Lag0040147 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040147
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionWRKY DNA-binding protein 4
Locationchr13: 2517778 .. 2520759 (-)
RNA-Seq ExpressionLag0040147
SyntenyLag0040147
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGATGATGAAGAACAGCTGCCACCGTCGTCAGCGCCGCCTCCGCCGTCGAAATCTAAGGCACTTCCGTTGCGGCCAACTATTAATCTGCCGCCAAGGACCTGTATGGAGTCGCTCTTCAGCGGTGGGCCGGGTTTGGGAAGCGGGTTTAGCCCAGGACCCATGACCCTCGTCTCGAGCTTCTTCTCCGATTCCGACGACTGCAAGTCTTTTTCTCAGCTCCTCGCCGGAGCTATGGCGTCGCCGGTGGCTGTTCCTCCGAGGGCGTCTGAGCTCAAGGGCCCTGCCGGTCTGCTCGATTCTCCGGGGCTTTTTTCTCCCGGTCAGGTAATTGCTTAAAATTCACTTTGCGAACTGATTCTCTTCTTTGTCGGAGTTTTGCAAATGAAAATTCCCGTTGAATTGAGTGTGGTGTATGATTACGGAGTGCTTGAATAGTTGCAGGACAATTATGAGAAAAATCCGGATCATGTTTTCTTTAGTGTTTTTTGGCTTTTGGAAACAATTGATTCTGCATATTGAAACAGAGAAGAAATCATTTCATTTCGCTCCATTTTTCTCAAATGGATGTCGGTTTCTTTCTATTTCGAGCTTGTTCATAGTGGAAGAAGCAAAATAGTTGTATTAATGATGAGAACTGATTCGTTTATTTTGATCCTGCTTTATTTGCAGATTGAACTATAGCCCCTTTTGAAAATTTCAAGTTTTGAATTGTCTTAGAAGAAATAGAGCTGTGCATTCATCGTTAGATGAATTAAAATGACAATGTATGGAATGCGGCCTTTGGATGGATGTTTCTCTTTGTATTCTAATCAATGATCTCTTCAAGAAGCCTGTTATGTCGTAATCTATGTGATTAGTGAAACATTATAGCTATGGTTTCTACTTAACTTGTGTGACTTACTCCATTGTAGTCGAGATGAAGCAGATTAGCTGTTAAGCATGTGTGACATCTCCTTAAGGATCGAAGCTTATTTCAGGCTGAAGATTTTCATTCATGCTTATATAACTATATTACTTATTCGACGGGTTTGATGACAGTGATTCTTGGTACTGAATGAATTTATCATATAAAGTTGCATATGACATTTTTGTTGTTTATTATTGTATGGCTTTATTATACAAGACTGTTTATATAATGGCTTTCTCACTTTTTCACTCATGGCTTTGTTTTAGGGACCTTTTGAAATGACACATCAGCAGGCTTTGGCTCAGGTTACTATGCAGGCTGCACAGACCTATTCTCATAAGCAAATACAAGGAGAATCTTTTTCTTCATTGGTACCACCCTCTGCATCCTCCTCAGAGCTTTTGACAACCTTTCCGGGCGAGAAGACTAAAGATCAGCCGGTGCCACCTTCATTGCTGAACTCTGCGGTTGCATCCAAGGAATCATCGGATATCTCTCAATCAGATCAGAGATTGCAATCTTCTTCGTGTAATGTCGATAGGCCTGCTGATGATGGCTACAATTGGAGAAAATATGGACAGAAACAAGTGAAGGGCAGTGAATTTCCTCGAAGTTACTACAAGTGCACGCACCCGAATTGTCCTGTCAAGAAAAAGGTCGAACGATCTCTGGAAGGTCAAGTCATTGAAATTATCTATAAGGGTGAGCACAACCACAAACGACCTCAACCGAACAAACGGGCAAAGGATGTTGGAAATTCGAATGGATGTTCAAGTATTCATGGAAATCCTGAGCTGTCTTCTCAAGTTCAGAGTGGATATTTGAATAAATTAAATGATCAGCAGACGGCTGTGTCTTCCATTGCAAAGAAGGATCAAGAATCGAGTCATGTTACACATGAACAGTTGTCCAGTACTAGTGATGGTGAGGGAGGGAGTGAGATAGAAACTGGAGTGAATCGAAAAGATGAAGACGAACCTGACGCCAAGAGACGGTAAGGCACATTTATGAATAAGAAACCTTTTGAATTCACAATCATATAGCTGTTTTTTGTTTCTTGATTGATATGCACAATATTCTTCCTCTATTGTGCAGGAATACTGAGGTCAGAGTTTCTGAGCCAGCAACTTCTCATAGGACGCTCACAGAATCCAGAATCATTGTGCAGACTACAAGTGAAGTCGATCTATTGGATGATGGCTACAGGTGGCGAAAGTATGGGCAGAAAGTTGTTAAAGGCAACCCTTACCCCAGGTGAGTTTGTTTTATATCACATTCAGCTTTAGTAGTACGAGTTATAGAAACACAGATTGCTATTGTATCAAAAAAAACAGAGTGGTCTTAAAAATTGGAACTTAGTCTTAGTCGTGTTTGATTATCATTTGGTTTTTGGTTTTAAAAATTAAGCTTATAAACACTATTTCTACATGTAAGTTTCTTTGCTTTGTTATCTACCTTATTTCCATGTTTTCAAAAACCAAGTCAAATTTTGAAAACTACAAAAATAGTTTTCAAAAACTTGTTTTTGTTTTTGGAATTTGGCTAGGAACTCGAATTGTCCTTAACAAAGATAAAAACCATGGTAAGGAAATTGAGAGAAAACAAGCTTAATTTTAAAAAGCCAAATGATTACCAAACGAGGCCTTAAAAACTAACATCTTGATGATTGTAATTTGAAATATTGGGGCTGATTTGGCTGGTGACTAATAACTATGCGAATCCTTATGTGTGCTTAAGATGAAGTTGATTATCTTGGGGATTGCTTTTCTAGTTTTATCAAGTGATCTTCATTCCATAACAGGAGCTACTATAAATGCACAACTCCGGGATGCAATGTTCGTAAACATGTTGAGAGAGCTTCGACAGACCAAAAAGCTGTCATAACAACGTATGAGGGCAAACACAATCATGATGTTCCAGCTGGTAAAATCAGCAGCCACAGTACAGTCAACAGTAACGTTCCTCAGTTAAAATCACAAAATATAGCTACTGATAACAACACCGATCCTGGGAACTCTCGTCAACAGCCTGTTGGACTTCTACGATTAAAGAAAGAACAAATAACATAG

mRNA sequence

ATGGGTGATGATGAAGAACAGCTGCCACCGTCGTCAGCGCCGCCTCCGCCGTCGAAATCTAAGGCACTTCCGTTGCGGCCAACTATTAATCTGCCGCCAAGGACCTGTATGGAGTCGCTCTTCAGCGGTGGGCCGGGTTTGGGAAGCGGGTTTAGCCCAGGACCCATGACCCTCGTCTCGAGCTTCTTCTCCGATTCCGACGACTGCAAGTCTTTTTCTCAGCTCCTCGCCGGAGCTATGGCGTCGCCGGTGGCTGTTCCTCCGAGGGCGTCTGAGCTCAAGGGCCCTGCCGGTCTGCTCGATTCTCCGGGGCTTTTTTCTCCCGGTCAGGGACCTTTTGAAATGACACATCAGCAGGCTTTGGCTCAGGTTACTATGCAGGCTGCACAGACCTATTCTCATAAGCAAATACAAGGAGAATCTTTTTCTTCATTGGTACCACCCTCTGCATCCTCCTCAGAGCTTTTGACAACCTTTCCGGGCGAGAAGACTAAAGATCAGCCGGTGCCACCTTCATTGCTGAACTCTGCGGTTGCATCCAAGGAATCATCGGATATCTCTCAATCAGATCAGAGATTGCAATCTTCTTCGTGTAATGTCGATAGGCCTGCTGATGATGGCTACAATTGGAGAAAATATGGACAGAAACAAGTGAAGGGCAGTGAATTTCCTCGAAGTTACTACAAGTGCACGCACCCGAATTGTCCTGTCAAGAAAAAGGTCGAACGATCTCTGGAAGGTCAAGTCATTGAAATTATCTATAAGGGTGAGCACAACCACAAACGACCTCAACCGAACAAACGGGCAAAGGATGTTGGAAATTCGAATGGATGTTCAAGTATTCATGGAAATCCTGAGCTGTCTTCTCAAGTTCAGAGTGGATATTTGAATAAATTAAATGATCAGCAGACGGCTGTGTCTTCCATTGCAAAGAAGGATCAAGAATCGAGTCATGTTACACATGAACAGTTGTCCAGTACTAGTGATGGTGAGGGAGGGAGTGAGATAGAAACTGGAGTGAATCGAAAAGATGAAGACGAACCTGACGCCAAGAGACGGAATACTGAGGTCAGAGTTTCTGAGCCAGCAACTTCTCATAGGACGCTCACAGAATCCAGAATCATTGTGCAGACTACAAGTGAAGTCGATCTATTGGATGATGGCTACAGGTGGCGAAAGTATGGGCAGAAAGTTGTTAAAGGCAACCCTTACCCCAGGAGCTACTATAAATGCACAACTCCGGGATGCAATGTTCGTAAACATGTTGAGAGAGCTTCGACAGACCAAAAAGCTGTCATAACAACGTATGAGGGCAAACACAATCATGATGTTCCAGCTGGTAAAATCAGCAGCCACAGTACAGTCAACAGTAACGTTCCTCAGTTAAAATCACAAAATATAGCTACTGATAACAACACCGATCCTGGGAACTCTCGTCAACAGCCTGTTGGACTTCTACGATTAAAGAAAGAACAAATAACATAG

Coding sequence (CDS)

