Lag0040133 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040133
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionMitochondrial uncoupling protein
Locationchr13: 2408799 .. 2410224 (+)
RNA-Seq ExpressionLag0040133
SyntenyLag0040133
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATCAAGCGAAGAACATGGACGCCACAATCCATACACCAAATTGTTGCTCACCTCCCTCTCCGCCATGGTGGCCGAGTCCACCACCTTCCCCATAGACCTCACGAAGACCAGGCTTCAGCTCCACGGCGAGTCCTCCTCTTCCTCTCGCTCCACAAATGCCTTCAGACTCGCTTCAGCCATCGTCAAGGACCAAGGTCCTTTTGGCCTTTACAAGGGCTTGTCTCCGGCGATTCTCAGGCATCTCTTCTATACTCCCATACGAATCGTTGGATACGAGCACCTACGATCCCTCTTCCTTGCCTCCGATGGCGGCTCTGTTTCCCTCCATGCCAAGGCCCTTGTCGGTGGAATCTCTGGCTCCATTGCTCAGGTTCGAATTTTCCACATTATGCATTTAGGAGTCTGTGATTAGCGATATGGTACACTGTAGAGACTTGCATTTGTCAATGAGCTGCAATGTGGAGAACTGTAGCTAATCGAGTTATGCACAATTCATGAAATGTATAATCGCTGGTACCTGTGCCGATCTTTCAATTTGTAATATGTTGAAATTGCCATTTTAGAATCAAGGATGATTAGGAAGATCAGAGGGACTTTCTGAACTTTCTGTAAGCCTTGAGCTATGTTAAACTAGTCCACTAAATCCTAATACTCCCATCATTTTGTGAGTTTGGAAATAGGTATAAGGCTCAATGAATTATCATCAATATTAATTAGGAAGGGAATGACACAAAATGAATTCAAACACGACTTTTCTACTCTGGTATTTAAAAAATAACATTGAACTCAAAAACTTGCATCTAAATTACTGTAAATTCTTAACATTCTACTTCTTTGCTAAATATCAAATACTTTTGTGTCTACCATTAGTACCACAGGTAGTGGCAAGTCCTGCTGATCTTGTTAAGGTGAGGATGCAAGCTGATGGCCGTCTAATGAGCCAAGGTCTGCAACCCAGATACTCAGGACCTCTCGATGCCCTAACCAAGATAGTGCGTGCAGAAGGGATAGTTGGGCTTTGGAAAGGGGTTGTCCCAAATGTTCAAAGAGCTTTTTTGGTTAACATGGGAGAATTAGCCTGCTATGACCATGCAAAACGCTTTGTTATTCAAAATCAAATAGCTGGGGATAACATTTTTGGCCACAGCTTTGCTTCTGTAATTTCGGGTCTGTGTGCAACTGCACTGAGTTGTCCTGCTGATGTTGTGAAGACAAGGATGATGAATCAAGCTGCTAGCAAAGAAGGTACGATCAAGTACAACAGCTCGTATGATTGTCTTATGAAGACTGTTAAAGTTGAAGGATTGAGAGCTCTGTGGAAGGGGTTTTTTCCCACTTGGGCGAGGCTTGGTCCTTGGCAATTTGTCTTCTGGGTGTCTTATGAGAAGTTTCGCAAACTTGCTGGGTTAGCTTCCTTCTAG

mRNA sequence

ATGAAATCAAGCGAAGAACATGGACGCCACAATCCATACACCAAATTGTTGCTCACCTCCCTCTCCGCCATGGTGGCCGAGTCCACCACCTTCCCCATAGACCTCACGAAGACCAGGCTTCAGCTCCACGGCGAGTCCTCCTCTTCCTCTCGCTCCACAAATGCCTTCAGACTCGCTTCAGCCATCGTCAAGGACCAAGGTCCTTTTGGCCTTTACAAGGGCTTGTCTCCGGCGATTCTCAGGCATCTCTTCTATACTCCCATACGAATCGTTGGATACGAGCACCTACGATCCCTCTTCCTTGCCTCCGATGGCGGCTCTGTTTCCCTCCATGCCAAGGCCCTTGTCGGTGGAATCTCTGGCTCCATTGCTCAGGTAGTGGCAAGTCCTGCTGATCTTGTTAAGGTGAGGATGCAAGCTGATGGCCGTCTAATGAGCCAAGGTCTGCAACCCAGATACTCAGGACCTCTCGATGCCCTAACCAAGATAGTGCGTGCAGAAGGGATAGTTGGGCTTTGGAAAGGGGTTGTCCCAAATGTTCAAAGAGCTTTTTTGGTTAACATGGGAGAATTAGCCTGCTATGACCATGCAAAACGCTTTGTTATTCAAAATCAAATAGCTGGGGATAACATTTTTGGCCACAGCTTTGCTTCTGTAATTTCGGGTCTGTGTGCAACTGCACTGAGTTGTCCTGCTGATGTTGTGAAGACAAGGATGATGAATCAAGCTGCTAGCAAAGAAGGTACGATCAAGTACAACAGCTCGTATGATTGTCTTATGAAGACTGTTAAAGTTGAAGGATTGAGAGCTCTGTGGAAGGGGTTTTTTCCCACTTGGGCGAGGCTTGGTCCTTGGCAATTTGTCTTCTGGGTGTCTTATGAGAAGTTTCGCAAACTTGCTGGGTTAGCTTCCTTCTAG

Coding sequence (CDS)

