Lag0039768 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0039768
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein NRT1/ PTR FAMILY 5.10-like
Locationchr2: 49578396 .. 49580657 (+)
RNA-Seq ExpressionLag0039768
SyntenyLag0039768
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACCGCCGAACTCCGCTGCTGGCCGAAACCGTGGACGGCGCCCTTGATTTTCAAGGTCAGCCAGTCCTCCGATCCTCCTCCGGCGGTTGGAAGTCCGCCTCTTTCATGATTGGTAATACATTTACATTCTCCAACTGTTCCATGTATTTACTTGCTTTCTCATGGTGATGGTGATGCTTGCCAATTTACGATCCATTTAGGGGTGGAGGTCGCCGAGAGATTTGCTTACTACGGAATCGGTTCCAACCTCATAACTTACCTGACCGGCCCACTCGGTCAGTCTGTGGCCACCGCCGCCGAGAGTATTAACATTTGGTCAGGCCTCTCCATGCTACTCACTCTCCTCGGAGCCTTTCTCGCTGATACCTTCTTCGGACGATATCGCACCATTCTTTTTTCTTCTGCAATTTACGTCTTGGTCTGCTCACTAACTTTCTTCCTTGTTTCGTATTCATTTCAATCAGAAATCGAACCATGTCTATCCAAAATGTATCTGTTCAGATTTCTGAGTTTAAGTTCTGATGATTCCTAACAGGGACTAGCCTTGCTCTCTTTCTCTGCTCTGCTTCCTACAACAACCTCTTCCCACTGTCACAACACTTACGAAATTTCATCGTGTTCTTCCACTCCCAAGTTTCAGCTCATGTTGTTCTTCGTCTCTCTGTATCTAATAGGGATAGGGCAAGGTGGGCACAAGCCTTGCGTACAGGCTTTTGGAGCCGATCAGTTCGATGCCCAAAACCCACAAGAGGCCAAATCCAAAAGCTCCTTCTTCAATTGGTGGTTTTTTGGTGTGTGCGCTGGTAGCTTTGTGGCTATCTTGGTGGTAACATATACAGAGGAAAACCTCAGTTGGAGTTTGGGATTTGGAATTCCTTGTATTGTGATGGTGATAGCATCGGTTGTGTTTGTATCTGGGACAAAGACGTATCGTTATAGCGTCAAAAAGTATCATAAGAGCCCATTTGTAAGAATTGGTCGGGTATTTGTGTCTGCAGTTAGGAATTGGAGAGCTTCTTCAACAGTCAACTTTGATGAGGAAGAAGGTGGTAGAGATTTGTCTCAACAGAATGCTGGACAATTCAGGTAAGAATATTACAAAATTCTTGCCGCATCCGACGAAAATGGGGTTTAGAGTAATTATAAACTTCACGTTATACTTCTATTTTCTCTTATTGTCAATGCAATTGTTGAGATGCTAACATGAGTCAGAGTTCATAATGCAAGTACAGCACTAAATTTATGATTTCGATAATAAGAGTTTTCATCGATTTATTCAAGAACTTCACTTACTCAACTAATAGGTTTACCAGGAACCTGAAGTGGGTGGAATCGAGTGCAGTTCAATCAAATTGGAAATTTGCTGATTTATAAAGTTGAAATATATAATATATACAGTTCAATTAAATTTAACCGACATTTCTAAACAACTATTGGTTCCTCAAATGTGGCAGATTTCTTAATAAAGCTTGCATTGTCCCAAATCATTCCAACCCATGCAGAATGGTGTGCAGCGTCAGCGAGGTCGAAGAAGCAAAGGCAGTTCTTAGGATCTTTCCAGTATGGATGACAGTTCTTGTATTTGGTATTGTGTTTGGACAAGATTCCACATTCTTTACCAAGCAAGGAGCTACAATGGATCGATCAATTTTATCTGGCTTTATCATTCCTGCTGCTGCACTGGATTCATTCGTTCCTCTTTCTATTGTTATCTTTATCACCATCTATGATCTTGTATTTGTTCCTGTAGCCAGAGCTTTTACAGGAATACAGTCTGGCATCACAACACTGCAGCGAATAGGAACTGGGCTGATTGTATCTGCTATTTCAATGTTGGTTGCGGCTATGGTTGAGAGGAAAAGATTAAGAATTGCAGAAGAACACGGTCTAGTTGATAGGCCTGACATAACCATTCCAATGAGCTTTTGGTGGCTAGTTCCTCAATATACCTTGTTTGGTTTGGCTGAAGCTTTTACATTGGTTGGTCTTCAAGAGTTTTTCTACGATCAATGTCCAACAGATTTGAAAAGCATGGGGCTTGCCTTTTATACTAGCGTCCTAGGCATGGGAAGCATTTTAAGCAGTCTTTTAGTTTCTGTTATTGATGAAGCAACTGGAACCAATGGGCAGAATAGTTGGTTTTCCAACAATCTGAACAAGGCGCATCTTGATTACTTTTATTTGCTATTGTCTGGGCTCAGTGTTCTCGCGTTTGTTGCCTTCCTATTTGTTTCCAACTCGCATGTTCATAGCAGCTGA

mRNA sequence

ATGGACCGCCGAACTCCGCTGCTGGCCGAAACCGTGGACGGCGCCCTTGATTTTCAAGGTCAGCCAGTCCTCCGATCCTCCTCCGGCGGTTGGAAGTCCGCCTCTTTCATGATTGGGGTGGAGGTCGCCGAGAGATTTGCTTACTACGGAATCGGTTCCAACCTCATAACTTACCTGACCGGCCCACTCGGTCAGTCTGTGGCCACCGCCGCCGAGAGTATTAACATTTGGTCAGGCCTCTCCATGCTACTCACTCTCCTCGGAGCCTTTCTCGCTGATACCTTCTTCGGACGATATCGCACCATTCTTTTTTCTTCTGCAATTTACGTCTTGGGACTAGCCTTGCTCTCTTTCTCTGCTCTGCTTCCTACAACAACCTCTTCCCACTGTCACAACACTTACGAAATTTCATCGTGTTCTTCCACTCCCAAGTTTCAGCTCATGTTGTTCTTCGTCTCTCTGTATCTAATAGGGATAGGGCAAGGTGGGCACAAGCCTTGCGTACAGGCTTTTGGAGCCGATCAGTTCGATGCCCAAAACCCACAAGAGGCCAAATCCAAAAGCTCCTTCTTCAATTGGTGGTTTTTTGGTGTGTGCGCTGGTAGCTTTGTGGCTATCTTGGTGGTAACATATACAGAGGAAAACCTCAGTTGGAGTTTGGGATTTGGAATTCCTTGTATTGTGATGGTGATAGCATCGGTTGTGTTTGTATCTGGGACAAAGACGTATCGTTATAGCGTCAAAAAGTATCATAAGAGCCCATTTGTAAGAATTGGTCGGGTATTTGTGTCTGCAGTTAGGAATTGGAGAGCTTCTTCAACAGTCAACTTTGATGAGGAAGAAGGTGGTAGAGATTTGTCTCAACAGAATGCTGGACAATTCAGATTTCTTAATAAAGCTTGCATTGTCCCAAATCATTCCAACCCATGCAGAATGGTGTGCAGCGTCAGCGAGGTCGAAGAAGCAAAGGCAGTTCTTAGGATCTTTCCAGTATGGATGACAGTTCTTGTATTTGGTATTGTGTTTGGACAAGATTCCACATTCTTTACCAAGCAAGGAGCTACAATGGATCGATCAATTTTATCTGGCTTTATCATTCCTGCTGCTGCACTGGATTCATTCGTTCCTCTTTCTATTGTTATCTTTATCACCATCTATGATCTTGTATTTGTTCCTGTAGCCAGAGCTTTTACAGGAATACAGTCTGGCATCACAACACTGCAGCGAATAGGAACTGGGCTGATTGTATCTGCTATTTCAATGTTGGTTGCGGCTATGGTTGAGAGGAAAAGATTAAGAATTGCAGAAGAACACGGTCTAGTTGATAGGCCTGACATAACCATTCCAATGAGCTTTTGGTGGCTAGTTCCTCAATATACCTTGTTTGGTTTGGCTGAAGCTTTTACATTGGTTGGTCTTCAAGAGTTTTTCTACGATCAATGTCCAACAGATTTGAAAAGCATGGGGCTTGCCTTTTATACTAGCGTCCTAGGCATGGGAAGCATTTTAAGCAGTCTTTTAGTTTCTGTTATTGATGAAGCAACTGGAACCAATGGGCAGAATAGTTGGTTTTCCAACAATCTGAACAAGGCGCATCTTGATTACTTTTATTTGCTATTGTCTGGGCTCAGTGTTCTCGCGTTTGTTGCCTTCCTATTTGTTTCCAACTCGCATGTTCATAGCAGCTGA

Coding sequence (CDS)

ATGGACCGCCGAACTCCGCTGCTGGCCGAAACCGTGGACGGCGCCCTTGATTTTCAAGGTCAGCCAGTCCTCCGATCCTCCTCCGGCGGTTGGAAGTCCGCCTCTTTCATGATTGGGGTGGAGGTCGCCGAGAGATTTGCTTACTACGGAATCGGTTCCAACCTCATAACTTACCTGACCGGCCCACTCGGTCAGTCTGTGGCCACCGCCGCCGAGAGTATTAACATTTGGTCAGGCCTCTCCATGCTACTCACTCTCCTCGGAGCCTTTCTCGCTGATACCTTCTTCGGACGATATCGCACCATTCTTTTTTCTTCTGCAATTTACGTCTTGGGACTAGCCTTGCTCTCTTTCTCTGCTCTGCTTCCTACAACAACCTCTTCCCACTGTCACAACACTTACGAAATTTCATCGTGTTCTTCCACTCCCAAGTTTCAGCTCATGTTGTTCTTCGTCTCTCTGTATCTAATAGGGATAGGGCAAGGTGGGCACAAGCCTTGCGTACAGGCTTTTGGAGCCGATCAGTTCGATGCCCAAAACCCACAAGAGGCCAAATCCAAAAGCTCCTTCTTCAATTGGTGGTTTTTTGGTGTGTGCGCTGGTAGCTTTGTGGCTATCTTGGTGGTAACATATACAGAGGAAAACCTCAGTTGGAGTTTGGGATTTGGAATTCCTTGTATTGTGATGGTGATAGCATCGGTTGTGTTTGTATCTGGGACAAAGACGTATCGTTATAGCGTCAAAAAGTATCATAAGAGCCCATTTGTAAGAATTGGTCGGGTATTTGTGTCTGCAGTTAGGAATTGGAGAGCTTCTTCAACAGTCAACTTTGATGAGGAAGAAGGTGGTAGAGATTTGTCTCAACAGAATGCTGGACAATTCAGATTTCTTAATAAAGCTTGCATTGTCCCAAATCATTCCAACCCATGCAGAATGGTGTGCAGCGTCAGCGAGGTCGAAGAAGCAAAGGCAGTTCTTAGGATCTTTCCAGTATGGATGACAGTTCTTGTATTTGGTATTGTGTTTGGACAAGATTCCACATTCTTTACCAAGCAAGGAGCTACAATGGATCGATCAATTTTATCTGGCTTTATCATTCCTGCTGCTGCACTGGATTCATTCGTTCCTCTTTCTATTGTTATCTTTATCACCATCTATGATCTTGTATTTGTTCCTGTAGCCAGAGCTTTTACAGGAATACAGTCTGGCATCACAACACTGCAGCGAATAGGAACTGGGCTGATTGTATCTGCTATTTCAATGTTGGTTGCGGCTATGGTTGAGAGGAAAAGATTAAGAATTGCAGAAGAACACGGTCTAGTTGATAGGCCTGACATAACCATTCCAATGAGCTTTTGGTGGCTAGTTCCTCAATATACCTTGTTTGGTTTGGCTGAAGCTTTTACATTGGTTGGTCTTCAAGAGTTTTTCTACGATCAATGTCCAACAGATTTGAAAAGCATGGGGCTTGCCTTTTATACTAGCGTCCTAGGCATGGGAAGCATTTTAAGCAGTCTTTTAGTTTCTGTTATTGATGAAGCAACTGGAACCAATGGGCAGAATAGTTGGTTTTCCAACAATCTGAACAAGGCGCATCTTGATTACTTTTATTTGCTATTGTCTGGGCTCAGTGTTCTCGCGTTTGTTGCCTTCCTATTTGTTTCCAACTCGCATGTTCATAGCAGCTGA

Protein sequence

MDRRTPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPLGQSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLPTTTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQEAKSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTYRYSVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKACIVPNHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSILSGFIIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISMLVAAMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQCPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLSGLSVLAFVAFLFVSNSHVHSS
Homology
BLAST of Lag0039768 vs. NCBI nr
Match: XP_022954701.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita moschata] >XP_022954702.1 protein NRT1/ PTR FAMILY 5.10-like [Cucurbita moschata])

HSP 1 Score: 971.5 bits (2510), Expect = 3.2e-279
Identity = 497/557 (89.23%), Postives = 525/557 (94.25%), Query Frame = 0

Query: 5   TPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64
           +PLL +TVDGA+DFQG PVLRSSSGGW+SASFMIGVEVAERFAYYGIG+NLITYLTGPLG
Sbjct: 10  SPLLNDTVDGAVDFQGHPVLRSSSGGWRSASFMIGVEVAERFAYYGIGANLITYLTGPLG 69

Query: 65  QSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLPT 124
           QSVATAAES+NIWSG+SML TLLGAF+AD+FFGRYRTILFSSAIYVLGLALLSFSALLPT
Sbjct: 70  QSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLALLSFSALLPT 129

Query: 125 TTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQEA 184
           TTS  C N+ EISSC STP+FQLMLFFVSLYLIG+GQGGHKPCVQAFGADQFDAQ+PQEA
Sbjct: 130 TTSLLCQNSNEISSC-STPQFQLMLFFVSLYLIGVGQGGHKPCVQAFGADQFDAQHPQEA 189

Query: 185 KSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTYR 244
           KSKSSFFNWWFFGVCAG+FVAILVVTYTEENLSWSLGFGIP I+MVIASVVFV G  TYR
Sbjct: 190 KSKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVFVFGKNTYR 249

Query: 245 YSVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKACIVP 304
           YSVKKY KSPFVRIGRVFVSA RNWRASST  F +EE   DLSQQ AGQFRFLNKACIVP
Sbjct: 250 YSVKKYDKSPFVRIGRVFVSAARNWRASSTFIFGQEESAIDLSQQKAGQFRFLNKACIVP 309

Query: 305 NHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSILSGF 364
           NHSN C MVCS+SEVEEAKAVLRIFPVWMTVLVFGIVF QDSTFFTKQGATMDRSILSGF
Sbjct: 310 NHSNLCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRSILSGF 369

Query: 365 IIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISMLVA 424
           IIPAAA+DSFVPLSIVIFITIYD VFV +ARAFTG+QSGITTLQRIGTGL++SAISMLVA
Sbjct: 370 IIPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGVQSGITTLQRIGTGLVISAISMLVA 429

Query: 425 AMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQCPTD 484
           +MVERKRLRIAEE GLVDRPDITIPMSFWWLVPQYTL+GLAE FTLVGLQEFFYDQCP+D
Sbjct: 430 SMVERKRLRIAEEQGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQEFFYDQCPSD 489

Query: 485 LKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLSGLS 544
           LKSMGLAFYTSVLGMGSILSSLLVSVIDEATG NGQNSWFS+NLNKAHLDYFYLLLSGLS
Sbjct: 490 LKSMGLAFYTSVLGMGSILSSLLVSVIDEATGANGQNSWFSDNLNKAHLDYFYLLLSGLS 549

Query: 545 VLAFVAFLFVSNSHVHS 562
           +LAFV FLFVS  HV+S
Sbjct: 550 LLAFVGFLFVSTWHVYS 565

BLAST of Lag0039768 vs. NCBI nr
Match: XP_023541977.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 969.9 bits (2506), Expect = 9.3e-279
Identity = 497/557 (89.23%), Postives = 524/557 (94.08%), Query Frame = 0

Query: 5   TPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64
           +PLL +TVDGA+DFQG PVLRSSSGGW+SASFMIGVEVAERFAYYGIG+NLITYLTGPLG
Sbjct: 50  SPLLNDTVDGAVDFQGHPVLRSSSGGWRSASFMIGVEVAERFAYYGIGANLITYLTGPLG 109

Query: 65  QSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLPT 124
           QSVATAAES+NIWSG+SML TLLGAF+AD+FFGRYRTILFSSAIYVLGLALLSFSALLPT
Sbjct: 110 QSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLALLSFSALLPT 169

Query: 125 TTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQEA 184
           TTS  C N+ EISSC ST +FQL+LFFVSLYLIG+GQGGHKPCVQAFGADQFDAQ+PQEA
Sbjct: 170 TTSLLCQNSNEISSC-STAQFQLVLFFVSLYLIGVGQGGHKPCVQAFGADQFDAQHPQEA 229

Query: 185 KSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTYR 244
           KSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIP I+MVIASVVFV G  TYR
Sbjct: 230 KSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVFVFGKNTYR 289

Query: 245 YSVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKACIVP 304
           YSVKKY KSPFVRIGRVFVSA RNWRASST  F +EE   DLSQQ AGQFRFLNKACIVP
Sbjct: 290 YSVKKYDKSPFVRIGRVFVSAARNWRASSTFIFYQEESATDLSQQKAGQFRFLNKACIVP 349

Query: 305 NHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSILSGF 364
           NHSN C MVCS+SEVEEAKAVLRIFPVWMTVLVFGIVF QDSTFFTKQGATMDRSILSGF
Sbjct: 350 NHSNQCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRSILSGF 409

Query: 365 IIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISMLVA 424
           I PAAA+DSFVPLSIVIFITIYD VFV +ARAFTGIQSGITTLQRIGTGL++SAISMLVA
Sbjct: 410 ITPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGIQSGITTLQRIGTGLVISAISMLVA 469

Query: 425 AMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQCPTD 484
           +MVERKRLRIAEE GLVDRPDITIPMSFWWLVPQYTL+GLAE FTLVGLQEFFYDQCP+D
Sbjct: 470 SMVERKRLRIAEEQGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQEFFYDQCPSD 529

Query: 485 LKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLSGLS 544
           LKSMGLAFYTSVLGMGSILSSLLVSVIDEATG NGQNSWFS+NLNKAHLDYFYLLLSGLS
Sbjct: 530 LKSMGLAFYTSVLGMGSILSSLLVSVIDEATGANGQNSWFSDNLNKAHLDYFYLLLSGLS 589

Query: 545 VLAFVAFLFVSNSHVHS 562
           +LAFV FLFVS SHV+S
Sbjct: 590 LLAFVGFLFVSTSHVYS 605

BLAST of Lag0039768 vs. NCBI nr
Match: XP_022994214.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita maxima])

HSP 1 Score: 959.1 bits (2478), Expect = 1.6e-275
Identity = 490/549 (89.25%), Postives = 518/549 (94.35%), Query Frame = 0

Query: 5   TPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64
           +PLL +TVDG +DFQG PVLRSS+GGW+SASFMIGVEVAERFAYYGIG+NLITYLTGPLG
Sbjct: 10  SPLLNDTVDGTVDFQGHPVLRSSAGGWRSASFMIGVEVAERFAYYGIGANLITYLTGPLG 69

Query: 65  QSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLPT 124
           QSVATAAES+NIWSG+SML TLLGAF+AD+FFGRYRTILFSSAIYVLGLALLSFSALLPT
Sbjct: 70  QSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLALLSFSALLPT 129

Query: 125 TTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQEA 184
           TTS  C N+ EISSC STP+FQL+LFFVSLYLIG+GQGGHKPCVQAFGADQFDAQ+PQEA
Sbjct: 130 TTSLLCQNSNEISSC-STPQFQLVLFFVSLYLIGVGQGGHKPCVQAFGADQFDAQHPQEA 189

Query: 185 KSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTYR 244
           KSKSSFFNWWFFGVCAG+FVAILVVTYTEENLSWSLGFGIP I+MVIASVVFV G  TYR
Sbjct: 190 KSKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVFVFGKYTYR 249

Query: 245 YSVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKACIVP 304
           YSVKKY KSPFVRIGRVFVSA RNWRASST  FD+EE   DLSQQ AGQFRFLNKACIVP
Sbjct: 250 YSVKKYDKSPFVRIGRVFVSAARNWRASSTFIFDQEESATDLSQQKAGQFRFLNKACIVP 309

Query: 305 NHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSILSGF 364
           NHSN C MVCS+SEVEEAKAVLRIFPVWMTVLVFGIVF QDSTFFTKQGATMDRSILSGF
Sbjct: 310 NHSNQCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRSILSGF 369

Query: 365 IIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISMLVA 424
           IIPAAA+DSFVPLSIVIFITIYD VFV +ARAFTGIQSGITTLQRIGTGL++SAISMLVA
Sbjct: 370 IIPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGIQSGITTLQRIGTGLVISAISMLVA 429

Query: 425 AMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQCPTD 484
           +MVERKRLRIAEE GLVDRPDITIPMSFWWLVPQYTL+GLAE FTLVGLQEFFYDQCP+D
Sbjct: 430 SMVERKRLRIAEETGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQEFFYDQCPSD 489

Query: 485 LKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLSGLS 544
           LKSMGLAFYTSVLG GSILSSLLVSVIDEATG NGQNSWFS+NLNKAHLDYFYLLLSGLS
Sbjct: 490 LKSMGLAFYTSVLGTGSILSSLLVSVIDEATGANGQNSWFSDNLNKAHLDYFYLLLSGLS 549

Query: 545 VLAFVAFLF 554
           +LAFV FLF
Sbjct: 550 LLAFVGFLF 557

BLAST of Lag0039768 vs. NCBI nr
Match: XP_038891005.1 (protein NRT1/ PTR FAMILY 5.10-like [Benincasa hispida])

HSP 1 Score: 944.5 bits (2440), Expect = 4.2e-271
Identity = 479/561 (85.38%), Postives = 519/561 (92.51%), Query Frame = 0

Query: 1   MDRRTPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLT 60
           M+  TPLL +TV+GA+DF+GQPVLR SSGGW+SASFMIGVEVAERFAYYGIGSNLITYLT
Sbjct: 1   MEPGTPLLRQTVEGAVDFKGQPVLRFSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60

Query: 61  GPLGQSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSA 120
           GPLGQSVATAAE++NIWSG+SMLLTLLGAFLAD+FFGRYRTILFSSAIYVLGL+LLSFSA
Sbjct: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120

Query: 121 LLPTTTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQN 180
           +LPTTTS                +FQL+LFFVSLYLIGIGQGGHKPCVQAFGADQFDA +
Sbjct: 121 MLPTTTS------------PQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALD 180

Query: 181 PQEAKSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGT 240
           PQEAKSKSSFFNWWFFGVCAG+FVAIL+VTYTEENLSWSLGFGIPCI+M+IAS +F+ GT
Sbjct: 181 PQEAKSKSSFFNWWFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLLGT 240

Query: 241 KTYRYSVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKA 300
            TYRY++K Y K+PF+RIGRVFVSA+RNWRASSTV FDEE  GRDLSQQNAGQFRFLNKA
Sbjct: 241 NTYRYTIKTYDKTPFLRIGRVFVSAIRNWRASSTVIFDEEGNGRDLSQQNAGQFRFLNKA 300

Query: 301 CIVPNHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSI 360
           CIVP  SN C M+CSVSEVEEAKAVLRIFP+W+TVLVF IVF QDSTFFTKQGATMDRSI
Sbjct: 301 CIVPKDSNKCGMMCSVSEVEEAKAVLRIFPIWITVLVFAIVFSQDSTFFTKQGATMDRSI 360

Query: 361 LSGFIIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAIS 420
           LSGFIIPAAALD+FVPLSIVIF+TIYDL+FVP+ARAFTGIQSGITTLQRIGTGL+VSAIS
Sbjct: 361 LSGFIIPAAALDAFVPLSIVIFVTIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAIS 420

Query: 421 MLVAAMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQ 480
           MLVA MVERKRLRIA++HGLVD+PDITIP+SFWWLVPQYTLFGLAE FTLVGLQEFFYDQ
Sbjct: 421 MLVATMVERKRLRIADKHGLVDKPDITIPISFWWLVPQYTLFGLAEVFTLVGLQEFFYDQ 480

Query: 481 CPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLL 540
            PTDLKSMGLAFYTSVLGMGSILSSLLVSVIDE TG + QNSWFSNNLNKAHLDYFYLLL
Sbjct: 481 VPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEVTGGSEQNSWFSNNLNKAHLDYFYLLL 540

Query: 541 SGLSVLAFVAFLFVSNSHVHS 562
           SGLSV+AFVAFLFVS SHVHS
Sbjct: 541 SGLSVVAFVAFLFVSKSHVHS 549

BLAST of Lag0039768 vs. NCBI nr
Match: XP_008459680.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo])

HSP 1 Score: 921.0 bits (2379), Expect = 4.9e-264
Identity = 471/561 (83.96%), Postives = 511/561 (91.09%), Query Frame = 0

Query: 1   MDRRTPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLT 60
           M++ TPLL++TV+ A+DF+GQP LR  SGGW+SASFMIGVEVAERFAYYGIGSNLITYLT
Sbjct: 1   MEQPTPLLSQTVESAVDFKGQPALRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60

Query: 61  GPLGQSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSA 120
           GPLGQSVATAAE++NIWSG+SMLLTLLGAFLAD+FFGRYRTILFSSAIYVLGL+LLSFSA
Sbjct: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120

Query: 121 LLPTTTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQN 180
           +LPTT+S +              +FQL+ FFVSLYLIGIGQGGHKPCVQAFGADQFDA +
Sbjct: 121 MLPTTSSQN-------------SQFQLIFFFVSLYLIGIGQGGHKPCVQAFGADQFDALH 180

Query: 181 PQEAKSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGT 240
           PQEAKSKSSFFNWWFFGVCAG+FVAIL+VTYTEENLSWSLGFGIPCI+MVIAS +F+ GT
Sbjct: 181 PQEAKSKSSFFNWWFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMVIASFLFLFGT 240

Query: 241 KTYRYSVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKA 300
            TYRYS+K Y K+PF+RIGRVFVSA+RN RASSTV FDEE  G DLS+QN GQF FLNKA
Sbjct: 241 NTYRYSIKMYAKTPFLRIGRVFVSAIRNCRASSTVTFDEEGDGPDLSEQNTGQFSFLNKA 300

Query: 301 CIVPNHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSI 360
           CIVP  SN   ++CS SEVEEAKAVLRIFPVW+TVLVF IVF QDSTFFTKQGAT+DRSI
Sbjct: 301 CIVPKDSNKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSI 360

Query: 361 LSGFIIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAIS 420
           LSGFIIPAAALDSFVPLSIVIFITIYDL+FVP+ARAFTGIQSGITTLQRIGTGL+VSAIS
Sbjct: 361 LSGFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAIS 420

Query: 421 MLVAAMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQ 480
           MLVA MVERKRLRIA+EHGLVDRPDITIPMSFWWLVPQYTLFGLAE FTLVGLQEFFYDQ
Sbjct: 421 MLVATMVERKRLRIADEHGLVDRPDITIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQ 480

Query: 481 CPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLL 540
            PTDLKSMGLAFYTSVLGMGSILSSLLVS+IDE TG + QNSWFSNNLNKAHLDYFYLLL
Sbjct: 481 VPTDLKSMGLAFYTSVLGMGSILSSLLVSIIDEVTGGSEQNSWFSNNLNKAHLDYFYLLL 540

Query: 541 SGLSVLAFVAFLFVSNSHVHS 562
           S LSV+AFVAFLFVS SHVHS
Sbjct: 541 SALSVVAFVAFLFVSKSHVHS 548

BLAST of Lag0039768 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 1.8e-184
Identity = 321/555 (57.84%), Postives = 424/555 (76.40%), Query Frame = 0

Query: 5   TPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64
           TPLLA TV    D++ +P ++SSSGGW+SA F+IGVEVAERFAYYGI SNLITYLTGPLG
Sbjct: 12  TPLLAVTV----DYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLG 71

Query: 65  QSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLPT 124
           QS A AA ++N WSG + LL LLGAF+AD+F GR+RTIL +SA+Y++GL +L+ SA++P 
Sbjct: 72  QSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIP- 131

Query: 125 TTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQEA 184
              S C  +  +SSCS  P+FQ++ FF +LYL+ + QGGHKPCVQAFGADQFD + P+E 
Sbjct: 132 ---SDCKVSNLLSSCS--PRFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEEC 191

Query: 185 KSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTYR 244
           K+KSSFFNWW+FG+C G+   + V+ Y ++NLSW+LGFGIPCI MV+A VV + GT TYR
Sbjct: 192 KAKSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYR 251

Query: 245 YSVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKACIVP 304
           +S+++  +SPFVRIG V+V+AV+NW  S+      EE    +S  ++ QF FLNKA +  
Sbjct: 252 FSIRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAK 311

Query: 305 NHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSILSGF 364
           N S      CS+ E+EEAK+VLR+ P+W+T LV+ +VF Q  TFFTKQGATM+RSI  G+
Sbjct: 312 NGS------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGY 371

Query: 365 IIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISMLVA 424
            I  A L SF+ LSIVIFI IYD V +P+AR+FT    GIT LQRIGTG+ +S ++M+VA
Sbjct: 372 KISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVA 431

Query: 425 AMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQCPTD 484
           A+VE KRL+ A ++GLVD PD T+PMS WWLVPQY LFG+ + F +VGLQEFFYDQ P +
Sbjct: 432 ALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNE 491

Query: 485 LKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLSGLS 544
           L+S+GLA Y S+ G+G+ LSS ++S+I++AT  +GQ SWF+NNLN+AHLDYFY LL+ LS
Sbjct: 492 LRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLS 550

Query: 545 VLAFVAFLFVSNSHV 560
            +   ++L+V+ S+V
Sbjct: 552 FIGLASYLYVAKSYV 550

BLAST of Lag0039768 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 600.1 bits (1546), Expect = 2.5e-170
Identity = 310/556 (55.76%), Postives = 404/556 (72.66%), Query Frame = 0

Query: 7   LLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPLGQS 66
           L  E V  A+D +G    RS++G W++A F+IGVEVAERFAYYGIGSNLI+YLTGPLG+S
Sbjct: 9   LQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGES 68

Query: 67  VATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLPTTT 126
            A AA ++N WSG++ LL +LGAF+AD F GRYRTI+ SS IYVLGLA L+ SA L    
Sbjct: 69  TAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFL---- 128

Query: 127 SSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQEAKS 186
                NT E++  SST  F  +LFF SLYL+ IGQ GHKPCVQAFGADQFD ++ QE   
Sbjct: 129 ---IPNTTEVT--SSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSD 188

Query: 187 KSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTYRYS 246
           +SSFFNWW+  + AG   AILVV Y +E  SW+ GFGIPC+ MVI+ V+FVSG + YRYS
Sbjct: 189 RSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYS 248

Query: 247 VKKYHK--SPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKACIVP 306
            +++ +  +PF RIGRVF  A++N R SS+     E       ++ +    F NKA +VP
Sbjct: 249 KRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVELEANTSPEKQS----FFNKALLVP 308

Query: 307 NHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSILSGF 366
           N S+        S+VE+A A++R+ PVW T L + I + Q  TFFTKQG TMDR+IL G 
Sbjct: 309 NDSSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGV 368

Query: 367 IIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISMLVA 426
            IP A+L  F+ +SIV+F+ IYD VFVP+AR  T    GITTL+RIGTG+++S I+M++A
Sbjct: 369 KIPPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIA 428

Query: 427 AMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQCPTD 486
           A+VE KRL  A+EHGL+D+P+ T+PMS WWL+PQY L GLA+ +TLVG+QEFFY Q PT+
Sbjct: 429 ALVEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTE 488

Query: 487 LKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLSGLS 546
           L+S+GLA Y S LG+GS+LSSLL+S+ID ATG +  NSWF++NLN+AHLDYFY LL+ +S
Sbjct: 489 LRSIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVS 548

Query: 547 VLAFVAFLFVSNSHVH 561
            + F  FLF+S S+++
Sbjct: 549 AVGFFTFLFISKSYIY 551

BLAST of Lag0039768 vs. ExPASy Swiss-Prot
Match: Q0WSZ6 (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 592.0 bits (1525), Expect = 6.9e-168
Identity = 306/559 (54.74%), Postives = 403/559 (72.09%), Query Frame = 0

Query: 4   RTPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPL 63
           +T L  E V  A+D +G    RS +G W++A F+IGVEVAERFA YGIGSNLI+YLTGPL
Sbjct: 6   KTSLQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPL 65

Query: 64  GQSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLP 123
           GQS A AA ++N WSG+S +L LLGAF+AD F GRY TI+ +S IYVLGLA L+ SA L 
Sbjct: 66  GQSTAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFL- 125

Query: 124 TTTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQE 183
                   N  E++S  S+  F   LFF SLYL+ IGQ GHKPCVQAFGADQFD +NPQE
Sbjct: 126 ------IPNNTEVTSSPSS--FLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQE 185

Query: 184 AKSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTY 243
              +SSFFNWW+  +CAG  +AILVV Y +EN+SW+LGFGIPC+ MVI+ V+FV G K+Y
Sbjct: 186 NSDRSSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSY 245

Query: 244 RYSVKKYHK--SPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKAC 303
           R+S  +  +  +PF RIGRVF  A +N R +S+     E    + SQ++  +  FLNKA 
Sbjct: 246 RFSKTRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKAL 305

Query: 304 IVPNHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSIL 363
           +VPN S+   + C   +VE+A A++R+ PVW+T L + I F Q  TFFTKQG TM+R+I 
Sbjct: 306 LVPNDSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIF 365

Query: 364 SGFIIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISM 423
            G  IP A+L   + +SIV+F+ IYD V VP+ R+ T    GITTL+RIGTG++++ ++M
Sbjct: 366 PGVEIPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTM 425

Query: 424 LVAAMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQC 483
           +VAA+VE KRL  A+E+GL+D+P  T+PMS WWL PQY L GLA+  TLVG+QEFFY Q 
Sbjct: 426 VVAALVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQV 485

Query: 484 PTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLS 543
           PT+L+S+GLA Y S +G+GS+LSSLL+ +ID ATG +  NSWF++NLN+AHLDYFY LL+
Sbjct: 486 PTELRSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLA 545

Query: 544 GLSVLAFVAFLFVSNSHVH 561
            +S + F  FLF+S S+++
Sbjct: 546 VVSAVGFFTFLFISKSYIY 555

BLAST of Lag0039768 vs. ExPASy Swiss-Prot
Match: Q9C7U1 (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 1.7e-158
Identity = 292/559 (52.24%), Postives = 396/559 (70.84%), Query Frame = 0

Query: 10  ETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPLGQSVAT 69
           E V+ ++DF+G P +RSSSG WKS+ F +  EVAE+FAY+GI SNLITY T  LG+S A 
Sbjct: 17  ENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTAV 76

Query: 70  AAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLPTTTSSH 129
           AA ++N+W G +  L L+   +AD+F GR+RTIL +S+ Y++GL LL+FSA +P    S 
Sbjct: 77  AASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIP----SL 136

Query: 130 CHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQEAKSKSS 189
           C++     SC S  K  +++FF +LYLI +G+GG K C++AFGADQFD Q+P E+K+KSS
Sbjct: 137 CNDQETRESCVSQVK--VIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSS 196

Query: 190 FFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTYRYSV-- 249
           +FNW +F +  G     LV  Y +ENLSW+LG+ IPC+ M++A  +F+ G KTYR+S   
Sbjct: 197 YFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGG 256

Query: 250 ----KKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKACIV 309
                K H +PFVRIGRVFV+A RN R + +      +    L  ++  +FRFL++A I 
Sbjct: 257 EGRQGKKHDNPFVRIGRVFVAAARNRRQTPS------DTCLLLPNESTKKFRFLDRAVI- 316

Query: 310 PNHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSILSG 369
                     C   EVEEAKAVL + P+W+  LVFGIVF Q  TFFTKQG+TMDRSI S 
Sbjct: 317 ---------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISST 376

Query: 370 FIIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISMLV 429
             +PAA L  F+ L+I++FI IYD +FVP+AR+ T   +GITTLQRI TG+ +S ISM++
Sbjct: 377 LQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVI 436

Query: 430 AAMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQCPT 489
           AA+VE KRL+ A +HGLVD P  T+PMS  WL+PQY LFG+++ FT+VGLQEFFY + P 
Sbjct: 437 AALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPP 496

Query: 490 DLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLSGL 549
            L+SMGLA Y S++G+G+ LSS +VSVI+EAT  +GQ SWFSNNLN+AHLDYFY LL+ L
Sbjct: 497 QLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACL 553

Query: 550 SVLAFVAFLFVSNSHVHSS 563
           S LAF+  ++ + S++++S
Sbjct: 557 SSLAFIFTVYFAKSYLYNS 553

BLAST of Lag0039768 vs. ExPASy Swiss-Prot
Match: Q9SK96 (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 7.1e-157
Identity = 290/564 (51.42%), Postives = 398/564 (70.57%), Query Frame = 0

Query: 2   DRRTPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTG 61
           +    L+ ++V  ++D +G P  +SS+GGW+SA ++IGVEV ERFAY+GIGSNLITYLTG
Sbjct: 5   EEEAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTG 64

Query: 62  PLGQSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSA- 121
           PLGQS ATAA ++N WSG + +L +LGAF+AD + GRYRTI+ +S IY+LGL LL+ S+ 
Sbjct: 65  PLGQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSI 124

Query: 122 -LLPTTTSSHCHNTYEISSCSSTPKFQL-MLFFVSLYLIGIGQGGHKPCVQAFGADQFDA 181
            +L   +    HN     + S+ P F + +LFF SLYL+ IGQGGHKPCVQAFGADQFD 
Sbjct: 125 LILMGLSEQRQHN----RNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDV 184

Query: 182 QNPQEAKSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVS 241
            +P+E  S+ SFFNWWF  + AG  ++I+VV Y ++N++W+LGFGIPC+ MV+A  +F+ 
Sbjct: 185 GDPKERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLF 244

Query: 242 GTKTYRY--SVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRF 301
           G KTYRY    ++   + F RIGRVF+ A +N +   T +   E G     ++  GQ  F
Sbjct: 245 GRKTYRYPRGDREGKNNAFARIGRVFLVAFKNRKLKLTHSGQLEVGS---YKKCKGQLEF 304

Query: 302 LNKACIVPNHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATM 361
           L KA ++P         CS  +VE+A A++R+ P+W+T +V  I + Q +TFFTKQG T+
Sbjct: 305 LAKA-LLPGEGGV--EPCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTV 364

Query: 362 DRSILSGFIIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIV 421
           DR IL GF IP A+  + + LSI I +  Y+ VF+P+AR  T   SGIT LQRIG G+++
Sbjct: 365 DRKILPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVL 424

Query: 422 SAISMLVAAMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEF 481
           S+++M+VAA+VE KRL  A+EHGLVDRPD TIPMS WW VPQY L G+ + F+LVG QEF
Sbjct: 425 SSLNMVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEF 484

Query: 482 FYDQCPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYF 541
           FYDQ PT+L+S+GLA   S +G+ S LS  L++VI+ ATG NG +SWF+ NLN+AH+DYF
Sbjct: 485 FYDQVPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYF 544

Query: 542 YLLLSGLSVLAFVAFLFVSNSHVH 561
           Y LL+  + + F+AFL +S  +V+
Sbjct: 545 YWLLAAFTAIGFLAFLLLSRLYVY 558

BLAST of Lag0039768 vs. ExPASy TrEMBL
Match: A0A6J1GRM0 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111456880 PE=3 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.5e-279
Identity = 497/557 (89.23%), Postives = 525/557 (94.25%), Query Frame = 0

Query: 5   TPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64
           +PLL +TVDGA+DFQG PVLRSSSGGW+SASFMIGVEVAERFAYYGIG+NLITYLTGPLG
Sbjct: 10  SPLLNDTVDGAVDFQGHPVLRSSSGGWRSASFMIGVEVAERFAYYGIGANLITYLTGPLG 69

Query: 65  QSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLPT 124
           QSVATAAES+NIWSG+SML TLLGAF+AD+FFGRYRTILFSSAIYVLGLALLSFSALLPT
Sbjct: 70  QSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLALLSFSALLPT 129

Query: 125 TTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQEA 184
           TTS  C N+ EISSC STP+FQLMLFFVSLYLIG+GQGGHKPCVQAFGADQFDAQ+PQEA
Sbjct: 130 TTSLLCQNSNEISSC-STPQFQLMLFFVSLYLIGVGQGGHKPCVQAFGADQFDAQHPQEA 189

Query: 185 KSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTYR 244
           KSKSSFFNWWFFGVCAG+FVAILVVTYTEENLSWSLGFGIP I+MVIASVVFV G  TYR
Sbjct: 190 KSKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVFVFGKNTYR 249

Query: 245 YSVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKACIVP 304
           YSVKKY KSPFVRIGRVFVSA RNWRASST  F +EE   DLSQQ AGQFRFLNKACIVP
Sbjct: 250 YSVKKYDKSPFVRIGRVFVSAARNWRASSTFIFGQEESAIDLSQQKAGQFRFLNKACIVP 309

Query: 305 NHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSILSGF 364
           NHSN C MVCS+SEVEEAKAVLRIFPVWMTVLVFGIVF QDSTFFTKQGATMDRSILSGF
Sbjct: 310 NHSNLCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRSILSGF 369

Query: 365 IIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISMLVA 424
           IIPAAA+DSFVPLSIVIFITIYD VFV +ARAFTG+QSGITTLQRIGTGL++SAISMLVA
Sbjct: 370 IIPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGVQSGITTLQRIGTGLVISAISMLVA 429

Query: 425 AMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQCPTD 484
           +MVERKRLRIAEE GLVDRPDITIPMSFWWLVPQYTL+GLAE FTLVGLQEFFYDQCP+D
Sbjct: 430 SMVERKRLRIAEEQGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQEFFYDQCPSD 489

Query: 485 LKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLSGLS 544
           LKSMGLAFYTSVLGMGSILSSLLVSVIDEATG NGQNSWFS+NLNKAHLDYFYLLLSGLS
Sbjct: 490 LKSMGLAFYTSVLGMGSILSSLLVSVIDEATGANGQNSWFSDNLNKAHLDYFYLLLSGLS 549

Query: 545 VLAFVAFLFVSNSHVHS 562
           +LAFV FLFVS  HV+S
Sbjct: 550 LLAFVGFLFVSTWHVYS 565

BLAST of Lag0039768 vs. ExPASy TrEMBL
Match: A0A6J1K2A2 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111490020 PE=3 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 7.9e-276
Identity = 490/549 (89.25%), Postives = 518/549 (94.35%), Query Frame = 0

Query: 5   TPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64
           +PLL +TVDG +DFQG PVLRSS+GGW+SASFMIGVEVAERFAYYGIG+NLITYLTGPLG
Sbjct: 10  SPLLNDTVDGTVDFQGHPVLRSSAGGWRSASFMIGVEVAERFAYYGIGANLITYLTGPLG 69

Query: 65  QSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLPT 124
           QSVATAAES+NIWSG+SML TLLGAF+AD+FFGRYRTILFSSAIYVLGLALLSFSALLPT
Sbjct: 70  QSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLALLSFSALLPT 129

Query: 125 TTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQEA 184
           TTS  C N+ EISSC STP+FQL+LFFVSLYLIG+GQGGHKPCVQAFGADQFDAQ+PQEA
Sbjct: 130 TTSLLCQNSNEISSC-STPQFQLVLFFVSLYLIGVGQGGHKPCVQAFGADQFDAQHPQEA 189

Query: 185 KSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTYR 244
           KSKSSFFNWWFFGVCAG+FVAILVVTYTEENLSWSLGFGIP I+MVIASVVFV G  TYR
Sbjct: 190 KSKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVFVFGKYTYR 249

Query: 245 YSVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKACIVP 304
           YSVKKY KSPFVRIGRVFVSA RNWRASST  FD+EE   DLSQQ AGQFRFLNKACIVP
Sbjct: 250 YSVKKYDKSPFVRIGRVFVSAARNWRASSTFIFDQEESATDLSQQKAGQFRFLNKACIVP 309

Query: 305 NHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSILSGF 364
           NHSN C MVCS+SEVEEAKAVLRIFPVWMTVLVFGIVF QDSTFFTKQGATMDRSILSGF
Sbjct: 310 NHSNQCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRSILSGF 369

Query: 365 IIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISMLVA 424
           IIPAAA+DSFVPLSIVIFITIYD VFV +ARAFTGIQSGITTLQRIGTGL++SAISMLVA
Sbjct: 370 IIPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGIQSGITTLQRIGTGLVISAISMLVA 429

Query: 425 AMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQCPTD 484
           +MVERKRLRIAEE GLVDRPDITIPMSFWWLVPQYTL+GLAE FTLVGLQEFFYDQCP+D
Sbjct: 430 SMVERKRLRIAEETGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQEFFYDQCPSD 489

Query: 485 LKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLSGLS 544
           LKSMGLAFYTSVLG GSILSSLLVSVIDEATG NGQNSWFS+NLNKAHLDYFYLLLSGLS
Sbjct: 490 LKSMGLAFYTSVLGTGSILSSLLVSVIDEATGANGQNSWFSDNLNKAHLDYFYLLLSGLS 549

Query: 545 VLAFVAFLF 554
           +LAFV FLF
Sbjct: 550 LLAFVGFLF 557

BLAST of Lag0039768 vs. ExPASy TrEMBL
Match: A0A1S3CAV1 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103498725 PE=3 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 2.4e-264
Identity = 471/561 (83.96%), Postives = 511/561 (91.09%), Query Frame = 0

Query: 1   MDRRTPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLT 60
           M++ TPLL++TV+ A+DF+GQP LR  SGGW+SASFMIGVEVAERFAYYGIGSNLITYLT
Sbjct: 1   MEQPTPLLSQTVESAVDFKGQPALRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60

Query: 61  GPLGQSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSA 120
           GPLGQSVATAAE++NIWSG+SMLLTLLGAFLAD+FFGRYRTILFSSAIYVLGL+LLSFSA
Sbjct: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120

Query: 121 LLPTTTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQN 180
           +LPTT+S +              +FQL+ FFVSLYLIGIGQGGHKPCVQAFGADQFDA +
Sbjct: 121 MLPTTSSQN-------------SQFQLIFFFVSLYLIGIGQGGHKPCVQAFGADQFDALH 180

Query: 181 PQEAKSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGT 240
           PQEAKSKSSFFNWWFFGVCAG+FVAIL+VTYTEENLSWSLGFGIPCI+MVIAS +F+ GT
Sbjct: 181 PQEAKSKSSFFNWWFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMVIASFLFLFGT 240

Query: 241 KTYRYSVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKA 300
            TYRYS+K Y K+PF+RIGRVFVSA+RN RASSTV FDEE  G DLS+QN GQF FLNKA
Sbjct: 241 NTYRYSIKMYAKTPFLRIGRVFVSAIRNCRASSTVTFDEEGDGPDLSEQNTGQFSFLNKA 300

Query: 301 CIVPNHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSI 360
           CIVP  SN   ++CS SEVEEAKAVLRIFPVW+TVLVF IVF QDSTFFTKQGAT+DRSI
Sbjct: 301 CIVPKDSNKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSI 360

Query: 361 LSGFIIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAIS 420
           LSGFIIPAAALDSFVPLSIVIFITIYDL+FVP+ARAFTGIQSGITTLQRIGTGL+VSAIS
Sbjct: 361 LSGFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAIS 420

Query: 421 MLVAAMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQ 480
           MLVA MVERKRLRIA+EHGLVDRPDITIPMSFWWLVPQYTLFGLAE FTLVGLQEFFYDQ
Sbjct: 421 MLVATMVERKRLRIADEHGLVDRPDITIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQ 480

Query: 481 CPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLL 540
            PTDLKSMGLAFYTSVLGMGSILSSLLVS+IDE TG + QNSWFSNNLNKAHLDYFYLLL
Sbjct: 481 VPTDLKSMGLAFYTSVLGMGSILSSLLVSIIDEVTGGSEQNSWFSNNLNKAHLDYFYLLL 540

Query: 541 SGLSVLAFVAFLFVSNSHVHS 562
           S LSV+AFVAFLFVS SHVHS
Sbjct: 541 SALSVVAFVAFLFVSKSHVHS 548

BLAST of Lag0039768 vs. ExPASy TrEMBL
Match: A0A6J1DNR2 (protein NRT1/ PTR FAMILY 5.10-like OS=Momordica charantia OX=3673 GN=LOC111022354 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.5e-263
Identity = 462/561 (82.35%), Postives = 511/561 (91.09%), Query Frame = 0

Query: 2   DRRTPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTG 61
           +RRTPLL++TVDGA+DF+GQPVLRSSSGGW+SASF+IGVEVAERFAYYGIGSNLITYLTG
Sbjct: 8   NRRTPLLSDTVDGAVDFKGQPVLRSSSGGWRSASFIIGVEVAERFAYYGIGSNLITYLTG 67

Query: 62  PLGQSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSAL 121
           PLG SVATAAES+N+WSG SMLLTLLGAF+AD+FFG YRTIL SSAIYVLGL LLSFSAL
Sbjct: 68  PLGLSVATAAESLNVWSGTSMLLTLLGAFVADSFFGPYRTILLSSAIYVLGLGLLSFSAL 127

Query: 122 LPTTTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNP 181
           LPTT SS  HN           K Q++LFFVSLYLIGIGQGGHKPCVQAFGADQFD ++P
Sbjct: 128 LPTTNSSVSHN-----------KLQIILFFVSLYLIGIGQGGHKPCVQAFGADQFDGRHP 187

Query: 182 QEAKSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTK 241
           +E KSKSSFFNWWFFG+CAG+FVA+LVVTYTEEN+SWSLGFGIPC+ MVIA VVF+ GT 
Sbjct: 188 EEVKSKSSFFNWWFFGLCAGTFVAMLVVTYTEENVSWSLGFGIPCLAMVIALVVFLFGTN 247

Query: 242 TYRYSVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKAC 301
           TYRYSVK+Y KSPFVRIGRVFVSA+RNWRAS TV FDEE+GGR LS QNAGQFRFLNKAC
Sbjct: 248 TYRYSVKEYGKSPFVRIGRVFVSAIRNWRASPTVFFDEEDGGRVLSHQNAGQFRFLNKAC 307

Query: 302 IVPNHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSIL 361
            VPNHS+ C M CS+SEVEEAKAVLRI P+W+T+LVF IVF QD+TFFTKQGATM+RSI 
Sbjct: 308 AVPNHSSQCGMACSISEVEEAKAVLRILPIWITILVFAIVFAQDTTFFTKQGATMNRSIF 367

Query: 362 SGFIIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISM 421
           SGFIIPAAAL+S VPLSIVIFI++YDLVFVPVARAFTGIQSGIT LQRIGTGL++SAISM
Sbjct: 368 SGFIIPAAALESLVPLSIVIFISVYDLVFVPVARAFTGIQSGITPLQRIGTGLVISAISM 427

Query: 422 LVAAMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQC 481
           +VA MVERKRLR AEEHG+VD PDITIPMSFWWLVPQY+L+GLA+ FT+VGLQEFFY+QC
Sbjct: 428 VVATMVERKRLRSAEEHGVVDSPDITIPMSFWWLVPQYSLYGLADVFTVVGLQEFFYNQC 487

Query: 482 PTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLS 541
           P DLKSMGLA YTSVLGMGSILSS+LVSVIDEA+G NG NSWFS+NLNKAHLDYFYLLLS
Sbjct: 488 PADLKSMGLALYTSVLGMGSILSSVLVSVIDEASGGNGHNSWFSDNLNKAHLDYFYLLLS 547

Query: 542 GLSVLAFVAFLFVSNSHVHSS 563
           GLSV+AFVAFLFVS SHV+SS
Sbjct: 548 GLSVVAFVAFLFVSKSHVYSS 557

BLAST of Lag0039768 vs. ExPASy TrEMBL
Match: A0A5A7T8K1 (Proton-dependent oligopeptide transporter family OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00050 PE=3 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 2.0e-247
Identity = 449/562 (79.89%), Postives = 488/562 (86.83%), Query Frame = 0

Query: 1   MDRRTPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLT 60
           M++ TPLL++TV+ A+DF+GQP LR  SGGW+SASFMIGVEVAERFAYYGIGSNLITYLT
Sbjct: 102 MEQPTPLLSQTVESAVDFKGQPALRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 161

Query: 61  GPLGQSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSA 120
           GPLGQSVATAAE++NIWSG+SMLLTLLGAFLAD+FFGRYRTILFSSAIYVLGL+LLSFSA
Sbjct: 162 GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 221

Query: 121 LLPTTTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQN 180
           +LPTT+S +              +FQL+ FFVSLYLIGIGQGGHKPCVQAFGADQFDA +
Sbjct: 222 MLPTTSSQN-------------SQFQLIFFFVSLYLIGIGQGGHKPCVQAFGADQFDALH 281

Query: 181 PQEAKSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGT 240
           PQEAKSKSSFFNWWFFGVCAG+FVAIL+VTYTEENLSWSLGFGIPCI+MVIAS +F+ GT
Sbjct: 282 PQEAKSKSSFFNWWFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMVIASFLFLFGT 341

Query: 241 KTYRYSVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKA 300
            TYRYS+K Y K+PF+RIGRVFVSA+RN RASSTV FDEE  G DLS+QN GQF FLNKA
Sbjct: 342 NTYRYSIKMYAKTPFLRIGRVFVSAIRNCRASSTVTFDEEGDGPDLSEQNTGQFSFLNKA 401

Query: 301 CIVPNHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSI 360
           CIVP  SN   ++CS SE                          DSTFFTKQGAT+DRSI
Sbjct: 402 CIVPKDSNKHGVMCSASE--------------------------DSTFFTKQGATIDRSI 461

Query: 361 LSGFIIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAIS 420
           LSGFIIPAAALDSFVPLSIVIFITIYDL+FVP+ARAFTGIQSGITTLQRIGTGL+VSAIS
Sbjct: 462 LSGFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAIS 521

Query: 421 MLVAAMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQ 480
           MLVA MVERKRLRIA+EHGLVDRPDITIPMSFWWLVPQYTLFGLAE FTLVGLQEFFYDQ
Sbjct: 522 MLVATMVERKRLRIADEHGLVDRPDITIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQ 581

Query: 481 CPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLL 540
            PTDLKSMGLAFYTSVLGMGSILSSLLVS+IDE TG + QNSWFSNNLNKAHLDYFYLLL
Sbjct: 582 VPTDLKSMGLAFYTSVLGMGSILSSLLVSIIDEVTGGSEQNSWFSNNLNKAHLDYFYLLL 624

Query: 541 SGLSVLAFVAFLFVSNSHVHSS 563
           S LSV+AFVAFLFVS SHVHSS
Sbjct: 642 SALSVVAFVAFLFVSKSHVHSS 624

BLAST of Lag0039768 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 647.1 bits (1668), Expect = 1.3e-185
Identity = 321/555 (57.84%), Postives = 424/555 (76.40%), Query Frame = 0

Query: 5   TPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64
           TPLLA TV    D++ +P ++SSSGGW+SA F+IGVEVAERFAYYGI SNLITYLTGPLG
Sbjct: 12  TPLLAVTV----DYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLG 71

Query: 65  QSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLPT 124
           QS A AA ++N WSG + LL LLGAF+AD+F GR+RTIL +SA+Y++GL +L+ SA++P 
Sbjct: 72  QSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIP- 131

Query: 125 TTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQEA 184
              S C  +  +SSCS  P+FQ++ FF +LYL+ + QGGHKPCVQAFGADQFD + P+E 
Sbjct: 132 ---SDCKVSNLLSSCS--PRFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEEC 191

Query: 185 KSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTYR 244
           K+KSSFFNWW+FG+C G+   + V+ Y ++NLSW+LGFGIPCI MV+A VV + GT TYR
Sbjct: 192 KAKSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYR 251

Query: 245 YSVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKACIVP 304
           +S+++  +SPFVRIG V+V+AV+NW  S+      EE    +S  ++ QF FLNKA +  
Sbjct: 252 FSIRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAK 311

Query: 305 NHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSILSGF 364
           N S      CS+ E+EEAK+VLR+ P+W+T LV+ +VF Q  TFFTKQGATM+RSI  G+
Sbjct: 312 NGS------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGY 371

Query: 365 IIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISMLVA 424
            I  A L SF+ LSIVIFI IYD V +P+AR+FT    GIT LQRIGTG+ +S ++M+VA
Sbjct: 372 KISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVA 431

Query: 425 AMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQCPTD 484
           A+VE KRL+ A ++GLVD PD T+PMS WWLVPQY LFG+ + F +VGLQEFFYDQ P +
Sbjct: 432 ALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNE 491

Query: 485 LKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLSGLS 544
           L+S+GLA Y S+ G+G+ LSS ++S+I++AT  +GQ SWF+NNLN+AHLDYFY LL+ LS
Sbjct: 492 LRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLS 550

Query: 545 VLAFVAFLFVSNSHV 560
            +   ++L+V+ S+V
Sbjct: 552 FIGLASYLYVAKSYV 550

BLAST of Lag0039768 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 600.1 bits (1546), Expect = 1.8e-171
Identity = 310/556 (55.76%), Postives = 404/556 (72.66%), Query Frame = 0

Query: 7   LLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPLGQS 66
           L  E V  A+D +G    RS++G W++A F+IGVEVAERFAYYGIGSNLI+YLTGPLG+S
Sbjct: 9   LQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGES 68

Query: 67  VATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLPTTT 126
            A AA ++N WSG++ LL +LGAF+AD F GRYRTI+ SS IYVLGLA L+ SA L    
Sbjct: 69  TAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFL---- 128

Query: 127 SSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQEAKS 186
                NT E++  SST  F  +LFF SLYL+ IGQ GHKPCVQAFGADQFD ++ QE   
Sbjct: 129 ---IPNTTEVT--SSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSD 188

Query: 187 KSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTYRYS 246
           +SSFFNWW+  + AG   AILVV Y +E  SW+ GFGIPC+ MVI+ V+FVSG + YRYS
Sbjct: 189 RSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYS 248

Query: 247 VKKYHK--SPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKACIVP 306
            +++ +  +PF RIGRVF  A++N R SS+     E       ++ +    F NKA +VP
Sbjct: 249 KRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVELEANTSPEKQS----FFNKALLVP 308

Query: 307 NHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSILSGF 366
           N S+        S+VE+A A++R+ PVW T L + I + Q  TFFTKQG TMDR+IL G 
Sbjct: 309 NDSSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGV 368

Query: 367 IIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISMLVA 426
            IP A+L  F+ +SIV+F+ IYD VFVP+AR  T    GITTL+RIGTG+++S I+M++A
Sbjct: 369 KIPPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIA 428

Query: 427 AMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQCPTD 486
           A+VE KRL  A+EHGL+D+P+ T+PMS WWL+PQY L GLA+ +TLVG+QEFFY Q PT+
Sbjct: 429 ALVEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTE 488

Query: 487 LKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLSGLS 546
           L+S+GLA Y S LG+GS+LSSLL+S+ID ATG +  NSWF++NLN+AHLDYFY LL+ +S
Sbjct: 489 LRSIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVS 548

Query: 547 VLAFVAFLFVSNSHVH 561
            + F  FLF+S S+++
Sbjct: 549 AVGFFTFLFISKSYIY 551

BLAST of Lag0039768 vs. TAIR 10
Match: AT1G72125.1 (Major facilitator superfamily protein )

HSP 1 Score: 592.0 bits (1525), Expect = 4.9e-169
Identity = 306/559 (54.74%), Postives = 403/559 (72.09%), Query Frame = 0

Query: 4   RTPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPL 63
           +T L  E V  A+D +G    RS +G W++A F+IGVEVAERFA YGIGSNLI+YLTGPL
Sbjct: 6   KTSLQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPL 65

Query: 64  GQSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLP 123
           GQS A AA ++N WSG+S +L LLGAF+AD F GRY TI+ +S IYVLGLA L+ SA L 
Sbjct: 66  GQSTAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFL- 125

Query: 124 TTTSSHCHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQE 183
                   N  E++S  S+  F   LFF SLYL+ IGQ GHKPCVQAFGADQFD +NPQE
Sbjct: 126 ------IPNNTEVTSSPSS--FLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQE 185

Query: 184 AKSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTY 243
              +SSFFNWW+  +CAG  +AILVV Y +EN+SW+LGFGIPC+ MVI+ V+FV G K+Y
Sbjct: 186 NSDRSSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSY 245

Query: 244 RYSVKKYHK--SPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKAC 303
           R+S  +  +  +PF RIGRVF  A +N R +S+     E    + SQ++  +  FLNKA 
Sbjct: 246 RFSKTRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKAL 305

Query: 304 IVPNHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSIL 363
           +VPN S+   + C   +VE+A A++R+ PVW+T L + I F Q  TFFTKQG TM+R+I 
Sbjct: 306 LVPNDSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIF 365

Query: 364 SGFIIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISM 423
            G  IP A+L   + +SIV+F+ IYD V VP+ R+ T    GITTL+RIGTG++++ ++M
Sbjct: 366 PGVEIPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTM 425

Query: 424 LVAAMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQC 483
           +VAA+VE KRL  A+E+GL+D+P  T+PMS WWL PQY L GLA+  TLVG+QEFFY Q 
Sbjct: 426 VVAALVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQV 485

Query: 484 PTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLS 543
           PT+L+S+GLA Y S +G+GS+LSSLL+ +ID ATG +  NSWF++NLN+AHLDYFY LL+
Sbjct: 486 PTELRSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLA 545

Query: 544 GLSVLAFVAFLFVSNSHVH 561
            +S + F  FLF+S S+++
Sbjct: 546 VVSAVGFFTFLFISKSYIY 555

BLAST of Lag0039768 vs. TAIR 10
Match: AT1G72140.1 (Major facilitator superfamily protein )

HSP 1 Score: 560.8 bits (1444), Expect = 1.2e-159
Identity = 292/559 (52.24%), Postives = 396/559 (70.84%), Query Frame = 0

Query: 10  ETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTGPLGQSVAT 69
           E V+ ++DF+G P +RSSSG WKS+ F +  EVAE+FAY+GI SNLITY T  LG+S A 
Sbjct: 17  ENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTAV 76

Query: 70  AAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSALLPTTTSSH 129
           AA ++N+W G +  L L+   +AD+F GR+RTIL +S+ Y++GL LL+FSA +P    S 
Sbjct: 77  AASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIP----SL 136

Query: 130 CHNTYEISSCSSTPKFQLMLFFVSLYLIGIGQGGHKPCVQAFGADQFDAQNPQEAKSKSS 189
           C++     SC S  K  +++FF +LYLI +G+GG K C++AFGADQFD Q+P E+K+KSS
Sbjct: 137 CNDQETRESCVSQVK--VIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSS 196

Query: 190 FFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVSGTKTYRYSV-- 249
           +FNW +F +  G     LV  Y +ENLSW+LG+ IPC+ M++A  +F+ G KTYR+S   
Sbjct: 197 YFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGG 256

Query: 250 ----KKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRFLNKACIV 309
                K H +PFVRIGRVFV+A RN R + +      +    L  ++  +FRFL++A I 
Sbjct: 257 EGRQGKKHDNPFVRIGRVFVAAARNRRQTPS------DTCLLLPNESTKKFRFLDRAVI- 316

Query: 310 PNHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATMDRSILSG 369
                     C   EVEEAKAVL + P+W+  LVFGIVF Q  TFFTKQG+TMDRSI S 
Sbjct: 317 ---------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISST 376

Query: 370 FIIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIVSAISMLV 429
             +PAA L  F+ L+I++FI IYD +FVP+AR+ T   +GITTLQRI TG+ +S ISM++
Sbjct: 377 LQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVI 436

Query: 430 AAMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQCPT 489
           AA+VE KRL+ A +HGLVD P  T+PMS  WL+PQY LFG+++ FT+VGLQEFFY + P 
Sbjct: 437 AALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPP 496

Query: 490 DLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYFYLLLSGL 549
            L+SMGLA Y S++G+G+ LSS +VSVI+EAT  +GQ SWFSNNLN+AHLDYFY LL+ L
Sbjct: 497 QLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACL 553

Query: 550 SVLAFVAFLFVSNSHVHSS 563
           S LAF+  ++ + S++++S
Sbjct: 557 SSLAFIFTVYFAKSYLYNS 553

BLAST of Lag0039768 vs. TAIR 10
Match: AT1G22550.1 (Major facilitator superfamily protein )

HSP 1 Score: 555.4 bits (1430), Expect = 5.1e-158
Identity = 290/564 (51.42%), Postives = 398/564 (70.57%), Query Frame = 0

Query: 2   DRRTPLLAETVDGALDFQGQPVLRSSSGGWKSASFMIGVEVAERFAYYGIGSNLITYLTG 61
           +    L+ ++V  ++D +G P  +SS+GGW+SA ++IGVEV ERFAY+GIGSNLITYLTG
Sbjct: 5   EEEAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTG 64

Query: 62  PLGQSVATAAESINIWSGLSMLLTLLGAFLADTFFGRYRTILFSSAIYVLGLALLSFSA- 121
           PLGQS ATAA ++N WSG + +L +LGAF+AD + GRYRTI+ +S IY+LGL LL+ S+ 
Sbjct: 65  PLGQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSI 124

Query: 122 -LLPTTTSSHCHNTYEISSCSSTPKFQL-MLFFVSLYLIGIGQGGHKPCVQAFGADQFDA 181
            +L   +    HN     + S+ P F + +LFF SLYL+ IGQGGHKPCVQAFGADQFD 
Sbjct: 125 LILMGLSEQRQHN----RNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDV 184

Query: 182 QNPQEAKSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPCIVMVIASVVFVS 241
            +P+E  S+ SFFNWWF  + AG  ++I+VV Y ++N++W+LGFGIPC+ MV+A  +F+ 
Sbjct: 185 GDPKERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLF 244

Query: 242 GTKTYRY--SVKKYHKSPFVRIGRVFVSAVRNWRASSTVNFDEEEGGRDLSQQNAGQFRF 301
           G KTYRY    ++   + F RIGRVF+ A +N +   T +   E G     ++  GQ  F
Sbjct: 245 GRKTYRYPRGDREGKNNAFARIGRVFLVAFKNRKLKLTHSGQLEVGS---YKKCKGQLEF 304

Query: 302 LNKACIVPNHSNPCRMVCSVSEVEEAKAVLRIFPVWMTVLVFGIVFGQDSTFFTKQGATM 361
           L KA ++P         CS  +VE+A A++R+ P+W+T +V  I + Q +TFFTKQG T+
Sbjct: 305 LAKA-LLPGEGGV--EPCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTV 364

Query: 362 DRSILSGFIIPAAALDSFVPLSIVIFITIYDLVFVPVARAFTGIQSGITTLQRIGTGLIV 421
           DR IL GF IP A+  + + LSI I +  Y+ VF+P+AR  T   SGIT LQRIG G+++
Sbjct: 365 DRKILPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVL 424

Query: 422 SAISMLVAAMVERKRLRIAEEHGLVDRPDITIPMSFWWLVPQYTLFGLAEAFTLVGLQEF 481
           S+++M+VAA+VE KRL  A+EHGLVDRPD TIPMS WW VPQY L G+ + F+LVG QEF
Sbjct: 425 SSLNMVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEF 484

Query: 482 FYDQCPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGTNGQNSWFSNNLNKAHLDYF 541
           FYDQ PT+L+S+GLA   S +G+ S LS  L++VI+ ATG NG +SWF+ NLN+AH+DYF
Sbjct: 485 FYDQVPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYF 544

Query: 542 YLLLSGLSVLAFVAFLFVSNSHVH 561
           Y LL+  + + F+AFL +S  +V+
Sbjct: 545 YWLLAAFTAIGFLAFLLLSRLYVY 558

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022954701.13.2e-27989.23protein NRT1/ PTR FAMILY 5.10-like [Cucurbita moschata] >XP_022954702.1 protein ... [more]
XP_023541977.19.3e-27989.23protein NRT1/ PTR FAMILY 5.10-like [Cucurbita pepo subsp. pepo][more]
XP_022994214.11.6e-27589.25protein NRT1/ PTR FAMILY 5.10-like [Cucurbita maxima][more]
XP_038891005.14.2e-27185.38protein NRT1/ PTR FAMILY 5.10-like [Benincasa hispida][more]
XP_008459680.14.9e-26483.96PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q0WP011.8e-18457.84Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV... [more]
Q8VZE22.5e-17055.76Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV... [more]
Q0WSZ66.9e-16854.74Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV... [more]
Q9C7U11.7e-15852.24Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV... [more]
Q9SK967.1e-15751.42Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1GRM01.5e-27989.23protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111456880... [more]
A0A6J1K2A27.9e-27689.25protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111490020 P... [more]
A0A1S3CAV12.4e-26483.96protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103498725 PE=3 ... [more]
A0A6J1DNR21.5e-26382.35protein NRT1/ PTR FAMILY 5.10-like OS=Momordica charantia OX=3673 GN=LOC11102235... [more]
A0A5A7T8K12.0e-24779.89Proton-dependent oligopeptide transporter family OS=Cucumis melo var. makuwa OX=... [more]
Match NameE-valueIdentityDescription
AT1G22540.11.3e-18557.84Major facilitator superfamily protein [more]
AT1G72120.11.8e-17155.76Major facilitator superfamily protein [more]
AT1G72125.14.9e-16954.74Major facilitator superfamily protein [more]
AT1G72140.11.2e-15952.24Major facilitator superfamily protein [more]
AT1G22550.15.1e-15851.42Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 99..523
e-value: 1.7E-94
score: 316.9
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 17..560
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 7..562
e-value: 1.7E-192
score: 642.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 25..555
NoneNo IPR availablePANTHERPTHR11654:SF510FACILITATOR SUPERFAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATEDcoord: 17..560
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 88..112
IPR044739NRT1/PTR familyCDDcd17417MFS_NPF5coord: 31..559
e-value: 0.0
score: 632.766

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0039768.1Lag0039768.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0042938 dipeptide transport
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity