SPYFVTD
Sbjct: 210 AAVSAGNNDEIGNMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDRGYISPYFVTD 269

Query: 277 RQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQEALAPVIRNKL 336
            +KM VEF +CKLLLVDKKI++ +++  +L+ A++  YPI+I+AE IEQEALA ++ NKL
Sbjct: 270 SEKMSVEFDNCKLLLVDKKITNARDLVGVLEDAIRGGYPILIIAEDIEQEALATLVVNKL 329

Query: 337 RGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSATKVVISK 396
           RG LK AA++AP FGERKS YLDDIA LTGATVIRE+ GL+L+K GKEVLG+A+KVV++K
Sbjct: 330 RGTLKIAALRAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLGNASKVVLTK 389

Query: 397 DSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAILQVGAQTQV 456
           +++ IV DGST++AV+KRV QI+ L+E  E+ + K+ LNERIA+LSG +A++QVGAQT+ 
Sbjct: 390 ETSTIVGDGSTQDAVKKRVTQIKNLIEQAEQDYEKEKLNERIAKLSGGVAVIQVGAQTET 449

Query: 457 ELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQMIGAEIFKR 516
           ELK+++LR+EDALNA+KAA+EEG+VVGGGC LLRL++KVDAIK  L+NDE+ +GA+I KR
Sbjct: 450 ELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKATLDNDEEKVGADIVKR 509

Query: 517 ALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSKVVRCCL 576
           AL YP KLIA+NAGVNGSVV +KVL N+++ +GYNAAT  YEDLM AGI+DP+KVVRCCL
Sbjct: 510 ALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFGYNAATGKYEDLMAAGIIDPTKVVRCCL 569

Query: 577 EHAASVAKTFLTSDAVVVDIKEPEPIPRRTPMPSSG 612
           EHAASVAKTFL SD VVV+IKEPEP+P   PM +SG
Sbjct: 570 EHAASVAKTFLMSDCVVVEIKEPEPVPVGNPMDNSG 597

BLAST of Lag0040218 vs. TAIR 10
Match: AT1G55490.2 (chaperonin 60 beta )

HSP 1 Score: 741.9 bits (1914), Expect = 4.7e-214
Identity = 378/576 (65.62%), Postives = 486/576 (84.38%), Query Frame = 0

Query: 37  ISPLSFPNLKSI-PKPNKLPSSLPPAWNPSPKSMPKELYFNRDGSALKKLQVGVDLVAEL 96
           +S  SF   +S+ P+P +  S++  A         KEL+FN+DG+ +++LQ GV+ +A+L
Sbjct: 30  LSSSSFGRRQSVCPRPRRSSSAIVCA--------AKELHFNKDGTTIRRLQAGVNKLADL 89

Query: 97  VGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAG 156
           VGVTLGPKGRNVVL++KYG P+IVNDG TV +E+ELEDP+EN+G KLVRQA AKTNDLAG
Sbjct: 90  VGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTNDLAG 149

Query: 157 DGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELKLMSREVENDEIAHV 216
           DG+TTSVVLAQG IAEG+KV+++G NPV I RGIEKTAKALV+ELK MS+EVE+ E+A V
Sbjct: 150 DGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVTELKKMSKEVEDSELADV 209

Query: 217 AAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDRGYLSPYFVTD 276
           AAVSAGN+  +GNMI++A+ +VGRKGVV +E+GKS +N+L +VEGMQFDRGY+SPYFVTD
Sbjct: 210 AAVSAGNNDEIGNMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDRGYISPYFVTD 269

Query: 277 RQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQEALAPVIRNKL 336
            +KM VEF +CKLLLVDKKI++ +++  +L+ A++  YPI+I+AE IEQEALA ++ NKL
Sbjct: 270 SEKMSVEFDNCKLLLVDKKITNARDLVGVLEDAIRGGYPILIIAEDIEQEALATLVVNKL 329

Query: 337 RGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSATKVVISK 396
           RG LK AA++AP FGERKS YLDDIA LTGATVIRE+ GL+L+K GKEVLG+A+KVV++K
Sbjct: 330 RGTLKIAALRAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLGNASKVVLTK 389

Query: 397 DSTLIVTDGSTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAILQVGAQTQV 456
           +++ IV DGST++AV+KRV QI+ L+E  E+ + K+ LNERIA+LSG +A++QVGAQT+ 
Sbjct: 390 ETSTIVGDGSTQDAVKKRVTQIKNLIEQAEQDYEKEKLNERIAKLSGGVAVIQVGAQTET 449

Query: 457 ELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQMIGAEIFKR 516
           ELK+++LR+EDALNA+KAA+EEG+VVGGGC LLRL++KVDAIK  L+NDE+ +GA+I KR
Sbjct: 450 ELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKATLDNDEEKVGADIVKR 509

Query: 517 ALCYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSKVVRCCL 576
           AL YP KLIA+NAGVNGSVV +KVL N+++ +GYNAAT  YEDLM AGI+DP+KVVRCCL
Sbjct: 510 ALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFGYNAATGKYEDLMAAGIIDPTKVVRCCL 569

Query: 577 EHAASVAKTFLTSDAVVVDIKEPEPIPRRTPMPSSG 612
           EHAASVAKTFL SD VVV+IKEPEP+P   PM +SG
Sbjct: 570 EHAASVAKTFLMSDCVVVEIKEPEPVPVGNPMDNSG 597