Lag0039986 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0039986
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDNA-directed RNA polymerase subunit 10-like protein
Locationchr13: 1335332 .. 1336063 (-)
RNA-Seq ExpressionLag0039986
SyntenyLag0039986
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAATTCCGGTGCGCTGTTTCACGTGCGGCAAGGTACTGATTCCGCTTCTTCATTGTCGATCGCCGTTCTTGAAGCTTCGAAAATCCTTCGCCTGCTCTGTTTCGTATTCTGTATAATTTTGCTCTGTTTTTTCAGGTGATCGGTAACAAATGGGACACTTATCTTGAACTTCTACAAGCAGACTATCCGGAAGGGTAAGAGAGAACGAACGTCTCTTTCTTCTGCTCCTTCCTCTTCTTAAATTTGAATCGCTTTCCTTTGATTTTGATCCTACCATCGCGAACACGATCCTTCCGATTTCTTTCTCGTATTTTGAATTTTTCGAAACGCATTTTGATTTGGAAGACCTAATTTCTTCAATATTAAGGCTCTGCGTTTAGAAATAGTTATGAATTTGTTAGAAGTAGCAAGGAAGTGGAAGGATTGAAAGATGATGAAATAGTAATTGAGGATGCAGGGAGGCATTGGATATGTTAGGGCTAGTTCGATACTGCTGCCGGCGGATGCTTATGACTCATGTGGATCTCATAGAGAAGCTCTTGAATTACAACAGTAATCTCTTTTTCTCTGGATTTTATTGATGATTTAGAAGTGTTTCTGATGGATTTATGTGTTGTTGACGCTGATTATTTTGCAGCCATGGATAAGTCTGAAATCGTGTAGCTGATGAGGAAGCCTTTCGATATGCCAACAAGAAGAATGCTGCAATGCAATGCAATGCTGTGTAA

mRNA sequence

ATGATAATTCCGGTGCGCTGTTTCACGTGCGGCAAGGTGATCGGTAACAAATGGGACACTTATCTTGAACTTCTACAAGCAGACTATCCGGAAGGGGAGGCATTGGATATGTTAGGGCTAGTTCGATACTGCTGCCGGCGGATGCTTATGACTCATGTGGATCTCATAGAGAAGCTCTTGAATTACAACACTGATGAGGAAGCCTTTCGATATGCCAACAAGAAGAATGCTGCAATGCAATGCAATGCTGTGTAA

Coding sequence (CDS)

ATGATAATTCCGGTGCGCTGTTTCACGTGCGGCAAGGTGATCGGTAACAAATGGGACACTTATCTTGAACTTCTACAAGCAGACTATCCGGAAGGGGAGGCATTGGATATGTTAGGGCTAGTTCGATACTGCTGCCGGCGGATGCTTATGACTCATGTGGATCTCATAGAGAAGCTCTTGAATTACAACACTGATGAGGAAGCCTTTCGATATGCCAACAAGAAGAATGCTGCAATGCAATGCAATGCTGTGTAA

Protein sequence

MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLLNYNTDEEAFRYANKKNAAMQCNAV
Homology
BLAST of Lag0039986 vs. NCBI nr
Match: XP_038893151.1 (DNA-directed RNA polymerase subunit 10-like protein [Benincasa hispida])

HSP 1 Score: 142.1 bits (357), Expect = 2.1e-30
Identity = 64/68 (94.12%), Postives = 67/68 (98.53%), Query Frame = 0

Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT +++
Sbjct: 61 NYNTMDKS 68

BLAST of Lag0039986 vs. NCBI nr
Match: XP_004135479.1 (DNA-directed RNA polymerase subunit 10-like protein [Cucumis sativus] >KGN51793.1 hypothetical protein Csa_009052 [Cucumis sativus])

HSP 1 Score: 141.0 bits (354), Expect = 4.8e-30
Identity = 63/68 (92.65%), Postives = 67/68 (98.53%), Query Frame = 0

Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWDTYL+LLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT +++
Sbjct: 61 NYNTMDKS 68

BLAST of Lag0039986 vs. NCBI nr
Match: XP_022151052.1 (DNA-directed RNA polymerase subunit 10-like protein [Momordica charantia])

HSP 1 Score: 139.4 bits (350), Expect = 1.4e-29
Identity = 62/68 (91.18%), Postives = 67/68 (98.53%), Query Frame = 0

Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD+YLELLQADYPEGEALD+LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT +++
Sbjct: 61 NYNTMDKS 68

BLAST of Lag0039986 vs. NCBI nr
Match: XP_008446194.1 (PREDICTED: DNA-directed RNA polymerase subunit 10-like protein [Cucumis melo] >KAA0034305.1 DNA-directed RNA polymerase subunit 10-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 138.7 bits (348), Expect = 2.4e-29
Identity = 62/68 (91.18%), Postives = 66/68 (97.06%), Query Frame = 0

Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWDTYL+LLQADYPEGEALD LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYPEGEALDTLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT +++
Sbjct: 61 NYNTMDKS 68

BLAST of Lag0039986 vs. NCBI nr
Match: XP_040374532.1 (DNA-directed RNA polymerase subunit 10-like protein [Rosa chinensis] >PRQ40975.1 putative DNA-directed RNA polymerase transcription regulator Homeodomain-LIKE family [Rosa chinensis])

HSP 1 Score: 138.3 bits (347), Expect = 3.1e-29
Identity = 62/68 (91.18%), Postives = 66/68 (97.06%), Query Frame = 0

Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWDTYL+LLQADYPEG+ALD LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYPEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT E++
Sbjct: 61 NYNTLEKS 68

BLAST of Lag0039986 vs. ExPASy Swiss-Prot
Match: Q9SYA6 (DNA-directed RNA polymerase subunit 10-like protein OS=Arabidopsis thaliana OX=3702 GN=NRPB10L PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 1.5e-31
Identity = 61/68 (89.71%), Postives = 65/68 (95.59%), Query Frame = 0

Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MI+PVRCFTCGKVIGNKWDTYLELLQADY EG+ALD LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIVPVRCFTCGKVIGNKWDTYLELLQADYAEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT E++
Sbjct: 61 NYNTMEKS 68

BLAST of Lag0039986 vs. ExPASy Swiss-Prot
Match: Q39290 (DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Brassica napus OX=3708 PE=3 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 2.5e-29
Identity = 58/68 (85.29%), Postives = 62/68 (91.18%), Query Frame = 0

Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD YL+LLQ DY EG+ALD L LVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDAYLDLLQLDYTEGDALDALNLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT E++
Sbjct: 61 NYNTLEKS 68

BLAST of Lag0039986 vs. ExPASy Swiss-Prot
Match: Q8LFJ6 (DNA-directed RNA polymerases II, IV and V subunit 10 OS=Arabidopsis thaliana OX=3702 GN=NRPB10 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 5.5e-29
Identity = 58/68 (85.29%), Postives = 62/68 (91.18%), Query Frame = 0

Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD YL+LLQ DY EG+ALD L LVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDQYLDLLQLDYTEGDALDALQLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNTDEEA 69
          NYNT E++
Sbjct: 61 NYNTLEKS 68

BLAST of Lag0039986 vs. ExPASy Swiss-Prot
Match: Q55AB6 (DNA-directed RNA polymerases I, II, and III subunit rpabc5 OS=Dictyostelium discoideum OX=44689 GN=polr2l PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 4.4e-26
Identity = 53/62 (85.48%), Postives = 55/62 (88.71%), Query Frame = 0

Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD YL LLQ DY EG+ALD L L RYCCRRML+THVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDAYLSLLQIDYTEGDALDALCLKRYCCRRMLLTHVDLIEKLL 60

Query: 61 NY 63
          NY
Sbjct: 61 NY 62

BLAST of Lag0039986 vs. ExPASy Swiss-Prot
Match: Q32P78 (DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Bos taurus OX=9913 GN=POLR2L PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 9.7e-26
Identity = 50/62 (80.65%), Postives = 56/62 (90.32%), Query Frame = 0

Query: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGK++GNKW+ YL LLQA+Y EG+ALD LGL RYCCRRML+ HVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKIVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLAHVDLIEKLL 60

Query: 61 NY 63
          NY
Sbjct: 61 NY 62

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893151.12.1e-3094.12DNA-directed RNA polymerase subunit 10-like protein [Benincasa hispida][more]
XP_004135479.14.8e-3092.65DNA-directed RNA polymerase subunit 10-like protein [Cucumis sativus] >KGN51793.... [more]
XP_022151052.11.4e-2991.18DNA-directed RNA polymerase subunit 10-like protein [Momordica charantia][more]
XP_008446194.12.4e-2991.18PREDICTED: DNA-directed RNA polymerase subunit 10-like protein [Cucumis melo] >K... [more]
XP_040374532.13.1e-2991.18DNA-directed RNA polymerase subunit 10-like protein [Rosa chinensis] >PRQ40975.1... [more]
Match NameE-valueIdentityDescription
Q9SYA61.5e-3189.71DNA-directed RNA polymerase subunit 10-like protein OS=Arabidopsis thaliana OX=3... [more]
Q392902.5e-2985.29DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Brassica napus OX=... [more]
Q8LFJ65.5e-2985.29DNA-directed RNA polymerases II, IV and V subunit 10 OS=Arabidopsis thaliana OX=... [more]
Q55AB64.4e-2685.48DNA-directed RNA polymerases I, II, and III subunit rpabc5 OS=Dictyostelium disc... [more]
Q32P789.7e-2680.65DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Bos taurus OX=9913... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000268DNA-directed RNA polymerase, subunit N/Rpb10PFAMPF01194RNA_pol_Ncoord: 1..59
e-value: 2.8E-31
score: 107.6
IPR000268DNA-directed RNA polymerase, subunit N/Rpb10PANTHERPTHR23431DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBERcoord: 1..65
IPR000268DNA-directed RNA polymerase, subunit N/Rpb10HAMAPMF_00250RNApol_arch_Ncoord: 1..63
score: 19.60733
NoneNo IPR availableGENE3D1.10.10.60coord: 1..67
e-value: 7.6E-39
score: 133.7
NoneNo IPR availablePANTHERPTHR23431:SF6SUBFAMILY NOT NAMEDcoord: 1..65
IPR020789RNA polymerases, subunit N, zinc binding sitePROSITEPS01112RNA_POL_N_8KDcoord: 2..11
IPR023580RNA polymerase subunit RPB10SUPERFAMILY46924RNA polymerase subunit RPB10coord: 1..63

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0039986.1Lag0039986.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006360 transcription by RNA polymerase I
biological_process GO:0006366 transcription by RNA polymerase II
biological_process GO:0042797 tRNA transcription by RNA polymerase III
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0008270 zinc ion binding