Lag0040189 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0040189
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cytochrome P450
Location	chr13: 2800315 .. 2801719 (+)
RNA-Seq Expression	Lag0040189
Synteny	Lag0040189

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAAAAATCCCCGGGTGGTCGGATCCCCGTGAGGCCCGCCCCAAAACAGGGTGGGGAATCCTTGGTTTGACCAGGTATGGGAGTCAAACCGGGGAATTTTTTTCAGTTCCCCAATCCGGTATCCCCATCCCTGACCCCAACCCCGAACCCGCCCCAAATGGGGATTCATGACTTCAACCCTGATTTCTCTAAACGAGGAATGGGGCGGGACTGAGGATGGGGAGTGCTTCCCCTCCTAACCCCACCACGCCCTGCGCCGTTGTCATTCCTAGTTTCAGGGACTAACCTTGTAATTGAACCTATTGTATTTCATCTTAGGAGTAGATGCTTGTAACTCTGAATATAAGTTATAACTTTGGATGTGTTAATTTTTGAATGAGTGCAGCAAGATTTTTAAATCTAAAGACGACGTGGAAGGAGAGAGGCTGGAAAAGAGAATGAAAGATTGCTTCATGGAAATAATCAAAGCTAGAGAAGAAAAGTCGATGAATGGGGAAGCAGATGGGTATGGGAATGACTTTTTGGGATTGTTGGTGAAGGCAAAGAACCACTCTGATAAATCACAGAGGATTTCTGTGGAAGATATTGTTGATGAATGCAAGACATTTTACCTTGCTGGCCAAGAAACCACCACCGTTTTGATTGCTTGGATAATGTTTCTTCTCGCCATGCACAAAGAATGGCAACATGAAGCAAGAAATGAAGTGTTGAAGATATTTGGAGATCAAAACCCTACATCAGAAGGCCTTCCAAAATTAACAACAGTAAGAATCAAATTGCATCATATTCAACCCATATATAAGATTTTAACTACATTATAAGCCTGATTAAAAAACGGGTTAAATAACTACAAACTTGTATCTCATGGTTATTAGGGACGTGCACATTTTCGAATCCACATCTAATGATTATTCGGTGTCATATCCATGATTTTGTTAGTGATCTCTGTTTGCAGATGACGATGATCATCAACGAATGCTTGAGGCTGTATCCACCGGCGATGACAGTATCGCGGCAAGTGGCGAAGGAAGTGAGACTGGGGAGGCTTGTTCTTCCTCCGACGCTTCAGCTGACGATCCCAACCATTGCAGTTCATCACGACAAGGCATTTTGGGGGGAAGACGCAGACGAGTTCAAACCAGAAAGATTCTCAGAAGGAGTGGCCAAAGCCATGGAAGAAGCCAACTCCGCCGGGTATCTCCCATTTGGATTGGGTGCTCGGAACTGCGTGGGAATGAACTTTGCAATGAACGAAGCCAAAATTGCAATGTCAATGATTCTACAGAAATATTCCTTCAACCTCTCGCCGGCCTACGCTCACACGCCCGCTCAGTTTCTCACCACTGCCCCCCAGCGGGGACTTCAACTCATTCTTCATTCGGTATCAGATCAAAAAGCCTGA

mRNA sequence

ATGACAAAAATCCCCGGGTGGTCGGATCCCCGTGAGGCCCGCCCCAAAACAGGGTGGGGAATCCTTGGTTTGACCAGCAAGATTTTTAAATCTAAAGACGACGTGGAAGGAGAGAGGCTGGAAAAGAGAATGAAAGATTGCTTCATGGAAATAATCAAAGCTAGAGAAGAAAAGTCGATGAATGGGGAAGCAGATGGGTATGGGAATGACTTTTTGGGATTGTTGGTGAAGGCAAAGAACCACTCTGATAAATCACAGAGGATTTCTGTGGAAGATATTGTTGATGAATGCAAGACATTTTACCTTGCTGGCCAAGAAACCACCACCGTTTTGATTGCTTGGATAATGTTTCTTCTCGCCATGCACAAAGAATGGCAACATGAAGCAAGAAATGAAGTGTTGAAGATATTTGGAGATCAAAACCCTACATCAGAAGGCCTTCCAAAATTAACAACAATGACGATGATCATCAACGAATGCTTGAGGCTGTATCCACCGGCGATGACAGTATCGCGGCAAGTGGCGAAGGAAGTGAGACTGGGGAGGCTTGTTCTTCCTCCGACGCTTCAGCTGACGATCCCAACCATTGCAGTTCATCACGACAAGGCATTTTGGGGGGAAGACGCAGACGAGTTCAAACCAGAAAGATTCTCAGAAGGAGTGGCCAAAGCCATGGAAGAAGCCAACTCCGCCGGGTATCTCCCATTTGGATTGGGTGCTCGGAACTGCGTGGGAATGAACTTTGCAATGAACGAAGCCAAAATTGCAATGTCAATGATTCTACAGAAATATTCCTTCAACCTCTCGCCGGCCTACGCTCACACGCCCGCTCAGTTTCTCACCACTGCCCCCCAGCGGGGACTTCAACTCATTCTTCATTCGGTATCAGATCAAAAAGCCTGA

Coding sequence (CDS)

ATGACAAAAATCCCCGGGTGGTCGGATCCCCGTGAGGCCCGCCCCAAAACAGGGTGGGGAATCCTTGGTTTGACCAGCAAGATTTTTAAATCTAAAGACGACGTGGAAGGAGAGAGGCTGGAAAAGAGAATGAAAGATTGCTTCATGGAAATAATCAAAGCTAGAGAAGAAAAGTCGATGAATGGGGAAGCAGATGGGTATGGGAATGACTTTTTGGGATTGTTGGTGAAGGCAAAGAACCACTCTGATAAATCACAGAGGATTTCTGTGGAAGATATTGTTGATGAATGCAAGACATTTTACCTTGCTGGCCAAGAAACCACCACCGTTTTGATTGCTTGGATAATGTTTCTTCTCGCCATGCACAAAGAATGGCAACATGAAGCAAGAAATGAAGTGTTGAAGATATTTGGAGATCAAAACCCTACATCAGAAGGCCTTCCAAAATTAACAACAATGACGATGATCATCAACGAATGCTTGAGGCTGTATCCACCGGCGATGACAGTATCGCGGCAAGTGGCGAAGGAAGTGAGACTGGGGAGGCTTGTTCTTCCTCCGACGCTTCAGCTGACGATCCCAACCATTGCAGTTCATCACGACAAGGCATTTTGGGGGGAAGACGCAGACGAGTTCAAACCAGAAAGATTCTCAGAAGGAGTGGCCAAAGCCATGGAAGAAGCCAACTCCGCCGGGTATCTCCCATTTGGATTGGGTGCTCGGAACTGCGTGGGAATGAACTTTGCAATGAACGAAGCCAAAATTGCAATGTCAATGATTCTACAGAAATATTCCTTCAACCTCTCGCCGGCCTACGCTCACACGCCCGCTCAGTTTCTCACCACTGCCCCCCAGCGGGGACTTCAACTCATTCTTCATTCGGTATCAGATCAAAAAGCCTGA

