Lag0039701 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0039701 vs. NCBI nr
Match: KAG6592376.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 654.1 bits (1686), Expect = 1.1e-183
Identity = 352/541 (65.06%), Postives = 422/541 (78.00%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLQRDDLQLALETPTSIFKLPDSLTKDTPQSYSPSRLFLGPFHEL 60
           MAGTS  N V+E+ +LL+RD L++ALETPTSIF LPDS+TK+ PQ+Y+PSRL LGPFHEL
Sbjct: 1   MAGTSGMNWVVEVGELLRRDGLRMALETPTSIFMLPDSVTKENPQAYTPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLAVGSDALAWRI 120
             ELHKMEIFKLE AK  KE++QLP+LT +V+QLKE EV IRA Y ANLA+GSDALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPDLTLMVDQLKELEVPIRASYGANLAIGSDALASRI 120

Query: 121 LIDGLFLLRLLGIISPDDKEKEEKGKNSQLPYEVELPVFNEQQLYLNLDGNITT-TQNEI 180
           LIDGLFL+RLL I + +  ++    +     +++ELPVFNE++LYL L+ N+ T TQNEI
Sbjct: 121 LIDGLFLIRLLQIFADEKNDQGSSDRLHNRRHKLELPVFNEKELYLKLERNMATMTQNEI 180

Query: 181 VKDLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFSGS--DEEDDMPFEL 240
           +KDLLKLENQIPIQ+LKRVLP++L+ENL + FYKFCV VSPL L S       DD P   
Sbjct: 181 LKDLLKLENQIPIQVLKRVLPKDLIENLPILFYKFCVFVSPLSLPSADKFTTSDDNP--- 240

Query: 241 FPDYLRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGGERSSEILVELLNLMANIL 300
                  F  L EILD SQHL+HF YALL+YQRF AY      RS  + VELLNL+A ++
Sbjct: 241 -------FLNLSEILDQSQHLLHFFYALLYYQRFLAYMPSQDYRSGSLFVELLNLLAKVV 300

Query: 301 GIGLLKKLLEALGLIQTLIGFIGKVQKSSPFETDSSPRFPSASKLKKVGVKFDAGDGGGF 360
            +G++++L+EA+GLIQ+LIG +GK+  SSPFET++SP FPSA+KL+KVGVKF  GDGGGF
Sbjct: 301 QVGVVQQLVEAVGLIQSLIGMLGKIHHSSPFETETSPGFPSATKLRKVGVKFRGGDGGGF 360

Query: 361 DEASTTLKLPRITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILK 420
           D+ S TL LP +T+N F EVILRNLVAFEVEAKLNP  LS YVKLM+GLIAT KDV ILK
Sbjct: 361 DDESATLTLPIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVKLMNGLIATTKDVGILK 420

Query: 421 RAGIITGGRSGDEDDVVKMFKELGNVVNVSS-EGGESRANFKWYMNFEVDIIEKINVYYE 480
           RAGII GGRSGDEDDVVK+FK+LGNVV     +  E R+NF+ YMNFE  I E+I  YYE
Sbjct: 421 RAGIIVGGRSGDEDDVVKLFKDLGNVVMKGGFDSEEGRSNFESYMNFERRIGEEIMKYYE 480

Query: 481 NRWVVKAERFGKRYIVPMFQALLILVAVVLILIVAVRTVCGWFSCPKIMGHSIIHSGYYF 538
           +RW+VKAERF KRYI P+ Q L+ILV VVLILIVA+RTVCGWFSCPKIMGH II S Y  
Sbjct: 481 SRWMVKAERFVKRYIFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRIISSAYDM 531

BLAST of Lag0039701 vs. NCBI nr
Match: XP_022925375.1 (putative UPF0481 protein At3g02645 [Cucurbita moschata])

HSP 1 Score: 653.7 bits (1685), Expect = 1.4e-183
Identity = 351/541 (64.88%), Postives = 421/541 (77.82%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLQRDDLQLALETPTSIFKLPDSLTKDTPQSYSPSRLFLGPFHEL 60
           MAGTS TN V+E+ +LL+RD L++ALETPTSIFKLPDS+TK+ PQ+Y+PSRL LGPFHEL
Sbjct: 1   MAGTSGTNWVVEVGELLRRDGLRMALETPTSIFKLPDSVTKENPQAYTPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLAVGSDALAWRI 120
             ELHKMEIFKLE AK  KE++QLPELT +V+QLKE EV IRA Y   LA+GSDALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGTKLAIGSDALASRI 120

Query: 121 LIDGLFLLRLLGIISPDDKEKEEKGKNSQLPYEVELPVFNEQQLYLNLDGNITT-TQNEI 180
           LIDGLFL+RLL I + +  ++    +     +++ELPVF+EQ+LYL L+GN+ T T+NEI
Sbjct: 121 LIDGLFLIRLLQIFADEKNDQGSSDRLHNRRHKLELPVFDEQELYLKLEGNMATMTRNEI 180

Query: 181 VKDLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFSGS--DEEDDMPFEL 240
           +KDLLKLENQIPIQ+LKRVLP++L+ENL + FYKFCV VSPL L S       +D P   
Sbjct: 181 LKDLLKLENQIPIQVLKRVLPKDLIENLPILFYKFCVFVSPLSLPSADKFTTSNDNP--- 240

Query: 241 FPDYLRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGGERSSEILVELLNLMANIL 300
                  F  L EILD SQHL+HFLYALL+YQRF AY      RS  + VELLNL+A ++
Sbjct: 241 -------FLNLSEILDQSQHLLHFLYALLYYQRFLAYRPSQDYRSGSLFVELLNLLAKVV 300

Query: 301 GIGLLKKLLEALGLIQTLIGFIGKVQKSSPFETDSSPRFPSASKLKKVGVKFDAGDGGGF 360
            +G++++L+EA+GLIQ+LIG +GK+  SSPFET++SP  PSA KL+KVGVKF  GDGGGF
Sbjct: 301 QVGIVQQLVEAVGLIQSLIGMLGKIHHSSPFETETSPGLPSAMKLRKVGVKFHGGDGGGF 360

Query: 361 DEASTTLKLPRITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILK 420
           D+ S TL LP +T+N F EVILRNLVAFEVEAKLNP  LS YV LM+GLIAT KDV ILK
Sbjct: 361 DDESATLTLPIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVTLMNGLIATTKDVGILK 420

Query: 421 RAGIITGGRSGDEDDVVKMFKELGNVVNVSS-EGGESRANFKWYMNFEVDIIEKINVYYE 480
           RAGII GGRSGDEDDVVK+FK+LGNVV     +  E R+NF+ YMNFE  I E+I  YYE
Sbjct: 421 RAGIIVGGRSGDEDDVVKLFKDLGNVVMKGGFDSEEGRSNFESYMNFERRISEEIMKYYE 480

Query: 481 NRWVVKAERFGKRYIVPMFQALLILVAVVLILIVAVRTVCGWFSCPKIMGHSIIHSGYYF 538
           +RW+VKAERF KRYI P+ Q L+ILV VVLILIVA+RTVCGWFSCPKIMGH II S Y  
Sbjct: 481 SRWMVKAERFVKRYIFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRIISSAYDM 531

BLAST of Lag0039701 vs. NCBI nr
Match: XP_022973973.1 (UPF0481 protein At3g47200-like [Cucurbita maxima])

HSP 1 Score: 631.3 bits (1627), Expect = 7.5e-177
Identity = 349/542 (64.39%), Postives = 419/542 (77.31%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLQRDDLQLALETPTSIFKLPDSLTKDTPQSYSPSRLFLGPFHEL 60
           MA TS TN V+E+ +LL+RD L++ALETPTSIFKLPDS+T   PQ+Y+PSRL LGPFHEL
Sbjct: 1   MAETSETNWVVEVGELLRRDGLRMALETPTSIFKLPDSVT--NPQAYTPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLAVGSDALAWRI 120
             ELHKMEIFKLE AK  KE++QLPELT +V+QLKE EV IRA Y ANLA+ SDALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGANLAIDSDALASRI 120

Query: 121 LIDGLFLLRLLGIISPDDKEKEEKGKNSQL---PYEVELPVFNEQQLYLNLDGNITT-TQ 180
           LIDGLFL++LL I +    EK ++G +  L    +++ELPVFNEQ+LYL L+GN+ T T+
Sbjct: 121 LIDGLFLIQLLQISA---DEKNDEGSSDWLHNRRHKLELPVFNEQELYLKLEGNMATLTR 180

Query: 181 NEIVKDLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFSGSDEEDDMPFE 240
           NEI+KDLLKLENQIPIQ+LKRVLP++  ENL + FYKFCV VSPL L S         F 
Sbjct: 181 NEILKDLLKLENQIPIQVLKRVLPKDFTENLPILFYKFCVFVSPLSLPSAD------KFT 240

Query: 241 LFPDYLRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGGERSSEILVELLNLMANI 300
              D +  F  L EILD SQHL+HF YALL+YQRF AY      RS  + VELLNL+A +
Sbjct: 241 TSNDNV--FLNLSEILDQSQHLLHFFYALLYYQRFLAYMPSQDYRSGSLFVELLNLLAKV 300

Query: 301 LGIGLLKKLLEALGLIQTLIGFIGKVQKSSPFETDSSPRFPSASKLKKVGVKFDAGDGGG 360
           + +G++++L+EA+GLIQ+LIG +GK+  SSPFET++SP FPSA+KL+KVGVKF  GDGGG
Sbjct: 301 VQVGVVQQLVEAVGLIQSLIGMLGKIHHSSPFETETSPGFPSATKLRKVGVKFRGGDGGG 360

Query: 361 FDEASTTLKLPRITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTIL 420
           F++ S TL LP +T+N F EVILRNLVAFEVEAKLNP  LS YVKLM+GLIAT KDV IL
Sbjct: 361 FNDESATLTLPIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVKLMNGLIATTKDVGIL 420

Query: 421 KRAGIITGGRSGDEDDVVKMFKELGN-VVNVSSEGGESRANFKWYMNFEVDIIEKINVYY 480
           KRAGII  GRSGDEDDVVK+FK+LGN VV    +  E R+NF+ +MNFE  I E+I  YY
Sbjct: 421 KRAGIIVSGRSGDEDDVVKLFKDLGNVVVKGGFDSEEGRSNFESFMNFERRIGEEIVKYY 480

Query: 481 ENRWVVKAERFGKRYIVPMFQALLILVAVVLILIVAVRTVCGWFSCPKIMGHSIIHSGYY 538
           E RW+VK ERF KRY+ P+ Q L+ILV VVLILIVA+RTVCGWFSCPKIMGH II S Y 
Sbjct: 481 EGRWMVKGERFVKRYVFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRIISSAYD 529

BLAST of Lag0039701 vs. NCBI nr
Match: XP_023535329.1 (putative UPF0481 protein At3g02645 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 604.4 bits (1557), Expect = 9.8e-169
Identity = 342/566 (60.42%), Postives = 411/566 (72.61%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLQRDDLQLALETPTSIFKLPDSLTKDTPQSYSPSRLFLGPFHEL 60
           MAGTS TN V+E+ +LL+RD L++ALETPTSIFKLPDS+TK+ PQ+YSPSRL LGPFHEL
Sbjct: 1   MAGTSETNWVVEVGELLRRDGLRMALETPTSIFKLPDSVTKENPQAYSPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLAVGSDALAWRI 120
             ELHKMEIFKLE AK  KE++QLPELT +V+QLKE EV IRA Y ANLA+GSDALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGANLAIGSDALASRI 120

Query: 121 LIDGLFLLRLL----------GIISPDDKEKE----------EKGKNSQLP--------- 180
           LIDGLFL+ LL          GI+     E E            G+   +P         
Sbjct: 121 LIDGLFLIWLLQIYADEKNYPGILQGLSTEDELGPKIRPKGVVDGQQVNIPVLPLVGPEG 180

Query: 181 --------------YEVELPVFNEQQLYLNLDGNITT-TQNEIVKDLLKLENQIPIQILK 240
                         Y++ELPVF+EQ+LYL L+GN+ T T+NEI+KDLLKLENQIPIQ+LK
Sbjct: 181 RRRLGSSDRLHNRRYKLELPVFDEQELYLKLEGNMATMTRNEILKDLLKLENQIPIQVLK 240

Query: 241 RVLPRNLVENLHLSFYKFCVSVSPLPLFSGS--DEEDDMPFELFPDYLRSFPKLPEILDG 300
           RVLP++L+ENL + FYKFCV VSPL L S       +D P          F  L EILD 
Sbjct: 241 RVLPKDLIENLPILFYKFCVFVSPLSLPSADKFTTSNDNP----------FLNLSEILDQ 300

Query: 301 SQHLVHFLYALLFYQRFSAYEQRGGERSSEILVELLNLMANILGIGLLKKLLEALGLIQT 360
           SQHL+HFLYALL+YQRF AY      RS  + VELLNL+A ++ +G++++L+EA+GLIQ+
Sbjct: 301 SQHLLHFLYALLYYQRFLAYRPSQDYRSGSLFVELLNLLAKVVQVGIVQQLVEAVGLIQS 360

Query: 361 LIGFIGKVQKSSPFETDSSPRFPSASKLKKVGVKFDAGDGGGFDEASTTLKLPRITMNAF 420
           LIG +GK+  SSPFET++SP  PSA+KL+KVGVKF  GDGGGFD+ S TL LP +T+N F
Sbjct: 361 LIGMLGKIHHSSPFETETSPGLPSATKLRKVGVKFHGGDGGGFDDESATLTLPSVTINDF 420

Query: 421 SEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILKRAGIITGGRSGDEDDVV 480
            EVILRNLVAFEVEAK+NP  LS YV LM+GLIAT KDV ILKRAGII GGRSGDEDDVV
Sbjct: 421 YEVILRNLVAFEVEAKMNPSSLSHYVTLMNGLIATTKDVGILKRAGIIVGGRSGDEDDVV 480

Query: 481 KMFKELGN-VVNVSSEGGESRANFKWYMNFEVDIIEKINVYYENRWVVKAERFGKRYIVP 520
           K+FK+LGN VV    +  E R+NF+ +MNFE  I E+I  YYE+RW+VKAERF KRYI P
Sbjct: 481 KLFKDLGNVVVKGGFDSEEGRSNFESFMNFEGRIGEEIMKYYESRWMVKAERFVKRYIFP 540

BLAST of Lag0039701 vs. NCBI nr
Match: KAG7025194.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 566.2 bits (1458), Expect = 2.9e-157
Identity = 319/525 (60.76%), Postives = 378/525 (72.00%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLQRDDLQLALETPTSIFKLPDSLTKDTPQSYSPSRLFLGPFHEL 60
           MAGTS TN V+E+ +LL+RD L++ALETPTSIF LPDS+TK+ PQ+Y+PSRL LGPFHEL
Sbjct: 1   MAGTSGTNWVVEVGELLRRDGLRMALETPTSIFMLPDSVTKENPQAYTPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLAVGSDALAWRI 120
             ELHKMEIFKLE AK  KE++QLP+LT +V+QLKE EV IRA Y   LA+GSDALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPDLTLMVDQLKELEVPIRASYGTKLAIGSDALASRI 120

Query: 121 LIDGLFLLRLLGIISPDDKEKEEKGKNSQLPYEVELPVFNEQQLYLNLDGNITTTQNEIV 180
           LIDGLFL+RLL I + +        KN Q                               
Sbjct: 121 LIDGLFLIRLLQIFADE--------KNDQ------------------------------- 180

Query: 181 KDLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFSGS--DEEDDMPFELF 240
            DLLKLENQIPIQ+LKRVLP++L+ENL + FYKFCV VSPL L S       DD P    
Sbjct: 181 -DLLKLENQIPIQVLKRVLPKDLIENLPILFYKFCVFVSPLSLPSADKFTTSDDNP---- 240

Query: 241 PDYLRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGGERSSEILVELLNLMANILG 300
                 F  L EILD SQHL+HF YALL+YQRF AY      RS  + VELLNL+A ++ 
Sbjct: 241 ------FLNLSEILDQSQHLLHFFYALLYYQRFLAYMPSQDYRSGSLFVELLNLLAKVVQ 300

Query: 301 IGLLKKLLEALGLIQTLIGFIGKVQKSSPFETDSSPRFPSASKLKKVGVKFDAGDGGGFD 360
           +G++++L+EA+GLIQ+LIG +GK+  SSPFET++SP FPSA+KL+KVGVKF  GDGGGFD
Sbjct: 301 VGVVQQLVEAVGLIQSLIGMLGKIHHSSPFETETSPGFPSATKLRKVGVKFRGGDGGGFD 360

Query: 361 EASTTLKLPRITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILKR 420
           + S TL LP +T+N F EVILRNLVAFEVEAKLNP  LS YV LM+GLIAT KDV ILKR
Sbjct: 361 DESATLTLPIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVTLMNGLIATTKDVGILKR 420

Query: 421 AGIITGGRSGDEDDVVKMFKELGNVVNVSS-EGGESRANFKWYMNFEVDIIEKINVYYEN 480
           AGII GGRSGDEDDVVK+FK+LGNVV     +  E R+NF+ YMNFE  I E+I  YYE+
Sbjct: 421 AGIIVGGRSGDEDDVVKLFKDLGNVVMKGGFDSEEGRSNFESYMNFERRIGEEIMKYYES 475

Query: 481 RWVVKAERFGKRYIVPMFQALLILVAVVLILIVAVRTVCGWFSCP 523
           RW+VKAERF KRYI P+ Q L+ILV VVLILIVA+RTVC     P
Sbjct: 481 RWMVKAERFVKRYIFPVLQGLMILVVVVLILIVAIRTVCELHKTP 475

BLAST of Lag0039701 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.7e-38
Identity = 144/552 (26.09%), Postives = 243/552 (44.02%), Query Frame = 0

Query: 8   NCVLEIDQLLQRDDLQLALETPTSIFKLPDSLTKDTPQSYSPSRLFLGPFHELKPELHKM 67
           N    +D  L+  DL+   E   SIF +P +L    P SY+P R+ +GP+H LKPELH+M
Sbjct: 24  NVQKSLDAELEEHDLE---EVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEM 83

Query: 68  EIFKLENAKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLAVGSDALAWRILIDGLFL 127
           E +KL  A+K + +Y       +VE+L+  E++IRACY   +    + L W + +D  FL
Sbjct: 84  ERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDSSFL 143

Query: 128 LRLLGIISPDDKEKEEKGKNSQLPYEVELPVFNEQQLYLNLDGNITTTQNEIVKDLLKLE 187
           +  L I S                       F + +  +N  G+     NEI++D++ +E
Sbjct: 144 IEFLKIYS-----------------------FRKVETLINRVGH-----NEILRDIMMIE 203

Query: 188 NQIPIQILKRVLPRNL-----VENLHLSFYK-FCVSVSPLPLFSGSDEEDDMPFELFPDY 247
           NQIP+ +L++ L   L      ++L LS     C  +SPL +    D+     F+     
Sbjct: 204 NQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQ----- 263

Query: 248 LRSFPKLPEILDGSQHLVHFLYALLFYQ----------RFSAYEQRGGERSSEILVELLN 307
                          H++ FLY ++  +            +  ++ GG R+   + E+ +
Sbjct: 264 ------------ECNHILDFLYQMIVPRIEEEELEEDDEENRADENGGNRAIRFMDEIKH 323

Query: 308 --------------------LMANILGIGLLKKLLEALGLIQTLIGFIGKVQKSSPFETD 367
                               +++N+ G   LK   + L   Q       + +  S  + +
Sbjct: 324 QFKRVFASRPADLILRFPWRIISNLPGFMALKLSADYLFTRQENEATTTRQESVSILDIE 383

Query: 368 SSP-----RFPSASKLKKVGVKFDAGDGGG-----FDEASTTLKLPRITMNAFSEVILRN 427
             P       PS S L K GV+F     G      FD  S    LP I ++  +E +LRN
Sbjct: 384 KPPLVEELTIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRN 443

Query: 428 LVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILKRAGIITGGRSGDEDDVVKMFKELG 487
           LVA+E      P   + Y +L++G+I + +DV +L+  G++      D+ +  +M+  + 
Sbjct: 444 LVAYEATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQ-EAAEMWNGMS 503

Query: 488 NVVNVSSEGGESRANFKWYMNFEVDIIEKINVYYENRWVVKAERFGKRYIVPMFQALLIL 514
             V ++  G            F    IE +N YY  RW VK  R  + Y+   +Q L  L
Sbjct: 504 KSVRLTKVG------------FLDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFL 514

BLAST of Lag0039701 vs. ExPASy TrEMBL
Match: A0A6J1EHS6 (putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111432682 PE=4 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 6.8e-184
Identity = 351/541 (64.88%), Postives = 421/541 (77.82%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLQRDDLQLALETPTSIFKLPDSLTKDTPQSYSPSRLFLGPFHEL 60
           MAGTS TN V+E+ +LL+RD L++ALETPTSIFKLPDS+TK+ PQ+Y+PSRL LGPFHEL
Sbjct: 1   MAGTSGTNWVVEVGELLRRDGLRMALETPTSIFKLPDSVTKENPQAYTPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLAVGSDALAWRI 120
             ELHKMEIFKLE AK  KE++QLPELT +V+QLKE EV IRA Y   LA+GSDALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGTKLAIGSDALASRI 120

Query: 121 LIDGLFLLRLLGIISPDDKEKEEKGKNSQLPYEVELPVFNEQQLYLNLDGNITT-TQNEI 180
           LIDGLFL+RLL I + +  ++    +     +++ELPVF+EQ+LYL L+GN+ T T+NEI
Sbjct: 121 LIDGLFLIRLLQIFADEKNDQGSSDRLHNRRHKLELPVFDEQELYLKLEGNMATMTRNEI 180

Query: 181 VKDLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFSGS--DEEDDMPFEL 240
           +KDLLKLENQIPIQ+LKRVLP++L+ENL + FYKFCV VSPL L S       +D P   
Sbjct: 181 LKDLLKLENQIPIQVLKRVLPKDLIENLPILFYKFCVFVSPLSLPSADKFTTSNDNP--- 240

Query: 241 FPDYLRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGGERSSEILVELLNLMANIL 300
                  F  L EILD SQHL+HFLYALL+YQRF AY      RS  + VELLNL+A ++
Sbjct: 241 -------FLNLSEILDQSQHLLHFLYALLYYQRFLAYRPSQDYRSGSLFVELLNLLAKVV 300

Query: 301 GIGLLKKLLEALGLIQTLIGFIGKVQKSSPFETDSSPRFPSASKLKKVGVKFDAGDGGGF 360
            +G++++L+EA+GLIQ+LIG +GK+  SSPFET++SP  PSA KL+KVGVKF  GDGGGF
Sbjct: 301 QVGIVQQLVEAVGLIQSLIGMLGKIHHSSPFETETSPGLPSAMKLRKVGVKFHGGDGGGF 360

Query: 361 DEASTTLKLPRITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILK 420
           D+ S TL LP +T+N F EVILRNLVAFEVEAKLNP  LS YV LM+GLIAT KDV ILK
Sbjct: 361 DDESATLTLPIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVTLMNGLIATTKDVGILK 420

Query: 421 RAGIITGGRSGDEDDVVKMFKELGNVVNVSS-EGGESRANFKWYMNFEVDIIEKINVYYE 480
           RAGII GGRSGDEDDVVK+FK+LGNVV     +  E R+NF+ YMNFE  I E+I  YYE
Sbjct: 421 RAGIIVGGRSGDEDDVVKLFKDLGNVVMKGGFDSEEGRSNFESYMNFERRISEEIMKYYE 480

Query: 481 NRWVVKAERFGKRYIVPMFQALLILVAVVLILIVAVRTVCGWFSCPKIMGHSIIHSGYYF 538
           +RW+VKAERF KRYI P+ Q L+ILV VVLILIVA+RTVCGWFSCPKIMGH II S Y  
Sbjct: 481 SRWMVKAERFVKRYIFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRIISSAYDM 531

BLAST of Lag0039701 vs. ExPASy TrEMBL
Match: A0A6J1IEU8 (UPF0481 protein At3g47200-like OS=Cucurbita maxima OX=3661 GN=LOC111472588 PE=4 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 3.6e-177
Identity = 349/542 (64.39%), Postives = 419/542 (77.31%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLQRDDLQLALETPTSIFKLPDSLTKDTPQSYSPSRLFLGPFHEL 60
           MA TS TN V+E+ +LL+RD L++ALETPTSIFKLPDS+T   PQ+Y+PSRL LGPFHEL
Sbjct: 1   MAETSETNWVVEVGELLRRDGLRMALETPTSIFKLPDSVT--NPQAYTPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLAVGSDALAWRI 120
             ELHKMEIFKLE AK  KE++QLPELT +V+QLKE EV IRA Y ANLA+ SDALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGANLAIDSDALASRI 120

Query: 121 LIDGLFLLRLLGIISPDDKEKEEKGKNSQL---PYEVELPVFNEQQLYLNLDGNITT-TQ 180
           LIDGLFL++LL I +    EK ++G +  L    +++ELPVFNEQ+LYL L+GN+ T T+
Sbjct: 121 LIDGLFLIQLLQISA---DEKNDEGSSDWLHNRRHKLELPVFNEQELYLKLEGNMATLTR 180

Query: 181 NEIVKDLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFSGSDEEDDMPFE 240
           NEI+KDLLKLENQIPIQ+LKRVLP++  ENL + FYKFCV VSPL L S         F 
Sbjct: 181 NEILKDLLKLENQIPIQVLKRVLPKDFTENLPILFYKFCVFVSPLSLPSAD------KFT 240

Query: 241 LFPDYLRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGGERSSEILVELLNLMANI 300
              D +  F  L EILD SQHL+HF YALL+YQRF AY      RS  + VELLNL+A +
Sbjct: 241 TSNDNV--FLNLSEILDQSQHLLHFFYALLYYQRFLAYMPSQDYRSGSLFVELLNLLAKV 300

Query: 301 LGIGLLKKLLEALGLIQTLIGFIGKVQKSSPFETDSSPRFPSASKLKKVGVKFDAGDGGG 360
           + +G++++L+EA+GLIQ+LIG +GK+  SSPFET++SP FPSA+KL+KVGVKF  GDGGG
Sbjct: 301 VQVGVVQQLVEAVGLIQSLIGMLGKIHHSSPFETETSPGFPSATKLRKVGVKFRGGDGGG 360

Query: 361 FDEASTTLKLPRITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTIL 420
           F++ S TL LP +T+N F EVILRNLVAFEVEAKLNP  LS YVKLM+GLIAT KDV IL
Sbjct: 361 FNDESATLTLPIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVKLMNGLIATTKDVGIL 420

Query: 421 KRAGIITGGRSGDEDDVVKMFKELGN-VVNVSSEGGESRANFKWYMNFEVDIIEKINVYY 480
           KRAGII  GRSGDEDDVVK+FK+LGN VV    +  E R+NF+ +MNFE  I E+I  YY
Sbjct: 421 KRAGIIVSGRSGDEDDVVKLFKDLGNVVVKGGFDSEEGRSNFESFMNFERRIGEEIVKYY 480

Query: 481 ENRWVVKAERFGKRYIVPMFQALLILVAVVLILIVAVRTVCGWFSCPKIMGHSIIHSGYY 538
           E RW+VK ERF KRY+ P+ Q L+ILV VVLILIVA+RTVCGWFSCPKIMGH II S Y 
Sbjct: 481 EGRWMVKGERFVKRYVFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRIISSAYD 529

BLAST of Lag0039701 vs. ExPASy TrEMBL
Match: A0A6J1IEU4 (putative UPF0481 protein At3g02645 OS=Cucurbita maxima OX=3661 GN=LOC111472585 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 5.5e-109
Identity = 239/534 (44.76%), Postives = 342/534 (64.04%), Query Frame = 0

Query: 6   RTNCVLEIDQLLQRDDLQLALETPTSIFKLPDSLTKDTPQSYSPSRLFLGPFHELKPELH 65
           +TN V++I+QLL  ++LQ AL+TPTSIFKLP S+T   PQ+Y P  + LGP+H  K EL+
Sbjct: 14  KTNWVVQINQLLDPNNLQTALQTPTSIFKLPHSITNGMPQAYFPCHIALGPYHHFKNELY 73

Query: 66  KMEIFKLENAKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLAVGSDALAWRILIDGL 125
           +ME++KL+NA  A E ++LP+L QI   L++F+++IR+CYD  L  G++ LAW +LIDGL
Sbjct: 74  QMELYKLDNAINAIESFKLPQLHQIAADLEQFDIKIRSCYDKFLDFGAEVLAWIMLIDGL 133

Query: 126 FLLRLLGIISPDDKEKEEKGKNSQLPYEVELPVFNEQQLYLNLDGNITTTQNEIVKDLLK 185
           FL++LL I + +  E     ++  LP     P+F EQ+LY +L      TQ+E+V D+LK
Sbjct: 134 FLVQLLAISNHEKDELRTMRQDLYLP--SPFPLFVEQKLYGSLIEANLMTQDELVNDILK 193

Query: 186 LENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFSGSDEEDDMPFELFPDYLR-- 245
           LENQIPI +LK++LP+NL  NLH+ F++FC  VSP  L          P    P ++R  
Sbjct: 194 LENQIPIFVLKKILPQNLSNNLHILFFRFCEVVSPKKL----------PPHDSPQFMRYS 253

Query: 246 SFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRG-GERSSEILVELLNLMANILGIGLL 305
            +  L +IL+ S HL+HFLY L+     S     G        LVELLN +A++L I LL
Sbjct: 254 GYTDLYQILEQSHHLLHFLYLLILDSSLSIDRPVGMALLRGSFLVELLNALASVLQIALL 313

Query: 306 KKLLEALGLIQTLIGFIGKVQKSSPF---ETDSSPRFPSASKLKKVGVKFDAGDGGGFDE 365
           ++L EA+GLIQ+L+G +G+V  SS     + +  P  PSA +L++VGVK + G+   F E
Sbjct: 314 QQLSEAVGLIQSLLGLLGRVGSSSSSTGEDMNGPPLIPSAEQLRRVGVKLE-GNYEDFHE 373

Query: 366 A--------STTLKLPRITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAK 425
           +           LKLP I +NAFSEV+ RNLVAFE   KLNPPC S+YV L+SGLI T K
Sbjct: 374 SISFEKERREPCLKLPTIVINAFSEVVYRNLVAFEGATKLNPPCFSNYVALISGLITTTK 433

Query: 426 DVTILKRAGIITGGRSGDEDDVVKMFKELGNVVNVSSEGGESRANFKWYMNFEVDIIEKI 485
           DV ILK+A II    +G E++VVK+F  L +V+ + +               E+ +++ I
Sbjct: 434 DVKILKKASIIE-SHAGSEEEVVKLFDGLRSVLELKNMA-------------ELQMVKDI 493

Query: 486 NVYYENRWVVKAERFGKRYIVPMFQALLILVAVVLILIVAVRTVCGWFSCPKIM 526
           N YYE+ W VKA+RF ++YI PM + ++++V ++LI +V VRT CGWF C +I+
Sbjct: 494 NSYYESCWKVKAKRFIQKYINPMLKVIVLVVVILLIGVVVVRTFCGWFGCSRIL 520

BLAST of Lag0039701 vs. ExPASy TrEMBL
Match: A0A6J1DUS9 (putative UPF0481 protein At3g02645 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023673 PE=4 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 7.9e-108
Identity = 239/529 (45.18%), Postives = 348/529 (65.78%), Query Frame = 0

Query: 10  VLEIDQLLQRDDLQLALETPTSIFKLPDSLTKDTPQSYSPSRLFLGPFHELKPELHKMEI 69
           +++I QLL+ ++LQ AL+TPTSIFK+ DS+TK+ P++Y P R+ LGP+H  K +LHKM++
Sbjct: 35  LVQISQLLEPNNLQTALQTPTSIFKIADSITKNNPEAYFPRRVALGPYHHFKNDLHKMQL 94

Query: 70  FKLENAKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLAVGSDALAWRILIDGLFLLR 129
           FKL N  KA+E + L EL+ I+++LK  E++IR+C+D  L +GS+ALA  +LIDGLFL++
Sbjct: 95  FKLHNTIKARETFHLQELSHIMDELKHLEIKIRSCFDHFLDIGSEALARIMLIDGLFLIQ 154

Query: 130 LLGIISPDDKEKEEKGKNSQLPYEVELPVFNEQQLYLNL-DGNITTTQNEIVKDLLKLEN 189
           LL +IS D+K+ E +     L      P+F EQQLY +L +GN+  TQ+E VKD+LKLEN
Sbjct: 155 LL-VISTDEKD-ELRSARPDLCLPSPFPLFVEQQLYSHLIEGNL-MTQDEFVKDILKLEN 214

Query: 190 QIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFSGSDEEDDMPFELFPDYLRSFPKL 249
           QIP+ +LK +LP N   NLHL F+KFC  VSP  L        DMP  +    L  +  L
Sbjct: 215 QIPLFVLKTILPENFGNNLHLLFFKFCNFVSPKQL-----PPHDMPDLM---SLSGYTNL 274

Query: 250 PEILDGSQHLVHFLYALLFYQRFSAYEQRG-GERSSEILVELLNLMANILGIGLLKKLLE 309
            +I+D S HL+HFLY L+  +R S  +        +  LVELLN+++++L IG L++L E
Sbjct: 275 SQIIDQSHHLLHFLYLLILDRRLSVDQAVAMALFRTSFLVELLNVLSSVLQIGFLQQLSE 334

Query: 310 ALGLIQTLIGFIGKV---QKSSPFETDSSPRFPSASKLKKVGVKFDAGDGGGFDEA---- 369
           A GL+Q+L G +G+V     SS  + DS P  PSAS+LK+V VKFD G    F E+    
Sbjct: 335 AAGLLQSLFGLLGRVGSSSSSSGEDMDSPPLIPSASELKRVRVKFD-GRYNDFHESMRFE 394

Query: 370 ----STTLKLPRITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTIL 429
                  L++P + +N FSEV+ RNLVAFE   K N    + YV LMSGLI TAKDV IL
Sbjct: 395 QESKKVCLEMPSMAINVFSEVVFRNLVAFEAATKKNATWFAFYVALMSGLITTAKDVKIL 454

Query: 430 KRAGIITGGRSGDEDDVVKMFKELGNVVNVSSEGGESRANFKWYMNFEVDIIEKINVYYE 489
           K+A II     G E++VVK+F  L N++ + ++   S ++ +     E+ I++ IN YYE
Sbjct: 455 KQAEIIE-SHWGSEEEVVKLFDGLRNLLELHNQ-QMSYSSGRIMEMAEIQIVKDINFYYE 514

Query: 490 NRWVVKAERFGKRYIVPMFQALLILVAVVLILIVAVRTVCGWFSCPKIM 526
           + W VKA+RF KR++ P+ + + +++ ++LI++V +RT CGWFSC +++
Sbjct: 515 SCWKVKAKRFIKRFVNPILKVIFLILVILLIVVVVLRTSCGWFSCSRVL 549

BLAST of Lag0039701 vs. ExPASy TrEMBL
Match: A0A6J1EBZ5 (putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111432681 PE=4 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 3.3e-106
Identity = 234/533 (43.90%), Postives = 338/533 (63.41%), Query Frame = 0

Query: 7   TNCVLEIDQLLQRDDLQLALETPTSIFKLPDSLTKDTPQSYSPSRLFLGPFHELKPELHK 66
           TN V++I+QLL  ++LQ AL+TPTSIFKLP+S+T   PQ+Y P  + LGP+H  + EL++
Sbjct: 15  TNWVVQINQLLDPNNLQTALQTPTSIFKLPNSITNSMPQAYFPRHIALGPYHHFQNELYQ 74

Query: 67  MEIFKLENAKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLAVGSDALAWRILIDGLF 126
           ME++KL+NA  A E ++LP+L QI   L++F+++IR+CY+  L  G++ALAW +LIDGLF
Sbjct: 75  MELYKLDNAINAIESFKLPQLHQIAADLEQFDIKIRSCYNKFLDFGAEALAWIMLIDGLF 134

Query: 127 LLRLLGIISPDDKEKEEKGKNSQLPYEVELPVFNEQQLYLNLDGNITTTQNEIVKDLLKL 186
           L+++L I + ++ E     ++  LP     P+F EQ+LY +L      TQ+E+V D+LKL
Sbjct: 135 LVQILAISNDENDELRRMRQDLYLP--SPFPLFVEQKLYCSLIEANLMTQDELVNDILKL 194

Query: 187 ENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFSGSDEEDDMPFELFPDYLR--S 246
           ENQIP  +LK++LP+NL  NLH+ F++FC  VSP  L          P    P ++R   
Sbjct: 195 ENQIPSFVLKKILPQNLSNNLHILFFRFCEVVSPKKL----------PPHDSPQFMRYSG 254

Query: 247 FPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRG-GERSSEILVELLNLMANILGIGLLK 306
           +  L +IL+ S HL+HFLY L+     S     G        LVELLN +A++L I  L+
Sbjct: 255 YTDLYQILEQSHHLLHFLYLLILDSSLSIDRPVGMALLRGSFLVELLNALASVLQIAFLQ 314

Query: 307 KLLEALGLIQTLIGFIGKVQKSSPF---ETDSSPRFPSASKLKKVGVKFDAGDGGGFDEA 366
           +L EA+GLIQ+L+G   +V  SS     + +  P  PSA +L++VGVK + G+   F E+
Sbjct: 315 QLSEAVGLIQSLLGLFRRVGSSSSSIGEDMNGPPLIPSAEQLRRVGVKLE-GNYEDFHES 374

Query: 367 --------STTLKLPRITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKD 426
                    T LK P I +NAFSEV+  NLVAFE   KLNPPC S+YV LMSGLI T KD
Sbjct: 375 ISFEKERRETCLKFPTIVINAFSEVVYSNLVAFEGATKLNPPCFSNYVALMSGLITTIKD 434

Query: 427 VTILKRAGIITGGRSGDEDDVVKMFKELGNVVNVSSEGGESRANFKWYMNFEVDIIEKIN 486
           V ILK A II     G+E++VVK+F  L +V+ + +               E+ +++ IN
Sbjct: 435 VKILKEASIIE-SHIGNEEEVVKLFDGLRSVLELKNMA-------------EIQMVKDIN 494

Query: 487 VYYENRWVVKAERFGKRYIVPMFQALLILVAVVLILIVAVRTVCGWFSCPKIM 526
            YYE+ W VKA+RF ++YI PM + ++++V ++LI +V VRT CGWF C +I+
Sbjct: 495 SYYESCWKVKAKRFIQKYINPMLKVIVLVVVILLIGVVVVRTFCGWFGCLRIL 520

BLAST of Lag0039701 vs. TAIR 10
Match: AT3G02645.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 162.2 bits (409), Expect = 1.2e-39
Identity = 144/552 (26.09%), Postives = 243/552 (44.02%), Query Frame = 0

Query: 8   NCVLEIDQLLQRDDLQLALETPTSIFKLPDSLTKDTPQSYSPSRLFLGPFHELKPELHKM 67
           N    +D  L+  DL+   E   SIF +P +L    P SY+P R+ +GP+H LKPELH+M
Sbjct: 24  NVQKSLDAELEEHDLE---EVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEM 83

Query: 68  EIFKLENAKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLAVGSDALAWRILIDGLFL 127
           E +KL  A+K + +Y       +VE+L+  E++IRACY   +    + L W + +D  FL
Sbjct: 84  ERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDSSFL 143

Query: 128 LRLLGIISPDDKEKEEKGKNSQLPYEVELPVFNEQQLYLNLDGNITTTQNEIVKDLLKLE 187
           +  L I S                       F + +  +N  G+     NEI++D++ +E
Sbjct: 144 IEFLKIYS-----------------------FRKVETLINRVGH-----NEILRDIMMIE 203

Query: 188 NQIPIQILKRVLPRNL-----VENLHLSFYK-FCVSVSPLPLFSGSDEEDDMPFELFPDY 247
           NQIP+ +L++ L   L      ++L LS     C  +SPL +    D+     F+     
Sbjct: 204 NQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQ----- 263

Query: 248 LRSFPKLPEILDGSQHLVHFLYALLFYQ----------RFSAYEQRGGERSSEILVELLN 307
                          H++ FLY ++  +            +  ++ GG R+   + E+ +
Sbjct: 264 ------------ECNHILDFLYQMIVPRIEEEELEEDDEENRADENGGNRAIRFMDEIKH 323

Query: 308 --------------------LMANILGIGLLKKLLEALGLIQTLIGFIGKVQKSSPFETD 367
                               +++N+ G   LK   + L   Q       + +  S  + +
Sbjct: 324 QFKRVFASRPADLILRFPWRIISNLPGFMALKLSADYLFTRQENEATTTRQESVSILDIE 383

Query: 368 SSP-----RFPSASKLKKVGVKFDAGDGGG-----FDEASTTLKLPRITMNAFSEVILRN 427
             P       PS S L K GV+F     G      FD  S    LP I ++  +E +LRN
Sbjct: 384 KPPLVEELTIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRN 443

Query: 428 LVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILKRAGIITGGRSGDEDDVVKMFKELG 487
           LVA+E      P   + Y +L++G+I + +DV +L+  G++      D+ +  +M+  + 
Sbjct: 444 LVAYEATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQ-EAAEMWNGMS 503

Query: 488 NVVNVSSEGGESRANFKWYMNFEVDIIEKINVYYENRWVVKAERFGKRYIVPMFQALLIL 514
             V ++  G            F    IE +N YY  RW VK  R  + Y+   +Q L  L
Sbjct: 504 KSVRLTKVG------------FLDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFL 514

BLAST of Lag0039701 vs. TAIR 10
Match: AT3G50180.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 68.2 bits (165), Expect = 2.3e-11
Identity = 93/448 (20.76%), Postives = 173/448 (38.62%), Query Frame = 0

Query: 2   AGTSRTNCVLEIDQLLQ---RDDLQLALETPTSIFKLPDSLTKDTPQSYSPSRLFLGPFH 61
           +GT R   V+ I   L+   R+D + +      I+K+P  L  +  +SY P  + LGP+H
Sbjct: 148 SGTKRLEWVISIKDKLEQAYRNDDRTSW-GKLCIYKVPHYLHGNDKKSYFPQTVSLGPYH 207

Query: 62  ELKPELHKMEIFKLENAKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLAVGSDALAW 121
             + +   ME  K        ++     +   ++ + E E + RACY+ ++ + S+    
Sbjct: 208 HGRQQTQSMECHKWRAVNMVLKRTN-QGIEVFLDAMIELEEKARACYEGSIVLSSNEFTE 267

Query: 122 RILIDGLFLLRLLGIISPDDKEKEEKGKNS---QLPYEVELPVFNEQQLYLNLDGNITTT 181
            +L+DG F+L LL            +G N    +L Y+   PVF            +  +
Sbjct: 268 MLLLDGCFILELL------------QGVNEGFLKLGYDHNDPVF-----------AVRGS 327

Query: 182 QNEIVKDLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFSGSDEEDDMPF 241
            + I +D++ LENQ+P+ +L R+L             +  V    +PL   ++   +   
Sbjct: 328 MHSIQRDMIMLENQLPLFVLNRLLELQPGTQNQTGLVELVVRFF-IPLMPTAETLTE--- 387

Query: 242 ELFPDYLRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGGERSSEILVELLNLMAN 301
                   S P+   + +G  H +   +  L + R S                       
Sbjct: 388 -------NSPPR--GVSNGELHCLDVFHRSLLFPRSS----------------------- 447

Query: 302 ILGIGLLKKLLEALGLIQTLIGFIGKVQKSSPFETDSSPRFPSASKLKKVGVKFDAGDGG 361
                                   GK   S   +       P+ ++L+  G KF      
Sbjct: 448 ------------------------GKANYSRVADKHLQRVIPTVTELRDAGFKFKLNKTD 507

Query: 362 GFDE---ASTTLKLPRITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKD 421
            F +   ++  L++P + ++  ++ +  NL+AFE     +   ++ Y+  M  LI + +D
Sbjct: 508 RFWDIKFSNGYLEIPGLLIHDGTKSLFLNLIAFEQCHIESSNDITSYIIFMDNLIDSPED 509

Query: 422 VTILKRAGIITGGRSGDEDDVVKMFKEL 441
           ++ L   GII     G   +V  MF +L
Sbjct: 568 ISYLHHCGIIEHS-LGSNSEVADMFNQL 509

BLAST of Lag0039701 vs. TAIR 10
Match: AT3G60470.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 63.9 bits (154), Expect = 4.4e-10
Identity = 124/549 (22.59%), Postives = 220/549 (40.07%), Query Frame = 0

Query: 32  IFKLPDSLTKDTPQSYSPSRLFLGPFH-----------------ELKPELHKMEIFKLEN 91
           I+++PD L +  P++Y+P  L +GP H                 +L   L+  E  K+  
Sbjct: 34  IYRVPDRLRRVNPEAYTPQLLLIGPLHHSLKFQALNSRGDITNTKLMGYLNMEEHKKIYL 93

Query: 92  AKKAKEKYQLPELTQIVEQLKEFEVQIRACYDANLA-VGSDALAWRILIDGLFLLRLLGI 151
           A+ ++       + ++  ++KE E  IRA Y  + A + S      IL D +F++  L +
Sbjct: 94  ARFSERLAGQKTIDELRRRIKEEEDIIRASYSESTAWIESPKFVDMILHDSVFIIEFL-L 153

Query: 152 ISPDDKEKEEKGKNSQLPYEVELPVFNEQQLYLNLDGNITTTQNEIVKDLLKLENQIPIQ 211
            S D    E++G   +  ++   P+F +  L   +D           +DL+ LENQ+P  
Sbjct: 154 RSKDYTLLEDQGSCIKKTWD---PIFEQPCLQTTVD-----------EDLILLENQLPYF 213

Query: 212 ILKRVL--------PRNLVENLHLSFYKFCVSVSPLPLFSGSDEEDDMPFELFPDYLRSF 271
           IL+++         P   +  + +++++F  ++             D  F  F D  R  
Sbjct: 214 ILEKLFDPIVPILRPNQTLRQIVITYFRFQGNIGV-----------DSRFRHFTDLFRCV 273

Query: 272 ------PKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGGERSSEILVELLNLMANILGI 331
                 PK+P      ++   +L  L  +     +  R  E+S     + L  M    G 
Sbjct: 274 RVETLRPKMP------KNKFRYLVGLFRFVHLGTHGSRELEKSRPQEHKALR-MEKTHGS 333

Query: 332 GLLKKL-------------LEALG----------------LIQTLIGFIGKVQKSSPFET 391
           G   K              +E LG                + +T  G   K  K  P   
Sbjct: 334 GEPTKSTFRYIADLFRCVPMETLGSKREEKSRLERLKTHRMEETSHGHGSKKTKELPI-- 393

Query: 392 DSSPRFPSASKLKKVGVKFDA-GDGGGFDEA--STTLKLPRITMNAFSEVILRNLVAFEV 451
                  +A+KL+  GVKF A  D    D    +  LK+P + +   +E+ LRN++A E 
Sbjct: 394 --IKHMYNAAKLQSAGVKFKAVTDEFSIDVTFENGCLKIPCLWVPDDAEITLRNIMALEQ 453

Query: 452 EAKLNPPCLSDYVKLMSGLIATAKDVTILKRAGIITGGRSGDEDDVVKMFKELGNVVNVS 511
                   + ++V  +  LI T KDV +L   GI+     G++  V      +  +VN  
Sbjct: 454 CHYPFKAYVCNFVSFLDFLIDTDKDVDLLMENGILNNW--GEKSSVA-----VAEMVNTL 513

Query: 512 SEG-GESRANFKWYMNFEVDIIEKINVYYENRWVVKAERFGKRYIVPMFQALLILVAVVL 516
             G  ESR       ++   I  ++N YYEN         G++Y   +++    + A +L
Sbjct: 514 FSGVVESR-------SYYAGIASRVNAYYENPVNRSRTILGRQYFGNLWRGTATVAAALL 531

BLAST of Lag0039701 vs. TAIR 10
Match: AT1G67150.2 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 56.2 bits (134), Expect = 9.2e-08
Identity = 54/191 (28.27%), Postives = 86/191 (45.03%), Query Frame = 0

Query: 338 SASKLKKVGVKF-----DAGDGGGFDEASTTLKLPRITMNAFSEVILRNLVAFEVEAKLN 397
           SA+ L  VGV F     +      F+E    L LP    +  S +ILRN++A+E      
Sbjct: 236 SANMLYSVGVDFKVVKREYSLNVSFEEG--CLILPSFPADESSNIILRNVIAYEQCHDPE 295

Query: 398 PPCLSDYVKLMSGLIATAKDVTILKRAGIITGGRSGDEDDVVKMFKELGNVVNVSSEGGE 457
               ++Y+  M+ LI + +DV IL  AG++T G  G    V+KM   L   V +S++   
Sbjct: 296 NAFTTNYINFMNFLITSDEDVAILTSAGVLTNG-VGRSSMVLKMVNNLAIGVLLSNQSQY 355

Query: 458 SRANFKWYMNFEVDIIEKINVYYENRWVVKAERFGKRYIVPMFQALLILVAVVLILIVAV 517
                        DI+EK+N+++ +R      +  K Y   ++     L A+ L+L+   
Sbjct: 356 H------------DIVEKLNIHHSSRRKRIWAKLRKVYFSDLWTTTATLAAISLLLLTLA 411

Query: 518 RTVCGWFSCPK 524
            TV   F   K
Sbjct: 416 GTVASIFQAIK 411

BLAST of Lag0039701 vs. TAIR 10
Match: AT1G67150.3 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 56.2 bits (134), Expect = 9.2e-08
Identity = 54/191 (28.27%), Postives = 86/191 (45.03%), Query Frame = 0

Query: 338 SASKLKKVGVKF-----DAGDGGGFDEASTTLKLPRITMNAFSEVILRNLVAFEVEAKLN 397
           SA+ L  VGV F     +      F+E    L LP    +  S +ILRN++A+E      
Sbjct: 189 SANMLYSVGVDFKVVKREYSLNVSFEEG--CLILPSFPADESSNIILRNVIAYEQCHDPE 248

Query: 398 PPCLSDYVKLMSGLIATAKDVTILKRAGIITGGRSGDEDDVVKMFKELGNVVNVSSEGGE 457
               ++Y+  M+ LI + +DV IL  AG++T G  G    V+KM   L   V +S++   
Sbjct: 249 NAFTTNYINFMNFLITSDEDVAILTSAGVLTNG-VGRSSMVLKMVNNLAIGVLLSNQSQY 308

Query: 458 SRANFKWYMNFEVDIIEKINVYYENRWVVKAERFGKRYIVPMFQALLILVAVVLILIVAV 517
                        DI+EK+N+++ +R      +  K Y   ++     L A+ L+L+   
Sbjct: 309 H------------DIVEKLNIHHSSRRKRIWAKLRKVYFSDLWTTTATLAAISLLLLTLA 364

Query: 518 RTVCGWFSCPK 524
            TV   F   K
Sbjct: 369 GTVASIFQAIK 364