Lag0039986 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0039986
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	DNA-directed RNA polymerase subunit 10-like protein
Location	chr13: 1335332 .. 1336063 (-)
RNA-Seq Expression	Lag0039986
Synteny	Lag0039986

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAATTCCGGTGCGCTGTTTCACGTGCGGCAAGGTACTGATTCCGCTTCTTCATTGTCGATCGCCGTTCTTGAAGCTTCGAAAATCCTTCGCCTGCTCTGTTTCGTATTCTGTATAATTTTGCTCTGTTTTTTCAGGTGATCGGTAACAAATGGGACACTTATCTTGAACTTCTACAAGCAGACTATCCGGAAGGGTAAGAGAGAACGAACGTCTCTTTCTTCTGCTCCTTCCTCTTCTTAAATTTGAATCGCTTTCCTTTGATTTTGATCCTACCATCGCGAACACGATCCTTCCGATTTCTTTCTCGTATTTTGAATTTTTCGAAACGCATTTTGATTTGGAAGACCTAATTTCTTCAATATTAAGGCTCTGCGTTTAGAAATAGTTATGAATTTGTTAGAAGTAGCAAGGAAGTGGAAGGATTGAAAGATGATGAAATAGTAATTGAGGATGCAGGGAGGCATTGGATATGTTAGGGCTAGTTCGATACTGCTGCCGGCGGATGCTTATGACTCATGTGGATCTCATAGAGAAGCTCTTGAATTACAACAGTAATCTCTTTTTCTCTGGATTTTATTGATGATTTAGAAGTGTTTCTGATGGATTTATGTGTTGTTGACGCTGATTATTTTGCAGCCATGGATAAGTCTGAAATCGTGTAGCTGATGAGGAAGCCTTTCGATATGCCAACAAGAAGAATGCTGCAATGCAATGCAATGCTGTGTAA

mRNA sequence

ATGATAATTCCGGTGCGCTGTTTCACGTGCGGCAAGGTGATCGGTAACAAATGGGACACTTATCTTGAACTTCTACAAGCAGACTATCCGGAAGGGGAGGCATTGGATATGTTAGGGCTAGTTCGATACTGCTGCCGGCGGATGCTTATGACTCATGTGGATCTCATAGAGAAGCTCTTGAATTACAACACTGATGAGGAAGCCTTTCGATATGCCAACAAGAAGAATGCTGCAATGCAATGCAATGCTGTGTAA

Coding sequence (CDS)

ATGATAATTCCGGTGCGCTGTTTCACGTGCGGCAAGGTGATCGGTAACAAATGGGACACTTATCTTGAACTTCTACAAGCAGACTATCCGGAAGGGGAGGCATTGGATATGTTAGGGCTAGTTCGATACTGCTGCCGGCGGATGCTTATGACTCATGTGGATCTCATAGAGAAGCTCTTGAATTACAACACTGATGAGGAAGCCTTTCGATATGCCAACAAGAAGAATGCTGCAATGCAATGCAATGCTGTGTAA

Protein sequence

MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLLNYNTDEEAFRYANKKNAAMQCNAV

Homology

[Close]

BLAST of Lag0039986 vs. NCBI nr
Match: XP_038893151.1 (DNA-directed RNA polymerase subunit 10-like protein [Benincasa hispida])

HSP 1 Score: 142.1 bits (357), Expect = 2.1e-30
Identity = 64/68 (94.12%), Postives = 67/68 (98.53%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT +++
Sbjct: 61 NYNTMDKS 68

[Close]

BLAST of Lag0039986 vs. NCBI nr
Match: XP_004135479.1 (DNA-directed RNA polymerase subunit 10-like protein [Cucumis sativus] >KGN51793.1 hypothetical protein Csa_009052 [Cucumis sativus])

HSP 1 Score: 141.0 bits (354), Expect = 4.8e-30
Identity = 63/68 (92.65%), Postives = 67/68 (98.53%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWDTYL+LLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT +++
Sbjct: 61 NYNTMDKS 68

[Close]

BLAST of Lag0039986 vs. NCBI nr
Match: XP_022151052.1 (DNA-directed RNA polymerase subunit 10-like protein [Momordica charantia])

HSP 1 Score: 139.4 bits (350), Expect = 1.4e-29
Identity = 62/68 (91.18%), Postives = 67/68 (98.53%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD+YLELLQADYPEGEALD+LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT +++
Sbjct: 61 NYNTMDKS 68

[Close]

BLAST of Lag0039986 vs. NCBI nr
Match: XP_008446194.1 (PREDICTED: DNA-directed RNA polymerase subunit 10-like protein [Cucumis melo] >KAA0034305.1 DNA-directed RNA polymerase subunit 10-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 138.7 bits (348), Expect = 2.4e-29
Identity = 62/68 (91.18%), Postives = 66/68 (97.06%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWDTYL+LLQADYPEGEALD LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYPEGEALDTLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT +++
Sbjct: 61 NYNTMDKS 68

[Close]

BLAST of Lag0039986 vs. NCBI nr
Match: XP_040374532.1 (DNA-directed RNA polymerase subunit 10-like protein [Rosa chinensis] >PRQ40975.1 putative DNA-directed RNA polymerase transcription regulator Homeodomain-LIKE family [Rosa chinensis])

HSP 1 Score: 138.3 bits (347), Expect = 3.1e-29
Identity = 62/68 (91.18%), Postives = 66/68 (97.06%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWDTYL+LLQADYPEG+ALD LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYPEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT E++
Sbjct: 61 NYNTLEKS 68

[Close]

BLAST of Lag0039986 vs. ExPASy Swiss-Prot
Match: Q9SYA6 (DNA-directed RNA polymerase subunit 10-like protein OS=Arabidopsis thaliana OX=3702 GN=NRPB10L PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 1.5e-31
Identity = 61/68 (89.71%), Postives = 65/68 (95.59%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MI+PVRCFTCGKVIGNKWDTYLELLQADY EG+ALD LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIVPVRCFTCGKVIGNKWDTYLELLQADYAEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT E++
Sbjct: 61 NYNTMEKS 68

[Close]

BLAST of Lag0039986 vs. ExPASy Swiss-Prot
Match: Q39290 (DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Brassica napus OX=3708 PE=3 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 2.5e-29
Identity = 58/68 (85.29%), Postives = 62/68 (91.18%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD YL+LLQ DY EG+ALD L LVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDAYLDLLQLDYTEGDALDALNLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT E++
Sbjct: 61 NYNTLEKS 68

[Close]

BLAST of Lag0039986 vs. ExPASy Swiss-Prot
Match: Q8LFJ6 (DNA-directed RNA polymerases II, IV and V subunit 10 OS=Arabidopsis thaliana OX=3702 GN=NRPB10 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 5.5e-29
Identity = 58/68 (85.29%), Postives = 62/68 (91.18%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD YL+LLQ DY EG+ALD L LVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDQYLDLLQLDYTEGDALDALQLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT E++
Sbjct: 61 NYNTLEKS 68

[Close]

BLAST of Lag0039986 vs. ExPASy Swiss-Prot
Match: Q55AB6 (DNA-directed RNA polymerases I, II, and III subunit rpabc5 OS=Dictyostelium discoideum OX=44689 GN=polr2l PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 4.4e-26
Identity = 53/62 (85.48%), Postives = 55/62 (88.71%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD YL LLQ DY EG+ALD L L RYCCRRML+THVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDAYLSLLQIDYTEGDALDALCLKRYCCRRMLLTHVDLIEKLL 60

Query: 61 NY 63
          NY
Sbjct: 61 NY 62

[Close]

BLAST of Lag0039986 vs. ExPASy Swiss-Prot
Match: Q32P78 (DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Bos taurus OX=9913 GN=POLR2L PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 9.7e-26
Identity = 50/62 (80.65%), Postives = 56/62 (90.32%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGK++GNKW+ YL LLQA+Y EG+ALD LGL RYCCRRML+ HVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKIVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLAHVDLIEKLL 60

Query: 61 NY 63
          NY
Sbjct: 61 NY 62

The following BLAST results are available for this feature:

BLAST of Lag0039986 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893151.1	2.1e-30	94.12	DNA-directed RNA polymerase subunit 10-like protein [Benincasa hispida]	[more]
XP_004135479.1	4.8e-30	92.65	DNA-directed RNA polymerase subunit 10-like protein [Cucumis sativus] >KGN51793....	[more]
XP_022151052.1	1.4e-29	91.18	DNA-directed RNA polymerase subunit 10-like protein [Momordica charantia]	[more]
XP_008446194.1	2.4e-29	91.18	PREDICTED: DNA-directed RNA polymerase subunit 10-like protein [Cucumis melo] >K...	[more]
XP_040374532.1	3.1e-29	91.18	DNA-directed RNA polymerase subunit 10-like protein [Rosa chinensis] >PRQ40975.1...	[more]

BLAST of Lag0039986 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SYA6	1.5e-31	89.71	DNA-directed RNA polymerase subunit 10-like protein OS=Arabidopsis thaliana OX=3...	[more]
Q39290	2.5e-29	85.29	DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Brassica napus OX=...	[more]
Q8LFJ6	5.5e-29	85.29	DNA-directed RNA polymerases II, IV and V subunit 10 OS=Arabidopsis thaliana OX=...	[more]
Q55AB6	4.4e-26	85.48	DNA-directed RNA polymerases I, II, and III subunit rpabc5 OS=Dictyostelium disc...	[more]
Q32P78	9.7e-26	80.65	DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Bos taurus OX=9913...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000268	DNA-directed RNA polymerase, subunit N/Rpb10	PFAM	PF01194	RNA_pol_N	coord: 1..59 e-value: 2.8E-31 score: 107.6
IPR000268	DNA-directed RNA polymerase, subunit N/Rpb10	PANTHER	PTHR23431	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER	coord: 1..65
IPR000268	DNA-directed RNA polymerase, subunit N/Rpb10	HAMAP	MF_00250	RNApol_arch_N	coord: 1..63 score: 19.60733
None	No IPR available	GENE3D	1.10.10.60		coord: 1..67 e-value: 7.6E-39 score: 133.7
None	No IPR available	PANTHER	PTHR23431:SF6	SUBFAMILY NOT NAMED	coord: 1..65
IPR020789	RNA polymerases, subunit N, zinc binding site	PROSITE	PS01112	RNA_POL_N_8KD	coord: 2..11
IPR023580	RNA polymerase subunit RPB10	SUPERFAMILY	46924	RNA polymerase subunit RPB10	coord: 1..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0039986.1	Lag0039986.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006360	transcription by RNA polymerase I
biological_process	GO:0006366	transcription by RNA polymerase II
biological_process	GO:0042797	tRNA transcription by RNA polymerase III
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0008270	zinc ion binding