ATGAAATCAAGCGAAGAACATGGACGCCACAATCCATACACCAAATTGTTGCTCACCTCCCTCTCCGCCATGGTGGCCGAGTCCACCACCTTCCCCATAGACCTCACGAAGACCAGGCTTCAGCTCCACGGCGAGTCCTCCTCTTCCTCTCGCTCCACAAATGCCTTCAGACTCGCTTCAGCCATCGTCAAGGACCAAGGTCCTTTTGGCCTTTACAAGGGCTTGTCTCCGGCGATTCTCAGGCATCTCTTCTATACTCCCATACGAATCGTTGGATACGAGCACCTACGATCCCTCTTCCTTGCCTCCGATGGCGGCTCTGTTTCCCTCCATGCCAAGGCCCTTGTCGGTGGAATCTCTGGCTCCATTGCTCAGGTAGTGGCAAGTCCTGCTGATCTTGTTAAGGTGAGGATGCAAGCTGATGGCCGTCTAATGAGCCAAGGTCTGCAACCCAGATACTCAGGACCTCTCGATGCCCTAACCAAGATAGTGCGTGCAGAAGGGATAGTTGGGCTTTGGAAAGGGGTTGTCCCAAATGTTCAAAGAGCTTTTTTGGTTAACATGGGAGAATTAGCCTGCTATGACCATGCAAAACGCTTTGTTATTCAAAATCAAATAGCTGGGGATAACATTTTTGGCCACAGCTTTGCTTCTGTAATTTCGGGTCTGTGTGCAACTGCACTGAGTTGTCCTGCTGATGTTGTGAAGACAAGGATGATGAATCAAGCTGCTAGCAAAGAAGGTACGATCAAGTACAACAGCTCGTATGATTGTCTTATGAAGACTGTTAAAGTTGAAGGATTGAGAGCTCTGTGGAAGGGGTTTTTTCCCACTTGGGCGAGGCTTGGTCCTTGGCAATTTGTCTTCTGGGTGTCTTATGAGAAGTTTCGCAAACTTGCTGGGTTAGCTTCCTTCTAG

Protein sequence

MKSSEEHGRHNPYTKLLLTSLSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGISGSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNVQRAFLVNMGELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMMNQAASKEGTIKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLASF
Homology
BLAST of Lag0040133 vs. NCBI nr
Match: XP_038892574.1 (mitochondrial uncoupling protein 3 [Benincasa hispida])

HSP 1 Score: 573.9 bits (1478), Expect = 8.0e-160
Identity = 284/305 (93.11%), Postives = 295/305 (96.72%), Query Frame = 0

Query: 1   MKSSEEHGRHNPYTKLLLTSLSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLAS 60
           MK+S+EHGR+NPY KL LT+LSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLAS
Sbjct: 1   MKTSDEHGRYNPYAKLFLTALSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLAS 60

Query: 61  AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGIS 120
           AIVKDQGPF LYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSV LH KALVGG+S
Sbjct: 61  AIVKDQGPFALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVPLHVKALVGGLS 120

Query: 121 GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNV 180
           GSIAQVVASPADLVKVRMQADGRL+SQGLQPRYSGPLDALTKIV  EGI+GLWKGVVPNV
Sbjct: 121 GSIAQVVASPADLVKVRMQADGRLVSQGLQPRYSGPLDALTKIVHGEGIIGLWKGVVPNV 180

Query: 181 QRAFLVNMGELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMM 240
           QRAFLVNMGELACYDHAKRFV+QNQIAGDNIFGH+FASVISGLCATALSCPADVVKTRMM
Sbjct: 181 QRAFLVNMGELACYDHAKRFVLQNQIAGDNIFGHTFASVISGLCATALSCPADVVKTRMM 240

Query: 241 NQAASKEGTIKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA 300
           NQA SKEG  KYNSSYDCL+KTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA
Sbjct: 241 NQAVSKEGIAKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA 300

Query: 301 GLASF 306
           GL+SF
Sbjct: 301 GLSSF 305

BLAST of Lag0040133 vs. NCBI nr
Match: XP_022957541.1 (mitochondrial uncoupling protein 3 [Cucurbita moschata])

HSP 1 Score: 568.9 bits (1465), Expect = 2.6e-158
Identity = 283/305 (92.79%), Postives = 296/305 (97.05%), Query Frame = 0

Query: 1   MKSSEEHGRHNPYTKLLLTSLSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLAS 60
           MK+SEEHGRHNP TK+LLT+LSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFR+AS
Sbjct: 1   MKTSEEHGRHNPNTKMLLTALSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRVAS 60

Query: 61  AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGIS 120
           AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSL AKALVGGIS
Sbjct: 61  AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLPAKALVGGIS 120

Query: 121 GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNV 180
           GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDA TKIVR EGI+GLWKGVVPNV
Sbjct: 121 GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDAFTKIVRGEGIMGLWKGVVPNV 180

Query: 181 QRAFLVNMGELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMM 240
           QRAFLVNMGELACYDHAKRFVI+NQIAGDNIFGH+ ASV+SGL ATALSCPADVVKTRMM
Sbjct: 181 QRAFLVNMGELACYDHAKRFVIRNQIAGDNIFGHTLASVMSGLSATALSCPADVVKTRMM 240

Query: 241 NQAASKEGTIKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA 300
           NQAASKEGT  YN +YDCL+KTVK+EGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA
Sbjct: 241 NQAASKEGTTMYNGAYDCLVKTVKIEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA 300

Query: 301 GLASF 306
           G++SF
Sbjct: 301 GISSF 305

BLAST of Lag0040133 vs. NCBI nr
Match: KAG7032229.1 (Mitochondrial uncoupling protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 567.8 bits (1462), Expect = 5.8e-158
Identity = 282/305 (92.46%), Postives = 296/305 (97.05%), Query Frame = 0

Query: 1   MKSSEEHGRHNPYTKLLLTSLSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLAS 60
           MK+SEEHGRHNP TK+LLT+L+AMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFR+AS
Sbjct: 1   MKTSEEHGRHNPNTKMLLTALAAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRVAS 60

Query: 61  AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGIS 120
           AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSL AKALVGGIS
Sbjct: 61  AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLPAKALVGGIS 120

Query: 121 GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNV 180
           GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDA TKIVR EGI+GLWKGVVPNV
Sbjct: 121 GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDAFTKIVRGEGIMGLWKGVVPNV 180

Query: 181 QRAFLVNMGELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMM 240
           QRAFLVNMGELACYDHAKRFVI+NQIAGDNIFGH+ ASV+SGL ATALSCPADVVKTRMM
Sbjct: 181 QRAFLVNMGELACYDHAKRFVIRNQIAGDNIFGHTLASVMSGLSATALSCPADVVKTRMM 240