ATGGGTGATGATGAAGAACAGCTGCCACCGTCGTCAGCGCCGCCTCCGCCGTCGAAATCTAAGGCACTTCCGTTGCGGCCAACTATTAATCTGCCGCCAAGGACCTGTATGGAGTCGCTCTTCAGCGGTGGGCCGGGTTTGGGAAGCGGGTTTAGCCCAGGACCCATGACCCTCGTCTCGAGCTTCTTCTCCGATTCCGACGACTGCAAGTCTTTTTCTCAGCTCCTCGCCGGAGCTATGGCGTCGCCGGTGGCTGTTCCTCCGAGGGCGTCTGAGCTCAAGGGCCCTGCCGGTCTGCTCGATTCTCCGGGGCTTTTTTCTCCCGGTCAGGGACCTTTTGAAATGACACATCAGCAGGCTTTGGCTCAGGTTACTATGCAGGCTGCACAGACCTATTCTCATAAGCAAATACAAGGAGAATCTTTTTCTTCATTGGTACCACCCTCTGCATCCTCCTCAGAGCTTTTGACAACCTTTCCGGGCGAGAAGACTAAAGATCAGCCGGTGCCACCTTCATTGCTGAACTCTGCGGTTGCATCCAAGGAATCATCGGATATCTCTCAATCAGATCAGAGATTGCAATCTTCTTCGTGTAATGTCGATAGGCCTGCTGATGATGGCTACAATTGGAGAAAATATGGACAGAAACAAGTGAAGGGCAGTGAATTTCCTCGAAGTTACTACAAGTGCACGCACCCGAATTGTCCTGTCAAGAAAAAGGTCGAACGATCTCTGGAAGGTCAAGTCATTGAAATTATCTATAAGGGTGAGCACAACCACAAACGACCTCAACCGAACAAACGGGCAAAGGATGTTGGAAATTCGAATGGATGTTCAAGTATTCATGGAAATCCTGAGCTGTCTTCTCAAGTTCAGAGTGGATATTTGAATAAATTAAATGATCAGCAGACGGCTGTGTCTTCCATTGCAAAGAAGGATCAAGAATCGAGTCATGTTACACATGAACAGTTGTCCAGTACTAGTGATGGTGAGGGAGGGAGTGAGATAGAAACTGGAGTGAATCGAAAAGATGAAGACGAACCTGACGCCAAGAGACGGAATACTGAGGTCAGAGTTTCTGAGCCAGCAACTTCTCATAGGACGCTCACAGAATCCAGAATCATTGTGCAGACTACAAGTGAAGTCGATCTATTGGATGATGGCTACAGGTGGCGAAAGTATGGGCAGAAAGTTGTTAAAGGCAACCCTTACCCCAGGAGCTACTATAAATGCACAACTCCGGGATGCAATGTTCGTAAACATGTTGAGAGAGCTTCGACAGACCAAAAAGCTGTCATAACAACGTATGAGGGCAAACACAATCATGATGTTCCAGCTGGTAAAATCAGCAGCCACAGTACAGTCAACAGTAACGTTCCTCAGTTAAAATCACAAAATATAGCTACTGATAACAACACCGATCCTGGGAACTCTCGTCAACAGCCTGTTGGACTTCTACGATTAAAGAAAGAACAAATAACATAG

Protein sequence

MGDDEEQLPPSSAPPPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVSSFFSDSDDCKSFSQLLAGAMASPVAVPPRASELKGPAGLLDSPGLFSPGQGPFEMTHQQALAQVTMQAAQTYSHKQIQGESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVASKESSDISQSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKKVERSLEGQVIEIIYKGEHNHKRPQPNKRAKDVGNSNGCSSIHGNPELSSQVQSGYLNKLNDQQTAVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRVSEPATSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRKHVERASTDQKAVITTYEGKHNHDVPAGKISSHSTVNSNVPQLKSQNIATDNNTDPGNSRQQPVGLLRLKKEQIT
Homology
BLAST of Lag0040147 vs. NCBI nr
Match: XP_008446405.2 (PREDICTED: probable WRKY transcription factor 3 isoform X1 [Cucumis melo] >TYK17683.1 putative WRKY transcription factor 3 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 787.7 bits (2033), Expect = 5.7e-224
Identity = 414/500 (82.80%), Postives = 440/500 (88.00%), Query Frame = 0

Query: 1   MGDDEEQLPPSSAPPPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVS 60
           MG+D+EQ PP   PPPPSKSK   LRPTINLPPRT MESLFSGGPGLG GFSPGPMTLVS
Sbjct: 1   MGEDDEQPPP---PPPPSKSKPFALRPTINLPPRTSMESLFSGGPGLGFGFSPGPMTLVS 60

Query: 61  SFFSDSDDCKSFSQLLAGAMASPV-AVPPRASELKGPAGLLDSPGLFSPGQGPFEMTHQQ 120
           SFFSDSDDCKSFSQLLAGAMASPV AVPP  SE K   GLLDSPGLFSPGQGPF MTHQQ
Sbjct: 61  SFFSDSDDCKSFSQLLAGAMASPVAAVPPSTSEFKSSPGLLDSPGLFSPGQGPFGMTHQQ 120

Query: 121 ALAQVTMQAAQTYSHKQIQGESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVA 180
           ALAQVTMQA + YSH Q+Q  SFSS V PSASS +LLT+ PGEKTKDQ +     NS+VA
Sbjct: 121 ALAQVTMQAVEAYSHNQMQAASFSSSVAPSASSLQLLTSLPGEKTKDQLMQLPFHNSSVA 180

Query: 181 SKESSDISQSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKK 240
           SKE SD SQS+QRLQ SSCNVD+PADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKK
Sbjct: 181 SKEPSDNSQSEQRLQLSSCNVDKPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKK 240

Query: 241 KVERSLEGQVIEIIYKGEHNHKRPQPNKRAKDVGNSNGCSSIHGNPELSSQVQSGYLNKL 300
           KVERSLEGQV EIIYKGEHNHKRPQPNKR+KDVGNSNG S +HGN ELSSQV+SGYLNKL
Sbjct: 241 KVERSLEGQVTEIIYKGEHNHKRPQPNKRSKDVGNSNGYSIVHGNLELSSQVRSGYLNKL 300

Query: 301 NDQQTAVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRV 360
            D++T++SSI KKDQESS VT++Q S  SDGEGGSEIETGVNRKDEDEPDAKRRNTEVR 
Sbjct: 301 -DEETSISSIRKKDQESSRVTNDQFSGNSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRN 360

Query: 361 SEPATSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVR 420
           SEPA+SHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQK+VKGNPYPRSYYKCTTPGCNVR
Sbjct: 361 SEPASSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKIVKGNPYPRSYYKCTTPGCNVR 420

Query: 421 KHVERASTDQKAVITTYEGKHNHDVPAGKISSHSTVNSNVPQLKSQNIATD-----NNTD 480
           KHVERASTD KAVITTYEGKHNHDVP GK SSHS+V+ N+ QLKSQNI T+     NNTD
Sbjct: 421 KHVERASTDPKAVITTYEGKHNHDVPLGKTSSHSSVSGNISQLKSQNIVTEKKNSSNNTD 480

Query: 481 PGNSRQQPVGLLRLKKEQIT 495
            GNS QQP GLLRLK+EQIT
Sbjct: 481 RGNSHQQPTGLLRLKEEQIT 496

BLAST of Lag0040147 vs. NCBI nr
Match: XP_022151251.1 (probable WRKY transcription factor 3 [Momordica charantia])

HSP 1 Score: 781.2 bits (2016), Expect = 5.4e-222
Identity = 421/502 (83.86%), Postives = 445/502 (88.65%), Query Frame = 0

Query: 1   MGDDEEQLPPSSAPPPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVS 60
           MGDDEE+ PP+S   PP KSKA+  RPTINLPPRT MES F GGPG G GFSPGPMTLVS
Sbjct: 1   MGDDEEEQPPNS---PPPKSKAVLSRPTINLPPRTSMESFF-GGPGFGFGFSPGPMTLVS 60

Query: 61  SFFSDSDDCKSFSQLLAGAMASPVAVPPRAS---ELKGPAGLLDSPGLFSPGQGPFEMTH 120
           SFFSDSDDCKSFSQLLAGAMASPVAVPP A    ELKG AG LDS GLFSPGQGPF MTH
Sbjct: 61  SFFSDSDDCKSFSQLLAGAMASPVAVPPHAPTALELKGSAG-LDSAGLFSPGQGPFGMTH 120

Query: 121 QQALAQVTMQAAQTYSHKQIQGESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSA 180
           QQALAQVTMQAAQTYSHKQ++ ESFSS V P A+S+EL  TFPGEKTKDQ + P LLNSA
Sbjct: 121 QQALAQVTMQAAQTYSHKQMEAESFSSSVAP-AASAELSATFPGEKTKDQLMQPPLLNSA 180

Query: 181 VASKESSDISQSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPV 240
           VA+ ES DISQSDQRL  SSCNVDRPA+DGYNWRKYGQKQVKGSEFPRSYYKCTH NCPV
Sbjct: 181 VATTESLDISQSDQRLPPSSCNVDRPANDGYNWRKYGQKQVKGSEFPRSYYKCTHLNCPV 240

Query: 241 KKKVERSLEGQVIEIIYKGEHNHKRPQPNKRAKDVGNSNGCSSIHGNPELSSQVQSGYLN 300
           KKKVERSLEGQV EIIYKGEHNH+RPQ NKRAKDVGNSNG SS  GNPELSSQ+QSGYLN
Sbjct: 241 KKKVERSLEGQVTEIIYKGEHNHERPQLNKRAKDVGNSNGYSSFPGNPELSSQLQSGYLN 300

Query: 301 KLNDQQTAVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEV 360
           K N +QTAVSSIAKKDQ+SS VTHEQLS TSDGEG SEIETGVN+KDEDEPDAKRRNTEV
Sbjct: 301 KSN-EQTAVSSIAKKDQDSSLVTHEQLSGTSDGEGLSEIETGVNQKDEDEPDAKRRNTEV 360

Query: 361 RVSEPATSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCN 420
           RVSEPA+SHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQK+VKGNPYPRSYYKCTTPGCN
Sbjct: 361 RVSEPASSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKIVKGNPYPRSYYKCTTPGCN 420

Query: 421 VRKHVERASTDQKAVITTYEGKHNHDVPAGKISSHSTVNSNVPQLKSQNIATD-----NN 480
           VRKHVERASTD KAV+TTYEGKHNHDVPAG+ +SHST NSNVP LKSQ++ T+      N
Sbjct: 421 VRKHVERASTDPKAVVTTYEGKHNHDVPAGRTTSHSTFNSNVPHLKSQSMVTEKRFSSKN 480

Query: 481 TDPGNSRQQPVGLLRLKKEQIT 495
           TD GNSRQQPVGLLRLK+EQIT
Sbjct: 481 TDSGNSRQQPVGLLRLKEEQIT 495

BLAST of Lag0040147 vs. NCBI nr
Match: KAG7032241.1 (putative WRKY transcription factor 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 768.5 bits (1983), Expect = 3.6e-218
Identity = 406/494 (82.19%), Postives = 425/494 (86.03%), Query Frame = 0

Query: 1   MGDDEEQLPPSSAPPPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVS 60
           MGDDEE+ PPSS P PPSK K  PLRPTINLPPRT MESLFSGGPGLG GFSPGPMTLVS
Sbjct: 1   MGDDEEEPPPSS-PQPPSKLKPRPLRPTINLPPRTSMESLFSGGPGLGFGFSPGPMTLVS 60

Query: 61  SFFSDSDDCKSFSQLLAGAMASPVAVPPRASELKGPAGLLDSPGLFSPGQGPFEMTHQQA 120
           SFFSD DDC+SFSQLLAGAM SPVAVPP  SE KGP GLLDSPGL SP QGPF MTHQQA
Sbjct: 61  SFFSDPDDCRSFSQLLAGAMTSPVAVPPLESEFKGPPGLLDSPGLLSPSQGPFGMTHQQA 120

Query: 121 LAQVTMQAAQTYSHKQIQGESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVAS 180
           LAQVTMQAA+ YSHKQIQ  S S  V P ASS EL TTF GEKTK+QP+ P   N+ VAS
Sbjct: 121 LAQVTMQAAEAYSHKQIQAASLSPSVAPFASSLELSTTFLGEKTKEQPMQPPFPNAGVAS 180

Query: 181 KESSDISQSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKK 240
           KE  DISQS+QR+QSS C V RPADDGYNWRKYGQKQVKG EFPRSYYKCTHPNCPV+KK
Sbjct: 181 KEVLDISQSEQRVQSSLCTVGRPADDGYNWRKYGQKQVKGGEFPRSYYKCTHPNCPVRKK 240

Query: 241 VERSLEGQVIEIIYKGEHNHKRPQPNKRAKDVGNSNGCSSIHGNPELSSQVQSGYLNKLN 300
           VERSLEGQV EIIYKGEHNHK+PQPNKR KDVGNSNG SS HGNPEL SQVQS  LN+L 
Sbjct: 241 VERSLEGQVTEIIYKGEHNHKQPQPNKRGKDVGNSNGYSSTHGNPELCSQVQS-KLNELK 300

Query: 301 DQQTAVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRVS 360
           D      S  K DQESSHVTHEQLS TSDGEGGSEIETGVNRKD+DEPDAKRRNTEVR+ 
Sbjct: 301 D-----VSFIKNDQESSHVTHEQLSGTSDGEGGSEIETGVNRKDDDEPDAKRRNTEVRLP 360

Query: 361 EPATSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRK 420
           EPA+SHRTL ESRIIVQTTSEVDLLDDGYRWRKYGQK+VKGNPYPRSYYKCTTPGCNVRK
Sbjct: 361 EPASSHRTLAESRIIVQTTSEVDLLDDGYRWRKYGQKIVKGNPYPRSYYKCTTPGCNVRK 420

Query: 421 HVERASTDQKAVITTYEGKHNHDVPAGKISSHSTVNSNVPQLKSQNIATDNNTDPGNSRQ 480
           HVERASTD KAVITTYEGKHNHDVPAGKISSH +VNSNVPQLKS NI+  NN D GNSR 
Sbjct: 421 HVERASTDPKAVITTYEGKHNHDVPAGKISSHRSVNSNVPQLKSHNISNKNN-DHGNSRH 480

Query: 481 QPVGLLRLKKEQIT 495
           Q  G+LRLK+EQIT
Sbjct: 481 QHAGVLRLKEEQIT 486

BLAST of Lag0040147 vs. NCBI nr
Match: XP_023515298.1 (probable WRKY transcription factor 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 765.4 bits (1975), Expect = 3.0e-217
Identity = 405/494 (81.98%), Postives = 422/494 (85.43%), Query Frame = 0

Query: 1   MGDDEEQLPPSSAPPPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVS 60
           MGDDEE+ PPSS P PPSK K  PLRPTINLPPRT MESLFSGGPGLG GFSPGPMTLVS
Sbjct: 1   MGDDEEEPPPSS-PQPPSKLKLRPLRPTINLPPRTSMESLFSGGPGLGFGFSPGPMTLVS 60

Query: 61  SFFSDSDDCKSFSQLLAGAMASPVAVPPRASELKGPAGLLDSPGLFSPGQGPFEMTHQQA 120
           SFFSD DDCKSFSQLLAGAM SPVA PP  SE KGP GLLDSPGL SP QGPF MTHQQA
Sbjct: 61  SFFSDPDDCKSFSQLLAGAMTSPVAAPPLESEFKGPPGLLDSPGLLSPSQGPFGMTHQQA 120

Query: 121 LAQVTMQAAQTYSHKQIQGESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVAS 180
           LAQVTMQAA+ YSHKQIQ  S S  V P ASS EL TTF GEKTKD P+ P   N+ VAS
Sbjct: 121 LAQVTMQAAEAYSHKQIQAASLSPSVAPFASSLELSTTFLGEKTKDHPMQPPFPNAGVAS 180

Query: 181 KESSDISQSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKK 240
           KE  DISQS+QR+QSS C V RPADDGYNWRKYGQKQVKG EFPRSYYKCTHPNCPV+KK
Sbjct: 181 KEVLDISQSEQRVQSSLCTVGRPADDGYNWRKYGQKQVKGGEFPRSYYKCTHPNCPVRKK 240

Query: 241 VERSLEGQVIEIIYKGEHNHKRPQPNKRAKDVGNSNGCSSIHGNPELSSQVQSGYLNKLN 300
           VERSLEGQV EIIYKGEHNHK+PQPNKR KDV NSNGCSS HGNPEL SQVQ   LN+L 
Sbjct: 241 VERSLEGQVTEIIYKGEHNHKQPQPNKRGKDVVNSNGCSSTHGNPELCSQVQR-KLNELK 300

Query: 301 DQQTAVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRVS 360
           D      S  K DQESSHVTHEQLS TSDGEGGSEIETGVNRKD+DEPDAKRRNTEVR+S
Sbjct: 301 D-----VSFIKNDQESSHVTHEQLSGTSDGEGGSEIETGVNRKDDDEPDAKRRNTEVRLS 360

Query: 361 EPATSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRK 420
           EPA+SHRTL ESRIIVQTTSEVDLLDDGYRWRKYGQK+VKGNPYPRSYYKCTT GCNVRK
Sbjct: 361 EPASSHRTLAESRIIVQTTSEVDLLDDGYRWRKYGQKIVKGNPYPRSYYKCTTSGCNVRK 420

Query: 421 HVERASTDQKAVITTYEGKHNHDVPAGKISSHSTVNSNVPQLKSQNIATDNNTDPGNSRQ 480
           HVERASTD KAVITTYEGKHNHDVPAGKISSH +VNSNVPQLKS NI+  NN D GNSR 
Sbjct: 421 HVERASTDPKAVITTYEGKHNHDVPAGKISSHRSVNSNVPQLKSHNISNKNN-DHGNSRH 480

Query: 481 QPVGLLRLKKEQIT 495
           Q  G+LRLK+EQIT
Sbjct: 481 QHAGVLRLKEEQIT 486

BLAST of Lag0040147 vs. NCBI nr
Match: XP_022957438.1 (probable WRKY transcription factor 4 [Cucurbita moschata])

HSP 1 Score: 761.9 bits (1966), Expect = 3.4e-216
Identity = 406/494 (82.19%), Postives = 425/494 (86.03%), Query Frame = 0

Query: 1   MGDDEEQLPPSSAPPPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVS 60
           MGDDEE+ PPSS P PPSK K  PLRPTINLPPRT MESLFSGG GLG GFSPGPMTLVS
Sbjct: 1   MGDDEEEPPPSS-PQPPSKLKPRPLRPTINLPPRTSMESLFSGGLGLGFGFSPGPMTLVS 60

Query: 61  SFFSDSDDCKSFSQLLAGAMASPVAVPPRASELKGPAGLLDSPGLFSPGQGPFEMTHQQA 120
           SFFSD DDCKSFSQLLAGAM SPVAVPP  SE KGP GLLDSPGL SP QGPF MTHQQA
Sbjct: 61  SFFSDPDDCKSFSQLLAGAMTSPVAVPPLESEFKGPPGLLDSPGLLSPSQGPFGMTHQQA 120

Query: 121 LAQVTMQAAQTYSHKQIQGESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVAS 180
           LAQVTMQAA+ YSHKQIQ  S S  V P ASS EL TTF GEKTK+QP+ P   N+ VAS
Sbjct: 121 LAQVTMQAAEAYSHKQIQAASLSPSVAPFASSLELSTTFLGEKTKEQPMQPPFPNAGVAS 180

Query: 181 KESSDISQSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKK 240
           KE  DISQS+QR+QSS C V RP DDGYNWRKYGQKQVKG EFPRSYYKCTHPNCPV+KK
Sbjct: 181 KEVLDISQSEQRVQSSLCTVGRPGDDGYNWRKYGQKQVKGGEFPRSYYKCTHPNCPVRKK 240

Query: 241 VERSLEGQVIEIIYKGEHNHKRPQPNKRAKDVGNSNGCSSIHGNPELSSQVQSGYLNKLN 300
           VERSLEGQV EIIYKGEHNHK+PQPNKR KDVGNSNG SS HGNPEL SQVQS  LN+L 
Sbjct: 241 VERSLEGQVTEIIYKGEHNHKQPQPNKRGKDVGNSNGYSSTHGNPELCSQVQS-KLNELK 300

Query: 301 DQQTAVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRVS 360
           D    VSSI K DQESSHVTHE+LS TSDGEGGSEIETGVNRKD+DEPDAKRRNTEVR+ 
Sbjct: 301 D----VSSI-KNDQESSHVTHEKLSGTSDGEGGSEIETGVNRKDDDEPDAKRRNTEVRLP 360

Query: 361 EPATSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRK 420
           EPA+SHRTL ESRIIVQTTSEVDLLDDGYRWRKYGQK+VKGNPYPRSYYKCTTPGCNVRK
Sbjct: 361 EPASSHRTLAESRIIVQTTSEVDLLDDGYRWRKYGQKIVKGNPYPRSYYKCTTPGCNVRK 420

Query: 421 HVERASTDQKAVITTYEGKHNHDVPAGKISSHSTVNSNVPQLKSQNIATDNNTDPGNSRQ 480
           HVERAS D KAVITTYEGKHNHDVPAGKISSH +VNSNVPQLKS NI+  NN D GNSR 
Sbjct: 421 HVERASMDPKAVITTYEGKHNHDVPAGKISSHRSVNSNVPQLKSHNISNKNN-DHGNSRH 480

Query: 481 QPVGLLRLKKEQIT 495
           Q  G+LRLK+EQIT
Sbjct: 481 QHAGVLRLKEEQIT 486

BLAST of Lag0040147 vs. ExPASy Swiss-Prot
Match: Q9XI90 (Probable WRKY transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=WRKY4 PE=1 SV=2)

HSP 1 Score: 442.2 bits (1136), Expect = 7.7e-123
Identity = 282/538 (52.42%), Postives = 330/538 (61.34%), Query Frame = 0

Query: 15  PPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVSSFFSDSDDCKSFSQ 74
           P  SKS   P RPT++LPPR   E  F+G    G GFSPGPMTLVS+ F DSD+ +SFSQ
Sbjct: 8   PSTSKSTGAPSRPTLSLPPRPFSEMFFNG----GVGFSPGPMTLVSNMFPDSDEFRSFSQ 67

Query: 75  LLAGAMASPVA--------------------------VPPRASELK-------------- 134
           LLAGAM+SP                            V PR  + +              
Sbjct: 68  LLAGAMSSPATAAAAAAAATASDYQRLGEGTNSSSGDVDPRFKQNRPTGLMISQSQSPSM 127

Query: 135 -------GPAGLLDSP---GLFSPGQGPFEMTHQQALAQVTMQAAQTYSHKQIQGESFSS 194
                   PA LLDSP   GLFSP QG + MTHQQALAQVT QA Q  ++ Q Q E    
Sbjct: 128 FTVPPGLSPAMLLDSPSFLGLFSPVQGSYGMTHQQALAQVTAQAVQANANMQPQTEY--- 187

Query: 195 LVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVASKESSDISQSDQRLQSSSCNVDRPA 254
              P  S  +  ++   +     P+P        A +E+SD++  + R Q    NVD+PA
Sbjct: 188 ---PPPSQVQSFSSGQAQIPTSAPLP--------AQRETSDVTIIEHRSQ-QPLNVDKPA 247

Query: 255 DDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKKVERSLEGQVIEIIYKGEHNHKRPQ 314
           DDGYNWRKYGQKQVKGSEFPRSYYKCT+P CPVKKKVERSL+GQV EIIYKG+HNH+ PQ
Sbjct: 248 DDGYNWRKYGQKQVKGSEFPRSYYKCTNPGCPVKKKVERSLDGQVTEIIYKGQHNHEPPQ 307

Query: 315 PNKRAK--DVGNSNGCS--SIHGNPEL-SSQVQSGYLNKLNDQQTAVSSIAKKDQESSHV 374
             KR    +  N NG S  +  G+ EL +SQ Q+   NK   +Q         +  S   
Sbjct: 308 NTKRGNKDNTANINGSSINNNRGSSELGASQFQTNSSNKTKREQ--------HEAVSQAT 367

Query: 375 THEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRVSE--PATSHRTLTESRIIVQ 434
           T E LS  SDGE     ET V  KDE+EPD KRR+TEVR+SE  PA SHRT+TE RIIVQ
Sbjct: 368 TTEHLSEASDGEEVGNGETDVREKDENEPDPKRRSTEVRISEPAPAASHRTVTEPRIIVQ 427

Query: 435 TTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRKHVERASTDQKAVITTYE 494
           TTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGC VRKHVERA+TD KAV+TTYE
Sbjct: 428 TTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCGVRKHVERAATDPKAVVTTYE 487

BLAST of Lag0040147 vs. ExPASy Swiss-Prot
Match: Q9ZQ70 (Probable WRKY transcription factor 3 OS=Arabidopsis thaliana OX=3702 GN=WRKY3 PE=2 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.5e-121
Identity = 292/542 (53.87%), Postives = 331/542 (61.07%), Query Frame = 0

Query: 15  PPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVSSFFSDSDDCKSFSQ 74
           P   KS     RPTI+LPPR   E  FSG    G GFSPGPMTLVS+ FSD D+ KSFSQ
Sbjct: 9   PSKLKSSTGVSRPTISLPPRPFGEMFFSG----GVGFSPGPMTLVSNLFSDPDEFKSFSQ 68

Query: 75  LLAGAMASPVA------------------------------VPPRASELK---------- 134
           LLAGAMASP A                              V PR  + +          
Sbjct: 69  LLAGAMASPAAAAVAAAAVVATAHHQTPVSSVGDGGGSGGDVDPRFKQSRPTGLMITQPP 128

Query: 135 ---------GPAGLLDSP---GLFSPGQGPFEMTHQQALAQVTMQAAQ---TYSHKQIQG 194
                     PA LLDSP   GLFSP QG F MTHQQALAQVT QA Q    +  +  Q 
Sbjct: 129 GMFTVPPGLSPATLLDSPSFFGLFSPLQGTFGMTHQQALAQVTAQAVQGNNVHMQQSQQS 188

Query: 195 ESFSSLVPPSASSSEL-LTTFPG-EKTKDQPVPPSLLNSAVASKESSDISQSDQRLQSSS 254
           E  SS         +  LT  P         +  S+  ++   +E+S+IS  + R Q   
Sbjct: 189 EYPSSTQQQQQQQQQASLTEIPSFSSAPRSQIRASVQETSQGQRETSEISVFEHRSQPQ- 248

Query: 255 CNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKKVERSLEGQVIEIIYKGE 314
            N D+PADDGYNWRKYGQKQVKGS+FPRSYYKCTHP CPVKKKVERSL+GQV EIIYKG+
Sbjct: 249 -NADKPADDGYNWRKYGQKQVKGSDFPRSYYKCTHPACPVKKKVERSLDGQVTEIIYKGQ 308

Query: 315 HNHKRPQPNKRAKDVGNSNGCSSIHGNPELSSQVQSGYLNKLNDQQTAVSSI--AKKDQE 374
           HNH+ PQ  KR    GN+NG          SS +        N  QT+ SS+  +K+DQE
Sbjct: 309 HNHELPQ--KR----GNNNGSCK-------SSDI-------ANQFQTSNSSLNKSKRDQE 368

Query: 375 SSHV-THEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRVSEP-ATSHRTLTESR 434
           +S V T EQ+S  SD E     ET V  + EDEPD KRRNTEVRVSEP A+SHRT+TE R
Sbjct: 369 TSQVTTTEQMSEASDSEEVGNAETSVGERHEDEPDPKRRNTEVRVSEPVASSHRTVTEPR 428

Query: 435 IIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRKHVERASTDQKAVI 494
           IIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTP C VRKHVERA+TD KAV+
Sbjct: 429 IIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPDCGVRKHVERAATDPKAVV 488

BLAST of Lag0040147 vs. ExPASy Swiss-Prot
Match: Q93WU7 (Probable WRKY transcription factor 58 OS=Arabidopsis thaliana OX=3702 GN=WRKY58 PE=2 SV=2)

HSP 1 Score: 289.7 bits (740), Expect = 6.4e-77
Identity = 209/502 (41.63%), Postives = 260/502 (51.79%), Query Frame = 0

Query: 24  PLRPTINLPPR-TCMES---LFSGGPGLGSGFSPGPMTLVSSFFSDSDDCKSFSQLLAGA 83
           P RPTI +P R   +E+    F GG GL    SPGP++ VSS F D+             
Sbjct: 18  PTRPTITVPHRPPAIETAAYFFGGGDGL--SLSPGPLSFVSSLFVDN------------- 77

Query: 84  MASPVAVPPRASELKGPAGLLDSPGLFSPGQGPFEMTHQQALAQVTMQAAQTYSHKQIQG 143
              P  + P          LL SP  F P         Q +     +Q  Q    +  + 
Sbjct: 78  --FPDVLTPDNQRTTSFTHLLTSPMFFPP---------QSSAHTGFIQPRQQSQPQPQRP 137

Query: 144 ESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVASKESSDISQSDQRLQS---- 203
           ++F   +PPS S                         AV  ++S D+SQ DQR ++    
Sbjct: 138 DTFPHHMPPSTS------------------------VAVHGRQSLDVSQVDQRARNHYNN 197

Query: 204 ----------SSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKKVERSL 263
                     +  NVD+PADDGYNWRKYGQK +KG E+PRSYYKCTH NCPVKKKVERS 
Sbjct: 198 PGNNNNNRSYNVVNVDKPADDGYNWRKYGQKPIKGCEYPRSYYKCTHVNCPVKKKVERSS 257

Query: 264 EGQVIEIIYKGEHNHKRPQPNKRAKDVGNSNGCSSIHGNPE-LSSQVQSGYLNKLNDQQT 323
           +GQ+ +IIYKG+H+H+RPQ N+R    G     + + G  + + S   SGY         
Sbjct: 258 DGQITQIIYKGQHDHERPQ-NRRG---GGGRDSTEVGGAGQMMESSDDSGY--------- 317

Query: 324 AVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRVSEPAT 383
                 +KD +      E                     DED P +K R    R+   +T
Sbjct: 318 ------RKDHDDDDDDDED--------------------DEDLPASKIR----RIDGVST 377

Query: 384 SHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRKHVER 443
           +HRT+TE +IIVQT SEVDLLDDGYRWRKYGQKVVKGNP+PRSYYKCTTP C VRKHVER
Sbjct: 378 THRTVTEPKIIVQTKSEVDLLDDGYRWRKYGQKVVKGNPHPRSYYKCTTPNCTVRKHVER 422

Query: 444 ASTDQKAVITTYEGKHNHDVPAGK------------ISSHSTVNSNVPQLKSQNIATDNN 495
           ASTD KAVITTYEGKHNHDVPA +             S H  + S       Q+++  NN
Sbjct: 438 ASTDAKAVITTYEGKHNHDVPAARNGTAAATAAAVGPSDHHRMRSMSGNNMQQHMSFGNN 422

BLAST of Lag0040147 vs. ExPASy Swiss-Prot
Match: Q9FG77 (Probable WRKY transcription factor 2 OS=Arabidopsis thaliana OX=3702 GN=WRKY2 PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 5.5e-68
Identity = 156/336 (46.43%), Postives = 194/336 (57.74%), Query Frame = 0

Query: 188 QSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKKVERSLEG 247
           + +++ +  S     PA+DGYNWRKYGQK VKGSE+PRSYYKCT+PNC VKKKVERS EG
Sbjct: 256 EEEEQRRGDSMAGGAPAEDGYNWRKYGQKLVKGSEYPRSYYKCTNPNCQVKKKVERSREG 315

Query: 248 QVIEIIYKGEHNHKRPQPNKRA-----------------------------KDVGNSN-- 307
            + EIIYKG HNH +P PN+R+                             +  G SN  
Sbjct: 316 HITEIIYKGAHNHLKPPPNRRSGMQVDGTEQVEQQQQQRDSAATWVSCNNTQQQGGSNEN 375

Query: 308 ----GCSSI-HGNPELSSQVQSGYLNKLNDQQTAV---SSIAKKDQESSHVTHEQLSSTS 367
               G +   +GN   S Q Q+G   +  D    V   S+ +  + E    TH  +S   
Sbjct: 376 NVEEGSTRFEYGNQSGSIQAQTGGQYESGDPVVVVDASSTFSNDEDEDDRGTHGSVSLGY 435

Query: 368 DGEGGSEIETGVNRKDEDEPDAKRRNTEVRVSEPATSHRTLTESRIIVQTTSEVDLLDDG 427
           DG GG     G      DE ++KRR  E   +E + S R + E R++VQTTS+VD+LDDG
Sbjct: 436 DGGGGGGGGEG------DESESKRRKLEAFAAEMSGSTRAIREPRVVVQTTSDVDILDDG 495

Query: 428 YRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRKHVERASTDQKAVITTYEGKHNHDVPAGK 485
           YRWRKYGQKVVKGNP PRSYYKCT PGC VRKHVERAS D K+VITTYEGKHNHDVPA +
Sbjct: 496 YRWRKYGQKVVKGNPNPRSYYKCTAPGCTVRKHVERASHDLKSVITTYEGKHNHDVPAAR 555

BLAST of Lag0040147 vs. ExPASy Swiss-Prot
Match: Q8S8P5 (Probable WRKY transcription factor 33 OS=Arabidopsis thaliana OX=3702 GN=WRKY33 PE=1 SV=2)

HSP 1 Score: 252.3 bits (643), Expect = 1.1e-65
Identity = 176/392 (44.90%), Postives = 217/392 (55.36%), Query Frame = 0

Query: 82  SPVAVPPRASELKGPAGLLDSPGLFSPGQGPFEMTHQQAL-AQVTMQAAQTYSHKQIQG- 141
           SP  V P  S    P+  LDSP   S            AL   VT Q       K     
Sbjct: 54  SPSLVSP--STCFSPSLFLDSPAFVSSSANVLASPTTGALITNVTNQKGINEGDKSNNNN 113

Query: 142 -ESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVASK--ESSDISQSDQRLQSS 201
              F       +S     TT     T       S+  S    K  +S   SQ++ R  + 
Sbjct: 114 FNLFDFSFHTQSSGVSAPTTTTTTTTTTTTTNSSIFQSQEQQKKNQSEQWSQTETRPNNQ 173

Query: 202 SCNVD----RPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKKVERSLEGQVIEI 261
           + + +    R  +DGYNWRKYGQKQVKGSE PRSYYKCT PNCP KKKVERSLEGQ+ EI
Sbjct: 174 AVSYNGREQRKGEDGYNWRKYGQKQVKGSENPRSYYKCTFPNCPTKKKVERSLEGQITEI 233

Query: 262 IYKGEHNHKRPQPNKRAKDVGNSNGCSSIHG---NPELSSQVQSGYLNKLNDQQTAVSSI 321
           +YKG HNH +PQ  +R     +S+  S+ H    N  L    Q+      +DQ  + +S 
Sbjct: 234 VYKGSHNHPKPQSTRR-----SSSSSSTFHSAVYNASLDHNRQAS-----SDQPNSNNSF 293

Query: 322 AKKDQESSHVTHEQLSSTSDGEGGSEIETG---VNRKDED---EPDAKR-RNTEVRVSEP 381
            + D   S    ++ ++TSD  G  E E G   V+R +ED   EP+AKR +         
Sbjct: 294 HQSD---SFGMQQEDNTTSDSVGDDEFEQGSSIVSRDEEDCGSEPEAKRWKGDNETNGGN 353

Query: 382 ATSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRKHV 441
               +T+ E RI+VQTTS++D+LDDGYRWRKYGQKVVKGNP PRSYYKCTT GC VRKHV
Sbjct: 354 GGGSKTVREPRIVVQTTSDIDILDDGYRWRKYGQKVVKGNPNPRSYYKCTTIGCPVRKHV 413

Query: 442 ERASTDQKAVITTYEGKHNHDVPAGKISSHST 455
           ERAS D +AVITTYEGKHNHDVPA + S ++T
Sbjct: 414 ERASHDMRAVITTYEGKHNHDVPAARGSGYAT 430

BLAST of Lag0040147 vs. ExPASy TrEMBL
Match: A0A5D3D0G9 (Putative WRKY transcription factor 3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1932G00080 PE=4 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.8e-224
Identity = 414/500 (82.80%), Postives = 440/500 (88.00%), Query Frame = 0

Query: 1   MGDDEEQLPPSSAPPPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVS 60
           MG+D+EQ PP   PPPPSKSK   LRPTINLPPRT MESLFSGGPGLG GFSPGPMTLVS
Sbjct: 1   MGEDDEQPPP---PPPPSKSKPFALRPTINLPPRTSMESLFSGGPGLGFGFSPGPMTLVS 60

Query: 61  SFFSDSDDCKSFSQLLAGAMASPV-AVPPRASELKGPAGLLDSPGLFSPGQGPFEMTHQQ 120
           SFFSDSDDCKSFSQLLAGAMASPV AVPP  SE K   GLLDSPGLFSPGQGPF MTHQQ
Sbjct: 61  SFFSDSDDCKSFSQLLAGAMASPVAAVPPSTSEFKSSPGLLDSPGLFSPGQGPFGMTHQQ 120

Query: 121 ALAQVTMQAAQTYSHKQIQGESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVA 180
           ALAQVTMQA + YSH Q+Q  SFSS V PSASS +LLT+ PGEKTKDQ +     NS+VA
Sbjct: 121 ALAQVTMQAVEAYSHNQMQAASFSSSVAPSASSLQLLTSLPGEKTKDQLMQLPFHNSSVA 180

Query: 181 SKESSDISQSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKK 240
           SKE SD SQS+QRLQ SSCNVD+PADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKK
Sbjct: 181 SKEPSDNSQSEQRLQLSSCNVDKPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKK 240

Query: 241 KVERSLEGQVIEIIYKGEHNHKRPQPNKRAKDVGNSNGCSSIHGNPELSSQVQSGYLNKL 300
           KVERSLEGQV EIIYKGEHNHKRPQPNKR+KDVGNSNG S +HGN ELSSQV+SGYLNKL
Sbjct: 241 KVERSLEGQVTEIIYKGEHNHKRPQPNKRSKDVGNSNGYSIVHGNLELSSQVRSGYLNKL 300

Query: 301 NDQQTAVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRV 360
            D++T++SSI KKDQESS VT++Q S  SDGEGGSEIETGVNRKDEDEPDAKRRNTEVR 
Sbjct: 301 -DEETSISSIRKKDQESSRVTNDQFSGNSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRN 360

Query: 361 SEPATSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVR 420
           SEPA+SHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQK+VKGNPYPRSYYKCTTPGCNVR
Sbjct: 361 SEPASSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKIVKGNPYPRSYYKCTTPGCNVR 420

Query: 421 KHVERASTDQKAVITTYEGKHNHDVPAGKISSHSTVNSNVPQLKSQNIATD-----NNTD 480
           KHVERASTD KAVITTYEGKHNHDVP GK SSHS+V+ N+ QLKSQNI T+     NNTD
Sbjct: 421 KHVERASTDPKAVITTYEGKHNHDVPLGKTSSHSSVSGNISQLKSQNIVTEKKNSSNNTD 480

Query: 481 PGNSRQQPVGLLRLKKEQIT 495
            GNS QQP GLLRLK+EQIT
Sbjct: 481 RGNSHQQPTGLLRLKEEQIT 496

BLAST of Lag0040147 vs. ExPASy TrEMBL
Match: A0A1S3BEZ9 (probable WRKY transcription factor 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489160 PE=4 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.8e-224
Identity = 414/500 (82.80%), Postives = 440/500 (88.00%), Query Frame = 0

Query: 1   MGDDEEQLPPSSAPPPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVS 60
           MG+D+EQ PP   PPPPSKSK   LRPTINLPPRT MESLFSGGPGLG GFSPGPMTLVS
Sbjct: 1   MGEDDEQPPP---PPPPSKSKPFALRPTINLPPRTSMESLFSGGPGLGFGFSPGPMTLVS 60

Query: 61  SFFSDSDDCKSFSQLLAGAMASPV-AVPPRASELKGPAGLLDSPGLFSPGQGPFEMTHQQ 120
           SFFSDSDDCKSFSQLLAGAMASPV AVPP  SE K   GLLDSPGLFSPGQGPF MTHQQ
Sbjct: 61  SFFSDSDDCKSFSQLLAGAMASPVAAVPPSTSEFKSSPGLLDSPGLFSPGQGPFGMTHQQ 120

Query: 121 ALAQVTMQAAQTYSHKQIQGESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVA 180
           ALAQVTMQA + YSH Q+Q  SFSS V PSASS +LLT+ PGEKTKDQ +     NS+VA
Sbjct: 121 ALAQVTMQAVEAYSHNQMQAASFSSSVAPSASSLQLLTSLPGEKTKDQLMQLPFHNSSVA 180

Query: 181 SKESSDISQSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKK 240
           SKE SD SQS+QRLQ SSCNVD+PADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKK
Sbjct: 181 SKEPSDNSQSEQRLQLSSCNVDKPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKK 240

