Lag0040021 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0040021
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	MLP-like protein 423
Location	chr13: 1616242 .. 1616784 (-)
RNA-Seq Expression	Lag0040021
Synteny	Lag0040021

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTGATGGAACGCTTAGCGTAGAGGTTGACGTGAAGTCCATCGCACGCAAGTTCTGGACTTGCATCAGGGACTCAACCACCATCTTCCCCAAGGCCTTCCCCCATGACTACAAAAGCATCGAAGTTCTTGAAGGCGATGGGAAAGCTGTCGCTTCTGTTCGCCTCATTACGTACTCCGAGGGTAAAACTCTAGACATAACTCTGGCTACTTTCTCATACATGACATCACTCATTTTGTTGTGAATTTCTGTATCAGGTTCTTCGCTCGTTAAAGAGTCGAAAGAGAGGATCGAGGCCGTGGACGAGGAAAAGATGACGGTGTCTTACAGCGTGATAGAAGGGGATCTTCTCAAGTACTACAAGAGTTTCAAGGGGCACATTGTTGTGAGTCCTAAAGAAGATGGAAGTGGAAGTTCAGTGAAATGGAGCTGTGAGTTTGAGAAGGCGAGTGAGGAGGTTCCTGATCCTCATGTCATTAAGGACTTTGTTGTGAAGAATTTCCTGGAAGTCGACGCCTATGCTCTGCTGCAGGCATAG

mRNA sequence

ATGGCTTCTGATGGAACGCTTAGCGTAGAGGTTGACGTGAAGTCCATCGCACGCAAGTTCTGGACTTGCATCAGGGACTCAACCACCATCTTCCCCAAGGCCTTCCCCCATGACTACAAAAGCATCGAAGTTCTTGAAGGCGATGGGAAAGCTGTCGCTTCTGTTCGCCTCATTACGTACTCCGAGGGTTCTTCGCTCGTTAAAGAGTCGAAAGAGAGGATCGAGGCCGTGGACGAGGAAAAGATGACGGTGTCTTACAGCGTGATAGAAGGGGATCTTCTCAAGTACTACAAGAGTTTCAAGGGGCACATTGTTGTGAGTCCTAAAGAAGATGGAAGTGGAAGTTCAGTGAAATGGAGCTGTGAGTTTGAGAAGGCGAGTGAGGAGGTTCCTGATCCTCATGTCATTAAGGACTTTGTTGTGAAGAATTTCCTGGAAGTCGACGCCTATGCTCTGCTGCAGGCATAG

Coding sequence (CDS)

ATGGCTTCTGATGGAACGCTTAGCGTAGAGGTTGACGTGAAGTCCATCGCACGCAAGTTCTGGACTTGCATCAGGGACTCAACCACCATCTTCCCCAAGGCCTTCCCCCATGACTACAAAAGCATCGAAGTTCTTGAAGGCGATGGGAAAGCTGTCGCTTCTGTTCGCCTCATTACGTACTCCGAGGGTTCTTCGCTCGTTAAAGAGTCGAAAGAGAGGATCGAGGCCGTGGACGAGGAAAAGATGACGGTGTCTTACAGCGTGATAGAAGGGGATCTTCTCAAGTACTACAAGAGTTTCAAGGGGCACATTGTTGTGAGTCCTAAAGAAGATGGAAGTGGAAGTTCAGTGAAATGGAGCTGTGAGTTTGAGAAGGCGAGTGAGGAGGTTCCTGATCCTCATGTCATTAAGGACTTTGTTGTGAAGAATTTCCTGGAAGTCGACGCCTATGCTCTGCTGCAGGCATAG

Protein sequence

MASDGTLSVEVDVKSIARKFWTCIRDSTTIFPKAFPHDYKSIEVLEGDGKAVASVRLITYSEGSSLVKESKERIEAVDEEKMTVSYSVIEGDLLKYYKSFKGHIVVSPKEDGSGSSVKWSCEFEKASEEVPDPHVIKDFVVKNFLEVDAYALLQA

Homology

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BLAST of Lag0040021 vs. NCBI nr
Match: XP_022942327.1 (MLP-like protein 423 [Cucurbita moschata] >KAG6600129.1 MLP-like protein 423, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 274.6 bits (701), Expect = 5.1e-70
Identity = 138/154 (89.61%), Postives = 142/154 (92.21%), Query Frame = 0


Query: 1   MASDGTLSVEVDVKSIARKFWTCIRDSTTIFPKAFPHDYKSIEVLEGDGKAVASVRLITY 60
           MASDGTL+VEVDVKSIA KFW  +RDST IFPKAFPHDYKSIEVLEGDGKAV SVRLITY
Sbjct: 1   MASDGTLNVEVDVKSIAPKFWNSMRDSTIIFPKAFPHDYKSIEVLEGDGKAVGSVRLITY 60

Query: 61  SEGSSLVKESKERIEAVDEEKMTVSYSVIEGDLLKYYKSFKGHIVVSPKEDGSGSSVKWS 120
           SEGSSLVK+SKERIEAVDEE MTVSYSVIEGDLLKYYKSFKGHI V PKEDGSGS VKWS
Sbjct: 61  SEGSSLVKDSKERIEAVDEEAMTVSYSVIEGDLLKYYKSFKGHIGVIPKEDGSGSKVKWS 120

Query: 121 CEFEKASEEVPDPHVIKDFVVKNFLEVDAYALLQ 155
           CEFEKASEEVPDPHVIKDFVVKNFLE+D Y L Q
Sbjct: 121 CEFEKASEEVPDPHVIKDFVVKNFLELDDYVLQQ 154

[Close]

BLAST of Lag0040021 vs. NCBI nr
Match: XP_023553168.1 (MLP-like protein 423 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 274.2 bits (700), Expect = 6.7e-70
Identity = 137/154 (88.96%), Postives = 142/154 (92.21%), Query Frame = 0


Query: 1   MASDGTLSVEVDVKSIARKFWTCIRDSTTIFPKAFPHDYKSIEVLEGDGKAVASVRLITY 60
           MASDGTL+VEVDVKS+A KFW  +RDST IFPKAFPHDYKSIEVLEGDGKAV SVRLITY
Sbjct: 1   MASDGTLNVEVDVKSVAPKFWNSMRDSTIIFPKAFPHDYKSIEVLEGDGKAVGSVRLITY 60

Query: 61  SEGSSLVKESKERIEAVDEEKMTVSYSVIEGDLLKYYKSFKGHIVVSPKEDGSGSSVKWS 120
           SEGSSLVK+SKERIEAVDEE MTVSYSVIEGDLLKYYKSFKGHI V PKEDGSGS VKWS
Sbjct: 61  SEGSSLVKDSKERIEAVDEEAMTVSYSVIEGDLLKYYKSFKGHIGVIPKEDGSGSKVKWS 120

Query: 121 CEFEKASEEVPDPHVIKDFVVKNFLEVDAYALLQ 155
           CEFEKASEEVPDPHVIKDFVVKNFLE+D Y L Q
Sbjct: 121 CEFEKASEEVPDPHVIKDFVVKNFLELDDYVLQQ 154

[Close]

BLAST of Lag0040021 vs. NCBI nr
Match: XP_022985665.1 (MLP-like protein 423 [Cucurbita maxima])

HSP 1 Score: 272.7 bits (696), Expect = 1.9e-69
Identity = 137/154 (88.96%), Postives = 141/154 (91.56%), Query Frame = 0


Query: 1   MASDGTLSVEVDVKSIARKFWTCIRDSTTIFPKAFPHDYKSIEVLEGDGKAVASVRLITY 60
           MASDGTL+VEVDVKSIA KFW  +RDS  IFPKAFPHDYKSIEVLEGDGKAV SVRLITY
Sbjct: 1   MASDGTLNVEVDVKSIAPKFWNSMRDSAIIFPKAFPHDYKSIEVLEGDGKAVGSVRLITY 60

Query: 61  SEGSSLVKESKERIEAVDEEKMTVSYSVIEGDLLKYYKSFKGHIVVSPKEDGSGSSVKWS 120
           SEGSSLVK+SKERIEAVDEE MTVSYSVIEGDLLKYYKSFKGHI V PKEDGSGS VKWS
Sbjct: 61  SEGSSLVKDSKERIEAVDEEAMTVSYSVIEGDLLKYYKSFKGHIGVIPKEDGSGSKVKWS 120

Query: 121 CEFEKASEEVPDPHVIKDFVVKNFLEVDAYALLQ 155
           CEFEKASEEVPDPHVIKDFVVKNFLE+D Y L Q
Sbjct: 121 CEFEKASEEVPDPHVIKDFVVKNFLELDDYVLQQ 154

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BLAST of Lag0040021 vs. NCBI nr
Match: XP_008446239.1 (PREDICTED: MLP-like protein 423 [Cucumis melo] >KAA0034333.1 MLP-like protein 423 [Cucumis melo var. makuwa] >TYK15586.1 MLP-like protein 423 [Cucumis melo var. makuwa])

HSP 1 Score: 267.3 bits (682), Expect = 8.2e-68
Identity = 132/155 (85.16%), Postives = 140/155 (90.32%), Query Frame = 0


Query: 1   MASDGTLSVEVDVKSIARKFWTCIRDSTTIFPKAFPHDYKSIEVLEGDGKAVASVRLITY 60
           MASDGTLSVEVDVKS+A KFW  +RDST IFPKAFPHDYKSIEVLEGDGKAV S+RLITY
Sbjct: 1   MASDGTLSVEVDVKSVADKFWKNMRDSTIIFPKAFPHDYKSIEVLEGDGKAVGSIRLITY 60

Query: 61  SEGSSLVKESKERIEAVDEEKMTVSYSVIEGDLLKYYKSFKGHIVVSPKEDGSGSSVKWS 120
           SEGS +VKESKERIEA DEEK TVSYSVI GDLLKYYKSFKGHIVV PKEDG+GSSVKWS
Sbjct: 61  SEGSPIVKESKERIEAADEEKKTVSYSVIGGDLLKYYKSFKGHIVVIPKEDGNGSSVKWS 120

Query: 121 CEFEKASEEVPDPHVIKDFVVKNFLEVDAYALLQA 156
           CEFEKASEE+PDPH IKDFVVKNF+E+D Y   QA
Sbjct: 121 CEFEKASEEIPDPHAIKDFVVKNFMELDDYCHQQA 155

[Close]

BLAST of Lag0040021 vs. NCBI nr
Match: XP_038892372.1 (MLP-like protein 423 [Benincasa hispida])

HSP 1 Score: 265.0 bits (676), Expect = 4.1e-67
Identity = 132/155 (85.16%), Postives = 140/155 (90.32%), Query Frame = 0


Query: 1   MASDGTLSVEVDVKSIARKFWTCIRDSTTIFPKAFPHDYKSIEVLEGDGKAVASVRLITY 60
           MASDG LSVEV+VKS+A KFW  +RDST IFPKAFPHDYKSI+VLEGDGKAV SVRLITY
Sbjct: 1   MASDGMLSVEVEVKSVADKFWNSMRDSTIIFPKAFPHDYKSIDVLEGDGKAVGSVRLITY 60

Query: 61  SEGSSLVKESKERIEAVDEEKMTVSYSVIEGDLLKYYKSFKGHIVVSPKEDGSGSSVKWS 120
           SEGS +VKESKERIEAVDEEK +VSYSVIEGDLLKYYKSFKGHI VSPKED SGS VKWS
Sbjct: 61  SEGSPIVKESKERIEAVDEEKRSVSYSVIEGDLLKYYKSFKGHITVSPKEDESGSLVKWS 120

Query: 121 CEFEKASEEVPDPHVIKDFVVKNFLEVDAYALLQA 156
           CEFEKASEEVPDPH+IKDFVVKNF E+D Y L QA
Sbjct: 121 CEFEKASEEVPDPHLIKDFVVKNFSELDDYVLKQA 155

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BLAST of Lag0040021 vs. ExPASy Swiss-Prot
Match: Q93VR4 (MLP-like protein 423 OS=Arabidopsis thaliana OX=3702 GN=MLP423 PE=2 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 8.3e-47
Identity = 94/154 (61.04%), Postives = 113/154 (73.38%), Query Frame = 0


Query: 1   MASDGTLSVEVDVKSIARKFWTCIRDSTTIFPKAFPHDYKSIEVLEGDGKAVASVRLITY 60
           M   G L VEV+VKS A KFW  + D   +FPKAFP+DYK+I+VL GDG A  S+RLITY
Sbjct: 1   MGLSGVLHVEVEVKSPAEKFWVALGDGINLFPKAFPNDYKTIQVLAGDGNAPGSIRLITY 60

Query: 61  SEGSSLVKESKERIEAVDEEKMTVSYSVIEGDLLKYYKSFKGHIVVSPKEDGSGSSVKWS 120
            EGS LVK S ERIEAVD E  ++SYS+I G++L+YYK+FKG I V PK+   GS +KWS
Sbjct: 61  GEGSPLVKISAERIEAVDLENKSMSYSIIGGEMLEYYKTFKGTITVIPKD--GGSLLKWS 120

Query: 121 CEFEKASEEVPDPHVIKDFVVKNFLEVDAYALLQ 155
            EFEK + E+ DPHVIKDF VKNF E+D Y L Q
Sbjct: 121 GEFEKTAHEIDDPHVIKDFAVKNFKEIDEYLLKQ 152

[Close]

BLAST of Lag0040021 vs. ExPASy Swiss-Prot
Match: Q9SSK7 (MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 7.0e-22
Identity = 62/149 (41.61%), Postives = 83/149 (55.70%), Query Frame = 0


Query: 6   TLSVEVDVKSIARKFWTCIRDSTTIFPKAFPHDYKSIEVLEGDGKAVASVRLITYSEGSS 65
           TL  EV++K+ A KF            KA P + +S ++ EGD   V S+    Y     
Sbjct: 168 TLETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNYVHDGE 227

Query: 66  LVKESKERIEAVDEEKMTVSYSVIEGDLLKYYKSFKGHIVVSPKEDGSGSSVKWSCEFEK 125
             K +KERIEAVD EK  +++ VIEGDL+K YKSF   I V+PK  GSGS V W  E+EK
Sbjct: 228 -AKVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSGSVVHWHFEYEK 287

Query: 126 ASEEVPDPHVIKDFVVKNFLEVDAYALLQ 155
            +EEV  P  +  F V+   E+D + L +
Sbjct: 288 INEEVAHPETLLQFAVEVSKEIDEHLLAE 315

[Close]

BLAST of Lag0040021 vs. ExPASy Swiss-Prot
Match: Q9SSK9 (MLP-like protein 28 OS=Arabidopsis thaliana OX=3702 GN=MLP28 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 8.6e-20
Identity = 58/150 (38.67%), Postives = 81/150 (54.00%), Query Frame = 0


Query: 5   GTLSVEVDVKSIARKFWTCIRDSTTIFPKAFPHDYKSIEVLEGDGKAVASVRLITYSEGS 64
           G L  +V++K+ A KF            KA P + +  ++ EGD   V S+    Y    
Sbjct: 24  GKLETDVEIKASADKFHHMFAGKPHHVSKASPGNIQGCDLHEGDWGTVGSIVFWNYVHDG 83

Query: 65  SLVKESKERIEAVDEEKMTVSYSVIEGDLLKYYKSFKGHIVVSPKEDGSGSSVKWSCEFE 124
              K +KERIEAV+ +K  +++ VIEGDL+K YKSF   I V+PK  G GS V W  E+E
Sbjct: 84  E-AKVAKERIEAVEPDKNLITFRVIEGDLMKEYKSFLLTIQVTPKPGGPGSIVHWHLEYE 143

Query: 125 KASEEVPDPHVIKDFVVKNFLEVDAYALLQ 155
           K SEEV  P  +  F V+   E+D + L +
Sbjct: 144 KISEEVAHPETLLQFCVEVSKEIDEHLLAE 172

[Close]

BLAST of Lag0040021 vs. ExPASy Swiss-Prot
Match: Q941R6 (MLP-like protein 31 OS=Arabidopsis thaliana OX=3702 GN=MLP31 PE=2 SV=2)

HSP 1 Score: 96.7 bits (239), Expect = 2.5e-19
Identity = 58/148 (39.19%), Postives = 78/148 (52.70%), Query Frame = 0


Query: 5   GTLSVEVDVKSIARKFWTCIRDSTTIFPKAFPHDYKSIEVLEGDGKAVASVRLITYSEGS 64
           G L  ++++K+ A KF            KA P   +  E+ EGD   V S+    Y    
Sbjct: 22  GKLETDIEIKASAGKFHHMFAGRPHHVSKATPGKIQGCELHEGDWGKVGSIVFWNYVHDG 81

Query: 65  SLVKESKERIEAVDEEKMTVSYSVIEGDLLKYYKSFKGHIVVSPKEDGSGSSVKWSCEFE 124
              K +KERIEAV+ EK  +++ VIEGDLLK YKSF   I V+PK  G GS V W  E+E
Sbjct: 82  E-AKVAKERIEAVEPEKNLITFRVIEGDLLKEYKSFVITIQVTPKRGGPGSVVHWHVEYE 141

Query: 125 KASEEVPDPHVIKDFVVKNFLEVDAYAL 153
           K  ++V  P    DF V+   E+D + L
Sbjct: 142 KIDDKVAHPETFLDFCVEVSKEIDEHLL 168

[Close]

BLAST of Lag0040021 vs. ExPASy Swiss-Prot
Match: Q9SSK5 (MLP-like protein 43 OS=Arabidopsis thaliana OX=3702 GN=MLP43 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 2.5e-19
Identity = 56/150 (37.33%), Postives = 79/150 (52.67%), Query Frame = 0


Query: 5   GTLSVEVDVKSIARKFWTCIRDSTTIFPKAFPHDYKSIEVLEGDGKAVASVRLITYSEGS 64
           G L  EV++K+ A+KF     +      KA P      E+ EGD   V S+ +  Y    
Sbjct: 9   GKLETEVEIKASAKKFHHMFTERPHHVSKATPDKIHGCELHEGDWGKVGSIVIWKYVHDG 68

Query: 65  SLVKESKERIEAVDEEKMTVSYSVIEGDLLKYYKSFKGHIVVSPKEDGSGSSVKWSCEFE 124
            L    K +IEAVD EK  +++ V+EGDL+  YKSF   + V+PK+  SGS   W  E+E
Sbjct: 69  KLT-VGKNKIEAVDPEKNLITFKVLEGDLMNEYKSFAFTLQVTPKQGESGSIAHWHLEYE 128

Query: 125 KASEEVPDPHVIKDFVVKNFLEVDAYALLQ 155
           K SEEV  P  +  F V+   E+D + L +
Sbjct: 129 KISEEVAHPETLLQFCVEISKEIDEHLLAE 157

The following BLAST results are available for this feature:

BLAST of Lag0040021 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022942327.1	5.1e-70	89.61	MLP-like protein 423 [Cucurbita moschata] >KAG6600129.1 MLP-like protein 423, pa...	[more]
XP_023553168.1	6.7e-70	88.96	MLP-like protein 423 [Cucurbita pepo subsp. pepo]	[more]
XP_022985665.1	1.9e-69	88.96	MLP-like protein 423 [Cucurbita maxima]	[more]
XP_008446239.1	8.2e-68	85.16	PREDICTED: MLP-like protein 423 [Cucumis melo] >KAA0034333.1 MLP-like protein 42...	[more]
XP_038892372.1	4.1e-67	85.16	MLP-like protein 423 [Benincasa hispida]	[more]

BLAST of Lag0040021 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VR4	8.3e-47	61.04	MLP-like protein 423 OS=Arabidopsis thaliana OX=3702 GN=MLP423 PE=2 SV=1	[more]
Q9SSK7	7.0e-22	41.61	MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1	[more]
Q9SSK9	8.6e-20	38.67	MLP-like protein 28 OS=Arabidopsis thaliana OX=3702 GN=MLP28 PE=1 SV=1	[more]
Q941R6	2.5e-19	39.19	MLP-like protein 31 OS=Arabidopsis thaliana OX=3702 GN=MLP31 PE=2 SV=2	[more]
Q9SSK5	2.5e-19	37.33	MLP-like protein 43 OS=Arabidopsis thaliana OX=3702 GN=MLP43 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024949	Bet v I type allergen	PRINTS	PR00634	BETALLERGEN	coord: 51..60 score: 17.98 coord: 26..36 score: 41.01 coord: 68..87 score: 35.37 coord: 87..100 score: 33.74 coord: 112..128 score: 23.78
IPR000916	Bet v I/Major latex protein	SMART	SM01037	Bet_v_1_2	coord: 2..154 e-value: 1.0E-55 score: 201.1
IPR000916	Bet v I/Major latex protein	PFAM	PF00407	Bet_v_1	coord: 4..152 e-value: 3.0E-38 score: 131.1
IPR023393	START-like domain superfamily	GENE3D	3.30.530.20		coord: 1..153 e-value: 3.7E-53 score: 181.7
None	No IPR available	PANTHER	PTHR31907	MLP-LIKE PROTEIN 423	coord: 6..152
None	No IPR available	PANTHER	PTHR31907:SF1	MLP-LIKE PROTEIN 423	coord: 6..152
None	No IPR available	CDD	cd07816	Bet_v1-like	coord: 5..154 e-value: 2.78457E-47 score: 148.492
None	No IPR available	SUPERFAMILY	55961	Bet v1-like	coord: 3..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0040021.1	Lag0040021.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009738	abscisic acid-activated signaling pathway
biological_process	GO:0006952	defense response
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0009607	response to biotic stimulus
cellular_component	GO:0005829	cytosol
molecular_function	GO:0010427	abscisic acid binding
molecular_function	GO:0004518	nuclease activity
molecular_function	GO:0004864	protein phosphatase inhibitor activity
molecular_function	GO:0038023	signaling receptor activity