Protein sequence

MTKIPGWSDPREARPKTGWGILGLTSKIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNHSDKSQRISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQNPTSEGLPKLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHDKAFWGEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKYSFNLSPAYAHTPAQFLTTAPQRGLQLILHSVSDQKA

Homology

[Close]

BLAST of Lag0040189 vs. NCBI nr
Match: XP_022945316.1 (cytochrome P450 CYP749A22-like [Cucurbita moschata])

HSP 1 Score: 454.5 bits (1168), Expect = 7.0e-124
Identity = 229/275 (83.27%), Postives = 243/275 (88.36%), Query Frame = 0


Query: 26  SKIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNHSDKS 85
           SKIFKSKDDVEGERLEKRM++ FME+IK+REEK MNGEA+GYGNDFLGLLVKAKN  + S
Sbjct: 246 SKIFKSKDDVEGERLEKRMEEWFMEMIKSREEKFMNGEAEGYGNDFLGLLVKAKNDPENS 305

Query: 86  QRISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQNPTSE 145
            RIS + IV ECKTFY AG ETT VLIAW MFLLAMHKEWQ EARNEVL+IFG +NP+SE
Sbjct: 306 HRISADVIVAECKTFYFAGHETTNVLIAWTMFLLAMHKEWQDEARNEVLEIFGTKNPSSE 365

Query: 146 GLPKLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHDKAFW 205
           GLPKL TMTMIINECLRLYPPAMTVSRQV KEVRLGRL LP TLQLT+PTIAVHHDKAFW
Sbjct: 366 GLPKLRTMTMIINECLRLYPPAMTVSRQVEKEVRLGRLRLPATLQLTVPTIAVHHDKAFW 425

Query: 206 GEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKYS 265
           GEDA EFKPERF EGV K ME  NSAGYLPFGLG R+CVGM+FAMNEAKIAMSMILQKYS
Sbjct: 426 GEDAHEFKPERFGEGVGKVMER-NSAGYLPFGLGPRSCVGMSFAMNEAKIAMSMILQKYS 485

Query: 266 FNLSPAYAHTPAQFLTTAPQRGLQLILHSVSDQKA 301
             LSPAYAH PAQFLTT PQ+GL LILHSVSD KA
Sbjct: 486 LALSPAYAHMPAQFLTTCPQQGLHLILHSVSDHKA 519

[Close]

BLAST of Lag0040189 vs. NCBI nr
Match: XP_023543007.1 (cytochrome P450 CYP749A22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448.4 bits (1152), Expect = 5.0e-122
Identity = 226/275 (82.18%), Postives = 243/275 (88.36%), Query Frame = 0


Query: 26  SKIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNHSDKS 85
           SKIFKSKDDVEGERLEKRM++ FMEIIK+REEK MNGEA+GYGNDFLGLLVKAKN  + S
Sbjct: 246 SKIFKSKDDVEGERLEKRMEEWFMEIIKSREEKFMNGEAEGYGNDFLGLLVKAKNDPENS 305

Query: 86  QRISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQNPTSE 145
            RIS + IV ECKTFY AG ETT VLIAW MFLLAMH+EWQ EARNEVL+IFG +NP++E
Sbjct: 306 LRISADVIVAECKTFYFAGHETTNVLIAWTMFLLAMHQEWQDEARNEVLEIFGAKNPSAE 365

Query: 146 GLPKLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHDKAFW 205
           GLPKL TMTMIINECLRLYPPAMTVSRQV KEVRLGRL LP TLQ+T+PTIAVHHDKAFW
Sbjct: 366 GLPKLRTMTMIINECLRLYPPAMTVSRQVEKEVRLGRLRLPATLQVTVPTIAVHHDKAFW 425

Query: 206 GEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKYS 265
           GEDA EFKPERF EGV KA+E  NSAGYLPFGLG R+CVGM+FAMNEAKIAMSMILQKYS
Sbjct: 426 GEDAHEFKPERFGEGVGKAIER-NSAGYLPFGLGPRSCVGMSFAMNEAKIAMSMILQKYS 485

Query: 266 FNLSPAYAHTPAQFLTTAPQRGLQLILHSVSDQKA 301
             LSPAYAH PAQFLT  PQ+GL LILHSVSD KA
Sbjct: 486 LALSPAYAHMPAQFLTICPQQGLHLILHSVSDHKA 519

[Close]

BLAST of Lag0040189 vs. NCBI nr
Match: XP_022149052.1 (cytochrome P450 CYP749A22-like [Momordica charantia] >XP_022149053.1 cytochrome P450 CYP749A22-like [Momordica charantia])

HSP 1 Score: 447.6 bits (1150), Expect = 8.5e-122
Identity = 219/275 (79.64%), Postives = 244/275 (88.73%), Query Frame = 0


Query: 27  KIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNHSDKSQ 86
           KIFKSKDDVEGERLEKRMKDCFME+IK+REEK +NGEADGYGNDFLGLLVKAKN  ++SQ
Sbjct: 246 KIFKSKDDVEGERLEKRMKDCFMEVIKSREEKFINGEADGYGNDFLGLLVKAKNDPEESQ 305

Query: 87  RISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQNPTSEG 146
           RISV+DIVDECKTFY AG ETT+VL+AW MFLLA+HK+WQ +AR+EV K+FGD+NPTSEG
Sbjct: 306 RISVDDIVDECKTFYFAGHETTSVLLAWTMFLLALHKQWQDQARDEVFKVFGDKNPTSEG 365