Query: 241 NQAASKEGTIKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA 300
           NQAASKEGT  YN +YDCL+KTVK+EGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA
Sbjct: 241 NQAASKEGTTMYNGTYDCLVKTVKIEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA 300

Query: 301 GLASF 306
           G++SF
Sbjct: 301 GISSF 305

BLAST of Lag0040133 vs. NCBI nr
Match: XP_023513662.1 (mitochondrial uncoupling protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 566.6 bits (1459), Expect = 1.3e-157
Identity = 281/305 (92.13%), Postives = 296/305 (97.05%), Query Frame = 0

Query: 1   MKSSEEHGRHNPYTKLLLTSLSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLAS 60
           MK+SEEHGRHNP TK+LLT+L+AMVAESTTFPIDLTKTRLQLHGES+SSSRSTNAFR+AS
Sbjct: 1   MKTSEEHGRHNPNTKMLLTALAAMVAESTTFPIDLTKTRLQLHGESASSSRSTNAFRVAS 60

Query: 61  AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGIS 120
           AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSL AKALVGGIS
Sbjct: 61  AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLPAKALVGGIS 120

Query: 121 GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNV 180
           GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDA TKIVR EGI+GLWKGVVPNV
Sbjct: 121 GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDAFTKIVRGEGIMGLWKGVVPNV 180

Query: 181 QRAFLVNMGELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMM 240
           QRAFLVNMGELACYDHAKRFVI+NQIAGDNIFGH+ ASV+SGL ATALSCPADVVKTRMM
Sbjct: 181 QRAFLVNMGELACYDHAKRFVIRNQIAGDNIFGHTLASVMSGLSATALSCPADVVKTRMM 240

Query: 241 NQAASKEGTIKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA 300
           NQAASKEGT  YN +YDCL+KTVK+EGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA
Sbjct: 241 NQAASKEGTTMYNGAYDCLVKTVKIEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA 300

Query: 301 GLASF 306
           G++SF
Sbjct: 301 GISSF 305

BLAST of Lag0040133 vs. NCBI nr
Match: XP_004135167.1 (mitochondrial uncoupling protein 3 [Cucumis sativus] >KAE8648752.1 hypothetical protein Csa_009240 [Cucumis sativus])

HSP 1 Score: 561.2 bits (1445), Expect = 5.4e-156
Identity = 279/296 (94.26%), Postives = 287/296 (96.96%), Query Frame = 0

Query: 10  HNPYTKLLLTSLSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF 69
           HNPYTKL+LT LSAMVAES TFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF
Sbjct: 5   HNPYTKLVLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF 64

Query: 70  GLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGISGSIAQVVAS 129
            LYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVS H+KALVGGISGSIAQVVAS
Sbjct: 65  ALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSFHSKALVGGISGSIAQVVAS 124

Query: 130 PADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNVQRAFLVNMG 189
           PADLVKVRMQADGRL+SQGLQPRYSGP DALTKIVR EG+VGLWKGVVPNVQRAFLVNMG
Sbjct: 125 PADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMG 184

Query: 190 ELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMMNQAASKEGT 249
           ELACYDHAKRFVIQNQ+AGDNIFGH+ ASVISGLCATALSCPADVVKTRMMNQAASKEG 
Sbjct: 185 ELACYDHAKRFVIQNQLAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGI 244

Query: 250 IKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLASF 306
            KYNSSYDCL+KTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGL+SF
Sbjct: 245 TKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300

BLAST of Lag0040133 vs. ExPASy Swiss-Prot
Match: Q9XI74 (Mitochondrial uncoupling protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUMP3 PE=2 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 1.1e-116
Identity = 210/296 (70.95%), Postives = 247/296 (83.45%), Query Frame = 0

Query: 14  TKLLLTSLSAMVAESTTFPIDLTKTRLQLHGE-SSSSSRSTNAFRLASAIVKDQGPFGLY 73
           T++LL SLSAMVAES TFPIDLTKTR+QLHG  S+S +    AF + S I + +G  GLY
Sbjct: 14  TRILLASLSAMVAESVTFPIDLTKTRMQLHGSGSASGAHRIGAFGVVSEIARKEGVIGLY 73

Query: 74  KGLSPAILRHLFYTPIRIVGYEHLRSLFLASD---GGSVSLHAKALVGGISGSIAQVVAS 133
           KGLSPAI+RHLFYTPIRI+GYE+L+ L + S+     S+ L  KALVGG SG IAQVVAS
Sbjct: 74  KGLSPAIIRHLFYTPIRIIGYENLKGLIVRSETNNSESLPLATKALVGGFSGVIAQVVAS 133

Query: 134 PADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNVQRAFLVNMG 193
           PADLVKVRMQADGRL+SQGL+PRYSGP++A TKI+++EG+ GLWKGV+PN+QRAFLVNMG
Sbjct: 134 PADLVKVRMQADGRLVSQGLKPRYSGPIEAFTKILQSEGVKGLWKGVLPNIQRAFLVNMG 193

Query: 194 ELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMMNQAASKEGT 253
           ELACYDHAK FVI  +IA DNIF H+ AS++SGL +T+LSCPADVVKTRMMNQ  +    
Sbjct: 194 ELACYDHAKHFVIDKKIAEDNIFAHTLASIMSGLASTSLSCPADVVKTRMMNQGEN---- 253

Query: 254 IKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLASF 306
             Y +SYDCL+KTVK EG+RALWKGFFPTWARLGPWQFVFWVSYEKFR LAG++SF
Sbjct: 254 AVYRNSYDCLVKTVKFEGIRALWKGFFPTWARLGPWQFVFWVSYEKFRLLAGISSF 305

BLAST of Lag0040133 vs. ExPASy Swiss-Prot
Match: O95847 (Mitochondrial uncoupling protein 4 OS=Homo sapiens OX=9606 GN=SLC25A27 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 7.8e-65
Identity = 139/323 (43.03%), Postives = 197/323 (60.99%), Query Frame = 0

Query: 1   MKSSEEHGRHNPYT-------KLLLTSLSAMVAESTTFPIDLTKTRLQLHGESS------ 60
           M   EE  R  P T       K LL+  +A VAE  TFP+DLTKTRLQ+ GE++      
Sbjct: 1   MSVPEEEERLLPLTQRWPRASKFLLSGCAATVAELATFPLDLTKTRLQMQGEAALARLGD 60

Query: 61  ---SSSRSTNAFRLASAIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRS-LFLAS 120
               S+      R A  I++++G   L++G++PAI RH+ Y+  R+V YEHLR  +F  S
Sbjct: 61  GARESAPYRGMVRTALGIIEEEGFLKLWQGVTPAIYRHVVYSGGRMVTYEHLREVVFGKS 120

Query: 121 DGGSVSLHAKALVGGISGSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDALTKI 180
           +     L    + G ++G I Q +A+P DLVKV+MQ +G+   +G   R+ G   A  KI
Sbjct: 121 EDEHYPLWKSVIGGMMAGVIGQFLANPTDLVKVQMQMEGKRKLEGKPLRFRGVHHAFAKI 180

Query: 181 VRAEGIVGLWKGVVPNVQRAFLVNMGELACYDHAKRFVIQNQIAGDNIFGHSFASVISGL 240
           +   GI GLW G VPN+QRA LVNMG+L  YD  K +++ N    DNI  H  +S+ SGL
Sbjct: 181 LAEGGIRGLWAGWVPNIQRAALVNMGDLTTYDTVKHYLVLNTPLEDNIMTHGLSSLCSGL 240

Query: 241 CATALSCPADVVKTRMMNQAASKEGT-IKYNSSYDCLMKTVKVEGLRALWKGFFPTWARL 300
            A+ L  PADV+K+R+MNQ   K+G  + Y SS DCL++ V+ EG  +L+KGF P+W R+
Sbjct: 241 VASILGTPADVIKSRIMNQPRDKQGRGLLYKSSTDCLIQAVQGEGFMSLYKGFLPSWLRM 300

Query: 301 GPWQFVFWVSYEKFRKLAGLASF 306
            PW  VFW++YEK R+++G++ F
Sbjct: 301 TPWSMVFWLTYEKIREMSGVSPF 323

BLAST of Lag0040133 vs. ExPASy Swiss-Prot
Match: B0G143 (Mitochondrial substrate carrier family protein ucpB OS=Dictyostelium discoideum OX=44689 GN=ucpB PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 4.4e-52
Identity = 114/290 (39.31%), Postives = 171/290 (58.97%), Query Frame = 0

Query: 15  KLLLTSLSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPFGLYKG 74
           K L   LS M A   + P+D+ KTR Q+HGE    S+S         I+K++G   +YKG
Sbjct: 10  KFLFGGLSCMGAAVVSNPVDVLKTRFQIHGE-GIDSKSLGLVNGTIKIIKNEGISAMYKG 69

Query: 75  LSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGISGSIAQVVASPADLV 134
           L+P++LR   Y+ +R+ GY+ +++ F+ S+ G  +L +K   G +SG++   + SP DL+
Sbjct: 70  LTPSLLREATYSTLRMGGYDVIKNYFIDSN-GKTNLLSKVTSGALSGALGACITSPTDLI 129

Query: 135 KVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNVQRAFLVNMGELACY 194
           KVRMQA     S+G+  +Y     A  +I+  EGI GLWKGV P  QRA L+   ++  Y
Sbjct: 130 KVRMQAS----SKGV--KYDSISSAFKEIIAKEGIKGLWKGVGPTTQRAALLTASQIPSY 189

Query: 195 DHAKRFVIQNQIAG-DNIFGHSFASVISGLCATALSCPADVVKTRMMNQAASKEGT-IKY 254
           DH K  ++ + I   D +  H  +S+ +GL A+  + P D+VKTR+MNQ     G  + Y
Sbjct: 190 DHIKHMILDHGIIQVDGLQVHIVSSIFAGLIASITTSPVDLVKTRIMNQPFDSNGVGLIY 249

Query: 255 NSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGL 303
            SSYDC  KT + EG+  L+KGF P W R+GP   V ++ YE  RK++G+
Sbjct: 250 KSSYDCFKKTFQSEGISGLYKGFLPNWFRIGPHTIVTFILYEYLRKVSGI 291

BLAST of Lag0040133 vs. ExPASy Swiss-Prot
Match: Q6GQ22 (Kidney mitochondrial carrier protein 1 OS=Xenopus laevis OX=8355 GN=slc25a30 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.1e-49
Identity = 112/284 (39.44%), Postives = 168/284 (59.15%), Query Frame = 0

Query: 21  LSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFR-LASAIV---KDQGPFGLYKGLS 80
           L+++ AE  TFPIDLTKTRLQ+ G+ + +      +R +  AIV   +++G   LY G++
Sbjct: 14  LASITAECGTFPIDLTKTRLQVQGQPNDAKYKEIRYRGMMHAIVRIWREEGVKALYSGIA 73

Query: 81  PAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGISGSIAQVVASPADLVKV 140
           PA+LR   Y  I+I  Y+ L+ LF+       +L   A  G +SG ++  +A+P D++K+
Sbjct: 74  PAMLRQASYGTIKIGTYQSLKRLFVDCPEDE-TLVLNAFCGVLSGVVSSCIANPTDVLKI 133

Query: 141 RMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNVQRAFLVNMGELACYDH 200
           RMQA G +M  G+   +         I + EG  GLWKGV    QRA +V   EL  YD 
Sbjct: 134 RMQAQGNVMQGGMIVNF-------INIYQQEGTRGLWKGVSLTAQRAAIVVGVELPVYDI 193

Query: 201 AKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMMNQAASKEGT-IKYNSS 260
            K+ +I + + GD ++ H  +S   GL     S P DVV+TRMMNQ + ++ +   Y  +
Sbjct: 194 TKKHLILSGLMGDTVYTHFLSSFTCGLAGALASNPVDVVRTRMMNQRSIRDASNSSYKGT 253

Query: 261 YDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKL 300
            DCL++T K EG  AL+KGF+P W RLGPW  +F+++YE+ +KL
Sbjct: 254 LDCLLQTWKNEGFFALYKGFWPNWLRLGPWNIIFFITYEQLKKL 289

BLAST of Lag0040133 vs. ExPASy Swiss-Prot
Match: Q5XGI1 (Kidney mitochondrial carrier protein 1 OS=Xenopus tropicalis OX=8364 GN=slc25a30 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.1e-49
Identity = 113/284 (39.79%), Postives = 166/284 (58.45%), Query Frame = 0

Query: 21  LSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFR-LASAIV---KDQGPFGLYKGLS 80
           L+++ AE  TFPIDLTKTRLQ+ G+++ +      +R +  AIV   K++G   LY G++
Sbjct: 14  LASITAECGTFPIDLTKTRLQVQGQANDAKYKEIRYRGMLHAIVRIWKEEGVKALYSGIA 73

Query: 81  PAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGISGSIAQVVASPADLVKV 140
           PA+LR   Y  I+I  Y+ L+ LF+       +L      G +SG ++  +A+P D++K+
Sbjct: 74  PAMLRQASYGTIKIGTYQSLKRLFVDCPEDE-TLVINVFCGVLSGVVSSCIANPTDVLKI 133

Query: 141 RMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNVQRAFLVNMGELACYDH 200
           RMQA G L+  G+       +     I + EG  GLWKGV    QRA +V   EL  YD 
Sbjct: 134 RMQAQGSLIQGGM-------IGNFINIYQQEGTRGLWKGVSLTAQRAAIVVGVELPVYDI 193

Query: 201 AKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMMNQAASKE-GTIKYNSS 260
            K+ +I + + GD ++ H  AS   GL     S P DVV+TRMMNQ + +      Y  +
Sbjct: 194 TKKHLILSGLMGDTVYTHFLASFTCGLAGALASNPVDVVRTRMMNQRSIRNVSNSSYKGT 253

Query: 261 YDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKL 300
            DCL++T K EG  AL+KGF+P W RLGPW  +F+++YE+ +KL
Sbjct: 254 LDCLLQTWKNEGFFALYKGFWPNWLRLGPWNIIFFITYEQLKKL 289

BLAST of Lag0040133 vs. ExPASy TrEMBL
Match: A0A6J1GZH9 (mitochondrial uncoupling protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111458911 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.3e-158
Identity = 283/305 (92.79%), Postives = 296/305 (97.05%), Query Frame = 0

Query: 1   MKSSEEHGRHNPYTKLLLTSLSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLAS 60
           MK+SEEHGRHNP TK+LLT+LSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFR+AS
Sbjct: 1   MKTSEEHGRHNPNTKMLLTALSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRVAS 60

Query: 61  AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGIS 120
           AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSL AKALVGGIS
Sbjct: 61  AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLPAKALVGGIS 120

Query: 121 GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNV 180
           GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDA TKIVR EGI+GLWKGVVPNV
Sbjct: 121 GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDAFTKIVRGEGIMGLWKGVVPNV 180

Query: 181 QRAFLVNMGELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMM 240
           QRAFLVNMGELACYDHAKRFVI+NQIAGDNIFGH+ ASV+SGL ATALSCPADVVKTRMM
Sbjct: 181 QRAFLVNMGELACYDHAKRFVIRNQIAGDNIFGHTLASVMSGLSATALSCPADVVKTRMM 240

Query: 241 NQAASKEGTIKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA 300
           NQAASKEGT  YN +YDCL+KTVK+EGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA
Sbjct: 241 NQAASKEGTTMYNGAYDCLVKTVKIEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA 300

Query: 301 GLASF 306
           G++SF
Sbjct: 301 GISSF 305

BLAST of Lag0040133 vs. ExPASy TrEMBL
Match: A0A5A7SUW0 (Mitochondrial uncoupling protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1932G00220 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.4e-156
Identity = 280/296 (94.59%), Postives = 286/296 (96.62%), Query Frame = 0

Query: 10  HNPYTKLLLTSLSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF 69
           HNPYTKLLLT LSAMVAES TFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF
Sbjct: 5   HNPYTKLLLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF 64

Query: 70  GLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGISGSIAQVVAS 129
            LYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASD GSVS H+KALVGGISGSIAQVVAS
Sbjct: 65  ALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDAGSVSFHSKALVGGISGSIAQVVAS 124

Query: 130 PADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNVQRAFLVNMG 189
           PADLVKVRMQADGRL+SQGLQPRYSGP DALTKIVR EG+VGLWKGVVPNVQRAFLVNMG
Sbjct: 125 PADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMG 184

Query: 190 ELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMMNQAASKEGT 249
           ELACYDHAKRFVIQNQIAGDNIFGH+ ASVISGLCATALSCPADVVKTRMMNQAASKEG 
Sbjct: 185 ELACYDHAKRFVIQNQIAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGI 244

Query: 250 IKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLASF 306
            KYNSSYDCL+KTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGL+SF
Sbjct: 245 TKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300

BLAST of Lag0040133 vs. ExPASy TrEMBL
Match: A0A1S3BFM4 (mitochondrial uncoupling protein 3 OS=Cucumis melo OX=3656 GN=LOC103489145 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.4e-156
Identity = 280/296 (94.59%), Postives = 286/296 (96.62%), Query Frame = 0

Query: 10  HNPYTKLLLTSLSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF 69
           HNPYTKLLLT LSAMVAES TFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF
Sbjct: 5   HNPYTKLLLTGLSAMVAESATFPIDLTKTRLQLHGESSSSSRSTNAFRLASAIVKDQGPF 64

Query: 70  GLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGISGSIAQVVAS 129
            LYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASD GSVS H+KALVGGISGSIAQVVAS
Sbjct: 65  ALYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDAGSVSFHSKALVGGISGSIAQVVAS 124

Query: 130 PADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNVQRAFLVNMG 189
           PADLVKVRMQADGRL+SQGLQPRYSGP DALTKIVR EG+VGLWKGVVPNVQRAFLVNMG
Sbjct: 125 PADLVKVRMQADGRLISQGLQPRYSGPFDALTKIVRGEGVVGLWKGVVPNVQRAFLVNMG 184

Query: 190 ELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMMNQAASKEGT 249
           ELACYDHAKRFVIQNQIAGDNIFGH+ ASVISGLCATALSCPADVVKTRMMNQAASKEG 
Sbjct: 185 ELACYDHAKRFVIQNQIAGDNIFGHTCASVISGLCATALSCPADVVKTRMMNQAASKEGI 244

Query: 250 IKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLASF 306
            KYNSSYDCL+KTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGL+SF
Sbjct: 245 TKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLSSF 300

BLAST of Lag0040133 vs. ExPASy TrEMBL
Match: A0A6J1KAM5 (mitochondrial uncoupling protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111491606 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.4e-154
Identity = 276/305 (90.49%), Postives = 291/305 (95.41%), Query Frame = 0

Query: 1   MKSSEEHGRHNPYTKLLLTSLSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLAS 60
           MK+SEEHGRHNP TK+LLT+LSAMVAESTTFPIDLTKTRLQL GESSS S STNAFR+AS
Sbjct: 1   MKTSEEHGRHNPNTKMLLTALSAMVAESTTFPIDLTKTRLQLQGESSSFSSSTNAFRVAS 60

Query: 61  AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGIS 120
           AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHL+SLFL SDGGSVSL AKALVGGIS
Sbjct: 61  AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLQSLFLVSDGGSVSLPAKALVGGIS 120

Query: 121 GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNV 180
           GSIAQVVASPADLVKVRMQADGRLMS+GLQPRYSGP DALTKIVR EGI+GLWKGVVPNV
Sbjct: 121 GSIAQVVASPADLVKVRMQADGRLMSRGLQPRYSGPFDALTKIVRGEGIMGLWKGVVPNV 180

Query: 181 QRAFLVNMGELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMM 240
           QRAFLVNMGEL CYDHAKRFVI+NQIAGDNIFGH+ ASV+SGL ATALSCPADVVKTRMM
Sbjct: 181 QRAFLVNMGELPCYDHAKRFVIRNQIAGDNIFGHTLASVMSGLSATALSCPADVVKTRMM 240

Query: 241 NQAASKEGTIKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA 300
           NQAASKEGT  YN +YDCL+KTVK+EGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA
Sbjct: 241 NQAASKEGTTMYNGTYDCLVKTVKIEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLA 300

Query: 301 GLASF 306
           G++SF
Sbjct: 301 GISSF 305

BLAST of Lag0040133 vs. ExPASy TrEMBL
Match: A0A6J1DAE7 (mitochondrial uncoupling protein 3 OS=Momordica charantia OX=3673 GN=LOC111019146 PE=3 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 3.2e-154
Identity = 278/306 (90.85%), Postives = 294/306 (96.08%), Query Frame = 0

Query: 1   MKSSEEHGRHNPYTKLLLTSLSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFRLAS 60
           MK+S+E+G HN YTKL LTSLSAMVAE+TTFP+DL KTRLQLHGE  SSSRSTNAFR+AS
Sbjct: 1   MKTSKENGGHNSYTKLFLTSLSAMVAETTTFPMDLIKTRLQLHGE--SSSRSTNAFRVAS 60

Query: 61  AIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGIS 120
           +IVK+QGPFGLY+GLSPAILRHLFYTPIRIVGYEHLRSLFLA D GS+SLHAKALVGGIS
Sbjct: 61  SIVKEQGPFGLYRGLSPAILRHLFYTPIRIVGYEHLRSLFLAPDSGSLSLHAKALVGGIS 120

Query: 121 GSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNV 180
           GSIAQVVASPADLVKVRMQADGRL SQGLQPRYSGP DALTKIVRAEGI+GLWKGVVPNV
Sbjct: 121 GSIAQVVASPADLVKVRMQADGRLRSQGLQPRYSGPFDALTKIVRAEGIIGLWKGVVPNV 180

Query: 181 QRAFLVNMGELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMM 240
           QRAFLVNMGELACYDHAKRF+I+NQIAGDNIFGH+FASVISGLCAT LSCPADVVKTRMM
Sbjct: 181 QRAFLVNMGELACYDHAKRFIIENQIAGDNIFGHTFASVISGLCATTLSCPADVVKTRMM 240

Query: 241 NQ-AASKEGTIKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKL 300
           NQ AASKEGTIKYNSSYDCL+KTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKL
Sbjct: 241 NQAAASKEGTIKYNSSYDCLVKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKL 300

Query: 301 AGLASF 306
           AGL+SF
Sbjct: 301 AGLSSF 304

BLAST of Lag0040133 vs. TAIR 10
Match: AT1G14140.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 421.0 bits (1081), Expect = 8.1e-118
Identity = 210/296 (70.95%), Postives = 247/296 (83.45%), Query Frame = 0

Query: 14  TKLLLTSLSAMVAESTTFPIDLTKTRLQLHGE-SSSSSRSTNAFRLASAIVKDQGPFGLY 73
           T++LL SLSAMVAES TFPIDLTKTR+QLHG  S+S +    AF + S I + +G  GLY
Sbjct: 14  TRILLASLSAMVAESVTFPIDLTKTRMQLHGSGSASGAHRIGAFGVVSEIARKEGVIGLY 73

Query: 74  KGLSPAILRHLFYTPIRIVGYEHLRSLFLASD---GGSVSLHAKALVGGISGSIAQVVAS 133
           KGLSPAI+RHLFYTPIRI+GYE+L+ L + S+     S+ L  KALVGG SG IAQVVAS
Sbjct: 74  KGLSPAIIRHLFYTPIRIIGYENLKGLIVRSETNNSESLPLATKALVGGFSGVIAQVVAS 133

Query: 134 PADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNVQRAFLVNMG 193
           PADLVKVRMQADGRL+SQGL+PRYSGP++A TKI+++EG+ GLWKGV+PN+QRAFLVNMG
Sbjct: 134 PADLVKVRMQADGRLVSQGLKPRYSGPIEAFTKILQSEGVKGLWKGVLPNIQRAFLVNMG 193

Query: 194 ELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMMNQAASKEGT 253
           ELACYDHAK FVI  +IA DNIF H+ AS++SGL +T+LSCPADVVKTRMMNQ  +    
Sbjct: 194 ELACYDHAKHFVIDKKIAEDNIFAHTLASIMSGLASTSLSCPADVVKTRMMNQGEN---- 253

Query: 254 IKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKLAGLASF 306
             Y +SYDCL+KTVK EG+RALWKGFFPTWARLGPWQFVFWVSYEKFR LAG++SF
Sbjct: 254 AVYRNSYDCLVKTVKFEGIRALWKGFFPTWARLGPWQFVFWVSYEKFRLLAGISSF 305

BLAST of Lag0040133 vs. TAIR 10
Match: AT5G58970.1 (uncoupling protein 2 )

HSP 1 Score: 188.3 bits (477), Expect = 8.8e-48
Identity = 103/289 (35.64%), Postives = 167/289 (57.79%), Query Frame = 0

Query: 17  LLTSLSAMVAESTTFPIDLTKTRLQLH-----GESSSSSRSTNAFRLASAIVKDQGPFGL 76
           + ++ +A  AE  T P+D  K RLQL      G+  +  +   +    + I +++G  GL
Sbjct: 17  ICSAFAACFAELCTIPLDTAKVRLQLQRKIPTGDGENLPKYRGSIGTLATIAREEGISGL 76

Query: 77  YKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDG-GSVSLHAKALVGGISGSIAQVVASP 136
           +KG+   + R   Y  +RI  YE +++L + SD  G + L+ K L   ++G+IA +VA+P
Sbjct: 77  WKGVIAGLHRQCIYGGLRIGLYEPVKTLLVGSDFIGDIPLYQKILAALLTGAIAIIVANP 136

Query: 137 ADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNVQRAFLVNMGE 196
            DLVKVR+Q++G+L + G+  RY+G +DA   IV+ EG+  LW G+ PN+ R  +VN  E
Sbjct: 137 TDLVKVRLQSEGKLPA-GVPRRYAGAVDAYFTIVKLEGVSALWTGLGPNIARNAIVNAAE 196

Query: 197 LACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMMNQAASKEGTI 256
           LA YD  K  +++     D++  H  A + +G  A  +  P DVVK+RMM       G  
Sbjct: 197 LASYDQIKETIMKIPFFRDSVLTHLLAGLAAGFFAVCIGSPIDVVKSRMM-------GDS 256

Query: 257 KYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKL 300
            Y ++ DC +KT+K EG+ A +KGF P + RLG W  + +++ E+ +K+
Sbjct: 257 TYRNTVDCFIKTMKTEGIMAFYKGFLPNFTRLGTWNAIMFLTLEQVKKV 297

BLAST of Lag0040133 vs. TAIR 10
Match: AT4G24570.1 (dicarboxylate carrier 2 )

HSP 1 Score: 186.0 bits (471), Expect = 4.4e-47
Identity = 108/305 (35.41%), Postives = 162/305 (53.11%), Query Frame = 0

Query: 21  LSAMVAESTTFPIDLTKTRLQLHGESSSSSRST--------------------------N 80
           +++++A  +T P+DL K RLQLHGE+ S++  T                           
Sbjct: 11  IASVIAGCSTHPLDLIKVRLQLHGEAPSTTTVTLLRPALAFPNSSPAAFLETTSSVPKVG 70

Query: 81  AFRLASAIVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKA 140
              L   IVK +G   L+ G+S  +LR   Y+  R+  YE L++ +   + G ++L  K 
Sbjct: 71  PISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKNKWTDPESGKLNLSRKI 130

Query: 141 LVGGISGSIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWK 200
             G ++G I   V +PAD+  VRMQADGRL     +  Y+G  DA+  +V+ EG+  LW+
Sbjct: 131 GAGLVAGGIGAAVGNPADVAMVRMQADGRL-PLAQRRNYAGVGDAIRSMVKGEGVTSLWR 190

Query: 201 GVVPNVQRAFLVNMGELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADV 260
           G    + RA +V   +LA YD  K  +++N +  D +  H  AS  +G  A+  S P DV
Sbjct: 191 GSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASFAAGFVASVASNPVDV 250

Query: 261 VKTRMMNQAASKEGTIKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYE 300
           +KTR+MN          Y+ ++DC +KTVK EG  AL+KGF PT  R GP+  V +V+ E
Sbjct: 251 IKTRVMNMKVG-----AYDGAWDCAVKTVKAEGAMALYKGFVPTVCRQGPFTVVLFVTLE 309

BLAST of Lag0040133 vs. TAIR 10
Match: AT3G54110.1 (plant uncoupling mitochondrial protein 1 )

HSP 1 Score: 183.3 bits (464), Expect = 2.8e-46
Identity = 104/285 (36.49%), Postives = 163/285 (57.19%), Query Frame = 0

Query: 19  TSLSAMVAESTTFPIDLTKTRLQLHGESSSSSRSTNAFR----LASAIVKDQGPFGLYKG 78
           ++ +A V E  T P+D  K RLQL   + +   +   +R        I +++G   L+KG
Sbjct: 18  SAFAACVGEVCTIPLDTAKVRLQLQKSALAGDVTLPKYRGLLGTVGTIAREEGLRSLWKG 77

Query: 79  LSPAILRHLFYTPIRIVGYEHLRSLFLASD-GGSVSLHAKALVGGISGSIAQVVASPADL 138
           + P + R   +  +RI  YE +++L++  D  G V L  K L G  +G++  +VA+P DL
Sbjct: 78  VVPGLHRQCLFGGLRIGMYEPVKNLYVGKDFVGDVPLSKKILAGLTTGALGIMVANPTDL 137

Query: 139 VKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNVQRAFLVNMGELAC 198
           VKVR+QA+G+L + G   RYSG L+A + IVR EG+  LW G+ PNV R  ++N  ELA 
Sbjct: 138 VKVRLQAEGKL-AAGAPRRYSGALNAYSTIVRQEGVRALWTGLGPNVARNAIINAAELAS 197

Query: 199 YDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMMNQAASKEGTIKYN 258
           YD  K  +++     DN+  H  + + +G  A  +  P DVVK+RMM  + + +GTI   
Sbjct: 198 YDQVKETILKIPGFTDNVVTHILSGLGAGFFAVCIGSPVDVVKSRMMGDSGAYKGTI--- 257

Query: 259 SSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRK 299
              DC +KT+K +G  A +KGF P + RLG W  + +++ E+ +K
Sbjct: 258 ---DCFVKTLKSDGPMAFYKGFIPNFGRLGSWNVIMFLTLEQAKK 295

BLAST of Lag0040133 vs. TAIR 10
Match: AT2G22500.1 (uncoupling protein 5 )

HSP 1 Score: 178.7 bits (452), Expect = 7.0e-45
Identity = 102/298 (34.23%), Postives = 163/298 (54.70%), Query Frame = 0

Query: 21  LSAMVAESTTFPIDLTKTRLQLHGESS------------SSSRSTNA-------FRLASA 80
           ++++VA  +T P+DL K R+QL GES+             +S + NA         + S 
Sbjct: 11  IASIVAGCSTHPLDLIKVRMQLQGESAPIQTNLRPALAFQTSTTVNAPPLRVGVIGVGSR 70

Query: 81  IVKDQGPFGLYKGLSPAILRHLFYTPIRIVGYEHLRSLFLASDGGSVSLHAKALVGGISG 140
           +++++G   L+ G+S  +LR   Y+  R+  Y+ ++  +   +  ++ L  K   G I+G
Sbjct: 71  LIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTDPETKTMPLMKKIGAGAIAG 130

Query: 141 SIAQVVASPADLVKVRMQADGRLMSQGLQPRYSGPLDALTKIVRAEGIVGLWKGVVPNVQ 200
           +I   V +PAD+  VRMQADGRL     +  Y   LDA+T+++R EG+  LW+G    + 
Sbjct: 131 AIGAAVGNPADVAMVRMQADGRLPLTD-RRNYKSVLDAITQMIRGEGVTSLWRGSSLTIN 190

Query: 201 RAFLVNMGELACYDHAKRFVIQNQIAGDNIFGHSFASVISGLCATALSCPADVVKTRMMN 260
           RA LV   +LA YD  K  +++  +  D +  H  AS  +G  A+  S P DV+KTR+MN
Sbjct: 191 RAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASFAAGFVASVASNPVDVIKTRVMN 250

Query: 261 QAASKEGTIKYNSSYDCLMKTVKVEGLRALWKGFFPTWARLGPWQFVFWVSYEKFRKL 300
                     Y  + DC +KTVK EG+ +L+KGF PT +R  P+  V +V+ E+ +KL
Sbjct: 251 MKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQAPFTVVLFVTLEQVKKL 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038892574.18.0e-16093.11mitochondrial uncoupling protein 3 [Benincasa hispida][more]
XP_022957541.12.6e-15892.79mitochondrial uncoupling protein 3 [Cucurbita moschata][more]
KAG7032229.15.8e-15892.46Mitochondrial uncoupling protein 3, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_023513662.11.3e-15792.13mitochondrial uncoupling protein 3 [Cucurbita pepo subsp. pepo][more]
XP_004135167.15.4e-15694.26mitochondrial uncoupling protein 3 [Cucumis sativus] >KAE8648752.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
Q9XI741.1e-11670.95Mitochondrial uncoupling protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUMP3 PE=2... [more]
O958477.8e-6543.03Mitochondrial uncoupling protein 4 OS=Homo sapiens OX=9606 GN=SLC25A27 PE=2 SV=1[more]
B0G1434.4e-5239.31Mitochondrial substrate carrier family protein ucpB OS=Dictyostelium discoideum ... [more]
Q6GQ222.1e-4939.44Kidney mitochondrial carrier protein 1 OS=Xenopus laevis OX=8355 GN=slc25a30 PE=... [more]
Q5XGI12.1e-4939.79Kidney mitochondrial carrier protein 1 OS=Xenopus tropicalis OX=8364 GN=slc25a30... [more]
Match NameE-valueIdentityDescription
A0A6J1GZH91.3e-15892.79mitochondrial uncoupling protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111458911... [more]
A0A5A7SUW03.4e-15694.59Mitochondrial uncoupling protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BFM43.4e-15694.59mitochondrial uncoupling protein 3 OS=Cucumis melo OX=3656 GN=LOC103489145 PE=3 ... [more]
A0A6J1KAM52.4e-15490.49mitochondrial uncoupling protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111491606 P... [more]
A0A6J1DAE73.2e-15490.85mitochondrial uncoupling protein 3 OS=Momordica charantia OX=3673 GN=LOC11101914... [more]
Match NameE-valueIdentityDescription
AT1G14140.18.1e-11870.95Mitochondrial substrate carrier family protein [more]
AT5G58970.18.8e-4835.64uncoupling protein 2 [more]
AT4G24570.14.4e-4735.41dicarboxylate carrier 2 [more]
AT3G54110.12.8e-4636.49plant uncoupling mitochondrial protein 1 [more]
AT2G22500.17.0e-4534.23uncoupling protein 5 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023395Mitochondrial carrier domain superfamilyGENE3D1.50.40.10Mitochondrial carrier domaincoord: 14..301
e-value: 2.3E-83
score: 281.4
IPR023395Mitochondrial carrier domain superfamilySUPERFAMILY103506Mitochondrial carriercoord: 9..295
IPR018108Mitochondrial substrate/solute carrierPFAMPF00153Mito_carrcoord: 11..101
e-value: 1.2E-19
score: 70.0
coord: 108..204
e-value: 3.8E-21
score: 74.8
coord: 210..299
e-value: 3.6E-21
score: 74.9
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 14..99
score: 22.427198
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 108..200
score: 27.554489
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 209..299
score: 26.288755
NoneNo IPR availablePANTHERPTHR45618:SF8MITOCHONDRIAL UNCOUPLING PROTEIN 4coord: 4..297
NoneNo IPR availablePANTHERPTHR45618MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATEDcoord: 4..297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040133.1Lag0040133.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane