Lag0039973 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0039973
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Pectinesterase inhibitor-like
Location	chr13: 1248391 .. 1248882 (-)
RNA-Seq Expression	Lag0039973
Synteny	Lag0039973

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGAAAAGTACTTAGTTTCTTTGATCTCACTAGTCTTTCTCGCCGCCGTTGCCATCTTTGCCGGCCAAGCTCAGGGAGCCGACCTCTGCGCCAAAGCTGAATTCGTGCCGCTGTGCCAGTCCGTCGTGAAAGGCGCCACCGATCCAAAAGCCGCCTTGAAGACCTCCATCGAATACCTCATCTCCGAAACGAAAAAAGCGGTTGCAATGAGCGAGAAGCTGGGAAAAACGCAGCATCTGACGGTGTGCAAAGAGAACTTAAACTCCGCCATTGACGACCTGCACAAAAGCCTCGAGTATTTGGAGTCCGGCGACAAGAATGGCCTGAAAACCGTACTCACGGGGGCCTCGACCTACTACGTCACGTGCGACGACGCGGTGGCGGAAGGCGGCATCGTGCAGCTGGCTGCCGACCTTTTGGCCGCCGATACTAAGCTGCATCGAATGGCTAACAATTGCTTGCACTTGTCCTCTTTGCTGGTCGTCTAA

mRNA sequence

ATGGCCGAAAAGTACTTAGTTTCTTTGATCTCACTAGTCTTTCTCGCCGCCGTTGCCATCTTTGCCGGCCAAGCTCAGGGAGCCGACCTCTGCGCCAAAGCTGAATTCGTGCCGCTGTGCCAGTCCGTCGTGAAAGGCGCCACCGATCCAAAAGCCGCCTTGAAGACCTCCATCGAATACCTCATCTCCGAAACGAAAAAAGCGGTTGCAATGAGCGAGAAGCTGGGAAAAACGCAGCATCTGACGGTGTGCAAAGAGAACTTAAACTCCGCCATTGACGACCTGCACAAAAGCCTCGAGTATTTGGAGTCCGGCGACAAGAATGGCCTGAAAACCGTACTCACGGGGGCCTCGACCTACTACGTCACGTGCGACGACGCGGTGGCGGAAGGCGGCATCGTGCAGCTGGCTGCCGACCTTTTGGCCGCCGATACTAAGCTGCATCGAATGGCTAACAATTGCTTGCACTTGTCCTCTTTGCTGGTCGTCTAA

Coding sequence (CDS)

ATGGCCGAAAAGTACTTAGTTTCTTTGATCTCACTAGTCTTTCTCGCCGCCGTTGCCATCTTTGCCGGCCAAGCTCAGGGAGCCGACCTCTGCGCCAAAGCTGAATTCGTGCCGCTGTGCCAGTCCGTCGTGAAAGGCGCCACCGATCCAAAAGCCGCCTTGAAGACCTCCATCGAATACCTCATCTCCGAAACGAAAAAAGCGGTTGCAATGAGCGAGAAGCTGGGAAAAACGCAGCATCTGACGGTGTGCAAAGAGAACTTAAACTCCGCCATTGACGACCTGCACAAAAGCCTCGAGTATTTGGAGTCCGGCGACAAGAATGGCCTGAAAACCGTACTCACGGGGGCCTCGACCTACTACGTCACGTGCGACGACGCGGTGGCGGAAGGCGGCATCGTGCAGCTGGCTGCCGACCTTTTGGCCGCCGATACTAAGCTGCATCGAATGGCTAACAATTGCTTGCACTTGTCCTCTTTGCTGGTCGTCTAA

Protein sequence

MAEKYLVSLISLVFLAAVAIFAGQAQGADLCAKAEFVPLCQSVVKGATDPKAALKTSIEYLISETKKAVAMSEKLGKTQHLTVCKENLNSAIDDLHKSLEYLESGDKNGLKTVLTGASTYYVTCDDAVAEGGIVQLAADLLAADTKLHRMANNCLHLSSLLVV

Homology

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BLAST of Lag0039973 vs. NCBI nr
Match: XP_038891721.1 (uncharacterized protein LOC120081119 [Benincasa hispida])

HSP 1 Score: 160.6 bits (405), Expect = 1.1e-35
Identity = 88/166 (53.01%), Postives = 123/166 (74.10%), Query Frame = 0


Query: 1   MAEKYLVSLISLVFLAA--VAIFAGQAQGADLCAKAEFVPLCQSVVKGATDPKAALKTSI 60
           MAE  LVS  SLVFLAA  V +FAGQAQG D+C +A +VPLC+SVVKG +DP  A+  +I
Sbjct: 1   MAENRLVS-FSLVFLAAATVVLFAGQAQGIDICGEAGYVPLCRSVVKGVSDPAVAIVKAI 60

Query: 61  EYLISETKKAVAMSEKLGKTQHLTVCKENLNSAIDDLHKSLEYLESGDKNGLKTVLTGAS 120
           E+L+ ETK+A A S K+   Q + VC +N +SAIDDL K+LEYL+S DK  L+ +L+GA 
Sbjct: 61  EHLLFETKRAKAASVKMVNKQAINVCNQNYDSAIDDLEKALEYLKSNDKASLRVMLSGAL 120

Query: 121 TYYVTCDDAVAE---GGIVQLAADLLAADTKLHRMANNCLHLSSLL 162
           ++YV+C DAV E    G+V++ ++++  DT L ++A+NCLH++SLL
Sbjct: 121 SFYVSCTDAVMEVTGFGVVKMVSEIVKTDTTLKQLASNCLHIASLL 165

[Close]

BLAST of Lag0039973 vs. NCBI nr
Match: KAG7032299.1 (hypothetical protein SDJN02_06343, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 156.0 bits (393), Expect = 2.8e-34
Identity = 90/161 (55.90%), Postives = 111/161 (68.94%), Query Frame = 0


Query: 1   MAEKYLVSLISLVFLAAVAIFAGQAQGADLCAKAEFVPLCQSVVKGATDPKAALKTSIEY 60
           MAEK++V    L+F+AA A+ AGQA GAD+C KAE+  LC+S VKGAT+P A L+ +IE+
Sbjct: 1   MAEKHMV----LLFVAA-AVLAGQADGADICEKAEYGSLCRSSVKGATEPAAGLQAAIEH 60

Query: 61  LISETKKAVAMSEKLGKTQHLTVCKENLNSAIDDLHKSLEYLESGDKNGLKTVLTGASTY 120
           LISETK A   S KLG  + L VCK+N N AI DL KS++YL   DK  LK  L+ A T 
Sbjct: 61  LISETKHAKESSMKLGVMKCLDVCKQNYNDAITDLQKSVQYLTKNDKPSLKISLSAALTD 120

Query: 121 YVTCDDAVAEGGIVQLAADLLAADTKLHRMANNCLHLSSLL 162
           YVTCDDAV E G  +   DL++ D  L  MA NCLHL+SLL
Sbjct: 121 YVTCDDAVMESGNSKKVGDLMSVDVVLRHMAANCLHLASLL 156

[Close]

BLAST of Lag0039973 vs. NCBI nr
Match: XP_022957327.1 (pectinesterase 3-like [Cucurbita moschata])

HSP 1 Score: 155.6 bits (392), Expect = 3.6e-34
Identity = 88/161 (54.66%), Postives = 111/161 (68.94%), Query Frame = 0


Query: 1   MAEKYLVSLISLVFLAAVAIFAGQAQGADLCAKAEFVPLCQSVVKGATDPKAALKTSIEY 60
           MAEK++V    L+F+AA A+ AGQA GAD+C KAE+  LC+S VKGAT+P A L+ +IE+
Sbjct: 1   MAEKHMV----LLFVAA-AVLAGQADGADICEKAEYASLCRSSVKGATEPAAGLQAAIEH 60

Query: 61  LISETKKAVAMSEKLGKTQHLTVCKENLNSAIDDLHKSLEYLESGDKNGLKTVLTGASTY 120
           LISETK A   S KLG  + L VCK+N N A+ DL +S++YL   DK  LK  L+ A T 
Sbjct: 61  LISETKHAKESSMKLGVMKCLDVCKQNYNDAVTDLQQSVQYLTKNDKPSLKISLSAALTD 120

Query: 121 YVTCDDAVAEGGIVQLAADLLAADTKLHRMANNCLHLSSLL 162
           YVTCDDAV E G  +   DL++ D  L  MA NCLHL+SLL
Sbjct: 121 YVTCDDAVMESGNSKKVGDLMSVDVMLRHMAANCLHLASLL 156

[Close]

BLAST of Lag0039973 vs. NCBI nr
Match: XP_022983388.1 (uncharacterized protein LOC111481993 [Cucurbita maxima])

HSP 1 Score: 155.2 bits (391), Expect = 4.8e-34
Identity = 89/167 (53.29%), Postives = 120/167 (71.86%), Query Frame = 0


Query: 1   MAEKYLVSLISLVFLAAVAIFA----GQAQGADLCAKAEFVPLCQSVVKGATDPKAALKT 60
           MAE  LVS  SL+FLAAVA       GQA G+D+C KA++V LC+  VKG+ DP+ ALK 
Sbjct: 1   MAENRLVS-FSLLFLAAVAAATLSCNGQAHGSDICDKADYVRLCRFAVKGSNDPRVALKN 60

Query: 61  SIEYLISETKKAVAMSEKLGKTQHLTVCKENLNSAIDDLHKSLEYLESGDKNGLKTVLTG 120
           +IE L+S+T+ A A S++LG +Q + VC EN +SAIDDL KSL+YLES DK  L++ LTG
Sbjct: 61  TIEVLMSQTESAKASSQRLGNSQFIAVCTENFDSAIDDLVKSLQYLESNDKASLQSSLTG 120

Query: 121 ASTYYVTCDDAVAEG--GIVQLAADLLAADTKLHRMANNCLHLSSLL 162
           + ++YVTC D + EG  G+++LA++LL  D  L RM  N L+L+SLL
Sbjct: 121 SLSFYVTCKDTLVEGGDGVMKLASNLLIKDDMLQRMGANSLYLASLL 166

[Close]

BLAST of Lag0039973 vs. NCBI nr
Match: XP_008446181.1 (PREDICTED: uncharacterized protein LOC103488985 [Cucumis melo] >KAA0034294.1 pectinesterase inhibitor-like [Cucumis melo var. makuwa] >TYK15625.1 pectinesterase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 153.7 bits (387), Expect = 1.4e-33
Identity = 87/168 (51.79%), Postives = 119/168 (70.83%), Query Frame = 0


Query: 1   MAEKYLVSLISLVFL----AAVAIFAGQAQGADLCAKAEFVPLCQSVVKGATDPKAALKT 60
           MA+K  VS  S VFL    A V +FAGQAQG D+CA+AE+VPLC+SVVKGA+DP  A+KT
Sbjct: 1   MAKKQSVS-FSPVFLVVATAVVVLFAGQAQGVDICAQAEYVPLCRSVVKGASDPTVAIKT 60

Query: 61  SIEYLISETKKAVAMSEKLGKTQHLTVCKENLNSAIDDLHKSLEYLESGDKNGLKTVLTG 120
           +I++L  ETK+A   S  LG  Q +  C +N +SA+D+L KSLEYL+  D   L+ +L+G
Sbjct: 61  AIQHLSFETKRAKTASSILGNKQAIDACTQNYDSALDNLQKSLEYLQIKDLPSLRVMLSG 120

Query: 121 ASTYYVTCDDAVAE---GGIVQLAADLLAADTKLHRMANNCLHLSSLL 162
           A ++YV+C DAVAE    G+V++A ++   DT L  +A NCLH++SLL
Sbjct: 121 ALSFYVSCTDAVAEVSTFGVVKMAKNVEKTDTALQHLAGNCLHIASLL 167

The following BLAST results are available for this feature:

BLAST of Lag0039973 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891721.1	1.1e-35	53.01	uncharacterized protein LOC120081119 [Benincasa hispida]	[more]
KAG7032299.1	2.8e-34	55.90	hypothetical protein SDJN02_06343, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022957327.1	3.6e-34	54.66	pectinesterase 3-like [Cucurbita moschata]	[more]
XP_022983388.1	4.8e-34	53.29	uncharacterized protein LOC111481993 [Cucurbita maxima]	[more]
XP_008446181.1	1.4e-33	51.79	PREDICTED: uncharacterized protein LOC103488985 [Cucumis melo] >KAA0034294.1 pec...	[more]

BLAST of Lag0039973 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 22..157 e-value: 2.4E-19 score: 80.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 29..156 e-value: 1.4E-15 score: 57.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 6..160 e-value: 3.3E-14 score: 50.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 28..162 e-value: 1.0E-18 score: 69.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 29..161
None	No IPR available	PANTHER	PTHR31080:SF245	INVERTASE INHIBITOR-RELATED	coord: 11..157
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 11..157
None	No IPR available	CDD	cd15800	PMEI-like_2	coord: 24..161 e-value: 1.12726E-33 score: 114.379

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0039973.1	Lag0039973.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004857	enzyme inhibitor activity