Lag0039699 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0039699
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRetrotransposon protein
Locationchr2: 48511771 .. 48512622 (+)
RNA-Seq ExpressionLag0039699
SyntenyLag0039699
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGGTGGAAATGGTTCGAGGTATGGATACTCTATGTTAGTACACATCGTGAAGATCTCACGATGTCTTTCTGACACGTGTAATGCGATGTGGAAATAGAATTGTTTAGGTGCATTAGACGGTACGTACATAAAGGTTCATGTTAGTGCGGCTGATCGACTAAGATATAGGATGCGTAAGGGTGAGATTGCCAAATGTATTGGGCGTTGTGTTGCTGAAAGGTGAATTCATTTTCGTTATGTCGGGATGGGAAGGTTCGACTGCTGATTCTCATGTACTCAGGGATGTAATATCACGCCCCAACGGACTGATAGTGCCGAAGGGTATGAAACCCACTATCAATCTAAAATAGGAACACTTATGTCATCCTAAGGTATTAACCTTCTTTTTTGAACAGGCTACTACTATCTCTATCATGTCGGGTATCCAAACGCAGAGGGTTTCTTGGCACCTTACAAAGGAGAGCGGTACCACTTAACCGAGTGGCGTGGGGGAGGTAATGCACCTACTACACCAAGAGAGTTCTTCAACATGAAGCATTCTTCTGTACGTAATGTTATCGAGAGAGCATTTGGTGCGCTGAAAGGATGGTGGGCGATACTTCGAGGGAAGTCCTACTACCTTGTTCGGACCCAGTGTCGAATCATAACAGCATGCTGTTTACTCCACAACCTCATCACCCGAGAGATGGGTCTGGATGTTGGGTTGGATGAAGGTGATATTGGTATGTCTGAACCCGTATCTCTCGATGGCGAGAACATTACCTTCATTCAAAGCTCCACTGAATGGACTCAGAAGCGAGATGACCTAGCCAACAGGATGTTCAACGCGTGGGGGAAACATCATTGA

mRNA sequence

ATGGGAGGTGGAAATGGTTCGAGGGTGAGATTGCCAAATGTATTGGGCGTTGTGTTGCTGAAAGGTGAATTCATTTTCGTTATGTCGGGATGGGAAGGTTCGACTGCTGATTCTCATGTACTCAGGGATGTAATATCACGCCCCAACGGACTGATAGTGCCGAAGGGCTACTACTATCTCTATCATGTCGGGTATCCAAACGCAGAGGGTTTCTTGGCACCTTACAAAGGAGAGCGGTACCACTTAACCGAGTGGCGTGGGGGAGGTAATGCACCTACTACACCAAGAGAGTTCTTCAACATGAAGCATTCTTCTGTACGTAATGTTATCGAGAGAGCATTTGGTGCGCTGAAAGGATGGTGGGCGATACTTCGAGGGAAGTCCTACTACCTTGTTCGGACCCAGTGTCGAATCATAACAGCATGCTGTTTACTCCACAACCTCATCACCCGAGAGATGGGTCTGGATGTTGGGTTGGATGAAGGTGATATTGGTATGTCTGAACCCGTATCTCTCGATGGCGAGAACATTACCTTCATTCAAAGCTCCACTGAATGGACTCAGAAGCGAGATGACCTAGCCAACAGGATGTTCAACGCGTGGGGGAAACATCATTGA

Coding sequence (CDS)

ATGGGAGGTGGAAATGGTTCGAGGGTGAGATTGCCAAATGTATTGGGCGTTGTGTTGCTGAAAGGTGAATTCATTTTCGTTATGTCGGGATGGGAAGGTTCGACTGCTGATTCTCATGTACTCAGGGATGTAATATCACGCCCCAACGGACTGATAGTGCCGAAGGGCTACTACTATCTCTATCATGTCGGGTATCCAAACGCAGAGGGTTTCTTGGCACCTTACAAAGGAGAGCGGTACCACTTAACCGAGTGGCGTGGGGGAGGTAATGCACCTACTACACCAAGAGAGTTCTTCAACATGAAGCATTCTTCTGTACGTAATGTTATCGAGAGAGCATTTGGTGCGCTGAAAGGATGGTGGGCGATACTTCGAGGGAAGTCCTACTACCTTGTTCGGACCCAGTGTCGAATCATAACAGCATGCTGTTTACTCCACAACCTCATCACCCGAGAGATGGGTCTGGATGTTGGGTTGGATGAAGGTGATATTGGTATGTCTGAACCCGTATCTCTCGATGGCGAGAACATTACCTTCATTCAAAGCTCCACTGAATGGACTCAGAAGCGAGATGACCTAGCCAACAGGATGTTCAACGCGTGGGGGAAACATCATTGA

Protein sequence

MGGGNGSRVRLPNVLGVVLLKGEFIFVMSGWEGSTADSHVLRDVISRPNGLIVPKGYYYLYHVGYPNAEGFLAPYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAILRGKSYYLVRTQCRIITACCLLHNLITREMGLDVGLDEGDIGMSEPVSLDGENITFIQSSTEWTQKRDDLANRMFNAWGKHH
Homology
BLAST of Lag0039699 vs. NCBI nr
Match: KAA0062747.1 (retrotransposon protein [Cucumis melo var. makuwa] >TYK22546.1 retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 265.8 bits (678), Expect = 3.1e-67
Identity = 127/189 (67.20%), Postives = 144/189 (76.19%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVLRDVISRPNGLIVPKGYYYLYHVGYPNAEGFL 72
           NVLG    KG+F+FV+ GWEGS ADS +LRD ISR NGL VPKGYYYL   GYPNAEGFL
Sbjct: 131 NVLGACDTKGDFVFVLFGWEGSAADSRILRDAISRHNGLKVPKGYYYLCDAGYPNAEGFL 190

Query: 73  APYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAILRGKSYYLV 132
           APY+GERYHL+EWRG  NAPTT REFFNMKHSS RNVIERAFG LKG WAILRGKSYY V
Sbjct: 191 APYRGERYHLSEWRGESNAPTTTREFFNMKHSSSRNVIERAFGLLKGCWAILRGKSYYPV 250

Query: 133 RTQCRIITACCLLHNLITREMGLDVGLDEGDIGMSEPVSLDGENITFIQSSTEWTQKRDD 192
             QCR I ACCLLHNLI REM     +D+ D G S   +  G+ I +I++S EW++ RD 
Sbjct: 251 DVQCRTIMACCLLHNLINREMTNSEIIDDLDEGDSTYATTGGDEINYIEASNEWSEWRDQ 310

Query: 193 LANRMFNAW 202
           LA+ MF+ W
Sbjct: 311 LAHTMFSDW 319

BLAST of Lag0039699 vs. NCBI nr
Match: KAA0048149.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK08105.1 putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 264.6 bits (675), Expect = 7.0e-67
Identity = 124/189 (65.61%), Postives = 142/189 (75.13%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVLRDVISRPNGLIVPKGYYYLYHVGYPNAEGFL 72
           NVL V    G F+F+++GWEGST DS +LRD +SRPNGL V KGYYYL   GYPNAEGFL
Sbjct: 92  NVLDVCDTNGGFVFILAGWEGSTVDSRILRDALSRPNGLKVSKGYYYLRDAGYPNAEGFL 151

Query: 73  APYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAILRGKSYYLV 132
           APY+G+ YHL EWRG  NAP TP+EFFNMKH S RNVIERAFG LKG WAILRGKSYYLV
Sbjct: 152 APYRGQHYHLQEWRGVENAPETPKEFFNMKHCSARNVIERAFGLLKGRWAILRGKSYYLV 211

Query: 133 RTQCRIITACCLLHNLITREMGLDVGLDEGDIGMSEPVSLDGENITFIQSSTEWTQKRDD 192
           + QCR I ACCLLHNLI REM     LD+ D G S   +  G++IT+I+ S EWT+ RD 
Sbjct: 212 QVQCRTIMACCLLHNLINREMSYIEELDDEDDGDSTHATTSGDDITYIEPSNEWTEWRDA 271

Query: 193 LANRMFNAW 202
           LA+ MF  W
Sbjct: 272 LASSMFTEW 280

BLAST of Lag0039699 vs. NCBI nr
Match: XP_031740445.1 (uncharacterized protein LOC116403439 [Cucumis sativus])

HSP 1 Score: 262.7 bits (670), Expect = 2.7e-66
Identity = 122/189 (64.55%), Postives = 147/189 (77.78%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVLRDVISRPNGLIVPKGYYYLYHVGYPNAEGFL 72
           N +GV   KG+FIFV++GWEGS ADS +L+  I+RPNGL VP+GYYYL+  GYPNA+GFL
Sbjct: 134 NFVGVCDTKGDFIFVLAGWEGSAADSRILQVAIARPNGLHVPQGYYYLFDAGYPNAKGFL 193

Query: 73  APYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAILRGKSYYLV 132
           APY+G+RYHL EWRG GNAP T +E+FNMKHS+ RNVIERAFG LKG WAILR KSYYLV
Sbjct: 194 APYRGQRYHLQEWRGAGNAPATAKEYFNMKHSAARNVIERAFGLLKGRWAILRDKSYYLV 253

Query: 133 RTQCRIITACCLLHNLITREMGLDVGLDEGDIGMSEPVSLDGENITFIQSSTEWTQKRDD 192
           + QCR I AC LLHNLI REM     +D+ D G S   ++ G++I F+++S EWTQ RDD
Sbjct: 254 QIQCRTILACFLLHNLIIREMTNVSFVDDVDEGDSTYATIGGDDIQFVENSNEWTQWRDD 313

Query: 193 LANRMFNAW 202
           LA  MFN W
Sbjct: 314 LAGEMFNEW 322

BLAST of Lag0039699 vs. NCBI nr
Match: KAA0068124.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 259.2 bits (661), Expect = 2.9e-65
Identity = 124/192 (64.58%), Postives = 145/192 (75.52%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVLRDVISRPNGLIVPKGYYYLYHVGYPNAEGFL 72
           NVLGV   KG+F++V++ WEGS ADS +LRD +SRPNGL VPKGYYYL   GYPNAEGFL
Sbjct: 150 NVLGVCDRKGDFVYVLADWEGSAADSRILRDALSRPNGLKVPKGYYYLVDAGYPNAEGFL 209

Query: 73  APYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAILRGKSYYLV 132
           APY+G+RYHL EWRG  NAP+T +EFFNMKHSS RNVIERAFG LKG WAILRGKSYY V
Sbjct: 210 APYRGQRYHLQEWRGPENAPSTSKEFFNMKHSSARNVIERAFGVLKGRWAILRGKSYYPV 269

Query: 133 RTQCRIITACCLLHNLITREM---GLDVGLDEGDIGMSEPVSLDGENITFIQSSTEWTQK 192
             QCR I ACCLLHNLI REM    ++  +DE D   S   +   ++I +I++S EW+Q 
Sbjct: 270 EVQCRTILACCLLHNLINREMTNFDIEDNIDEVD---STHATTAADDIHYIETSNEWSQW 329

Query: 193 RDDLANRMFNAW 202
           RDDLA  MF  W
Sbjct: 330 RDDLAEEMFTEW 338

BLAST of Lag0039699 vs. NCBI nr
Match: KAA0065306.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 258.1 bits (658), Expect = 6.6e-65
Identity = 121/189 (64.02%), Postives = 143/189 (75.66%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVLRDVISRPNGLIVPKGYYYLYHVGYPNAEGFL 72
           NVLGV L  GEFIFVM GWEGS +DS VLRD +SR  GL VPKGYYYL   GYPNAEGFL
Sbjct: 125 NVLGVCLQNGEFIFVMPGWEGSASDSRVLRDAVSRLTGLKVPKGYYYLCDAGYPNAEGFL 184

Query: 73  APYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAILRGKSYYLV 132
           APY+G+RYHLTEWR GGN P  P+E FNM+HS  RNVIERAFG+L   W IL+G+SYYLV
Sbjct: 185 APYRGQRYHLTEWR-GGNPPKCPKELFNMRHSFARNVIERAFGSLNCRWTILQGRSYYLV 244

Query: 133 RTQCRIITACCLLHNLITREMGLDVGLDEGDIGMSEPVSLDGENITFIQSSTEWTQKRDD 192
             QC+IITACCLLHNLI REMG +    E  +G  +   ++ ENI F++++  WT+ RD+
Sbjct: 245 DIQCKIITACCLLHNLIHREMGSEATFKEPHLGEGDSSEMNIENINFVETTNVWTEWRDN 304

Query: 193 LANRMFNAW 202
           LAN+MF  W
Sbjct: 305 LANQMFEDW 312

BLAST of Lag0039699 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.7e-07
Identity = 44/142 (30.99%), Postives = 62/142 (43.66%), Query Frame = 0

Query: 24  FIFVMSGWEGSTADSHVLR--------DVISRPNGLIVPKG------YYYLYHVGYPNAE 83
           F+ V++GW GS  D  VL+        +   R NG  +P         Y +   G+P   
Sbjct: 227 FLDVIAGWPGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLP 286

Query: 84  GFLAPYKGERYHLTEWRGGGNAPTT-PREFFNMKHSSVRNVIERAFGALKGWWAILRGKS 143
             L PY+G+             PT+ P+  FN +HS      + A   LK  W I+ G  
Sbjct: 287 WLLTPYQGK-------------PTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVM 346

Query: 144 YYLVRTQC-RIITACCLLHNLI 150
           +   R +  RII  CCLLHN+I
Sbjct: 347 WMPDRNRLPRIIFVCCLLHNII 355

BLAST of Lag0039699 vs. ExPASy Swiss-Prot
Match: Q96MB7 (Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 5.5e-06
Identity = 46/150 (30.67%), Postives = 68/150 (45.33%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVL-RDVISRPNGLIVPKGYYYLYHVGYPNAEGF 72
           N L V  ++G  + V + W GS  D  VL +  +S      + K  + L      ++  F
Sbjct: 175 NCLMVCDIRGTLMTVETNWPGSLQDCAVLQQSSLSSQFEAGMHKDSWLL-----GDSSFF 234

Query: 73  LAPYKGERYHLTEWRGGGNAPTTPREF-FNMKHSSVRNVIERAFGALKGWWAIL---RGK 132
           L  +     H+         P TP E+ +NM HS+  +VIE+ F  L   +  L   +G 
Sbjct: 235 LRTWLMTPLHI---------PETPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLDGSKGA 294

Query: 133 SYYLVRTQCRIITACCLLHNLITREMGLDV 158
             Y       II ACC+LHN I+ E G+DV
Sbjct: 295 LQYSPEKSSHIILACCVLHN-ISLEHGMDV 309

BLAST of Lag0039699 vs. ExPASy Swiss-Prot
Match: Q17QR8 (Putative nuclease HARBI1 OS=Bos taurus OX=9913 GN=HARBI1 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 9.4e-06
Identity = 46/150 (30.67%), Postives = 68/150 (45.33%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVL-RDVISRPNGLIVPKGYYYLYHVGYPNAEGF 72
           N L V  ++G  + V + W GS  D  VL +  +S      + K  + L      ++  F
Sbjct: 175 NCLMVCDIRGALMTVETSWPGSLQDCVVLQQSSLSSQFEAGMHKESWLL-----GDSSFF 234

Query: 73  LAPYKGERYHLTEWRGGGNAPTTPREF-FNMKHSSVRNVIERAFGALKGWWAIL---RGK 132
           L  +     H+         P TP E+ +NM HS+  +VIE+ F  L   +  L   +G 
Sbjct: 235 LRTWLMTPLHI---------PETPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLDGSKGA 294

Query: 133 SYYLVRTQCRIITACCLLHNLITREMGLDV 158
             Y       II ACC+LHN I+ E G+DV
Sbjct: 295 LQYSPEKSSHIILACCVLHN-ISLEHGMDV 309

BLAST of Lag0039699 vs. ExPASy Swiss-Prot
Match: B0BN95 (Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 7.9e-05
Identity = 44/150 (29.33%), Postives = 64/150 (42.67%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVL-RDVISRPNGLIVPKGYYYLYHVGYPNAEGF 72
           N L V  ++G  + V + W GS  D  VL +  +S      +PK  + L    +      
Sbjct: 175 NCLVVCDIRGALMTVETSWPGSLQDCAVLQQSSLSSQFETGMPKDSWLLGDSSFFLHTWL 234

Query: 73  LAPYKGERYHLTEWRGGGNAPTTPREF-FNMKHSSVRNVIERAFGALKGWWAIL---RGK 132
           L P               + P TP E+ +N  HS+  +VIE+    L   +  L   +G 
Sbjct: 235 LTPL--------------HIPETPAEYRYNRAHSATHSVIEKTLRTLCCRFRCLDGSKGA 294

Query: 133 SYYLVRTQCRIITACCLLHNLITREMGLDV 158
             Y       II ACC+LHN I+ E G+DV
Sbjct: 295 LQYSPEKSSHIILACCVLHN-ISLEHGMDV 309

BLAST of Lag0039699 vs. ExPASy TrEMBL
Match: A0A5D3DG22 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold523G00290 PE=3 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.5e-67
Identity = 127/189 (67.20%), Postives = 144/189 (76.19%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVLRDVISRPNGLIVPKGYYYLYHVGYPNAEGFL 72
           NVLG    KG+F+FV+ GWEGS ADS +LRD ISR NGL VPKGYYYL   GYPNAEGFL
Sbjct: 131 NVLGACDTKGDFVFVLFGWEGSAADSRILRDAISRHNGLKVPKGYYYLCDAGYPNAEGFL 190

Query: 73  APYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAILRGKSYYLV 132
           APY+GERYHL+EWRG  NAPTT REFFNMKHSS RNVIERAFG LKG WAILRGKSYY V
Sbjct: 191 APYRGERYHLSEWRGESNAPTTTREFFNMKHSSSRNVIERAFGLLKGCWAILRGKSYYPV 250

Query: 133 RTQCRIITACCLLHNLITREMGLDVGLDEGDIGMSEPVSLDGENITFIQSSTEWTQKRDD 192
             QCR I ACCLLHNLI REM     +D+ D G S   +  G+ I +I++S EW++ RD 
Sbjct: 251 DVQCRTIMACCLLHNLINREMTNSEIIDDLDEGDSTYATTGGDEINYIEASNEWSEWRDQ 310

Query: 193 LANRMFNAW 202
           LA+ MF+ W
Sbjct: 311 LAHTMFSDW 319

BLAST of Lag0039699 vs. ExPASy TrEMBL
Match: A0A5A7U3X6 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G002670 PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.4e-67
Identity = 124/189 (65.61%), Postives = 142/189 (75.13%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVLRDVISRPNGLIVPKGYYYLYHVGYPNAEGFL 72
           NVL V    G F+F+++GWEGST DS +LRD +SRPNGL V KGYYYL   GYPNAEGFL
Sbjct: 92  NVLDVCDTNGGFVFILAGWEGSTVDSRILRDALSRPNGLKVSKGYYYLRDAGYPNAEGFL 151

Query: 73  APYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAILRGKSYYLV 132
           APY+G+ YHL EWRG  NAP TP+EFFNMKH S RNVIERAFG LKG WAILRGKSYYLV
Sbjct: 152 APYRGQHYHLQEWRGVENAPETPKEFFNMKHCSARNVIERAFGLLKGRWAILRGKSYYLV 211

Query: 133 RTQCRIITACCLLHNLITREMGLDVGLDEGDIGMSEPVSLDGENITFIQSSTEWTQKRDD 192
           + QCR I ACCLLHNLI REM     LD+ D G S   +  G++IT+I+ S EWT+ RD 
Sbjct: 212 QVQCRTIMACCLLHNLINREMSYIEELDDEDDGDSTHATTSGDDITYIEPSNEWTEWRDA 271

Query: 193 LANRMFNAW 202
           LA+ MF  W
Sbjct: 272 LASSMFTEW 280

BLAST of Lag0039699 vs. ExPASy TrEMBL
Match: A0A5A7VL29 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold238G00850 PE=3 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 1.4e-65
Identity = 124/192 (64.58%), Postives = 145/192 (75.52%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVLRDVISRPNGLIVPKGYYYLYHVGYPNAEGFL 72
           NVLGV   KG+F++V++ WEGS ADS +LRD +SRPNGL VPKGYYYL   GYPNAEGFL
Sbjct: 150 NVLGVCDRKGDFVYVLADWEGSAADSRILRDALSRPNGLKVPKGYYYLVDAGYPNAEGFL 209

Query: 73  APYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAILRGKSYYLV 132
           APY+G+RYHL EWRG  NAP+T +EFFNMKHSS RNVIERAFG LKG WAILRGKSYY V
Sbjct: 210 APYRGQRYHLQEWRGPENAPSTSKEFFNMKHSSARNVIERAFGVLKGRWAILRGKSYYPV 269

Query: 133 RTQCRIITACCLLHNLITREM---GLDVGLDEGDIGMSEPVSLDGENITFIQSSTEWTQK 192
             QCR I ACCLLHNLI REM    ++  +DE D   S   +   ++I +I++S EW+Q 
Sbjct: 270 EVQCRTILACCLLHNLINREMTNFDIEDNIDEVD---STHATTAADDIHYIETSNEWSQW 329

Query: 193 RDDLANRMFNAW 202
           RDDLA  MF  W
Sbjct: 330 RDDLAEEMFTEW 338

BLAST of Lag0039699 vs. ExPASy TrEMBL
Match: A0A5A7VG45 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1023G00220 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 3.2e-65
Identity = 121/189 (64.02%), Postives = 143/189 (75.66%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVLRDVISRPNGLIVPKGYYYLYHVGYPNAEGFL 72
           NVLGV L  GEFIFVM GWEGS +DS VLRD +SR  GL VPKGYYYL   GYPNAEGFL
Sbjct: 125 NVLGVCLQNGEFIFVMPGWEGSASDSRVLRDAVSRLTGLKVPKGYYYLCDAGYPNAEGFL 184

Query: 73  APYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAILRGKSYYLV 132
           APY+G+RYHLTEWR GGN P  P+E FNM+HS  RNVIERAFG+L   W IL+G+SYYLV
Sbjct: 185 APYRGQRYHLTEWR-GGNPPKCPKELFNMRHSFARNVIERAFGSLNCRWTILQGRSYYLV 244

Query: 133 RTQCRIITACCLLHNLITREMGLDVGLDEGDIGMSEPVSLDGENITFIQSSTEWTQKRDD 192
             QC+IITACCLLHNLI REMG +    E  +G  +   ++ ENI F++++  WT+ RD+
Sbjct: 245 DIQCKIITACCLLHNLIHREMGSEATFKEPHLGEGDSSEMNIENINFVETTNVWTEWRDN 304

Query: 193 LANRMFNAW 202
           LAN+MF  W
Sbjct: 305 LANQMFEDW 312

BLAST of Lag0039699 vs. ExPASy TrEMBL
Match: A0A1S3C570 (putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103496992 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 4.1e-65
Identity = 124/192 (64.58%), Postives = 145/192 (75.52%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVLRDVISRPNGLIVPKGYYYLYHVGYPNAEGFL 72
           NVLGV   KG+F++V+ GWEGS ADS +LRD +SRPN L VPKGYYYL  VGYPNAEGFL
Sbjct: 111 NVLGVCDTKGDFVYVLVGWEGSAADSRILRDALSRPNELKVPKGYYYLVDVGYPNAEGFL 170

Query: 73  APYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAILRGKSYYLV 132
           APY+G+RYHL EWRG  NAP+T +EFFNMKHSS RNVIERAFG LKG WAIL GKSYYLV
Sbjct: 171 APYRGQRYHLQEWRGPENAPSTSKEFFNMKHSSARNVIERAFGVLKGRWAILWGKSYYLV 230

Query: 133 RTQCRIITACCLLHNLITREM---GLDVGLDEGDIGMSEPVSLDGENITFIQSSTEWTQK 192
             QCR I  CCLLHNLI REM    +   +DE D   S   ++  ++I +I++S EW+Q 
Sbjct: 231 EVQCRTILTCCLLHNLINREMTNFDIQDNIDEVD---STHATIATDDIHYIETSNEWSQW 290

Query: 193 RDDLANRMFNAW 202
           RDDLA  MF+ W
Sbjct: 291 RDDLAEEMFSEW 299

BLAST of Lag0039699 vs. TAIR 10
Match: AT5G35695.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41980.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 115.5 bits (288), Expect = 4.9e-26
Identity = 68/187 (36.36%), Postives = 96/187 (51.34%), Query Frame = 0

Query: 24  FIFVMSGWEGSTADSHVLRDVISRPNGLIVPKGYYYLYHVGYPNAEGFLAPYKGERYHLT 83
           FI+V+SGWEGS  DS VL D + +          +YL   G+ N   FLAP++G RYHL 
Sbjct: 25  FIYVLSGWEGSAHDSRVLSDALRK----------FYLVDCGFANRLNFLAPFRGVRYHLQ 84

Query: 84  EWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAILRGKSYYLVRTQCRIITACC 143
           E+ G    P TP E FN++H S+RNVIER FG  K  +AI +    +  + Q  ++  C 
Sbjct: 85  EFAGQRRDPETPHELFNLRHVSLRNVIERIFGIFKSRFAIFKSAPPFSYKKQAGLVLTCA 144

Query: 144 LLHNLITREMGLD-------VGLDEGDIGMSEPVSLDGENITFIQSSTEWTQKRDDLANR 203
            LHN + +E   D       VG +EGD+  +E  ++   N   I +      ++ D  N 
Sbjct: 145 ALHNFLRKECRSDEADFPDEVG-NEGDVVNNEGNAM---NTNEIDNEEPLEAQKQDRENT 195

BLAST of Lag0039699 vs. TAIR 10
Match: AT5G41980.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G43722.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 106.7 bits (265), Expect = 2.3e-23
Identity = 64/209 (30.62%), Postives = 100/209 (47.85%), Query Frame = 0

Query: 5   NGSRVRLPNVLGVVLLKGEFIFVMSGWEGSTADSHVLRDVISRPNGLIVPKGYYYLYHVG 64
           NG+ +   NVL        F +V++GWEGS +D  VL   ++R N L VP+G YY+    
Sbjct: 166 NGNGLLTQNVLAASSFDLRFNYVLAGWEGSASDQQVLNAALTRRNKLQVPQGKYYIVDNK 225

Query: 65  YPNAEGFLAPYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAIL 124
           YPN  GF+APY G            N+    +E FN +H  +   I R FGALK  + IL
Sbjct: 226 YPNLPGFIAPYHGV---------STNSREEAKEMFNERHKLLHRAIHRTFGALKERFPIL 285

Query: 125 RGKSYYLVRTQCRIITACCLLHNLITREMGLDVGL------DEGDIGMSEPVSLDGENIT 184
                Y ++TQ +++ A C LHN +  E   D+           + G    V+L+ E + 
Sbjct: 286 LSAPPYPLQTQVKLVIAACALHNYVRLEKPDDLVFRMFEEETLAEAGEDREVALEEEQVE 345

Query: 185 FI--------QSSTEWTQKRDDLANRMFN 200
            +        +   +  + RD++A+ ++N
Sbjct: 346 IVGQEHGFRPEEVEDSLRLRDEIASELWN 365

BLAST of Lag0039699 vs. TAIR 10
Match: AT1G43722.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G28730.1); Has 924 Blast hits to 912 proteins in 109 species: Archae - 0; Bacteria - 0; Metazoa - 222; Fungi - 31; Plants - 661; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink). )

HSP 1 Score: 67.8 bits (164), Expect = 1.2e-11
Identity = 39/108 (36.11%), Postives = 58/108 (53.70%), Query Frame = 0

Query: 13  NVLGVVLLKGEFIFVMSGWEGSTADSHVLRDVISRPNGL-IVPKGYYYLYHVGYPNAEGF 72
           N++ +  LK  F ++ +G  GS  D+ VL+      +   + P   YYL   GYPN +G 
Sbjct: 207 NIMAICDLKMLFTYIWNGAPGSCYDTAVLQIAQQSDSEFPLPPSEKYYLVDSGYPNKQGL 266

Query: 73  LAPYKGE-----RYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAF 115
           LAPY+       RYH++++  G   P    E FN  H+S+R+VIER F
Sbjct: 267 LAPYRSSRNRVVRYHMSQFYYGPR-PRNKHELFNQCHTSLRSVIERTF 313

BLAST of Lag0039699 vs. TAIR 10
Match: AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 65.1 bits (157), Expect = 7.6e-11
Identity = 43/137 (31.39%), Postives = 63/137 (45.99%), Query Frame = 0

Query: 14  VLGVVLLKGEFIFVMSGWEGSTADSHVLRD--VISRPNGLIVPKGYYYLYHVGYPNAEGF 73
           +  VV  KG F  +  GW GS  D  VL    +  R N   + KG +     G+P  +  
Sbjct: 324 IQAVVNPKGVFTDLCIGWPGSMPDDKVLEKSLLYQRANNGGLLKGMWVAGGPGHPLLDWV 383

Query: 74  LAPYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGALKGWWAILRGKSYYL 133
           L PY  +              T  +  FN K S V+ V + AFG LKG WA L+ ++   
Sbjct: 384 LVPYTQQNL------------TWTQHAFNEKMSEVQGVAKEAFGRLKGRWACLQKRTEVK 443

Query: 134 VRTQCRIITACCLLHNL 149
           ++    ++ ACC+LHN+
Sbjct: 444 LQDLPTVLGACCVLHNI 448

BLAST of Lag0039699 vs. TAIR 10
Match: AT4G10890.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2439 (InterPro:IPR018838); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G43722.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 62.4 bits (150), Expect = 4.9e-10
Identity = 30/67 (44.78%), Postives = 40/67 (59.70%), Query Frame = 0

Query: 58  YYLYHVGYPNAEGFLAPYKGERYHLTEWRGGGNAPTTPREFFNMKHSSVRNVIERAFGAL 117
           YYL +  YP   G+L P++   YHL ++ G G  P T +E FN KH  +R+VI+R FG  
Sbjct: 95  YYLVNSVYPTTTGYLGPHRRILYHLGQF-GRGGPPVTVQELFNRKHLDLRSVIDRTFGVW 154

Query: 118 KGWWAIL 125
           K  W IL
Sbjct: 155 KAKWRIL 160

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0062747.13.1e-6767.20retrotransposon protein [Cucumis melo var. makuwa] >TYK22546.1 retrotransposon p... [more]
KAA0048149.17.0e-6765.61putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK08105.1 putative nucleas... [more]
XP_031740445.12.7e-6664.55uncharacterized protein LOC116403439 [Cucumis sativus][more]
KAA0068124.12.9e-6564.58retrotransposon protein [Cucumis melo var. makuwa][more]
KAA0065306.16.6e-6564.02retrotransposon protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9M2U31.7e-0730.99Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1[more]
Q96MB75.5e-0630.67Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1[more]
Q17QR89.4e-0630.67Putative nuclease HARBI1 OS=Bos taurus OX=9913 GN=HARBI1 PE=2 SV=1[more]
B0BN957.9e-0529.33Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DG221.5e-6767.20Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7U3X63.4e-6765.61Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A5A7VL291.4e-6564.58Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5A7VG453.2e-6564.02Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A1S3C5704.1e-6564.58putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103496992 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G35695.14.9e-2636.36CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... [more]
AT5G41980.12.3e-2330.62CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... [more]
AT1G43722.11.2e-1136.11unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G12010.17.6e-1131.39unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, ... [more]
AT4G10890.14.9e-1044.78unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2439... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027806Harbinger transposase-derived nuclease domainPFAMPF13359DDE_Tnp_4coord: 13..147
e-value: 1.1E-18
score: 67.4
NoneNo IPR availablePANTHERPTHR22930UNCHARACTERIZEDcoord: 13..170
NoneNo IPR availablePANTHERPTHR22930:SF211NUCLEASE HARBI1-RELATEDcoord: 13..170

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0039699.1Lag0039699.1mRNA