Lag0039838 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0039838 vs. NCBI nr
Match: XP_022945246.1 (4-coumarate--CoA ligase-like 1 [Cucurbita moschata])

HSP 1 Score: 1042.7 bits (2695), Expect = 1.1e-300
Identity = 530/559 (94.81%), Postives = 550/559 (98.39%), Query Frame = 0

Query: 1   MPAANNNPDMATCIRDSVEDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEA 60
           M AANNN  MATCIRD VEDEEHIFRSQLPEV VPDDITLPEFVLQNAESYA+NVAFVEA
Sbjct: 48  MSAANNNSAMATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEA 107

Query: 61  VSGKAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGV 120
           VSGKAYTYREVVRDT+RFAKALSSLRL+KGQVV+VVLPNVAEYAIVALGIMAAGGVFSGV
Sbjct: 108 VSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGV 167

Query: 121 NPAAHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEGSMNWHKLLEAAD 180
           NPAAHISEIKKQVEVADAKLVVTNS++FEKVKELKLPVIV+GEELIEGSMNWHKLLEAAD
Sbjct: 168 NPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAAD 227

Query: 181 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTT 240
           RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM GKVTT
Sbjct: 228 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTT 287

Query: 241 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 300
           LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV
Sbjct: 288 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 347

Query: 301 KNPIVEEFDLSALKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGS 360
           KNPIV+EFDLS LKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYGS
Sbjct: 348 KNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGS 407

Query: 361 IGKENLTARKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETS 420
           IGK+NL A+KNTVGRILPNLEVKFIDPDSGRSLPKNTPGE+CVRSQCVMQGYY NEEETS
Sbjct: 408 IGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETS 467

Query: 421 RTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 480
           RTID+KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV
Sbjct: 468 RTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 527

Query: 481 VPLPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKV 540
           VPLPD+EAGEIPAAS+V+APN+KETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKV
Sbjct: 528 VPLPDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKV 587

Query: 541 MRRLIKEKMIEKIRAESSS 560
           MRRLIKEKMIEKIRAE++S
Sbjct: 588 MRRLIKEKMIEKIRAEAAS 606

BLAST of Lag0039838 vs. NCBI nr
Match: KAG7012722.1 (4-coumarate--CoA ligase-like 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1042.0 bits (2693), Expect = 1.9e-300
Identity = 529/559 (94.63%), Postives = 550/559 (98.39%), Query Frame = 0

Query: 1   MPAANNNPDMATCIRDSVEDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEA 60
           M AANNN  MATCIRDSVEDEEHIFRSQLPEV VPDDITLPEFVLQNAESYA+NVAFVEA
Sbjct: 48  MSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEA 107

Query: 61  VSGKAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGV 120
           VSGKAYTYREVVRDT+RFAKALSSLRL+KGQVV+VVLPNVAEYAIVALGIMAAGGVFSGV
Sbjct: 108 VSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGV 167

Query: 121 NPAAHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEGSMNWHKLLEAAD 180
           NPAAHISEIKKQVEVADAKLVVTNS++FEKVKELKLPVIV+GEELIEGSMNWH+LLEAAD
Sbjct: 168 NPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAAD 227

Query: 181 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTT 240
           RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM GKVTT
Sbjct: 228 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTT 287

Query: 241 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 300
           LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV
Sbjct: 288 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 347

Query: 301 KNPIVEEFDLSALKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGS 360
           KNPIV+EFDLS LKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYGS
Sbjct: 348 KNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGS 407

Query: 361 IGKENLTARKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETS 420
           IGK+NL A+KNTVGRILPNLEVKFIDPDSGRSLPKNTPGE+CVRSQCVMQGYY NEEETS
Sbjct: 408 IGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETS 467

Query: 421 RTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 480
           RTID+KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV
Sbjct: 468 RTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 527

Query: 481 VPLPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKV 540
           VPLPD+EAGEIPAAS+V+ PN+KETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKV
Sbjct: 528 VPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKV 587

Query: 541 MRRLIKEKMIEKIRAESSS 560
           MRRLIKEKMIEKIRAE++S
Sbjct: 588 MRRLIKEKMIEKIRAETAS 606

BLAST of Lag0039838 vs. NCBI nr
Match: XP_022966799.1 (4-coumarate--CoA ligase-like 1 [Cucurbita maxima])

HSP 1 Score: 1042.0 bits (2693), Expect = 1.9e-300
Identity = 527/559 (94.28%), Postives = 550/559 (98.39%), Query Frame = 0

Query: 1   MPAANNNPDMATCIRDSVEDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEA 60
           M AANNN  MATCIRDS EDEEHIFRSQLPEV VPDDITLPEFVLQNAE YA+NVAFVEA
Sbjct: 48  MSAANNNSTMATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVAFVEA 107

Query: 61  VSGKAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGV 120
           VSGKAYTYREVVRDT+RFAKALSSLRL+KGQ+V+VVLPNVAEYAIVALGIMAAGGVFSGV
Sbjct: 108 VSGKAYTYREVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGV 167

Query: 121 NPAAHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEGSMNWHKLLEAAD 180
           NPAAHISEIKKQVEVADAKLVVTNS++FEKVKELKLPVIV+GEELIEGSMNWHKLLEAAD
Sbjct: 168 NPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAAD 227

Query: 181 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTT 240
           RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSG+PQEM GKVTT
Sbjct: 228 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTT 287

Query: 241 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 300
           LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV
Sbjct: 288 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 347

Query: 301 KNPIVEEFDLSALKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGS 360
           KNPIV+EFDLS+LKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYG+
Sbjct: 348 KNPIVKEFDLSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGN 407

Query: 361 IGKENLTARKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETS 420
           IGK+NL A+KNTVGRILPNLEVKFIDPDSGRSLPKNTPGE+CVRSQCVMQGYY NEEETS
Sbjct: 408 IGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETS 467

Query: 421 RTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 480
           RTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV
Sbjct: 468 RTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 527

Query: 481 VPLPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKV 540
           VPLPD+EAGEIPAAS+V+APN+KETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKV
Sbjct: 528 VPLPDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKV 587

Query: 541 MRRLIKEKMIEKIRAESSS 560
           MRRLIKEKMIEKIRAE++S
Sbjct: 588 MRRLIKEKMIEKIRAETAS 606

BLAST of Lag0039838 vs. NCBI nr
Match: XP_023541569.1 (4-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1040.8 bits (2690), Expect = 4.3e-300
Identity = 529/559 (94.63%), Postives = 549/559 (98.21%), Query Frame = 0

Query: 1   MPAANNNPDMATCIRDSVEDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEA 60
           M AA NN  MATCIRDSVEDEEHIFRSQLPEV VPDDITLPEFVLQNAESYA+NVAFVEA
Sbjct: 48  MSAATNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEA 107

Query: 61  VSGKAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGV 120
           VSG AYTYREVVRDT+RFAKALSSLRL+KGQVV+VVLPNVAEYAIVALGIMAAGGVFSGV
Sbjct: 108 VSGNAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGV 167

Query: 121 NPAAHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEGSMNWHKLLEAAD 180
           NPAAHISEIKKQVEVADAKLVVTNS++FEKVKELKLPVIV+GEELIEGSMNWHKLLEAAD
Sbjct: 168 NPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAAD 227

Query: 181 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTT 240
           RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM GKVTT
Sbjct: 228 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTT 287

Query: 241 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 300
           LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV
Sbjct: 288 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 347

Query: 301 KNPIVEEFDLSALKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGS 360
           KNPIV+EFDLS LKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYGS
Sbjct: 348 KNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGS 407

Query: 361 IGKENLTARKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETS 420
           IGK+NL A+KNTVGRILPNLEVKFIDPDSGRSLPKNTPGE+CVRSQCVMQGYY NEEETS
Sbjct: 408 IGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETS 467

Query: 421 RTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 480
           RTID+KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV
Sbjct: 468 RTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 527

Query: 481 VPLPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKV 540
           VPLPD+EAGEIPAAS+V+APN+KETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKV
Sbjct: 528 VPLPDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKV 587

Query: 541 MRRLIKEKMIEKIRAESSS 560
           MRRLIKEKMIEKIRAE++S
Sbjct: 588 MRRLIKEKMIEKIRAETAS 606

BLAST of Lag0039838 vs. NCBI nr
Match: XP_038893074.1 (4-coumarate--CoA ligase-like 1 [Benincasa hispida])

HSP 1 Score: 1030.0 bits (2662), Expect = 7.5e-297
Identity = 524/550 (95.27%), Postives = 541/550 (98.36%), Query Frame = 0

Query: 10  MATCIRDSVEDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 69
           MATC RD VEDEEHIFRSQLPEV VPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR
Sbjct: 1   MATCNRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60

Query: 70  EVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 129
           EVVRDTNRF+KAL SLRL+KGQVV+VVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 61  EVVRDTNRFSKALWSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 130 KKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNNFVKE 189
           KKQVEVA+AKLV+TNSANFEKVKELKLPVI+LGEELIEGSMNWHKLLEAADRAGNN VKE
Sbjct: 121 KKQVEVAEAKLVITNSANFEKVKELKLPVILLGEELIEGSMNWHKLLEAADRAGNNCVKE 180

Query: 190 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGLIPFFHI 249
           DIKQ DLCALPFSSGTTGVSKGVMLTHRNL+ANLCSTLSGVPQEMEGKVTTLGLIPFFHI
Sbjct: 181 DIKQNDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 250 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 309
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD
Sbjct: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300

Query: 310 LSALKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAR 369
           LS LKLQAIMTAAAPLAPELQ+AFE+KFPGVDVQEAYGLTEHSCITLNYGSIGKENL A+
Sbjct: 301 LSGLKLQAIMTAAAPLAPELQSAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLAAK 360

Query: 370 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWM 429
           KNTVGRILPNLEVKFIDPDSGRSLPKNTPGE+CVRSQCVMQGYYKNEEETSRTIDNKGWM
Sbjct: 361 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420

Query: 430 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 489
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480

Query: 490 EIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 549
           EIPAASVV+APNSKETE+EIIK+VASNVA+YKKVRLVHFVD IPKSPSGKVMRRL+KEKM
Sbjct: 481 EIPAASVVMAPNSKETEEEIIKHVASNVANYKKVRLVHFVDAIPKSPSGKVMRRLVKEKM 540

Query: 550 IEKIRAESSS 560
           IEKIRAES+S
Sbjct: 541 IEKIRAESAS 550

BLAST of Lag0039838 vs. ExPASy Swiss-Prot
Match: Q9LQ12 (4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 3.5e-228
Identity = 396/532 (74.44%), Postives = 457/532 (85.90%), Query Frame = 0

Query: 19  EDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRF 78
           ED E+IFRS  P V +PD +TLPEFVLQ  E Y ENVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 79  AKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADA 138
           AKAL+SL LRKGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 139 KLVVTNSANFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCA 198
           + ++T++ N+EKVK L LPVIVLGEE IEG++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 199 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCA 258
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  EM G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 259 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSALKLQAI 318
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 319 MTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGRILP 378
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEHSCITL +G   K    A++N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 379 NLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 438
           NLEVKFIDPD+GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 439 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVI 498
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VVI
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 499 APNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMI 551
            P + E E++I+ +VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of Lag0039838 vs. ExPASy Swiss-Prot
Match: Q7XXL2 (4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9 PE=2 SV=2)

HSP 1 Score: 701.8 bits (1810), Expect = 6.1e-201
Identity = 349/539 (64.75%), Postives = 440/539 (81.63%), Query Frame = 0

Query: 19  EDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEAVS-GKAYTYREVVRDTNR 78
           E++EH+FRS+ P V VPD +T+PEFVL  AE+YA+ VA VEA + G++YTY EV RDT R
Sbjct: 14  EEQEHVFRSRFPPVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTAR 73

Query: 79  FAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAD 138
           FA+AL S+ +RKG VV+V LPN+A Y +V+LGIM+AG VFSGVNP A  +EIKKQVE ++
Sbjct: 74  FARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSE 133

Query: 139 AKLVVTNSANFEKVKELKLPVIVLGE-ELIEGSMNWHKLLEAADRAGNNFVKED-IKQTD 198
           AKLVV N   F+KVK+  +PVI +G+ E + G+++W  LL AADR G   V  D  +Q+D
Sbjct: 134 AKLVVANEVAFDKVKDAGVPVIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSD 193

Query: 199 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGI 258
           LCALP+SSGTTGVSKGVML+HRNLV+NLCS++  V  E  G+V TLGL+PFFHIYGITGI
Sbjct: 194 LCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYGITGI 253

Query: 259 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSALKL 318
           CCATLR+KG VVVM RFDLRTF+ AL+   V FAP+VPP++LA+VK+P+ +EFDLS L L
Sbjct: 254 CCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLAL 313

Query: 319 QAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGR 378
           +++MTAAAPLAP+L  AF++KFPGV V+EAYGLTEHSCITL + +       A+K++VG 
Sbjct: 314 KSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGF 373

Query: 379 ILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIG 438
           ILPNLEVKF+DPD+GRSLP NTPGE+CVRSQ VMQGYYK +EET RT+D KGW+HTGD+G
Sbjct: 374 ILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVG 433

Query: 439 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 498
           YID DGDVFIVDRIKELIKYKGFQVAPAELEA+LL+HPS+EDAAV  +PDEEAGE+P A 
Sbjct: 434 YIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVAC 493

Query: 499 VVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIR 555
           VV    ++E E+EI+ YVA  VA YK+VR++H VD IPKS SGK++RR ++++ I++++
Sbjct: 494 VVRRHGAEEGEEEIVAYVAERVASYKRVRVLHIVDAIPKSVSGKILRRQLRDEFIKRMK 552

BLAST of Lag0039838 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.7e-113
Identity = 202/531 (38.04%), Postives = 330/531 (62.15%), Query Frame = 0

Query: 24  IFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAKALS 83
           IFRS+LP++ +P  + L  +  +N   +      ++  + + YTY EV   + + A  L+
Sbjct: 14  IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLN 73

Query: 84  SLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 143
            L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + + AK+V+T
Sbjct: 74  KLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIVIT 133

Query: 144 NSANFEKVKELKLP---VIVLGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALP 203
            +    KVK+  +     ++  + + EG +++ +L+++ +   +      I+  D+ ALP
Sbjct: 134 QACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDE---HEIPDVKIQPDDVVALP 193

Query: 204 FSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGICCA 263
           +SSGTTG+ KGVMLTH+ LV ++   + G      M      + ++P FHIY +  +   
Sbjct: 194 YSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLNSVLLC 253

Query: 264 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSALKLQAI 323
            LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V+ +DLS+++   +
Sbjct: 254 ALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDLSSVR--TV 313

Query: 324 MTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGRILP 383
           M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    +    G ++ 
Sbjct: 314 MSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVR 373

Query: 384 NLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 443
           N E+K +DPD+G SLP+N PGE+C+R   +M+GY  + E T+RTI+ +GW+HTGDIG+ID
Sbjct: 374 NAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIGFID 433

Query: 444 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVI 503
           DD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I DAAVVP+ DE+AGE+P A VV 
Sbjct: 434 DDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVVR 493

Query: 504 APNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 550
           +  S  TEDE+  +++  V  YK+++ V FV+T+PKSPSGK++R+ ++ ++
Sbjct: 494 SNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARL 539

BLAST of Lag0039838 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 2.8e-113
Identity = 209/534 (39.14%), Postives = 331/534 (61.99%), Query Frame = 0

Query: 21  EEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAK 80
           E+ IFRS+LP++ +P  + L  +  +N     +    +   +G+ +TY +V   + + A 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 81  ALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKL 140
            L+ L +++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++ + AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKL 128

Query: 141 VVTNSANFEKVKEL---KLPVIVLGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLC 200
           ++T +   +KVK+    K   I+  ++  +  +++ KL+EA +      V   I   D+ 
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVV---INSDDVV 188

Query: 201 ALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGI 260
           ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M  +   + ++P FHIY +  +
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 248

Query: 261 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSALKL 320
            C  LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V+++DLS+++ 
Sbjct: 249 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR- 308

Query: 321 QAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGR 380
             +M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    +    G 
Sbjct: 309 -TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 368

Query: 381 ILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIG 440
           ++ N E+K +DP++  SLP+N  GE+C+R   +M+GY  + E T  TID +GW+HTGDIG
Sbjct: 369 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 428

Query: 441 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 500
           +IDDD ++FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ DE+AGE+P A 
Sbjct: 429 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 488

Query: 501 VVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 550
           VV       TE+EI ++V+  V  YK++  V FVD IPKSPSGK++R+ ++ ++
Sbjct: 489 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARI 537

BLAST of Lag0039838 vs. ExPASy Swiss-Prot
Match: P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 4.8e-113
Identity = 210/534 (39.33%), Postives = 331/534 (61.99%), Query Frame = 0

Query: 21  EEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAK 80
           E+ IFRS+LP++ +P  + L  +  +N     +    +   +G+ +TY +V   + + A 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 81  ALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKL 140
            L+ L +++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++ + AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKL 128

Query: 141 VVTNSANFEKVKEL---KLPVIVLGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLC 200
           ++T +   +KVK+    K   I+  ++  +  +++ KL+EA +      V   I   D+ 
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVV---IDSDDVV 188

Query: 201 ALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGI 260
           ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M  +   + ++P FHIY +  +
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 248

Query: 261 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSALKL 320
            C  LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V+++DLS+++ 
Sbjct: 249 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR- 308

Query: 321 QAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGR 380
             +M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    +    G 
Sbjct: 309 -TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 368

Query: 381 ILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIG 440
           ++ N E+K +DP++  SLP+N  GE+C+R   +M+GY  + E T  TID +GW+HTGDIG
Sbjct: 369 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 428

Query: 441 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 500
           +IDDD ++FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ DE+AGE+P A 
Sbjct: 429 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 488

Query: 501 VVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 550
           VV       TE+EI ++V+  V  YK++  V FVD IPKSPSGK++R+ ++ K+
Sbjct: 489 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKI 537

BLAST of Lag0039838 vs. ExPASy TrEMBL
Match: A0A6J1G0B4 (4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=4 SV=1)

HSP 1 Score: 1042.7 bits (2695), Expect = 5.4e-301
Identity = 530/559 (94.81%), Postives = 550/559 (98.39%), Query Frame = 0

Query: 1   MPAANNNPDMATCIRDSVEDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEA 60
           M AANNN  MATCIRD VEDEEHIFRSQLPEV VPDDITLPEFVLQNAESYA+NVAFVEA
Sbjct: 48  MSAANNNSAMATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEA 107

Query: 61  VSGKAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGV 120
           VSGKAYTYREVVRDT+RFAKALSSLRL+KGQVV+VVLPNVAEYAIVALGIMAAGGVFSGV
Sbjct: 108 VSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGV 167

Query: 121 NPAAHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEGSMNWHKLLEAAD 180
           NPAAHISEIKKQVEVADAKLVVTNS++FEKVKELKLPVIV+GEELIEGSMNWHKLLEAAD
Sbjct: 168 NPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAAD 227

Query: 181 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTT 240
           RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM GKVTT
Sbjct: 228 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTT 287

Query: 241 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 300
           LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV
Sbjct: 288 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 347

Query: 301 KNPIVEEFDLSALKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGS 360
           KNPIV+EFDLS LKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYGS
Sbjct: 348 KNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGS 407

Query: 361 IGKENLTARKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETS 420
           IGK+NL A+KNTVGRILPNLEVKFIDPDSGRSLPKNTPGE+CVRSQCVMQGYY NEEETS
Sbjct: 408 IGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETS 467

Query: 421 RTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 480
           RTID+KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV
Sbjct: 468 RTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 527

Query: 481 VPLPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKV 540
           VPLPD+EAGEIPAAS+V+APN+KETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKV
Sbjct: 528 VPLPDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKV 587

Query: 541 MRRLIKEKMIEKIRAESSS 560
           MRRLIKEKMIEKIRAE++S
Sbjct: 588 MRRLIKEKMIEKIRAEAAS 606

BLAST of Lag0039838 vs. ExPASy TrEMBL
Match: A0A6J1HUV5 (4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 9.2e-301
Identity = 527/559 (94.28%), Postives = 550/559 (98.39%), Query Frame = 0

Query: 1   MPAANNNPDMATCIRDSVEDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEA 60
           M AANNN  MATCIRDS EDEEHIFRSQLPEV VPDDITLPEFVLQNAE YA+NVAFVEA
Sbjct: 48  MSAANNNSTMATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVAFVEA 107

Query: 61  VSGKAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGV 120
           VSGKAYTYREVVRDT+RFAKALSSLRL+KGQ+V+VVLPNVAEYAIVALGIMAAGGVFSGV
Sbjct: 108 VSGKAYTYREVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGV 167

Query: 121 NPAAHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEGSMNWHKLLEAAD 180
           NPAAHISEIKKQVEVADAKLVVTNS++FEKVKELKLPVIV+GEELIEGSMNWHKLLEAAD
Sbjct: 168 NPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAAD 227

Query: 181 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTT 240
           RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSG+PQEM GKVTT
Sbjct: 228 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTT 287

Query: 241 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 300
           LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV
Sbjct: 288 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 347

Query: 301 KNPIVEEFDLSALKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGS 360
           KNPIV+EFDLS+LKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYG+
Sbjct: 348 KNPIVKEFDLSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGN 407

Query: 361 IGKENLTARKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETS 420
           IGK+NL A+KNTVGRILPNLEVKFIDPDSGRSLPKNTPGE+CVRSQCVMQGYY NEEETS
Sbjct: 408 IGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETS 467

Query: 421 RTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 480
           RTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV
Sbjct: 468 RTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 527

Query: 481 VPLPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKV 540
           VPLPD+EAGEIPAAS+V+APN+KETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKV
Sbjct: 528 VPLPDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKV 587

Query: 541 MRRLIKEKMIEKIRAESSS 560
           MRRLIKEKMIEKIRAE++S
Sbjct: 588 MRRLIKEKMIEKIRAETAS 606

BLAST of Lag0039838 vs. ExPASy TrEMBL
Match: A0A6J1DG05 (4-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 3.4e-295
Identity = 524/559 (93.74%), Postives = 545/559 (97.50%), Query Frame = 0

Query: 1   MPAANNNPDMATCIRDSVEDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEA 60
           MP AN +  M T IRD VEDEEHIFRSQLPEV VPDDITLPEFVLQNAESYAENVAFVEA
Sbjct: 52  MPVANISA-MGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEA 111

Query: 61  VSGKAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGV 120
           VSGKAYTYREVVRDT RFAKALSSLRLRKGQVV+VVLPNVAEYAIVALGIMAAGGVFSGV
Sbjct: 112 VSGKAYTYREVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGV 171

Query: 121 NPAAHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEGSMNWHKLLEAAD 180
           NPAAHISEIKKQVE ADAKLVVTNS NFEKVKELKLPVIVLGEELIEGSMNWHKLLEAAD
Sbjct: 172 NPAAHISEIKKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAAD 231

Query: 181 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTT 240
           RAGNN VKEDIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E EGKVTT
Sbjct: 232 RAGNNHVKEDIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTT 291

Query: 241 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 300
           LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV
Sbjct: 292 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 351

Query: 301 KNPIVEEFDLSALKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGS 360
           KNPIVEEFDLS+LKLQAIMTAAAPLAPE+QTAFEKKFPGVDVQEAYGLTEHSCITLNYGS
Sbjct: 352 KNPIVEEFDLSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGS 411

Query: 361 IGKENLTARKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETS 420
           IGKENLTA+KNTVGRILPNLEVKFIDP+SGRSLPKNTPGE+CVRSQCVMQGYY NEEETS
Sbjct: 412 IGKENLTAKKNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETS 471

Query: 421 RTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 480
           RTIDN+GW+HTGDIGYIDD+GDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV
Sbjct: 472 RTIDNRGWLHTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 531

Query: 481 VPLPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKV 540
           VPLPDEEAGEIPAASVVIAPNSKETE+EII+YVASNVA+YKKVR+VHFVD+IPKSPSGKV
Sbjct: 532 VPLPDEEAGEIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKV 591

Query: 541 MRRLIKEKMIEKIRAESSS 560
           MRRLIKEKMI+KIRA+++S
Sbjct: 592 MRRLIKEKMIDKIRAKTAS 609

BLAST of Lag0039838 vs. ExPASy TrEMBL
Match: A0A6J1DEE1 (4-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 3.4e-295
Identity = 524/559 (93.74%), Postives = 545/559 (97.50%), Query Frame = 0

Query: 1   MPAANNNPDMATCIRDSVEDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEA 60
           MP AN +  M T IRD VEDEEHIFRSQLPEV VPDDITLPEFVLQNAESYAENVAFVEA
Sbjct: 1   MPVANISA-MGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEA 60

Query: 61  VSGKAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGV 120
           VSGKAYTYREVVRDT RFAKALSSLRLRKGQVV+VVLPNVAEYAIVALGIMAAGGVFSGV
Sbjct: 61  VSGKAYTYREVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGV 120

Query: 121 NPAAHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEGSMNWHKLLEAAD 180
           NPAAHISEIKKQVE ADAKLVVTNS NFEKVKELKLPVIVLGEELIEGSMNWHKLLEAAD
Sbjct: 121 NPAAHISEIKKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAAD 180

Query: 181 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTT 240
           RAGNN VKEDIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E EGKVTT
Sbjct: 181 RAGNNHVKEDIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTT 240

Query: 241 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 300
           LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV
Sbjct: 241 LGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALV 300

Query: 301 KNPIVEEFDLSALKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGS 360
           KNPIVEEFDLS+LKLQAIMTAAAPLAPE+QTAFEKKFPGVDVQEAYGLTEHSCITLNYGS
Sbjct: 301 KNPIVEEFDLSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGS 360

Query: 361 IGKENLTARKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETS 420
           IGKENLTA+KNTVGRILPNLEVKFIDP+SGRSLPKNTPGE+CVRSQCVMQGYY NEEETS
Sbjct: 361 IGKENLTAKKNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETS 420

Query: 421 RTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 480
           RTIDN+GW+HTGDIGYIDD+GDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV
Sbjct: 421 RTIDNRGWLHTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAV 480

Query: 481 VPLPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKV 540
           VPLPDEEAGEIPAASVVIAPNSKETE+EII+YVASNVA+YKKVR+VHFVD+IPKSPSGKV
Sbjct: 481 VPLPDEEAGEIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKV 540

Query: 541 MRRLIKEKMIEKIRAESSS 560
           MRRLIKEKMI+KIRA+++S
Sbjct: 541 MRRLIKEKMIDKIRAKTAS 558

BLAST of Lag0039838 vs. ExPASy TrEMBL
Match: A0A0A0KRZ5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589320 PE=4 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 1.5e-290
Identity = 509/550 (92.55%), Postives = 539/550 (98.00%), Query Frame = 0

Query: 10  MATCIRDSVEDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 69
           MATC +DSV+DEEHIF SQLPEV VP DITLPEFVLQNAESYAENVAFVEA+SGKAYTYR
Sbjct: 1   MATCNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYR 60

Query: 70  EVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 129
           EV+RDTNRF+KAL SLRL+KG VV+VVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 61  EVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 130 KKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNNFVKE 189
           KKQVEVA+AKLVVTN+ANFEKV+ELKLPVI+L EEL+EG+MNWHKLLEAADRAGNNFVKE
Sbjct: 121 KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE 180

Query: 190 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGLIPFFHI 249
           +IKQTDLCALPFSSGTTGVSKGVMLTHRNLVAN+CSTLSGVPQEMEGKVTTLGLIPFFHI
Sbjct: 181 EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 250 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 309
           YGITGICC+TLRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIVEEFD
Sbjct: 241 YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD 300

Query: 310 LSALKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAR 369
           LS+LKLQAIMTAAAPLAPELQTAFE+KFPGVDVQEAYGLTEH CITLNYGSIGKENL+A+
Sbjct: 301 LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAK 360

Query: 370 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWM 429
           KNTVGRILPNLEVKFIDPD+GRSLPKNTPGE+CVRSQCVMQGYYKNEEETSRTID KGWM
Sbjct: 361 KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWM 420

Query: 430 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 489
           HTGDIGYIDDDG+VFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 421 HTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480

Query: 490 EIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 549
           EIPAASVV+APNSKETE++IIK+VASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM
Sbjct: 481 EIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540

Query: 550 IEKIRAESSS 560
           IEKIRA+ S+
Sbjct: 541 IEKIRADESA 550

BLAST of Lag0039838 vs. TAIR 10
Match: AT1G62940.1 (acyl-CoA synthetase 5 )

HSP 1 Score: 792.3 bits (2045), Expect = 2.5e-229
Identity = 396/532 (74.44%), Postives = 457/532 (85.90%), Query Frame = 0

Query: 19  EDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRF 78
           ED E+IFRS  P V +PD +TLPEFVLQ  E Y ENVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 79  AKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADA 138
           AKAL+SL LRKGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 139 KLVVTNSANFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCA 198
           + ++T++ N+EKVK L LPVIVLGEE IEG++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 199 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCA 258
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  EM G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 259 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSALKLQAI 318
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 319 MTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGRILP 378
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEHSCITL +G   K    A++N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 379 NLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 438
           NLEVKFIDPD+GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 439 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVI 498
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VVI
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 499 APNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMI 551
            P + E E++I+ +VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of Lag0039838 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 399.4 bits (1025), Expect = 4.6e-111
Identity = 207/551 (37.57%), Postives = 333/551 (60.44%), Query Frame = 0

Query: 5   NNNPDMATCIRDSVEDEEHIFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEAVSGK 64
           N+  D   C  D       IFRS+LP++ +P+ + L +++ +N   +A     +   +G+
Sbjct: 9   NDQNDQKQCSNDV------IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGE 68

Query: 65  AYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAA 124
            YTY +V   + + A  L +L +++  VV+++LPN  E  +  L     G + +  NP  
Sbjct: 69  VYTYADVHVTSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFF 128

Query: 125 HISEIKKQVEVADAKLVVTNSANFEKVKELK----LPVIVLGEELIEGSMNWHKLLEAAD 184
             +EI KQ + + AKL+VT S   +K+K L+    L V    + + E  + + +L ++ +
Sbjct: 129 TPAEISKQAKASAAKLIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEE 188

Query: 185 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM--EGKV 244
              ++ + E I   D+ ALPFSSGTTG+ KGVMLTH+ LV ++   + G    +      
Sbjct: 189 PRVDS-IPEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDD 248

Query: 245 TTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILA 304
             L ++P FHIY +  I   +LR    +++M +F++   +  +   +VT A +VPPI+LA
Sbjct: 249 VILCVLPMFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLA 308

Query: 305 LVKNPIVEEFDLSALKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNY 364
           + K+P  E++DLS++++  + + AAPL  EL+ A   KFP   + + YG+TE   +    
Sbjct: 309 IAKSPETEKYDLSSVRM--VKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMS 368

Query: 365 GSIGKENLTARKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEE 424
               KE    +    G ++ N E+K +DPD+G SLP+N PGE+C+R   +M+GY  +   
Sbjct: 369 LGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLA 428

Query: 425 TSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDA 484
           T+ TID  GW+HTGD+G+IDDD ++FIVDR+KELIKYKGFQVAPAELE++L+ HP I D 
Sbjct: 429 TASTIDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDV 488

Query: 485 AVVPLPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSG 544
           AVV + +E+AGE+P A VV + +S  +EDEI ++V+  V  YK++  V F D+IPK+PSG
Sbjct: 489 AVVAMKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSG 548

Query: 545 KVMRRLIKEKM 550
           K++R+ ++ ++
Sbjct: 549 KILRKDLRARL 550

BLAST of Lag0039838 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 388.7 bits (997), Expect = 8.2e-108
Identity = 198/536 (36.94%), Postives = 322/536 (60.07%), Query Frame = 0

Query: 24  IFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAKALS 83
           IFRS+LP++ +P+ ++L +++ QN   +A     +   +G  YTY +V   + + A    
Sbjct: 24  IFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFH 83

Query: 84  SLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 143
            L + +  VV+++LPN  E+ +  L     G   +  NP    +EI KQ + ++ KL++T
Sbjct: 84  KLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIIT 143

Query: 144 NSANFEKVKELK----LPVIVLGEE----LIEGSMNWHKLLEAADRAGNNFVKEDIKQTD 203
            +   +K+K L+    + ++ + +     + EG + + +L ++   A       +I   D
Sbjct: 144 EARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDD 203

Query: 204 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM--EGKVTTLGLIPFFHIYGIT 263
           + ALP+SSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY + 
Sbjct: 204 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALN 263

Query: 264 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSAL 323
            I    LR    +++M +F++   +  +   +VT AP+VPPI+LA+ K+   E++DLS++
Sbjct: 264 SIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSI 323

Query: 324 KLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTV 383
           ++  + + AAPL  EL+ A   KFP   + + YG+TE   +        KE    +    
Sbjct: 324 RV--VKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 383

Query: 384 GRILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGD 443
           G ++ N E+K +DPD+G SL +N PGE+C+R   +M+GY  N   T+ TID  GW+HTGD
Sbjct: 384 GTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGD 443

Query: 444 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPA 503
           IG IDDD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I D AVV + +E AGE+P 
Sbjct: 444 IGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPV 503

Query: 504 ASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 550
           A VV + +S+ +ED++ ++V+  V  YK++  V F ++IPK+PSGK++R+ ++ K+
Sbjct: 504 AFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of Lag0039838 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 380.2 bits (975), Expect = 2.9e-105
Identity = 198/553 (35.80%), Postives = 330/553 (59.67%), Query Frame = 0

Query: 16  DSVEDEEH--IFRSQLPEVLVPDDITLPEFVLQ----NAESYAENVAFVEAVSGKAYTYR 75
           D  E+  H  IFRS+LP++ +P+ + L ++V Q    + +  +     ++  +G+  TY 
Sbjct: 18  DEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYA 77

Query: 76  EVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 135
           +V  +  R A  +  L +R G VV+++LPN  E+A+  L +   G V +  NP     EI
Sbjct: 78  DVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEI 137

Query: 136 KKQVEVADAKLVVTNSANFEKVKELKLPVIVL------GEELI-----EGSMNWHKLLEA 195
            KQ + + AK+++T     +K+  LK   +++      G+  +     +G +++ +L +A
Sbjct: 138 AKQAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQA 197

Query: 196 ADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEME--G 255
            +      +K  I   D  A+P+SSGTTG+ KGVM+TH+ LV ++   + G    +    
Sbjct: 198 DE---TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTA 257

Query: 256 KVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPII 315
               L  +P FHIY +  +  + +R    ++++ RF+L   +  +   +VT  P+ PP++
Sbjct: 258 NDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVV 317

Query: 316 LALVKNPIVEEFDLSALKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITL 375
           LA +K+P  E +DLS++++  +++ AA L  EL+ A   KFP     + YG+TE   +  
Sbjct: 318 LAFIKSPETERYDLSSVRI--MLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTVAK 377

Query: 376 NYGSIGKENLTARKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNE 435
           +  +  K     +    G ++ N E+K +D ++G SLP+N  GE+CVR   +M+GY  + 
Sbjct: 378 SL-AFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDP 437

Query: 436 EETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIE 495
           E T+RTID  GW+HTGDIG++DDD ++FIVDR+KELIK+KG+QVAPAELEA+L++HPSI+
Sbjct: 438 EATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSID 497

Query: 496 DAAVVPLPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSP 550
           DAAVV + DE A E+P A V  +  S+ TED++  YV   V HYK++++V F++ IPK+ 
Sbjct: 498 DAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAV 557

BLAST of Lag0039838 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 379.8 bits (974), Expect = 3.8e-105
Identity = 204/531 (38.42%), Postives = 322/531 (60.64%), Query Frame = 0

Query: 24  IFRSQLPEVLVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAKALS 83
           IFRS+LP++ +P+ + L  +  +   S ++    +   +GK+YTY E      R A  L 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 84  SLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 143
            L +RKG V++++L N AE+    +G    G V +  NP     E+ KQ++ + AKL++T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 144 NSANFEKVKEL--KLPVIVLGEELIEGSMNWHKLLEAADRAGNNFVKE-DIKQTDLCALP 203
           +S   +K+K L   L +I   E   E  + +  L+   D   N F +  DI   D  ALP
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLI--TDDETNPFQETVDIGGDDAAALP 213

Query: 204 FSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGICCA 263
           FSSGTTG+ KGV+LTH++L+ ++   + G      ++     L ++P FHIY +  +   
Sbjct: 214 FSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLN 273

Query: 264 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSALKLQAI 323
           +LR+   V++M +F++   ++ +    VT A +VPP+++AL KNP V  +DLS+++   +
Sbjct: 274 SLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF--V 333

Query: 324 MTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGRILP 383
           ++ AAPL  ELQ +  ++ P   + + YG+TE   +        KE +  +  + G ++ 
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 384 NLEVKFIDPDSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 443
           N E+K +  ++  SL  N PGE+C+R Q +M+ Y  + E TS TID +GW+HTGDIGY+D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 444 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVI 503
           +D ++FIVDR+KE+IK+KGFQV PAELE++L+ H SI DAAVVP  DE AGE+P A VV 
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 504 APNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 550
           +  +  TE+++ +YVA  V  YK++  V FV +IPKSPSGK++R+ +K K+
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560