Lag0040164 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040164
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionMethyltransferase
Locationchr13: 2611182 .. 2614828 (+)
RNA-Seq ExpressionLag0040164
SyntenyLag0040164
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTTATGCTCTGCTTCAAGATTTAAACCGAAGGCGCTTCCTATTTAGAGTGGTTCTGAACTGGATTATTGAAGGGACTTCAGAAATATTGGACATGAGAAGCTCCTGGGTCAATAAAATATCTGTAATCTTAGGATCCAAACCACCATTCAGCTGGTTAATTTTGTGCCTCATCAGCGTGCTTGCGTTAATTGCAGTGCTAGGAACGTCTACTTCAAATGCCTTTGATTCAGTTACTACCACGCCAGTGTCTGACATTTATACTAGTTATAGAAGGCAAAAAGAAAGGGCCGCAAATGATTTGTTCGACTTAAAGTATCTTTCATTAGCTACAACTAGACTCAAAGAGTTTGGGCTTTGTGGAAAAGAAAGAGAAAACCACGTGCCATGTTACAATGTTACAGCAAACATGTTATCTGGATACAAAGAAGGGGAGGAGTATGATAGGCACTGTGAGGTGTCTAGAACAACAGAGCGGTGCCTGGTTCGTCCTCCTAAAGACTACAAGATCCCTCTGAGTTGGCCTGTTGGTAGGGATATAATATGGAGTGGAAATGTGAAGGTAACAAGAGATCAACTTCTGTCTTCTGGGAGCCCTACCAAAAGGTACCCAACCTCAACTGGACGAGCATGAATTAAGCACTTAGATTTTTTTCCCCTAAGGATTGAGTATTTAGAACCAAAACAGTTGTTTTCTTTTTGTTTCTGTGGTCATTTTTTATTGATTAATATAACCATCTTACTTTTCCAAATAAATTGATCAGGTTGATGTTATTAGAAGAGAACCAAATTGCTTTCCACTCGGAGGATGGAGATGGAGTCAAAGAGTATTCTTTTCAAATCGCAGAGATGATTGGTTTAGGCTCCGACTCTGAATTTCTTCAAGCTGGTGTAAGTCTTAATCTCATGTAAGAAATATTATAGGCTTTTGTCTTCTTTTGTTATGGTAATTTTGTGTGTTTTTAATGCCAGCTACATCATGGAGTGAATCAAGTTAAATGTTTTTTCTTTAAAATTACCCAAACAATAAACTTATATTAAGTAGTAAATAGTAACCCATGTTTCTGTCCTTTCAATCTTTTTTGCAATTCAGTGCAGTAAACCTATTTAATGTATCAAGGATTTGATCATATGTCAATTATAGGTACGCTCCATACTTGATATTGGTTGTGGGTTTGGTAGCTTTGGTGCTCATTTAATATCAATGAAAGTAATGGTTATGTGCATTGCGACCTATGAAGCGACTGGCAGCCAAGTTCAAATGGCTCTCGAGAGAGGTCTTCCAGCGATGCTTGGAAGCTTTCTTACAAAACAGCTTCCATATCCATCCTTGTCATTTGACATGGTTCACTGTGCACAGTGTGGTATCTCTTGGAACGATAAAGGTAACTTGAGAATTAAATTTAGATGCATCTTATTTTATTTGATGGGTATATCATAAAAGCCTAGATGGGTTTGGTTGGGTTAGAGACACTTGGTACAAGTTTTTAAGATTAAGAAAGAAATAGGATGGACATTTCTTTCTATAGATATGCCAAAACAATATGGTGCAGTTAGAAACCACAAACAACTATATGTATGCATATTAGTGCATATTATGTTCTTGAATCTTCATGAGATTTTTTCAGTATCAGTGTAGTTTAATTGATTTGATATTTTCTCTTGTTTTCTTGCTTCATGAATAATGCGCAGGTGGAATATTCCTTATTGAAGCTGACCGGTTACTAAGACCTGGAGGTTATTTTGTTTTAACCTCTCCCACAGGCAAGCCAGTTGGAGGTTCATTGTCTTCCAAGAAGATGAACATTTTGACACCATTGGAAGAGATGACCAGAAAATTATGTTGGAATTTTTTGGCTCAGCAGTATGAAACTTACATTTGGCAGAAAACTTCAGATCCTCATTGCTATTTATCTCGGTGAGCACAATACTTATGTTCCTCCTAATTAACCTTCGTGTTCATTTTTGGTTGTGTTGTTTTTTGAACTTCAGTCAGAACCTTCTCTACTGCATCGTACAGTGTACCATTTGTTTGATTGTGCTTGTAGCTTTGCTGTCGTTGGTAATTGTTGTGTAAAAACTTAGTAATGCTTCGGCTTCTTTCTTCTTTTAGCAAGCAGGAAGTTGTACCACTTTGTAAAGAAGGGCATGATACTCCATCATATTATCAACCTCTGGTACCATGCATAAGTAGTACTACCAGCAAGCGGTGGATTCCAATCCAGAACAGGTCTTCTGGTTCCCATTTGAGTTCAGCTGAACTTGAAGTTCATGGAAAGTATTCTTCCATCATGGTTCTATATTGTTTTTTCATGTTAATTTGTTGTTTATTCCAATTGTTGTTATTATTATTATTATTTATCATGAAACAACTCCATCTTTGACTGAAATCCATGGACTTTTTTTTTGGTGTTGTGACTGAACCATTTTCATCTTCTGTTCTTTTTGTGTTATTTTGATCTGTACTGTGTGCACAGAAACTTATCGTGCACTTTGTGCTGCTATTGATTACTTTCCTGTGTCTATATCTGCAGGCGTTCGATCAGACGACTACTCTGATGAATTACAAATTTGGCGATCTGCTTTGAAAAACTATTGGTCCTTACTTACGCCTTTAATTTTCTCTGACCATCCAAAGAGGCCTGGTGATGAAGATCCATTACCTCCATTTAACATGATACGCAATGTCATGGACATGAACGCCCACTATGGGGGTTTAAACGCTGCGTTTGTGGAAGAGAAGAAAACAGTTTGGGTCATGAATGTTGTGCCTGTTGGGTCTCCTAATACGCTTCCGCTTATACTTGATCAAGGTTTTGCTGGTGTTCTGCACGATTGGTATGCAGTTCTTACTTCCAGTCTTCATAAAAAAATTTTCCTTCTAAGAGCAGACTTTGTACTTCTCGGTCCAATTGGTTTGTGATGAGTTGGAATTGGAAACATCCTGGCCATACTAACCTTTTTCTTAAAGAAACAAATATTCAACTTTTTATTGATGATTGCATATTGTGCAAGGCTTTCTGTAATGGAATGTCACTGTTGTTTCTTGCTTTTTTTTTTTAATTTCTCTTTTAATTTTCATTATTTAAAGTCAAGAAGCAACAAGTATCTGAATCTTTGACACGTCTAAATAATAATATTTCAAATTTTTTGAGTACCAGTGCCACATTATGTACTTGTTTCCAGTCAGTTTTAGTCTGGTTCTCCCCCCCCCCCCCAATGTACGGGCACTTTGAACTAGACTTAATTTCCCATCTACTTGTAGGTGTGAACCTTTCCCCACTTATCCTCGAACATATGATTTGCTCCATGCAAATGGGCTCCTCTCACAGCTACTTTCAAGTAGGTGCAGCATGATTGGATTATTAGTGGAGATGGATCGTATACTGCGTCCTGAGGTACTAGTCCGTCATAAAAAATTAAGTCAGGGCTTCTTTTTCTCTCTGGTGCAGCCGTGCAAATATTATATTGATCATTTTGACTGTTGTTTGTCAGGGATGGGTTGTCCTAAAAGACAAAGTGGGACCGATAGAGAAGGCGCGAATGCTTGCTACACAGATTCGTTGGGAGGCAAGGGTGATTGACTCTCAGAATGGCAGTGACCAGAGGCTCCTTGTTTGCCAGAAACCATTCGTAAAAAAATGA

mRNA sequence

ATGCTTTATGCTCTGCTTCAAGATTTAAACCGAAGGCGCTTCCTATTTAGAGTGGTTCTGAACTGGATTATTGAAGGGACTTCAGAAATATTGGACATGAGAAGCTCCTGGGTCAATAAAATATCTGTAATCTTAGGATCCAAACCACCATTCAGCTGGTTAATTTTGTGCCTCATCAGCGTGCTTGCGTTAATTGCAGTGCTAGGAACGTCTACTTCAAATGCCTTTGATTCAGTTACTACCACGCCAGTGTCTGACATTTATACTAGTTATAGAAGGCAAAAAGAAAGGGCCGCAAATGATTTGTTCGACTTAAAGTATCTTTCATTAGCTACAACTAGACTCAAAGAGTTTGGGCTTTGTGGAAAAGAAAGAGAAAACCACGTGCCATGTTACAATGTTACAGCAAACATGTTATCTGGATACAAAGAAGGGGAGGAGTATGATAGGCACTGTGAGGTGTCTAGAACAACAGAGCGGTGCCTGGTTCGTCCTCCTAAAGACTACAAGATCCCTCTGAGTTGGCCTGTTGGTAGGGATATAATATGGAGTGGAAATGTGAAGGTAACAAGAGATCAACTTCTGTCTTCTGGGAGCCCTACCAAAAGGTTGATGTTATTAGAAGAGAACCAAATTGCTTTCCACTCGGAGGATGGAGATGGAGTCAAAGAGTATTCTTTTCAAATCGCAGAGATGATTGGTTTAGGCTCCGACTCTGAATTTCTTCAAGCTGGTGTACGCTCCATACTTGATATTGGTTGTGGGTTTGGTAGCTTTGGTGCTCATTTAATATCAATGAAAGTAATGGTTATGTGCATTGCGACCTATGAAGCGACTGGCAGCCAAGTTCAAATGGCTCTCGAGAGAGGTCTTCCAGCGATGCTTGGAAGCTTTCTTACAAAACAGCTTCCATATCCATCCTTGTCATTTGACATGGTTCACTGTGCACAGTGTGGTATCTCTTGGAACGATAAAGGTGGAATATTCCTTATTGAAGCTGACCGGTTACTAAGACCTGGAGGTTATTTTGTTTTAACCTCTCCCACAGGCAAGCCAGTTGGAGGTTCATTGTCTTCCAAGAAGATGAACATTTTGACACCATTGGAAGAGATGACCAGAAAATTATGTTGGAATTTTTTGGCTCAGCAGTATGAAACTTACATTTGGCAGAAAACTTCAGATCCTCATTGCTATTTATCTCGCAAGCAGGAAGTTGTACCACTTTGTAAAGAAGGGCATGATACTCCATCATATTATCAACCTCTGGTACCATGCATAAGTAGTACTACCAGCGTTCGATCAGACGACTACTCTGATGAATTACAAATTTGGCGATCTGCTTTGAAAAACTATTGGTCCTTACTTACGCCTTTAATTTTCTCTGACCATCCAAAGAGGCCTGGTGATGAAGATCCATTACCTCCATTTAACATGATACGCAATGTCATGGACATGAACGCCCACTATGGGGGTTTAAACGCTGCGTTTGTGGAAGAGAAGAAAACAGTTTGGGTCATGAATGTTGTGCCTGTTGGGTCTCCTAATACGCTTCCGCTTATACTTGATCAAGGTTTTGCTGGTGTTCTGCACGATTGGTGTGAACCTTTCCCCACTTATCCTCGAACATATGATTTGCTCCATGCAAATGGGCTCCTCTCACAGCTACTTTCAAGTAGGTGCAGCATGATTGGATTATTAGTGGAGATGGATCGTATACTGCGTCCTGAGGGATGGGTTGTCCTAAAAGACAAAGTGGGACCGATAGAGAAGGCGCGAATGCTTGCTACACAGATTCGTTGGGAGGCAAGGGTGATTGACTCTCAGAATGGCAGTGACCAGAGGCTCCTTGTTTGCCAGAAACCATTCGTAAAAAAATGA

Coding sequence (CDS)

ATGCTTTATGCTCTGCTTCAAGATTTAAACCGAAGGCGCTTCCTATTTAGAGTGGTTCTGAACTGGATTATTGAAGGGACTTCAGAAATATTGGACATGAGAAGCTCCTGGGTCAATAAAATATCTGTAATCTTAGGATCCAAACCACCATTCAGCTGGTTAATTTTGTGCCTCATCAGCGTGCTTGCGTTAATTGCAGTGCTAGGAACGTCTACTTCAAATGCCTTTGATTCAGTTACTACCACGCCAGTGTCTGACATTTATACTAGTTATAGAAGGCAAAAAGAAAGGGCCGCAAATGATTTGTTCGACTTAAAGTATCTTTCATTAGCTACAACTAGACTCAAAGAGTTTGGGCTTTGTGGAAAAGAAAGAGAAAACCACGTGCCATGTTACAATGTTACAGCAAACATGTTATCTGGATACAAAGAAGGGGAGGAGTATGATAGGCACTGTGAGGTGTCTAGAACAACAGAGCGGTGCCTGGTTCGTCCTCCTAAAGACTACAAGATCCCTCTGAGTTGGCCTGTTGGTAGGGATATAATATGGAGTGGAAATGTGAAGGTAACAAGAGATCAACTTCTGTCTTCTGGGAGCCCTACCAAAAGGTTGATGTTATTAGAAGAGAACCAAATTGCTTTCCACTCGGAGGATGGAGATGGAGTCAAAGAGTATTCTTTTCAAATCGCAGAGATGATTGGTTTAGGCTCCGACTCTGAATTTCTTCAAGCTGGTGTACGCTCCATACTTGATATTGGTTGTGGGTTTGGTAGCTTTGGTGCTCATTTAATATCAATGAAAGTAATGGTTATGTGCATTGCGACCTATGAAGCGACTGGCAGCCAAGTTCAAATGGCTCTCGAGAGAGGTCTTCCAGCGATGCTTGGAAGCTTTCTTACAAAACAGCTTCCATATCCATCCTTGTCATTTGACATGGTTCACTGTGCACAGTGTGGTATCTCTTGGAACGATAAAGGTGGAATATTCCTTATTGAAGCTGACCGGTTACTAAGACCTGGAGGTTATTTTGTTTTAACCTCTCCCACAGGCAAGCCAGTTGGAGGTTCATTGTCTTCCAAGAAGATGAACATTTTGACACCATTGGAAGAGATGACCAGAAAATTATGTTGGAATTTTTTGGCTCAGCAGTATGAAACTTACATTTGGCAGAAAACTTCAGATCCTCATTGCTATTTATCTCGCAAGCAGGAAGTTGTACCACTTTGTAAAGAAGGGCATGATACTCCATCATATTATCAACCTCTGGTACCATGCATAAGTAGTACTACCAGCGTTCGATCAGACGACTACTCTGATGAATTACAAATTTGGCGATCTGCTTTGAAAAACTATTGGTCCTTACTTACGCCTTTAATTTTCTCTGACCATCCAAAGAGGCCTGGTGATGAAGATCCATTACCTCCATTTAACATGATACGCAATGTCATGGACATGAACGCCCACTATGGGGGTTTAAACGCTGCGTTTGTGGAAGAGAAGAAAACAGTTTGGGTCATGAATGTTGTGCCTGTTGGGTCTCCTAATACGCTTCCGCTTATACTTGATCAAGGTTTTGCTGGTGTTCTGCACGATTGGTGTGAACCTTTCCCCACTTATCCTCGAACATATGATTTGCTCCATGCAAATGGGCTCCTCTCACAGCTACTTTCAAGTAGGTGCAGCATGATTGGATTATTAGTGGAGATGGATCGTATACTGCGTCCTGAGGGATGGGTTGTCCTAAAAGACAAAGTGGGACCGATAGAGAAGGCGCGAATGCTTGCTACACAGATTCGTTGGGAGGCAAGGGTGATTGACTCTCAGAATGGCAGTGACCAGAGGCTCCTTGTTTGCCAGAAACCATTCGTAAAAAAATGA

Protein sequence

MLYALLQDLNRRRFLFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFSWLILCLISVLALIAVLGTSTSNAFDSVTTTPVSDIYTSYRRQKERAANDLFDLKYLSLATTRLKEFGLCGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTTERCLVRPPKDYKIPLSWPVGRDIIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSEFLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQVQMALERGLPAMLGSFLTKQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKPVGGSLSSKKMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQPLVPCISSTTSVRSDDYSDELQIWRSALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNVVPVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEMDRILRPEGWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLVCQKPFVKK
Homology
BLAST of Lag0040164 vs. NCBI nr
Match: XP_038892405.1 (probable methyltransferase PMT5 [Benincasa hispida] >XP_038892406.1 probable methyltransferase PMT5 [Benincasa hispida] >XP_038892407.1 probable methyltransferase PMT5 [Benincasa hispida])

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 595/649 (91.68%), Postives = 607/649 (93.53%), Query Frame = 0

Query: 1   MLYALLQDLNRRRFLFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60
           MLYALL+DLNRR F FRVVLNWII+GTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS
Sbjct: 1   MLYALLRDLNRRHFPFRVVLNWIIKGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60

Query: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYTSYRRQKERAANDLFDLKYLSLATTRLKEFGL 120
           VLALIAVLGTSTSNAFDSVTTTPVSDIY SYRRQKERAA DLFDLK LSLATTRLKEFGL
Sbjct: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYASYRRQKERAAIDLFDLKSLSLATTRLKEFGL 120

Query: 121 CGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTTERCLVRPPKDYKIPLSWPVGRD 180
           CGKERENHVPCYNVTANML+GYKEGEEYDRHCEVSRT  RCLVRPPKDYKIPLSWPVGRD
Sbjct: 121 CGKERENHVPCYNVTANMLAGYKEGEEYDRHCEVSRTAGRCLVRPPKDYKIPLSWPVGRD 180

Query: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240
           IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE
Sbjct: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240

Query: 241 FLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQVQMALERGLPAMLGSFLT 300
           FLQAGVRSILDIGCGFGSFGAHLISM VMVMCIATYEATGSQVQMALERGLPAMLG+F+ 
Sbjct: 241 FLQAGVRSILDIGCGFGSFGAHLISMNVMVMCIATYEATGSQVQMALERGLPAMLGNFIR 300

Query: 301 KQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKPVGGSLSSK 360
           KQLPYPSLSFDMVHCAQC ISWNDKGGIFLIEADRLLRPGGYFVLTSPTGK VGGSLSSK
Sbjct: 301 KQLPYPSLSFDMVHCAQCDISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKTVGGSLSSK 360

Query: 361 KMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQ 420
           K NILTPLEEMTRKLCW+ LAQQYETYIWQKT+DPHCYLS KQEVVP+CKEGHDTPSYYQ
Sbjct: 361 KPNILTPLEEMTRKLCWSLLAQQYETYIWQKTTDPHCYLSGKQEVVPVCKEGHDTPSYYQ 420

Query: 421 PLVPCISSTTS------------------------VRSDDYSDELQIWRSALKNYWSLLT 480
           PLVPCISSTTS                        V+ +DYSDELQIW+SALKNYWSLLT
Sbjct: 421 PLVPCISSTTSKRWIPIQNRSSGSHLNSAELEVHGVQLEDYSDELQIWQSALKNYWSLLT 480

Query: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNVVPVGSPNT 540
           PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVE+KKTVWVMNVVPVGSPNT
Sbjct: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEQKKTVWVMNVVPVGSPNT 540

Query: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEMDRILRPE 600
           LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLL SRCSMIGLLVEMDRILRPE
Sbjct: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLPSRCSMIGLLVEMDRILRPE 600

Query: 601 GWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLVCQKPFVKK 626
           GWVVLKDKVGPIEKARM ATQIRWEARVID QNGSDQRLLVCQKPFVKK
Sbjct: 601 GWVVLKDKVGPIEKARMFATQIRWEARVIDFQNGSDQRLLVCQKPFVKK 649

BLAST of Lag0040164 vs. NCBI nr
Match: XP_008446426.1 (PREDICTED: probable methyltransferase PMT5 [Cucumis melo] >XP_008446427.1 PREDICTED: probable methyltransferase PMT5 [Cucumis melo])

HSP 1 Score: 1210.7 bits (3131), Expect = 0.0e+00
Identity = 590/649 (90.91%), Postives = 605/649 (93.22%), Query Frame = 0

Query: 1   MLYALLQDLNRRRFLFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60
           MLYAL +DLNRRRF FRVVLNWII+GTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS
Sbjct: 1   MLYALFRDLNRRRFPFRVVLNWIIKGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60

Query: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYTSYRRQKERAANDLFDLKYLSLATTRLKEFGL 120
           VLALIAVLGTSTSNAFDSVTTTPVSDIY SYRRQKERAA DLFDLK LSLATTRLKEFGL
Sbjct: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYASYRRQKERAAIDLFDLKSLSLATTRLKEFGL 120

Query: 121 CGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTTERCLVRPPKDYKIPLSWPVGRD 180
           CGKERENHVPCYNVTAN+L+GYKEGEEYDRHCEVSRT ERCLVRPPKDYKIPLSWPVGRD
Sbjct: 121 CGKERENHVPCYNVTANLLAGYKEGEEYDRHCEVSRTAERCLVRPPKDYKIPLSWPVGRD 180

Query: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240
           IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE
Sbjct: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240

Query: 241 FLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQVQMALERGLPAMLGSFLT 300
           F QAGVRSILDIGCGFGS GAHLIS+ VM MCIATYEATGSQVQMALERGLPAMLG+F T
Sbjct: 241 FFQAGVRSILDIGCGFGSLGAHLISLNVMAMCIATYEATGSQVQMALERGLPAMLGNFAT 300

Query: 301 KQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKPVGGSLSSK 360
           KQLPYPSLSFDMVHCAQC ISWNDKGGIFLIEADRLLRPGGYFVLTSPTGK +GGSLSSK
Sbjct: 301 KQLPYPSLSFDMVHCAQCDISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKTIGGSLSSK 360

Query: 361 KMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQ 420
           K NILTPLEEMT+KLCW  LAQQYETYIWQKT+DPHCY SRKQEVVPLCKE HDTPSYYQ
Sbjct: 361 KTNILTPLEEMTKKLCWILLAQQYETYIWQKTTDPHCYSSRKQEVVPLCKEAHDTPSYYQ 420

Query: 421 PLVPCISSTTS------------------------VRSDDYSDELQIWRSALKNYWSLLT 480
           PLVPC+SSTTS                        V+S+DYSDELQIWRSALKNYWSLLT
Sbjct: 421 PLVPCLSSTTSKRWIPIQNRSSGSHLSSAELEVHGVQSEDYSDELQIWRSALKNYWSLLT 480

Query: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNVVPVGSPNT 540
           PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVE+KKTVWVMNVVPVGSPNT
Sbjct: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEQKKTVWVMNVVPVGSPNT 540

Query: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEMDRILRPE 600
           LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRC+MIGLL+EMDRILRPE
Sbjct: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCNMIGLLLEMDRILRPE 600

Query: 601 GWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLVCQKPFVKK 626
           GWVVLKDKVGPIEK RMLATQIRWEARVID QNGSDQRLLVCQKPFVKK
Sbjct: 601 GWVVLKDKVGPIEKVRMLATQIRWEARVIDFQNGSDQRLLVCQKPFVKK 649

BLAST of Lag0040164 vs. NCBI nr
Match: XP_031740879.1 (probable methyltransferase PMT5 [Cucumis sativus])

HSP 1 Score: 1208.7 bits (3126), Expect = 0.0e+00
Identity = 588/649 (90.60%), Postives = 606/649 (93.37%), Query Frame = 0

Query: 1   MLYALLQDLNRRRFLFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60
           MLYAL +DLN+RRF FRVVL+WI++GTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS
Sbjct: 1   MLYALFRDLNQRRFPFRVVLHWIVKGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60

Query: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYTSYRRQKERAANDLFDLKYLSLATTRLKEFGL 120
           VLALIAVLGTSTSNAFDSVTTTPVSDIY SYRRQKERAA DLFDLK LSLATTRLKEFGL
Sbjct: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYASYRRQKERAAIDLFDLKSLSLATTRLKEFGL 120

Query: 121 CGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTTERCLVRPPKDYKIPLSWPVGRD 180
           CGKERENHVPCYNVTAN+L+GYKEGEEYDRHCEVSRT +RCLVRPPKDYKIPLSWPVGRD
Sbjct: 121 CGKERENHVPCYNVTANLLAGYKEGEEYDRHCEVSRTAQRCLVRPPKDYKIPLSWPVGRD 180

Query: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240
           IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE
Sbjct: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240

Query: 241 FLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQVQMALERGLPAMLGSFLT 300
           F QAGVRSILDIGCGFGS GAHLIS+ VMVMCIATYEATGSQVQMALERGLPAMLG+F+T
Sbjct: 241 FFQAGVRSILDIGCGFGSLGAHLISLNVMVMCIATYEATGSQVQMALERGLPAMLGNFVT 300

Query: 301 KQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKPVGGSLSSK 360
           KQLPYPSLSFDMVHCAQC ISWNDKGGIFLIEADRLLRPGGYFVLTSPTGK +GGSLSSK
Sbjct: 301 KQLPYPSLSFDMVHCAQCDISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKTIGGSLSSK 360

Query: 361 KMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQ 420
           K NILTPLEEMT+KLCW  LAQQYETYIWQKT+DPHCY SRKQEVVPLCKE HDTPSYYQ
Sbjct: 361 KTNILTPLEEMTKKLCWILLAQQYETYIWQKTTDPHCYFSRKQEVVPLCKEAHDTPSYYQ 420

Query: 421 PLVPCISSTTS------------------------VRSDDYSDELQIWRSALKNYWSLLT 480
           PLVPCISSTTS                        V+S+DYSDELQIW+SALKNYWSLLT
Sbjct: 421 PLVPCISSTTSKRWIPIYNRSSGSHLSSAELEVHGVQSEDYSDELQIWQSALKNYWSLLT 480

Query: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNVVPVGSPNT 540
           PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVE+KKTVWVMNVVPVGSPNT
Sbjct: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEQKKTVWVMNVVPVGSPNT 540

Query: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEMDRILRPE 600
           LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEMDRILRPE
Sbjct: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEMDRILRPE 600

Query: 601 GWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLVCQKPFVKK 626
           GWVV KDKVGPIEK RMLATQIRWEARVID QNGSDQRLLVCQKPFVKK
Sbjct: 601 GWVVFKDKVGPIEKVRMLATQIRWEARVIDFQNGSDQRLLVCQKPFVKK 649

BLAST of Lag0040164 vs. NCBI nr
Match: XP_023541770.1 (probable methyltransferase PMT5 [Cucurbita pepo subsp. pepo] >XP_023541771.1 probable methyltransferase PMT5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1197.2 bits (3096), Expect = 0.0e+00
Identity = 580/649 (89.37%), Postives = 604/649 (93.07%), Query Frame = 0

Query: 1   MLYALLQDLNRRRFLFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60
           MLYALL+DLNRRRF FRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPF+WLILCLIS
Sbjct: 1   MLYALLRDLNRRRFPFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFNWLILCLIS 60

Query: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYTSYRRQKERAANDLFDLKYLSLATTRLKEFGL 120
           VLALI VLGTSTSN FDSV+TTPVSDIYTSYRRQKERAA DLFDLK LSLAT RLKEFGL
Sbjct: 61  VLALIVVLGTSTSNVFDSVSTTPVSDIYTSYRRQKERAAIDLFDLKSLSLATGRLKEFGL 120

Query: 121 CGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTTERCLVRPPKDYKIPLSWPVGRD 180
           CGKERENHVPCYNVTANML+GYKEGEEYDRHCEVSRT ERCLVRPPKDYKIPLSWPVGRD
Sbjct: 121 CGKERENHVPCYNVTANMLAGYKEGEEYDRHCEVSRTAERCLVRPPKDYKIPLSWPVGRD 180

Query: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240
           IIWSGN+KVT+DQLLSSGSPTKRLMLLEENQI+FHSEDGDGVKEYSFQIAEMIGLG+DSE
Sbjct: 181 IIWSGNLKVTKDQLLSSGSPTKRLMLLEENQISFHSEDGDGVKEYSFQIAEMIGLGTDSE 240

Query: 241 FLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQVQMALERGLPAMLGSFLT 300
           FLQAGVRSILDIGCGFGSFGAHL SM VMVMCIATYEATGSQVQMALERGLPAMLG+F+T
Sbjct: 241 FLQAGVRSILDIGCGFGSFGAHLTSMNVMVMCIATYEATGSQVQMALERGLPAMLGNFIT 300

Query: 301 KQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKPVGGSLSSK 360
           KQLPYPSLSFDMVHCAQC ISWNDKGGIFLIEADRLLRPGGYFVLTSPT K VGGSLSSK
Sbjct: 301 KQLPYPSLSFDMVHCAQCEISWNDKGGIFLIEADRLLRPGGYFVLTSPTSKAVGGSLSSK 360

Query: 361 KMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQ 420
           K++ILTPLEE+TR+LCW FLAQQYETYIWQKT+DP CYLS KQE+VPLCKEGHDTPSYYQ
Sbjct: 361 KISILTPLEEITRRLCWTFLAQQYETYIWQKTTDPRCYLSGKQEIVPLCKEGHDTPSYYQ 420

Query: 421 PLVPCISSTTS------------------------VRSDDYSDELQIWRSALKNYWSLLT 480
           PLVPC+SSTTS                        V+SDDY+D+LQIWR+ALKNYWSLLT
Sbjct: 421 PLVPCLSSTTSKRWIPIQNRSSSFRLSSAELEVHGVQSDDYADDLQIWRTALKNYWSLLT 480

Query: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNVVPVGSPNT 540
           PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVE+KKTVWVMNVVPVGSPNT
Sbjct: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEQKKTVWVMNVVPVGSPNT 540

Query: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEMDRILRPE 600
           LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSM+  LVEMDRILRPE
Sbjct: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMLEFLVEMDRILRPE 600

Query: 601 GWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLVCQKPFVKK 626
           GWV+L DKVGPIEKARML T IRWEARVID QNGSDQRLLVCQKPFVKK
Sbjct: 601 GWVILNDKVGPIEKARMLVTHIRWEARVIDFQNGSDQRLLVCQKPFVKK 649

BLAST of Lag0040164 vs. NCBI nr
Match: XP_022945429.1 (probable methyltransferase PMT5 [Cucurbita moschata] >XP_022945430.1 probable methyltransferase PMT5 [Cucurbita moschata])

HSP 1 Score: 1194.9 bits (3090), Expect = 0.0e+00
Identity = 579/649 (89.21%), Postives = 603/649 (92.91%), Query Frame = 0

Query: 1   MLYALLQDLNRRRFLFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60
           MLYALL+DLNRRRF FRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPF+WLILCLIS
Sbjct: 1   MLYALLRDLNRRRFPFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFNWLILCLIS 60

Query: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYTSYRRQKERAANDLFDLKYLSLATTRLKEFGL 120
           VLALI VLGTSTSN FDSV+TTPVSDIYTSYRRQKERAA DLFDLK LSLAT RLKEFGL
Sbjct: 61  VLALIVVLGTSTSNVFDSVSTTPVSDIYTSYRRQKERAAIDLFDLKSLSLATGRLKEFGL 120

Query: 121 CGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTTERCLVRPPKDYKIPLSWPVGRD 180
           CGKERENHVPCYNVTAN L+GYKEGEEYDRHCEVSRT ERCLVRPPKDYKIPLSWPVGRD
Sbjct: 121 CGKERENHVPCYNVTANTLAGYKEGEEYDRHCEVSRTAERCLVRPPKDYKIPLSWPVGRD 180

Query: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240
           IIWSGN+KVT+DQLLSSGSPTKRLMLLEENQI+FHSEDGDGVKEYSFQIAEMIGLG+DSE
Sbjct: 181 IIWSGNLKVTKDQLLSSGSPTKRLMLLEENQISFHSEDGDGVKEYSFQIAEMIGLGTDSE 240

Query: 241 FLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQVQMALERGLPAMLGSFLT 300
           FLQAGVRSILDIGCGFGSFGAHL SM VMVMCIATYEATGSQVQMALERGLPAMLG+F+T
Sbjct: 241 FLQAGVRSILDIGCGFGSFGAHLTSMNVMVMCIATYEATGSQVQMALERGLPAMLGNFIT 300

Query: 301 KQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKPVGGSLSSK 360
           KQLPYPSLSFDMVHCAQC ISWNDKGGIFLIEADRLLRPGGYFVLTSPT K VGGSLSSK
Sbjct: 301 KQLPYPSLSFDMVHCAQCEISWNDKGGIFLIEADRLLRPGGYFVLTSPTSKAVGGSLSSK 360

Query: 361 KMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQ 420
           K++ILTPLEE+TR+LCW FLAQQYETYIWQKT+DP CYLS KQE+VPLCKEGHDTPSYYQ
Sbjct: 361 KISILTPLEEITRRLCWTFLAQQYETYIWQKTTDPRCYLSGKQEIVPLCKEGHDTPSYYQ 420

Query: 421 PLVPCISSTTS------------------------VRSDDYSDELQIWRSALKNYWSLLT 480
           PLVPC+SSTTS                        V+SDDY+D+LQIWR+ALKNYWSLLT
Sbjct: 421 PLVPCLSSTTSKRWIPIQNRSSSSRLSSAELEVHGVQSDDYADDLQIWRTALKNYWSLLT 480

Query: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNVVPVGSPNT 540
           PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVE+KKTVWVMNVVPVGSPNT
Sbjct: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEQKKTVWVMNVVPVGSPNT 540

Query: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEMDRILRPE 600
           LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSM+  LVEMDRILRPE
Sbjct: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMLEFLVEMDRILRPE 600

Query: 601 GWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLVCQKPFVKK 626
           GWV+L DKVGPIEKARML T IRWEARVID QNGSDQRLLVCQKPFVKK
Sbjct: 601 GWVILNDKVGPIEKARMLVTHIRWEARVIDFQNGSDQRLLVCQKPFVKK 649

BLAST of Lag0040164 vs. ExPASy Swiss-Prot
Match: Q3EC77 (Probable methyltransferase PMT5 OS=Arabidopsis thaliana OX=3702 GN=At2g03480 PE=2 SV=2)

HSP 1 Score: 843.2 bits (2177), Expect = 1.9e-243
Identity = 404/608 (66.45%), Postives = 487/608 (80.10%), Query Frame = 0

Query: 33  MRSSWVNKISVILGSKPPFSWLILCLISVLALIAVLGTSTSNAFD-SVTTTPVSDIYTSY 92
           MR SW   +S + G +P    L+  ++ V+AL+ +L   TSN++D S ++T V +IY++Y
Sbjct: 1   MRGSWYKSVSSVFGLRPRIRGLLFFIVGVVALVTILAPLTSNSYDSSSSSTLVPNIYSNY 60

Query: 93  RRQKERAANDLFDLKYLSLATTRLKEFGLCGKERENHVPCYNVTANMLSGYKEGEEYDRH 152
           RR KE+AA D  DL+ LSL  + LKEF  CGKERE++VPCYN+T N+L+G +EGEE DRH
Sbjct: 61  RRIKEQAAVDYLDLRSLSLGAS-LKEFPFCGKERESYVPCYNITGNLLAGLQEGEELDRH 120

Query: 153 CEVSRTTERCLVRPPKDYKIPLSWPVGRDIIWSGNVKVTRDQLLSSGSPTKRLMLLEENQ 212
           CE  R  ERC+VRPP+DYKIPL WP+GRDIIWSGNVK+T+DQ LSSG+ T RLMLLEENQ
Sbjct: 121 CEFEREKERCVVRPPRDYKIPLRWPLGRDIIWSGNVKITKDQFLSSGTVTTRLMLLEENQ 180

Query: 213 IAFHSEDG---DGVKEYSFQIAEMIGLGSDSEFLQAGVRSILDIGCGFGSFGAHLISMKV 272
           I FHSEDG   DGVK+Y+ QIAEMIGLGSD+EF QAGVR++LDIGCGFGSFGAHL+S+K+
Sbjct: 181 ITFHSEDGLVFDGVKDYARQIAEMIGLGSDTEFAQAGVRTVLDIGCGFGSFGAHLVSLKL 240

Query: 273 MVMCIATYEATGSQVQMALERGLPAMLGSFLTKQLPYPSLSFDMVHCAQCGISWNDKGGI 332
           M +CIA YEATGSQVQ+ALERGLPAM+G+F +KQLPYP+LSFDMVHCAQCG +W+ K  +
Sbjct: 241 MPICIAEYEATGSQVQLALERGLPAMIGNFFSKQLPYPALSFDMVHCAQCGTTWDIKDAM 300

Query: 333 FLIEADRLLRPGGYFVLTSPTGKPVGGSLSSKKMNILTPLEEMTRKLCWNFLAQQYETYI 392
            L+E DR+L+PGGYFVLTSPT K  G    +KK +I T + E+++K+CW+  AQQ ET++
Sbjct: 301 LLLEVDRVLKPGGYFVLTSPTNKAQGNLPDTKKTSISTRVNELSKKICWSLTAQQDETFL 360

Query: 393 WQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQPLVPCISSTTSVR----------SDDYS 452
           WQKTSD  CY SR Q  +PLCK+G   P YY PLVPCIS TTS R          +   S
Sbjct: 361 WQKTSDSSCYSSRSQASIPLCKDGDSVP-YYHPLVPCISGTTSKRWISIQNRSAVAGTTS 420

Query: 453 DELQI-WRSALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFV 512
             L+I  +SALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDM+A +G LNAA +
Sbjct: 421 AGLEIHGKSALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMHARFGNLNAALL 480

Query: 513 EEKKTVWVMNVVPVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLS 572
           +E K+ WVMNVVPV + NTLP+ILD+GFAGVLHDWCEPFPTYPRTYD+LHAN LL+ L S
Sbjct: 481 DEGKSAWVMNVVPVNARNTLPIILDRGFAGVLHDWCEPFPTYPRTYDMLHANELLTHLSS 540

Query: 573 SRCSMIGLLVEMDRILRPEGWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLV 626
            RCS++ L +EMDRILRPEGWVVL DKVG IE AR LA ++RWEARVID Q+GSDQRLLV
Sbjct: 541 ERCSLMDLFLEMDRILRPEGWVVLSDKVGVIEMARALAARVRWEARVIDLQDGSDQRLLV 600

BLAST of Lag0040164 vs. ExPASy Swiss-Prot
Match: Q8GYW9 (Probable methyltransferase PMT4 OS=Arabidopsis thaliana OX=3702 GN=At1g13860 PE=2 SV=2)

HSP 1 Score: 813.5 bits (2100), Expect = 1.6e-234
Identity = 385/614 (62.70%), Postives = 475/614 (77.36%), Query Frame = 0

Query: 40  KISVILGSKPPFSWLILCLISVLALIAVL-GTSTSNAFDSVTTTPVSDIYTSYRRQKERA 99
           K++ ++G +P  S L+   + V+ALI +L   S S++  S T  P S+IY++Y R KE+A
Sbjct: 2   KVASVIGLRPRISGLLFLTLGVIALITILVPNSDSSSTTSTTRVPPSNIYSNYGRVKEQA 61

Query: 100 ANDLFDLKYLSLATTRLKEFGLCGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTT 159
           A D  DL++ SL   RLKEF LCGKER+N+VPCYNVT           E DR+CE +R  
Sbjct: 62  AVDYLDLRFFSLGVNRLKEFPLCGKERDNYVPCYNVT-----------ESDRNCEFAREE 121

Query: 160 ERCLVRPPKDYKIPLSWPVGRDIIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSED 219
           ERCLVRPP+DYKIPL WPVGRDIIW+GNVK+T+DQ LSSG+ TKRLMLLEENQI FHS+D
Sbjct: 122 ERCLVRPPRDYKIPLRWPVGRDIIWTGNVKITKDQFLSSGTMTKRLMLLEENQITFHSDD 181

Query: 220 G---DGVKEYSFQIAEMIGLGSDSEFLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIAT 279
           G   DGVK+Y+FQIAEMIGLGSD+EF QAG+R++LDIGCGFGSFGAHL+S+ VM +CIA 
Sbjct: 182 GLIFDGVKDYAFQIAEMIGLGSDTEFPQAGIRTVLDIGCGFGSFGAHLVSLNVMPICIAE 241

Query: 280 YEATGSQVQMALERGLPAMLGSFLTKQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADR 339
           YE +GSQVQ+ALERGLPAM+G+F +KQLPYP+LSFDMVHCAQCGI+W+ K  + L+E DR
Sbjct: 242 YETSGSQVQLALERGLPAMIGNFFSKQLPYPALSFDMVHCAQCGITWDIKDAMLLLEVDR 301

Query: 340 LLRPGGYFVLTSPTGKPVGGSLSSKKMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDP 399
           +L+PGGYFVLTSPT K  G S  +KK +I T ++E+++K+CW+   QQ ET++WQKT+DP
Sbjct: 302 VLKPGGYFVLTSPTSKAQGNSPDTKKTSISTRVDELSKKICWSLSGQQDETFLWQKTADP 361

Query: 400 HCYLSRKQEVVPLCKEGHDTPSYYQPLVPCISSTTS------------------------ 459
           +CY SR Q  +P+CK+    P YY PLVPCIS T S                        
Sbjct: 362 NCYSSRSQASIPVCKDDDSVP-YYHPLVPCISGTKSKRWIPIQNRSRASGTSLSELEIHG 421

Query: 460 VRSDDYSDELQIWRSALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGG 519
           ++ +++ +++Q+WRSALKNYWSLLTPLIFSDHPKRPGDEDP+PPF MIRN MDMNA YG 
Sbjct: 422 IKPEEFDEDIQVWRSALKNYWSLLTPLIFSDHPKRPGDEDPVPPFYMIRNAMDMNARYGN 481

Query: 520 LNAAFVEEKKTVWVMNVVPVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGL 579
           LN A + + K+VWVMNVVPV + NTLP+ILD+GF G LHDWCEPFPTYPRTYD+LHAN L
Sbjct: 482 LNQALLNQGKSVWVMNVVPVKARNTLPIILDRGFTGALHDWCEPFPTYPRTYDMLHANEL 541

Query: 580 LSQLLSSRCSMIGLLVEMDRILRPEGWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGS 626
           L+ L S RCS++ L +EMDRILRPEGWVVL DK+G IE AR LA ++RWEARVID Q+GS
Sbjct: 542 LTHLSSERCSLMDLFLEMDRILRPEGWVVLSDKLGVIEMARTLAARVRWEARVIDIQDGS 601

BLAST of Lag0040164 vs. ExPASy Swiss-Prot
Match: Q9C9Q8 (Probable pectin methyltransferase QUA2 OS=Arabidopsis thaliana OX=3702 GN=QUA2 PE=1 SV=2)

HSP 1 Score: 637.9 bits (1644), Expect = 1.2e-181
Identity = 313/607 (51.57%), Postives = 417/607 (68.70%), Query Frame = 0

Query: 54  LILCLISVLALIAVLGTSTSNAFD-SVTTTPVSDIYTSYRRQKERAANDLFDLKYLSLAT 113
           ++L L   L LI V+  + S  +  S++T+    +Y +YRR +E+  +DL+D+  +SL  
Sbjct: 81  MLLFLKISLVLIVVIALAGSFWWTISISTSSRGHVYHNYRRLQEQLVSDLWDIGEISLGP 140

Query: 114 TRLKEFGLCGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTTERCLVRPPKDYKIP 173
            R KE   C  E EN VPC+NV+ N+  GY  G+E DR C    + + CL  PP  Y++P
Sbjct: 141 NRWKELEYCNIESENFVPCFNVSENLALGYSNGDENDRFCGPG-SKQECLELPPVKYRVP 200

Query: 174 LSWPVGRDIIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSED--GDGVKEYSFQIA 233
           L WP G+DIIW  NVK+T  +++SSGS TKR+M++E++QI+F S     D V++YS QIA
Sbjct: 201 LRWPTGKDIIWHSNVKITAQEVVSSGSITKRMMMMEDDQISFRSASPMSDEVEDYSHQIA 260

Query: 234 EMIGLGSDSEFLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQVQMALERG 293
           EMIG+  D+ F++AGVR+ILDIGCG+GSFGAHL+S +++ MCIA YEA+GSQVQ+ LERG
Sbjct: 261 EMIGIKKDN-FIEAGVRTILDIGCGYGSFGAHLLSKQILTMCIANYEASGSQVQLTLERG 320

Query: 294 LPAMLGSFLTKQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYFVLTSPTG 353
           LPAM+GSF++KQLPYPSLSFDM+HC +CGI W+ K G+ L+E DR+L+PGGYFV TSP  
Sbjct: 321 LPAMIGSFISKQLPYPSLSFDMLHCLRCGIDWDQKDGLLLVEIDRVLKPGGYFVWTSPLT 380

Query: 354 KPVGGSLSSKKMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQEVVP-LC 413
            P       K+ N    + +    +CW  L QQ ET +W+KT +  CY SRK  V P +C
Sbjct: 381 NPRNKD-HLKRWNF---VHDFAESICWTLLNQQDETVVWKKTINTKCYSSRKPGVGPSVC 440

Query: 414 KEGHDTPS-YYQPLVPCISSTTS-------------VRSDDYSDELQI------------ 473
            +GHD  S YY+PL  CI  T S              RS+    EL +            
Sbjct: 441 TKGHDVESPYYRPLQMCIGGTRSRRWIPIEGRTRWPSRSNMNKTELSLYGLHPEVLGEDA 500

Query: 474 --WRSALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKK 533
             W+  ++ YWSLL+PLIFSDHPKRPGDEDP PP+NM+RNV+DMNA +GGLN+A +E +K
Sbjct: 501 ENWKITVREYWSLLSPLIFSDHPKRPGDEDPSPPYNMLRNVLDMNAQFGGLNSALLEARK 560

Query: 534 TVWVMNVVPVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLS---S 593
           +VWVMNVVP   PN LP+ILD+GF GVLH+WCEPFPTYPRTYDL+HA+ LLS   S    
Sbjct: 561 SVWVMNVVPTAGPNHLPMILDRGFVGVLHNWCEPFPTYPRTYDLVHADNLLSLQTSQPRK 620

Query: 594 RCSMIGLLVEMDRILRPEGWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLVC 626
            C +I +  E+DR+LRPEGWV+++D    +EKAR   TQ++WEARVI+ ++ S+QRLL+C
Sbjct: 621 TCLLIDIFTEIDRLLRPEGWVIIRDTAQLVEKARETITQLKWEARVIEVESSSEQRLLIC 680

BLAST of Lag0040164 vs. ExPASy Swiss-Prot
Match: O22285 (Probable methyltransferase PMT11 OS=Arabidopsis thaliana OX=3702 GN=At2g39750 PE=2 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 3.3e-94
Identity = 195/541 (36.04%), Postives = 289/541 (53.42%), Query Frame = 0

Query: 109 SLATTRLKEFGLCGKERENHVPCYNVT--ANMLSGYKEGEEYDRHCEVSRTTERCLVRPP 168
           S A  R+K+FG+C +    ++PC + T     L   + GE ++RHC        CLV PP
Sbjct: 168 SKARVRIKKFGMCPESMREYIPCLDNTDVIKKLKSTERGERFERHCPEKGKGLNCLVPPP 227

Query: 169 KDYKIPLSWPVGRDIIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAF---HSEDGDGVK 228
           K Y+ P+ WP  RD +W  NV  TR   L      +  +  ++N+  F    ++   G  
Sbjct: 228 KGYRQPIPWPKSRDEVWFSNVPHTR---LVEDKGGQNWISRDKNKFKFPGGGTQFIHGAD 287

Query: 229 EYSFQIAEMIGLGSDSEFLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQV 288
           +Y  Q+++M+   SD  F    +R  +D+GCG  SFGA+L+S  VM M +A  +   +Q+
Sbjct: 288 QYLDQMSKMV---SDITF-GKHIRVAMDVGCGVASFGAYLLSRDVMTMSVAPKDVHENQI 347

Query: 289 QMALERGLPAMLGSFLTKQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYF 348
           Q ALERG+PAM  +F T++L YPS +FD++HC++C I+W    GI L+E +R+LR GGYF
Sbjct: 348 QFALERGVPAMAAAFATRRLLYPSQAFDLIHCSRCRINWTRDDGILLLEINRMLRAGGYF 407

Query: 349 VLTSPTGKPVGGSLSSKKMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQ 408
              +        +L  +     T +  +T  LCW  + ++    IWQK  +  CYLSR+ 
Sbjct: 408 AWAAQPVYKHEPALEEQ----WTEMLNLTISLCWKLVKKEGYVAIWQKPFNNDCYLSREA 467

Query: 409 EV-VPLCKEGHDTPS-YYQPLVPCISSTTSVRSDDYSDELQIWRSALKNYWSLLTPLIFS 468
               PLC E  D  + +Y  L PCIS    +    Y   + +W + L      L  + F 
Sbjct: 468 GTKPPLCDESDDPDNVWYTNLKPCIS---RIPEKGYGGNVPLWPARLHTPPDRLQTIKFD 527

Query: 469 DHPKRP---------------GDEDPLPPFNM-IRNVMDMNAHYGGLNAAFVEEKKTVWV 528
            +  R                G    L    M +RNV+DM A +GG  AA  + K   WV
Sbjct: 528 SYIARKELFKAESKYWNEIIGGYVRALKWKKMKLRNVLDMRAGFGGFAAALNDHKLDCWV 587

Query: 529 MNVVPVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGL 588
           ++VVPV  PNTLP+I D+G  GV+HDWCEPF TYPRTYD LHA+GL S +   RC M  +
Sbjct: 588 LSVVPVSGPNTLPVIYDRGLLGVMHDWCEPFDTYPRTYDFLHASGLFS-IERKRCEMSTI 647

Query: 589 LVEMDRILRPEGWVVLKDKVGPIEKARMLATQIRWEARVIDSQNG--SDQRLLVCQKPFV 625
           L+EMDRILRP G   ++D +  +++ + +   + W   + D+  G  +  R+L C+K  +
Sbjct: 648 LLEMDRILRPGGRAYIRDSIDVMDEIQEITKAMGWHTSLRDTSEGPHASYRILTCEKRLL 693

BLAST of Lag0040164 vs. ExPASy Swiss-Prot
Match: Q940J9 (Probable methyltransferase PMT8 OS=Arabidopsis thaliana OX=3702 GN=At1g04430 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 6.9e-92
Identity = 180/535 (33.64%), Postives = 266/535 (49.72%), Query Frame = 0

Query: 116 KEFGLCGKERENHVPC----YNVTANMLSGYKEGEEYDRHCEVSRTTERCLVRPPKDYKI 175
           K F +C       +PC    +     +       E Y+RHC        CL+ PP  YK+
Sbjct: 82  KSFPVCDDRHSEIIPCLDRNFIYQMRLKLDLSLMEHYERHCPPPERRFNCLIPPPSGYKV 141

Query: 176 PLSWPVGRDIIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGD---GVKEYSFQ 235
           P+ WP  RD +W  N+  T    L+     +  M+ +  +I+F         G  +Y   
Sbjct: 142 PIKWPKSRDEVWKANIPHTH---LAKEKSDQNWMVEKGEKISFPGGGTHFHYGADKYIAS 201

Query: 236 IAEMIGLGSDSEFLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQVQMALE 295
           IA M+   +D    +  +R++LD+GCG  SFGA+L++  +M M +A  +   +Q+Q ALE
Sbjct: 202 IANMLNFSNDVLNDEGRLRTVLDVGCGVASFGAYLLASDIMTMSLAPNDVHQNQIQFALE 261

Query: 296 RGLPAMLGSFLTKQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYFVLTSP 355
           RG+PA LG   TK+LPYPS SF+  HC++C I W  + G+ L+E DR+LRPGGYF  +SP
Sbjct: 262 RGIPAYLGVLGTKRLPYPSRSFEFAHCSRCRIDWLQRDGLLLLELDRVLRPGGYFAYSSP 321

Query: 356 TGKPVGGSLSSKKMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQEV-VP 415
                  +   + + I   +  +  ++CW    ++ +T +WQK     CYL R+     P
Sbjct: 322 EAY----AQDEENLKIWKEMSALVERMCWRIAVKRNQTVVWQKPLSNDCYLEREPGTQPP 381

Query: 416 LCKEG--------------------HDTPSYYQPLVPCISSTTS---------VRSDDYS 475
           LC+                      HD  +    L P  +  TS           +D + 
Sbjct: 382 LCRSDADPDAVAGVSMEACITPYSKHDHKTKGSGLAPWPARLTSSPPRLADFGYSTDMFE 441

Query: 476 DELQIWRSALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVE 535
            + ++W+  + +YW+L++  + S               N +RN+MDM AH G   AA   
Sbjct: 442 KDTELWKQQVDSYWNLMSSKVKS---------------NTVRNIMDMKAHMGSFAAAL-- 501

Query: 536 EKKTVWVMNVVPVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSS 595
           + K VWVMNVV    PNTL LI D+G  G  H+WCE F TYPRTYDLLHA  + S + S 
Sbjct: 502 KDKDVWVMNVVSPDGPNTLKLIYDRGLIGTNHNWCEAFSTYPRTYDLLHAWSIFSDIKSK 561

Query: 596 RCSMIGLLVEMDRILRPEGWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQ 614
            CS   LL+EMDRILRP G+V+++DK   +E  +     + WE    +  N S +
Sbjct: 562 GCSAEDLLIEMDRILRPTGFVIIRDKQSVVESIKKYLQALHWETVASEKVNTSSE 592

BLAST of Lag0040164 vs. ExPASy TrEMBL
Match: A0A1S3BEJ3 (Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103489176 PE=3 SV=1)

HSP 1 Score: 1210.7 bits (3131), Expect = 0.0e+00
Identity = 590/649 (90.91%), Postives = 605/649 (93.22%), Query Frame = 0

Query: 1   MLYALLQDLNRRRFLFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60
           MLYAL +DLNRRRF FRVVLNWII+GTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS
Sbjct: 1   MLYALFRDLNRRRFPFRVVLNWIIKGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60

Query: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYTSYRRQKERAANDLFDLKYLSLATTRLKEFGL 120
           VLALIAVLGTSTSNAFDSVTTTPVSDIY SYRRQKERAA DLFDLK LSLATTRLKEFGL
Sbjct: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYASYRRQKERAAIDLFDLKSLSLATTRLKEFGL 120

Query: 121 CGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTTERCLVRPPKDYKIPLSWPVGRD 180
           CGKERENHVPCYNVTAN+L+GYKEGEEYDRHCEVSRT ERCLVRPPKDYKIPLSWPVGRD
Sbjct: 121 CGKERENHVPCYNVTANLLAGYKEGEEYDRHCEVSRTAERCLVRPPKDYKIPLSWPVGRD 180

Query: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240
           IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE
Sbjct: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240

Query: 241 FLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQVQMALERGLPAMLGSFLT 300
           F QAGVRSILDIGCGFGS GAHLIS+ VM MCIATYEATGSQVQMALERGLPAMLG+F T
Sbjct: 241 FFQAGVRSILDIGCGFGSLGAHLISLNVMAMCIATYEATGSQVQMALERGLPAMLGNFAT 300

Query: 301 KQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKPVGGSLSSK 360
           KQLPYPSLSFDMVHCAQC ISWNDKGGIFLIEADRLLRPGGYFVLTSPTGK +GGSLSSK
Sbjct: 301 KQLPYPSLSFDMVHCAQCDISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKTIGGSLSSK 360

Query: 361 KMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQ 420
           K NILTPLEEMT+KLCW  LAQQYETYIWQKT+DPHCY SRKQEVVPLCKE HDTPSYYQ
Sbjct: 361 KTNILTPLEEMTKKLCWILLAQQYETYIWQKTTDPHCYSSRKQEVVPLCKEAHDTPSYYQ 420

Query: 421 PLVPCISSTTS------------------------VRSDDYSDELQIWRSALKNYWSLLT 480
           PLVPC+SSTTS                        V+S+DYSDELQIWRSALKNYWSLLT
Sbjct: 421 PLVPCLSSTTSKRWIPIQNRSSGSHLSSAELEVHGVQSEDYSDELQIWRSALKNYWSLLT 480

Query: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNVVPVGSPNT 540
           PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVE+KKTVWVMNVVPVGSPNT
Sbjct: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEQKKTVWVMNVVPVGSPNT 540

Query: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEMDRILRPE 600
           LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRC+MIGLL+EMDRILRPE
Sbjct: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCNMIGLLLEMDRILRPE 600

Query: 601 GWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLVCQKPFVKK 626
           GWVVLKDKVGPIEK RMLATQIRWEARVID QNGSDQRLLVCQKPFVKK
Sbjct: 601 GWVVLKDKVGPIEKVRMLATQIRWEARVIDFQNGSDQRLLVCQKPFVKK 649

BLAST of Lag0040164 vs. ExPASy TrEMBL
Match: A0A0A0KW52 (Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G606620 PE=3 SV=1)

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 588/656 (89.63%), Postives = 606/656 (92.38%), Query Frame = 0

Query: 1   MLYALLQDLNRRRFLFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60
           MLYAL +DLN+RRF FRVVL+WI++GTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS
Sbjct: 1   MLYALFRDLNQRRFPFRVVLHWIVKGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60

Query: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYTSYRRQKERAANDLFDLKYLSLATTRLKEFGL 120
           VLALIAVLGTSTSNAFDSVTTTPVSDIY SYRRQKERAA DLFDLK LSLATTRLKEFGL
Sbjct: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYASYRRQKERAAIDLFDLKSLSLATTRLKEFGL 120

Query: 121 CGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTTERCLVRPPKDYKIPLSWPVGRD 180
           CGKERENHVPCYNVTAN+L+GYKEGEEYDRHCEVSRT +RCLVRPPKDYKIPLSWPVGRD
Sbjct: 121 CGKERENHVPCYNVTANLLAGYKEGEEYDRHCEVSRTAQRCLVRPPKDYKIPLSWPVGRD 180

Query: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240
           IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE
Sbjct: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240

Query: 241 FLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQVQMALERGLPAMLGSFLT 300
           F QAGVRSILDIGCGFGS GAHLIS+ VMVMCIATYEATGSQVQMALERGLPAMLG+F+T
Sbjct: 241 FFQAGVRSILDIGCGFGSLGAHLISLNVMVMCIATYEATGSQVQMALERGLPAMLGNFVT 300

Query: 301 KQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKPVGGSLSSK 360
           KQLPYPSLSFDMVHCAQC ISWNDKGGIFLIEADRLLRPGGYFVLTSPTGK +GGSLSSK
Sbjct: 301 KQLPYPSLSFDMVHCAQCDISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKTIGGSLSSK 360

Query: 361 KMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQ 420
           K NILTPLEEMT+KLCW  LAQQYETYIWQKT+DPHCY SRKQEVVPLCKE HDTPSYYQ
Sbjct: 361 KTNILTPLEEMTKKLCWILLAQQYETYIWQKTTDPHCYFSRKQEVVPLCKEAHDTPSYYQ 420

Query: 421 PLVPCISSTT-------------------------------SVRSDDYSDELQIWRSALK 480
           PLVPCISSTT                               SV+S+DYSDELQIW+SALK
Sbjct: 421 PLVPCISSTTSKRWIPIYNRSSGSHLSSAELEVHGKYSSVDSVQSEDYSDELQIWQSALK 480

Query: 481 NYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNVV 540
           NYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVE+KKTVWVMNVV
Sbjct: 481 NYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEQKKTVWVMNVV 540

Query: 541 PVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEM 600
           PVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEM
Sbjct: 541 PVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEM 600

Query: 601 DRILRPEGWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLVCQKPFVKK 626
           DRILRPEGWVV KDKVGPIEK RMLATQIRWEARVID QNGSDQRLLVCQKPFVKK
Sbjct: 601 DRILRPEGWVVFKDKVGPIEKVRMLATQIRWEARVIDFQNGSDQRLLVCQKPFVKK 656

BLAST of Lag0040164 vs. ExPASy TrEMBL
Match: A0A6J1G0X1 (Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111449665 PE=3 SV=1)

HSP 1 Score: 1194.9 bits (3090), Expect = 0.0e+00
Identity = 579/649 (89.21%), Postives = 603/649 (92.91%), Query Frame = 0

Query: 1   MLYALLQDLNRRRFLFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60
           MLYALL+DLNRRRF FRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPF+WLILCLIS
Sbjct: 1   MLYALLRDLNRRRFPFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFNWLILCLIS 60

Query: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYTSYRRQKERAANDLFDLKYLSLATTRLKEFGL 120
           VLALI VLGTSTSN FDSV+TTPVSDIYTSYRRQKERAA DLFDLK LSLAT RLKEFGL
Sbjct: 61  VLALIVVLGTSTSNVFDSVSTTPVSDIYTSYRRQKERAAIDLFDLKSLSLATGRLKEFGL 120

Query: 121 CGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTTERCLVRPPKDYKIPLSWPVGRD 180
           CGKERENHVPCYNVTAN L+GYKEGEEYDRHCEVSRT ERCLVRPPKDYKIPLSWPVGRD
Sbjct: 121 CGKERENHVPCYNVTANTLAGYKEGEEYDRHCEVSRTAERCLVRPPKDYKIPLSWPVGRD 180

Query: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240
           IIWSGN+KVT+DQLLSSGSPTKRLMLLEENQI+FHSEDGDGVKEYSFQIAEMIGLG+DSE
Sbjct: 181 IIWSGNLKVTKDQLLSSGSPTKRLMLLEENQISFHSEDGDGVKEYSFQIAEMIGLGTDSE 240

Query: 241 FLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQVQMALERGLPAMLGSFLT 300
           FLQAGVRSILDIGCGFGSFGAHL SM VMVMCIATYEATGSQVQMALERGLPAMLG+F+T
Sbjct: 241 FLQAGVRSILDIGCGFGSFGAHLTSMNVMVMCIATYEATGSQVQMALERGLPAMLGNFIT 300

Query: 301 KQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKPVGGSLSSK 360
           KQLPYPSLSFDMVHCAQC ISWNDKGGIFLIEADRLLRPGGYFVLTSPT K VGGSLSSK
Sbjct: 301 KQLPYPSLSFDMVHCAQCEISWNDKGGIFLIEADRLLRPGGYFVLTSPTSKAVGGSLSSK 360

Query: 361 KMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQ 420
           K++ILTPLEE+TR+LCW FLAQQYETYIWQKT+DP CYLS KQE+VPLCKEGHDTPSYYQ
Sbjct: 361 KISILTPLEEITRRLCWTFLAQQYETYIWQKTTDPRCYLSGKQEIVPLCKEGHDTPSYYQ 420

Query: 421 PLVPCISSTTS------------------------VRSDDYSDELQIWRSALKNYWSLLT 480
           PLVPC+SSTTS                        V+SDDY+D+LQIWR+ALKNYWSLLT
Sbjct: 421 PLVPCLSSTTSKRWIPIQNRSSSSRLSSAELEVHGVQSDDYADDLQIWRTALKNYWSLLT 480

Query: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNVVPVGSPNT 540
           PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVE+KKTVWVMNVVPVGSPNT
Sbjct: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEQKKTVWVMNVVPVGSPNT 540

Query: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEMDRILRPE 600
           LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSM+  LVEMDRILRPE
Sbjct: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMLEFLVEMDRILRPE 600

Query: 601 GWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLVCQKPFVKK 626
           GWV+L DKVGPIEKARML T IRWEARVID QNGSDQRLLVCQKPFVKK
Sbjct: 601 GWVILNDKVGPIEKARMLVTHIRWEARVIDFQNGSDQRLLVCQKPFVKK 649

BLAST of Lag0040164 vs. ExPASy TrEMBL
Match: A0A6J1HXM4 (Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111467573 PE=3 SV=1)

HSP 1 Score: 1189.1 bits (3075), Expect = 0.0e+00
Identity = 578/649 (89.06%), Postives = 601/649 (92.60%), Query Frame = 0

Query: 1   MLYALLQDLNRRRFLFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60
           MLYALL+DLNRRRF FRVV NWIIEGTSEILDMRSSWVNKISVILGSKPPF+WLILCLIS
Sbjct: 1   MLYALLRDLNRRRFPFRVVPNWIIEGTSEILDMRSSWVNKISVILGSKPPFNWLILCLIS 60

Query: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYTSYRRQKERAANDLFDLKYLSLATTRLKEFGL 120
           VLALI VLGTSTSNAFDSV+TTPVSDIYTSYRRQKERAA DLFDLK LSLAT RLKEFGL
Sbjct: 61  VLALIVVLGTSTSNAFDSVSTTPVSDIYTSYRRQKERAAIDLFDLKSLSLATGRLKEFGL 120

Query: 121 CGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTTERCLVRPPKDYKIPLSWPVGRD 180
           CGKERENHVPCYNVTANML+GYKEGEEYDRHCEVSRT E CLVRPPKDYKIPLSWPVGRD
Sbjct: 121 CGKERENHVPCYNVTANMLAGYKEGEEYDRHCEVSRTAECCLVRPPKDYKIPLSWPVGRD 180

Query: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240
           IIWSGN+KVT+DQLLSSGSPTKRLMLLEENQI+FHSEDGDGVKEYSFQIAEMIGLG+DSE
Sbjct: 181 IIWSGNLKVTKDQLLSSGSPTKRLMLLEENQISFHSEDGDGVKEYSFQIAEMIGLGTDSE 240

Query: 241 FLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQVQMALERGLPAMLGSFLT 300
           FLQAGVRSILDIGCGFGSFGAHL SM VMVMCIATYEATGSQVQMALERGLPAMLG+F+ 
Sbjct: 241 FLQAGVRSILDIGCGFGSFGAHLTSMNVMVMCIATYEATGSQVQMALERGLPAMLGNFIA 300

Query: 301 KQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKPVGGSLSSK 360
           KQLPYPSLSFDMVHCAQC ISWNDKGGIFLIEADRLLRPGGYFVLTSPT K VGGSLSSK
Sbjct: 301 KQLPYPSLSFDMVHCAQCEISWNDKGGIFLIEADRLLRPGGYFVLTSPTSKAVGGSLSSK 360

Query: 361 KMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQ 420
           K++ILTPLEE+TR+LCW FLAQQYETYIWQKT+DP CYLS KQE+VPLCKEGHDTPSYYQ
Sbjct: 361 KISILTPLEEVTRRLCWTFLAQQYETYIWQKTTDPRCYLSGKQEIVPLCKEGHDTPSYYQ 420

Query: 421 PLVPCISSTTS------------------------VRSDDYSDELQIWRSALKNYWSLLT 480
           PLVPC+SSTTS                        V+S+DYSD+LQIWR+ALKNYWSLLT
Sbjct: 421 PLVPCLSSTTSKRWIPIQNRSSSSRLSSAELEVHGVQSEDYSDDLQIWRTALKNYWSLLT 480

Query: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNVVPVGSPNT 540
           PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVE+KKTVWVMNVVPVGSPNT
Sbjct: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEQKKTVWVMNVVPVGSPNT 540

Query: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEMDRILRPE 600
           LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMI  LVEMDRILRPE
Sbjct: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIEFLVEMDRILRPE 600

Query: 601 GWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLVCQKPFVKK 626
           GWV+L DKVGPIEKARML T IRWEARVID QNGSDQRLLVCQKP VKK
Sbjct: 601 GWVILNDKVGPIEKARMLVTHIRWEARVIDFQNGSDQRLLVCQKPLVKK 649

BLAST of Lag0040164 vs. ExPASy TrEMBL
Match: A0A6J1D4P9 (Methyltransferase OS=Momordica charantia OX=3673 GN=LOC111017568 PE=3 SV=1)

HSP 1 Score: 1180.2 bits (3052), Expect = 0.0e+00
Identity = 573/649 (88.29%), Postives = 600/649 (92.45%), Query Frame = 0

Query: 1   MLYALLQDLNRRRFLFRVVLNWIIEGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60
           MLYALL+D+NRRRF FRV+LNWII+GTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS
Sbjct: 1   MLYALLRDINRRRFPFRVILNWIIDGTSEILDMRSSWVNKISVILGSKPPFSWLILCLIS 60

Query: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYTSYRRQKERAANDLFDLKYLSLATTRLKEFGL 120
           VLALIAVLGTSTSNAFDSVTTTPVSDIYTSYRRQKERAA DLFDLK LSL TTRLKEFGL
Sbjct: 61  VLALIAVLGTSTSNAFDSVTTTPVSDIYTSYRRQKERAAIDLFDLKSLSLGTTRLKEFGL 120

Query: 121 CGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTTERCLVRPPKDYKIPLSWPVGRD 180
           CGKERENHVPCYNVTANML+GY + +EYDRHCEVSR  +RCLVRPPKDYKIPLSWP GRD
Sbjct: 121 CGKERENHVPCYNVTANMLAGYIDEQEYDRHCEVSRAADRCLVRPPKDYKIPLSWPAGRD 180

Query: 181 IIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSEDGDGVKEYSFQIAEMIGLGSDSE 240
           IIWSGNVK+TR+QLLSSGSPTKRLMLLEENQI+FHSEDGDGVKEYSFQIAEMIGLGSDSE
Sbjct: 181 IIWSGNVKITREQLLSSGSPTKRLMLLEENQISFHSEDGDGVKEYSFQIAEMIGLGSDSE 240

Query: 241 FLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIATYEATGSQVQMALERGLPAMLGSFLT 300
           FLQAGVR+ILDIGCGFGSFGAHLISM VMVMCIATYEATGSQVQMALERGLPAMLG+F T
Sbjct: 241 FLQAGVRTILDIGCGFGSFGAHLISMNVMVMCIATYEATGSQVQMALERGLPAMLGNFNT 300

Query: 301 KQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADRLLRPGGYFVLTSPTGKPVGGSLSSK 360
           KQLPYPSLSFDMVHCAQCGISWNDKGG+FLIEADRLLRPGGYFVLT+PTGKPVG SLS+K
Sbjct: 301 KQLPYPSLSFDMVHCAQCGISWNDKGGMFLIEADRLLRPGGYFVLTTPTGKPVGSSLSTK 360

Query: 361 KMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQ 420
           ++NILTPLEEMT++LCW+ LAQQYETYIWQKT+DP CYLS KQEV+PLCKEGHDTPSYYQ
Sbjct: 361 RINILTPLEEMTQRLCWSLLAQQYETYIWQKTTDPQCYLSHKQEVLPLCKEGHDTPSYYQ 420

Query: 421 PLVPCISSTTS------------------------VRSDDYSDELQIWRSALKNYWSLLT 480
            LVPC+SSTTS                        VRS DYSD+LQIWRSALKNYWSLLT
Sbjct: 421 HLVPCLSSTTSKRWIPIQNRSSGSHLSSAELEVHGVRSADYSDDLQIWRSALKNYWSLLT 480

Query: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNVVPVGSPNT 540
           PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNVVPV S NT
Sbjct: 481 PLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNVVPVDSRNT 540

Query: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVEMDRILRPE 600
           LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHAN LLSQL SSRC MI LL+EMDRILRPE
Sbjct: 541 LPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANELLSQLSSSRCGMIELLLEMDRILRPE 600

Query: 601 GWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLVCQKPFVKK 626
           GWV+L DKVGPIEKARMLATQIRWEARVID QNGSDQRLLVCQKPFVKK
Sbjct: 601 GWVILHDKVGPIEKARMLATQIRWEARVIDIQNGSDQRLLVCQKPFVKK 649

BLAST of Lag0040164 vs. TAIR 10
Match: AT2G03480.2 (QUASIMODO2 LIKE 2 )

HSP 1 Score: 865.5 bits (2235), Expect = 2.5e-251
Identity = 404/597 (67.67%), Postives = 491/597 (82.24%), Query Frame = 0

Query: 33  MRSSWVNKISVILGSKPPFSWLILCLISVLALIAVLGTSTSNAFD-SVTTTPVSDIYTSY 92
           MR SW   +S + G +P    L+  ++ V+AL+ +L   TSN++D S ++T V +IY++Y
Sbjct: 1   MRGSWYKSVSSVFGLRPRIRGLLFFIVGVVALVTILAPLTSNSYDSSSSSTLVPNIYSNY 60

Query: 93  RRQKERAANDLFDLKYLSLATTRLKEFGLCGKERENHVPCYNVTANMLSGYKEGEEYDRH 152
           RR KE+AA D  DL+ LSL  + LKEF  CGKERE++VPCYN+T N+L+G +EGEE DRH
Sbjct: 61  RRIKEQAAVDYLDLRSLSLGAS-LKEFPFCGKERESYVPCYNITGNLLAGLQEGEELDRH 120

Query: 153 CEVSRTTERCLVRPPKDYKIPLSWPVGRDIIWSGNVKVTRDQLLSSGSPTKRLMLLEENQ 212
           CE  R  ERC+VRPP+DYKIPL WP+GRDIIWSGNVK+T+DQ LSSG+ T RLMLLEENQ
Sbjct: 121 CEFEREKERCVVRPPRDYKIPLRWPLGRDIIWSGNVKITKDQFLSSGTVTTRLMLLEENQ 180

Query: 213 IAFHSEDG---DGVKEYSFQIAEMIGLGSDSEFLQAGVRSILDIGCGFGSFGAHLISMKV 272
           I FHSEDG   DGVK+Y+ QIAEMIGLGSD+EF QAGVR++LDIGCGFGSFGAHL+S+K+
Sbjct: 181 ITFHSEDGLVFDGVKDYARQIAEMIGLGSDTEFAQAGVRTVLDIGCGFGSFGAHLVSLKL 240

Query: 273 MVMCIATYEATGSQVQMALERGLPAMLGSFLTKQLPYPSLSFDMVHCAQCGISWNDKGGI 332
           M +CIA YEATGSQVQ+ALERGLPAM+G+F +KQLPYP+LSFDMVHCAQCG +W+ K  +
Sbjct: 241 MPICIAEYEATGSQVQLALERGLPAMIGNFFSKQLPYPALSFDMVHCAQCGTTWDIKDAM 300

Query: 333 FLIEADRLLRPGGYFVLTSPTGKPVGGSLSSKKMNILTPLEEMTRKLCWNFLAQQYETYI 392
            L+E DR+L+PGGYFVLTSPT K  G    +KK +I T + E+++K+CW+  AQQ ET++
Sbjct: 301 LLLEVDRVLKPGGYFVLTSPTNKAQGNLPDTKKTSISTRVNELSKKICWSLTAQQDETFL 360

Query: 393 WQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQPLVPCISSTTSVRSDDYSDELQIWRSAL 452
           WQKTSD  CY SR Q  +PLCK+G   P YY PLVPCIS TTS++ +++ ++ QIWRSAL
Sbjct: 361 WQKTSDSSCYSSRSQASIPLCKDGDSVP-YYHPLVPCISGTTSLKPEEFFEDTQIWRSAL 420

Query: 453 KNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFVEEKKTVWVMNV 512
           KNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDM+A +G LNAA ++E K+ WVMNV
Sbjct: 421 KNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMHARFGNLNAALLDEGKSAWVMNV 480

Query: 513 VPVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLSSRCSMIGLLVE 572
           VPV + NTLP+ILD+GFAGVLHDWCEPFPTYPRTYD+LHAN LL+ L S RCS++ L +E
Sbjct: 481 VPVNARNTLPIILDRGFAGVLHDWCEPFPTYPRTYDMLHANELLTHLSSERCSLMDLFLE 540

Query: 573 MDRILRPEGWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLVCQKPFVKK 626
           MDRILRPEGWVVL DKVG IE AR LA ++RWEARVID Q+GSDQRLLVCQKPF+KK
Sbjct: 541 MDRILRPEGWVVLSDKVGVIEMARALAARVRWEARVIDLQDGSDQRLLVCQKPFIKK 595

BLAST of Lag0040164 vs. TAIR 10
Match: AT2G03480.1 (QUASIMODO2 LIKE 2 )

HSP 1 Score: 843.2 bits (2177), Expect = 1.4e-244
Identity = 404/608 (66.45%), Postives = 487/608 (80.10%), Query Frame = 0

Query: 33  MRSSWVNKISVILGSKPPFSWLILCLISVLALIAVLGTSTSNAFD-SVTTTPVSDIYTSY 92
           MR SW   +S + G +P    L+  ++ V+AL+ +L   TSN++D S ++T V +IY++Y
Sbjct: 1   MRGSWYKSVSSVFGLRPRIRGLLFFIVGVVALVTILAPLTSNSYDSSSSSTLVPNIYSNY 60

Query: 93  RRQKERAANDLFDLKYLSLATTRLKEFGLCGKERENHVPCYNVTANMLSGYKEGEEYDRH 152
           RR KE+AA D  DL+ LSL  + LKEF  CGKERE++VPCYN+T N+L+G +EGEE DRH
Sbjct: 61  RRIKEQAAVDYLDLRSLSLGAS-LKEFPFCGKERESYVPCYNITGNLLAGLQEGEELDRH 120

Query: 153 CEVSRTTERCLVRPPKDYKIPLSWPVGRDIIWSGNVKVTRDQLLSSGSPTKRLMLLEENQ 212
           CE  R  ERC+VRPP+DYKIPL WP+GRDIIWSGNVK+T+DQ LSSG+ T RLMLLEENQ
Sbjct: 121 CEFEREKERCVVRPPRDYKIPLRWPLGRDIIWSGNVKITKDQFLSSGTVTTRLMLLEENQ 180

Query: 213 IAFHSEDG---DGVKEYSFQIAEMIGLGSDSEFLQAGVRSILDIGCGFGSFGAHLISMKV 272
           I FHSEDG   DGVK+Y+ QIAEMIGLGSD+EF QAGVR++LDIGCGFGSFGAHL+S+K+
Sbjct: 181 ITFHSEDGLVFDGVKDYARQIAEMIGLGSDTEFAQAGVRTVLDIGCGFGSFGAHLVSLKL 240

Query: 273 MVMCIATYEATGSQVQMALERGLPAMLGSFLTKQLPYPSLSFDMVHCAQCGISWNDKGGI 332
           M +CIA YEATGSQVQ+ALERGLPAM+G+F +KQLPYP+LSFDMVHCAQCG +W+ K  +
Sbjct: 241 MPICIAEYEATGSQVQLALERGLPAMIGNFFSKQLPYPALSFDMVHCAQCGTTWDIKDAM 300

Query: 333 FLIEADRLLRPGGYFVLTSPTGKPVGGSLSSKKMNILTPLEEMTRKLCWNFLAQQYETYI 392
            L+E DR+L+PGGYFVLTSPT K  G    +KK +I T + E+++K+CW+  AQQ ET++
Sbjct: 301 LLLEVDRVLKPGGYFVLTSPTNKAQGNLPDTKKTSISTRVNELSKKICWSLTAQQDETFL 360

Query: 393 WQKTSDPHCYLSRKQEVVPLCKEGHDTPSYYQPLVPCISSTTSVR----------SDDYS 452
           WQKTSD  CY SR Q  +PLCK+G   P YY PLVPCIS TTS R          +   S
Sbjct: 361 WQKTSDSSCYSSRSQASIPLCKDGDSVP-YYHPLVPCISGTTSKRWISIQNRSAVAGTTS 420

Query: 453 DELQI-WRSALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGGLNAAFV 512
             L+I  +SALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDM+A +G LNAA +
Sbjct: 421 AGLEIHGKSALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMHARFGNLNAALL 480

Query: 513 EEKKTVWVMNVVPVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGLLSQLLS 572
           +E K+ WVMNVVPV + NTLP+ILD+GFAGVLHDWCEPFPTYPRTYD+LHAN LL+ L S
Sbjct: 481 DEGKSAWVMNVVPVNARNTLPIILDRGFAGVLHDWCEPFPTYPRTYDMLHANELLTHLSS 540

Query: 573 SRCSMIGLLVEMDRILRPEGWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGSDQRLLV 626
            RCS++ L +EMDRILRPEGWVVL DKVG IE AR LA ++RWEARVID Q+GSDQRLLV
Sbjct: 541 ERCSLMDLFLEMDRILRPEGWVVLSDKVGVIEMARALAARVRWEARVIDLQDGSDQRLLV 600

BLAST of Lag0040164 vs. TAIR 10
Match: AT1G13860.4 (QUASIMODO2 LIKE 1 )

HSP 1 Score: 813.5 bits (2100), Expect = 1.1e-235
Identity = 385/614 (62.70%), Postives = 475/614 (77.36%), Query Frame = 0

Query: 40  KISVILGSKPPFSWLILCLISVLALIAVL-GTSTSNAFDSVTTTPVSDIYTSYRRQKERA 99
           K++ ++G +P  S L+   + V+ALI +L   S S++  S T  P S+IY++Y R KE+A
Sbjct: 2   KVASVIGLRPRISGLLFLTLGVIALITILVPNSDSSSTTSTTRVPPSNIYSNYGRVKEQA 61

Query: 100 ANDLFDLKYLSLATTRLKEFGLCGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTT 159
           A D  DL++ SL   RLKEF LCGKER+N+VPCYNVT           E DR+CE +R  
Sbjct: 62  AVDYLDLRFFSLGVNRLKEFPLCGKERDNYVPCYNVT-----------ESDRNCEFAREE 121

Query: 160 ERCLVRPPKDYKIPLSWPVGRDIIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSED 219
           ERCLVRPP+DYKIPL WPVGRDIIW+GNVK+T+DQ LSSG+ TKRLMLLEENQI FHS+D
Sbjct: 122 ERCLVRPPRDYKIPLRWPVGRDIIWTGNVKITKDQFLSSGTMTKRLMLLEENQITFHSDD 181

Query: 220 G---DGVKEYSFQIAEMIGLGSDSEFLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIAT 279
           G   DGVK+Y+FQIAEMIGLGSD+EF QAG+R++LDIGCGFGSFGAHL+S+ VM +CIA 
Sbjct: 182 GLIFDGVKDYAFQIAEMIGLGSDTEFPQAGIRTVLDIGCGFGSFGAHLVSLNVMPICIAE 241

Query: 280 YEATGSQVQMALERGLPAMLGSFLTKQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADR 339
           YE +GSQVQ+ALERGLPAM+G+F +KQLPYP+LSFDMVHCAQCGI+W+ K  + L+E DR
Sbjct: 242 YETSGSQVQLALERGLPAMIGNFFSKQLPYPALSFDMVHCAQCGITWDIKDAMLLLEVDR 301

Query: 340 LLRPGGYFVLTSPTGKPVGGSLSSKKMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDP 399
           +L+PGGYFVLTSPT K  G S  +KK +I T ++E+++K+CW+   QQ ET++WQKT+DP
Sbjct: 302 VLKPGGYFVLTSPTSKAQGNSPDTKKTSISTRVDELSKKICWSLSGQQDETFLWQKTADP 361

Query: 400 HCYLSRKQEVVPLCKEGHDTPSYYQPLVPCISSTTS------------------------ 459
           +CY SR Q  +P+CK+    P YY PLVPCIS T S                        
Sbjct: 362 NCYSSRSQASIPVCKDDDSVP-YYHPLVPCISGTKSKRWIPIQNRSRASGTSLSELEIHG 421

Query: 460 VRSDDYSDELQIWRSALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGG 519
           ++ +++ +++Q+WRSALKNYWSLLTPLIFSDHPKRPGDEDP+PPF MIRN MDMNA YG 
Sbjct: 422 IKPEEFDEDIQVWRSALKNYWSLLTPLIFSDHPKRPGDEDPVPPFYMIRNAMDMNARYGN 481

Query: 520 LNAAFVEEKKTVWVMNVVPVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGL 579
           LN A + + K+VWVMNVVPV + NTLP+ILD+GF G LHDWCEPFPTYPRTYD+LHAN L
Sbjct: 482 LNQALLNQGKSVWVMNVVPVKARNTLPIILDRGFTGALHDWCEPFPTYPRTYDMLHANEL 541

Query: 580 LSQLLSSRCSMIGLLVEMDRILRPEGWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGS 626
           L+ L S RCS++ L +EMDRILRPEGWVVL DK+G IE AR LA ++RWEARVID Q+GS
Sbjct: 542 LTHLSSERCSLMDLFLEMDRILRPEGWVVLSDKLGVIEMARTLAARVRWEARVIDIQDGS 601

BLAST of Lag0040164 vs. TAIR 10
Match: AT1G13860.1 (QUASIMODO2 LIKE 1 )

HSP 1 Score: 813.5 bits (2100), Expect = 1.1e-235
Identity = 385/614 (62.70%), Postives = 475/614 (77.36%), Query Frame = 0

Query: 40  KISVILGSKPPFSWLILCLISVLALIAVL-GTSTSNAFDSVTTTPVSDIYTSYRRQKERA 99
           K++ ++G +P  S L+   + V+ALI +L   S S++  S T  P S+IY++Y R KE+A
Sbjct: 2   KVASVIGLRPRISGLLFLTLGVIALITILVPNSDSSSTTSTTRVPPSNIYSNYGRVKEQA 61

Query: 100 ANDLFDLKYLSLATTRLKEFGLCGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTT 159
           A D  DL++ SL   RLKEF LCGKER+N+VPCYNVT           E DR+CE +R  
Sbjct: 62  AVDYLDLRFFSLGVNRLKEFPLCGKERDNYVPCYNVT-----------ESDRNCEFAREE 121

Query: 160 ERCLVRPPKDYKIPLSWPVGRDIIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSED 219
           ERCLVRPP+DYKIPL WPVGRDIIW+GNVK+T+DQ LSSG+ TKRLMLLEENQI FHS+D
Sbjct: 122 ERCLVRPPRDYKIPLRWPVGRDIIWTGNVKITKDQFLSSGTMTKRLMLLEENQITFHSDD 181

Query: 220 G---DGVKEYSFQIAEMIGLGSDSEFLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIAT 279
           G   DGVK+Y+FQIAEMIGLGSD+EF QAG+R++LDIGCGFGSFGAHL+S+ VM +CIA 
Sbjct: 182 GLIFDGVKDYAFQIAEMIGLGSDTEFPQAGIRTVLDIGCGFGSFGAHLVSLNVMPICIAE 241

Query: 280 YEATGSQVQMALERGLPAMLGSFLTKQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADR 339
           YE +GSQVQ+ALERGLPAM+G+F +KQLPYP+LSFDMVHCAQCGI+W+ K  + L+E DR
Sbjct: 242 YETSGSQVQLALERGLPAMIGNFFSKQLPYPALSFDMVHCAQCGITWDIKDAMLLLEVDR 301

Query: 340 LLRPGGYFVLTSPTGKPVGGSLSSKKMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDP 399
           +L+PGGYFVLTSPT K  G S  +KK +I T ++E+++K+CW+   QQ ET++WQKT+DP
Sbjct: 302 VLKPGGYFVLTSPTSKAQGNSPDTKKTSISTRVDELSKKICWSLSGQQDETFLWQKTADP 361

Query: 400 HCYLSRKQEVVPLCKEGHDTPSYYQPLVPCISSTTS------------------------ 459
           +CY SR Q  +P+CK+    P YY PLVPCIS T S                        
Sbjct: 362 NCYSSRSQASIPVCKDDDSVP-YYHPLVPCISGTKSKRWIPIQNRSRASGTSLSELEIHG 421

Query: 460 VRSDDYSDELQIWRSALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGG 519
           ++ +++ +++Q+WRSALKNYWSLLTPLIFSDHPKRPGDEDP+PPF MIRN MDMNA YG 
Sbjct: 422 IKPEEFDEDIQVWRSALKNYWSLLTPLIFSDHPKRPGDEDPVPPFYMIRNAMDMNARYGN 481

Query: 520 LNAAFVEEKKTVWVMNVVPVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGL 579
           LN A + + K+VWVMNVVPV + NTLP+ILD+GF G LHDWCEPFPTYPRTYD+LHAN L
Sbjct: 482 LNQALLNQGKSVWVMNVVPVKARNTLPIILDRGFTGALHDWCEPFPTYPRTYDMLHANEL 541

Query: 580 LSQLLSSRCSMIGLLVEMDRILRPEGWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGS 626
           L+ L S RCS++ L +EMDRILRPEGWVVL DK+G IE AR LA ++RWEARVID Q+GS
Sbjct: 542 LTHLSSERCSLMDLFLEMDRILRPEGWVVLSDKLGVIEMARTLAARVRWEARVIDIQDGS 601

BLAST of Lag0040164 vs. TAIR 10
Match: AT1G13860.3 (QUASIMODO2 LIKE 1 )

HSP 1 Score: 813.5 bits (2100), Expect = 1.1e-235
Identity = 385/614 (62.70%), Postives = 475/614 (77.36%), Query Frame = 0

Query: 40  KISVILGSKPPFSWLILCLISVLALIAVL-GTSTSNAFDSVTTTPVSDIYTSYRRQKERA 99
           K++ ++G +P  S L+   + V+ALI +L   S S++  S T  P S+IY++Y R KE+A
Sbjct: 2   KVASVIGLRPRISGLLFLTLGVIALITILVPNSDSSSTTSTTRVPPSNIYSNYGRVKEQA 61

Query: 100 ANDLFDLKYLSLATTRLKEFGLCGKERENHVPCYNVTANMLSGYKEGEEYDRHCEVSRTT 159
           A D  DL++ SL   RLKEF LCGKER+N+VPCYNVT           E DR+CE +R  
Sbjct: 62  AVDYLDLRFFSLGVNRLKEFPLCGKERDNYVPCYNVT-----------ESDRNCEFAREE 121

Query: 160 ERCLVRPPKDYKIPLSWPVGRDIIWSGNVKVTRDQLLSSGSPTKRLMLLEENQIAFHSED 219
           ERCLVRPP+DYKIPL WPVGRDIIW+GNVK+T+DQ LSSG+ TKRLMLLEENQI FHS+D
Sbjct: 122 ERCLVRPPRDYKIPLRWPVGRDIIWTGNVKITKDQFLSSGTMTKRLMLLEENQITFHSDD 181

Query: 220 G---DGVKEYSFQIAEMIGLGSDSEFLQAGVRSILDIGCGFGSFGAHLISMKVMVMCIAT 279
           G   DGVK+Y+FQIAEMIGLGSD+EF QAG+R++LDIGCGFGSFGAHL+S+ VM +CIA 
Sbjct: 182 GLIFDGVKDYAFQIAEMIGLGSDTEFPQAGIRTVLDIGCGFGSFGAHLVSLNVMPICIAE 241

Query: 280 YEATGSQVQMALERGLPAMLGSFLTKQLPYPSLSFDMVHCAQCGISWNDKGGIFLIEADR 339
           YE +GSQVQ+ALERGLPAM+G+F +KQLPYP+LSFDMVHCAQCGI+W+ K  + L+E DR
Sbjct: 242 YETSGSQVQLALERGLPAMIGNFFSKQLPYPALSFDMVHCAQCGITWDIKDAMLLLEVDR 301

Query: 340 LLRPGGYFVLTSPTGKPVGGSLSSKKMNILTPLEEMTRKLCWNFLAQQYETYIWQKTSDP 399
           +L+PGGYFVLTSPT K  G S  +KK +I T ++E+++K+CW+   QQ ET++WQKT+DP
Sbjct: 302 VLKPGGYFVLTSPTSKAQGNSPDTKKTSISTRVDELSKKICWSLSGQQDETFLWQKTADP 361

Query: 400 HCYLSRKQEVVPLCKEGHDTPSYYQPLVPCISSTTS------------------------ 459
           +CY SR Q  +P+CK+    P YY PLVPCIS T S                        
Sbjct: 362 NCYSSRSQASIPVCKDDDSVP-YYHPLVPCISGTKSKRWIPIQNRSRASGTSLSELEIHG 421

Query: 460 VRSDDYSDELQIWRSALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMNAHYGG 519
           ++ +++ +++Q+WRSALKNYWSLLTPLIFSDHPKRPGDEDP+PPF MIRN MDMNA YG 
Sbjct: 422 IKPEEFDEDIQVWRSALKNYWSLLTPLIFSDHPKRPGDEDPVPPFYMIRNAMDMNARYGN 481

Query: 520 LNAAFVEEKKTVWVMNVVPVGSPNTLPLILDQGFAGVLHDWCEPFPTYPRTYDLLHANGL 579
           LN A + + K+VWVMNVVPV + NTLP+ILD+GF G LHDWCEPFPTYPRTYD+LHAN L
Sbjct: 482 LNQALLNQGKSVWVMNVVPVKARNTLPIILDRGFTGALHDWCEPFPTYPRTYDMLHANEL 541

Query: 580 LSQLLSSRCSMIGLLVEMDRILRPEGWVVLKDKVGPIEKARMLATQIRWEARVIDSQNGS 626
           L+ L S RCS++ L +EMDRILRPEGWVVL DK+G IE AR LA ++RWEARVID Q+GS
Sbjct: 542 LTHLSSERCSLMDLFLEMDRILRPEGWVVLSDKLGVIEMARTLAARVRWEARVIDIQDGS 601

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038892405.10.0e+0091.68probable methyltransferase PMT5 [Benincasa hispida] >XP_038892406.1 probable met... [more]
XP_008446426.10.0e+0090.91PREDICTED: probable methyltransferase PMT5 [Cucumis melo] >XP_008446427.1 PREDIC... [more]
XP_031740879.10.0e+0090.60probable methyltransferase PMT5 [Cucumis sativus][more]
XP_023541770.10.0e+0089.37probable methyltransferase PMT5 [Cucurbita pepo subsp. pepo] >XP_023541771.1 pro... [more]
XP_022945429.10.0e+0089.21probable methyltransferase PMT5 [Cucurbita moschata] >XP_022945430.1 probable me... [more]
Match NameE-valueIdentityDescription
Q3EC771.9e-24366.45Probable methyltransferase PMT5 OS=Arabidopsis thaliana OX=3702 GN=At2g03480 PE=... [more]
Q8GYW91.6e-23462.70Probable methyltransferase PMT4 OS=Arabidopsis thaliana OX=3702 GN=At1g13860 PE=... [more]
Q9C9Q81.2e-18151.57Probable pectin methyltransferase QUA2 OS=Arabidopsis thaliana OX=3702 GN=QUA2 P... [more]
O222853.3e-9436.04Probable methyltransferase PMT11 OS=Arabidopsis thaliana OX=3702 GN=At2g39750 PE... [more]
Q940J96.9e-9233.64Probable methyltransferase PMT8 OS=Arabidopsis thaliana OX=3702 GN=At1g04430 PE=... [more]
Match NameE-valueIdentityDescription
A0A1S3BEJ30.0e+0090.91Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103489176 PE=3 SV=1[more]
A0A0A0KW520.0e+0089.63Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G606620 PE=3 SV=1[more]
A0A6J1G0X10.0e+0089.21Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111449665 PE=3 SV=1[more]
A0A6J1HXM40.0e+0089.06Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111467573 PE=3 SV=1[more]
A0A6J1D4P90.0e+0088.29Methyltransferase OS=Momordica charantia OX=3673 GN=LOC111017568 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G03480.22.5e-25167.67QUASIMODO2 LIKE 2 [more]
AT2G03480.11.4e-24466.45QUASIMODO2 LIKE 2 [more]
AT1G13860.41.1e-23562.70QUASIMODO2 LIKE 1 [more]
AT1G13860.11.1e-23562.70QUASIMODO2 LIKE 1 [more]
AT1G13860.31.1e-23562.70QUASIMODO2 LIKE 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePFAMPF03141Methyltransf_29coord: 478..620
e-value: 2.5E-53
score: 181.4
coord: 127..452
e-value: 9.5E-79
score: 265.3
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePANTHERPTHR10108SAM-DEPENDENT METHYLTRANSFERASEcoord: 33..624
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 229..414
e-value: 1.5E-13
score: 52.4
NoneNo IPR availablePANTHERPTHR10108:SF1083METHYLTRANSFERASE PMT4-RELATEDcoord: 33..624
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 249..346
e-value: 1.32214E-4
score: 39.7207
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 445..587
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 245..356

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040164.1Lag0040164.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0008168 methyltransferase activity