Lag0039868 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0039868
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	urease
Location	chr13: 528278 .. 530048 (+)
RNA-Seq Expression	Lag0039868
Synteny	Lag0039868

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTAATTTGGTTAGCTATGCTCTGATGTGATCGGGACATCTCTACTTCTTCTAAGAGGTCGTAGGTTCGATTTCCCTCCCTCATATTTTTACATCTTGTCCTTGTATTTGTTTGAGCAGTGAAGGAGCTGGAGGCGGTCATGCTCCAGATATTATCCGAGTATGCAGTCTGAAAAATGTGCTGCCTTCATCAACTAATCCAACACGACCGTTCACATTGAATACTATAGATGAACATCTTGACATGCTGGTATAAAAACTAATTAAAGTTAGTTCACAATTCCCATTCAACTAACCTGAGTTTCCCAATACAGTTTCTATTTGCTGACATACTTTGTGCATTGAAGAACTGTAAAAGAAAAATGGAAGAAAAAAAGATTTGAGTTTGGCTTTTATTCCATACAGATGGTGTGCCATCATCTTGATCCCAATATTGAAGAAGATGTAGCTTTTGCCGAATCAAGGATAAGGAAGGAAACTATTGCTGCAGAAGATATTTTGCATGATTTGGGAGCAATTAGTATGATATCTTCTGATTCACAGGCCATGGGCCGCATCGGAGAGGTTCTTAGTGCATTTTCATCCTCTTTTTTTCTCCATTTTATTTCATAAGTGCAATCGAAGGTAGTTTTTTTTCCCCCCATTTTTAACATTGTTGTCCTCTGTTTCTCATCAATCCTACTGTATTTTCTTTAGGACAATGGAGAGCACACATCTCGTGATCGCAAATGATTCATGTTTTAATTAATGATTTGTATCATATGGGTTGTTTTGATCCACAATTTCGTTCGTTTATGTATCGTTTGCTCAGGTGATTAGCAGAACATGGCAAACTGCACACAAAATGAAGTTGCAAAGAGGATCGCTTGATTACGGGAAACCAGAAAATGACAACCTTCGGATCAAGCGATATATCTCCAAATATACCATAAATCCAGCCATAGCAAACGGGTTTTCTCAATATATTGGTTCAGTAGAGGTATTGTAATTTTGATTCTTCAACTTAATTAAATTTTACATGATGAGCTAGACATTACCATTAATATCAAGTCAACAACACCTGAAGATGAAAATTTAATTATATGCAGAAAGTTAAGCATGGCTTGGCTTGAACTTGCAAAGTCTCTGTGTTTGTAAATGCCCCTCCAACTTAAATAAATTCCACATTTCTTTATTTTGTTCTCTGACTCGTGGTTTCTTTATTTTGCACTTTAATAAACACTGATTTAGGTTTATTTCAGATACAAAACAGAGAAAAGGAAGAAGATGAAGGCTCTCAATATCTTTGTATTGCTCACAATGATTATTACAAGATACAAAACAATTGAATAAATAGGCAAGCAGGAATAACAAGAAAGATTCCTTGCCCTATAGAATGCTGCCACGAATATACACAGAAAGAATAAAATAGCAGAAAGGATAAAAGGTAAAATAACCCCAGCACTCTACATTTTACCATCAGGTAATTCTTCCAAGGAAGCACCATAGACCTGTGCTCTAATAATTTCCATATTGAATCTTTTTATTGACAAGGTTTCGCTGATTCCTCGAGTTTTAACATCCTACGTGGTAATTAAATTTGTGTTAATGATCTTATAATTTATCCTCTCCGTCCCACAGGTAGGGAAGTTGGCTGATCTTGTTCTGTGGAAGCCATCATTTTTTGGGGCAAAACCAGAAAAGGTGATTAAAGATGGTACAATTGCATGGGCCAACATGGGTGATCCAAATGCAAGCATTCCCACACCTGAACCGGTAAAATTCTATTAG

mRNA sequence

ATGTTAATTTGTGAAGGAGCTGGAGGCGGTCATGCTCCAGATATTATCCGAGTATGCAGTCTGAAAAATGTGCTGCCTTCATCAACTAATCCAACACGACCGTTCACATTGAATACTATAGATGAACATCTTGACATGCTGATGGTGTGCCATCATCTTGATCCCAATATTGAAGAAGATGTAGCTTTTGCCGAATCAAGGATAAGGAAGGAAACTATTGCTGCAGAAGATATTTTGCATGATTTGGGAGCAATTAGTATGATATCTTCTGATTCACAGGCCATGGGCCGCATCGGAGAGGTGATTAGCAGAACATGGCAAACTGCACACAAAATGAAGTTGCAAAGAGGATCGCTTGATTACGGGAAACCAGAAAATGACAACCTTCGGATCAAGCGATATATCTCCAAATATACCATAAATCCAGCCATAGCAAACGGGTTTTCTCAATATATTGGTTCAGTAGAGGTAGGGAAGTTGGCTGATCTTGTTCTGTGGAAGCCATCATTTTTTGGGGCAAAACCAGAAAAGGTGATTAAAGATGGTACAATTGCATGGGCCAACATGGGTGATCCAAATGCAAGCATTCCCACACCTGAACCGGTAAAATTCTATTAG

Coding sequence (CDS)

ATGTTAATTTGTGAAGGAGCTGGAGGCGGTCATGCTCCAGATATTATCCGAGTATGCAGTCTGAAAAATGTGCTGCCTTCATCAACTAATCCAACACGACCGTTCACATTGAATACTATAGATGAACATCTTGACATGCTGATGGTGTGCCATCATCTTGATCCCAATATTGAAGAAGATGTAGCTTTTGCCGAATCAAGGATAAGGAAGGAAACTATTGCTGCAGAAGATATTTTGCATGATTTGGGAGCAATTAGTATGATATCTTCTGATTCACAGGCCATGGGCCGCATCGGAGAGGTGATTAGCAGAACATGGCAAACTGCACACAAAATGAAGTTGCAAAGAGGATCGCTTGATTACGGGAAACCAGAAAATGACAACCTTCGGATCAAGCGATATATCTCCAAATATACCATAAATCCAGCCATAGCAAACGGGTTTTCTCAATATATTGGTTCAGTAGAGGTAGGGAAGTTGGCTGATCTTGTTCTGTGGAAGCCATCATTTTTTGGGGCAAAACCAGAAAAGGTGATTAAAGATGGTACAATTGCATGGGCCAACATGGGTGATCCAAATGCAAGCATTCCCACACCTGAACCGGTAAAATTCTATTAG

Protein sequence

MLICEGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFAESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKPENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTIAWANMGDPNASIPTPEPVKFY

Homology

[Close]

BLAST of Lag0039868 vs. NCBI nr
Match: KAG7012742.1 (Urease, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 378.6 bits (971), Expect = 3.3e-101
Identity = 182/198 (91.92%), Postives = 191/198 (96.46%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDIIRVCS+KNVLPSSTNPTRPFT NTIDEHLDMLMVCHHLD NI+ED+AFA
Sbjct: 640 EGAGGGHAPDIIRVCSVKNVLPSSTNPTRPFTSNTIDEHLDMLMVCHHLDRNIKEDLAFA 699

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKP 124
           ESRIR+ETIAAEDILHD+GAIS+ISSDSQAMGRIGEVI RTWQTAHKMKLQRG LD  KP
Sbjct: 700 ESRIREETIAAEDILHDMGAISIISSDSQAMGRIGEVICRTWQTAHKMKLQRGPLDSSKP 759

Query: 125 ENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTI 184
           +NDNLRIKRY+SKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIK GTI
Sbjct: 760 DNDNLRIKRYVSKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKGGTI 819

Query: 185 AWANMGDPNASIPTPEPV 203
           AWANMGDPNASIPTPEP+
Sbjct: 820 AWANMGDPNASIPTPEPI 837

[Close]

BLAST of Lag0039868 vs. NCBI nr
Match: KAG6573656.1 (hypothetical protein SDJN03_27543, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 378.6 bits (971), Expect = 3.3e-101
Identity = 182/198 (91.92%), Postives = 191/198 (96.46%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDIIRVCS+KNVLPSSTNPTRPFT NTIDEHLDMLMVCHHLD NI+ED+AFA
Sbjct: 523 EGAGGGHAPDIIRVCSVKNVLPSSTNPTRPFTSNTIDEHLDMLMVCHHLDRNIKEDLAFA 582

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKP 124
           ESRIR+ETIAAEDILHD+GAIS+ISSDSQAMGRIGEVI RTWQTAHKMKLQRG LD  KP
Sbjct: 583 ESRIREETIAAEDILHDMGAISIISSDSQAMGRIGEVICRTWQTAHKMKLQRGPLDSSKP 642

Query: 125 ENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTI 184
           +NDNLRIKRY+SKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIK GTI
Sbjct: 643 DNDNLRIKRYVSKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKGGTI 702

Query: 185 AWANMGDPNASIPTPEPV 203
           AWANMGDPNASIPTPEP+
Sbjct: 703 AWANMGDPNASIPTPEPI 720

[Close]

BLAST of Lag0039868 vs. NCBI nr
Match: XP_022945701.1 (urease-like [Cucurbita moschata])

HSP 1 Score: 378.3 bits (970), Expect = 4.3e-101
Identity = 181/198 (91.41%), Postives = 191/198 (96.46%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDIIRVCS+KNVLPSSTNPTRPFT NTIDEHLDMLMVCHHLD NI+ED+AFA
Sbjct: 545 EGAGGGHAPDIIRVCSVKNVLPSSTNPTRPFTSNTIDEHLDMLMVCHHLDRNIKEDLAFA 604

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKP 124
           ESRIR+ETIAAEDILHD+GAIS+ISSDSQAMGRIGEV+ RTWQTAHKMKLQRG LD  KP
Sbjct: 605 ESRIREETIAAEDILHDMGAISIISSDSQAMGRIGEVVCRTWQTAHKMKLQRGPLDSSKP 664

Query: 125 ENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTI 184
           +NDNLRIKRY+SKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIK GTI
Sbjct: 665 DNDNLRIKRYVSKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKGGTI 724

Query: 185 AWANMGDPNASIPTPEPV 203
           AWANMGDPNASIPTPEP+
Sbjct: 725 AWANMGDPNASIPTPEPI 742

[Close]

BLAST of Lag0039868 vs. NCBI nr
Match: XP_023541540.1 (LOW QUALITY PROTEIN: urease-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 377.9 bits (969), Expect = 5.7e-101
Identity = 182/198 (91.92%), Postives = 191/198 (96.46%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDIIRVCS+KNVLPSSTNPTRPFT NTIDEHLDMLMVCHHLD NI+ED+AFA
Sbjct: 544 EGAGGGHAPDIIRVCSVKNVLPSSTNPTRPFTSNTIDEHLDMLMVCHHLDRNIKEDLAFA 603

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKP 124
           ESRIR+ETIAAEDILHD+GAIS+ISSDSQAMGRIGEVI RTWQTAHKMKLQRG LD  KP
Sbjct: 604 ESRIREETIAAEDILHDMGAISIISSDSQAMGRIGEVICRTWQTAHKMKLQRGPLDSKKP 663

Query: 125 ENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTI 184
           +NDNLRIKRY+SKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIK GTI
Sbjct: 664 DNDNLRIKRYVSKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKGGTI 723

Query: 185 AWANMGDPNASIPTPEPV 203
           AWANMGDPNASIPTPEP+
Sbjct: 724 AWANMGDPNASIPTPEPI 741

[Close]

BLAST of Lag0039868 vs. NCBI nr
Match: KAE8023675.1 (hypothetical protein FH972_009347 [Carpinus fangiana])

HSP 1 Score: 370.2 bits (949), Expect = 1.2e-98
Identity = 178/200 (89.00%), Postives = 187/200 (93.50%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDII+VC +KNVLPSSTNPTRPFT NTIDEHLDMLMVCHHLD NI EDV+FA
Sbjct: 554 EGAGGGHAPDIIKVCGVKNVLPSSTNPTRPFTSNTIDEHLDMLMVCHHLDKNIPEDVSFA 613

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKP 124
           ESRIR ETIAAEDILHDLGAIS+ISSDSQAMGRIGEVISRTWQTAHKMKLQRGS+D   P
Sbjct: 614 ESRIRAETIAAEDILHDLGAISIISSDSQAMGRIGEVISRTWQTAHKMKLQRGSIDRSGP 673

Query: 125 ENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTI 184
           +NDNLRIKRYI+KYTINPAIA GFSQY+GSVEVGKLADLV+WKPSFFGAKPE VIK G I
Sbjct: 674 DNDNLRIKRYIAKYTINPAIATGFSQYVGSVEVGKLADLVIWKPSFFGAKPEMVIKGGAI 733

Query: 185 AWANMGDPNASIPTPEPVKF 205
           AWANMGDPNASIP PEPVKF
Sbjct: 734 AWANMGDPNASIPRPEPVKF 753

[Close]

BLAST of Lag0039868 vs. ExPASy Swiss-Prot
Match: Q9SR52 (Urease OS=Arabidopsis thaliana OX=3702 GN=URE PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 7.7e-93
Identity = 164/198 (82.83%), Postives = 179/198 (90.40%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDIIRVC +KNVLPSSTNPTRP+T NT+DEHLDMLMVCHHLD NI EDVAFA
Sbjct: 545 EGAGGGHAPDIIRVCGVKNVLPSSTNPTRPYTKNTVDEHLDMLMVCHHLDKNIPEDVAFA 604

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKP 124
           ESRIR ETIAAEDILHD+GAIS+ISSDSQAMGRIGEVISRTWQTA KMK QRG++D    
Sbjct: 605 ESRIRAETIAAEDILHDMGAISIISSDSQAMGRIGEVISRTWQTADKMKAQRGAIDPNMA 664

Query: 125 ENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTI 184
           ++DN RIKRYI+KYTINPAIANGF+  IGSVEV KLADLV+W+P+FFGAKPE +IK G I
Sbjct: 665 DDDNSRIKRYIAKYTINPAIANGFADLIGSVEVKKLADLVIWQPAFFGAKPEMIIKGGNI 724

Query: 185 AWANMGDPNASIPTPEPV 203
           AWANMGD NASIPTPEPV
Sbjct: 725 AWANMGDANASIPTPEPV 742

[Close]

BLAST of Lag0039868 vs. ExPASy Swiss-Prot
Match: E0ZS48 (Urease OS=Oryza sativa subsp. indica OX=39946 PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 7.7e-93
Identity = 167/203 (82.27%), Postives = 180/203 (88.67%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDII+VC +KNVLPSSTNPTRPFTLNT+DEHLDMLMVCHHLD NI EDVAFA
Sbjct: 545 EGAGGGHAPDIIKVCGVKNVLPSSTNPTRPFTLNTVDEHLDMLMVCHHLDRNIPEDVAFA 604

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGK- 124
           ESRIR ETIAAEDILHD+GAIS+ISSDSQAMGRIGEVI+RTWQTA+KMK QRG L     
Sbjct: 605 ESRIRAETIAAEDILHDMGAISIISSDSQAMGRIGEVITRTWQTANKMKRQRGRLPISSS 664

Query: 125 ----PENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVI 184
                +NDN RI+RYI+KYTINPAI NGFS ++GSVEVGKLADLV+WKPSFFGAKPE VI
Sbjct: 665 PDAAEDNDNFRIRRYIAKYTINPAIVNGFSDFVGSVEVGKLADLVIWKPSFFGAKPEMVI 724

Query: 185 KDGTIAWANMGDPNASIPTPEPV 203
           K G IA ANMGDPNASIPTPEPV
Sbjct: 725 KGGAIACANMGDPNASIPTPEPV 747

[Close]

BLAST of Lag0039868 vs. ExPASy Swiss-Prot
Match: B9GCH9 (Urease OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0234800 PE=3 SV=2)

HSP 1 Score: 341.3 bits (874), Expect = 7.7e-93
Identity = 167/203 (82.27%), Postives = 180/203 (88.67%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDII+VC +KNVLPSSTNPTRPFTLNT+DEHLDMLMVCHHLD NI EDVAFA
Sbjct: 545 EGAGGGHAPDIIKVCGVKNVLPSSTNPTRPFTLNTVDEHLDMLMVCHHLDRNIPEDVAFA 604

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGK- 124
           ESRIR ETIAAEDILHD+GAIS+ISSDSQAMGRIGEVI+RTWQTA+KMK QRG L     
Sbjct: 605 ESRIRAETIAAEDILHDMGAISIISSDSQAMGRIGEVITRTWQTANKMKRQRGRLPISSS 664

Query: 125 ----PENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVI 184
                +NDN RI+RYI+KYTINPAI NGFS ++GSVEVGKLADLV+WKPSFFGAKPE VI
Sbjct: 665 PDAAEDNDNFRIRRYIAKYTINPAIVNGFSDFVGSVEVGKLADLVIWKPSFFGAKPEMVI 724

Query: 185 KDGTIAWANMGDPNASIPTPEPV 203
           K G IA ANMGDPNASIPTPEPV
Sbjct: 725 KGGAIACANMGDPNASIPTPEPV 747

[Close]

BLAST of Lag0039868 vs. ExPASy Swiss-Prot
Match: P07374 (Urease OS=Canavalia ensiformis OX=3823 PE=1 SV=3)

HSP 1 Score: 339.7 bits (870), Expect = 2.2e-92
Identity = 161/199 (80.90%), Postives = 178/199 (89.45%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDII+VC +KNVLPSSTNPTRP T NTIDEHLDMLMVCHHLD  I ED+AFA
Sbjct: 547 EGAGGGHAPDIIKVCGIKNVLPSSTNPTRPLTSNTIDEHLDMLMVCHHLDREIPEDLAFA 606

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKP 124
            SRIRK+TIAAED+L+D+GAIS+ISSDSQAMGR+GEVISRTWQTA KMK Q G L     
Sbjct: 607 HSRIRKKTIAAEDVLNDIGAISIISSDSQAMGRVGEVISRTWQTADKMKAQTGPLKCDSS 666

Query: 125 ENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTI 184
           +NDN RI+RYI+KYTINPAIANGFSQY+GSVEVGKLADLV+WKPSFFG KPE VIK G +
Sbjct: 667 DNDNFRIRRYIAKYTINPAIANGFSQYVGSVEVGKLADLVMWKPSFFGTKPEMVIKGGMV 726

Query: 185 AWANMGDPNASIPTPEPVK 204
           AWA++GDPNASIPTPEPVK
Sbjct: 727 AWADIGDPNASIPTPEPVK 745

[Close]

BLAST of Lag0039868 vs. ExPASy Swiss-Prot
Match: Q8YQZ0 (Urease subunit alpha OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=ureC PE=3 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 3.0e-89
Identity = 157/198 (79.29%), Postives = 175/198 (88.38%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDII+VC   NVLPSSTNPTRP+T+NT+DEHLDMLMVCHHLDP I EDVAFA
Sbjct: 276 EGAGGGHAPDIIKVCGQANVLPSSTNPTRPYTVNTLDEHLDMLMVCHHLDPAIAEDVAFA 335

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKP 124
           ESRIR+ETIAAEDILHDLGA SMI+SDSQAMGR+GEVI RTWQT+HKMK+QRGSL  G  
Sbjct: 336 ESRIRRETIAAEDILHDLGAFSMIASDSQAMGRVGEVIIRTWQTSHKMKVQRGSLT-GDA 395

Query: 125 ENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTI 184
           E DNLR KRY++KYTINPAI +G +QY+GSVE GKLADL LW+P+FFG KPE VIK G I
Sbjct: 396 EADNLRAKRYVAKYTINPAITHGIAQYVGSVEAGKLADLCLWRPAFFGVKPEIVIKGGMI 455

Query: 185 AWANMGDPNASIPTPEPV 203
           AW+ MGD NASIPTP+PV
Sbjct: 456 AWSQMGDANASIPTPQPV 472

[Close]

BLAST of Lag0039868 vs. ExPASy TrEMBL
Match: A0A6J1G1M4 (Urease OS=Cucurbita moschata OX=3662 GN=LOC111449851 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.1e-101
Identity = 181/198 (91.41%), Postives = 191/198 (96.46%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDIIRVCS+KNVLPSSTNPTRPFT NTIDEHLDMLMVCHHLD NI+ED+AFA
Sbjct: 545 EGAGGGHAPDIIRVCSVKNVLPSSTNPTRPFTSNTIDEHLDMLMVCHHLDRNIKEDLAFA 604

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKP 124
           ESRIR+ETIAAEDILHD+GAIS+ISSDSQAMGRIGEV+ RTWQTAHKMKLQRG LD  KP
Sbjct: 605 ESRIREETIAAEDILHDMGAISIISSDSQAMGRIGEVVCRTWQTAHKMKLQRGPLDSSKP 664

Query: 125 ENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTI 184
           +NDNLRIKRY+SKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIK GTI
Sbjct: 665 DNDNLRIKRYVSKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKGGTI 724

Query: 185 AWANMGDPNASIPTPEPV 203
           AWANMGDPNASIPTPEP+
Sbjct: 725 AWANMGDPNASIPTPEPI 742

[Close]

BLAST of Lag0039868 vs. ExPASy TrEMBL
Match: A0A5N6R4J3 (Urea amidohydrolase OS=Carpinus fangiana OX=176857 GN=FH972_009347 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 5.7e-99
Identity = 178/200 (89.00%), Postives = 187/200 (93.50%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDII+VC +KNVLPSSTNPTRPFT NTIDEHLDMLMVCHHLD NI EDV+FA
Sbjct: 554 EGAGGGHAPDIIKVCGVKNVLPSSTNPTRPFTSNTIDEHLDMLMVCHHLDKNIPEDVSFA 613

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKP 124
           ESRIR ETIAAEDILHDLGAIS+ISSDSQAMGRIGEVISRTWQTAHKMKLQRGS+D   P
Sbjct: 614 ESRIRAETIAAEDILHDLGAISIISSDSQAMGRIGEVISRTWQTAHKMKLQRGSIDRSGP 673

Query: 125 ENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTI 184
           +NDNLRIKRYI+KYTINPAIA GFSQY+GSVEVGKLADLV+WKPSFFGAKPE VIK G I
Sbjct: 674 DNDNLRIKRYIAKYTINPAIATGFSQYVGSVEVGKLADLVIWKPSFFGAKPEMVIKGGAI 733

Query: 185 AWANMGDPNASIPTPEPVKF 205
           AWANMGDPNASIP PEPVKF
Sbjct: 734 AWANMGDPNASIPRPEPVKF 753

[Close]

BLAST of Lag0039868 vs. ExPASy TrEMBL
Match: A0A5N6R1H9 (Urease OS=Carpinus fangiana OX=176857 GN=FH972_009321 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.2e-98
Identity = 177/198 (89.39%), Postives = 187/198 (94.44%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDII+VC +KNVLPSSTNPTRPFT NTIDEHLDMLMVCHHLD NI EDV+FA
Sbjct: 574 EGAGGGHAPDIIKVCGVKNVLPSSTNPTRPFTSNTIDEHLDMLMVCHHLDKNIPEDVSFA 633

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKP 124
           ESRIR ETIAAEDILHD+GAIS+ISSDSQAMGRIGEVISRTWQTAHKMKLQRGS+D   P
Sbjct: 634 ESRIRAETIAAEDILHDMGAISIISSDSQAMGRIGEVISRTWQTAHKMKLQRGSIDPSGP 693

Query: 125 ENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTI 184
           +NDNLRIKRYI+KYTINPAIANGFSQY+GSVEVGKLADLV+WKPSFFGAKPE VIK G I
Sbjct: 694 DNDNLRIKRYIAKYTINPAIANGFSQYVGSVEVGKLADLVIWKPSFFGAKPEIVIKGGAI 753

Query: 185 AWANMGDPNASIPTPEPV 203
           AWANMGDPNASIPTPEPV
Sbjct: 754 AWANMGDPNASIPTPEPV 771

[Close]

BLAST of Lag0039868 vs. ExPASy TrEMBL
Match: A0A438DIV8 (Urea amidohydrolase OS=Vitis vinifera OX=29760 GN=UREA PE=3 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 9.2e-97
Identity = 173/202 (85.64%), Postives = 188/202 (93.07%), Query Frame = 0


Query: 1   MLICEGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEED 60
           +L+CEGAGGGHAPDII+VC +KNVLPSSTNPTRPFT NTIDEHLDMLMVCHHLD +I ED
Sbjct: 51  VLLCEGAGGGHAPDIIKVCGVKNVLPSSTNPTRPFTSNTIDEHLDMLMVCHHLDKDIPED 110

Query: 61  VAFAESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLD 120
           VAFAESRIR ETIAAEDILHD+GAIS+I+SDSQAMGRIGEVI RTWQTAHKMKLQRGSLD
Sbjct: 111 VAFAESRIRAETIAAEDILHDMGAISIIASDSQAMGRIGEVIIRTWQTAHKMKLQRGSLD 170

Query: 121 YGKPENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIK 180
               +NDNLRIKRYI+KYTINPAIANGFS+++GS+EVGK+ADLVLW PSFFGAKPE VIK
Sbjct: 171 ASGVDNDNLRIKRYIAKYTINPAIANGFSRFVGSIEVGKVADLVLWNPSFFGAKPEMVIK 230

Query: 181 DGTIAWANMGDPNASIPTPEPV 203
            G IAWANMGDPNASIPTPEPV
Sbjct: 231 GGVIAWANMGDPNASIPTPEPV 252

[Close]

BLAST of Lag0039868 vs. ExPASy TrEMBL
Match: A0A5J5ASI6 (Urease OS=Nyssa sinensis OX=561372 GN=F0562_033188 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 2.0e-96
Identity = 175/198 (88.38%), Postives = 185/198 (93.43%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDII+VC +KNVLPSSTNPTRPFT NTIDEHLDMLMVCHHLD +I EDVAFA
Sbjct: 544 EGAGGGHAPDIIKVCGVKNVLPSSTNPTRPFTSNTIDEHLDMLMVCHHLDKDIPEDVAFA 603

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKP 124
           ESRIR ETIAAEDILHD+GAIS+I+SDSQAMGRIGEVISRTWQTAHKMKL RGS+  G  
Sbjct: 604 ESRIRAETIAAEDILHDMGAISIIASDSQAMGRIGEVISRTWQTAHKMKLLRGSVGNGGS 663

Query: 125 ENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTI 184
           ENDNLRIKRYI+KYTINPAIANGFSQY+GSVEVGKLADLV+WKPSFFGAKPE VIK G I
Sbjct: 664 ENDNLRIKRYIAKYTINPAIANGFSQYVGSVEVGKLADLVIWKPSFFGAKPEMVIKGGDI 723

Query: 185 AWANMGDPNASIPTPEPV 203
           AWANMGDPNASIPTPEPV
Sbjct: 724 AWANMGDPNASIPTPEPV 741

[Close]

BLAST of Lag0039868 vs. TAIR 10
Match: AT1G67550.1 (urease )

HSP 1 Score: 341.3 bits (874), Expect = 5.5e-94
Identity = 164/198 (82.83%), Postives = 179/198 (90.40%), Query Frame = 0


Query: 5   EGAGGGHAPDIIRVCSLKNVLPSSTNPTRPFTLNTIDEHLDMLMVCHHLDPNIEEDVAFA 64
           EGAGGGHAPDIIRVC +KNVLPSSTNPTRP+T NT+DEHLDMLMVCHHLD NI EDVAFA
Sbjct: 545 EGAGGGHAPDIIRVCGVKNVLPSSTNPTRPYTKNTVDEHLDMLMVCHHLDKNIPEDVAFA 604

Query: 65  ESRIRKETIAAEDILHDLGAISMISSDSQAMGRIGEVISRTWQTAHKMKLQRGSLDYGKP 124
           ESRIR ETIAAEDILHD+GAIS+ISSDSQAMGRIGEVISRTWQTA KMK QRG++D    
Sbjct: 605 ESRIRAETIAAEDILHDMGAISIISSDSQAMGRIGEVISRTWQTADKMKAQRGAIDPNMA 664

Query: 125 ENDNLRIKRYISKYTINPAIANGFSQYIGSVEVGKLADLVLWKPSFFGAKPEKVIKDGTI 184
           ++DN RIKRYI+KYTINPAIANGF+  IGSVEV KLADLV+W+P+FFGAKPE +IK G I
Sbjct: 665 DDDNSRIKRYIAKYTINPAIANGFADLIGSVEVKKLADLVIWQPAFFGAKPEMIIKGGNI 724

Query: 185 AWANMGDPNASIPTPEPV 203
           AWANMGD NASIPTPEPV
Sbjct: 725 AWANMGDANASIPTPEPV 742

The following BLAST results are available for this feature:

BLAST of Lag0039868 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012742.1	3.3e-101	91.92	Urease, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6573656.1	3.3e-101	91.92	hypothetical protein SDJN03_27543, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022945701.1	4.3e-101	91.41	urease-like [Cucurbita moschata]	[more]
XP_023541540.1	5.7e-101	91.92	LOW QUALITY PROTEIN: urease-like [Cucurbita pepo subsp. pepo]	[more]
KAE8023675.1	1.2e-98	89.00	hypothetical protein FH972_009347 [Carpinus fangiana]	[more]

BLAST of Lag0039868 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SR52	7.7e-93	82.83	Urease OS=Arabidopsis thaliana OX=3702 GN=URE PE=3 SV=1	[more]
E0ZS48	7.7e-93	82.27	Urease OS=Oryza sativa subsp. indica OX=39946 PE=1 SV=1	[more]
B9GCH9	7.7e-93	82.27	Urease OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0234800 PE=3 SV=2	[more]
P07374	2.2e-92	80.90	Urease OS=Canavalia ensiformis OX=3823 PE=1 SV=3	[more]
Q8YQZ0	3.0e-89	79.29	Urease subunit alpha OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=...	[more]

BLAST of Lag0039868 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G1M4	2.1e-101	91.41	Urease OS=Cucurbita moschata OX=3662 GN=LOC111449851 PE=3 SV=1	[more]
A0A5N6R4J3	5.7e-99	89.00	Urea amidohydrolase OS=Carpinus fangiana OX=176857 GN=FH972_009347 PE=3 SV=1	[more]
A0A5N6R1H9	2.2e-98	89.39	Urease OS=Carpinus fangiana OX=176857 GN=FH972_009321 PE=3 SV=1	[more]
A0A438DIV8	9.2e-97	85.64	Urea amidohydrolase OS=Vitis vinifera OX=29760 GN=UREA PE=3 SV=1	[more]
A0A5J5ASI6	2.0e-96	88.38	Urease OS=Nyssa sinensis OX=561372 GN=F0562_033188 PE=3 SV=1	[more]

BLAST of Lag0039868 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G67550.1	5.5e-94	82.83	urease	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005848	Urease, alpha subunit	PRINTS	PR01752	UREASE	coord: 158..171 score: 78.45 coord: 65..81 score: 85.82 coord: 132..147 score: 80.74 coord: 26..43 score: 76.59
IPR006680	Amidohydrolase-related	PFAM	PF01979	Amidohydro_1	coord: 3..182 e-value: 1.2E-39 score: 136.7
None	No IPR available	GENE3D	3.20.20.140		coord: 1..180 e-value: 1.2E-91 score: 309.3
None	No IPR available	PANTHER	PTHR43440	UREASE	coord: 5..204
IPR017950	Urease active site	PROSITE	PS00145	UREASE_2	coord: 48..64
IPR017951	Urease alpha subunit, C-terminal	PROSITE	PS51368	UREASE_3	coord: 1..205 score: 110.5149
IPR032466	Metal-dependent hydrolase	SUPERFAMILY	51556	Metallo-dependent hydrolases	coord: 4..164
IPR011059	Metal-dependent hydrolase, composite domain superfamily	SUPERFAMILY	51338	Composite domain of metallo-dependent hydrolases	coord: 153..202

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0039868.1	Lag0039868.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043419	urea catabolic process
biological_process	GO:0006807	nitrogen compound metabolic process
cellular_component	GO:0035550	urease complex
molecular_function	GO:0016151	nickel cation binding
molecular_function	GO:0009039	urease activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds