Lag0039680 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0039680 vs. NCBI nr
Match: XP_022973976.1 (GDSL esterase/lipase At1g71691-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 640.6 bits (1651), Expect = 1.0e-179
Identity = 329/435 (75.63%), Postives = 350/435 (80.46%), Query Frame = 0

Query: 1   MAELRLSSILLAVLVMVIMLAAAAS-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNN 60
           MAELR +S+LL VLV++++ AAAA+ GQNGST GGGG  RPAMVPAMFIFGDSLIDNGNN
Sbjct: 1   MAELRFTSMLLPVLVLLLVAAAAAAFGQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNN 60

Query: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSLFLSKIASLNSEANELARR 120
           NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEI                        
Sbjct: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEI------------------------ 120

Query: 121 KRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAELLGLPLIPPNTAPASGPL 180
                                                   A+LLGLPLIPP+ APA+GPL
Sbjct: 121 ----------------------------------------AKLLGLPLIPPSAAPATGPL 180

Query: 181 RGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLDQITGNLGAATVAPVVAGSIFFI 240
           RGLNYASAASGILDITGRNFIGRIPFN+QI NFENTLDQITGNLGAATVAPVVA  IFF+
Sbjct: 181 RGLNYASAASGILDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFFV 240

Query: 241 GMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQHYTQQLTKLYNLGARKFIIPGIGTMGC 300
           GMGSNDYLNNYLMPNYPTRSQY SRQFADLLTQ YTQQL++LYNLGARKFIIPGIGTM C
Sbjct: 241 GMGSNDYLNNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMEC 300

Query: 301 IPNILARTADGRCSEEVSQLVRDFNANVRTMIDNLNANLPGSRFTYVDIARMNEDILANP 360
           IPN+LAR++DGRCSEEVSQLVRDFNANVR MI NLNANLPGSRFTYV+IARMN DILANP
Sbjct: 301 IPNVLARSSDGRCSEEVSQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANP 360

Query: 361 RAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG 420
           RAYGFTV DRGCCGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG
Sbjct: 361 RAYGFTVADRGCCGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG 369

Query: 421 GPTVAYPFNIQQLVD 435
             +VAYPFNIQQL +
Sbjct: 421 DMSVAYPFNIQQLAN 369

BLAST of Lag0039680 vs. NCBI nr
Match: XP_022925467.1 (GDSL esterase/lipase At1g71691-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 639.8 bits (1649), Expect = 1.7e-179
Identity = 329/435 (75.63%), Postives = 349/435 (80.23%), Query Frame = 0

Query: 1   MAELRLSSILLAVLVMVIMLAAAAS-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNN 60
           MAELR SS+LL VLV++++ AAAA+  QNGST GGGG  RPAMVPAMFIFGDSLIDNGNN
Sbjct: 1   MAELRFSSMLLPVLVLLLVAAAAAAFSQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNN 60

Query: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSLFLSKIASLNSEANELARR 120
           NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEI                        
Sbjct: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEI------------------------ 120

Query: 121 KRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAELLGLPLIPPNTAPASGPL 180
                                                   A+LLGLPLIPP+ AP +GPL
Sbjct: 121 ----------------------------------------AKLLGLPLIPPSAAPTTGPL 180

Query: 181 RGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLDQITGNLGAATVAPVVAGSIFFI 240
           RGLNYASAASGILDITGRNFIGRIPFN+QISNFENTLDQITGNLGAATVAPVVA  IFF+
Sbjct: 181 RGLNYASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLGAATVAPVVARCIFFV 240

Query: 241 GMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQHYTQQLTKLYNLGARKFIIPGIGTMGC 300
           GMGSNDYLNNYLMPNYPTRSQY SRQFADLLTQ YTQQL++LYNLGARKFIIPGIGTM C
Sbjct: 241 GMGSNDYLNNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMEC 300

Query: 301 IPNILARTADGRCSEEVSQLVRDFNANVRTMIDNLNANLPGSRFTYVDIARMNEDILANP 360
           IPN+LAR++DGRCSEEVSQLVRDFNANVR MI NLNANLPGSRFTYV+IARMN DILANP
Sbjct: 301 IPNVLARSSDGRCSEEVSQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANP 360

Query: 361 RAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG 420
           RAYGFTV DRGCCGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG
Sbjct: 361 RAYGFTVADRGCCGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG 369

Query: 421 GPTVAYPFNIQQLVD 435
             +VAYPFNIQQL +
Sbjct: 421 DMSVAYPFNIQQLAN 369

BLAST of Lag0039680 vs. NCBI nr
Match: XP_023535504.1 (GDSL esterase/lipase At1g71691-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 638.3 bits (1645), Expect = 5.0e-179
Identity = 328/435 (75.40%), Postives = 348/435 (80.00%), Query Frame = 0

Query: 1   MAELRLSSILLAVLVMVIMLAAAAS-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNN 60
           MAELR SS+LL VLV++++ AAAA+  QNGST GGGG  RPAMVPAMFIFGDSLIDNGNN
Sbjct: 1   MAELRFSSMLLPVLVLLLVAAAAAAFSQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNN 60

Query: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSLFLSKIASLNSEANELARR 120
           NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEI                        
Sbjct: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEI------------------------ 120

Query: 121 KRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAELLGLPLIPPNTAPASGPL 180
                                                   A+LLGLPLIPP+ AP +GPL
Sbjct: 121 ----------------------------------------AKLLGLPLIPPSAAPTTGPL 180

Query: 181 RGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLDQITGNLGAATVAPVVAGSIFFI 240
           RGLNYASAASGILDITGRNFIGRIPFN+QI NFENTLDQITGNLGAATVAPVVA  IFF+
Sbjct: 181 RGLNYASAASGILDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFFV 240

Query: 241 GMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQHYTQQLTKLYNLGARKFIIPGIGTMGC 300
           GMGSNDYLNNYLMPNYPTRSQY SRQFADLLTQ YTQQL++LYNLGARKFIIPGIGTM C
Sbjct: 241 GMGSNDYLNNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMEC 300

Query: 301 IPNILARTADGRCSEEVSQLVRDFNANVRTMIDNLNANLPGSRFTYVDIARMNEDILANP 360
           IPN+LAR++DGRCSEEVSQLVRDFNANVR MI NLNANLPGSRFTYV+IARMN DILANP
Sbjct: 301 IPNVLARSSDGRCSEEVSQLVRDFNANVRVMIGNLNANLPGSRFTYVNIARMNGDILANP 360

Query: 361 RAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG 420
           RAYGFTV DRGCCGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG
Sbjct: 361 RAYGFTVADRGCCGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG 369

Query: 421 GPTVAYPFNIQQLVD 435
             +VAYPFNIQQL +
Sbjct: 421 DMSVAYPFNIQQLAN 369

BLAST of Lag0039680 vs. NCBI nr
Match: KAG6592374.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 634.4 bits (1635), Expect = 7.2e-178
Identity = 326/435 (74.94%), Postives = 347/435 (79.77%), Query Frame = 0

Query: 1   MAELRLSSILLAVLVMVIMLAAAAS-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNN 60
           MAELR SS+LL VLV++++ AAAA+  QNGST GGGG  RPAMVPAMFIFGDSLIDNGNN
Sbjct: 60  MAELRFSSMLLPVLVLLLVAAAAAAFSQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNN 119

Query: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSLFLSKIASLNSEANELARR 120
           NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEI                        
Sbjct: 120 NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEI------------------------ 179

Query: 121 KRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAELLGLPLIPPNTAPASGPL 180
                                                   A+LLGLPLIPP+ AP +GPL
Sbjct: 180 ----------------------------------------AKLLGLPLIPPSAAPTTGPL 239

Query: 181 RGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLDQITGNLGAATVAPVVAGSIFFI 240
           RGLNYASAASGILDITGRNFIGRIPFN+QI NFENTLDQITGNLGAATVAPVVA  IFF+
Sbjct: 240 RGLNYASAASGILDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFFV 299

Query: 241 GMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQHYTQQLTKLYNLGARKFIIPGIGTMGC 300
           GMGSNDYLNNYLMPNYPTRSQY SRQFADLLTQ YTQQL++LYNLGARKFIIPGIGTM C
Sbjct: 300 GMGSNDYLNNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMEC 359

Query: 301 IPNILARTADGRCSEEVSQLVRDFNANVRTMIDNLNANLPGSRFTYVDIARMNEDILANP 360
           IPN+LAR++DGRCSEEV QLVRDFNANVR MI NLNANLPGSRFTYV+IARMN DILANP
Sbjct: 360 IPNVLARSSDGRCSEEVRQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANP 419

Query: 361 RAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG 420
           RAYGFTV DRGCCGIGRNRGQITCLP+QMPCLNRD+YVFWDAFHPTERVNIIMARRAFNG
Sbjct: 420 RAYGFTVADRGCCGIGRNRGQITCLPYQMPCLNRDRYVFWDAFHPTERVNIIMARRAFNG 428

Query: 421 GPTVAYPFNIQQLVD 435
             +VAYPFNIQQL +
Sbjct: 480 DMSVAYPFNIQQLAN 428

BLAST of Lag0039680 vs. NCBI nr
Match: XP_022141840.1 (GDSL esterase/lipase At1g71691-like isoform X1 [Momordica charantia])

HSP 1 Score: 625.2 bits (1611), Expect = 4.4e-175
Identity = 321/434 (73.96%), Postives = 341/434 (78.57%), Query Frame = 0

Query: 1   MAELRLSSILLAVLVMVIMLAAAASGQNGSTY--GGGGGMRPAMVPAMFIFGDSLIDNGN 60
           MAELRLSS ++AVL MVI+LAAAA GQN STY  GGGGG R  MVPAMFIFGDSLIDNGN
Sbjct: 1   MAELRLSS-MVAVLAMVILLAAAAPGQNESTYGGGGGGGWRRPMVPAMFIFGDSLIDNGN 60

Query: 61  NNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSLFLSKIASLNSEANELAR 120
           NNNLPTFARANYFPYGIDF  GPTGRFSNGYTIVDEI                       
Sbjct: 61  NNNLPTFARANYFPYGIDFVGGPTGRFSNGYTIVDEI----------------------- 120

Query: 121 RKRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAELLGLPLIPPNTAPASGP 180
                                                    A+LLGLPLIPP+TAP  GP
Sbjct: 121 -----------------------------------------AKLLGLPLIPPSTAPVMGP 180

Query: 181 LRGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLDQITGNLGAATVAPVVAGSIFF 240
           LRGLNYASAASGILDITGRNFIGRIPFN+QI NFENTLDQITGNLGAATVAPVVA  IFF
Sbjct: 181 LRGLNYASAASGILDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFF 240

Query: 241 IGMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQHYTQQLTKLYNLGARKFIIPGIGTMG 300
           +GMGSNDYLNNYLMPNYPTR QYTSRQFADLL Q YTQQLT+LYNLGARKF+IPGIG MG
Sbjct: 241 VGMGSNDYLNNYLMPNYPTRFQYTSRQFADLLVQRYTQQLTRLYNLGARKFVIPGIGIMG 300

Query: 301 CIPNILARTADGRCSEEVSQLVRDFNANVRTMIDNLNANLPGSRFTYVDIARMNEDILAN 360
           CIPN+LAR++DGRC EEV+QL RDFNANVRTMIDNLN NLPGSRFTY++IAR+ +DILAN
Sbjct: 301 CIPNMLARSSDGRCLEEVNQLGRDFNANVRTMIDNLNTNLPGSRFTYINIARLFQDILAN 360

Query: 361 PRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFN 420
           P AYGFTV D GCCGIGRNRGQITCLPFQMPCLNR+QYVFWDAFHPT+RVNIIMARRAFN
Sbjct: 361 PMAYGFTVADHGCCGIGRNRGQITCLPFQMPCLNREQYVFWDAFHPTQRVNIIMARRAFN 369

Query: 421 GGPTVAYPFNIQQL 433
           G  TVAYPFNIQ+L
Sbjct: 421 GDQTVAYPFNIQRL 369

BLAST of Lag0039680 vs. ExPASy Swiss-Prot
Match: Q9SF78 (GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana OX=3702 GN=At1g71691 PE=2 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 3.8e-129
Identity = 231/393 (58.78%), Postives = 271/393 (68.96%), Query Frame = 0

Query: 42  MVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSL 101
           +VPA+F+FGDSLIDNGNNNN+P+FA+ANYFPYGIDF  GPTGRF NG T+VD I      
Sbjct: 52  IVPALFVFGDSLIDNGNNNNIPSFAKANYFPYGIDFNGGPTGRFCNGLTMVDGI------ 111

Query: 102 FLSKIASLNSEANELARRKRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAE 161
                                                                     A+
Sbjct: 112 ----------------------------------------------------------AQ 171

Query: 162 LLGLPLIPP-NTAPASGPLRGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLDQIT 221
           LLGLPLIP  + A     LRG+NYASAA+GIL  TG NF+GRIPF++QI NFE TLDQ+ 
Sbjct: 172 LLGLPLIPAYSEATGDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQVA 231

Query: 222 GNL-GAATVAPVVAGSIFFIGMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQHYTQQLT 281
               GA  +A  V  S+FFIGMGSNDYLNNYLMPN+PTR+QY S+QF DLL QHYT QLT
Sbjct: 232 SKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQHYTDQLT 291

Query: 282 KLYNLGARKFIIPGIGTMGCIPNILARTADGRCSEEVSQLVRDFNANVRTMIDNLNANLP 341
           +LYNLG RKF++ G+G MGCIP+ILA+  DG+CSEEV+QLV  FN NV+TMI NLN NLP
Sbjct: 292 RLYNLGGRKFVVAGLGRMGCIPSILAQGNDGKCSEEVNQLVLPFNTNVKTMISNLNQNLP 351

Query: 342 GSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFW 401
            ++F Y+DIA M EDI+AN  AYG T  D+GCCGIG+NRGQITCLPF+ PC NRDQYVFW
Sbjct: 352 DAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQYVFW 380

Query: 402 DAFHPTERVNIIMARRAFNGGPTVAYPFNIQQL 433
           DAFHPTE+VN+IMA++AF G  TVAYP NIQQL
Sbjct: 412 DAFHPTEKVNLIMAKKAFAGDRTVAYPINIQQL 380

BLAST of Lag0039680 vs. ExPASy Swiss-Prot
Match: Q9FVV1 (GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.1e-87
Identity = 173/398 (43.47%), Postives = 223/398 (56.03%), Query Frame = 0

Query: 41  AMVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFS 100
           A VPAMF+ GDSL+D GNNN L T ARAN+ PYGID    PTGRFSNG T +D +     
Sbjct: 37  ARVPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNYQPTGRFSNGLTFIDLL----- 96

Query: 101 LFLSKIASLNSEANELARRKRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPA 160
                                                                      A
Sbjct: 97  -----------------------------------------------------------A 156

Query: 161 ELLGLPLIPPNTAPASGP---LRGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLD 220
            LL +P  PP   P +     L+G+NYASAA+GILD++G N+ GR   N+Q+ N E TL 
Sbjct: 157 RLLEIPSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRFSLNQQMVNLETTLS 216

Query: 221 QITGNLGAATVAPVVAGSIFFIGMGSNDYLNNYLMPN-YPTRSQYTSRQFADLLTQHYTQ 280
           Q+   +        +A S+  +  GSNDY+NNYLMPN Y +  ++    FA+LL   Y +
Sbjct: 217 QLRTMMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSSIRFRPPDFANLLLSQYAR 276

Query: 281 QLTKLYNLGARKFIIPGIGTMGCIPNILAR--TADGRCSEEVSQLVRDFNANVRTMIDNL 340
           QL  LY+LG RK  IPG+  +GCIPN  AR  +   RC + V+Q++  FN  +++++D L
Sbjct: 277 QLLTLYSLGLRKIFIPGVAPLGCIPNQRARGISPPDRCVDSVNQILGTFNQGLKSLVDQL 336

Query: 341 NANLPGSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRD 400
           N   PG+ + Y +      DIL NP AYGF+V DR CCGIGRN+GQITCLP Q PC NR+
Sbjct: 337 NQRSPGAIYVYGNTYSAIGDILNNPAAYGFSVVDRACCGIGRNQGQITCLPLQTPCPNRN 370

Query: 401 QYVFWDAFHPTERVNIIMARRAFNGGPTVAYPFNIQQL 433
           QYVFWDAFHPT+  N I+ARRAF G P+ AYP N+QQ+
Sbjct: 397 QYVFWDAFHPTQTANSILARRAFYGPPSDAYPVNVQQM 370

BLAST of Lag0039680 vs. ExPASy Swiss-Prot
Match: Q9FK75 (GDSL esterase/lipase At5g45670 OS=Arabidopsis thaliana OX=3702 GN=At5g45670 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 6.1e-87
Identity = 185/435 (42.53%), Postives = 243/435 (55.86%), Query Frame = 0

Query: 5   RLSSILLAVLVMVIMLAAAASGQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNNNNLPT 64
           R+S +++ ++V V M+  A S                + P  FIFGDSL+DNGNNN L +
Sbjct: 3   RMSLMIMMIMVAVTMINIAKS--------------DPIAPCYFIFGDSLVDNGNNNQLQS 62

Query: 65  FARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSLFLSKIASLNSEANELARRKRAEW 124
            ARANYFPYGIDFA GPTGRFSNG T VD I                             
Sbjct: 63  LARANYFPYGIDFAAGPTGRFSNGLTTVDVI----------------------------- 122

Query: 125 LASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAELLGLP--LIPPNTAPASGPLRGL 184
                                              A+LLG    + P  +A     LRG+
Sbjct: 123 -----------------------------------AQLLGFEDYITPYASARGQDILRGV 182

Query: 185 NYASAASGILDITGRNFIGRIPFNEQISNFENTLDQITGNLGAATVAP-VVAGSIFFIGM 244
           NYASAA+GI D TGR   GRI F  Q++N  NT+ Q+   LG    A   ++  I+ IG+
Sbjct: 183 NYASAAAGIRDETGRQLGGRIAFAGQVANHVNTVSQVVNILGDQNEASNYLSKCIYSIGL 242

Query: 245 GSNDYLNNYLMPN-YPTRSQYTSRQFADLLTQHYTQQLTKLYNLGARKFIIPGIGTMGCI 304
           GSNDYLNNY MP  Y T +Q++   +AD L   YT+QL  LY  GARKF + G+G +GC 
Sbjct: 243 GSNDYLNNYFMPTFYSTGNQFSPESYADDLVARYTEQLRVLYTNGARKFALIGVGAIGCS 302

Query: 305 PNILARTA-DGR-CSEEVSQLVRDFNANVRTMIDNLNANLPGSRFTYVDIARMNEDILAN 364
           PN LA+ + DGR C E ++   R FN+ + +++D  N N P ++FTY++   + +DI+ N
Sbjct: 303 PNELAQNSRDGRTCDERINSANRIFNSKLISIVDAFNQNTPDAKFTYINAYGIFQDIITN 359

Query: 365 PRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAF- 424
           P  YGF VT+ GCCG+GRN GQITCLP Q PCLNR++YVFWDAFHP E  NI++ RR+F 
Sbjct: 363 PARYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRNEYVFWDAFHPGEAANIVIGRRSFK 359

Query: 425 NGGPTVAYPFNIQQL 433
               + A+P++IQQL
Sbjct: 423 REAASDAHPYDIQQL 359

BLAST of Lag0039680 vs. ExPASy Swiss-Prot
Match: Q93YW8 (GDSL esterase/lipase At4g18970 OS=Arabidopsis thaliana OX=3702 GN=At4g18970 PE=2 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.1e-85
Identity = 176/402 (43.78%), Postives = 229/402 (56.97%), Query Frame = 0

Query: 38  MRPAMVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGM 97
           M   + P  FIFGDSL+D+GNNN L + ARANYFPYGIDF  GPTGRFSNG T VD I  
Sbjct: 21  MGDPIAPCYFIFGDSLVDSGNNNRLTSLARANYFPYGIDFQYGPTGRFSNGKTTVDVI-- 80

Query: 98  WFSLFLSKIASLNSEANELARRKRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLS 157
                                                                       
Sbjct: 81  ------------------------------------------------------------ 140

Query: 158 PPAELLGLP--LIPPNTAPASGPLRGLNYASAASGILDITGRNFIGRIPFNEQISNFENT 217
              ELLG    + P + A     LRG+NYASAA+GI + TGR    RI F  Q++N  NT
Sbjct: 141 --TELLGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNT 200

Query: 218 LDQITGNLGAAT-VAPVVAGSIFFIGMGSNDYLNNYLMP-NYPTRSQYTSRQFADLLTQH 277
           + Q+   LG     A  ++  I+ IG+GSNDYLNNY MP  Y T SQY+   +A+ L   
Sbjct: 201 VSQVVNILGDENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINR 260

Query: 278 YTQQLTKLYNLGARKFIIPGIGTMGCIPNILARTA-DG-RCSEEVSQLVRDFNANVRTMI 337
           YT+QL  +YN GARKF + GIG +GC PN LA+ + DG  C E ++   R FN+ + +++
Sbjct: 261 YTEQLRIMYNNGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLV 320

Query: 338 DNLNANLPGSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCL 397
           D+ N N PG++FTY++   + +D++ANP  YGF VT+ GCCG+GRN GQITCLP Q PCL
Sbjct: 321 DHFNQNTPGAKFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCL 358

Query: 398 NRDQYVFWDAFHPTERVNIIMARRAF-NGGPTVAYPFNIQQL 433
           NRD+YVFWDAFHP E  N+++  R+F     + A+P++IQQL
Sbjct: 381 NRDEYVFWDAFHPGEAANVVIGSRSFQRESASDAHPYDIQQL 358

BLAST of Lag0039680 vs. ExPASy Swiss-Prot
Match: Q9C7N4 (GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.8e-84
Identity = 173/398 (43.47%), Postives = 225/398 (56.53%), Query Frame = 0

Query: 41  AMVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFS 100
           A VP  F+FGDSL+DNGNNN L + AR+NYFPYGIDF  GPTGRFSNG T VD I     
Sbjct: 28  AQVPCFFVFGDSLVDNGNNNGLISIARSNYFPYGIDFG-GPTGRFSNGKTTVDVI----- 87

Query: 101 LFLSKIASLNSEANELARRKRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPA 160
                                                                      A
Sbjct: 88  -----------------------------------------------------------A 147

Query: 161 ELLGLPLIPP--NTAPASGPLRGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLDQ 220
           ELLG     P  NT      L G+NYASAA+GI + TGR    RI F+ Q+ N++ T+ Q
Sbjct: 148 ELLGFNGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQLGQRISFSGQVRNYQTTVSQ 207

Query: 221 ITGNLGAAT-VAPVVAGSIFFIGMGSNDYLNNYLMPN-YPTRSQYTSRQFADLLTQHYTQ 280
           +   LG  T  A  +   I+ +G+GSNDYLNNY MP  Y +  Q+T  Q+A+ L   Y+ 
Sbjct: 208 VVQLLGDETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSRQFTPEQYANDLISRYST 267

Query: 281 QLTKLYNLGARKFIIPGIGTMGCIPNILARTADGR-CSEEVSQLVRDFNANVRTMIDNLN 340
           QL  LYN GARKF + GIG +GC PN LA + DGR C + ++   + FN  +R+++D LN
Sbjct: 268 QLNALYNYGARKFALSGIGAVGCSPNALAGSPDGRTCVDRINSANQIFNNKLRSLVDQLN 327

Query: 341 ANLPGSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQ 400
            N P ++F Y++   + +D++ NP  +GF VT+ GCCGIGRN GQITCLP Q PC +R+ 
Sbjct: 328 NNHPDAKFIYINAYGIFQDMITNPARFGFRVTNAGCCGIGRNAGQITCLPGQRPCRDRNA 360

Query: 401 YVFWDAFHPTERVNIIMARRAFNG-GPTVAYPFNIQQL 433
           YVFWDAFHPTE  N+I+ARR++N    + AYP +I +L
Sbjct: 388 YVFWDAFHPTEAANVIIARRSYNAQSASDAYPMDISRL 360

BLAST of Lag0039680 vs. ExPASy TrEMBL
Match: A0A6J1IA39 (GDSL esterase/lipase At1g71691-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111472592 PE=3 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 4.8e-180
Identity = 329/435 (75.63%), Postives = 350/435 (80.46%), Query Frame = 0

Query: 1   MAELRLSSILLAVLVMVIMLAAAAS-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNN 60
           MAELR +S+LL VLV++++ AAAA+ GQNGST GGGG  RPAMVPAMFIFGDSLIDNGNN
Sbjct: 1   MAELRFTSMLLPVLVLLLVAAAAAAFGQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNN 60

Query: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSLFLSKIASLNSEANELARR 120
           NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEI                        
Sbjct: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEI------------------------ 120

Query: 121 KRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAELLGLPLIPPNTAPASGPL 180
                                                   A+LLGLPLIPP+ APA+GPL
Sbjct: 121 ----------------------------------------AKLLGLPLIPPSAAPATGPL 180

Query: 181 RGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLDQITGNLGAATVAPVVAGSIFFI 240
           RGLNYASAASGILDITGRNFIGRIPFN+QI NFENTLDQITGNLGAATVAPVVA  IFF+
Sbjct: 181 RGLNYASAASGILDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFFV 240

Query: 241 GMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQHYTQQLTKLYNLGARKFIIPGIGTMGC 300
           GMGSNDYLNNYLMPNYPTRSQY SRQFADLLTQ YTQQL++LYNLGARKFIIPGIGTM C
Sbjct: 241 GMGSNDYLNNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMEC 300

Query: 301 IPNILARTADGRCSEEVSQLVRDFNANVRTMIDNLNANLPGSRFTYVDIARMNEDILANP 360
           IPN+LAR++DGRCSEEVSQLVRDFNANVR MI NLNANLPGSRFTYV+IARMN DILANP
Sbjct: 301 IPNVLARSSDGRCSEEVSQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANP 360

Query: 361 RAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG 420
           RAYGFTV DRGCCGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG
Sbjct: 361 RAYGFTVADRGCCGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG 369

Query: 421 GPTVAYPFNIQQLVD 435
             +VAYPFNIQQL +
Sbjct: 421 DMSVAYPFNIQQLAN 369

BLAST of Lag0039680 vs. ExPASy TrEMBL
Match: A0A6J1EBS6 (GDSL esterase/lipase At1g71691-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432760 PE=3 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 8.3e-180
Identity = 329/435 (75.63%), Postives = 349/435 (80.23%), Query Frame = 0

Query: 1   MAELRLSSILLAVLVMVIMLAAAAS-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNN 60
           MAELR SS+LL VLV++++ AAAA+  QNGST GGGG  RPAMVPAMFIFGDSLIDNGNN
Sbjct: 1   MAELRFSSMLLPVLVLLLVAAAAAAFSQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNN 60

Query: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSLFLSKIASLNSEANELARR 120
           NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEI                        
Sbjct: 61  NNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEI------------------------ 120

Query: 121 KRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAELLGLPLIPPNTAPASGPL 180
                                                   A+LLGLPLIPP+ AP +GPL
Sbjct: 121 ----------------------------------------AKLLGLPLIPPSAAPTTGPL 180

Query: 181 RGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLDQITGNLGAATVAPVVAGSIFFI 240
           RGLNYASAASGILDITGRNFIGRIPFN+QISNFENTLDQITGNLGAATVAPVVA  IFF+
Sbjct: 181 RGLNYASAASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLGAATVAPVVARCIFFV 240

Query: 241 GMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQHYTQQLTKLYNLGARKFIIPGIGTMGC 300
           GMGSNDYLNNYLMPNYPTRSQY SRQFADLLTQ YTQQL++LYNLGARKFIIPGIGTM C
Sbjct: 241 GMGSNDYLNNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMEC 300

Query: 301 IPNILARTADGRCSEEVSQLVRDFNANVRTMIDNLNANLPGSRFTYVDIARMNEDILANP 360
           IPN+LAR++DGRCSEEVSQLVRDFNANVR MI NLNANLPGSRFTYV+IARMN DILANP
Sbjct: 301 IPNVLARSSDGRCSEEVSQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANP 360

Query: 361 RAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG 420
           RAYGFTV DRGCCGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG
Sbjct: 361 RAYGFTVADRGCCGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG 369

Query: 421 GPTVAYPFNIQQLVD 435
             +VAYPFNIQQL +
Sbjct: 421 DMSVAYPFNIQQLAN 369

BLAST of Lag0039680 vs. ExPASy TrEMBL
Match: A0A6J1CJ89 (GDSL esterase/lipase At1g71691-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012108 PE=3 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 2.1e-175
Identity = 321/434 (73.96%), Postives = 341/434 (78.57%), Query Frame = 0

Query: 1   MAELRLSSILLAVLVMVIMLAAAASGQNGSTY--GGGGGMRPAMVPAMFIFGDSLIDNGN 60
           MAELRLSS ++AVL MVI+LAAAA GQN STY  GGGGG R  MVPAMFIFGDSLIDNGN
Sbjct: 1   MAELRLSS-MVAVLAMVILLAAAAPGQNESTYGGGGGGGWRRPMVPAMFIFGDSLIDNGN 60

Query: 61  NNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSLFLSKIASLNSEANELAR 120
           NNNLPTFARANYFPYGIDF  GPTGRFSNGYTIVDEI                       
Sbjct: 61  NNNLPTFARANYFPYGIDFVGGPTGRFSNGYTIVDEI----------------------- 120

Query: 121 RKRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAELLGLPLIPPNTAPASGP 180
                                                    A+LLGLPLIPP+TAP  GP
Sbjct: 121 -----------------------------------------AKLLGLPLIPPSTAPVMGP 180

Query: 181 LRGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLDQITGNLGAATVAPVVAGSIFF 240
           LRGLNYASAASGILDITGRNFIGRIPFN+QI NFENTLDQITGNLGAATVAPVVA  IFF
Sbjct: 181 LRGLNYASAASGILDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFF 240

Query: 241 IGMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQHYTQQLTKLYNLGARKFIIPGIGTMG 300
           +GMGSNDYLNNYLMPNYPTR QYTSRQFADLL Q YTQQLT+LYNLGARKF+IPGIG MG
Sbjct: 241 VGMGSNDYLNNYLMPNYPTRFQYTSRQFADLLVQRYTQQLTRLYNLGARKFVIPGIGIMG 300

Query: 301 CIPNILARTADGRCSEEVSQLVRDFNANVRTMIDNLNANLPGSRFTYVDIARMNEDILAN 360
           CIPN+LAR++DGRC EEV+QL RDFNANVRTMIDNLN NLPGSRFTY++IAR+ +DILAN
Sbjct: 301 CIPNMLARSSDGRCLEEVNQLGRDFNANVRTMIDNLNTNLPGSRFTYINIARLFQDILAN 360

Query: 361 PRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFN 420
           P AYGFTV D GCCGIGRNRGQITCLPFQMPCLNR+QYVFWDAFHPT+RVNIIMARRAFN
Sbjct: 361 PMAYGFTVADHGCCGIGRNRGQITCLPFQMPCLNREQYVFWDAFHPTQRVNIIMARRAFN 369

Query: 421 GGPTVAYPFNIQQL 433
           G  TVAYPFNIQ+L
Sbjct: 421 GDQTVAYPFNIQRL 369

BLAST of Lag0039680 vs. ExPASy TrEMBL
Match: A0A6J1IEV3 (GDSL esterase/lipase At1g71691-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472592 PE=3 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.5e-173
Identity = 320/427 (74.94%), Postives = 340/427 (79.63%), Query Frame = 0

Query: 9   ILLAVLVMVIMLAAAAS-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNNNNLPTFAR 68
           +LL VLV++++ AAAA+ GQNGST GGGG  RPAMVPAMFIFGDSLIDNGNNNNLPTFAR
Sbjct: 1   MLLPVLVLLLVAAAAAAFGQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNNNNLPTFAR 60

Query: 69  ANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSLFLSKIASLNSEANELARRKRAEWLAS 128
           ANYFPYGIDFADGPTGRFSNGYTIVDEI                                
Sbjct: 61  ANYFPYGIDFADGPTGRFSNGYTIVDEI-------------------------------- 120

Query: 129 ALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAELLGLPLIPPNTAPASGPLRGLNYASA 188
                                           A+LLGLPLIPP+ APA+GPLRGLNYASA
Sbjct: 121 --------------------------------AKLLGLPLIPPSAAPATGPLRGLNYASA 180

Query: 189 ASGILDITGRNFIGRIPFNEQISNFENTLDQITGNLGAATVAPVVAGSIFFIGMGSNDYL 248
           ASGILDITGRNFIGRIPFN+QI NFENTLDQITGNLGAATVAPVVA  IFF+GMGSNDYL
Sbjct: 181 ASGILDITGRNFIGRIPFNQQIRNFENTLDQITGNLGAATVAPVVARCIFFVGMGSNDYL 240

Query: 249 NNYLMPNYPTRSQYTSRQFADLLTQHYTQQLTKLYNLGARKFIIPGIGTMGCIPNILART 308
           NNYLMPNYPTRSQY SRQFADLLTQ YTQQL++LYNLGARKFIIPGIGTM CIPN+LAR+
Sbjct: 241 NNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMECIPNVLARS 300

Query: 309 ADGRCSEEVSQLVRDFNANVRTMIDNLNANLPGSRFTYVDIARMNEDILANPRAYGFTVT 368
           +DGRCSEEVSQLVRDFNANVR MI NLNANLPGSRFTYV+IARMN DILANPRAY   V 
Sbjct: 301 SDGRCSEEVSQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANPRAY---VA 358

Query: 369 DRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGGPTVAYPF 428
           DRGCCGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG  +VAYPF
Sbjct: 361 DRGCCGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGDMSVAYPF 358

Query: 429 NIQQLVD 435
           NIQQL +
Sbjct: 421 NIQQLAN 358

BLAST of Lag0039680 vs. ExPASy TrEMBL
Match: A0A6J1EF98 (GDSL esterase/lipase At1g71691-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111432760 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 5.7e-173
Identity = 319/427 (74.71%), Postives = 339/427 (79.39%), Query Frame = 0

Query: 9   ILLAVLVMVIMLAAAAS-GQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNNNNLPTFAR 68
           +LL VLV++++ AAAA+  QNGST GGGG  RPAMVPAMFIFGDSLIDNGNNNNLPTFAR
Sbjct: 1   MLLPVLVLLLVAAAAAAFSQNGSTDGGGG--RPAMVPAMFIFGDSLIDNGNNNNLPTFAR 60

Query: 69  ANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSLFLSKIASLNSEANELARRKRAEWLAS 128
           ANYFPYGIDFADGPTGRFSNGYTIVDEI                                
Sbjct: 61  ANYFPYGIDFADGPTGRFSNGYTIVDEI-------------------------------- 120

Query: 129 ALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAELLGLPLIPPNTAPASGPLRGLNYASA 188
                                           A+LLGLPLIPP+ AP +GPLRGLNYASA
Sbjct: 121 --------------------------------AKLLGLPLIPPSAAPTTGPLRGLNYASA 180

Query: 189 ASGILDITGRNFIGRIPFNEQISNFENTLDQITGNLGAATVAPVVAGSIFFIGMGSNDYL 248
           ASGILDITGRNFIGRIPFN+QISNFENTLDQITGNLGAATVAPVVA  IFF+GMGSNDYL
Sbjct: 181 ASGILDITGRNFIGRIPFNQQISNFENTLDQITGNLGAATVAPVVARCIFFVGMGSNDYL 240

Query: 249 NNYLMPNYPTRSQYTSRQFADLLTQHYTQQLTKLYNLGARKFIIPGIGTMGCIPNILART 308
           NNYLMPNYPTRSQY SRQFADLLTQ YTQQL++LYNLGARKFIIPGIGTM CIPN+LAR+
Sbjct: 241 NNYLMPNYPTRSQYNSRQFADLLTQQYTQQLSRLYNLGARKFIIPGIGTMECIPNVLARS 300

Query: 309 ADGRCSEEVSQLVRDFNANVRTMIDNLNANLPGSRFTYVDIARMNEDILANPRAYGFTVT 368
           +DGRCSEEVSQLVRDFNANVR MI NLNANLPGSRFTYV+IARMN DILANPRAY   V 
Sbjct: 301 SDGRCSEEVSQLVRDFNANVRIMIGNLNANLPGSRFTYVNIARMNGDILANPRAY---VA 358

Query: 369 DRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGGPTVAYPF 428
           DRGCCGIGRNRGQITCLP+QMPCLNRDQYVFWDAFHPTERVNIIMARRAFNG  +VAYPF
Sbjct: 361 DRGCCGIGRNRGQITCLPYQMPCLNRDQYVFWDAFHPTERVNIIMARRAFNGDMSVAYPF 358

Query: 429 NIQQLVD 435
           NIQQL +
Sbjct: 421 NIQQLAN 358

BLAST of Lag0039680 vs. TAIR 10
Match: AT1G71691.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 463.0 bits (1190), Expect = 2.7e-130
Identity = 231/393 (58.78%), Postives = 271/393 (68.96%), Query Frame = 0

Query: 42  MVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSL 101
           +VPA+F+FGDSLIDNGNNNN+P+FA+ANYFPYGIDF  GPTGRF NG T+VD I      
Sbjct: 52  IVPALFVFGDSLIDNGNNNNIPSFAKANYFPYGIDFNGGPTGRFCNGLTMVDGI------ 111

Query: 102 FLSKIASLNSEANELARRKRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAE 161
                                                                     A+
Sbjct: 112 ----------------------------------------------------------AQ 171

Query: 162 LLGLPLIPP-NTAPASGPLRGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLDQIT 221
           LLGLPLIP  + A     LRG+NYASAA+GIL  TG NF+GRIPF++QI NFE TLDQ+ 
Sbjct: 172 LLGLPLIPAYSEATGDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQVA 231

Query: 222 GNL-GAATVAPVVAGSIFFIGMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQHYTQQLT 281
               GA  +A  V  S+FFIGMGSNDYLNNYLMPN+PTR+QY S+QF DLL QHYT QLT
Sbjct: 232 SKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQHYTDQLT 291

Query: 282 KLYNLGARKFIIPGIGTMGCIPNILARTADGRCSEEVSQLVRDFNANVRTMIDNLNANLP 341
           +LYNLG RKF++ G+G MGCIP+ILA+  DG+CSEEV+QLV  FN NV+TMI NLN NLP
Sbjct: 292 RLYNLGGRKFVVAGLGRMGCIPSILAQGNDGKCSEEVNQLVLPFNTNVKTMISNLNQNLP 351

Query: 342 GSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFW 401
            ++F Y+DIA M EDI+AN  AYG T  D+GCCGIG+NRGQITCLPF+ PC NRDQYVFW
Sbjct: 352 DAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQYVFW 380

Query: 402 DAFHPTERVNIIMARRAFNGGPTVAYPFNIQQL 433
           DAFHPTE+VN+IMA++AF G  TVAYP NIQQL
Sbjct: 412 DAFHPTEKVNLIMAKKAFAGDRTVAYPINIQQL 380

BLAST of Lag0039680 vs. TAIR 10
Match: AT1G71691.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 396.4 bits (1017), Expect = 3.1e-110
Identity = 188/274 (68.61%), Postives = 221/274 (80.66%), Query Frame = 0

Query: 161 ELLGLPLIPP-NTAPASGPLRGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLDQI 220
           +LLGLPLIP  + A     LRG+NYASAA+GIL  TG NF+GRIPF++QI NFE TLDQ+
Sbjct: 6   QLLGLPLIPAYSEATGDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQV 65

Query: 221 TGNL-GAATVAPVVAGSIFFIGMGSNDYLNNYLMPNYPTRSQYTSRQFADLLTQHYTQQL 280
                GA  +A  V  S+FFIGMGSNDYLNNYLMPN+PTR+QY S+QF DLL QHYT QL
Sbjct: 66  ASKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQHYTDQL 125

Query: 281 TKLYNLGARKFIIPGIGTMGCIPNILARTADGRCSEEVSQLVRDFNANVRTMIDNLNANL 340
           T+LYNLG RKF++ G+G MGCIP+ILA+  DG+CSEEV+QLV  FN NV+TMI NLN NL
Sbjct: 126 TRLYNLGGRKFVVAGLGRMGCIPSILAQGNDGKCSEEVNQLVLPFNTNVKTMISNLNQNL 185

Query: 341 PGSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVF 400
           P ++F Y+DIA M EDI+AN  AYG T  D+GCCGIG+NRGQITCLPF+ PC NRDQYVF
Sbjct: 186 PDAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQYVF 245

Query: 401 WDAFHPTERVNIIMARRAFNGGPTVAYPFNIQQL 433
           WDAFHPTE+VN+IMA++AF G  TVAYP NIQQL
Sbjct: 246 WDAFHPTEKVNLIMAKKAFAGDRTVAYPINIQQL 279

BLAST of Lag0039680 vs. TAIR 10
Match: AT1G71250.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.5e-88
Identity = 173/398 (43.47%), Postives = 223/398 (56.03%), Query Frame = 0

Query: 41  AMVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFS 100
           A VPAMF+ GDSL+D GNNN L T ARAN+ PYGID    PTGRFSNG T +D +     
Sbjct: 37  ARVPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNYQPTGRFSNGLTFIDLL----- 96

Query: 101 LFLSKIASLNSEANELARRKRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPA 160
                                                                      A
Sbjct: 97  -----------------------------------------------------------A 156

Query: 161 ELLGLPLIPPNTAPASGP---LRGLNYASAASGILDITGRNFIGRIPFNEQISNFENTLD 220
            LL +P  PP   P +     L+G+NYASAA+GILD++G N+ GR   N+Q+ N E TL 
Sbjct: 157 RLLEIPSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRFSLNQQMVNLETTLS 216

Query: 221 QITGNLGAATVAPVVAGSIFFIGMGSNDYLNNYLMPN-YPTRSQYTSRQFADLLTQHYTQ 280
           Q+   +        +A S+  +  GSNDY+NNYLMPN Y +  ++    FA+LL   Y +
Sbjct: 217 QLRTMMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSSIRFRPPDFANLLLSQYAR 276

Query: 281 QLTKLYNLGARKFIIPGIGTMGCIPNILAR--TADGRCSEEVSQLVRDFNANVRTMIDNL 340
           QL  LY+LG RK  IPG+  +GCIPN  AR  +   RC + V+Q++  FN  +++++D L
Sbjct: 277 QLLTLYSLGLRKIFIPGVAPLGCIPNQRARGISPPDRCVDSVNQILGTFNQGLKSLVDQL 336

Query: 341 NANLPGSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRD 400
           N   PG+ + Y +      DIL NP AYGF+V DR CCGIGRN+GQITCLP Q PC NR+
Sbjct: 337 NQRSPGAIYVYGNTYSAIGDILNNPAAYGFSVVDRACCGIGRNQGQITCLPLQTPCPNRN 370

Query: 401 QYVFWDAFHPTERVNIIMARRAFNGGPTVAYPFNIQQL 433
           QYVFWDAFHPT+  N I+ARRAF G P+ AYP N+QQ+
Sbjct: 397 QYVFWDAFHPTQTANSILARRAFYGPPSDAYPVNVQQM 370

BLAST of Lag0039680 vs. TAIR 10
Match: AT5G45670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 322.8 bits (826), Expect = 4.3e-88
Identity = 185/435 (42.53%), Postives = 243/435 (55.86%), Query Frame = 0

Query: 5   RLSSILLAVLVMVIMLAAAASGQNGSTYGGGGGMRPAMVPAMFIFGDSLIDNGNNNNLPT 64
           R+S +++ ++V V M+  A S                + P  FIFGDSL+DNGNNN L +
Sbjct: 3   RMSLMIMMIMVAVTMINIAKS--------------DPIAPCYFIFGDSLVDNGNNNQLQS 62

Query: 65  FARANYFPYGIDFADGPTGRFSNGYTIVDEIGMWFSLFLSKIASLNSEANELARRKRAEW 124
            ARANYFPYGIDFA GPTGRFSNG T VD I                             
Sbjct: 63  LARANYFPYGIDFAAGPTGRFSNGLTTVDVI----------------------------- 122

Query: 125 LASALGRGRLYGSGQVGPARWSGLSLGRFLVLSPPAELLGLP--LIPPNTAPASGPLRGL 184
                                              A+LLG    + P  +A     LRG+
Sbjct: 123 -----------------------------------AQLLGFEDYITPYASARGQDILRGV 182

Query: 185 NYASAASGILDITGRNFIGRIPFNEQISNFENTLDQITGNLGAATVAP-VVAGSIFFIGM 244
           NYASAA+GI D TGR   GRI F  Q++N  NT+ Q+   LG    A   ++  I+ IG+
Sbjct: 183 NYASAAAGIRDETGRQLGGRIAFAGQVANHVNTVSQVVNILGDQNEASNYLSKCIYSIGL 242

Query: 245 GSNDYLNNYLMPN-YPTRSQYTSRQFADLLTQHYTQQLTKLYNLGARKFIIPGIGTMGCI 304
           GSNDYLNNY MP  Y T +Q++   +AD L   YT+QL  LY  GARKF + G+G +GC 
Sbjct: 243 GSNDYLNNYFMPTFYSTGNQFSPESYADDLVARYTEQLRVLYTNGARKFALIGVGAIGCS 302

Query: 305 PNILARTA-DGR-CSEEVSQLVRDFNANVRTMIDNLNANLPGSRFTYVDIARMNEDILAN 364
           PN LA+ + DGR C E ++   R FN+ + +++D  N N P ++FTY++   + +DI+ N
Sbjct: 303 PNELAQNSRDGRTCDERINSANRIFNSKLISIVDAFNQNTPDAKFTYINAYGIFQDIITN 359

Query: 365 PRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCLNRDQYVFWDAFHPTERVNIIMARRAF- 424
           P  YGF VT+ GCCG+GRN GQITCLP Q PCLNR++YVFWDAFHP E  NI++ RR+F 
Sbjct: 363 PARYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRNEYVFWDAFHPGEAANIVIGRRSFK 359

Query: 425 NGGPTVAYPFNIQQL 433
               + A+P++IQQL
Sbjct: 423 REAASDAHPYDIQQL 359

BLAST of Lag0039680 vs. TAIR 10
Match: AT4G18970.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 318.5 bits (815), Expect = 8.1e-87
Identity = 176/402 (43.78%), Postives = 229/402 (56.97%), Query Frame = 0

Query: 38  MRPAMVPAMFIFGDSLIDNGNNNNLPTFARANYFPYGIDFADGPTGRFSNGYTIVDEIGM 97
           M   + P  FIFGDSL+D+GNNN L + ARANYFPYGIDF  GPTGRFSNG T VD I  
Sbjct: 21  MGDPIAPCYFIFGDSLVDSGNNNRLTSLARANYFPYGIDFQYGPTGRFSNGKTTVDVI-- 80

Query: 98  WFSLFLSKIASLNSEANELARRKRAEWLASALGRGRLYGSGQVGPARWSGLSLGRFLVLS 157
                                                                       
Sbjct: 81  ------------------------------------------------------------ 140

Query: 158 PPAELLGLP--LIPPNTAPASGPLRGLNYASAASGILDITGRNFIGRIPFNEQISNFENT 217
              ELLG    + P + A     LRG+NYASAA+GI + TGR    RI F  Q++N  NT
Sbjct: 141 --TELLGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNT 200

Query: 218 LDQITGNLGAAT-VAPVVAGSIFFIGMGSNDYLNNYLMP-NYPTRSQYTSRQFADLLTQH 277
           + Q+   LG     A  ++  I+ IG+GSNDYLNNY MP  Y T SQY+   +A+ L   
Sbjct: 201 VSQVVNILGDENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINR 260

Query: 278 YTQQLTKLYNLGARKFIIPGIGTMGCIPNILARTA-DG-RCSEEVSQLVRDFNANVRTMI 337
           YT+QL  +YN GARKF + GIG +GC PN LA+ + DG  C E ++   R FN+ + +++
Sbjct: 261 YTEQLRIMYNNGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLV 320

Query: 338 DNLNANLPGSRFTYVDIARMNEDILANPRAYGFTVTDRGCCGIGRNRGQITCLPFQMPCL 397
           D+ N N PG++FTY++   + +D++ANP  YGF VT+ GCCG+GRN GQITCLP Q PCL
Sbjct: 321 DHFNQNTPGAKFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCL 358

Query: 398 NRDQYVFWDAFHPTERVNIIMARRAF-NGGPTVAYPFNIQQL 433
           NRD+YVFWDAFHP E  N+++  R+F     + A+P++IQQL
Sbjct: 381 NRDEYVFWDAFHPGEAANVVIGSRSFQRESASDAHPYDIQQL 358