Lag0040010 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040010
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionspermidine synthase-like
Locationchr13: 1520483 .. 1522962 (+)
RNA-Seq ExpressionLag0040010
SyntenyLag0040010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCGCAATTTTCCGTGGAGTCGGCGGCCGATGCGGCGGCGAAGAAACTGGAGAATGGGGTCTCCGCCTCACACCCCGATTGTATTTCCTCTGTAATTCCTGGATGGTTTTCTGAGATTAGCTCAATGTGGCCTGGTCAGTTTCTCGTCTCTAATCTCATATTTTCATCGTTTTGTCTGTGTTTGGCTGTTGAGATTCGTTAGATGATGAACAATATGATGGAATCTAATTTTTCTTTGAGCTTTTGAGAACTTTGGCTTTCGTTTTCGTTGAGAAAATCAAAAGATTTGATGTCATGCAGGAGAGGCTCACTCCTTGAAAGTGGAGAAGATTTTGTTCCAAGGGAAGTCTGATTACCAGAATGTTTTGGTCTTTCAGGTATTCTTATTTATATGAGGATGAGGATGGACTTTGTGATTTATTGTTTTGAACTATGATTTTTTCCCTTGATGTTGTGGTTTATGTGCCCTGCAGTCATCAACTTATGGGAAGGTTCTGGTTTTGGATGGGGTGATTCAGCTTACAGAGAGAGATGAATGTGCTTACCAAGAGATGATCACCCACCTTCCACTTTGCTCAATTCCAAACCCCAAAAAGGTTTTTCTCTTAGTGTAGTGTTTTGCAAATGTTTTAGGACTTGTATTTGGAGTCATAAGTTATGTCATACATACTCTGAATGATGGAAAGTCTAATACCTTTGATTCTTGAGAGACTTAATGAGAAGCAGGGCATGTCAGTGACCTTTTTGTTGGTATTTTGTCATAAATATCTGTAGCAAAGTTTCATACAAAAGTTAAGTTTTTGTTGGTATTTTGTCATAAATATCTGGGATGGATAGAATGGAGGATTTGAACCTACAACCTGTCAGGAAGTAAGGATAGGATGCTTCAACTGTTGAGCTATGTTTATGTTCGCTAAGTTTTAATCTTATTTCATAGAATGTTTTCAATTATAAGTGCAACGAATTTTAGTCGTTTGAGCTTATGTTATACTTCATTGGGAAATTGGTGGGTGGAGGGTCTGAAAAAGAATGATTTTTTGCCTCTAATTTGCTCAAAAGCTCTCACATGCGTTAGGATTTTTATTTCACAAGTTTCAGTGAATTTTGAGTATCATATTGGTTGGCGTTAATTTGATTATTTGAGTTTTCTTTTCTTGTTTTCTCACTCTATTATTGTTATTAGGTTCTAGTTATTGGTGGAGGAGATGGCGGTGTTTTGCGAGAGGTGGCTCGCCATTCATCTGTTGAGCAGATAGATATCTGTGAAATTGACAAGATGGTAGTTGACGTAAGTAGTAACTTTCCAGCATTATCAGTTATTTTGTTGCCTTTTCGAGTACAAACGTGATATTATGATATGCGAGGTGCTGATATGCTTATGACAGATGTGTGGAACTAATTCTGATTTATGATTAAATACTATTGCCTGATACCTTTGCAGGTTTCCAAAGAATTTTTCCCTCGCGTAGCTGTTGGGTACGAGGATCCCCGCGTCACCCTTCATGTTGGTGATGGTATATGATCAATCATTTTCTTGTCAGCTTCCCCTCAACCTCTTCTTTCTGTTTAATATCTCAACTGTGTTTATCGGTGTGCTTCTCGTGTTGCAGGCGTTGCATTTCTGAAGGCTGTTCCTGAAGGCACTTATGATGCAATTATAGTGGATTCTTCTGATCCTATTGGTAATTATATCTTTTTTTCATTCCAAGAGAAACATGGAACTATATGTTGGATTCTTTGAGAAAATGAAAATCAATTGCCATGATACAGGTCCTGCACAAGAGCTTTTTGAGAAGCCATTTTTTGCTTCAGTTGCCAAAGCTCTTCGATCAGGAGGTGTTGTGTGCACTCAGGCAGAGAGCATTTGGCTTCACATGAAAATCATCGAAGACATTGCTACGAACTGCCGCCAAATATTCAAAGGATCTGTCAACTATGCATGGACTACAGTTCCTACATATCCAAGGTACACACACCTAACAATTCGACATTTCCTTGTTCAGCTTATCGGCATCTCTTGGGTTATATTCGACCAGTTCTCTTAGAATTTATACGAAATCATCTTTTTATCGTTCTCTCCTGTTGAATGTGGCTTTCTCTAAACATCCCTCTGGTTTGTGTTTCAGTGGAGTGATCGGGTTTATGCTCTGCTCAACCGAGGGACCTGCGGTTGATTTCAAGCATCCAGTCAACCCAGTAGAGGTGAATGGCATCGATACTGCGGGGAATCCGCTCAAGTTTTACAACTCCGAGGTGGGATCTGTTGTCGTCATCCTTCAAATTTGCTTAAGATCACAATGTCCTTCTTTTGACTTCGAACTAAAGCTTCTCTTTTTCCCTGTTCTTTTGTTGAAAATCTTCAGATTCACTCAGCAGCTTTCTGTTTGCCTTCATTTGCGAAGAAGATTATCGACTCAAAAGCAAAATGAACATCTTCCGCGCAGCGTTGAAGAAGGCAGAAATTGGCTGTCTTAG

mRNA sequence

ATGGCTGCGCAATTTTCCGTGGAGTCGGCGGCCGATGCGGCGGCGAAGAAACTGGAGAATGGGGTCTCCGCCTCACACCCCGATTGTATTTCCTCTGTAATTCCTGGATGGTTTTCTGAGATTAGCTCAATGTGGCCTGGAGAGGCTCACTCCTTGAAAGTGGAGAAGATTTTGTTCCAAGGGAAGTCTGATTACCAGAATGTTTTGGTCTTTCAGTCATCAACTTATGGGAAGGTTCTGGTTTTGGATGGGGTGATTCAGCTTACAGAGAGAGATGAATGTGCTTACCAAGAGATGATCACCCACCTTCCACTTTGCTCAATTCCAAACCCCAAAAAGGTTCTAGTTATTGGTGGAGGAGATGGCGGTGTTTTGCGAGAGGTGGCTCGCCATTCATCTGTTGAGCAGATAGATATCTGTGAAATTGACAAGATGGTAGTTGACGTTTCCAAAGAATTTTTCCCTCGCGTAGCTGTTGGGTACGAGGATCCCCGCGTCACCCTTCATGTTGGTGATGGCGTTGCATTTCTGAAGGCTGTTCCTGAAGGCACTTATGATGCAATTATAGTGGATTCTTCTGATCCTATTGGTCCTGCACAAGAGCTTTTTGAGAAGCCATTTTTTGCTTCAGTTGCCAAAGCTCTTCGATCAGGAGGTGTTGTGTGCACTCAGGCAGAGAGCATTTGGCTTCACATGAAAATCATCGAAGACATTGCTACGAACTGCCGCCAAATATTCAAAGGATCTGTCAACTATGCATGGACTACAGTTCCTACATATCCAAGTGGAGTGATCGGGTTTATGCTCTGCTCAACCGAGGGACCTGCGGTTGATTTCAAGCATCCAGTCAACCCAGTAGAGGTGAATGGCATCGATACTGCGGGGAATCCGCTCAAGTTTTACAACTCCGAGGTGGGATCTGTTGTCGTCATCCTTCAAATTTGCTTAAGATCACAATGTCCTTCTTTTGACTTCGAACTAAAGCTTCTCTTTTTCCCTGTTCTTTTGTTGAAAATCTTCAGATTCACTCAGCAGCTTTCTGTTTGCCTTCATTTGCGAAGAAGATTATCGACTCAAAAGCAAAATGAACATCTTCCGCGCAGCGTTGAAGAAGGCAGAAATTGGCTGTCTTAG

Coding sequence (CDS)

ATGGCTGCGCAATTTTCCGTGGAGTCGGCGGCCGATGCGGCGGCGAAGAAACTGGAGAATGGGGTCTCCGCCTCACACCCCGATTGTATTTCCTCTGTAATTCCTGGATGGTTTTCTGAGATTAGCTCAATGTGGCCTGGAGAGGCTCACTCCTTGAAAGTGGAGAAGATTTTGTTCCAAGGGAAGTCTGATTACCAGAATGTTTTGGTCTTTCAGTCATCAACTTATGGGAAGGTTCTGGTTTTGGATGGGGTGATTCAGCTTACAGAGAGAGATGAATGTGCTTACCAAGAGATGATCACCCACCTTCCACTTTGCTCAATTCCAAACCCCAAAAAGGTTCTAGTTATTGGTGGAGGAGATGGCGGTGTTTTGCGAGAGGTGGCTCGCCATTCATCTGTTGAGCAGATAGATATCTGTGAAATTGACAAGATGGTAGTTGACGTTTCCAAAGAATTTTTCCCTCGCGTAGCTGTTGGGTACGAGGATCCCCGCGTCACCCTTCATGTTGGTGATGGCGTTGCATTTCTGAAGGCTGTTCCTGAAGGCACTTATGATGCAATTATAGTGGATTCTTCTGATCCTATTGGTCCTGCACAAGAGCTTTTTGAGAAGCCATTTTTTGCTTCAGTTGCCAAAGCTCTTCGATCAGGAGGTGTTGTGTGCACTCAGGCAGAGAGCATTTGGCTTCACATGAAAATCATCGAAGACATTGCTACGAACTGCCGCCAAATATTCAAAGGATCTGTCAACTATGCATGGACTACAGTTCCTACATATCCAAGTGGAGTGATCGGGTTTATGCTCTGCTCAACCGAGGGACCTGCGGTTGATTTCAAGCATCCAGTCAACCCAGTAGAGGTGAATGGCATCGATACTGCGGGGAATCCGCTCAAGTTTTACAACTCCGAGGTGGGATCTGTTGTCGTCATCCTTCAAATTTGCTTAAGATCACAATGTCCTTCTTTTGACTTCGAACTAAAGCTTCTCTTTTTCCCTGTTCTTTTGTTGAAAATCTTCAGATTCACTCAGCAGCTTTCTGTTTGCCTTCATTTGCGAAGAAGATTATCGACTCAAAAGCAAAATGAACATCTTCCGCGCAGCGTTGAAGAAGGCAGAAATTGGCTGTCTTAG

Protein sequence

MAAQFSVESAADAAAKKLENGVSASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKILFQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYDAIIVDSSDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGIDTAGNPLKFYNSEVGSVVVILQICLRSQCPSFDFELKLLFFPVLLLKIFRFTQQLSVCLHLRRRLSTQKQNEHLPRSVEEGRNWLS
Homology
BLAST of Lag0040010 vs. NCBI nr
Match: XP_038893068.1 (spermidine synthase [Benincasa hispida])

HSP 1 Score: 589.3 bits (1518), Expect = 2.3e-164
Identity = 290/314 (92.36%), Postives = 298/314 (94.90%), Query Frame = 0

Query: 1   MAAQFSVESAADAAAKK--LENGVSASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKIL 60
           MAA++SV SAAD AAKK  ++NGVSASHPD ISSVIPGWFSEIS MWPGEAHSLKVEK+L
Sbjct: 1   MAAEYSVGSAADVAAKKPEIQNGVSASHPDSISSVIPGWFSEISPMWPGEAHSLKVEKVL 60

Query: 61  FQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIG 120
           FQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIG
Sbjct: 61  FQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIG 120

Query: 121 GGDGGVLREVARHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLK 180
           GGDGGVLREVARHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLK
Sbjct: 121 GGDGGVLREVARHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLK 180

Query: 181 AVPEGTYDAIIVDSSDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDI 240
           AVPEGTYDAIIVDSSDPIGPAQELFEKPFFASVAKALR GGVVCTQAESIWLHM IIEDI
Sbjct: 181 AVPEGTYDAIIVDSSDPIGPAQELFEKPFFASVAKALRPGGVVCTQAESIWLHMHIIEDI 240

Query: 241 ATNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGIDTAGNPL 300
            TNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGP VDFKHPVNPVEVNGID+A +PL
Sbjct: 241 VTNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPPVDFKHPVNPVEVNGIDSAKSPL 300

Query: 301 KFYNSEVGSVVVIL 313
           KFYNSE+ S    L
Sbjct: 301 KFYNSEIHSAAFCL 314

BLAST of Lag0040010 vs. NCBI nr
Match: KAG6573749.1 (hypothetical protein SDJN03_27636, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 580.1 bits (1494), Expect = 1.4e-161
Identity = 283/306 (92.48%), Postives = 289/306 (94.44%), Query Frame = 0

Query: 7   VESAADAAAKKLENGVSASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKILFQGKSDYQ 66
           V  AA AAAKKLENGVSAS  D ISSVIPGWFSEIS MWPGEAHSLKVEKILFQGKSDYQ
Sbjct: 69  VVMAAAAAAKKLENGVSASESDGISSVIPGWFSEISQMWPGEAHSLKVEKILFQGKSDYQ 128

Query: 67  NVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLR 126
           NV+VFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLR
Sbjct: 129 NVMVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLR 188

Query: 127 EVARHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYD 186
           EVARHSSVE+IDICEIDKMVVDVSKEFFPRVA+GYEDPRVTLHVGDGVAFLKAVPEGTYD
Sbjct: 189 EVARHSSVEKIDICEIDKMVVDVSKEFFPRVAIGYEDPRVTLHVGDGVAFLKAVPEGTYD 248

Query: 187 AIIVDSSDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIF 246
           A+IVDSSDPIGPAQELFEKPFFASVAKALR GGVVCTQAESIWLHMKIIEDI TNCRQ+F
Sbjct: 249 AVIVDSSDPIGPAQELFEKPFFASVAKALRPGGVVCTQAESIWLHMKIIEDIVTNCRQVF 308

Query: 247 KGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGIDTAGNPLKFYNSEVG 306
           KGSVNYAWTTVPTYPSGVIGFMLCSTEGP VDFKHPVNPVEVNGIDT  NPLKFYNSE+ 
Sbjct: 309 KGSVNYAWTTVPTYPSGVIGFMLCSTEGPPVDFKHPVNPVEVNGIDTPNNPLKFYNSEIH 368

Query: 307 SVVVIL 313
           S    L
Sbjct: 369 SAAFCL 374

BLAST of Lag0040010 vs. NCBI nr
Match: XP_022945657.1 (spermidine synthase-like [Cucurbita moschata])

HSP 1 Score: 580.1 bits (1494), Expect = 1.4e-161
Identity = 282/304 (92.76%), Postives = 289/304 (95.07%), Query Frame = 0

Query: 9   SAADAAAKKLENGVSASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKILFQGKSDYQNV 68
           +AA AAAKKLENGVSAS  D ISSVIPGWFSEIS MWPGEAHSLKVEKILFQGKSDYQNV
Sbjct: 2   AAAAAAAKKLENGVSASESDGISSVIPGWFSEISQMWPGEAHSLKVEKILFQGKSDYQNV 61

Query: 69  LVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREV 128
           +VFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREV
Sbjct: 62  MVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREV 121

Query: 129 ARHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYDAI 188
           ARHSSVE+IDICEIDKMVVDVSKEFFPRVA+GYEDPRVTLHVGDGVAFLKAVPEGTYDA+
Sbjct: 122 ARHSSVEKIDICEIDKMVVDVSKEFFPRVAIGYEDPRVTLHVGDGVAFLKAVPEGTYDAV 181

Query: 189 IVDSSDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIFKG 248
           IVDSSDPIGPAQELFEKPFFASVAKALR GGVVCTQAESIWLHMKIIEDI TNCRQ+FKG
Sbjct: 182 IVDSSDPIGPAQELFEKPFFASVAKALRPGGVVCTQAESIWLHMKIIEDIVTNCRQVFKG 241

Query: 249 SVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGIDTAGNPLKFYNSEVGSV 308
           SVNYAWTTVPTYPSGVIGFMLCSTEGP VDFKHPVNPVEVNGIDT  NPLKFYNSE+ S 
Sbjct: 242 SVNYAWTTVPTYPSGVIGFMLCSTEGPPVDFKHPVNPVEVNGIDTPNNPLKFYNSEIHSA 301

Query: 309 VVIL 313
              L
Sbjct: 302 AFCL 305

BLAST of Lag0040010 vs. NCBI nr
Match: XP_023542334.1 (spermidine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 577.4 bits (1487), Expect = 9.0e-161
Identity = 281/303 (92.74%), Postives = 287/303 (94.72%), Query Frame = 0

Query: 10  AADAAAKKLENGVSASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKILFQGKSDYQNVL 69
           AA AAAKK ENGVSAS  D ISSVIPGWFSEIS MWPGEAHSLKVEKILFQGKSDYQNV+
Sbjct: 2   AAAAAAKKQENGVSASESDGISSVIPGWFSEISQMWPGEAHSLKVEKILFQGKSDYQNVM 61

Query: 70  VFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVA 129
           VFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVA
Sbjct: 62  VFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVA 121

Query: 130 RHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYDAII 189
           RHSSVE+IDICEIDKMVVDVSKEFFPRVA+GYEDPRVTLHVGDGVAFLKAVPEGTYDA+I
Sbjct: 122 RHSSVEKIDICEIDKMVVDVSKEFFPRVAIGYEDPRVTLHVGDGVAFLKAVPEGTYDAVI 181

Query: 190 VDSSDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIFKGS 249
           VDSSDPIGPAQELFEKPFFASVAKALR GGVVCTQAESIWLHMKIIEDI TNCRQ+FKGS
Sbjct: 182 VDSSDPIGPAQELFEKPFFASVAKALRPGGVVCTQAESIWLHMKIIEDIVTNCRQVFKGS 241

Query: 250 VNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGIDTAGNPLKFYNSEVGSVV 309
           VNYAWTTVPTYPSGVIGFMLCSTEGP VDFKHPVNPVEVNGIDT  NPLKFYNSE+ S  
Sbjct: 242 VNYAWTTVPTYPSGVIGFMLCSTEGPPVDFKHPVNPVEVNGIDTQNNPLKFYNSEIHSAA 301

Query: 310 VIL 313
             L
Sbjct: 302 FCL 304

BLAST of Lag0040010 vs. NCBI nr
Match: XP_023004424.1 (spermidine synthase [Cucurbita maxima])

HSP 1 Score: 576.6 bits (1485), Expect = 1.5e-160
Identity = 282/314 (89.81%), Postives = 295/314 (93.95%), Query Frame = 0

Query: 1   MAAQFSVESAADAAAKK--LENGVSASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKIL 60
           M+A+  V SAADAAAKK  +ENGVSAS PD ISSVIPGWFSEIS +WPGEAHSLKVEK+L
Sbjct: 1   MSAEHFVGSAADAAAKKPEIENGVSASQPDSISSVIPGWFSEISPLWPGEAHSLKVEKVL 60

Query: 61  FQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIG 120
           FQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIG
Sbjct: 61  FQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIG 120

Query: 121 GGDGGVLREVARHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLK 180
           GGDGGVLREVARHSSVEQIDICEIDKMVV+VSKEFFPRVAVG+EDPRVTLH+GDGVAFLK
Sbjct: 121 GGDGGVLREVARHSSVEQIDICEIDKMVVEVSKEFFPRVAVGFEDPRVTLHIGDGVAFLK 180

Query: 181 AVPEGTYDAIIVDSSDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDI 240
           AVPEGTYDA+IVDSSDPIGPAQELFEKPFFASVAKALR GGVVCTQAESIWLHM IIEDI
Sbjct: 181 AVPEGTYDAVIVDSSDPIGPAQELFEKPFFASVAKALRPGGVVCTQAESIWLHMHIIEDI 240

Query: 241 ATNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGIDTAGNPL 300
            TNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGP +DFKHPVNPVEVNGIDT  +PL
Sbjct: 241 VTNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPPLDFKHPVNPVEVNGIDTVKSPL 300

Query: 301 KFYNSEVGSVVVIL 313
           KFYNSE+ +    L
Sbjct: 301 KFYNSEIHTAAFCL 314

BLAST of Lag0040010 vs. ExPASy Swiss-Prot
Match: O82147 (Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 2.0e-147
Identity = 253/291 (86.94%), Postives = 264/291 (90.72%), Query Frame = 0

Query: 23  SASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKILFQGKSDYQNVLVFQSSTYGKVLVL 82
           +A  PD +SSVIPGWFSEIS MWPGEAHSLKVEKILFQGKSDYQNV+VFQSSTYGKVLVL
Sbjct: 13  AAQLPDGVSSVIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLVL 72

Query: 83  DGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHSSVEQIDICEI 142
           DGVIQLTERDECAYQEMI HLPLCSIP+PKKVLVIGGGDGGVLREVARH SVEQIDICEI
Sbjct: 73  DGVIQLTERDECAYQEMIAHLPLCSIPSPKKVLVIGGGDGGVLREVARHLSVEQIDICEI 132

Query: 143 DKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYDAIIVDSSDPIGPAQEL 202
           DKMVVDVSK+FFP VAVG+EDPRV LH+GDGVAFLKAVPEGTYDAIIVDSSDPIGPAQEL
Sbjct: 133 DKMVVDVSKQFFPDVAVGFEDPRVVLHIGDGVAFLKAVPEGTYDAIIVDSSDPIGPAQEL 192

Query: 203 FEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIFKGSVNYAWTTVPTYPS 262
           FEKPFF SVAKALR GGVVCTQAESIWLHM IIEDI  NCRQIFKGSVNYAWTTVPTYPS
Sbjct: 193 FEKPFFESVAKALRPGGVVCTQAESIWLHMHIIEDIVANCRQIFKGSVNYAWTTVPTYPS 252

Query: 263 GVIGFMLCSTEGPAVDFKHPVNPVEVN-GIDTAGNPLKFYNSEVGSVVVIL 313
           GVIGFMLCSTEGP VDFKHP+NP++ N G      PLKFYNSE+ S    L
Sbjct: 253 GVIGFMLCSTEGPPVDFKHPINPIDANDGRSKTMKPLKFYNSEIHSAAFCL 303

BLAST of Lag0040010 vs. ExPASy Swiss-Prot
Match: Q9ZTR1 (Spermidine synthase 1 OS=Pisum sativum OX=3888 GN=SPDSYN1 PE=2 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 2.5e-145
Identity = 252/321 (78.50%), Postives = 277/321 (86.29%), Query Frame = 0

Query: 1   MAAQFSVESAADAAAKKLE----NGVSAS-----HPDCISSVIPGWFSEISSMWPGEAHS 60
           MAA  +   + D+  K+      NGVS +      P+ +SSVIPGWFSEIS MWPGEAHS
Sbjct: 1   MAAPENTLHSTDSPLKRQREDEVNGVSDTLSKEPQPNGLSSVIPGWFSEISPMWPGEAHS 60

Query: 61  LKVEKILFQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNP 120
           LKVEKILFQGKSDYQ+V+VFQS+TYGKVL+LDGVIQLTERDECAYQEMITHLPLCSIPNP
Sbjct: 61  LKVEKILFQGKSDYQDVMVFQSATYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNP 120

Query: 121 KKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVG 180
           KKVLVIGGGDGGVLREVARHSSVE+IDICEIDKMVVDVSKE+FP +AVG+ DPRVTL++G
Sbjct: 121 KKVLVIGGGDGGVLREVARHSSVEKIDICEIDKMVVDVSKEYFPDIAVGFADPRVTLNIG 180

Query: 181 DGVAFLKAVPEGTYDAIIVDSSDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLH 240
           DGVAFLKA PEGTYDA+IVDSSDPIGPAQELFEKPFF SVA+ALR GGVVCTQAESIWLH
Sbjct: 181 DGVAFLKAAPEGTYDAVIVDSSDPIGPAQELFEKPFFESVARALRPGGVVCTQAESIWLH 240

Query: 241 MKIIEDIATNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGI 300
           M IIEDI  NCRQ+FKGSVNYAWTTVPTYPSG+IGFMLCSTEGP+VDFKHPVNP++ N  
Sbjct: 241 MHIIEDIVVNCRQVFKGSVNYAWTTVPTYPSGMIGFMLCSTEGPSVDFKHPVNPIDENDS 300

Query: 301 DTAGNPLKFYNSEVGSVVVIL 313
             A  PLKFYN E+ S    L
Sbjct: 301 QQAARPLKFYNREIHSAAFCL 321

BLAST of Lag0040010 vs. ExPASy Swiss-Prot
Match: Q96556 (Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.2e-144
Identity = 244/279 (87.46%), Postives = 260/279 (93.19%), Query Frame = 0

Query: 30  ISSVIPGWFSEISSMWPGEAHSLKVEKILFQGKSDYQNVLVFQSSTYGKVLVLDGVIQLT 89
           ISS++PGWFSEIS +WPGEAHSLKVEKILFQGKSDYQNV+VFQSSTYGKVL+LDGVIQLT
Sbjct: 12  ISSILPGWFSEISPLWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLILDGVIQLT 71

Query: 90  ERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDV 149
           ERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREV+RHSSVEQIDICEIDKMV+DV
Sbjct: 72  ERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVIDV 131

Query: 150 SKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYDAIIVDSSDPIGPAQELFEKPFFA 209
           SK+FFP VA+GYEDPRV LHVGDGVAFLK V EGTYDA+IVDSSDPIGPAQELFEKPFF 
Sbjct: 132 SKQFFPNVAIGYEDPRVKLHVGDGVAFLKFVAEGTYDAVIVDSSDPIGPAQELFEKPFFE 191

