Lag0039784 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0039784 vs. NCBI nr
Match: XP_023535809.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535810.1 protein NRT1/ PTR FAMILY 5.10-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023535814.1 protein NRT1/ PTR FAMILY 5.10-like isoform X6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 923.7 bits (2386), Expect = 7.5e-265
Identity = 469/562 (83.45%), Postives = 506/562 (90.04%), Query Frame = 0

Query: 1   MEAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           MEAPLL++TV+GAVDYNGRPVRRS+SGGWRSA+LIIGVEVAERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LRQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATL 120
           L QSTATAAENVNAWSG ASLLPLLGAFLADSFLGRYRTIA+S+VLYILGLGLLT+SATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S CQQ+EKS+ CSP L QV+LFF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 SKAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTY 240
           SKAKSSFFNWWYFCISLATF TVNILNYVQDNLSWSLGFGIPC++MV AL+VF LGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 S----------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIV 300
                                  +     SSEIA EEETRGLLPHH+SQQFRFL+KALIV
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEETRGLLPHHSSQQFRFLNKALIV 300

Query: 301 HNGSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPG 360
            N SKE  H C++SEVEEAKAVLRL PIWVTCLAYA+VFSQSSTFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTISEVEEAKAVLRLLPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMII 420
           F++PAA+LQSFI  SIV+SLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLS+LSM+I
Sbjct: 361 FDVPAASLQSFIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSSLSMVI 420

Query: 421 AALVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AALVEIKRL+TAQEYGLVDLPKATVPL++WWLVPQYVLFGVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRSIGLSLYLSIFGVGNFLSSFLISAIE L+ GDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

BLAST of Lag0039784 vs. NCBI nr
Match: XP_023535811.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 921.4 bits (2380), Expect = 3.7e-264
Identity = 467/555 (84.14%), Postives = 501/555 (90.27%), Query Frame = 0

Query: 1   MEAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           MEAPLL++TV+GAVDYNGRPVRRS+SGGWRSA+LIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LRQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATL 120
           L QSTATAAENVN WSGVASLLPLLGAFLADSF+GRYRTIA+SSVLYILGLGLLT+SATL
Sbjct: 61  LGQSTATAAENVNVWSGVASLLPLLGAFLADSFIGRYRTIALSSVLYILGLGLLTVSATL 120

Query: 121 PSPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S CQQ+EKS+ CSP L QV+LFF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 SKAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTY 240
           SKAKSSFFNWWYFCISLATF TVNILNYVQDNLSWSLGFGIPC++MV AL+VF LGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 S----------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIV 300
                                  +     SSEIA EEE RGLLPHH+SQQFRFL+KALIV
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 301 HNGSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPG 360
            N SKE  H C++ EVEEAKAVLRL PIWVTCLAYA+VFSQSSTFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTIREVEEAKAVLRLVPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMII 420
           FE+PAA+LQS I  SIV+SLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSM+I
Sbjct: 361 FEVPAASLQSVIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AALVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AALVEIKRL+TAQEYGLVDLPKATVPL++WWLVPQYVLFGVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGL 534
           GLRSIGLSLYLSIFGVGNFLSSFLISAIE+L+ GDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

BLAST of Lag0039784 vs. NCBI nr
Match: XP_022925410.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita moschata])

HSP 1 Score: 919.5 bits (2375), Expect = 1.4e-263
Identity = 468/562 (83.27%), Postives = 504/562 (89.68%), Query Frame = 0

Query: 1   MEAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           MEAPLL++TV+ AVDYNGRPVRRS+SGGWRSA+LIIGVEVAERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVECAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LRQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATL 120
           L QSTATAAENVNAWSG ASLLPLLGAFLADSFLGRYRTIA+S+VLYILGLGLLT+SATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S CQQ+EKS+ CSP L QV+LFFFSLY+VAFAQGGHKPCVQAFGADQFD QHPEE
Sbjct: 121 HSPSISGCQQTEKSLPCSPGLFQVILFFFSLYVVAFAQGGHKPCVQAFGADQFDAQHPEE 180

Query: 181 SKAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTY 240
           SKAKSSFFNWWYFCISLATF TVNILNYVQDNLSWSLGFGIPC++MV AL+VF LGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 S----------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIV 300
                                  +     SSEIA EEE RGLLPHH+SQQFRFL+KAL+V
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKALLV 300

Query: 301 HNGSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPG 360
            N SKE  H C++SEVEEAKAVLRL PIWVTCLAYAIVFSQSSTFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMII 420
           F++PAA+LQSFIS SIV+SLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSM+I
Sbjct: 361 FDVPAASLQSFISLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AALVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AALVEIKRL+TAQEYGLVDLPKATVPL++WWLVPQYVLFGVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRSIGLSLYLSIFGVGNFLSSFLISAIE L+ GDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

BLAST of Lag0039784 vs. NCBI nr
Match: XP_023535812.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 919.1 bits (2374), Expect = 1.8e-263
Identity = 466/555 (83.96%), Postives = 501/555 (90.27%), Query Frame = 0

Query: 1   MEAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           MEAPLL++TV+GAVDYNGRPVRRS+SGGWRSA+LIIGVEVAERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LRQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATL 120
           L QSTATAAENVNAWSG ASLLPLLGAFLADSFLGRYRTIA+S+VLYILGLGLLT+SATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S CQQ+EKS+ CSP L QV+LFF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 SKAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTY 240
           SKAKSSFFNWWYFCISLATF TVNILNYVQDNLSWSLGFGIPC++MV AL+VF LGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 S----------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIV 300
                                  +     SSEIA EEE RGLLPHH+SQQFRFL+KALIV
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 301 HNGSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPG 360
            N SKE  H C++ EVEEAKAVLRL PIWVTCLAYA+VFSQSSTFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTIREVEEAKAVLRLVPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMII 420
           FE+PAA+LQS I  SIV+SLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSM+I
Sbjct: 361 FEVPAASLQSVIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AALVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AALVEIKRL+TAQEYGLVDLPKATVPL++WWLVPQYVLFGVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGL 534
           GLRSIGLSLYLSIFGVGNFLSSFLISAIE+L+ GDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

BLAST of Lag0039784 vs. NCBI nr
Match: XP_023535815.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 918.3 bits (2372), Expect = 3.1e-263
Identity = 467/555 (84.14%), Postives = 497/555 (89.55%), Query Frame = 0

Query: 1   MEAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           MEAPLLDETV+GAVDYNGRPV RS  GGWRSAS IIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEAPLLDETVEGAVDYNGRPVLRSKVGGWRSASFIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LRQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATL 120
           L QSTATAAENVN WSGVASLLPLLGAFLADSF+GRYRTIA+SSVLYILGLGLLT+SATL
Sbjct: 61  LGQSTATAAENVNVWSGVASLLPLLGAFLADSFIGRYRTIALSSVLYILGLGLLTVSATL 120

Query: 121 PSPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S CQQ+EKS+ CSP L QV+LFF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 SKAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTY 240
           SKAKSSFFNWWYFCISLATF TVNILNYVQDNLSWSLGFGIPC++MV AL+VF LGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 S----------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIV 300
                                  +     SSEIA EEE RGLLPHH+SQQFRFL+KALIV
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 301 HNGSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPG 360
            N SKE  H C++ EVEEAKAVLRL PIWVTCLAYA+VFSQSSTFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTIREVEEAKAVLRLVPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMII 420
           FE+PAA+LQS I  SIV+SLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSM+I
Sbjct: 361 FEVPAASLQSVIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AALVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AALVEIKRL+TAQEYGLVDLPKATVPL++WWLVPQYVLFGVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGL 534
           GLRSIGLSLYLSIFGVGNFLSSFLISAIE+L+ GDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

BLAST of Lag0039784 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 4.8e-198
Identity = 361/554 (65.16%), Postives = 425/554 (76.71%), Query Frame = 0

Query: 4   PLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLRQ 63
           PLL  T    VDY  +P  +S+SGGWRSA  IIGVEVAERFAYYG+SSNLI +LT  L Q
Sbjct: 13  PLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQ 72

Query: 64  STATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATLPSP 123
           STA AA NVNAWSG ASLLPLLGAF+ADSFLGR+RTI  +S LYI+GLG+LTLSA +P  
Sbjct: 73  STAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIP-- 132

Query: 124 STSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEESKA 183
             SDC+ S    +CSP   QV+ FF +LYLVA AQGGHKPCVQAFGADQFD + PEE KA
Sbjct: 133 --SDCKVSNLLSSCSPRF-QVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKA 192

Query: 184 KSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTYS-- 243
           KSSFFNWWYF +   T TT+ +LNY+QDNLSW+LGFGIPC+AMV AL+V  LGT TY   
Sbjct: 193 KSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFS 252

Query: 244 --------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIVHNG 303
                               K  +  + ++A  EE  GL+   +SQQF FL+KAL+  NG
Sbjct: 253 IRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKNG 312

Query: 304 SKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPGFEI 363
           S      CS+ E+EEAK+VLRL PIW+TCL YA+VF+QS TFFTKQG TM+RSI PG++I
Sbjct: 313 S------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKI 372

Query: 364 PAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMIIAAL 423
             A LQSFIS SIVI +PIYDR+LIPIAR+FT KP GITMLQRIG G+ LS L+M++AAL
Sbjct: 373 SPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAAL 432

Query: 424 VEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGLR 483
           VE+KRLKTA +YGLVD P ATVP++VWWLVPQYVLFG+ DVF MVGLQEFFYDQVP+ LR
Sbjct: 433 VEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELR 492

Query: 484 SIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGLSAI 536
           S+GL+LYLSIFG+GNFLSSF+IS IE+ T   G+ SWF NNLN+AHLDYFYWLLA LS I
Sbjct: 493 SVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFI 551

BLAST of Lag0039784 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 579.7 bits (1493), Expect = 3.5e-164
Identity = 304/552 (55.07%), Postives = 399/552 (72.28%), Query Frame = 0

Query: 2   EAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 61
           E  L +E V  AVD+ G   RRSN+G WR+A  IIGVEVAERFAYYG+ SNLI++LT  L
Sbjct: 6   EISLQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPL 65

Query: 62  RQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATLP 121
            +STA AA NVNAWSG+A+LLP+LGAF+AD+FLGRYRTI ISS++Y+LGL  LTLSA L 
Sbjct: 66  GESTAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFL- 125

Query: 122 SPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEES 181
            P+T++       V  S S    VLFFFSLYLVA  Q GHKPCVQAFGADQFD +  +E 
Sbjct: 126 IPNTTE-------VTSSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEK 185

Query: 182 KAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTR--T 241
             +SSFFNWWY  +S      + ++ Y+Q+  SW+ GFGIPCV MV +L++F  G R   
Sbjct: 186 SDRSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYR 245

Query: 242 YSKK-----------------LACKSSEIAREEETRGLLPHHNS-QQFRFLDKALIVHNG 301
           YSK+                 +A K+  ++  +  +  L  + S ++  F +KAL+V N 
Sbjct: 246 YSKRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVELEANTSPEKQSFFNKALLVPND 305

Query: 302 SKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPGFEI 361
           S +  +    S+VE+A A++RL P+W T LAYAI ++Q  TFFTKQGVTMDR+I+PG +I
Sbjct: 306 SSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKI 365

Query: 362 PAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMIIAAL 421
           P A+LQ FI  SIV+ +PIYDR+ +PIAR  T +P GIT L+RIG G++LS ++M+IAAL
Sbjct: 366 PPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAAL 425

Query: 422 VEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGLR 481
           VE KRL+TA+E+GL+D P+AT+P+++WWL+PQY+L G+ADV+T+VG+QEFFY QVP+ LR
Sbjct: 426 VEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELR 485

Query: 482 SIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGLSAI 534
           SIGL+LYLS  GVG+ LSS LIS I+  TGGD   SWF++NLN+AHLDYFYWLLA +SA+
Sbjct: 486 SIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAV 545

BLAST of Lag0039784 vs. ExPASy Swiss-Prot
Match: Q9SK96 (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 4.5e-164
Identity = 300/553 (54.25%), Postives = 395/553 (71.43%), Query Frame = 0

Query: 2   EAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 61
           EA L++++V  +VD+ G P  +S++GGWRSA  IIGVEV ERFAY+G+ SNLI +LT  L
Sbjct: 7   EAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPL 66

Query: 62  RQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATLP 121
            QSTATAA NVN WSG AS+LP+LGAF+AD++LGRYRTI ++S++YILGLGLLTLS+ L 
Sbjct: 67  GQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSILI 126

Query: 122 SPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEES 181
               S+ +Q  ++ +  P     +LFF SLYLVA  QGGHKPCVQAFGADQFD   P+E 
Sbjct: 127 LMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPKER 186

Query: 182 KAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTYS 241
            ++ SFFNWW+  +S     ++ ++ YVQDN++W+LGFGIPC+ MV AL +F  G +TY 
Sbjct: 187 ISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKTYR 246

Query: 242 KKLA---CKSSEIAR-------EEETRGLLPHHNSQ-----------QFRFLDKALIVHN 301
                   K++  AR         + R L   H+ Q           Q  FL KAL+   
Sbjct: 247 YPRGDREGKNNAFARIGRVFLVAFKNRKLKLTHSGQLEVGSYKKCKGQLEFLAKALLPGE 306

Query: 302 GSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPGFE 361
           G  E    CS  +VE+A A++RL PIW+T +   I ++Q +TFFTKQGVT+DR I+PGFE
Sbjct: 307 GGVEP---CSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKILPGFE 366

Query: 362 IPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMIIAA 421
           IP A+ Q+ I  SI IS+P Y+R+ +P+AR  T KPSGITMLQRIG GM+LS+L+M++AA
Sbjct: 367 IPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLNMVVAA 426

Query: 422 LVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGL 481
           LVE+KRL+TA+E+GLVD P AT+P+++WW VPQY+L G+ DVF++VG QEFFYDQVP+ L
Sbjct: 427 LVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQVPTEL 486

Query: 482 RSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGLSA 534
           RSIGL+L LS  G+ +FLS FLI+ I   TG +G +SWF+ NLN+AH+DYFYWLLA  +A
Sbjct: 487 RSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLLAAFTA 546

BLAST of Lag0039784 vs. ExPASy Swiss-Prot
Match: Q0WSZ6 (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 574.7 bits (1480), Expect = 1.1e-162
Identity = 299/553 (54.07%), Postives = 388/553 (70.16%), Query Frame = 0

Query: 5   LLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLRQS 64
           L +E V  AVD+ G   RRS +G WR+A  IIGVEVAERFA YG+ SNLI++LT  L QS
Sbjct: 9   LQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQS 68

Query: 65  TATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATLPSPS 124
           TA AA NVNAWSG++++LPLLGAF+AD+FLGRY TI I+S +Y+LGL  LTLSA L   +
Sbjct: 69  TAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIPNN 128

Query: 125 TSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEESKAK 184
           T         V  SPS     LFFFSLYLVA  Q GHKPCVQAFGADQFD ++P+E+  +
Sbjct: 129 T--------EVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDR 188

Query: 185 SSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTYS--- 244
           SSFFNWWY  +       + ++ Y+Q+N+SW+LGFGIPCV MV +L++F LG ++Y    
Sbjct: 189 SSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSK 248

Query: 245 ---------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIVHN 304
                                K     SS++ + E         + ++  FL+KAL+V N
Sbjct: 249 TRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALLVPN 308

Query: 305 GSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPGFE 364
            S E    C   +VE+A A++RL P+W+T LAYAI F+Q  TFFTKQGVTM+R+I PG E
Sbjct: 309 DSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVE 368

Query: 365 IPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMIIAA 424
           IP A+LQ  IS SIV+ +PIYDR+L+PI R+ T  P GIT L+RIG GM+L+ L+M++AA
Sbjct: 369 IPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAA 428

Query: 425 LVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGL 484
           LVE KRL+TA+EYGL+D PK T+P+++WWL PQY+L G+ADV T+VG+QEFFY QVP+ L
Sbjct: 429 LVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTEL 488

Query: 485 RSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGLSA 534
           RS+GL++YLS  GVG+ LSS LI  I+  TGGD   SWF++NLN+AHLDYFYWLLA +SA
Sbjct: 489 RSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSA 548

BLAST of Lag0039784 vs. ExPASy Swiss-Prot
Match: Q9C7U1 (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 3.2e-162
Identity = 300/549 (54.64%), Postives = 391/549 (71.22%), Query Frame = 0

Query: 7   DETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLRQSTA 66
           +E V+ +VD+ G P  RS+SG W+S+   +  EVAE+FAY+G++SNLI + T+ L +STA
Sbjct: 16  NENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTA 75

Query: 67  TAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATLPSPSTS 126
            AA NVN W G A+ LPL+   +ADSFLGR+RTI ++S  YI+GLGLLT SAT+PS    
Sbjct: 76  VAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIPSL--- 135

Query: 127 DCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEESKAKSS 186
            C   E   +C  S V+V++FF +LYL+A  +GG K C++AFGADQFD Q P ESKAKSS
Sbjct: 136 -CNDQETRESC-VSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSS 195

Query: 187 FFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTY------ 246
           +FNW YF IS+   TT  + NYVQ+NLSW+LG+ IPC++M+ AL +F LG +TY      
Sbjct: 196 YFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGG 255

Query: 247 ----SKK------------LACKSSEIAREEETRGLLPHHNSQQFRFLDKALIVHNGSKE 306
                KK            +A   +      +T  LLP+ ++++FRFLD+A+I       
Sbjct: 256 EGRQGKKHDNPFVRIGRVFVAAARNRRQTPSDTCLLLPNESTKKFRFLDRAVI------- 315

Query: 307 ESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPGFEIPAA 366
               C   EVEEAKAVL L PIW+  L + IVF+QS TFFTKQG TMDRSI    ++PAA
Sbjct: 316 ---SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISSTLQVPAA 375

Query: 367 ALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMIIAALVEI 426
            LQ FIS +I++ +PIYDR+ +PIAR+ T KP+GIT LQRI  G+ LS +SM+IAALVE+
Sbjct: 376 TLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVIAALVEM 435

Query: 427 KRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGLRSIG 486
           KRLKTA+++GLVD PKATVP++V WL+PQY+LFGV+DVFTMVGLQEFFY +VP  LRS+G
Sbjct: 436 KRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPPQLRSMG 495

Query: 487 LSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGLSAIGLA 534
           L+LYLSI G+GNFLSSF++S IE  T   G+ SWF NNLN+AHLDYFYWLLA LS++   
Sbjct: 496 LALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACLSSLAFI 549

BLAST of Lag0039784 vs. ExPASy TrEMBL
Match: A0A6J1EHW2 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432710 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 6.8e-264
Identity = 468/562 (83.27%), Postives = 504/562 (89.68%), Query Frame = 0

Query: 1   MEAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           MEAPLL++TV+ AVDYNGRPVRRS+SGGWRSA+LIIGVEVAERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVECAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LRQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATL 120
           L QSTATAAENVNAWSG ASLLPLLGAFLADSFLGRYRTIA+S+VLYILGLGLLT+SATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S CQQ+EKS+ CSP L QV+LFFFSLY+VAFAQGGHKPCVQAFGADQFD QHPEE
Sbjct: 121 HSPSISGCQQTEKSLPCSPGLFQVILFFFSLYVVAFAQGGHKPCVQAFGADQFDAQHPEE 180

Query: 181 SKAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTY 240
           SKAKSSFFNWWYFCISLATF TVNILNYVQDNLSWSLGFGIPC++MV AL+VF LGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 S----------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIV 300
                                  +     SSEIA EEE RGLLPHH+SQQFRFL+KAL+V
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKALLV 300

Query: 301 HNGSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPG 360
            N SKE  H C++SEVEEAKAVLRL PIWVTCLAYAIVFSQSSTFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMII 420
           F++PAA+LQSFIS SIV+SLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSM+I
Sbjct: 361 FDVPAASLQSFISLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AALVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AALVEIKRL+TAQEYGLVDLPKATVPL++WWLVPQYVLFGVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRSIGLSLYLSIFGVGNFLSSFLISAIE L+ GDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

BLAST of Lag0039784 vs. ExPASy TrEMBL
Match: A0A6J1IG54 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111472583 PE=3 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.8e-263
Identity = 467/555 (84.14%), Postives = 501/555 (90.27%), Query Frame = 0

Query: 1   MEAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           MEAPLL++TV+GAVDYNGRPVRRS+SGGWRSA+LIIGVEVAERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LRQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATL 120
           L QSTATAAENVNAWSG ASLLPLLGAFLADSFLGRYRTIA+S+VLYILGLGLLT+SATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SP  S CQQ+EKS+ CSP L QV+LFF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPRISGCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 SKAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTY 240
           SKAKSSFFNWWYFCISLATF TVNILNYVQDNLSWSLGFGIPC++MV AL+VF LGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 S----------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIV 300
                                  +     SSEIA EEE RGLLPHH+SQQFRFL+KALIV
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 301 HNGSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPG 360
            N SKE  H CS+SEVEEAKAVLRL PIWVTCLAYAIVFSQSSTFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCSISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMII 420
           F++PAA+LQSFIS SIV+SL IYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSM+I
Sbjct: 361 FDVPAASLQSFISLSIVVSLLIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AALVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AALVEIKRL+TAQEYGLVDLPKATVPL++WWLVPQYVLFGVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGL 534
           GLRSIGLSLYLSIFGVGNFLSSFLISAIE+L+ GDG+ESWF++NLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNSNLNKAHLDYFYWLLAGL 540

BLAST of Lag0039784 vs. ExPASy TrEMBL
Match: A0A6J1EC56 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432731 PE=3 SV=1)

HSP 1 Score: 908.3 bits (2346), Expect = 1.6e-260
Identity = 462/555 (83.24%), Postives = 496/555 (89.37%), Query Frame = 0

Query: 1   MEAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           MEAPLL++TV+GAVDY+GRPVRRS+SGGWRSA+LIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYHGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LRQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATL 120
           L QSTATAAENVN WS  ASLLPLLGAFLADSFLGRYRTIA+SSVLYILGLGLLT+SATL
Sbjct: 61  LGQSTATAAENVNVWSAAASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 120

Query: 121 PSPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S CQQ+EKS+ CSP L QV+LFF S+Y+VAFAQGGHKPCVQAFGADQFD QHPEE
Sbjct: 121 HSPSISGCQQTEKSLPCSPGLFQVILFFSSVYIVAFAQGGHKPCVQAFGADQFDAQHPEE 180

Query: 181 SKAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTY 240
           SKAKSSFFNWWYFCISLATF TVNILNYVQDNLSWSLGFGIPC++MV AL+VF LGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 S----------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIV 300
                                  +     SSEIA EEE  GLLPHH+SQQFRFL+KALIV
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEICGLLPHHSSQQFRFLNKALIV 300

Query: 301 HNGSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPG 360
              SKE  H C++SEVEEAKAVLRL PIWVTCLAYAIVFSQSSTFFTKQGVTM+RSIVPG
Sbjct: 301 PYSSKEGGHTCTISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMII 420
           F++PAA+LQSFI  SIV+SLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSM+I
Sbjct: 361 FDVPAASLQSFIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AALVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AALVEIKRL+TAQEYGLVDLPKATVPL++WWLVPQYVLFGVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGL 534
           GLRSIGLSLYLSIFGVGNFLSSFLISAIE L+ GDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

BLAST of Lag0039784 vs. ExPASy TrEMBL
Match: A0A1S3ATI3 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103482564 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.7e-254
Identity = 455/555 (81.98%), Postives = 487/555 (87.75%), Query Frame = 0

Query: 1   MEAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           ME PLLDETV+GAVDYNG PV RSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LRQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATL 120
           L+QST TAA+NVNAWSG ASLLPLLGAFLADSFLGRY TI +SS LYILGLGLLT+SA +
Sbjct: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILGLGLLTMSALI 120

Query: 121 PSPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPSTS CQQ+EKS+ CSP+L QV+LFFFSLYLV  AQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 SSPSTSACQQTEKSLPCSPNLFQVILFFFSLYLVGLAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 SKAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTY 240
           S +KSSFFNWWYF ISLATF TVNILNYVQDNLSWSLGFGIPC+AMV AL+VFFLGTRTY
Sbjct: 181 SISKSSFFNWWYFGISLATFATVNILNYVQDNLSWSLGFGIPCIAMVLALVVFFLGTRTY 240

Query: 241 S----------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIV 300
                                  K     SSE+AREEE RGLLPHH+S+Q RFLDKALI 
Sbjct: 241 RFSNRGDEENPFVRIGRVFITAVKNWRVNSSEMAREEEIRGLLPHHSSKQLRFLDKALIA 300

Query: 301 HNGSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPG 360
            N  KE+   CS+SEVEEAKAVLRL PIWVTCLA+AIVFSQSSTFFTKQGVTMDRSI  G
Sbjct: 301 PNSLKEDGRACSISEVEEAKAVLRLVPIWVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFG 360

Query: 361 FEIPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMII 420
           FE+PAA+LQSFIS +IVISL IYDRILIPIARNFTGKPSGIT LQRIGFGMLLSA+SM+I
Sbjct: 361 FEVPAASLQSFISLAIVISLMIYDRILIPIARNFTGKPSGITTLQRIGFGMLLSAISMVI 420

Query: 421 AALVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AALVEIKRLKTAQEYGLVDLPKATVPL++WWLVPQYVLFGVAD FTMVGLQEFFYDQVPS
Sbjct: 421 AALVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQVPS 480

Query: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGL 534
           GLRSIGLSLYLSIFG+GNFLSSFLISAIE+LT G+G++SWFDNNLNKAHLDYFYWLL GL
Sbjct: 481 GLRSIGLSLYLSIFGIGNFLSSFLISAIEKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGL 540

BLAST of Lag0039784 vs. ExPASy TrEMBL
Match: A0A5A7TJ76 (Protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00110 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.7e-254
Identity = 455/555 (81.98%), Postives = 487/555 (87.75%), Query Frame = 0

Query: 1   MEAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           ME PLLDETV+GAVDYNG PV RSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LRQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATL 120
           L+QST TAA+NVNAWSG ASLLPLLGAFLADSFLGRY TI +SS LYILGLGLLT+SA +
Sbjct: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILGLGLLTMSALI 120

Query: 121 PSPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPSTS CQQ+EKS+ CSP+L QV+LFFFSLYLV  AQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 SSPSTSACQQTEKSLPCSPNLFQVILFFFSLYLVGLAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 SKAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTY 240
           S +KSSFFNWWYF ISLATF TVNILNYVQDNLSWSLGFGIPC+AMV AL+VFFLGTRTY
Sbjct: 181 SISKSSFFNWWYFGISLATFATVNILNYVQDNLSWSLGFGIPCIAMVLALVVFFLGTRTY 240

Query: 241 S----------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIV 300
                                  K     SSE+AREEE RGLLPHH+S+Q RFLDKALI 
Sbjct: 241 RFSNRGDEENPFVRIGRVFITAVKNWRVNSSEMAREEEIRGLLPHHSSKQLRFLDKALIA 300

Query: 301 HNGSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPG 360
            N  KE+   CS+SEVEEAKAVLRL PIWVTCLA+AIVFSQSSTFFTKQGVTMDRSI  G
Sbjct: 301 PNSLKEDGRACSISEVEEAKAVLRLVPIWVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFG 360

Query: 361 FEIPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMII 420
           FE+PAA+LQSFIS +IVISL IYDRILIPIARNFTGKPSGIT LQRIGFGMLLSA+SM+I
Sbjct: 361 FEVPAASLQSFISLAIVISLMIYDRILIPIARNFTGKPSGITTLQRIGFGMLLSAISMVI 420

Query: 421 AALVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AALVEIKRLKTAQEYGLVDLPKATVPL++WWLVPQYVLFGVAD FTMVGLQEFFYDQVPS
Sbjct: 421 AALVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQVPS 480

Query: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGL 534
           GLRSIGLSLYLSIFG+GNFLSSFLISAIE+LT G+G++SWFDNNLNKAHLDYFYWLL GL
Sbjct: 481 GLRSIGLSLYLSIFGIGNFLSSFLISAIEKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGL 540

BLAST of Lag0039784 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 692.2 bits (1785), Expect = 3.4e-199
Identity = 361/554 (65.16%), Postives = 425/554 (76.71%), Query Frame = 0

Query: 4   PLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLRQ 63
           PLL  T    VDY  +P  +S+SGGWRSA  IIGVEVAERFAYYG+SSNLI +LT  L Q
Sbjct: 13  PLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQ 72

Query: 64  STATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATLPSP 123
           STA AA NVNAWSG ASLLPLLGAF+ADSFLGR+RTI  +S LYI+GLG+LTLSA +P  
Sbjct: 73  STAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIP-- 132

Query: 124 STSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEESKA 183
             SDC+ S    +CSP   QV+ FF +LYLVA AQGGHKPCVQAFGADQFD + PEE KA
Sbjct: 133 --SDCKVSNLLSSCSPRF-QVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKA 192

Query: 184 KSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTYS-- 243
           KSSFFNWWYF +   T TT+ +LNY+QDNLSW+LGFGIPC+AMV AL+V  LGT TY   
Sbjct: 193 KSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFS 252

Query: 244 --------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIVHNG 303
                               K  +  + ++A  EE  GL+   +SQQF FL+KAL+  NG
Sbjct: 253 IRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKNG 312

Query: 304 SKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPGFEI 363
           S      CS+ E+EEAK+VLRL PIW+TCL YA+VF+QS TFFTKQG TM+RSI PG++I
Sbjct: 313 S------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKI 372

Query: 364 PAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMIIAAL 423
             A LQSFIS SIVI +PIYDR+LIPIAR+FT KP GITMLQRIG G+ LS L+M++AAL
Sbjct: 373 SPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAAL 432

Query: 424 VEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGLR 483
           VE+KRLKTA +YGLVD P ATVP++VWWLVPQYVLFG+ DVF MVGLQEFFYDQVP+ LR
Sbjct: 433 VEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELR 492

Query: 484 SIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGLSAI 536
           S+GL+LYLSIFG+GNFLSSF+IS IE+ T   G+ SWF NNLN+AHLDYFYWLLA LS I
Sbjct: 493 SVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFI 551

BLAST of Lag0039784 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 579.7 bits (1493), Expect = 2.5e-165
Identity = 304/552 (55.07%), Postives = 399/552 (72.28%), Query Frame = 0

Query: 2   EAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 61
           E  L +E V  AVD+ G   RRSN+G WR+A  IIGVEVAERFAYYG+ SNLI++LT  L
Sbjct: 6   EISLQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPL 65

Query: 62  RQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATLP 121
            +STA AA NVNAWSG+A+LLP+LGAF+AD+FLGRYRTI ISS++Y+LGL  LTLSA L 
Sbjct: 66  GESTAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFL- 125

Query: 122 SPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEES 181
            P+T++       V  S S    VLFFFSLYLVA  Q GHKPCVQAFGADQFD +  +E 
Sbjct: 126 IPNTTE-------VTSSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEK 185

Query: 182 KAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTR--T 241
             +SSFFNWWY  +S      + ++ Y+Q+  SW+ GFGIPCV MV +L++F  G R   
Sbjct: 186 SDRSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYR 245

Query: 242 YSKK-----------------LACKSSEIAREEETRGLLPHHNS-QQFRFLDKALIVHNG 301
           YSK+                 +A K+  ++  +  +  L  + S ++  F +KAL+V N 
Sbjct: 246 YSKRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVELEANTSPEKQSFFNKALLVPND 305

Query: 302 SKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPGFEI 361
           S +  +    S+VE+A A++RL P+W T LAYAI ++Q  TFFTKQGVTMDR+I+PG +I
Sbjct: 306 SSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKI 365

Query: 362 PAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMIIAAL 421
           P A+LQ FI  SIV+ +PIYDR+ +PIAR  T +P GIT L+RIG G++LS ++M+IAAL
Sbjct: 366 PPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAAL 425

Query: 422 VEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGLR 481
           VE KRL+TA+E+GL+D P+AT+P+++WWL+PQY+L G+ADV+T+VG+QEFFY QVP+ LR
Sbjct: 426 VEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELR 485

Query: 482 SIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGLSAI 534
           SIGL+LYLS  GVG+ LSS LIS I+  TGGD   SWF++NLN+AHLDYFYWLLA +SA+
Sbjct: 486 SIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAV 545

BLAST of Lag0039784 vs. TAIR 10
Match: AT1G22550.1 (Major facilitator superfamily protein )

HSP 1 Score: 579.3 bits (1492), Expect = 3.2e-165
Identity = 300/553 (54.25%), Postives = 395/553 (71.43%), Query Frame = 0

Query: 2   EAPLLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 61
           EA L++++V  +VD+ G P  +S++GGWRSA  IIGVEV ERFAY+G+ SNLI +LT  L
Sbjct: 7   EAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPL 66

Query: 62  RQSTATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATLP 121
            QSTATAA NVN WSG AS+LP+LGAF+AD++LGRYRTI ++S++YILGLGLLTLS+ L 
Sbjct: 67  GQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSILI 126

Query: 122 SPSTSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEES 181
               S+ +Q  ++ +  P     +LFF SLYLVA  QGGHKPCVQAFGADQFD   P+E 
Sbjct: 127 LMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPKER 186

Query: 182 KAKSSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTYS 241
            ++ SFFNWW+  +S     ++ ++ YVQDN++W+LGFGIPC+ MV AL +F  G +TY 
Sbjct: 187 ISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKTYR 246

Query: 242 KKLA---CKSSEIAR-------EEETRGLLPHHNSQ-----------QFRFLDKALIVHN 301
                   K++  AR         + R L   H+ Q           Q  FL KAL+   
Sbjct: 247 YPRGDREGKNNAFARIGRVFLVAFKNRKLKLTHSGQLEVGSYKKCKGQLEFLAKALLPGE 306

Query: 302 GSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPGFE 361
           G  E    CS  +VE+A A++RL PIW+T +   I ++Q +TFFTKQGVT+DR I+PGFE
Sbjct: 307 GGVEP---CSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKILPGFE 366

Query: 362 IPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMIIAA 421
           IP A+ Q+ I  SI IS+P Y+R+ +P+AR  T KPSGITMLQRIG GM+LS+L+M++AA
Sbjct: 367 IPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLNMVVAA 426

Query: 422 LVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGL 481
           LVE+KRL+TA+E+GLVD P AT+P+++WW VPQY+L G+ DVF++VG QEFFYDQVP+ L
Sbjct: 427 LVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQVPTEL 486

Query: 482 RSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGLSA 534
           RSIGL+L LS  G+ +FLS FLI+ I   TG +G +SWF+ NLN+AH+DYFYWLLA  +A
Sbjct: 487 RSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLLAAFTA 546

BLAST of Lag0039784 vs. TAIR 10
Match: AT1G72125.1 (Major facilitator superfamily protein )

HSP 1 Score: 574.7 bits (1480), Expect = 7.9e-164
Identity = 299/553 (54.07%), Postives = 388/553 (70.16%), Query Frame = 0

Query: 5   LLDETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLRQS 64
           L +E V  AVD+ G   RRS +G WR+A  IIGVEVAERFA YG+ SNLI++LT  L QS
Sbjct: 9   LQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQS 68

Query: 65  TATAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATLPSPS 124
           TA AA NVNAWSG++++LPLLGAF+AD+FLGRY TI I+S +Y+LGL  LTLSA L   +
Sbjct: 69  TAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIPNN 128

Query: 125 TSDCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEESKAK 184
           T         V  SPS     LFFFSLYLVA  Q GHKPCVQAFGADQFD ++P+E+  +
Sbjct: 129 T--------EVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDR 188

Query: 185 SSFFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTYS--- 244
           SSFFNWWY  +       + ++ Y+Q+N+SW+LGFGIPCV MV +L++F LG ++Y    
Sbjct: 189 SSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSK 248

Query: 245 ---------------------KKLACKSSEIAREEETRGLLPHHNSQQFRFLDKALIVHN 304
                                K     SS++ + E         + ++  FL+KAL+V N
Sbjct: 249 TRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALLVPN 308

Query: 305 GSKEESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPGFE 364
            S E    C   +VE+A A++RL P+W+T LAYAI F+Q  TFFTKQGVTM+R+I PG E
Sbjct: 309 DSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVE 368

Query: 365 IPAAALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMIIAA 424
           IP A+LQ  IS SIV+ +PIYDR+L+PI R+ T  P GIT L+RIG GM+L+ L+M++AA
Sbjct: 369 IPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAA 428

Query: 425 LVEIKRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGL 484
           LVE KRL+TA+EYGL+D PK T+P+++WWL PQY+L G+ADV T+VG+QEFFY QVP+ L
Sbjct: 429 LVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTEL 488

Query: 485 RSIGLSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGLSA 534
           RS+GL++YLS  GVG+ LSS LI  I+  TGGD   SWF++NLN+AHLDYFYWLLA +SA
Sbjct: 489 RSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSA 548

BLAST of Lag0039784 vs. TAIR 10
Match: AT1G72140.1 (Major facilitator superfamily protein )

HSP 1 Score: 573.2 bits (1476), Expect = 2.3e-163
Identity = 300/549 (54.64%), Postives = 391/549 (71.22%), Query Frame = 0

Query: 7   DETVDGAVDYNGRPVRRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLRQSTA 66
           +E V+ +VD+ G P  RS+SG W+S+   +  EVAE+FAY+G++SNLI + T+ L +STA
Sbjct: 16  NENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTA 75

Query: 67  TAAENVNAWSGVASLLPLLGAFLADSFLGRYRTIAISSVLYILGLGLLTLSATLPSPSTS 126
            AA NVN W G A+ LPL+   +ADSFLGR+RTI ++S  YI+GLGLLT SAT+PS    
Sbjct: 76  VAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIPSL--- 135

Query: 127 DCQQSEKSVACSPSLVQVVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEESKAKSS 186
            C   E   +C  S V+V++FF +LYL+A  +GG K C++AFGADQFD Q P ESKAKSS
Sbjct: 136 -CNDQETRESC-VSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSS 195

Query: 187 FFNWWYFCISLATFTTVNILNYVQDNLSWSLGFGIPCVAMVFALLVFFLGTRTY------ 246
           +FNW YF IS+   TT  + NYVQ+NLSW+LG+ IPC++M+ AL +F LG +TY      
Sbjct: 196 YFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGG 255

Query: 247 ----SKK------------LACKSSEIAREEETRGLLPHHNSQQFRFLDKALIVHNGSKE 306
                KK            +A   +      +T  LLP+ ++++FRFLD+A+I       
Sbjct: 256 EGRQGKKHDNPFVRIGRVFVAAARNRRQTPSDTCLLLPNESTKKFRFLDRAVI------- 315

Query: 307 ESHVCSVSEVEEAKAVLRLGPIWVTCLAYAIVFSQSSTFFTKQGVTMDRSIVPGFEIPAA 366
               C   EVEEAKAVL L PIW+  L + IVF+QS TFFTKQG TMDRSI    ++PAA
Sbjct: 316 ---SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISSTLQVPAA 375

Query: 367 ALQSFISASIVISLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMIIAALVEI 426
            LQ FIS +I++ +PIYDR+ +PIAR+ T KP+GIT LQRI  G+ LS +SM+IAALVE+
Sbjct: 376 TLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVIAALVEM 435

Query: 427 KRLKTAQEYGLVDLPKATVPLTVWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGLRSIG 486
           KRLKTA+++GLVD PKATVP++V WL+PQY+LFGV+DVFTMVGLQEFFY +VP  LRS+G
Sbjct: 436 KRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPPQLRSMG 495

Query: 487 LSLYLSIFGVGNFLSSFLISAIERLTGGDGRESWFDNNLNKAHLDYFYWLLAGLSAIGLA 534
           L+LYLSI G+GNFLSSF++S IE  T   G+ SWF NNLN+AHLDYFYWLLA LS++   
Sbjct: 496 LALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACLSSLAFI 549