Lag0040066 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0040066 vs. NCBI nr
Match: XP_038892122.1 (protein NRT1/ PTR FAMILY 2.13-like [Benincasa hispida])

HSP 1 Score: 984.6 bits (2544), Expect = 3.9e-283
Identity = 495/603 (82.09%), Postives = 534/603 (88.56%), Query Frame = 0

Query: 1   MEGQAQSKASSSPSKLHCFTKDADPKLCYSPEINKPKRKPGGWKSMPYILGNETFERLAS 60
           ME +A+SKASSSPS L CFTKD           NK KR+PGGWKSMPYILGNETFERLAS
Sbjct: 15  MESEAESKASSSPSNLQCFTKDVSLH-------NKSKREPGGWKSMPYILGNETFERLAS 74

Query: 61  MGLLANFMVYLRTVYHMDQVSATSLMSVWTGVTNFLPLVGAFLSDAYVGRYWTIAVASIF 120
           MGLLANFMVYL+ VYHMDQVSATSLM +WTGVT+FLPL+GAFLSDAY+GRYWTIAVASIF
Sbjct: 75  MGLLANFMVYLKKVYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAVASIF 134

Query: 121 SLLGMASMTLTAWLPQLHPAECGGGEKCSGPTATHLGFLIMSLCLVSIGSAGIRPCSIPF 180
           S LGM SMTLTAWLPQLHP  C G  KCSGPTA+ LGFLIMSLCLVSIGS GIRPCSIPF
Sbjct: 135 SFLGMTSMTLTAWLPQLHPQGCVGSTKCSGPTASQLGFLIMSLCLVSIGSGGIRPCSIPF 194

Query: 181 GVDQFDPTTEKGRKGIASFYNWYYVSFTVVLVISLTIVVYIQDSVSWVLGYGIPTGLMVC 240
           GVDQFDPTTEKGRKGIASFYNWYY +F VVLV +LTIVVYIQDSVSWVLGYGIPTGLM C
Sbjct: 195 GVDQFDPTTEKGRKGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFC 254

Query: 241 SIVLFFMGTRVYVLMKPEGSIFTGLAQVAVAAYKKRKLQLPSFDAIDRAAFYDPPFDPAT 300
           SIVLFFMGTRVYV +KPEGSIFTGLAQVAVAAYKKR L+LP     DR  FYDPP DP +
Sbjct: 255 SIVLFFMGTRVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPD----DRKGFYDPPLDPDS 314

Query: 301 VVLSKLPLTNQFSFLNKAAMVVEEELNPDGTRINKWNLSTIQQVEEFKCLLRVAPIWVTG 360
           VVLSKLPLTNQFSFLNKAA++ E+E+NPDGTR+NKWN+S+IQQVEE KCL RV PIWVTG
Sbjct: 315 VVLSKLPLTNQFSFLNKAAIITEKEINPDGTRVNKWNVSSIQQVEELKCLFRVFPIWVTG 374

Query: 361 ILSLTPIIQQSTFSISQALQMDRHMGPKFQIPAASIVVISFLTITFFIPLYDRFLVPALR 420
           ILSLTPIIQQSTFSISQALQM+RHMGP FQIP ASI+VISFLTITFFIP YD+FLVP LR
Sbjct: 375 ILSLTPIIQQSTFSISQALQMNRHMGPNFQIPPASIIVISFLTITFFIPFYDQFLVPTLR 434

Query: 421 NLTGHQNGITELQRMAIGIVFAVLSMVAAGLVEMARRNRANGPGGGSRMSVFWLTPQFVL 480
             T H NGITELQRMA+GI+FAV+SMV AGL+EM RRNRAN   GGS MSVFWLTPQF L
Sbjct: 435 KFTRHPNGITELQRMAVGIIFAVVSMVVAGLIEMQRRNRAN--NGGSIMSVFWLTPQFFL 494

Query: 481 LGLCEAFNIIGQIEFFNKEFPEHMRTMGNALSSCSIALASYVSTAMVLIVHRTTGRGGAD 540
           +GLCEAFNIIGQIEFFNKEFPEHMRTMGNA SSCSIAL+SYV+TAMVLIVHRTTG GGA 
Sbjct: 495 MGLCEAFNIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAH 554

Query: 541 GRPDWLADDLNRGRLDYFYYIVAATSFFNFFFFLYCAKNYRYKGKISDSE-DFELV-SSE 600
           GRPDWL DDLN+GRLDYFYY+VAAT+F NFFFFLYCAKNYRYKG +S++E D ELV SS+
Sbjct: 555 GRPDWLVDDLNKGRLDYFYYVVAATAFCNFFFFLYCAKNYRYKGHVSNTERDLELVISSD 604

Query: 601 KKL 602
           KK+
Sbjct: 615 KKI 604

BLAST of Lag0040066 vs. NCBI nr
Match: XP_022998476.1 (protein NRT1/ PTR FAMILY 2.13-like [Cucurbita maxima])

HSP 1 Score: 984.2 bits (2543), Expect = 5.1e-283
Identity = 488/583 (83.70%), Postives = 530/583 (90.91%), Query Frame = 0

Query: 23  ADPKLCYSPEINKPKRKPGGWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVSA 82
           A+PKLC+SP+ NK KR+PGGWKSMPYILGNETFERLASMGLLANFMVYL  VYHMDQVSA
Sbjct: 9   ANPKLCFSPQ-NKAKREPGGWKSMPYILGNETFERLASMGLLANFMVYLTKVYHMDQVSA 68

Query: 83  TSLMSVWTGVTNFLPLVGAFLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAEC 142
           TSLM +WTGVTNFLPL+GAFLSDAY GRYWTIAVAS FS LGM SMTLTAWLPQLHP  C
Sbjct: 69  TSLMGLWTGVTNFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPTC 128

Query: 143 GGGEKCSGPTATHLGFLIMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYNW 202
            G   C GPTA+ LGFL+MSLCLVSIGSAGIRPCSIPFGVDQFDPTT+KGRKGIASFYNW
Sbjct: 129 SGA-NCEGPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYNW 188

Query: 203 YYVSFTVVLVISLTIVVYIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSIF 262
           YY +F+VVLV +LTIVVYIQDSVSWVLGYGIPT LM CSIVLFFMGT VYV +KPEGSIF
Sbjct: 189 YYATFSVVLVFTLTIVVYIQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSIF 248

Query: 263 TGLAQVAVAAYKKRKLQLPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMVV 322
           TGLAQVAVAAYKKR L+LPS D  DR AFYDPP +PA+VVLSKLPLTNQFSFL KAA+VV
Sbjct: 249 TGLAQVAVAAYKKRHLELPSLD--DRTAFYDPPLEPASVVLSKLPLTNQFSFLTKAAIVV 308

Query: 323 EEELNPDGTRINKWNLSTIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQMD 382
           E+ELNPDGTRINKWNLS+IQQVEE KCL+RV PIWVTGILSLTPIIQQ+TFSISQALQMD
Sbjct: 309 EKELNPDGTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQMD 368

Query: 383 RHMGPKFQIPAASIVVISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVFA 442
           RHMGP FQ+P ASI+VISFLTITFFIPLYD+FLVPA R  T H NGITELQRMA+GI+FA
Sbjct: 369 RHMGPNFQMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTQHPNGITELQRMAVGIIFA 428

Query: 443 VLSMVAAGLVEMARRNRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEFPE 502
           VLSM+ AG+VE  RRNRANGP GGS MSVFWLTPQF L+GLCEAFNIIGQIEFFNKEFPE
Sbjct: 429 VLSMIVAGIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPE 488

Query: 503 HMRTMGNALSSCSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYYIV 562
           HMRTMGNA SSCSIAL+SYV+TAMVLIVHRTTG GGA G+PDWL +DLN+GRLDYFYY+V
Sbjct: 489 HMRTMGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLTNDLNKGRLDYFYYVV 548

Query: 563 AATSFFNFFFFLYCAKNYRYKGKISDS-EDFELV-SSEKKLEV 604
           AAT+FFNFFFF+YCAKNY YKGK+SDS +D ELV +S+KKL+V
Sbjct: 549 AATAFFNFFFFIYCAKNYHYKGKVSDSQQDLELVIASDKKLDV 587

BLAST of Lag0040066 vs. NCBI nr
Match: KAG6601403.1 (Protein NRT1/ PTR FAMILY 2.13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 980.3 bits (2533), Expect = 7.4e-282
Identity = 485/584 (83.05%), Postives = 530/584 (90.75%), Query Frame = 0

Query: 22  DADPKLCYSPEINKPKRKPGGWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVS 81
           DA+ ++  +P++ K +R+PGGWKSMPYILGNETFERLASMGLLANFMVYLR VYHMDQVS
Sbjct: 2   DAEHQIKANPKLRKSRREPGGWKSMPYILGNETFERLASMGLLANFMVYLRKVYHMDQVS 61

Query: 82  ATSLMSVWTGVTNFLPLVGAFLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAE 141
           ATSLM +WTGVTNFLPL+GAFLSDAY GRYWTIAVAS FS LGM SMTLTAWLPQLHP  
Sbjct: 62  ATSLMGLWTGVTNFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPT 121

Query: 142 CGGGEKCSGPTATHLGFLIMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYN 201
           C G   C GPTA+ LGFL+MSLCLVSIGSAGIRPCSIPFGVDQFDPTT+KGRKGIASFYN
Sbjct: 122 CSGA-NCEGPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYN 181

Query: 202 WYYVSFTVVLVISLTIVVYIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSI 261
           WYY +F+VVLV +LTIVVYIQDSVSWVLGYGIPT LM CSIVLFFMGT VYV +KPEGSI
Sbjct: 182 WYYATFSVVLVFTLTIVVYIQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSI 241

Query: 262 FTGLAQVAVAAYKKRKLQLPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMV 321
           FTGLAQVAVAAYKKR L+LPS D  DR AFYDPP +P +VVLSKLPLTNQFSFLNKAA+V
Sbjct: 242 FTGLAQVAVAAYKKRHLELPSLD--DRTAFYDPPLEPGSVVLSKLPLTNQFSFLNKAAIV 301

Query: 322 VEEELNPDGTRINKWNLSTIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQM 381
           VE+ELNPDGTRINKWNLS+IQQVEE KCL+RV PIWVTGILSLTPIIQQ+TFSISQALQM
Sbjct: 302 VEKELNPDGTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQM 361

Query: 382 DRHMGPKFQIPAASIVVISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVF 441
           DRHMGP FQ+P ASI+VISFLTITFFIPLYD+FLVPA R  T H NGITELQRMAIGI+F
Sbjct: 362 DRHMGPNFQMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTHHPNGITELQRMAIGIIF 421

Query: 442 AVLSMVAAGLVEMARRNRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEFP 501
           AVLSM+ AG+VE  RRNRANGP GGS MSVFWLTPQF L+GLCEAFNIIGQIEFFNKEFP
Sbjct: 422 AVLSMIVAGIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFP 481

Query: 502 EHMRTMGNALSSCSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYYI 561
           EHMRTMGNA SSCSIAL+SYV+TAMVLIVHRTTG GGA G+PDWLA+DLN+GRLDYFYY+
Sbjct: 482 EHMRTMGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLANDLNKGRLDYFYYV 541

Query: 562 VAATSFFNFFFFLYCAKNYRYKGKISDS-EDFELV-SSEKKLEV 604
           VAAT+FFNFFFF+YCAKNY YKGK+SDS +D ELV +S+KKL+V
Sbjct: 542 VAATAFFNFFFFIYCAKNYHYKGKVSDSHQDLELVIASDKKLDV 582

BLAST of Lag0040066 vs. NCBI nr
Match: KAG7032183.1 (Protein NRT1/ PTR FAMILY 2.13 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 979.9 bits (2532), Expect = 9.6e-282
Identity = 484/584 (82.88%), Postives = 530/584 (90.75%), Query Frame = 0

Query: 22  DADPKLCYSPEINKPKRKPGGWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVS 81
           DA+ ++  +P++ K +R+PGGWKSMPYILGNETFERLASMGLLANFMVYLR VYHMDQVS
Sbjct: 2   DAEHQIKANPKLRKSRREPGGWKSMPYILGNETFERLASMGLLANFMVYLRKVYHMDQVS 61

Query: 82  ATSLMSVWTGVTNFLPLVGAFLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAE 141
           ATSLM +WTGVTNFLPL+GAFLSDAY GRYWTIAVAS FS LGM SMTLTAWLPQLHP  
Sbjct: 62  ATSLMGLWTGVTNFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPT 121

Query: 142 CGGGEKCSGPTATHLGFLIMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYN 201
           C G   C GPTA+ LGFL+MSLCLVSIGSAGIRPCSIPFGVDQFDPTT+KGRKGIASFYN
Sbjct: 122 CSGA-NCEGPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYN 181

Query: 202 WYYVSFTVVLVISLTIVVYIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSI 261
           WYY +F+VVLV +LTIVVY+QDSVSWVLGYGIPT LM CSIVLFFMGT VYV +KPEGSI
Sbjct: 182 WYYATFSVVLVFTLTIVVYVQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSI 241

Query: 262 FTGLAQVAVAAYKKRKLQLPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMV 321
           FTGLAQVAVAAYKKR L+LPS D  DR AFYDPP +P +VVLSKLPLTNQFSFLNKAA+V
Sbjct: 242 FTGLAQVAVAAYKKRHLELPSLD--DRTAFYDPPLEPGSVVLSKLPLTNQFSFLNKAAIV 301

Query: 322 VEEELNPDGTRINKWNLSTIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQM 381
           VE+ELNPDGTRINKWNLS+IQQVEE KCL+RV PIWVTGILSLTPIIQQ+TFSISQALQM
Sbjct: 302 VEKELNPDGTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQM 361

Query: 382 DRHMGPKFQIPAASIVVISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVF 441
           DRHMGP FQ+P ASI+VISFLTITFFIPLYD+FLVPA R  T H NGITELQRMAIGI+F
Sbjct: 362 DRHMGPNFQMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTHHPNGITELQRMAIGIIF 421

Query: 442 AVLSMVAAGLVEMARRNRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEFP 501
           AVLSM+ AG+VE  RRNRANGP GGS MSVFWLTPQF L+GLCEAFNIIGQIEFFNKEFP
Sbjct: 422 AVLSMIVAGIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFP 481

Query: 502 EHMRTMGNALSSCSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYYI 561
           EHMRTMGNA SSCSIAL+SYV+TAMVLIVHRTTG GGA G+PDWLA+DLN+GRLDYFYY+
Sbjct: 482 EHMRTMGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLANDLNKGRLDYFYYV 541

Query: 562 VAATSFFNFFFFLYCAKNYRYKGKISDS-EDFELV-SSEKKLEV 604
           VAAT+FFNFFFF+YCAKNY YKGK+SDS +D ELV +S+KKL+V
Sbjct: 542 VAATAFFNFFFFIYCAKNYHYKGKVSDSHQDLELVIASDKKLDV 582

BLAST of Lag0040066 vs. NCBI nr
Match: XP_022957160.1 (protein NRT1/ PTR FAMILY 2.13-like [Cucurbita moschata])

HSP 1 Score: 979.9 bits (2532), Expect = 9.6e-282
Identity = 484/584 (82.88%), Postives = 530/584 (90.75%), Query Frame = 0

Query: 22  DADPKLCYSPEINKPKRKPGGWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVS 81
           DA+ ++  +P++ K +R+PGGWKSMPYILGNETFERLASMGLLANFMVYLR VYHMDQVS
Sbjct: 2   DAEHQIKANPKLRKSRREPGGWKSMPYILGNETFERLASMGLLANFMVYLRKVYHMDQVS 61

Query: 82  ATSLMSVWTGVTNFLPLVGAFLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAE 141
           ATSLM +WTGVTNFLPL+GAFLSDAY GRYWTIAVAS FS LGM SMTLTAWLPQLHP  
Sbjct: 62  ATSLMGLWTGVTNFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPT 121

Query: 142 CGGGEKCSGPTATHLGFLIMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYN 201
           C G   C GPTA+ LGFL+MSLCLVSIGSAGIRPCSIPFGVDQFDPTT+KGRKGIASFYN
Sbjct: 122 CSGA-NCEGPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYN 181

Query: 202 WYYVSFTVVLVISLTIVVYIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSI 261
           WYY +F+VVLV +LTIVVYIQDSVSWVLGYGIPT LM CSIVLFFMGT VYV +KPEGSI
Sbjct: 182 WYYATFSVVLVFTLTIVVYIQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSI 241

Query: 262 FTGLAQVAVAAYKKRKLQLPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMV 321
           FTGLAQVAVAAYKKR L+LPS D  DR AFYDPP +P +VVLSKLPLTNQFSFLNKAA+V
Sbjct: 242 FTGLAQVAVAAYKKRHLELPSLD--DRTAFYDPPLEPGSVVLSKLPLTNQFSFLNKAAIV 301

Query: 322 VEEELNPDGTRINKWNLSTIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQM 381
           VE+ELNPDGTRINKWNLS+IQQVEE KCL+RV PIWVTGILSLTPIIQQ+TFSISQALQM
Sbjct: 302 VEKELNPDGTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQM 361

Query: 382 DRHMGPKFQIPAASIVVISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVF 441
           DRHMGP FQ+P ASI+VISFLTITFFIPLYD+FLVPA R  T H NGITELQRMA+GI+F
Sbjct: 362 DRHMGPNFQMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTHHPNGITELQRMAVGIIF 421

Query: 442 AVLSMVAAGLVEMARRNRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEFP 501
           AVLSM+ AG+VE  RRNRANGP GGS MSVFWLTPQF L+GLCEAFNIIGQIEFFNKEFP
Sbjct: 422 AVLSMIVAGIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFP 481

Query: 502 EHMRTMGNALSSCSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYYI 561
           EHMRTMGNA SSCSIAL+SYV+TAMVLIVHRTTG GGA G+PDWLA+DLN+GRLDYFYY+
Sbjct: 482 EHMRTMGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLANDLNKGRLDYFYYV 541

Query: 562 VAATSFFNFFFFLYCAKNYRYKGKISDS-EDFELV-SSEKKLEV 604
           VAAT+FFNFFFF+YCAKNY YKGK+SDS +D ELV +S+KKL+V
Sbjct: 542 VAATAFFNFFFFIYCAKNYHYKGKVSDSQQDLELVIASDKKLDV 582

BLAST of Lag0040066 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 1.9e-187
Identity = 336/613 (54.81%), Postives = 447/613 (72.92%), Query Frame = 0

Query: 1   MEGQAQSKASSSPSKLHCFTKDADP-KLCYSP----EINKPKRKPGGWKSMPYILGNETF 60
           + G++ S + SSPS+L       DP K   SP    +  K ++KPGGW+++ +ILGNET 
Sbjct: 12  LPGRSGSFSKSSPSELDV----VDPYKRISSPGSILDAEKVEKKPGGWRAVSFILGNETL 71

Query: 61  ERLASMGLLANFMVYLRTVYHMDQVSATSLMSVWTGVTNFLPLVGAFLSDAYVGRYWTIA 120
           ERL S+GLLANFMVYL  V+H++QV A +++++W+G TN  PLVGA++SD YVGR+ TIA
Sbjct: 72  ERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVGAYISDTYVGRFKTIA 131

Query: 121 VASIFSLLGMASMTLTAWLPQLHPAECGGGE--KCSGPTATHLGFLIMSLCLVSIGSAGI 180
            AS  +LLG+ ++TLTA  PQLHPA C   +   C GP    +G L++ LC +S+GS GI
Sbjct: 132 FASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVLLLGLCFLSVGSGGI 191

Query: 181 RPCSIPFGVDQFDPTTEKGRKGIASFYNWYYVSFTVVLVISLTIVVYIQDSVSWVLGYGI 240
           RPCSIPFGVDQFD  TE+G KG+ASF+NWYY++FTVVL+I+ T+VVYIQD VSW++G+ I
Sbjct: 192 RPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVVVYIQDQVSWIIGFSI 251

Query: 241 PTGLMVCSIVLFFMGTRVYVLMKPEGSIFTGLAQVAVAAYKKRKLQLPSFDAIDRAAFYD 300
           PTGLM  ++V+FF G + YV +KPEGSIF+G+AQV VAA KKRKL+LP+ D      +YD
Sbjct: 252 PTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLKLPAEDD-GTVTYYD 311

Query: 301 PPFDPATVVLSKLPLTNQFSFLNKAAMVVEEELNPDGTRINKWNLSTIQQVEEFKCLLRV 360
           P    +  VLSKL  +NQF  L+KAA+V+E +L P+G   +KW L ++Q+VEE KCL+R+
Sbjct: 312 PAIKSS--VLSKLHRSNQFRCLDKAAVVIEGDLTPEGPPADKWRLCSVQEVEEVKCLIRI 371

Query: 361 APIWVTGILSLTPIIQQSTFSISQALQMDRHMGPKFQIPAASIVVISFLTITFFIPLYDR 420
            PIW  GI+SL  +  Q TF++SQAL+MDR++GPKF+IPA S+ VIS LTI  F+P YDR
Sbjct: 372 VPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVISLLTIGIFLPFYDR 431

Query: 421 FLVPALRNLTGHQNGITELQRMAIGIVFAVLSMVAAGLVEMARRNR---ANGPGGGSRMS 480
             VP +R +TGH++GIT LQR+  GIVFA+ SM+ AG+VE  RR R   A  P G + MS
Sbjct: 432 VFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIRSINAGDPTGMTPMS 491

Query: 481 VFWLTPQFVLLGLCEAFNIIGQIEFFNKEFPEHMRTMGNALSSCSIALASYVSTAMVLIV 540
           VFWL+PQ +L+GLCEAFNIIGQIEFFN +FPEHMR++ N+L S S A +SY+S+ +V +V
Sbjct: 492 VFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSFAGSSYLSSFLVTVV 551

Query: 541 HRTTGRGGADGRPDWLADDLNRGRLDYFYYIVAATSFFNFFFFLYCAKNYRYKGKISDSE 600
           H+ +  GG D RPDWL  +LN G+LDYFYY++A     N  +F YCA+ YRYK  +   E
Sbjct: 552 HKFS--GGHD-RPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFWYCARGYRYKVGL-PIE 611

Query: 601 DFELVSSEKKLEV 604
           DFE   S   +E+
Sbjct: 612 DFEEDKSSDDVEM 613

BLAST of Lag0040066 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 599.4 bits (1544), Expect = 4.6e-170
Identity = 303/568 (53.35%), Postives = 407/568 (71.65%), Query Frame = 0

Query: 36  PKRKPGGWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVSATSLMSVWTGVTNF 95
           P++K GGW+++ +ILGNET E+L S+G+ ANFM+YLR V+HM+ V A ++  +W G+TNF
Sbjct: 11  PEKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNF 70

Query: 96  LPLVGAFLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAECGG--GEKCSGPTA 155
            PL+GA +SDAY+GR+ TIA AS+FS+LG+ ++TLTA LPQLHP  C     ++C  P  
Sbjct: 71  APLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNK 130

Query: 156 THLGFLIMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYVSFTVVLVI 215
             LG L + L  +SIGS GIRPCSIPFGVDQFD  TE+G KG+ASF+NWYY++ T+VL+ 
Sbjct: 131 LQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIF 190

Query: 216 SLTIVVYIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSIFTGLAQVAVAAY 275
           S T+VVY+Q +VSWV+G+ IPT LM C++VLFF+G R YV +KPEGS+F+G+A+V VAA 
Sbjct: 191 SHTVVVYLQ-TVSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAAR 250

Query: 276 KKRKLQLPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMVVEEELNPDGTRI 335
           KKR L++   D      +Y+PP  P   VLSKLPLT+QF FL+KAA++++ +L  +G   
Sbjct: 251 KKRDLKISLVDD-GTEEYYEPPVKPG--VLSKLPLTDQFKFLDKAAVILDGDLTSEGVPA 310

Query: 336 NKWNLSTIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQMDRHMGPKFQIPA 395
           NKW L +IQ+VEE KCL+RV P+W  GI+S+  +  Q+TF + QA +MDRHMGP F+IPA
Sbjct: 311 NKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPA 370

Query: 396 ASIVVISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVFAVLSMVAAGLVE 455
           ASI VIS++TI  ++P+Y+  LVP L  +   +  +T LQRM IGIVFA+LSM  AG VE
Sbjct: 371 ASITVISYITIGIWVPIYEHLLVPFLWRMRKFR--VTLLQRMGIGIVFAILSMFTAGFVE 430

Query: 456 MARRNRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEFPEHMRTMGNALSS 515
             RR RA      ++MSVFWL    +L+GLCE+FN IG IEFFN +FPEHMR++ N+L  
Sbjct: 431 GVRRTRATEM---TQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFP 490

Query: 516 CSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYYIVAATSFFNFFFF 575
            S A A+Y+S+ +V  VH+ +   G    PDWL  DL+RG+LDYFYY++A     N  +F
Sbjct: 491 LSFAAANYLSSLLVTTVHKVS---GTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVNLVYF 550

Query: 576 LYCAKNYRYKGKISDSEDFELVSSEKKL 602
            YCA  Y+YK   S  EDF   + EK L
Sbjct: 551 WYCAHRYQYKAG-SQIEDF---NEEKSL 562

BLAST of Lag0040066 vs. ExPASy Swiss-Prot
Match: Q9CAR9 (Putative protein NRT1/ PTR FAMILY 2.14 OS=Arabidopsis thaliana OX=3702 GN=NPF2.14 PE=5 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 7.7e-141
Identity = 256/558 (45.88%), Postives = 376/558 (67.38%), Query Frame = 0

Query: 30  SPEINKPKRKPGGWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVSATSLMSVW 89
           S ++   +RKP GWK+MPYI+GNET ERLA+ GL+ANFMVY+   YHMDQV A +L++ W
Sbjct: 9   SSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINTW 68

Query: 90  TGVTNFLPLVGAFLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAECGGGE--- 149
           + +TNF P++GAF+SD+Y G++ TI   SI  LLGM  +T T+ +P L P  C   +   
Sbjct: 69  SALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPCTADQITG 128

Query: 150 KCSGPTATHLGFLIMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYVS 209
           +C   + + L  L+  L L+S+G+ GIR CS+PF +DQFD +TE+GR+G  SF++WYY +
Sbjct: 129 QCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYYTT 188

Query: 210 FTVVLVISLTIVVYIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSIFTGLA 269
            T+V ++S+T+V+Y+Q+++SW +G+ IPT L   +++L F+GTR YV +KPEGS+F+G+ 
Sbjct: 189 HTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSGVF 248

Query: 270 QVAVAAYKKRKLQLPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMVVEEEL 329
           +V VAAYKKRK +  S   ID    Y  P     +  +KL LT+QF FLNKA +V    +
Sbjct: 249 KVLVAAYKKRKARFTS--GID----YHQPLLETDLQSNKLVLTDQFRFLNKAVIV----M 308

Query: 330 NPDGTRINKWNLSTIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQMD-RHM 389
           N D     +W   T++Q+E+ K ++ + PI+ + I+    + QQ TF++SQAL+MD +  
Sbjct: 309 NNDEAGNEEWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFP 368

Query: 390 GPKFQIPAASIVVISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVFAVLS 449
           G  + IP ASI VIS L I  ++P Y+  LV  + N+T    GI+ LQ++ IG +F++ +
Sbjct: 369 GTSYLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSIST 428

Query: 450 MVAAGLVEMARRNRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEFPEHMR 509
           M+ +G+VE  RR+ +     G +MSVFWLTPQ VL+G  + F I+G  EFFNK+ P +MR
Sbjct: 429 MLISGIVERKRRDLSL---NGVKMSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMR 488

Query: 510 TMGNALSSCSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYYIVAAT 569
           ++GN+L    ++LASY+S+AMV IVH  T RGG      WL DD+++ +LD FYY +AA 
Sbjct: 489 SIGNSLLYLGLSLASYLSSAMVSIVHSVTARGG----QSWLTDDIDKSKLDCFYYFIAAL 548

Query: 570 SFFNFFFFLYCAKNYRYK 584
           S  NF FF +CA+ YRY+
Sbjct: 549 STLNFIFFFWCARRYRYR 549

BLAST of Lag0040066 vs. ExPASy Swiss-Prot
Match: Q9M9V7 (Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 4.2e-131
Identity = 246/570 (43.16%), Postives = 364/570 (63.86%), Query Frame = 0

Query: 42  GWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVSATSLMSVWTGVTNFLPLVGA 101
           GWK MP+I+GNETFE+L  +G  +N ++YL TV++M  ++A  +++++ G +NF  +V A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 102 FLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAECGG--GEKCSGPTATHLGFL 161
           FL D+Y GRY T++ A I   LG  +M LTA +  LHPA+C    G  C+GP+   + FL
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNGPSIGQIMFL 141

Query: 162 IMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYVSFTVVLVISLTIVV 221
             ++ L+ IG+ GIRPC++PFG DQFDP T++G++GI SF+NWY+ +FT   ++SLT++V
Sbjct: 142 AGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIESFFNWYFFTFTFAQMVSLTLIV 201

Query: 222 YIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSIFTGLAQVAVAAYKKRKLQ 281
           Y+Q +VSW +G  IP  LM+   ++FF G+++YV +K  GS    + +V V A KKR+L+
Sbjct: 202 YVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRLK 261

Query: 282 LPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMVVEEE-LNPDGTRINKWNL 341
                 +     Y+  +  +    SKL  T QF FL+K+A+  +++ LN DG+ ++ W L
Sbjct: 262 -----PVGPNELYN--YIASDFKNSKLGHTEQFRFLDKSAIQTQDDKLNKDGSPVDAWKL 321

Query: 342 STIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQMDRHMGP-KFQIPAASIV 401
            ++QQVEE KC++RV P+W++  L     IQQ+T++I Q+LQ DR +GP  FQIPA S  
Sbjct: 322 CSMQQVEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYT 381

Query: 402 VISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVFAVLSMVAAGLVEMARR 461
           V   L +T FIP+YDR LVP LR  TG   GIT+LQR+  G+   + SM+ + +VE  RR
Sbjct: 382 VFLMLGMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRR 441

Query: 462 ---------NRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEFPEHMRTMG 521
                      A   G  S MS  WL PQ VL+G+ +A   +GQ+EF+ K+FPE+MR+  
Sbjct: 442 KVALTKPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFA 501

Query: 522 NALSSCSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYYIVAATSFF 581
            +L  C I LASY+ST ++  VH TT  G + G   WL +DLN+GRL+YFY++VA     
Sbjct: 502 GSLYYCGIGLASYLSTFLLSAVHDTT-EGFSGG--SWLPEDLNKGRLEYFYFLVAGMMTL 561

Query: 582 NFFFFLYCAKNYRYKGKISDSEDFELVSSE 599
           N  +FL  +  YRYK  ++  +D +  S+E
Sbjct: 562 NLAYFLLVSHWYRYKDVVAKDKDMDKTSAE 581

BLAST of Lag0040066 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 1.8e-129
Identity = 250/573 (43.63%), Postives = 352/573 (61.43%), Query Frame = 0

Query: 34  NKPKRKPGGWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVSATSLMSVWTGVT 93
           N+ K    GWK MP+I+GNETFE+L  +G L+N +VYL  V+++  ++A ++++ ++G  
Sbjct: 38  NQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTI 97

Query: 94  NFLPLVGAFLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAECG--GGEKCSGP 153
           NF   V AFL D Y GRY T++VA I   LG   + LTA +PQLHPA CG      C+GP
Sbjct: 98  NFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGP 157

Query: 154 TATHLGFLIMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYVSFTVVL 213
           +   + FL+M L  + +G+ GIRPC++ FG DQF+P +E G++GI SF+NWY+ +FT   
Sbjct: 158 SGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQ 217

Query: 214 VISLTIVVYIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSIFTGLAQVAVA 273
           ++SLT+VVY+Q +VSW +G  IP  LM  + ++FF G ++YV +K  GS   G+AQV   
Sbjct: 218 ILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAV 277

Query: 274 AYKKRKLQLPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMVV-EEELNPDG 333
           A KKR L+      ++   +Y P +       SKL  T+QF FL+KAA++  E++L PDG
Sbjct: 278 AIKKRGLKPAKQPWLNLYNYYPPKY-----ANSKLKYTDQFRFLDKAAILTPEDKLQPDG 337

Query: 334 TRINKWNLSTIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQMDRHMGP-KF 393
              + W L T+QQVEE KC++RV PIW    +    I QQ T+ + QALQ DR +G   F
Sbjct: 338 KPADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGF 397

Query: 394 QIPAASIVVISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVFAVLSMVAA 453
            IPAA+ VV     +T FI +YDR LVP +R +TG   GIT LQR+  GI FA  S+V A
Sbjct: 398 VIPAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVA 457

Query: 454 GLVEMARR---------NRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEF 513
           G VE  RR           A   G  S MS  WL PQ  L G+ EAF  IGQ+EF+ K+F
Sbjct: 458 GFVEERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQF 517

Query: 514 PEHMRTMGNALSSCSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYY 573
           PE+MR+   ++      ++SY+ + ++  VHRTT         +WLA+DLN+GRLD FY+
Sbjct: 518 PENMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGG---NWLAEDLNKGRLDLFYF 577

Query: 574 IVAATSFFNFFFFLYCAKNYRYKGKISDSEDFE 594
           ++A     NF +FL  ++ YRYKG   +   +E
Sbjct: 578 MIAGILAVNFAYFLVMSRWYRYKGSDDEVTTYE 602

BLAST of Lag0040066 vs. ExPASy TrEMBL
Match: A0A6J1KAA8 (protein NRT1/ PTR FAMILY 2.13-like OS=Cucurbita maxima OX=3661 GN=LOC111493070 PE=3 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 2.5e-283
Identity = 488/583 (83.70%), Postives = 530/583 (90.91%), Query Frame = 0

Query: 23  ADPKLCYSPEINKPKRKPGGWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVSA 82
           A+PKLC+SP+ NK KR+PGGWKSMPYILGNETFERLASMGLLANFMVYL  VYHMDQVSA
Sbjct: 9   ANPKLCFSPQ-NKAKREPGGWKSMPYILGNETFERLASMGLLANFMVYLTKVYHMDQVSA 68

Query: 83  TSLMSVWTGVTNFLPLVGAFLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAEC 142
           TSLM +WTGVTNFLPL+GAFLSDAY GRYWTIAVAS FS LGM SMTLTAWLPQLHP  C
Sbjct: 69  TSLMGLWTGVTNFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPTC 128

Query: 143 GGGEKCSGPTATHLGFLIMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYNW 202
            G   C GPTA+ LGFL+MSLCLVSIGSAGIRPCSIPFGVDQFDPTT+KGRKGIASFYNW
Sbjct: 129 SGA-NCEGPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYNW 188

Query: 203 YYVSFTVVLVISLTIVVYIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSIF 262
           YY +F+VVLV +LTIVVYIQDSVSWVLGYGIPT LM CSIVLFFMGT VYV +KPEGSIF
Sbjct: 189 YYATFSVVLVFTLTIVVYIQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSIF 248

Query: 263 TGLAQVAVAAYKKRKLQLPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMVV 322
           TGLAQVAVAAYKKR L+LPS D  DR AFYDPP +PA+VVLSKLPLTNQFSFL KAA+VV
Sbjct: 249 TGLAQVAVAAYKKRHLELPSLD--DRTAFYDPPLEPASVVLSKLPLTNQFSFLTKAAIVV 308

Query: 323 EEELNPDGTRINKWNLSTIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQMD 382
           E+ELNPDGTRINKWNLS+IQQVEE KCL+RV PIWVTGILSLTPIIQQ+TFSISQALQMD
Sbjct: 309 EKELNPDGTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQMD 368

Query: 383 RHMGPKFQIPAASIVVISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVFA 442
           RHMGP FQ+P ASI+VISFLTITFFIPLYD+FLVPA R  T H NGITELQRMA+GI+FA
Sbjct: 369 RHMGPNFQMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTQHPNGITELQRMAVGIIFA 428

Query: 443 VLSMVAAGLVEMARRNRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEFPE 502
           VLSM+ AG+VE  RRNRANGP GGS MSVFWLTPQF L+GLCEAFNIIGQIEFFNKEFPE
Sbjct: 429 VLSMIVAGIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFPE 488

Query: 503 HMRTMGNALSSCSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYYIV 562
           HMRTMGNA SSCSIAL+SYV+TAMVLIVHRTTG GGA G+PDWL +DLN+GRLDYFYY+V
Sbjct: 489 HMRTMGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLTNDLNKGRLDYFYYVV 548

Query: 563 AATSFFNFFFFLYCAKNYRYKGKISDS-EDFELV-SSEKKLEV 604
           AAT+FFNFFFF+YCAKNY YKGK+SDS +D ELV +S+KKL+V
Sbjct: 549 AATAFFNFFFFIYCAKNYHYKGKVSDSQQDLELVIASDKKLDV 587

BLAST of Lag0040066 vs. ExPASy TrEMBL
Match: A0A6J1GZR8 (protein NRT1/ PTR FAMILY 2.13-like OS=Cucurbita moschata OX=3662 GN=LOC111458629 PE=3 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 4.7e-282
Identity = 484/584 (82.88%), Postives = 530/584 (90.75%), Query Frame = 0

Query: 22  DADPKLCYSPEINKPKRKPGGWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVS 81
           DA+ ++  +P++ K +R+PGGWKSMPYILGNETFERLASMGLLANFMVYLR VYHMDQVS
Sbjct: 2   DAEHQIKANPKLRKSRREPGGWKSMPYILGNETFERLASMGLLANFMVYLRKVYHMDQVS 61

Query: 82  ATSLMSVWTGVTNFLPLVGAFLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAE 141
           ATSLM +WTGVTNFLPL+GAFLSDAY GRYWTIAVAS FS LGM SMTLTAWLPQLHP  
Sbjct: 62  ATSLMGLWTGVTNFLPLLGAFLSDAYAGRYWTIAVASFFSFLGMTSMTLTAWLPQLHPPT 121

Query: 142 CGGGEKCSGPTATHLGFLIMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYN 201
           C G   C GPTA+ LGFL+MSLCLVSIGSAGIRPCSIPFGVDQFDPTT+KGRKGIASFYN
Sbjct: 122 CSGA-NCEGPTASQLGFLVMSLCLVSIGSAGIRPCSIPFGVDQFDPTTDKGRKGIASFYN 181

Query: 202 WYYVSFTVVLVISLTIVVYIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSI 261
           WYY +F+VVLV +LTIVVYIQDSVSWVLGYGIPT LM CSIVLFFMGT VYV +KPEGSI
Sbjct: 182 WYYATFSVVLVFTLTIVVYIQDSVSWVLGYGIPTVLMFCSIVLFFMGTHVYVFIKPEGSI 241

Query: 262 FTGLAQVAVAAYKKRKLQLPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMV 321
           FTGLAQVAVAAYKKR L+LPS D  DR AFYDPP +P +VVLSKLPLTNQFSFLNKAA+V
Sbjct: 242 FTGLAQVAVAAYKKRHLELPSLD--DRTAFYDPPLEPGSVVLSKLPLTNQFSFLNKAAIV 301

Query: 322 VEEELNPDGTRINKWNLSTIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQM 381
           VE+ELNPDGTRINKWNLS+IQQVEE KCL+RV PIWVTGILSLTPIIQQ+TFSISQALQM
Sbjct: 302 VEKELNPDGTRINKWNLSSIQQVEELKCLVRVGPIWVTGILSLTPIIQQATFSISQALQM 361

Query: 382 DRHMGPKFQIPAASIVVISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVF 441
           DRHMGP FQ+P ASI+VISFLTITFFIPLYD+FLVPA R  T H NGITELQRMA+GI+F
Sbjct: 362 DRHMGPNFQMPPASIIVISFLTITFFIPLYDQFLVPAFRKFTHHPNGITELQRMAVGIIF 421

Query: 442 AVLSMVAAGLVEMARRNRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEFP 501
           AVLSM+ AG+VE  RRNRANGP GGS MSVFWLTPQF L+GLCEAFNIIGQIEFFNKEFP
Sbjct: 422 AVLSMIVAGIVEKERRNRANGPNGGSIMSVFWLTPQFFLMGLCEAFNIIGQIEFFNKEFP 481

Query: 502 EHMRTMGNALSSCSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYYI 561
           EHMRTMGNA SSCSIAL+SYV+TAMVLIVHRTTG GGA G+PDWLA+DLN+GRLDYFYY+
Sbjct: 482 EHMRTMGNAFSSCSIALSSYVNTAMVLIVHRTTGSGGAHGKPDWLANDLNKGRLDYFYYV 541

Query: 562 VAATSFFNFFFFLYCAKNYRYKGKISDS-EDFELV-SSEKKLEV 604
           VAAT+FFNFFFF+YCAKNY YKGK+SDS +D ELV +S+KKL+V
Sbjct: 542 VAATAFFNFFFFIYCAKNYHYKGKVSDSQQDLELVIASDKKLDV 582

BLAST of Lag0040066 vs. ExPASy TrEMBL
Match: A0A6J1HWP9 (protein NRT1/ PTR FAMILY 2.13-like OS=Cucurbita maxima OX=3661 GN=LOC111467544 PE=3 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 3.0e-281
Identity = 488/604 (80.79%), Postives = 531/604 (87.91%), Query Frame = 0

Query: 1   MEGQAQSKASSSPSKLHCFTKDADPKLCYSPEINKPKRKPGGWKSMPYILGNETFERLAS 60
           ME +  SKA+SSP+KLHC T D  PKL  SP+     RKPGGWKSMPYILGNETFERLAS
Sbjct: 1   MEVEGHSKAASSPTKLHCLTDDDSPKLSVSPQ-RSSGRKPGGWKSMPYILGNETFERLAS 60

Query: 61  MGLLANFMVYLRTVYHMDQVSATSLMSVWTGVTNFLPLVGAFLSDAYVGRYWTIAVASIF 120
           MGLLANFMVYL  VYHMDQVSAT+L++VWTGVTNFLPL+GAFLSDAYVGRYWTIAVAS+F
Sbjct: 61  MGLLANFMVYLIKVYHMDQVSATTLINVWTGVTNFLPLLGAFLSDAYVGRYWTIAVASVF 120

Query: 121 SLLGMASMTLTAWLPQLHPAECGGGEKCSGPTATHLGFLIMSLCLVSIGSAGIRPCSIPF 180
           SL GMASMTLTAWLP LHP  CGGG KC GPT+  +GFLIMSLC+VSIGS GIRPCSIPF
Sbjct: 121 SLFGMASMTLTAWLPALHPPACGGG-KCEGPTSFQMGFLIMSLCVVSIGSGGIRPCSIPF 180

Query: 181 GVDQFDPTTEKGRKGIASFYNWYYVSFTVVLVISLTIVVYIQDSVSWVLGYGIPTGLMVC 240
           GVDQFDPTT+ GRKGI SFYNWYY +F VVLV++LTIVVYIQDSVSWVLGYGIPT LM C
Sbjct: 181 GVDQFDPTTDAGRKGIVSFYNWYYTTFNVVLVVTLTIVVYIQDSVSWVLGYGIPTALMFC 240

Query: 241 SIVLFFMGTRVYVLMKPEGSIFTGLAQVAVAAYKKRKLQLPSFDAIDRAAFYDPPFDPAT 300
           SIVLFFMGT VYV +KPEGS+FTGL QVAVAAYKKRK++LPS +   R  FYDPP +P +
Sbjct: 241 SIVLFFMGTHVYVFIKPEGSVFTGLVQVAVAAYKKRKVELPSLETNHRTVFYDPPLEPGS 300

Query: 301 VVLSKLPLTNQFSFLNKAAMVVEEELNPDGTRINKWNLSTIQQVEEFKCLLRVAPIWVTG 360
           VVLSKLPLT+QF FLNKAA+V+E+ELNPDG R NKWNLSTIQQVEEFKCL+RVAPIWVTG
Sbjct: 301 VVLSKLPLTDQFRFLNKAAVVMEKELNPDGKRTNKWNLSTIQQVEEFKCLIRVAPIWVTG 360

Query: 361 ILSLTPIIQQSTFSISQALQMDRHMGPKFQIPAASIVVISFLTITFFIPLYDRFLVPALR 420
           ILSLTPIIQQ+TFSISQAL MDR +GP F IP ASI+VISF TIT F+P+YDRFLVP LR
Sbjct: 361 ILSLTPIIQQATFSISQALLMDRRIGPNFHIPPASIIVISFFTITLFVPIYDRFLVPFLR 420

Query: 421 NLTGHQNGITELQRMAIGIVFAVLSMVAAGLVEMARRNRANGPGGGSRMSVFWLTPQFVL 480
             T HQNGIT+LQRMA+GI+FAVLSMV AGL+EMARRNRAN   GGS MSVFWLTPQF L
Sbjct: 421 KFTNHQNGITQLQRMAVGIIFAVLSMVIAGLIEMARRNRAN--AGGSSMSVFWLTPQFFL 480

Query: 481 LGLCEAFNIIGQIEFFNKEFPEHMRTMGNALSSCSIALASYVSTAMVLIVHRTTGRGGAD 540
           +GLCEAFNIIGQIEFFNKEFPEHMRTMGNALSSCSIAL+SYVSTA++LIVH TTG G  D
Sbjct: 481 MGLCEAFNIIGQIEFFNKEFPEHMRTMGNALSSCSIALSSYVSTALILIVHGTTGGGSDD 540

Query: 541 GRPDWLADDLNRGRLDYFYYIVAATSFFNFFFFLYCAKNYRYKGKISDSE-DFELVSSEK 600
           GR DWLADDLN+GRLDYFYYIVAAT+FFNFF FLYCAKNYRYKGK S SE  FEL SSEK
Sbjct: 541 GRTDWLADDLNKGRLDYFYYIVAATAFFNFFLFLYCAKNYRYKGKESASELHFELASSEK 600

Query: 601 KLEV 604
           KLEV
Sbjct: 601 KLEV 600

BLAST of Lag0040066 vs. ExPASy TrEMBL
Match: A0A6J1FZM4 (protein NRT1/ PTR FAMILY 2.13-like OS=Cucurbita moschata OX=3662 GN=LOC111449345 PE=3 SV=1)

HSP 1 Score: 961.8 bits (2485), Expect = 1.3e-276
Identity = 482/604 (79.80%), Postives = 530/604 (87.75%), Query Frame = 0

Query: 1   MEGQAQSKASSSPSKLHCFTKDADPKLCYSPEINKPKRKPGGWKSMPYILGNETFERLAS 60
           ME +  SKA+SSP+KL C T D  PKL  S +     RKPGGWKSMPYILGNETFERLAS
Sbjct: 1   MEVEGHSKAASSPTKLRCLTDDDSPKLSISAQ-RSSGRKPGGWKSMPYILGNETFERLAS 60

Query: 61  MGLLANFMVYLRTVYHMDQVSATSLMSVWTGVTNFLPLVGAFLSDAYVGRYWTIAVASIF 120
           MGL+ANFMVYL  VYHMDQVSAT+L++VWTGVTNFLPL+GAFLSDAYVGRYWTIAVAS+F
Sbjct: 61  MGLIANFMVYLIKVYHMDQVSATTLINVWTGVTNFLPLLGAFLSDAYVGRYWTIAVASVF 120

Query: 121 SLLGMASMTLTAWLPQLHPAECGGGEKCSGPTATHLGFLIMSLCLVSIGSAGIRPCSIPF 180
           SL GMASMTLTAWLP LHP  CGGG KC GPT   +GFLIMSLC+VSIGS GIRPCSIPF
Sbjct: 121 SLFGMASMTLTAWLPALHPPACGGG-KCEGPTPFQMGFLIMSLCVVSIGSGGIRPCSIPF 180

Query: 181 GVDQFDPTTEKGRKGIASFYNWYYVSFTVVLVISLTIVVYIQDSVSWVLGYGIPTGLMVC 240
           GVDQFDPTT+ GRKGIASFYNWYY +F VVLV++LTIVVYIQDSVSWVLGYGIPT LM C
Sbjct: 181 GVDQFDPTTDAGRKGIASFYNWYYATFNVVLVVTLTIVVYIQDSVSWVLGYGIPTALMFC 240

Query: 241 SIVLFFMGTRVYVLMKPEGSIFTGLAQVAVAAYKKRKLQLPSFDAIDRAAFYDPPFDPAT 300
           SIVLFFMGT VYV +KPEGS+FTGL QVAVAAY+KRK++LPS +   R AFYDPP +P +
Sbjct: 241 SIVLFFMGTHVYVFIKPEGSVFTGLVQVAVAAYRKRKVELPSLETNHRTAFYDPPLEPGS 300

Query: 301 VVLSKLPLTNQFSFLNKAAMVVEEELNPDGTRINKWNLSTIQQVEEFKCLLRVAPIWVTG 360
           VVLSKLPLT+QF FLNKAA+V+E+ELNPDG R NKWNLSTIQQVEEFKCL+RVAPIWVTG
Sbjct: 301 VVLSKLPLTDQFRFLNKAAVVMEKELNPDGKRTNKWNLSTIQQVEEFKCLIRVAPIWVTG 360

Query: 361 ILSLTPIIQQSTFSISQALQMDRHMGPKFQIPAASIVVISFLTITFFIPLYDRFLVPALR 420
           ILSLTP++QQ+TFSISQAL MDR +GP F IP ASI+VISFLTIT F+P+YDRFLVP LR
Sbjct: 361 ILSLTPMLQQATFSISQALLMDRRIGPNFHIPPASIIVISFLTITLFVPIYDRFLVPFLR 420

Query: 421 NLTGHQNGITELQRMAIGIVFAVLSMVAAGLVEMARRNRANGPGGGSRMSVFWLTPQFVL 480
             T HQNGIT+LQRMA+GI+FAVLSMV AGL+EMARRN+AN   GG  MSVFWLTPQF L
Sbjct: 421 KFTHHQNGITQLQRMAVGILFAVLSMVIAGLIEMARRNQAN--AGGGSMSVFWLTPQFFL 480

Query: 481 LGLCEAFNIIGQIEFFNKEFPEHMRTMGNALSSCSIALASYVSTAMVLIVHRTTGRGGAD 540
           +GLCEAFNIIGQIEFFNKEFPEHMRTMGNALSSCSIAL+SYVSTA+VL VH TT   G D
Sbjct: 481 MGLCEAFNIIGQIEFFNKEFPEHMRTMGNALSSCSIALSSYVSTALVLFVHGTT---GGD 540

Query: 541 GRPDWLADDLNRGRLDYFYYIVAATSFFNFFFFLYCAKNYRYKGKISDSE-DFELVSSEK 600
           GR DWLADDLN+GRLDYFYYIVAAT+FFNFF FLYCAKNYRYKGK S+SE DFEL SS+K
Sbjct: 541 GRTDWLADDLNKGRLDYFYYIVAATAFFNFFLFLYCAKNYRYKGKESNSELDFELASSDK 597

Query: 601 KLEV 604
           KLEV
Sbjct: 601 KLEV 597

BLAST of Lag0040066 vs. ExPASy TrEMBL
Match: A0A1S3BEQ4 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 2.13-like OS=Cucumis melo OX=3656 GN=LOC103489074 PE=3 SV=1)

HSP 1 Score: 961.8 bits (2485), Expect = 1.3e-276
Identity = 492/608 (80.92%), Postives = 531/608 (87.34%), Query Frame = 0

Query: 1   MEGQAQSKASSSPSKLHCFTKDA---DPKLCYSPEINKPKRKPGGWKSMPYILGNETFER 60
           ME +++ K SS+    + F KDA   +PKL  S   NK KR+PGGWKSMPYILGNETFER
Sbjct: 1   MESESEKKGSSNSHSPNNF-KDANNNNPKLSIS---NKSKREPGGWKSMPYILGNETFER 60

Query: 61  LASMGLLANFMVYLRTVYHMDQVSATSLMSVWTGVTNFLPLVGAFLSDAYVGRYWTIAVA 120
           LASMGLLANFMVYL+TVYHMDQVSATSLM +WTGVT+FLPL+GAFLSDAY+GRYWTIA+A
Sbjct: 61  LASMGLLANFMVYLKTVYHMDQVSATSLMGLWTGVTSFLPLLGAFLSDAYIGRYWTIAIA 120

Query: 121 SIFSLLGMASMTLTAWLPQLHPAECGGGEKCSGPTATHLGFLIMSLCLVSIGSAGIRPCS 180
           SIFS LGMASMTLTAWLPQLHP  CGG  KC  PTA+ LGFLIMSLCLVSIGS GIRPCS
Sbjct: 121 SIFSFLGMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCS 180

Query: 181 IPFGVDQFDPTTEKGRKGIASFYNWYYVSFTVVLVISLTIVVYIQDSVSWVLGYGIPTGL 240
           IPFGVDQFDPTTEKGRKGIASFYNWYY +F VVLV +LTIVVYIQDSVSWVLGYGIPTGL
Sbjct: 181 IPFGVDQFDPTTEKGRKGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGL 240

Query: 241 MVCSIVLFFMGTRVYVLMKPEGSIFTGLAQVAVAAYKKRKLQLPSFDAIDRAAFYDPPFD 300
           M CSI+LFFMGT VYV +KPEGSIFTGLAQVAVAAYKKR L+LP     DR  FYDPP D
Sbjct: 241 MFCSIILFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPD----DRKGFYDPPLD 300

Query: 301 PATVVLSKLPLTNQFSFLNKAAMVVEEELNPDGTRINKWNLSTIQQVEEFKCLLRVAPIW 360
           PA+VVLSKLPLTNQFSFLNKAA+V E E+ PDGTR+NKWNLS+IQQVEE KCL RV PIW
Sbjct: 301 PASVVLSKLPLTNQFSFLNKAAIVTENEIKPDGTRLNKWNLSSIQQVEELKCLFRVFPIW 360

Query: 361 VTGILSLTPIIQQSTFSISQALQMDRHMGPKFQIPAASIVVISFLTITFFIPLYDRFLVP 420
           VTGILSLTPIIQQSTFSISQALQM+RHMG  FQIP ASI+VISFLTITFFIPLYDRFLVP
Sbjct: 361 VTGILSLTPIIQQSTFSISQALQMNRHMGSNFQIPPASIIVISFLTITFFIPLYDRFLVP 420

Query: 421 ALRNLTGHQNGITELQRMAIGIVFAVLSMVAAGLVEMARRNRANGPGGGSRMSVFWLTPQ 480
            LR  TGH NGITELQRMAIGI+FAVLSMV AGLVEM RRN+AN    GS MSVFWLTPQ
Sbjct: 421 TLRKFTGHPNGITELQRMAIGIIFAVLSMVVAGLVEMQRRNQAN--NDGSIMSVFWLTPQ 480

Query: 481 FVLLGLCEAFNIIGQIEFFNKEFPEHMRTMGNALSSCSIALASYVSTAMVLIVHRTTGRG 540
           F L+GLCEAFNIIGQIEFFNKEFPEHMRTMGNA SSCSIAL+SY++TAMVLIVHRTTGRG
Sbjct: 481 FFLMGLCEAFNIIGQIEFFNKEFPEHMRTMGNAFSSCSIALSSYINTAMVLIVHRTTGRG 540

Query: 541 GADGRPDWLADDLNRGRLDYFYYIVAATSFFNFFFFLYCAKNYRYKGKISDSE-DFEL-V 600
           GA G+PDWL DDLN+GRLDYFYYIVAAT+ F  F FLYCAKNYRYKG +SD E D +L V
Sbjct: 541 GAHGKPDWLIDDLNKGRLDYFYYIVAATAXFVIFLFLYCAKNYRYKGHVSDDEQDLQLVV 598

Query: 601 SSEKKLEV 604
           SS+K L+V
Sbjct: 601 SSDKLLDV 598

BLAST of Lag0040066 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 657.1 bits (1694), Expect = 1.3e-188
Identity = 336/613 (54.81%), Postives = 447/613 (72.92%), Query Frame = 0

Query: 1   MEGQAQSKASSSPSKLHCFTKDADP-KLCYSP----EINKPKRKPGGWKSMPYILGNETF 60
           + G++ S + SSPS+L       DP K   SP    +  K ++KPGGW+++ +ILGNET 
Sbjct: 12  LPGRSGSFSKSSPSELDV----VDPYKRISSPGSILDAEKVEKKPGGWRAVSFILGNETL 71

Query: 61  ERLASMGLLANFMVYLRTVYHMDQVSATSLMSVWTGVTNFLPLVGAFLSDAYVGRYWTIA 120
           ERL S+GLLANFMVYL  V+H++QV A +++++W+G TN  PLVGA++SD YVGR+ TIA
Sbjct: 72  ERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVGAYISDTYVGRFKTIA 131

Query: 121 VASIFSLLGMASMTLTAWLPQLHPAECGGGE--KCSGPTATHLGFLIMSLCLVSIGSAGI 180
            AS  +LLG+ ++TLTA  PQLHPA C   +   C GP    +G L++ LC +S+GS GI
Sbjct: 132 FASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVLLLGLCFLSVGSGGI 191

Query: 181 RPCSIPFGVDQFDPTTEKGRKGIASFYNWYYVSFTVVLVISLTIVVYIQDSVSWVLGYGI 240
           RPCSIPFGVDQFD  TE+G KG+ASF+NWYY++FTVVL+I+ T+VVYIQD VSW++G+ I
Sbjct: 192 RPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVVVYIQDQVSWIIGFSI 251

Query: 241 PTGLMVCSIVLFFMGTRVYVLMKPEGSIFTGLAQVAVAAYKKRKLQLPSFDAIDRAAFYD 300
           PTGLM  ++V+FF G + YV +KPEGSIF+G+AQV VAA KKRKL+LP+ D      +YD
Sbjct: 252 PTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLKLPAEDD-GTVTYYD 311

Query: 301 PPFDPATVVLSKLPLTNQFSFLNKAAMVVEEELNPDGTRINKWNLSTIQQVEEFKCLLRV 360
           P    +  VLSKL  +NQF  L+KAA+V+E +L P+G   +KW L ++Q+VEE KCL+R+
Sbjct: 312 PAIKSS--VLSKLHRSNQFRCLDKAAVVIEGDLTPEGPPADKWRLCSVQEVEEVKCLIRI 371

Query: 361 APIWVTGILSLTPIIQQSTFSISQALQMDRHMGPKFQIPAASIVVISFLTITFFIPLYDR 420
            PIW  GI+SL  +  Q TF++SQAL+MDR++GPKF+IPA S+ VIS LTI  F+P YDR
Sbjct: 372 VPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVISLLTIGIFLPFYDR 431

Query: 421 FLVPALRNLTGHQNGITELQRMAIGIVFAVLSMVAAGLVEMARRNR---ANGPGGGSRMS 480
             VP +R +TGH++GIT LQR+  GIVFA+ SM+ AG+VE  RR R   A  P G + MS
Sbjct: 432 VFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIRSINAGDPTGMTPMS 491

Query: 481 VFWLTPQFVLLGLCEAFNIIGQIEFFNKEFPEHMRTMGNALSSCSIALASYVSTAMVLIV 540
           VFWL+PQ +L+GLCEAFNIIGQIEFFN +FPEHMR++ N+L S S A +SY+S+ +V +V
Sbjct: 492 VFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSFAGSSYLSSFLVTVV 551

Query: 541 HRTTGRGGADGRPDWLADDLNRGRLDYFYYIVAATSFFNFFFFLYCAKNYRYKGKISDSE 600
           H+ +  GG D RPDWL  +LN G+LDYFYY++A     N  +F YCA+ YRYK  +   E
Sbjct: 552 HKFS--GGHD-RPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFWYCARGYRYKVGL-PIE 611

Query: 601 DFELVSSEKKLEV 604
           DFE   S   +E+
Sbjct: 612 DFEEDKSSDDVEM 613

BLAST of Lag0040066 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 599.4 bits (1544), Expect = 3.3e-171
Identity = 303/568 (53.35%), Postives = 407/568 (71.65%), Query Frame = 0

Query: 36  PKRKPGGWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVSATSLMSVWTGVTNF 95
           P++K GGW+++ +ILGNET E+L S+G+ ANFM+YLR V+HM+ V A ++  +W G+TNF
Sbjct: 11  PEKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNF 70

Query: 96  LPLVGAFLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAECGG--GEKCSGPTA 155
            PL+GA +SDAY+GR+ TIA AS+FS+LG+ ++TLTA LPQLHP  C     ++C  P  
Sbjct: 71  APLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNK 130

Query: 156 THLGFLIMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYVSFTVVLVI 215
             LG L + L  +SIGS GIRPCSIPFGVDQFD  TE+G KG+ASF+NWYY++ T+VL+ 
Sbjct: 131 LQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIF 190

Query: 216 SLTIVVYIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSIFTGLAQVAVAAY 275
           S T+VVY+Q +VSWV+G+ IPT LM C++VLFF+G R YV +KPEGS+F+G+A+V VAA 
Sbjct: 191 SHTVVVYLQ-TVSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAAR 250

Query: 276 KKRKLQLPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMVVEEELNPDGTRI 335
           KKR L++   D      +Y+PP  P   VLSKLPLT+QF FL+KAA++++ +L  +G   
Sbjct: 251 KKRDLKISLVDD-GTEEYYEPPVKPG--VLSKLPLTDQFKFLDKAAVILDGDLTSEGVPA 310

Query: 336 NKWNLSTIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQMDRHMGPKFQIPA 395
           NKW L +IQ+VEE KCL+RV P+W  GI+S+  +  Q+TF + QA +MDRHMGP F+IPA
Sbjct: 311 NKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPA 370

Query: 396 ASIVVISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVFAVLSMVAAGLVE 455
           ASI VIS++TI  ++P+Y+  LVP L  +   +  +T LQRM IGIVFA+LSM  AG VE
Sbjct: 371 ASITVISYITIGIWVPIYEHLLVPFLWRMRKFR--VTLLQRMGIGIVFAILSMFTAGFVE 430

Query: 456 MARRNRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEFPEHMRTMGNALSS 515
             RR RA      ++MSVFWL    +L+GLCE+FN IG IEFFN +FPEHMR++ N+L  
Sbjct: 431 GVRRTRATEM---TQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFP 490

Query: 516 CSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYYIVAATSFFNFFFF 575
            S A A+Y+S+ +V  VH+ +   G    PDWL  DL+RG+LDYFYY++A     N  +F
Sbjct: 491 LSFAAANYLSSLLVTTVHKVS---GTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVNLVYF 550

Query: 576 LYCAKNYRYKGKISDSEDFELVSSEKKL 602
            YCA  Y+YK   S  EDF   + EK L
Sbjct: 551 WYCAHRYQYKAG-SQIEDF---NEEKSL 562

BLAST of Lag0040066 vs. TAIR 10
Match: AT1G69860.1 (Major facilitator superfamily protein )

HSP 1 Score: 502.3 bits (1292), Expect = 5.5e-142
Identity = 256/558 (45.88%), Postives = 376/558 (67.38%), Query Frame = 0

Query: 30  SPEINKPKRKPGGWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVSATSLMSVW 89
           S ++   +RKP GWK+MPYI+GNET ERLA+ GL+ANFMVY+   YHMDQV A +L++ W
Sbjct: 9   SSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINTW 68

Query: 90  TGVTNFLPLVGAFLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAECGGGE--- 149
           + +TNF P++GAF+SD+Y G++ TI   SI  LLGM  +T T+ +P L P  C   +   
Sbjct: 69  SALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPCTADQITG 128

Query: 150 KCSGPTATHLGFLIMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYVS 209
           +C   + + L  L+  L L+S+G+ GIR CS+PF +DQFD +TE+GR+G  SF++WYY +
Sbjct: 129 QCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYYTT 188

Query: 210 FTVVLVISLTIVVYIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSIFTGLA 269
            T+V ++S+T+V+Y+Q+++SW +G+ IPT L   +++L F+GTR YV +KPEGS+F+G+ 
Sbjct: 189 HTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSGVF 248

Query: 270 QVAVAAYKKRKLQLPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMVVEEEL 329
           +V VAAYKKRK +  S   ID    Y  P     +  +KL LT+QF FLNKA +V    +
Sbjct: 249 KVLVAAYKKRKARFTS--GID----YHQPLLETDLQSNKLVLTDQFRFLNKAVIV----M 308

Query: 330 NPDGTRINKWNLSTIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQMD-RHM 389
           N D     +W   T++Q+E+ K ++ + PI+ + I+    + QQ TF++SQAL+MD +  
Sbjct: 309 NNDEAGNEEWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFP 368

Query: 390 GPKFQIPAASIVVISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVFAVLS 449
           G  + IP ASI VIS L I  ++P Y+  LV  + N+T    GI+ LQ++ IG +F++ +
Sbjct: 369 GTSYLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSIST 428

Query: 450 MVAAGLVEMARRNRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEFPEHMR 509
           M+ +G+VE  RR+ +     G +MSVFWLTPQ VL+G  + F I+G  EFFNK+ P +MR
Sbjct: 429 MLISGIVERKRRDLSL---NGVKMSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMR 488

Query: 510 TMGNALSSCSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYYIVAAT 569
           ++GN+L    ++LASY+S+AMV IVH  T RGG      WL DD+++ +LD FYY +AA 
Sbjct: 489 SIGNSLLYLGLSLASYLSSAMVSIVHSVTARGG----QSWLTDDIDKSKLDCFYYFIAAL 548

Query: 570 SFFNFFFFLYCAKNYRYK 584
           S  NF FF +CA+ YRY+
Sbjct: 549 STLNFIFFFWCARRYRYR 549

BLAST of Lag0040066 vs. TAIR 10
Match: AT1G18880.1 (Major facilitator superfamily protein )

HSP 1 Score: 469.9 bits (1208), Expect = 3.0e-132
Identity = 246/570 (43.16%), Postives = 364/570 (63.86%), Query Frame = 0

Query: 42  GWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVSATSLMSVWTGVTNFLPLVGA 101
           GWK MP+I+GNETFE+L  +G  +N ++YL TV++M  ++A  +++++ G +NF  +V A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 102 FLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAECGG--GEKCSGPTATHLGFL 161
           FL D+Y GRY T++ A I   LG  +M LTA +  LHPA+C    G  C+GP+   + FL
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNGPSIGQIMFL 141

Query: 162 IMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYVSFTVVLVISLTIVV 221
             ++ L+ IG+ GIRPC++PFG DQFDP T++G++GI SF+NWY+ +FT   ++SLT++V
Sbjct: 142 AGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIESFFNWYFFTFTFAQMVSLTLIV 201

Query: 222 YIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSIFTGLAQVAVAAYKKRKLQ 281
           Y+Q +VSW +G  IP  LM+   ++FF G+++YV +K  GS    + +V V A KKR+L+
Sbjct: 202 YVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRLK 261

Query: 282 LPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMVVEEE-LNPDGTRINKWNL 341
                 +     Y+  +  +    SKL  T QF FL+K+A+  +++ LN DG+ ++ W L
Sbjct: 262 -----PVGPNELYN--YIASDFKNSKLGHTEQFRFLDKSAIQTQDDKLNKDGSPVDAWKL 321

Query: 342 STIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQMDRHMGP-KFQIPAASIV 401
            ++QQVEE KC++RV P+W++  L     IQQ+T++I Q+LQ DR +GP  FQIPA S  
Sbjct: 322 CSMQQVEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYT 381

Query: 402 VISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVFAVLSMVAAGLVEMARR 461
           V   L +T FIP+YDR LVP LR  TG   GIT+LQR+  G+   + SM+ + +VE  RR
Sbjct: 382 VFLMLGMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRR 441

Query: 462 ---------NRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEFPEHMRTMG 521
                      A   G  S MS  WL PQ VL+G+ +A   +GQ+EF+ K+FPE+MR+  
Sbjct: 442 KVALTKPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFA 501

Query: 522 NALSSCSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYYIVAATSFF 581
            +L  C I LASY+ST ++  VH TT  G + G   WL +DLN+GRL+YFY++VA     
Sbjct: 502 GSLYYCGIGLASYLSTFLLSAVHDTT-EGFSGG--SWLPEDLNKGRLEYFYFLVAGMMTL 561

Query: 582 NFFFFLYCAKNYRYKGKISDSEDFELVSSE 599
           N  +FL  +  YRYK  ++  +D +  S+E
Sbjct: 562 NLAYFLLVSHWYRYKDVVAKDKDMDKTSAE 581

BLAST of Lag0040066 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 464.5 bits (1194), Expect = 1.3e-130
Identity = 250/573 (43.63%), Postives = 352/573 (61.43%), Query Frame = 0

Query: 34  NKPKRKPGGWKSMPYILGNETFERLASMGLLANFMVYLRTVYHMDQVSATSLMSVWTGVT 93
           N+ K    GWK MP+I+GNETFE+L  +G L+N +VYL  V+++  ++A ++++ ++G  
Sbjct: 38  NQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTI 97

Query: 94  NFLPLVGAFLSDAYVGRYWTIAVASIFSLLGMASMTLTAWLPQLHPAECG--GGEKCSGP 153
           NF   V AFL D Y GRY T++VA I   LG   + LTA +PQLHPA CG      C+GP
Sbjct: 98  NFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGP 157

Query: 154 TATHLGFLIMSLCLVSIGSAGIRPCSIPFGVDQFDPTTEKGRKGIASFYNWYYVSFTVVL 213
           +   + FL+M L  + +G+ GIRPC++ FG DQF+P +E G++GI SF+NWY+ +FT   
Sbjct: 158 SGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQ 217

Query: 214 VISLTIVVYIQDSVSWVLGYGIPTGLMVCSIVLFFMGTRVYVLMKPEGSIFTGLAQVAVA 273
           ++SLT+VVY+Q +VSW +G  IP  LM  + ++FF G ++YV +K  GS   G+AQV   
Sbjct: 218 ILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAV 277

Query: 274 AYKKRKLQLPSFDAIDRAAFYDPPFDPATVVLSKLPLTNQFSFLNKAAMVV-EEELNPDG 333
           A KKR L+      ++   +Y P +       SKL  T+QF FL+KAA++  E++L PDG
Sbjct: 278 AIKKRGLKPAKQPWLNLYNYYPPKY-----ANSKLKYTDQFRFLDKAAILTPEDKLQPDG 337

Query: 334 TRINKWNLSTIQQVEEFKCLLRVAPIWVTGILSLTPIIQQSTFSISQALQMDRHMGP-KF 393
              + W L T+QQVEE KC++RV PIW    +    I QQ T+ + QALQ DR +G   F
Sbjct: 338 KPADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGF 397

Query: 394 QIPAASIVVISFLTITFFIPLYDRFLVPALRNLTGHQNGITELQRMAIGIVFAVLSMVAA 453
            IPAA+ VV     +T FI +YDR LVP +R +TG   GIT LQR+  GI FA  S+V A
Sbjct: 398 VIPAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVA 457

Query: 454 GLVEMARR---------NRANGPGGGSRMSVFWLTPQFVLLGLCEAFNIIGQIEFFNKEF 513
           G VE  RR           A   G  S MS  WL PQ  L G+ EAF  IGQ+EF+ K+F
Sbjct: 458 GFVEERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQF 517

Query: 514 PEHMRTMGNALSSCSIALASYVSTAMVLIVHRTTGRGGADGRPDWLADDLNRGRLDYFYY 573
           PE+MR+   ++      ++SY+ + ++  VHRTT         +WLA+DLN+GRLD FY+
Sbjct: 518 PENMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGG---NWLAEDLNKGRLDLFYF 577

Query: 574 IVAATSFFNFFFFLYCAKNYRYKGKISDSEDFE 594
           ++A     NF +FL  ++ YRYKG   +   +E
Sbjct: 578 MIAGILAVNFAYFLVMSRWYRYKGSDDEVTTYE 602