Query: 210 SVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIFKGSVNYAWTTVPTYPSGVIGFML 269
           SVA+ALR GGVVCTQAESIWLHM IIEDI  NCRQIFKGSVNYAWTTVPTYPSGVIGFML
Sbjct: 192 SVARALRPGGVVCTQAESIWLHMHIIEDIVANCRQIFKGSVNYAWTTVPTYPSGVIGFML 251

Query: 270 CSTEGPAVDFKHPVNPVEVNGIDT-AGNPLKFYNSEVGS 308
           CSTEGPAVDFK+P+NPV+ +   T    PLKFYNSE+ S
Sbjct: 252 CSTEGPAVDFKNPINPVDADDSHTKTRGPLKFYNSEIHS 290

BLAST of Lag0040010 vs. ExPASy Swiss-Prot
Match: O48659 (Spermidine synthase 2 OS=Hyoscyamus niger OX=4079 PE=2 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.2e-144
Identity = 244/279 (87.46%), Postives = 261/279 (93.55%), Query Frame = 0

Query: 30  ISSVIPGWFSEISSMWPGEAHSLKVEKILFQGKSDYQNVLVFQSSTYGKVLVLDGVIQLT 89
           ISS++PGWFSEIS +WPGEAHSLKVEKILFQGKSDYQNV+VFQSSTYGKVLVLDGVIQLT
Sbjct: 12  ISSILPGWFSEISPLWPGEAHSLKVEKILFQGKSDYQNVVVFQSSTYGKVLVLDGVIQLT 71

Query: 90  ERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDV 149
           ERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREV+RHSSVEQIDICEIDKMV+DV
Sbjct: 72  ERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVIDV 131

Query: 150 SKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYDAIIVDSSDPIGPAQELFEKPFFA 209
           SK+FFP VA+GYEDPRV LHVGDGVAFLK VPEGTYDA+IVDSSDPIGPAQELFEKPFF 
Sbjct: 132 SKQFFPNVAIGYEDPRVKLHVGDGVAFLKNVPEGTYDAVIVDSSDPIGPAQELFEKPFFE 191

Query: 210 SVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIFKGSVNYAWTTVPTYPSGVIGFML 269
           SVA+AL  GGVVCTQAESIWLHM IIEDI +NCRQIFKGSVNYAWTTVPTYPSGVIGFML
Sbjct: 192 SVARALCPGGVVCTQAESIWLHMHIIEDIVSNCRQIFKGSVNYAWTTVPTYPSGVIGFML 251

Query: 270 CSTEGPAVDFKHPVNPVEVNGIDT-AGNPLKFYNSEVGS 308
           CSTEGPAVDFK+P+NP++ +   T    PLKFYNSE+ S
Sbjct: 252 CSTEGPAVDFKNPINPIDADDSHTKTRGPLKFYNSEIHS 290

BLAST of Lag0040010 vs. ExPASy Swiss-Prot
Match: Q9ZS45 (Spermidine synthase OS=Solanum lycopersicum OX=4081 GN=SPDSYN PE=2 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 3.3e-142
Identity = 244/293 (83.28%), Postives = 261/293 (89.08%), Query Frame = 0

Query: 14  AAKKLENGVSASHPD-CISSVIPGWFSEISSMWPGEAHSLKVEKILFQGKSDYQNVLVFQ 73
           AA    NG         ISSV+PGWFSEIS +WPGEAHSLKVEKILFQGKSDYQNVLVFQ
Sbjct: 30  AANNSNNGCEKEESSPYISSVLPGWFSEISPLWPGEAHSLKVEKILFQGKSDYQNVLVFQ 89

Query: 74  SSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHS 133
           SSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREV+RHS
Sbjct: 90  SSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVSRHS 149

Query: 134 SVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYDAIIVDS 193
           SVEQIDICEIDKMVV+V+K+FFP VAVGYEDPRV LH+GDGVAFLK VP GTYDA+IVDS
Sbjct: 150 SVEQIDICEIDKMVVEVAKQFFPDVAVGYEDPRVNLHIGDGVAFLKNVPAGTYDAVIVDS 209

Query: 194 SDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIFKGSVNY 253
           SDPIGPAQELFEKPFF S+AKALR GGVV TQAESIWLHM IIE+I  NCRQIFKGSVNY
Sbjct: 210 SDPIGPAQELFEKPFFESIAKALRPGGVVSTQAESIWLHMHIIEEIVANCRQIFKGSVNY 269

Query: 254 AWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGIDTAGNPLKFYNSEV 306
           AWTTVPTYPSG+IGFMLCSTEGPAVDFK+P+NP++      +  PLKFYNSE+
Sbjct: 270 AWTTVPTYPSGMIGFMLCSTEGPAVDFKNPINPIDDESPAKSIEPLKFYNSEI 322

BLAST of Lag0040010 vs. ExPASy TrEMBL
Match: A0A6J1G1J3 (spermidine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111449832 PE=3 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 6.7e-162
Identity = 282/304 (92.76%), Postives = 289/304 (95.07%), Query Frame = 0

Query: 9   SAADAAAKKLENGVSASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKILFQGKSDYQNV 68
           +AA AAAKKLENGVSAS  D ISSVIPGWFSEIS MWPGEAHSLKVEKILFQGKSDYQNV
Sbjct: 2   AAAAAAAKKLENGVSASESDGISSVIPGWFSEISQMWPGEAHSLKVEKILFQGKSDYQNV 61

Query: 69  LVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREV 128
           +VFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREV
Sbjct: 62  MVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREV 121

Query: 129 ARHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYDAI 188
           ARHSSVE+IDICEIDKMVVDVSKEFFPRVA+GYEDPRVTLHVGDGVAFLKAVPEGTYDA+
Sbjct: 122 ARHSSVEKIDICEIDKMVVDVSKEFFPRVAIGYEDPRVTLHVGDGVAFLKAVPEGTYDAV 181

Query: 189 IVDSSDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIFKG 248
           IVDSSDPIGPAQELFEKPFFASVAKALR GGVVCTQAESIWLHMKIIEDI TNCRQ+FKG
Sbjct: 182 IVDSSDPIGPAQELFEKPFFASVAKALRPGGVVCTQAESIWLHMKIIEDIVTNCRQVFKG 241

Query: 249 SVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGIDTAGNPLKFYNSEVGSV 308
           SVNYAWTTVPTYPSGVIGFMLCSTEGP VDFKHPVNPVEVNGIDT  NPLKFYNSE+ S 
Sbjct: 242 SVNYAWTTVPTYPSGVIGFMLCSTEGPPVDFKHPVNPVEVNGIDTPNNPLKFYNSEIHSA 301

Query: 309 VVIL 313
              L
Sbjct: 302 AFCL 305

BLAST of Lag0040010 vs. ExPASy TrEMBL
Match: A0A6J1KW89 (spermidine synthase OS=Cucurbita maxima OX=3661 GN=LOC111497722 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 7.4e-161
Identity = 282/314 (89.81%), Postives = 295/314 (93.95%), Query Frame = 0

Query: 1   MAAQFSVESAADAAAKK--LENGVSASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKIL 60
           M+A+  V SAADAAAKK  +ENGVSAS PD ISSVIPGWFSEIS +WPGEAHSLKVEK+L
Sbjct: 1   MSAEHFVGSAADAAAKKPEIENGVSASQPDSISSVIPGWFSEISPLWPGEAHSLKVEKVL 60

Query: 61  FQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIG 120
           FQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIG
Sbjct: 61  FQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIG 120

Query: 121 GGDGGVLREVARHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLK 180
           GGDGGVLREVARHSSVEQIDICEIDKMVV+VSKEFFPRVAVG+EDPRVTLH+GDGVAFLK
Sbjct: 121 GGDGGVLREVARHSSVEQIDICEIDKMVVEVSKEFFPRVAVGFEDPRVTLHIGDGVAFLK 180

Query: 181 AVPEGTYDAIIVDSSDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDI 240
           AVPEGTYDA+IVDSSDPIGPAQELFEKPFFASVAKALR GGVVCTQAESIWLHM IIEDI
Sbjct: 181 AVPEGTYDAVIVDSSDPIGPAQELFEKPFFASVAKALRPGGVVCTQAESIWLHMHIIEDI 240

Query: 241 ATNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGIDTAGNPL 300
            TNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGP +DFKHPVNPVEVNGIDT  +PL
Sbjct: 241 VTNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPPLDFKHPVNPVEVNGIDTVKSPL 300

Query: 301 KFYNSEVGSVVVIL 313
           KFYNSE+ +    L
Sbjct: 301 KFYNSEIHTAAFCL 314

BLAST of Lag0040010 vs. ExPASy TrEMBL
Match: A0A6J1HQJ5 (spermidine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111466453 PE=3 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 3.1e-159
Identity = 278/303 (91.75%), Postives = 285/303 (94.06%), Query Frame = 0

Query: 10  AADAAAKKLENGVSASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKILFQGKSDYQNVL 69
           AA A AKK ENGVSAS  D ISSVIPGWFSEIS MWPGEAHSLKVEKILFQGKSDYQNV+
Sbjct: 2   AAAAVAKKQENGVSASESDGISSVIPGWFSEISQMWPGEAHSLKVEKILFQGKSDYQNVM 61

Query: 70  VFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVA 129
           VFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVA
Sbjct: 62  VFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVA 121

Query: 130 RHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYDAII 189
           RHSSVE+IDICEIDKMVVDVSKEFFPRVA+G+EDPRVTLHVGDGVAFLKAVPEGTYDA+I
Sbjct: 122 RHSSVEKIDICEIDKMVVDVSKEFFPRVAIGFEDPRVTLHVGDGVAFLKAVPEGTYDAVI 181

Query: 190 VDSSDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIFKGS 249
           VDSSDPIGPAQELFEKPFFASVAKALR GGVVCTQAESIWLHMKIIEDI TNCRQ+FKGS
Sbjct: 182 VDSSDPIGPAQELFEKPFFASVAKALRPGGVVCTQAESIWLHMKIIEDIVTNCRQVFKGS 241

Query: 250 VNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGIDTAGNPLKFYNSEVGSVV 309
           VNYAWTTVPTYPSGVIGFMLCSTEGP VDFKHPVNPVEVNGIDT  N LKFYNSE+ S  
Sbjct: 242 VNYAWTTVPTYPSGVIGFMLCSTEGPPVDFKHPVNPVEVNGIDTPNNSLKFYNSEIHSAA 301

Query: 310 VIL 313
             L
Sbjct: 302 FCL 304

BLAST of Lag0040010 vs. ExPASy TrEMBL
Match: A0A6J1GYE0 (spermidine synthase OS=Cucurbita moschata OX=3662 GN=LOC111458321 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 5.3e-159
Identity = 280/315 (88.89%), Postives = 293/315 (93.02%), Query Frame = 0

Query: 1   MAAQFSVESAADAAAKK---LENGVSASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKI 60
           M+A+  V SAADAAA K   +ENGVSAS PD ISSVIPGWFSEIS MWPGEAHSLKVEK+
Sbjct: 1   MSAEHFVGSAADAAAAKKPEIENGVSASLPDSISSVIPGWFSEISPMWPGEAHSLKVEKV 60

Query: 61  LFQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVI 120
           LFQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVI
Sbjct: 61  LFQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVI 120

Query: 121 GGGDGGVLREVARHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFL 180
           GGGDGGVLREVARHSSVE+IDICEIDKMVV+VSKEFFPRVAVG+EDPRVTLH+GDGVAFL
Sbjct: 121 GGGDGGVLREVARHSSVERIDICEIDKMVVEVSKEFFPRVAVGFEDPRVTLHIGDGVAFL 180

Query: 181 KAVPEGTYDAIIVDSSDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIED 240
           KAVPEGTYDA+IVDSSDPIGPAQELFEKPFFASVAKALR GGVVCTQAESIWLHM IIED
Sbjct: 181 KAVPEGTYDAVIVDSSDPIGPAQELFEKPFFASVAKALRPGGVVCTQAESIWLHMHIIED 240

Query: 241 IATNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGIDTAGNP 300
           I TNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGP +DFKHPVNPVEVNGID   +P
Sbjct: 241 IVTNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPPLDFKHPVNPVEVNGIDNVKSP 300

Query: 301 LKFYNSEVGSVVVIL 313
           LKFYNSE+ +    L
Sbjct: 301 LKFYNSEIHTAAFCL 315

BLAST of Lag0040010 vs. ExPASy TrEMBL
Match: A0A6J1DB45 (spermidine synthase OS=Momordica charantia OX=3673 GN=LOC111019077 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.5e-158
Identity = 285/323 (88.24%), Postives = 291/323 (90.09%), Query Frame = 0

Query: 1   MAAQFSVESAADAAAKK-LENGVSA----------SHPDCISSVIPGWFSEISSMWPGEA 60
           MAA+  V SAADAAAKK  ENGVSA            PDCISSVIPGWFSEIS MWPGEA
Sbjct: 1   MAAEHGVGSAADAAAKKPEENGVSAPALVTATEGKKEPDCISSVIPGWFSEISPMWPGEA 60

Query: 61  HSLKVEKILFQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIP 120
           HSLKVEKILFQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMI HLPLCSIP
Sbjct: 61  HSLKVEKILFQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMIAHLPLCSIP 120

Query: 121 NPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLH 180
           NPKKVLVIGGGDGGVLREV+RHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLH
Sbjct: 121 NPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLH 180

Query: 181 VGDGVAFLKAVPEGTYDAIIVDSSDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIW 240
           VGDGVAFLKAVPEGTYDAIIVDSSDPIGPAQELFEKPFFASVAKALR GGVVCTQAESIW
Sbjct: 181 VGDGVAFLKAVPEGTYDAIIVDSSDPIGPAQELFEKPFFASVAKALRPGGVVCTQAESIW 240

Query: 241 LHMKIIEDIATNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVN 300
           LHM IIEDI TNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGP VDFKHPVNP+E N
Sbjct: 241 LHMHIIEDIVTNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPPVDFKHPVNPIEDN 300

Query: 301 GIDTAGNPLKFYNSEVGSVVVIL 313
           G +TA  PLKFYNSE+ +    L
Sbjct: 301 GSETAKGPLKFYNSEIHTAAFCL 323

BLAST of Lag0040010 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 506.1 bits (1302), Expect = 2.4e-143
Identity = 239/300 (79.67%), Postives = 267/300 (89.00%), Query Frame = 0

Query: 13  AAAKKLENGVSASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKILFQGKSDYQNVLVFQ 72
           AA  + ENG     P C S+VIPGWFSE+S MWPGEAHSLKVEK+LFQGKSDYQ+V+VFQ
Sbjct: 67  AATMETENGDQKKEPACFSTVIPGWFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQ 126

Query: 73  SSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHS 132
           S+TYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARH+
Sbjct: 127 SATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHA 186

Query: 133 SVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYDAIIVDS 192
           S+EQID+CEIDKMVVDVSK+FFP VA+GYEDPRV L +GDGVAFLK   EG+YDA+IVDS
Sbjct: 187 SIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDS 246

Query: 193 SDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIFKGSVNY 252
           SDPIGPA+ELFEKPFF SVA+ALR GGVVCTQAES+WLHM IIEDI +NCR+IFKGSVNY
Sbjct: 247 SDPIGPAKELFEKPFFQSVARALRPGGVVCTQAESLWLHMDIIEDIVSNCREIFKGSVNY 306

Query: 253 AWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGIDTAGNPLKFYNSEVGSVVVIL 312
           AWT+VPTYPSGVIGFMLCSTEGP VDFKHP+NP++ +   + G PLKFYN+E+ S    L
Sbjct: 307 AWTSVPTYPSGVIGFMLCSTEGPDVDFKHPLNPIDESSSKSNG-PLKFYNAEIHSAAFCL 365

BLAST of Lag0040010 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 488.0 bits (1255), Expect = 6.7e-138
Identity = 237/307 (77.20%), Postives = 263/307 (85.67%), Query Frame = 0

Query: 8   ESAADAAAKKLEN-GVSASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKILFQGKSDYQ 67
           E   +  A + EN G     P C+SS+IPGWFSEIS MWPGEAHSLKVEKILFQGKSDYQ
Sbjct: 21  EEDNNGGAMETENGGGEIKEPSCMSSIIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQ 80

Query: 68  NVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLR 127
           +V+VFQS+TYGKVLVLDGVIQLTERDECAYQEMITHLPLCSI NPKKVLVIGGGDGGVLR
Sbjct: 81  DVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISNPKKVLVIGGGDGGVLR 140

Query: 128 EVARHSSVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYD 187
           EVARHSSVEQIDICEIDKMVVDV+K++FP VAVGYEDPRV L +GDGVAFLK   EGTYD
Sbjct: 141 EVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLIIGDGVAFLKNAAEGTYD 200

Query: 188 AIIVDSSDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIF 247
           A+IVDSSDPIGPA+ELFEKPFF SV +ALR GGVVCTQAES+WLHM IIEDI +NCR IF
Sbjct: 201 AVIVDSSDPIGPAKELFEKPFFESVNRALRPGGVVCTQAESLWLHMDIIEDIVSNCRDIF 260

Query: 248 KGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVEVNGIDTAGN-PLKFYNSEV 307
           KGSVNYAWT+VPTYPSGVIGFMLCS+EGP VDFK PV+ ++ +      + PLK+YN+E+
Sbjct: 261 KGSVNYAWTSVPTYPSGVIGFMLCSSEGPQVDFKKPVSLIDTDESSIKSHCPLKYYNAEI 320

Query: 308 GSVVVIL 313
            S    L
Sbjct: 321 HSAAFCL 327

BLAST of Lag0040010 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 441.0 bits (1133), Expect = 9.4e-124
Identity = 207/249 (83.13%), Postives = 228/249 (91.57%), Query Frame = 0

Query: 13  AAAKKLENGVSASHPDCISSVIPGWFSEISSMWPGEAHSLKVEKILFQGKSDYQNVLVFQ 72
           AA  + ENG     P C S+VIPGWFSE+S MWPGEAHSLKVEK+LFQGKSDYQ+V+VFQ
Sbjct: 67  AATMETENGDQKKEPACFSTVIPGWFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQ 126

Query: 73  SSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHS 132
           S+TYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARH+
Sbjct: 127 SATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHA 186

Query: 133 SVEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYDAIIVDS 192
           S+EQID+CEIDKMVVDVSK+FFP VA+GYEDPRV L +GDGVAFLK   EG+YDA+IVDS
Sbjct: 187 SIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDS 246

Query: 193 SDPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIFKGSVNY 252
           SDPIGPA+ELFEKPFF SVA+ALR GGVVCTQAES+WLHM IIEDI +NCR+IFKGSVNY
Sbjct: 247 SDPIGPAKELFEKPFFQSVARALRPGGVVCTQAESLWLHMDIIEDIVSNCREIFKGSVNY 306

Query: 253 AWTTVPTYP 262
           AWT+VPTYP
Sbjct: 307 AWTSVPTYP 315

BLAST of Lag0040010 vs. TAIR 10
Match: AT5G53120.1 (spermidine synthase 3 )

HSP 1 Score: 421.8 bits (1083), Expect = 5.9e-118
Identity = 201/310 (64.84%), Postives = 245/310 (79.03%), Query Frame = 0

Query: 29  CISSVIPGWFSE---------------ISSMWPGEAHSLKVEKILFQGKSDYQNVLVFQS 88
           C S+V+ GWFSE                + MWPGEAHSLKVEK+LF+ KSD+Q VLVF+S
Sbjct: 47  CHSTVVSGWFSEPHPRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFES 106

Query: 89  STYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHSS 148
           +TYGKVLVLDG++QLTE+DECAYQEMI HLPLCSI +PK VLV+GGGDGGVLRE++RHSS
Sbjct: 107 ATYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSS 166

Query: 149 VEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYDAIIVDSS 208
           VE IDICEIDKMV+DVSK+FFP +AVG++DPRV LH+GD   FL+  PEG YDAIIVDSS
Sbjct: 167 VEVIDICEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSS 226

Query: 209 DPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIFKGSVNYA 268
           DP+GPA  L EKPFF ++A+AL+ GGV+C  AES+WLH  +IED+ + CRQ FK SV+YA
Sbjct: 227 DPVGPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYA 286

Query: 269 WTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVE-VNGIDTAGNPLKFYNSEVGSVVVIL 323
           W++VPTYPSGVIGF+LCSTEGPAVDFK+P+NP+E ++G  T    LKFYNS++      L
Sbjct: 287 WSSVPTYPSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFAL 346

BLAST of Lag0040010 vs. TAIR 10
Match: AT5G53120.2 (spermidine synthase 3 )

HSP 1 Score: 421.8 bits (1083), Expect = 5.9e-118
Identity = 201/310 (64.84%), Postives = 245/310 (79.03%), Query Frame = 0

Query: 29  CISSVIPGWFSE---------------ISSMWPGEAHSLKVEKILFQGKSDYQNVLVFQS 88
           C S+V+ GWFSE                + MWPGEAHSLKVEK+LF+ KSD+Q VLVF+S
Sbjct: 47  CHSTVVSGWFSEPHPRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFES 106

Query: 89  STYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHSS 148
           +TYGKVLVLDG++QLTE+DECAYQEMI HLPLCSI +PK VLV+GGGDGGVLRE++RHSS
Sbjct: 107 ATYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSS 166

Query: 149 VEQIDICEIDKMVVDVSKEFFPRVAVGYEDPRVTLHVGDGVAFLKAVPEGTYDAIIVDSS 208
           VE IDICEIDKMV+DVSK+FFP +AVG++DPRV LH+GD   FL+  PEG YDAIIVDSS
Sbjct: 167 VEVIDICEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSS 226

Query: 209 DPIGPAQELFEKPFFASVAKALRSGGVVCTQAESIWLHMKIIEDIATNCRQIFKGSVNYA 268
           DP+GPA  L EKPFF ++A+AL+ GGV+C  AES+WLH  +IED+ + CRQ FK SV+YA
Sbjct: 227 DPVGPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYA 286

Query: 269 WTTVPTYPSGVIGFMLCSTEGPAVDFKHPVNPVE-VNGIDTAGNPLKFYNSEVGSVVVIL 323
           W++VPTYPSGVIGF+LCSTEGPAVDFK+P+NP+E ++G  T    LKFYNS++      L
Sbjct: 287 WSSVPTYPSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFAL 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893068.12.3e-16492.36spermidine synthase [Benincasa hispida][more]
KAG6573749.11.4e-16192.48hypothetical protein SDJN03_27636, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022945657.11.4e-16192.76spermidine synthase-like [Cucurbita moschata][more]
XP_023542334.19.0e-16192.74spermidine synthase-like [Cucurbita pepo subsp. pepo][more]
XP_023004424.11.5e-16089.81spermidine synthase [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O821472.0e-14786.94Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1[more]
Q9ZTR12.5e-14578.50Spermidine synthase 1 OS=Pisum sativum OX=3888 GN=SPDSYN1 PE=2 SV=1[more]
Q965561.2e-14487.46Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1[more]
O486591.2e-14487.46Spermidine synthase 2 OS=Hyoscyamus niger OX=4079 PE=2 SV=1[more]
Q9ZS453.3e-14283.28Spermidine synthase OS=Solanum lycopersicum OX=4081 GN=SPDSYN PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G1J36.7e-16292.76spermidine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111449832 PE=3 SV=1[more]
A0A6J1KW897.4e-16189.81spermidine synthase OS=Cucurbita maxima OX=3661 GN=LOC111497722 PE=3 SV=1[more]
A0A6J1HQJ53.1e-15991.75spermidine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111466453 PE=3 SV=1[more]
A0A6J1GYE05.3e-15988.89spermidine synthase OS=Cucurbita moschata OX=3662 GN=LOC111458321 PE=3 SV=1[more]
A0A6J1DB451.5e-15888.24spermidine synthase OS=Momordica charantia OX=3673 GN=LOC111019077 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G23820.12.4e-14379.67spermidine synthase 1 [more]
AT1G70310.16.7e-13877.20spermidine synthase 2 [more]
AT1G23820.29.4e-12483.13spermidine synthase 1 [more]
AT5G53120.15.9e-11864.84spermidine synthase 3 [more]
AT5G53120.25.9e-11864.84spermidine synthase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 88..321
e-value: 3.6E-90
score: 303.5
NoneNo IPR availablePFAMPF01564Spermine_synthcoord: 93..280
e-value: 1.2E-73
score: 246.6
NoneNo IPR availablePANTHERPTHR11558:SF50SPERMIDINE SYNTHASE-RELATEDcoord: 20..312
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 113..224
e-value: 3.05524E-11
score: 57.825
IPR001045Spermidine/spermine synthasesTIGRFAMTIGR00417TIGR00417coord: 37..307
e-value: 3.9E-93
score: 309.6
IPR001045Spermidine/spermine synthasesPANTHERPTHR11558SPERMIDINE/SPERMINE SYNTHASEcoord: 20..312
IPR001045Spermidine/spermine synthasesHAMAPMF_00198Spermidine_synthcoord: 36..341
score: 33.587807
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 36..90
e-value: 1.6E-24
score: 85.5
IPR037163Spermidine synthase, tetramerisation domain superfamilyGENE3D2.30.140.10Spermidine synthase, tetramerisation domaincoord: 28..87
e-value: 1.1E-30
score: 107.8
IPR030373Polyamine biosynthesis domain, conserved sitePROSITEPS01330PABS_1coord: 114..127
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 35..272
score: 106.126244
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 33..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040010.1Lag0040010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008295 spermidine biosynthetic process
molecular_function GO:0004766 spermidine synthase activity
molecular_function GO:0003824 catalytic activity