Query: 241 KVERSLEGQVIEIIYKGEHNHKRPQPNKRAKDVGNSNGCSSIHGNPELSSQVQSGYLNKL 300
           KVERSLEGQV EIIYKGEHNHKRPQPNKR+KDVGNSNG S +HGN ELSSQV+SGYLNKL
Sbjct: 241 KVERSLEGQVTEIIYKGEHNHKRPQPNKRSKDVGNSNGYSIVHGNLELSSQVRSGYLNKL 300

Query: 301 NDQQTAVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRV 360
            D++T++SSI KKDQESS VT++Q S  SDGEGGSEIETGVNRKDEDEPDAKRRNTEVR 
Sbjct: 301 -DEETSISSIRKKDQESSRVTNDQFSGNSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRN 360

Query: 361 SEPATSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVR 420
           SEPA+SHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQK+VKGNPYPRSYYKCTTPGCNVR
Sbjct: 361 SEPASSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKIVKGNPYPRSYYKCTTPGCNVR 420

Query: 421 KHVERASTDQKAVITTYEGKHNHDVPAGKISSHSTVNSNVPQLKSQNIATD-----NNTD 480
           KHVERASTD KAVITTYEGKHNHDVP GK SSHS+V+ N+ QLKSQNI T+     NNTD
Sbjct: 421 KHVERASTDPKAVITTYEGKHNHDVPLGKTSSHSSVSGNISQLKSQNIVTEKKNSSNNTD 480

Query: 481 PGNSRQQPVGLLRLKKEQIT 495
            GNS QQP GLLRLK+EQIT
Sbjct: 481 RGNSHQQPTGLLRLKEEQIT 496

BLAST of Lag0040147 vs. ExPASy TrEMBL
Match: A0A6J1DE24 (probable WRKY transcription factor 3 OS=Momordica charantia OX=3673 GN=LOC111019219 PE=4 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 2.6e-222
Identity = 421/502 (83.86%), Postives = 445/502 (88.65%), Query Frame = 0

Query: 1   MGDDEEQLPPSSAPPPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVS 60
           MGDDEE+ PP+S   PP KSKA+  RPTINLPPRT MES F GGPG G GFSPGPMTLVS
Sbjct: 1   MGDDEEEQPPNS---PPPKSKAVLSRPTINLPPRTSMESFF-GGPGFGFGFSPGPMTLVS 60

Query: 61  SFFSDSDDCKSFSQLLAGAMASPVAVPPRAS---ELKGPAGLLDSPGLFSPGQGPFEMTH 120
           SFFSDSDDCKSFSQLLAGAMASPVAVPP A    ELKG AG LDS GLFSPGQGPF MTH
Sbjct: 61  SFFSDSDDCKSFSQLLAGAMASPVAVPPHAPTALELKGSAG-LDSAGLFSPGQGPFGMTH 120

Query: 121 QQALAQVTMQAAQTYSHKQIQGESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSA 180
           QQALAQVTMQAAQTYSHKQ++ ESFSS V P A+S+EL  TFPGEKTKDQ + P LLNSA
Sbjct: 121 QQALAQVTMQAAQTYSHKQMEAESFSSSVAP-AASAELSATFPGEKTKDQLMQPPLLNSA 180

Query: 181 VASKESSDISQSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPV 240
           VA+ ES DISQSDQRL  SSCNVDRPA+DGYNWRKYGQKQVKGSEFPRSYYKCTH NCPV
Sbjct: 181 VATTESLDISQSDQRLPPSSCNVDRPANDGYNWRKYGQKQVKGSEFPRSYYKCTHLNCPV 240

Query: 241 KKKVERSLEGQVIEIIYKGEHNHKRPQPNKRAKDVGNSNGCSSIHGNPELSSQVQSGYLN 300
           KKKVERSLEGQV EIIYKGEHNH+RPQ NKRAKDVGNSNG SS  GNPELSSQ+QSGYLN
Sbjct: 241 KKKVERSLEGQVTEIIYKGEHNHERPQLNKRAKDVGNSNGYSSFPGNPELSSQLQSGYLN 300

Query: 301 KLNDQQTAVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEV 360
           K N +QTAVSSIAKKDQ+SS VTHEQLS TSDGEG SEIETGVN+KDEDEPDAKRRNTEV
Sbjct: 301 KSN-EQTAVSSIAKKDQDSSLVTHEQLSGTSDGEGLSEIETGVNQKDEDEPDAKRRNTEV 360

Query: 361 RVSEPATSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCN 420
           RVSEPA+SHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQK+VKGNPYPRSYYKCTTPGCN
Sbjct: 361 RVSEPASSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKIVKGNPYPRSYYKCTTPGCN 420

Query: 421 VRKHVERASTDQKAVITTYEGKHNHDVPAGKISSHSTVNSNVPQLKSQNIATD-----NN 480
           VRKHVERASTD KAV+TTYEGKHNHDVPAG+ +SHST NSNVP LKSQ++ T+      N
Sbjct: 421 VRKHVERASTDPKAVVTTYEGKHNHDVPAGRTTSHSTFNSNVPHLKSQSMVTEKRFSSKN 480

Query: 481 TDPGNSRQQPVGLLRLKKEQIT 495
           TD GNSRQQPVGLLRLK+EQIT
Sbjct: 481 TDSGNSRQQPVGLLRLKEEQIT 495

BLAST of Lag0040147 vs. ExPASy TrEMBL
Match: A0A6J1H078 (probable WRKY transcription factor 4 OS=Cucurbita moschata OX=3662 GN=LOC111458834 PE=4 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 1.6e-216
Identity = 406/494 (82.19%), Postives = 425/494 (86.03%), Query Frame = 0

Query: 1   MGDDEEQLPPSSAPPPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVS 60
           MGDDEE+ PPSS P PPSK K  PLRPTINLPPRT MESLFSGG GLG GFSPGPMTLVS
Sbjct: 1   MGDDEEEPPPSS-PQPPSKLKPRPLRPTINLPPRTSMESLFSGGLGLGFGFSPGPMTLVS 60

Query: 61  SFFSDSDDCKSFSQLLAGAMASPVAVPPRASELKGPAGLLDSPGLFSPGQGPFEMTHQQA 120
           SFFSD DDCKSFSQLLAGAM SPVAVPP  SE KGP GLLDSPGL SP QGPF MTHQQA
Sbjct: 61  SFFSDPDDCKSFSQLLAGAMTSPVAVPPLESEFKGPPGLLDSPGLLSPSQGPFGMTHQQA 120

Query: 121 LAQVTMQAAQTYSHKQIQGESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVAS 180
           LAQVTMQAA+ YSHKQIQ  S S  V P ASS EL TTF GEKTK+QP+ P   N+ VAS
Sbjct: 121 LAQVTMQAAEAYSHKQIQAASLSPSVAPFASSLELSTTFLGEKTKEQPMQPPFPNAGVAS 180

Query: 181 KESSDISQSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKK 240
           KE  DISQS+QR+QSS C V RP DDGYNWRKYGQKQVKG EFPRSYYKCTHPNCPV+KK
Sbjct: 181 KEVLDISQSEQRVQSSLCTVGRPGDDGYNWRKYGQKQVKGGEFPRSYYKCTHPNCPVRKK 240

Query: 241 VERSLEGQVIEIIYKGEHNHKRPQPNKRAKDVGNSNGCSSIHGNPELSSQVQSGYLNKLN 300
           VERSLEGQV EIIYKGEHNHK+PQPNKR KDVGNSNG SS HGNPEL SQVQS  LN+L 
Sbjct: 241 VERSLEGQVTEIIYKGEHNHKQPQPNKRGKDVGNSNGYSSTHGNPELCSQVQS-KLNELK 300

Query: 301 DQQTAVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRVS 360
           D    VSSI K DQESSHVTHE+LS TSDGEGGSEIETGVNRKD+DEPDAKRRNTEVR+ 
Sbjct: 301 D----VSSI-KNDQESSHVTHEKLSGTSDGEGGSEIETGVNRKDDDEPDAKRRNTEVRLP 360

Query: 361 EPATSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRK 420
           EPA+SHRTL ESRIIVQTTSEVDLLDDGYRWRKYGQK+VKGNPYPRSYYKCTTPGCNVRK
Sbjct: 361 EPASSHRTLAESRIIVQTTSEVDLLDDGYRWRKYGQKIVKGNPYPRSYYKCTTPGCNVRK 420

Query: 421 HVERASTDQKAVITTYEGKHNHDVPAGKISSHSTVNSNVPQLKSQNIATDNNTDPGNSRQ 480
           HVERAS D KAVITTYEGKHNHDVPAGKISSH +VNSNVPQLKS NI+  NN D GNSR 
Sbjct: 421 HVERASMDPKAVITTYEGKHNHDVPAGKISSHRSVNSNVPQLKSHNISNKNN-DHGNSRH 480

Query: 481 QPVGLLRLKKEQIT 495
           Q  G+LRLK+EQIT
Sbjct: 481 QHAGVLRLKEEQIT 486

BLAST of Lag0040147 vs. ExPASy TrEMBL
Match: A0A0A0KW35 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G606470 PE=4 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 2.6e-214
Identity = 405/500 (81.00%), Postives = 429/500 (85.80%), Query Frame = 0

Query: 1   MGDDEEQLPPSSAPPPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVS 60
           MG+D+EQ PP   PP PSKSK+ PLRPTINLPPRT MESLFS GPGLG GFSPGPMTLVS
Sbjct: 1   MGEDDEQPPP---PPLPSKSKSFPLRPTINLPPRTSMESLFSSGPGLGFGFSPGPMTLVS 60

Query: 61  SFFSDSDDCKSFSQLLAGAMASPV-AVPPRASELKGPAGLLDSPGLFSPGQGPFEMTHQQ 120
           SFFSDSDDCKSFSQLLAGAMASPV AVP  +SE K   GLLDSPGL    QGPF MTHQQ
Sbjct: 61  SFFSDSDDCKSFSQLLAGAMASPVTAVPSSSSEFKASPGLLDSPGL----QGPFGMTHQQ 120

Query: 121 ALAQVTMQAAQTYSHKQIQGESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVA 180
           ALAQVTM A + YS  Q+Q  SFSS V PSASS +LLT+ PGEKT DQ +     NS VA
Sbjct: 121 ALAQVTMHAVEAYSLNQMQASSFSSSVAPSASSLQLLTSLPGEKTNDQLMQLPFQNSTVA 180

Query: 181 SKESSDISQSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKK 240
           SKE S+ SQS+QRLQ SSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKK
Sbjct: 181 SKEISENSQSEQRLQLSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKK 240

Query: 241 KVERSLEGQVIEIIYKGEHNHKRPQPNKRAKDVGNSNGCSSIHGNPELSSQVQSGYLNKL 300
           KVERSLEGQV EIIYKGEHNHKRPQPNKRAKDVGNSNG S IHGN ELSSQVQSGYLNKL
Sbjct: 241 KVERSLEGQVTEIIYKGEHNHKRPQPNKRAKDVGNSNGYSIIHGNLELSSQVQSGYLNKL 300

Query: 301 NDQQTAVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRV 360
            D++T++SSI KKDQE S VT++Q    SDGEGGSE ETGVNRKDEDEP AKRRN EVR 
Sbjct: 301 -DEETSISSIRKKDQELSRVTNDQFLGNSDGEGGSETETGVNRKDEDEPAAKRRNIEVRN 360

Query: 361 SEPATSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVR 420
           SEPA+SHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQK+VKGNPYPRSYYKCTTPGCNVR
Sbjct: 361 SEPASSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKIVKGNPYPRSYYKCTTPGCNVR 420

Query: 421 KHVERASTDQKAVITTYEGKHNHDVPAGKISSHSTVNSNVPQLKSQNIATD-----NNTD 480
           KHVERASTD KAVITTYEGKHNHDVP GK SSHS+V+SN+ QLKSQNI T+     NNTD
Sbjct: 421 KHVERASTDPKAVITTYEGKHNHDVPLGKTSSHSSVSSNISQLKSQNIVTEKKNSSNNTD 480

Query: 481 PGNSRQQPVGLLRLKKEQIT 495
            GNSRQQP GLLRLK+EQIT
Sbjct: 481 RGNSRQQPTGLLRLKEEQIT 492

BLAST of Lag0040147 vs. TAIR 10
Match: AT1G13960.1 (WRKY DNA-binding protein 4 )

HSP 1 Score: 442.2 bits (1136), Expect = 5.5e-124
Identity = 282/538 (52.42%), Postives = 330/538 (61.34%), Query Frame = 0

Query: 15  PPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVSSFFSDSDDCKSFSQ 74
           P  SKS   P RPT++LPPR   E  F+G    G GFSPGPMTLVS+ F DSD+ +SFSQ
Sbjct: 8   PSTSKSTGAPSRPTLSLPPRPFSEMFFNG----GVGFSPGPMTLVSNMFPDSDEFRSFSQ 67

Query: 75  LLAGAMASPVA--------------------------VPPRASELK-------------- 134
           LLAGAM+SP                            V PR  + +              
Sbjct: 68  LLAGAMSSPATAAAAAAAATASDYQRLGEGTNSSSGDVDPRFKQNRPTGLMISQSQSPSM 127

Query: 135 -------GPAGLLDSP---GLFSPGQGPFEMTHQQALAQVTMQAAQTYSHKQIQGESFSS 194
                   PA LLDSP   GLFSP QG + MTHQQALAQVT QA Q  ++ Q Q E    
Sbjct: 128 FTVPPGLSPAMLLDSPSFLGLFSPVQGSYGMTHQQALAQVTAQAVQANANMQPQTEY--- 187

Query: 195 LVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVASKESSDISQSDQRLQSSSCNVDRPA 254
              P  S  +  ++   +     P+P        A +E+SD++  + R Q    NVD+PA
Sbjct: 188 ---PPPSQVQSFSSGQAQIPTSAPLP--------AQRETSDVTIIEHRSQ-QPLNVDKPA 247

Query: 255 DDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKKVERSLEGQVIEIIYKGEHNHKRPQ 314
           DDGYNWRKYGQKQVKGSEFPRSYYKCT+P CPVKKKVERSL+GQV EIIYKG+HNH+ PQ
Sbjct: 248 DDGYNWRKYGQKQVKGSEFPRSYYKCTNPGCPVKKKVERSLDGQVTEIIYKGQHNHEPPQ 307

Query: 315 PNKRAK--DVGNSNGCS--SIHGNPEL-SSQVQSGYLNKLNDQQTAVSSIAKKDQESSHV 374
             KR    +  N NG S  +  G+ EL +SQ Q+   NK   +Q         +  S   
Sbjct: 308 NTKRGNKDNTANINGSSINNNRGSSELGASQFQTNSSNKTKREQ--------HEAVSQAT 367

Query: 375 THEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRVSE--PATSHRTLTESRIIVQ 434
           T E LS  SDGE     ET V  KDE+EPD KRR+TEVR+SE  PA SHRT+TE RIIVQ
Sbjct: 368 TTEHLSEASDGEEVGNGETDVREKDENEPDPKRRSTEVRISEPAPAASHRTVTEPRIIVQ 427

Query: 435 TTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRKHVERASTDQKAVITTYE 494
           TTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGC VRKHVERA+TD KAV+TTYE
Sbjct: 428 TTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCGVRKHVERAATDPKAVVTTYE 487

BLAST of Lag0040147 vs. TAIR 10
Match: AT2G03340.1 (WRKY DNA-binding protein 3 )

HSP 1 Score: 438.0 bits (1125), Expect = 1.0e-122
Identity = 292/542 (53.87%), Postives = 331/542 (61.07%), Query Frame = 0

Query: 15  PPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVSSFFSDSDDCKSFSQ 74
           P   KS     RPTI+LPPR   E  FSG    G GFSPGPMTLVS+ FSD D+ KSFSQ
Sbjct: 9   PSKLKSSTGVSRPTISLPPRPFGEMFFSG----GVGFSPGPMTLVSNLFSDPDEFKSFSQ 68

Query: 75  LLAGAMASPVA------------------------------VPPRASELK---------- 134
           LLAGAMASP A                              V PR  + +          
Sbjct: 69  LLAGAMASPAAAAVAAAAVVATAHHQTPVSSVGDGGGSGGDVDPRFKQSRPTGLMITQPP 128

Query: 135 ---------GPAGLLDSP---GLFSPGQGPFEMTHQQALAQVTMQAAQ---TYSHKQIQG 194
                     PA LLDSP   GLFSP QG F MTHQQALAQVT QA Q    +  +  Q 
Sbjct: 129 GMFTVPPGLSPATLLDSPSFFGLFSPLQGTFGMTHQQALAQVTAQAVQGNNVHMQQSQQS 188

Query: 195 ESFSSLVPPSASSSEL-LTTFPG-EKTKDQPVPPSLLNSAVASKESSDISQSDQRLQSSS 254
           E  SS         +  LT  P         +  S+  ++   +E+S+IS  + R Q   
Sbjct: 189 EYPSSTQQQQQQQQQASLTEIPSFSSAPRSQIRASVQETSQGQRETSEISVFEHRSQPQ- 248

Query: 255 CNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKKVERSLEGQVIEIIYKGE 314
            N D+PADDGYNWRKYGQKQVKGS+FPRSYYKCTHP CPVKKKVERSL+GQV EIIYKG+
Sbjct: 249 -NADKPADDGYNWRKYGQKQVKGSDFPRSYYKCTHPACPVKKKVERSLDGQVTEIIYKGQ 308

Query: 315 HNHKRPQPNKRAKDVGNSNGCSSIHGNPELSSQVQSGYLNKLNDQQTAVSSI--AKKDQE 374
           HNH+ PQ  KR    GN+NG          SS +        N  QT+ SS+  +K+DQE
Sbjct: 309 HNHELPQ--KR----GNNNGSCK-------SSDI-------ANQFQTSNSSLNKSKRDQE 368

Query: 375 SSHV-THEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRVSEP-ATSHRTLTESR 434
           +S V T EQ+S  SD E     ET V  + EDEPD KRRNTEVRVSEP A+SHRT+TE R
Sbjct: 369 TSQVTTTEQMSEASDSEEVGNAETSVGERHEDEPDPKRRNTEVRVSEPVASSHRTVTEPR 428

Query: 435 IIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRKHVERASTDQKAVI 494
           IIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTP C VRKHVERA+TD KAV+
Sbjct: 429 IIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPDCGVRKHVERAATDPKAVV 488

BLAST of Lag0040147 vs. TAIR 10
Match: AT1G13960.2 (WRKY DNA-binding protein 4 )

HSP 1 Score: 433.3 bits (1113), Expect = 2.6e-121
Identity = 273/514 (53.11%), Postives = 324/514 (63.04%), Query Frame = 0

Query: 15  PPPSKSKALPLRPTINLPPRTCMESLFSGGPGLGSGFSPGPMTLVSSFFSDSDDCKSFSQ 74
           P  SKS   P RPT++LPPR   E  F+G    G GFSPGPMTLVS+ F DSD+ +SFSQ
Sbjct: 8   PSTSKSTGAPSRPTLSLPPRPFSEMFFNG----GVGFSPGPMTLVSNMFPDSDEFRSFSQ 67

Query: 75  LLAGAMASPVA--------------------------VPPRASELKGPAGLLDSPGLFSP 134
           LLAGAM+SP                            V PR  + + P  +LD   + + 
Sbjct: 68  LLAGAMSSPATAAAAAAAATASDYQRLGEGTNSSSGDVDPRFKQNR-PTAVLDL--ICNI 127

Query: 135 GQGPFEMTHQQALAQVTMQAAQTYSHKQIQGESFSSLVPPSASSSELLTTFPGEKTKDQP 194
            QG + MTHQQALAQVT QA Q  ++ Q Q E       P  S  +  ++   +     P
Sbjct: 128 VQGSYGMTHQQALAQVTAQAVQANANMQPQTEY------PPPSQVQSFSSGQAQIPTSAP 187

Query: 195 VPPSLLNSAVASKESSDISQSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYY 254
           +P        A +E+SD++  + R Q    NVD+PADDGYNWRKYGQKQVKGSEFPRSYY
Sbjct: 188 LP--------AQRETSDVTIIEHRSQ-QPLNVDKPADDGYNWRKYGQKQVKGSEFPRSYY 247

Query: 255 KCTHPNCPVKKKVERSLEGQVIEIIYKGEHNHKRPQPNKRAK--DVGNSNGCS--SIHGN 314
           KCT+P CPVKKKVERSL+GQV EIIYKG+HNH+ PQ  KR    +  N NG S  +  G+
Sbjct: 248 KCTNPGCPVKKKVERSLDGQVTEIIYKGQHNHEPPQNTKRGNKDNTANINGSSINNNRGS 307

Query: 315 PEL-SSQVQSGYLNKLNDQQTAVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRK 374
            EL +SQ Q+   NK   +Q         +  S   T E LS  SDGE     ET V  K
Sbjct: 308 SELGASQFQTNSSNKTKREQ--------HEAVSQATTTEHLSEASDGEEVGNGETDVREK 367

Query: 375 DEDEPDAKRRNTEVRVSE--PATSHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKG 434
           DE+EPD KRR+TEVR+SE  PA SHRT+TE RIIVQTTSEVDLLDDGYRWRKYGQKVVKG
Sbjct: 368 DENEPDPKRRSTEVRISEPAPAASHRTVTEPRIIVQTTSEVDLLDDGYRWRKYGQKVVKG 427

Query: 435 NPYPRSYYKCTTPGCNVRKHVERASTDQKAVITTYEGKHNHDVPAGKISSHSTVNSNV-P 494
           NPYPRSYYKCTTPGC VRKHVERA+TD KAV+TTYEGKHNHD+PA K SSH+   + + P
Sbjct: 428 NPYPRSYYKCTTPGCGVRKHVERAATDPKAVVTTYEGKHNHDLPAAKSSSHAAAAAQLRP 487

BLAST of Lag0040147 vs. TAIR 10
Match: AT3G01080.1 (WRKY DNA-binding protein 58 )

HSP 1 Score: 289.7 bits (740), Expect = 4.6e-78
Identity = 209/502 (41.63%), Postives = 260/502 (51.79%), Query Frame = 0

Query: 24  PLRPTINLPPR-TCMES---LFSGGPGLGSGFSPGPMTLVSSFFSDSDDCKSFSQLLAGA 83
           P RPTI +P R   +E+    F GG GL    SPGP++ VSS F D+             
Sbjct: 18  PTRPTITVPHRPPAIETAAYFFGGGDGL--SLSPGPLSFVSSLFVDN------------- 77

Query: 84  MASPVAVPPRASELKGPAGLLDSPGLFSPGQGPFEMTHQQALAQVTMQAAQTYSHKQIQG 143
              P  + P          LL SP  F P         Q +     +Q  Q    +  + 
Sbjct: 78  --FPDVLTPDNQRTTSFTHLLTSPMFFPP---------QSSAHTGFIQPRQQSQPQPQRP 137

Query: 144 ESFSSLVPPSASSSELLTTFPGEKTKDQPVPPSLLNSAVASKESSDISQSDQRLQS---- 203
           ++F   +PPS S                         AV  ++S D+SQ DQR ++    
Sbjct: 138 DTFPHHMPPSTS------------------------VAVHGRQSLDVSQVDQRARNHYNN 197

Query: 204 ----------SSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKKVERSL 263
                     +  NVD+PADDGYNWRKYGQK +KG E+PRSYYKCTH NCPVKKKVERS 
Sbjct: 198 PGNNNNNRSYNVVNVDKPADDGYNWRKYGQKPIKGCEYPRSYYKCTHVNCPVKKKVERSS 257

Query: 264 EGQVIEIIYKGEHNHKRPQPNKRAKDVGNSNGCSSIHGNPE-LSSQVQSGYLNKLNDQQT 323
           +GQ+ +IIYKG+H+H+RPQ N+R    G     + + G  + + S   SGY         
Sbjct: 258 DGQITQIIYKGQHDHERPQ-NRRG---GGGRDSTEVGGAGQMMESSDDSGY--------- 317

Query: 324 AVSSIAKKDQESSHVTHEQLSSTSDGEGGSEIETGVNRKDEDEPDAKRRNTEVRVSEPAT 383
                 +KD +      E                     DED P +K R    R+   +T
Sbjct: 318 ------RKDHDDDDDDDED--------------------DEDLPASKIR----RIDGVST 377

Query: 384 SHRTLTESRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRKHVER 443
           +HRT+TE +IIVQT SEVDLLDDGYRWRKYGQKVVKGNP+PRSYYKCTTP C VRKHVER
Sbjct: 378 THRTVTEPKIIVQTKSEVDLLDDGYRWRKYGQKVVKGNPHPRSYYKCTTPNCTVRKHVER 422

Query: 444 ASTDQKAVITTYEGKHNHDVPAGK------------ISSHSTVNSNVPQLKSQNIATDNN 495
           ASTD KAVITTYEGKHNHDVPA +             S H  + S       Q+++  NN
Sbjct: 438 ASTDAKAVITTYEGKHNHDVPAARNGTAAATAAAVGPSDHHRMRSMSGNNMQQHMSFGNN 422

BLAST of Lag0040147 vs. TAIR 10
Match: AT5G56270.1 (WRKY DNA-binding protein 2 )

HSP 1 Score: 260.0 bits (663), Expect = 3.9e-69
Identity = 156/336 (46.43%), Postives = 194/336 (57.74%), Query Frame = 0

Query: 188 QSDQRLQSSSCNVDRPADDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCPVKKKVERSLEG 247
           + +++ +  S     PA+DGYNWRKYGQK VKGSE+PRSYYKCT+PNC VKKKVERS EG
Sbjct: 256 EEEEQRRGDSMAGGAPAEDGYNWRKYGQKLVKGSEYPRSYYKCTNPNCQVKKKVERSREG 315

Query: 248 QVIEIIYKGEHNHKRPQPNKRA-----------------------------KDVGNSN-- 307
            + EIIYKG HNH +P PN+R+                             +  G SN  
Sbjct: 316 HITEIIYKGAHNHLKPPPNRRSGMQVDGTEQVEQQQQQRDSAATWVSCNNTQQQGGSNEN 375

Query: 308 ----GCSSI-HGNPELSSQVQSGYLNKLNDQQTAV---SSIAKKDQESSHVTHEQLSSTS 367
               G +   +GN   S Q Q+G   +  D    V   S+ +  + E    TH  +S   
Sbjct: 376 NVEEGSTRFEYGNQSGSIQAQTGGQYESGDPVVVVDASSTFSNDEDEDDRGTHGSVSLGY 435

Query: 368 DGEGGSEIETGVNRKDEDEPDAKRRNTEVRVSEPATSHRTLTESRIIVQTTSEVDLLDDG 427
           DG GG     G      DE ++KRR  E   +E + S R + E R++VQTTS+VD+LDDG
Sbjct: 436 DGGGGGGGGEG------DESESKRRKLEAFAAEMSGSTRAIREPRVVVQTTSDVDILDDG 495

Query: 428 YRWRKYGQKVVKGNPYPRSYYKCTTPGCNVRKHVERASTDQKAVITTYEGKHNHDVPAGK 485
           YRWRKYGQKVVKGNP PRSYYKCT PGC VRKHVERAS D K+VITTYEGKHNHDVPA +
Sbjct: 496 YRWRKYGQKVVKGNPNPRSYYKCTAPGCTVRKHVERASHDLKSVITTYEGKHNHDVPAAR 555

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008446405.25.7e-22482.80PREDICTED: probable WRKY transcription factor 3 isoform X1 [Cucumis melo] >TYK17... [more]
XP_022151251.15.4e-22283.86probable WRKY transcription factor 3 [Momordica charantia][more]
KAG7032241.13.6e-21882.19putative WRKY transcription factor 3 [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_023515298.13.0e-21781.98probable WRKY transcription factor 4 [Cucurbita pepo subsp. pepo][more]
XP_022957438.13.4e-21682.19probable WRKY transcription factor 4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9XI907.7e-12352.42Probable WRKY transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=WRKY4 PE... [more]
Q9ZQ701.5e-12153.87Probable WRKY transcription factor 3 OS=Arabidopsis thaliana OX=3702 GN=WRKY3 PE... [more]
Q93WU76.4e-7741.63Probable WRKY transcription factor 58 OS=Arabidopsis thaliana OX=3702 GN=WRKY58 ... [more]
Q9FG775.5e-6846.43Probable WRKY transcription factor 2 OS=Arabidopsis thaliana OX=3702 GN=WRKY2 PE... [more]
Q8S8P51.1e-6544.90Probable WRKY transcription factor 33 OS=Arabidopsis thaliana OX=3702 GN=WRKY33 ... [more]
Match NameE-valueIdentityDescription
A0A5D3D0G92.8e-22482.80Putative WRKY transcription factor 3 isoform X1 OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3BEZ92.8e-22482.80probable WRKY transcription factor 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1DE242.6e-22283.86probable WRKY transcription factor 3 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A6J1H0781.6e-21682.19probable WRKY transcription factor 4 OS=Cucurbita moschata OX=3662 GN=LOC1114588... [more]
A0A0A0KW352.6e-21481.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G606470 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G13960.15.5e-12452.42WRKY DNA-binding protein 4 [more]
AT2G03340.11.0e-12253.87WRKY DNA-binding protein 3 [more]
AT1G13960.22.6e-12153.11WRKY DNA-binding protein 4 [more]
AT3G01080.14.6e-7841.63WRKY DNA-binding protein 58 [more]
AT5G56270.13.9e-6946.43WRKY DNA-binding protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 204..262
e-value: 6.7E-38
score: 141.9
coord: 385..444
e-value: 1.6E-38
score: 144.0
IPR003657WRKY domainPFAMPF03106WRKYcoord: 205..261
e-value: 2.2E-26
score: 91.9
coord: 386..443
e-value: 5.4E-25
score: 87.4
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 205..263
score: 23.525288
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 380..445
score: 38.411476
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 188..264
e-value: 2.6E-28
score: 99.6
coord: 370..446
e-value: 4.0E-36
score: 124.6
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 203..263
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 377..445
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 320..334
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 254..292
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 306..370
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..202
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 140..160
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 269..292
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 465..494
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 254..268
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 140..215
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 465..480
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 336..360
NoneNo IPR availablePANTHERPTHR31221:SF192WRKY TRANSCRIPTION FACTOR 58-RELATEDcoord: 21..493
IPR044810WRKY transcription factor, plantPANTHERPTHR31221WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATEDcoord: 21..493

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040147.1Lag0040147.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding