Lag0039810 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0039810
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Chaperone DnaJ domain protein
Location	chr13: 160941 .. 161912 (+)
RNA-Seq Expression	Lag0039810
Synteny	Lag0039810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGGGCGACGAAGCTAGAATCTTGCTTGGATTCTCTCCCGGCTCCTGCCCGTCTCCTTCGCAGGTGCTGTATTTAGAATTTACACATCTCTTCTCTTACAAATTCCTCTGTCTCCTTTAATTCTGATCAACGCATGTATAGTATGAATAGATTTTCAAGATGCTTCGGGTTCGGTTAAAGCTGTATTTCACTATTTCTGTTCTACATTGCAGCTTAAACAAGCTTACAAAAGGAAGGTATGGGACTCTCATCCTGACCTGTTCCCTGCTCATGAAAAGTCTCAGGCGGAGTCCAAATTCAAACTCGTAAGGATTAAGGAATACGTTCTCTTGTGTTTTCACCAGCTTATGGTACTTTGCATACTATAATGCGTTCATTTTGTGAATCCTATCTGAATACATGCATCTGCTGCAGATTTCCGAAGCTTATTCATGCCTCTTATCTGGTAATTCTCCTATATCTACAACTGATCTCAGACATGCTACAAGACATGGAAAAAAAAACTACTAATTGAATTCCCTGTTTCTCCGATAGTGTACTTCCAAATTTGCTTCTGGTCTAAACTTCTTAATCTTTGCAGGTTCCCGAAAAGGAGATTCACATTCAGGTAATTTATATAAATCCTTCAATATTACTTAATTATTACTAGTTTTGTTAGTCCATTCTAGGATACGATTTGATGAGTGATTCTGGACAGCCACATATGAACGGGTTGTCAGAAGGGGGGTTCCCGTCTCATACGGAGGAAGAAGAAACCATGCCTTAATCAAGCTTCCCTTTCTATTGCTGATTCTGGGGACTTTTTCACTCGGAGGATTAAATGTTATCCGGTGAGTCCCAAGCATCAACATGTCATGATTTGTTAAAGAAATCTACAAGTTTTAAGAAGTTGCTCGCACTACTGCATTTTTCAGGGCATACAAAAAGCAGAAGGAAACGTATCCTTCTCACAATCCTTTTCTTCCTTAA

mRNA sequence

ATGCAGGGCGACGAAGCTAGAATCTTGCTTGGATTCTCTCCCGGCTCCTGCCCGTCTCCTTCGCAGCTTAAACAAGCTTACAAAAGGAAGGTATGGGACTCTCATCCTGACCTGTTCCCTGCTCATGAAAAGTCTCAGGCGGAGTCCAAATTCAAACTCATTTCCGAAGCTTATTCATGCCTCTTATCTGGTTCCCGAAAAGGAGATTCACATTCAGCCACATATGAACGGGTTGTCAGAAGGGGGGTTCCCGTCTCATACGGAGGAAGAAGAAACCATGCCTTAATCAAGCTTCCCTTTCTATTGCTGATTCTGGGGACTTTTTCACTCGGAGGATTAAATGTTATCCGGGCATACAAAAAGCAGAAGGAAACGTATCCTTCTCACAATCCTTTTCTTCCTTAA

Coding sequence (CDS)

ATGCAGGGCGACGAAGCTAGAATCTTGCTTGGATTCTCTCCCGGCTCCTGCCCGTCTCCTTCGCAGCTTAAACAAGCTTACAAAAGGAAGGTATGGGACTCTCATCCTGACCTGTTCCCTGCTCATGAAAAGTCTCAGGCGGAGTCCAAATTCAAACTCATTTCCGAAGCTTATTCATGCCTCTTATCTGGTTCCCGAAAAGGAGATTCACATTCAGCCACATATGAACGGGTTGTCAGAAGGGGGGTTCCCGTCTCATACGGAGGAAGAAGAAACCATGCCTTAATCAAGCTTCCCTTTCTATTGCTGATTCTGGGGACTTTTTCACTCGGAGGATTAAATGTTATCCGGGCATACAAAAAGCAGAAGGAAACGTATCCTTCTCACAATCCTTTTCTTCCTTAA

Protein sequence

MQGDEARILLGFSPGSCPSPSQLKQAYKRKVWDSHPDLFPAHEKSQAESKFKLISEAYSCLLSGSRKGDSHSATYERVVRRGVPVSYGGRRNHALIKLPFLLLILGTFSLGGLNVIRAYKKQKETYPSHNPFLP

Homology

[Close]

BLAST of Lag0039810 vs. NCBI nr
Match: KAG6573609.1 (hypothetical protein SDJN03_27496, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 251.5 bits (641), Expect = 4.0e-63
Identity = 122/134 (91.04%), Postives = 128/134 (95.52%), Query Frame = 0


Query: 1   MQGDEARILLGFSPGSCPSPSQLKQAYKRKVWDSHPDLFPAHEKSQAESKFKLISEAYSC 60
           MQGDEARILLGFSPGS PSPSQ+K+AYKRKVWDSHPDLFPAH+KSQAESKFKLISEAYS 
Sbjct: 1   MQGDEARILLGFSPGSRPSPSQVKEAYKRKVWDSHPDLFPAHQKSQAESKFKLISEAYSF 60

Query: 61  LLSGSRKGDSHSATYERVVRRGVPVSYGGRRNHALIKLPFLLLILGTFSLGGLNVIRAYK 120
           LLSG+R+GDSHSA+YERVVRRGVPVS GGRRNHALIKLPFLLLILGT SLGGLNV RAYK
Sbjct: 61  LLSGARRGDSHSASYERVVRRGVPVSQGGRRNHALIKLPFLLLILGTVSLGGLNVSRAYK 120

Query: 121 KQKETYPSHNPFLP 135
           KQKE YPSHNPFLP
Sbjct: 121 KQKEAYPSHNPFLP 134

[Close]

BLAST of Lag0039810 vs. NCBI nr
Match: XP_004135355.1 (uncharacterized protein LOC101221514 [Cucumis sativus] >KGN51624.1 hypothetical protein Csa_009442 [Cucumis sativus])

HSP 1 Score: 250.4 bits (638), Expect = 8.9e-63
Identity = 121/134 (90.30%), Postives = 126/134 (94.03%), Query Frame = 0


Query: 1   MQGDEARILLGFSPGSCPSPSQLKQAYKRKVWDSHPDLFPAHEKSQAESKFKLISEAYSC 60
           MQGDEAR LLGFSPGS PS SQ+K+AYKR VWD+HPDLFPAH+K QAESKFKLISEAYSC
Sbjct: 1   MQGDEARTLLGFSPGSRPSSSQVKEAYKRMVWDTHPDLFPAHQKPQAESKFKLISEAYSC 60

Query: 61  LLSGSRKGDSHSATYERVVRRGVPVSYGGRRNHALIKLPFLLLILGTFSLGGLNVIRAYK 120
           LLSGSR GDSHSATYERVVRRGVPVS+GGRRNHALIKLPFLLLILGT SLGGLNV RAYK
Sbjct: 61  LLSGSRNGDSHSATYERVVRRGVPVSHGGRRNHALIKLPFLLLILGTVSLGGLNVSRAYK 120

Query: 121 KQKETYPSHNPFLP 135
           KQKETYPSHNPFLP
Sbjct: 121 KQKETYPSHNPFLP 134

[Close]

BLAST of Lag0039810 vs. NCBI nr
Match: XP_022967118.1 (uncharacterized protein LOC111466618 [Cucurbita maxima])

HSP 1 Score: 250.0 bits (637), Expect = 1.2e-62
Identity = 121/134 (90.30%), Postives = 127/134 (94.78%), Query Frame = 0


Query: 1   MQGDEARILLGFSPGSCPSPSQLKQAYKRKVWDSHPDLFPAHEKSQAESKFKLISEAYSC 60
           MQGDEARILLGFSPGS PSPSQ+K+AYKRKVWDSHPDLFPAH+KSQAESKFKLISEAYS 
Sbjct: 1   MQGDEARILLGFSPGSRPSPSQVKEAYKRKVWDSHPDLFPAHQKSQAESKFKLISEAYSF 60

Query: 61  LLSGSRKGDSHSATYERVVRRGVPVSYGGRRNHALIKLPFLLLILGTFSLGGLNVIRAYK 120
           LLSG+R+GDSHS +YERVVRRGVPVS GGRRNHALIKLPFLLLILGT SLGGLNV RAYK
Sbjct: 61  LLSGARRGDSHSTSYERVVRRGVPVSQGGRRNHALIKLPFLLLILGTVSLGGLNVSRAYK 120

Query: 121 KQKETYPSHNPFLP 135
           KQKE YPSHNPFLP
Sbjct: 121 KQKEAYPSHNPFLP 134

[Close]

BLAST of Lag0039810 vs. NCBI nr
Match: XP_023541159.1 (uncharacterized protein LOC111801402 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 250.0 bits (637), Expect = 1.2e-62
Identity = 121/134 (90.30%), Postives = 127/134 (94.78%), Query Frame = 0


Query: 1   MQGDEARILLGFSPGSCPSPSQLKQAYKRKVWDSHPDLFPAHEKSQAESKFKLISEAYSC 60
           MQGDEARILLGFSPGS PSPSQ+K+AYKRKVWDSHPDLFPAH+KSQAESKFKLISEAYS 
Sbjct: 1   MQGDEARILLGFSPGSRPSPSQVKEAYKRKVWDSHPDLFPAHQKSQAESKFKLISEAYSF 60

Query: 61  LLSGSRKGDSHSATYERVVRRGVPVSYGGRRNHALIKLPFLLLILGTFSLGGLNVIRAYK 120
           LLSG+R+GDSHSA+YERVVRRGVPVS GGRRNHALIKLPFL LILGT SLGGLNV RAYK
Sbjct: 61  LLSGARRGDSHSASYERVVRRGVPVSQGGRRNHALIKLPFLFLILGTVSLGGLNVSRAYK 120

Query: 121 KQKETYPSHNPFLP 135
           KQKE YPSHNPFLP
Sbjct: 121 KQKEAYPSHNPFLP 134

[Close]

BLAST of Lag0039810 vs. NCBI nr
Match: XP_022945242.1 (uncharacterized protein LOC111449543 isoform X1 [Cucurbita moschata])

HSP 1 Score: 250.0 bits (637), Expect = 1.2e-62
Identity = 121/134 (90.30%), Postives = 127/134 (94.78%), Query Frame = 0


Query: 1   MQGDEARILLGFSPGSCPSPSQLKQAYKRKVWDSHPDLFPAHEKSQAESKFKLISEAYSC 60
           MQGDEARILLGFSPGS PSPSQ+K+AYKRKVWDSHPDLFP H+KSQAESKFKLISEAYS 
Sbjct: 1   MQGDEARILLGFSPGSRPSPSQVKEAYKRKVWDSHPDLFPVHQKSQAESKFKLISEAYSF 60

Query: 61  LLSGSRKGDSHSATYERVVRRGVPVSYGGRRNHALIKLPFLLLILGTFSLGGLNVIRAYK 120
           LLSG+R+GDSHSA+YERVVRRGVPVS GGRRNHALIKLPFLLLILGT SLGGLNV RAYK
Sbjct: 61  LLSGARRGDSHSASYERVVRRGVPVSQGGRRNHALIKLPFLLLILGTVSLGGLNVSRAYK 120

Query: 121 KQKETYPSHNPFLP 135
           KQKE YPSHNPFLP
Sbjct: 121 KQKEAYPSHNPFLP 134

[Close]

BLAST of Lag0039810 vs. ExPASy Swiss-Prot
Match: Q2GLU9 (Chaperone protein DnaJ OS=Anaplasma phagocytophilum (strain HZ) OX=212042 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 3.0e-05
Identity = 30/83 (36.14%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 9  LLGFSPGSCPSPSQLKQAYKRKVWDSHPDLFPAHEKSQAESKFKLISEAYSCLLSGSRKG 68
          LLG S G+  S  ++K++Y++KV++ HPD  P ++  +AE KFK ISEAY  L    ++ 
Sbjct: 9  LLGVSRGA--SEEEIKKSYRKKVFEYHPDRNPGNK--EAEEKFKKISEAYDVLTDSDKR- 68

Query: 69 DSHSATYERVVRRGVPVSYGGRR 92
              A Y+R          GG +
Sbjct: 69 ----AAYDRYGHSAFANGMGGNQ 82

[Close]

BLAST of Lag0039810 vs. ExPASy Swiss-Prot
Match: Q6AYU3 (DnaJ homolog subfamily B member 6 OS=Rattus norvegicus OX=10116 GN=Dnajb6 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.4e-04
Identity = 27/75 (36.00%), Postives = 43/75 (57.33%), Query Frame = 0


Query: 19 SPSQLKQAYKRKVWDSHPDLFPAHEKSQAESKFKLISEAYSCLLSGSRKGDSHSATYERV 78
          SP  +K+AY+++    HPD  P   K +AE KFK ++EAY  +LS ++K D     Y++ 
Sbjct: 15 SPEDIKKAYRKQALKWHPDKNP-ENKEEAERKFKQVAEAYE-VLSDAKKRD----IYDKY 74

Query: 79 VRRGVPVSYGGRRNH 94
           + G+    GG  +H
Sbjct: 75 GKEGLNGGGGGGGSH 83

[Close]

BLAST of Lag0039810 vs. ExPASy Swiss-Prot
Match: B0TQC1 (Chaperone protein DnaJ OS=Shewanella halifaxensis (strain HAW-EB4) OX=458817 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 7.5e-04
Identity = 27/75 (36.00%), Postives = 39/75 (52.00%), Query Frame = 0


Query: 15 GSCPSPSQLKQAYKRKVWDSHPDLFPAHEKSQAESKFKLISEAYSCLLSGSRKGDSHSAT 74
          G   S  ++K+AYKR     HPD  P ++  +AE+ FK + EAY  L  G +K     A 
Sbjct: 13 GRDASEREIKKAYKRLAMKFHPDRNPGNK--EAEASFKEVKEAYEILTDGDKK-----AA 72

Query: 75 YERVVRRGVPVSYGG 90
          Y++    GV  + GG
Sbjct: 73 YDQFGHAGVDPNRGG 80

[Close]

BLAST of Lag0039810 vs. ExPASy Swiss-Prot
Match: Q835R5 (Chaperone protein DnaJ OS=Enterococcus faecalis (strain ATCC 700802 / V583) OX=226185 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 9.8e-04
Identity = 27/81 (33.33%), Postives = 45/81 (55.56%), Query Frame = 0


Query: 9  LLGFSPGSCPSPSQLKQAYKRKVWDSHPDLFPAHEKSQAESKFKLISEAYSCLLSGSRKG 68
          +LG + G+  S  ++K+AY++     HPD+   ++++ AE KFK +SEAY  L    +K 
Sbjct: 10 VLGLAKGA--SDDEIKKAYRKLSKKYHPDI---NKEADAEEKFKEVSEAYEVLSDPQKK- 69

Query: 69 DSHSATYERVVRRGVPVSYGG 90
              A Y++    G   +YGG
Sbjct: 70 ----AAYDQYGHAGTDPNYGG 80

[Close]

BLAST of Lag0039810 vs. ExPASy Swiss-Prot
Match: Q6ME07 (Chaperone protein DnaJ OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 9.8e-04
Identity = 30/94 (31.91%), Postives = 49/94 (52.13%), Query Frame = 0


Query: 19  SPSQLKQAYKRKVWDSHPDLFPAHEKSQAESKFKLISEAYSCLLSGSRKGDSHSATYERV 78
           +P ++K+AY++K    HPD  P    + AE +FK ISEAY  +LS  +K       Y+R 
Sbjct: 15  TPEEIKKAYRKKAVQYHPDKNPG--DADAEKRFKEISEAYE-VLSDEKK----RQVYDRY 74

Query: 79  VRRGVPVSYGGRRNHALIKLPFLLLILGTFSLGG 113
            +  +  + GG +  + +    L   +G F +GG
Sbjct: 75  GKEALQGAAGGGQGFSSMD-EALRTFMGAFGMGG 100

The following BLAST results are available for this feature:

BLAST of Lag0039810 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6573609.1	4.0e-63	91.04	hypothetical protein SDJN03_27496, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_004135355.1	8.9e-63	90.30	uncharacterized protein LOC101221514 [Cucumis sativus] >KGN51624.1 hypothetical ...	[more]
XP_022967118.1	1.2e-62	90.30	uncharacterized protein LOC111466618 [Cucurbita maxima]	[more]
XP_023541159.1	1.2e-62	90.30	uncharacterized protein LOC111801402 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022945242.1	1.2e-62	90.30	uncharacterized protein LOC111449543 isoform X1 [Cucurbita moschata]	[more]

BLAST of Lag0039810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2GLU9	3.0e-05	36.14	Chaperone protein DnaJ OS=Anaplasma phagocytophilum (strain HZ) OX=212042 GN=dna...	[more]
Q6AYU3	4.4e-04	36.00	DnaJ homolog subfamily B member 6 OS=Rattus norvegicus OX=10116 GN=Dnajb6 PE=1 S...	[more]
B0TQC1	7.5e-04	36.00	Chaperone protein DnaJ OS=Shewanella halifaxensis (strain HAW-EB4) OX=458817 GN=...	[more]
Q835R5	9.8e-04	33.33	Chaperone protein DnaJ OS=Enterococcus faecalis (strain ATCC 700802 / V583) OX=2...	[more]
Q6ME07	9.8e-04	31.91	Chaperone protein DnaJ OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 4..66 e-value: 2.7E-5 score: 33.6
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 11..63 e-value: 1.5E-8 score: 34.6
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 5..79 score: 10.894135
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 9..61 e-value: 1.42736E-8 score: 45.9993
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 8..82 e-value: 4.3E-10 score: 41.7
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 9..67
None	No IPR available	PANTHER	PTHR43948	FAMILY NOT NAMED	coord: 1..134
None	No IPR available	PANTHER	PTHR43948:SF14	OS07G0637100 PROTEIN	coord: 1..134

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0039810.1	Lag0039810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061077	chaperone-mediated protein folding
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0051087	chaperone binding
molecular_function	GO:0044183	protein folding chaperone
molecular_function	GO:0051082	unfolded protein binding