Query: 147 LPKLTTMTMIINECLRLYPPAMTVSRQV-AKEVRLGRLVLPPTLQLTIPTIAVHHDKAFW 206
           LPKL TMTMI+ ECLRLYPPAMTV RQV  KEVRLG LVLP T+++TIPT AVHHD A+W
Sbjct: 366 LPKLKTMTMILYECLRLYPPAMTVGRQVMEKEVRLGSLVLPATIRVTIPTAAVHHDTAYW 425

Query: 207 GEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKYS 266
           GEDA EFKPERF+EGVAKA+E  NSA YLPFGLG R+CVGMNFAM EAKI MSMILQ+YS
Sbjct: 426 GEDASEFKPERFAEGVAKAIER-NSAAYLPFGLGGRSCVGMNFAMKEAKIGMSMILQRYS 485

Query: 267 FNLSPAYAHTPAQFLTTAPQRGLQLILHSVSDQKA 301
           F LSPAYAH+P QFLT  PQ GLQLILHS+SD  A
Sbjct: 486 FTLSPAYAHSPVQFLTICPQHGLQLILHSISDHTA 519

[Close]

BLAST of Lag0040189 vs. NCBI nr
Match: XP_038891307.1 (cytochrome P450 CYP749A22-like [Benincasa hispida])

HSP 1 Score: 439.1 bits (1128), Expect = 3.0e-119
Identity = 219/275 (79.64%), Postives = 240/275 (87.27%), Query Frame = 0


Query: 22  LGLTSKIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNH 81
           L + SKI KSKDDVEGERLEKRMK+CF+EII+ REEK  NGE +GYGNDFLG+LVKAKN 
Sbjct: 245 LPIISKILKSKDDVEGERLEKRMKECFVEIIRGREEKMKNGEGEGYGNDFLGMLVKAKNE 304

Query: 82  SDKSQRISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQN 141
            +KS+RIS++ IV ECKTFY AG ETT VLI WI+FLLA+HKEWQ EARNEVL+IFG  N
Sbjct: 305 DEKSERISMDVIVAECKTFYFAGHETTNVLITWIVFLLAIHKEWQEEARNEVLRIFGHNN 364

Query: 142 PTSEGLPKLTTMTMIINECLRLYPPAMTVSRQVA-KEVRLGRLVLPPTLQLTIPTIAVHH 201
           PTSEGL KL TMTMIINE LRLYPPAMTVSRQV  KEVRLG L+LP TLQL IPT+AVHH
Sbjct: 365 PTSEGLSKLKTMTMIINETLRLYPPAMTVSRQVVEKEVRLGSLLLPTTLQLMIPTVAVHH 424

Query: 202 DKAFWGEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMI 261
           DKAFWGEDA EFKPERFSEG++K ++  NSAGYLPFGLG RNCVGMNFAMNEAKIAMSMI
Sbjct: 425 DKAFWGEDAHEFKPERFSEGISKTIQR-NSAGYLPFGLGPRNCVGMNFAMNEAKIAMSMI 484

Query: 262 LQKYSFNLSPAYAHTPAQFLTTAPQRGLQLILHSV 296
           LQKYSF LSPAYAHTP QFLTT PQ+GLQ+IL SV
Sbjct: 485 LQKYSFTLSPAYAHTPVQFLTTCPQQGLQVILRSV 518

[Close]

BLAST of Lag0040189 vs. NCBI nr
Match: XP_038892924.1 (cytochrome P450 CYP749A22-like isoform X1 [Benincasa hispida])

HSP 1 Score: 437.6 bits (1124), Expect = 8.8e-119
Identity = 217/279 (77.78%), Postives = 237/279 (84.95%), Query Frame = 0


Query: 22  LGLTSKIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNH 81
           L + SKI KSKDD EGERLEKRMKDCFMEIIK REEK  NGEADGYGNDFLGLLVKAKN 
Sbjct: 241 LPIISKILKSKDDYEGERLEKRMKDCFMEIIKGREEKLRNGEADGYGNDFLGLLVKAKND 300

Query: 82  SDKSQRISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQN 141
            + SQRISVEDIVDECKTFY AG ETT VL+AW MFLLA+HKEWQ EARNEVLK+FGD+N
Sbjct: 301 PEISQRISVEDIVDECKTFYFAGHETTNVLLAWTMFLLALHKEWQEEARNEVLKVFGDKN 360

Query: 142 PTSEGLPKLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHD 201
           PT EGLPKL  M +IINECLRLYPPAMTVSR V KEVRLG LV+P   +L IPT+AVHHD
Sbjct: 361 PTLEGLPKLRMMMIIINECLRLYPPAMTVSRLVEKEVRLGNLVVPTMTKLVIPTVAVHHD 420

Query: 202 KAFWGEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMIL 261
             FWGEDA EFKPERF EGV KA  E+NSA Y+PFGLG RNCVGMNFAMNEAKIAMSMIL
Sbjct: 421 MTFWGEDAHEFKPERFLEGVGKA-TESNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMIL 480

Query: 262 QKYSFNLSPAYAHTPAQFLTTAPQRGLQLILHSVSDQKA 301
           Q+YSF+LSPAY H PAQ LT +PQ G+Q+IL+S++  KA
Sbjct: 481 QRYSFDLSPAYVHMPAQLLTISPQNGVQVILNSITYHKA 518

[Close]

BLAST of Lag0040189 vs. ExPASy Swiss-Prot
Match: H2DH17 (Cytochrome P450 CYP749A22 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 4.6e-86
Identity = 149/268 (55.60%), Postives = 203/268 (75.75%), Query Frame = 0


Query: 26  SKIFKSKDDVEGERLEKRMKDCFMEIIKARE-EKSMNGEADGYGNDFLGLLVKAKNHSDK 85
           S  +KS D++E E+L++ + D  + I++ RE E +M+GE   +G DFLGLL+KA N +D+
Sbjct: 255 SLFYKSNDEIEAEKLDQGLYDSILRIMEKREKESTMSGEVGSFGTDFLGLLMKAMNDADE 314

Query: 86  SQRISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQNPTS 145
             RI+ +D+VDECKTFY+AGQETTT L+AW++FLL +H +WQ +AR EVL +FG + P S
Sbjct: 315 KNRITAQDVVDECKTFYVAGQETTTTLLAWVIFLLGIHTDWQEKARQEVLNLFGQEIPNS 374

Query: 146 EGLPKLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHDKAF 205
           +GL KL T+ MIINE LRLYPP + ++R+V +E + G+L LP  + + +PT+A+HHD+  
Sbjct: 375 DGLAKLKTVNMIINETLRLYPPVIFLTRKVKEETKFGKLTLPANVHIVVPTLALHHDEQI 434

Query: 206 WGEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKY 265
           WG+DA  FKPERFS+GVAKA    N+A + PFGLG R+CVG+NFA NEAKIA++MILQ Y
Sbjct: 435 WGDDALLFKPERFSQGVAKATNN-NAAAFFPFGLGPRSCVGLNFATNEAKIALAMILQCY 494

Query: 266 SFNLSPAYAHTPAQFLTTAPQRGLQLIL 293
           SF LSP Y H+P Q LT  PQ GLQ++L
Sbjct: 495 SFALSPTYIHSPVQILTVRPQHGLQVML 521

[Close]

BLAST of Lag0040189 vs. ExPASy Swiss-Prot
Match: H1A981 (11-oxo-beta-amyrin 30-oxidase OS=Medicago truncatula OX=3880 GN=CYP72A63 PE=1 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 3.1e-66
Identity = 126/284 (44.37%), Postives = 185/284 (65.14%), Query Frame = 0


Query: 19  WGILGLTSKIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKA 78
           WG+L  T+K        + + +++ + D    II+ RE+   NGE     +D LG+L+++
Sbjct: 253 WGLLATTTK-------TKMKEIDREIHDSLEGIIEKREKALKNGETT--NDDLLGILLQS 312

Query: 79  KNHSDKSQR-------ISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARN 138
            NH++K  +       ++ +D++DECK FYLAGQETT+ L+ W M LL  + EWQ  AR 
Sbjct: 313 -NHAEKQGQGNSKNIGMTTQDVIDECKLFYLAGQETTSSLLVWTMVLLGRYPEWQARARE 372

Query: 139 EVLKIFGDQNPTSEGLPKLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQL 198
           EVL++FG+QNP +EGL +L  +TMI+ E LRL+PP +  +R + K+++LG L+LP   Q+
Sbjct: 373 EVLQVFGNQNPNNEGLSQLKIVTMILYEVLRLFPPLIYFNRALRKDLKLGNLLLPEGTQI 432

Query: 199 TIPTIAVHHDKAFWGEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMN 258
           ++P + +H D   WG+DA EFKPERF+EG+AKA +      Y PFG G R C+G NFA+ 
Sbjct: 433 SLPILLIHQDHDLWGDDAKEFKPERFAEGIAKATK--GQVSYFPFGWGPRICLGQNFALL 492

Query: 259 EAKIAMSMILQKYSFNLSPAYAHTPAQFLTTAPQRGLQLILHSV 296
           EAKIA+S++LQ +SF LSP Y H P   LT  P+ G  +ILH +
Sbjct: 493 EAKIAVSLLLQNFSFELSPNYVHVPTTVLTLQPKNGASIILHKL 524

[Close]

BLAST of Lag0040189 vs. ExPASy Swiss-Prot
Match: H1A988 (11-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP72A154 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.2e-65
Identity = 121/264 (45.83%), Postives = 178/264 (67.42%), Query Frame = 0


Query: 38  ERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAK------NHSDKSQRISVE 97
           + +E+ ++D    II+ RE+   +G++    +D LG+L+++       + + KS  ++ +
Sbjct: 264 KEIERGIRDSLEGIIRKREKALKSGKST--DDDLLGILLQSNHIENKGDENSKSAGMTTQ 323

Query: 98  DIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQNPTSEGLPKLT 157
           ++++ECK FYLAGQETT  L+AW M LL  H EWQ  AR EVL++FG+QNP  EGL +L 
Sbjct: 324 EVMEECKLFYLAGQETTAALLAWTMVLLGKHPEWQARARQEVLQVFGNQNPNFEGLGRLK 383

Query: 158 TMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHDKAFWGEDADE 217
            +TMI+ E LRLYPP + ++R + K+++LG L+LP  +Q+++P + +HHD+  WG DA E
Sbjct: 384 IVTMILYEVLRLYPPGIYLTRALRKDLKLGNLLLPAGVQVSVPILLIHHDEGIWGNDAKE 443

Query: 218 FKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKYSFNLSPA 277
           F PERF+EG+AKA +      Y PFG G R CVG NFA+ EAKI +S++LQ +SF LSP 
Sbjct: 444 FNPERFAEGIAKATK--GQVCYFPFGWGPRICVGQNFALLEAKIVLSLLLQNFSFELSPT 503

Query: 278 YAHTPAQFLTTAPQRGLQLILHSV 296
           YAH P   LT  P+ G  +ILH +
Sbjct: 504 YAHVPTTVLTLQPKHGAPIILHKL 523

[Close]

BLAST of Lag0040189 vs. ExPASy Swiss-Prot
Match: Q9LUC6 (Cytochrome P450 72A14 OS=Arabidopsis thaliana OX=3702 GN=CYP72A14 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.7e-64
Identity = 119/254 (46.85%), Postives = 169/254 (66.54%), Query Frame = 0


Query: 42  KRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNHSDKSQRISVEDIVDECKTFY 101
           + ++D    II  RE    +GEA     D LG+L+++     +   +S ED+++ECK FY
Sbjct: 263 REIQDILRGIINKRERARESGEAP--SEDLLGILLESNLGQTEGNGMSTEDMMEECKLFY 322

Query: 102 LAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQNPTSEGLPKLTTMTMIINECL 161
           LAGQETT+VL+ W M LL+ H++WQ  AR EV ++FGD+ P +EGL +L  MTMI+ E L
Sbjct: 323 LAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDTEGLNQLKVMTMILYEVL 382

Query: 162 RLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHDKAFWGEDADEFKPERFSEGV 221
           RLYPP + ++R + KE++LG L LP  +Q+++P + VH D   WG DA EFKPERF +G+
Sbjct: 383 RLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFKPERFKDGL 442

Query: 222 AKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKYSFNLSPAYAHTPAQFLT 281
           +KA +  N   + PF  G R C+G NF + EAK+AMS+ILQ++SF LSP+Y H P   +T
Sbjct: 443 SKATK--NQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHAPYTIIT 502

Query: 282 TAPQRGLQLILHSV 296
             PQ G  L+LH +
Sbjct: 503 LYPQFGAHLMLHKL 512

[Close]

BLAST of Lag0040189 vs. ExPASy Swiss-Prot
Match: Q9LUC8 (Cytochrome P450 72A13 OS=Arabidopsis thaliana OX=3702 GN=CYP72A13 PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 5.5e-63
Identity = 119/269 (44.24%), Postives = 174/269 (64.68%), Query Frame = 0


Query: 27  KIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNHSDKSQ 86
           + F +K +   +   + +K     I+  R      GEA    +D LG+L+++     K  
Sbjct: 248 RYFPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGEAP--SDDLLGILLESNLGQTKGN 307

Query: 87  RISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQNPTSEG 146
            +S E++++ECK FY AGQETTTVL+ W M LL+ H++WQ  AR EV ++FGD+ P +EG
Sbjct: 308 GMSTEELMEECKLFYFAGQETTTVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEG 367

Query: 147 LPKLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHDKAFWG 206
           L +L  MTMI+ E LRLYPP + ++R + KE++LG L LP  +Q+++P + +  D+  WG
Sbjct: 368 LNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISLPILLIQRDRELWG 427

Query: 207 EDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKYSF 266
            DA EFKP+RF +G++KA +  N   + PF  G R C+G NFA+ EAK+AM++IL+K+SF
Sbjct: 428 NDAGEFKPDRFKDGLSKATK--NQVSFFPFAWGPRICIGQNFALLEAKMAMTLILRKFSF 487

Query: 267 NLSPAYAHTPAQFLTTAPQRGLQLILHSV 296
            LSP+Y H P   LTT PQ G  LILH +
Sbjct: 488 ELSPSYVHAPYTVLTTHPQFGAPLILHKL 512

[Close]

BLAST of Lag0040189 vs. ExPASy TrEMBL
Match: A0A6J1G0I3 (cytochrome P450 CYP749A22-like OS=Cucurbita moschata OX=3662 GN=LOC111449593 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.4e-124
Identity = 229/275 (83.27%), Postives = 243/275 (88.36%), Query Frame = 0


Query: 26  SKIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNHSDKS 85
           SKIFKSKDDVEGERLEKRM++ FME+IK+REEK MNGEA+GYGNDFLGLLVKAKN  + S
Sbjct: 246 SKIFKSKDDVEGERLEKRMEEWFMEMIKSREEKFMNGEAEGYGNDFLGLLVKAKNDPENS 305

Query: 86  QRISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQNPTSE 145
            RIS + IV ECKTFY AG ETT VLIAW MFLLAMHKEWQ EARNEVL+IFG +NP+SE
Sbjct: 306 HRISADVIVAECKTFYFAGHETTNVLIAWTMFLLAMHKEWQDEARNEVLEIFGTKNPSSE 365

Query: 146 GLPKLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHDKAFW 205
           GLPKL TMTMIINECLRLYPPAMTVSRQV KEVRLGRL LP TLQLT+PTIAVHHDKAFW
Sbjct: 366 GLPKLRTMTMIINECLRLYPPAMTVSRQVEKEVRLGRLRLPATLQLTVPTIAVHHDKAFW 425

Query: 206 GEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKYS 265
           GEDA EFKPERF EGV K ME  NSAGYLPFGLG R+CVGM+FAMNEAKIAMSMILQKYS
Sbjct: 426 GEDAHEFKPERFGEGVGKVMER-NSAGYLPFGLGPRSCVGMSFAMNEAKIAMSMILQKYS 485

Query: 266 FNLSPAYAHTPAQFLTTAPQRGLQLILHSVSDQKA 301
             LSPAYAH PAQFLTT PQ+GL LILHSVSD KA
Sbjct: 486 LALSPAYAHMPAQFLTTCPQQGLHLILHSVSDHKA 519

[Close]

BLAST of Lag0040189 vs. ExPASy TrEMBL
Match: A0A6J1D6R8 (cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017564 PE=3 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 4.1e-122
Identity = 219/275 (79.64%), Postives = 244/275 (88.73%), Query Frame = 0


Query: 27  KIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNHSDKSQ 86
           KIFKSKDDVEGERLEKRMKDCFME+IK+REEK +NGEADGYGNDFLGLLVKAKN  ++SQ
Sbjct: 246 KIFKSKDDVEGERLEKRMKDCFMEVIKSREEKFINGEADGYGNDFLGLLVKAKNDPEESQ 305

Query: 87  RISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQNPTSEG 146
           RISV+DIVDECKTFY AG ETT+VL+AW MFLLA+HK+WQ +AR+EV K+FGD+NPTSEG
Sbjct: 306 RISVDDIVDECKTFYFAGHETTSVLLAWTMFLLALHKQWQDQARDEVFKVFGDKNPTSEG 365

Query: 147 LPKLTTMTMIINECLRLYPPAMTVSRQV-AKEVRLGRLVLPPTLQLTIPTIAVHHDKAFW 206
           LPKL TMTMI+ ECLRLYPPAMTV RQV  KEVRLG LVLP T+++TIPT AVHHD A+W
Sbjct: 366 LPKLKTMTMILYECLRLYPPAMTVGRQVMEKEVRLGSLVLPATIRVTIPTAAVHHDTAYW 425

Query: 207 GEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKYS 266
           GEDA EFKPERF+EGVAKA+E  NSA YLPFGLG R+CVGMNFAM EAKI MSMILQ+YS
Sbjct: 426 GEDASEFKPERFAEGVAKAIER-NSAAYLPFGLGGRSCVGMNFAMKEAKIGMSMILQRYS 485

Query: 267 FNLSPAYAHTPAQFLTTAPQRGLQLILHSVSDQKA 301
           F LSPAYAH+P QFLT  PQ GLQLILHS+SD  A
Sbjct: 486 FTLSPAYAHSPVQFLTICPQHGLQLILHSISDHTA 519

[Close]

BLAST of Lag0040189 vs. ExPASy TrEMBL
Match: A0A1S3BEM5 (cytochrome P450 CYP749A22-like OS=Cucumis melo OX=3656 GN=LOC103489194 PE=3 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 1.3e-112
Identity = 210/278 (75.54%), Postives = 236/278 (84.89%), Query Frame = 0


Query: 22  LGLTSKIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNH 81
           L + SKI KSKDD+EGERLEKRM +CF EII+ REEKS  GE  GYGNDFLG+LVKAKN 
Sbjct: 254 LPIISKILKSKDDIEGERLEKRMNECFAEIIRGREEKSKKGE--GYGNDFLGMLVKAKNE 313

Query: 82  SDKSQRISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFG-DQ 141
            +KS+RI+++ IV ECKTFY AG ETT VLI WI+FLLA+HKEWQ +ARNEV +IFG + 
Sbjct: 314 GEKSERITMDVIVAECKTFYFAGHETTNVLITWIIFLLAIHKEWQEQARNEVFRIFGHNN 373

Query: 142 NPTSEGLPKLTTMTMIINECLRLYPPAMTVSRQVA-KEVRLGRLVLPPTLQLTIPTIAVH 201
           NPT E L KL  MTMIINE LRLYPPAMTVSRQV  KEV+LG LVLP +LQLTIPTIAVH
Sbjct: 374 NPTYEALSKLKIMTMIINETLRLYPPAMTVSRQVVEKEVKLGSLVLPTSLQLTIPTIAVH 433

Query: 202 HDKAFWGEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSM 261
           HDK FWGED  EFKPERFSEGV+K +E  NSAGYLPFGLG RNCVGMNFA+NEAK+AMS+
Sbjct: 434 HDKEFWGEDVHEFKPERFSEGVSKTIER-NSAGYLPFGLGPRNCVGMNFAINEAKVAMSI 493

Query: 262 ILQKYSFNLSPAYAHTPAQFLTTAPQRGLQLILHSVSD 298
           ILQKYSF LSPAYAHTP QFLTT PQ+GLQ+ILHS+S+
Sbjct: 494 ILQKYSFTLSPAYAHTPVQFLTTCPQQGLQVILHSISN 528

[Close]

BLAST of Lag0040189 vs. ExPASy TrEMBL
Match: A0A1S3BF46 (cytochrome P450 CYP749A22-like OS=Cucumis melo OX=3656 GN=LOC103489195 PE=3 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 1.3e-112
Identity = 203/276 (73.55%), Postives = 228/276 (82.61%), Query Frame = 0


Query: 22  LGLTSKIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNH 81
           L + SKI KSKDD EGERLEKR+KDCFMEIIK REEK  N EA  YGNDFLGLL+KAKN 
Sbjct: 241 LPIISKILKSKDDYEGERLEKRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKND 300

Query: 82  SDKSQRISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQN 141
            + SQ IS+EDIVDECKTFY AG ETT VL+AW M LLA+HKEWQ +ARNEV  +FG  +
Sbjct: 301 PETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNH 360

Query: 142 PTSEGLPKLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHD 201
           PT EGLPKL  M MIINECLRLYPPAMT++R+V KEVRLG LV+P T  L IPT+AVHHD
Sbjct: 361 PTLEGLPKLKAMAMIINECLRLYPPAMTIARRVEKEVRLGNLVVPTTTLLAIPTVAVHHD 420

Query: 202 KAFWGEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMIL 261
             FWG+DA EFKPERFSEG+ KA E+ NSA YLPFGLG RNCVGMNFA NEAKIAMSMIL
Sbjct: 421 TKFWGDDAHEFKPERFSEGIGKATEK-NSAAYLPFGLGPRNCVGMNFATNEAKIAMSMIL 480

Query: 262 QKYSFNLSPAYAHTPAQFLTTAPQRGLQLILHSVSD 298
           Q+YSF+LSPAYAH PAQ LT  PQ G+Q+IL+S++D
Sbjct: 481 QRYSFSLSPAYAHMPAQLLTICPQNGVQVILNSIAD 515

[Close]

BLAST of Lag0040189 vs. ExPASy TrEMBL
Match: A0A6J1IKI4 (cytochrome P450 CYP749A22-like OS=Cucurbita maxima OX=3661 GN=LOC111474764 PE=3 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 5.1e-112
Identity = 199/274 (72.63%), Postives = 231/274 (84.31%), Query Frame = 0


Query: 22  LGLTSKIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNH 81
           L + SKIFKSKDDVEGERLEKRM++CF+EII+ REEK MNGEA+GYGNDFLGLLVKAKN 
Sbjct: 73  LPIISKIFKSKDDVEGERLEKRMRECFIEIIRTREEKLMNGEAEGYGNDFLGLLVKAKNE 132

Query: 82  SDKSQRISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQN 141
            +K+QR+SV+DIVDECKTFY AG ETT  L+AW MFLLA+H+EWQ EAR EV K+ GD+N
Sbjct: 133 PEKAQRLSVDDIVDECKTFYFAGHETTNALLAWTMFLLALHREWQEEARKEVFKVLGDKN 192

Query: 142 PTSEGLPKLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHD 201
           PT E LPKL TM MIINECLRLYPPAMT+SRQV KE RLG LVLP  +++ IPT+AV H+
Sbjct: 193 PTMESLPKLRTMNMIINECLRLYPPAMTLSRQVQKETRLGSLVLPSRIRVLIPTLAVQHN 252

Query: 202 KAFWGEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMIL 261
              WGEDAD FKPERF+EGV KA  E NS  YLPFGLG R+CVGMNFA+NEAKIA+S+IL
Sbjct: 253 TELWGEDADVFKPERFAEGVGKA-TETNSGAYLPFGLGPRSCVGMNFALNEAKIALSIIL 312

Query: 262 QKYSFNLSPAYAHTPAQFLTTAPQRGLQLILHSV 296
           Q++SF LSP+Y+H+P QFLT  PQ G+QLILH V
Sbjct: 313 QRFSFTLSPSYSHSPIQFLTINPQHGVQLILHPV 345

[Close]

BLAST of Lag0040189 vs. TAIR 10
Match: AT3G14630.1 (cytochrome P450, family 72, subfamily A, polypeptide 9 )

HSP 1 Score: 250.0 bits (637), Expect = 2.4e-66
Identity = 121/267 (45.32%), Postives = 179/267 (67.04%), Query Frame = 0


Query: 29  FKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNHSDKSQRI 88
           F +K++   + + K ++     II  RE+    GEA    +D LG+L+K+ +   K   +
Sbjct: 246 FPTKNNRRMKTIVKEIQVILRGIISHREKARDAGEAP--SDDLLGILLKSNSEQSKGNGL 305

Query: 89  SVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQNPTSEGLP 148
           ++E+I++ECK FY AGQETT+VL+AW M LL+ H++WQ  AR EV+++FG   P  +G+ 
Sbjct: 306 NMEEIMEECKLFYFAGQETTSVLLAWTMVLLSQHQDWQARAREEVMQVFGHNKPDLQGIN 365

Query: 149 KLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHDKAFWGED 208
           +L  MTMII E LRLYPP + ++R   KE++LG + LP  +Q+ +P + +H D   WG+D
Sbjct: 366 QLKVMTMIIYEVLRLYPPVIQMNRATHKEIKLGDMTLPGGIQVHMPVLLIHRDTKLWGDD 425

Query: 209 ADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKYSFNL 268
           A EFKPERF +G+AKA +  N   +LPFG G R C+G NFA+ EAK+A+++ILQ++SF L
Sbjct: 426 AAEFKPERFKDGIAKATK--NQVCFLPFGWGPRICIGQNFALLEAKMALALILQRFSFEL 485

Query: 269 SPAYAHTPAQFLTTAPQRGLQLILHSV 296
           SP+Y H+P +  T  PQ G  LILH +
Sbjct: 486 SPSYVHSPYRVFTIHPQCGAHLILHKL 508

[Close]

BLAST of Lag0040189 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 247.7 bits (631), Expect = 1.2e-65
Identity = 123/271 (45.39%), Postives = 174/271 (64.21%), Query Frame = 0


Query: 26  SKIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNHSDKS 85
           S+ + +K +   + +++ +      I+  RE+    GE     +D LG+L+++ +   + 
Sbjct: 246 SRFYPTKSNRRMKAIDREVDVILRGIVSKREKAREAGEP--ANDDLLGILLESNSEESQG 305

Query: 86  QRISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQN-PTS 145
             +SVED++ ECK FY AGQETT+VL+ W M LL+ H++WQ  AR EV+++ G+ N P  
Sbjct: 306 NGMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQVLGENNKPDM 365

Query: 146 EGLPKLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHDKAF 205
           E L  L  MTMI NE LRLYPP   + R V KE++LG L LP  +Q+ +PTI V  D   
Sbjct: 366 ESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTILVQRDTEL 425

Query: 206 WGEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKY 265
           WG+DA +FKPERF +G++KA +  N   + PFG G R C+G NFAM EAK+AM++ILQK+
Sbjct: 426 WGDDAADFKPERFRDGLSKATK--NQVSFFPFGWGPRICIGQNFAMLEAKMAMALILQKF 485

Query: 266 SFNLSPAYAHTPAQFLTTAPQRGLQLILHSV 296
           SF LSP+Y H P   +TT PQ G  LILH +
Sbjct: 486 SFELSPSYVHAPQTVMTTRPQFGAHLILHKL 512

[Close]

BLAST of Lag0040189 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 247.7 bits (631), Expect = 1.2e-65
Identity = 119/254 (46.85%), Postives = 169/254 (66.54%), Query Frame = 0


Query: 42  KRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNHSDKSQRISVEDIVDECKTFY 101
           + ++D    II  RE    +GEA     D LG+L+++     +   +S ED+++ECK FY
Sbjct: 263 REIQDILRGIINKRERARESGEAP--SEDLLGILLESNLGQTEGNGMSTEDMMEECKLFY 322

Query: 102 LAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQNPTSEGLPKLTTMTMIINECL 161
           LAGQETT+VL+ W M LL+ H++WQ  AR EV ++FGD+ P +EGL +L  MTMI+ E L
Sbjct: 323 LAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDTEGLNQLKVMTMILYEVL 382

Query: 162 RLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHDKAFWGEDADEFKPERFSEGV 221
           RLYPP + ++R + KE++LG L LP  +Q+++P + VH D   WG DA EFKPERF +G+
Sbjct: 383 RLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFKPERFKDGL 442

Query: 222 AKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKYSFNLSPAYAHTPAQFLT 281
           +KA +  N   + PF  G R C+G NF + EAK+AMS+ILQ++SF LSP+Y H P   +T
Sbjct: 443 SKATK--NQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHAPYTIIT 502

Query: 282 TAPQRGLQLILHSV 296
             PQ G  L+LH +
Sbjct: 503 LYPQFGAHLMLHKL 512

[Close]

BLAST of Lag0040189 vs. TAIR 10
Match: AT3G14660.1 (cytochrome P450, family 72, subfamily A, polypeptide 13 )

HSP 1 Score: 242.7 bits (618), Expect = 3.9e-64
Identity = 119/269 (44.24%), Postives = 174/269 (64.68%), Query Frame = 0


Query: 27  KIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNHSDKSQ 86
           + F +K +   +   + +K     I+  R      GEA    +D LG+L+++     K  
Sbjct: 248 RYFPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGEAP--SDDLLGILLESNLGQTKGN 307

Query: 87  RISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQNPTSEG 146
            +S E++++ECK FY AGQETTTVL+ W M LL+ H++WQ  AR EV ++FGD+ P +EG
Sbjct: 308 GMSTEELMEECKLFYFAGQETTTVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEG 367

Query: 147 LPKLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGRLVLPPTLQLTIPTIAVHHDKAFWG 206
           L +L  MTMI+ E LRLYPP + ++R + KE++LG L LP  +Q+++P + +  D+  WG
Sbjct: 368 LNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISLPILLIQRDRELWG 427

Query: 207 EDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKYSF 266
            DA EFKP+RF +G++KA +  N   + PF  G R C+G NFA+ EAK+AM++IL+K+SF
Sbjct: 428 NDAGEFKPDRFKDGLSKATK--NQVSFFPFAWGPRICIGQNFALLEAKMAMTLILRKFSF 487

Query: 267 NLSPAYAHTPAQFLTTAPQRGLQLILHSV 296
            LSP+Y H P   LTT PQ G  LILH +
Sbjct: 488 ELSPSYVHAPYTVLTTHPQFGAPLILHKL 512

[Close]

BLAST of Lag0040189 vs. TAIR 10
Match: AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )

HSP 1 Score: 236.5 bits (602), Expect = 2.8e-62
Identity = 122/269 (45.35%), Postives = 172/269 (63.94%), Query Frame = 0


Query: 27  KIFKSKDDVEGERLEKRMKDCFMEIIKAREEKSMNGEADGYGNDFLGLLVKAKNHSDKSQ 86
           +   +K+++   ++ K +K    EII  R+     GEA    ND LG+L+++ N  D   
Sbjct: 252 RFLPTKNNLRMRQINKEVKSRLREIIMKRQRGMDTGEAP--KNDLLGILLES-NSGDHG- 311

Query: 87  RISVEDIVDECKTFYLAGQETTTVLIAWIMFLLAMHKEWQHEARNEVLKIFGDQN-PTSE 146
            +S+ED+V+EC+ F+ AGQETT VL+ W M +L+ H++WQ +AR E+LK+ G  N P  +
Sbjct: 312 -MSIEDVVEECRLFHFAGQETTAVLLVWTMIMLSHHQKWQDQAREEILKVIGKNNKPNFD 371

Query: 147 GLPKLTTMTMIINECLRLYPPAMTVSRQVAKEVRLGR-LVLPPTLQLTIPTIAVHHDKAF 206
            L +L TM+MI+NE LRLYPP + + R V KE +LG  + LP   Q+ IP + VH D   
Sbjct: 372 ALSRLKTMSMILNEVLRLYPPGILLGRTVEKETKLGEDMTLPGGAQVVIPVLMVHRDPEL 431

Query: 207 WGEDADEFKPERFSEGVAKAMEEANSAGYLPFGLGARNCVGMNFAMNEAKIAMSMILQKY 266
           WGED  EF PERF++G++KA +  N   +LPFG G R C G NFA+ EAK+A+ +ILQ++
Sbjct: 432 WGEDVHEFNPERFADGISKATK--NQVSFLPFGWGPRFCPGQNFALMEAKMALVLILQRF 491

Query: 267 SFNLSPAYAHTPAQFLTTAPQRGLQLILH 294
           SF LSP+Y H P   LT  PQ G  LI H
Sbjct: 492 SFELSPSYTHAPHTVLTLHPQFGAPLIFH 513

The following BLAST results are available for this feature:

BLAST of Lag0040189 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022945316.1	7.0e-124	83.27	cytochrome P450 CYP749A22-like [Cucurbita moschata]	[more]
XP_023543007.1	5.0e-122	82.18	cytochrome P450 CYP749A22-like [Cucurbita pepo subsp. pepo]	[more]
XP_022149052.1	8.5e-122	79.64	cytochrome P450 CYP749A22-like [Momordica charantia] >XP_022149053.1 cytochrome ...	[more]
XP_038891307.1	3.0e-119	79.64	cytochrome P450 CYP749A22-like [Benincasa hispida]	[more]
XP_038892924.1	8.8e-119	77.78	cytochrome P450 CYP749A22-like isoform X1 [Benincasa hispida]	[more]

BLAST of Lag0040189 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
H2DH17	4.6e-86	55.60	Cytochrome P450 CYP749A22 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
H1A981	3.1e-66	44.37	11-oxo-beta-amyrin 30-oxidase OS=Medicago truncatula OX=3880 GN=CYP72A63 PE=1 SV...	[more]
H1A988	1.2e-65	45.83	11-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP72A154 PE=...	[more]
Q9LUC6	1.7e-64	46.85	Cytochrome P450 72A14 OS=Arabidopsis thaliana OX=3702 GN=CYP72A14 PE=2 SV=1	[more]
Q9LUC8	5.5e-63	44.24	Cytochrome P450 72A13 OS=Arabidopsis thaliana OX=3702 GN=CYP72A13 PE=2 SV=1	[more]

BLAST of Lag0040189 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G0I3	3.4e-124	83.27	cytochrome P450 CYP749A22-like OS=Cucurbita moschata OX=3662 GN=LOC111449593 PE=...	[more]
A0A6J1D6R8	4.1e-122	79.64	cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017564 PE...	[more]
A0A1S3BEM5	1.3e-112	75.54	cytochrome P450 CYP749A22-like OS=Cucumis melo OX=3656 GN=LOC103489194 PE=3 SV=1	[more]
A0A1S3BF46	1.3e-112	73.55	cytochrome P450 CYP749A22-like OS=Cucumis melo OX=3656 GN=LOC103489195 PE=3 SV=1	[more]
A0A6J1IKI4	5.1e-112	72.63	cytochrome P450 CYP749A22-like OS=Cucurbita maxima OX=3661 GN=LOC111474764 PE=3 ...	[more]

BLAST of Lag0040189 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14630.1	2.4e-66	45.32	cytochrome P450, family 72, subfamily A, polypeptide 9	[more]
AT3G14610.1	1.2e-65	45.39	cytochrome P450, family 72, subfamily A, polypeptide 7	[more]
AT3G14680.1	1.2e-65	46.85	cytochrome P450, family 72, subfamily A, polypeptide 14	[more]
AT3G14660.1	3.9e-64	44.24	cytochrome P450, family 72, subfamily A, polypeptide 13	[more]
AT3G14620.1	2.8e-62	45.35	cytochrome P450, family 72, subfamily A, polypeptide 8	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 243..254 score: 39.8 coord: 103..120 score: 36.1 coord: 155..166 score: 41.95 coord: 234..243 score: 59.91
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 28..272 e-value: 7.3E-57 score: 193.1
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 92..109 score: 26.68 coord: 195..219 score: 23.11 coord: 112..138 score: 21.85 coord: 243..266 score: 28.2 coord: 233..243 score: 53.27
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 19..296 e-value: 1.9E-76 score: 259.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 39..292
None	No IPR available	PANTHER	PTHR24282:SF20	CYTOCHROME P450 CYP749A22-LIKE	coord: 27..291
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 27..291
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 236..245

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0040189.1	Lag0040189.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen