Lag0039622 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0039622
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Beta-fructofuranosidase, insoluble isoenzyme CWINV3-like
Location	chr2: 47495847 .. 47496400 (+)
RNA-Seq Expression	Lag0039622
Synteny	Lag0039622

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCGAGGGAGGAGCGACGATGCTTTACAGAAGTGAGGACTTTGTTCGTTGGAATCAATTCAGGTTTCCTCTTTATTCTTCTCGCGATTCTGGAACTTCGGAGTGCCCTGATTTTTACCCTGGCTGCTTAATGGAACGAATGGGGTTGAATTTTCGAGTGGATTTGGGATTGGTGTTAAGTATGTGATGAAAGCTAGTTTTAATTCTAGTGACTATTATACATTGGGAAGTTATGCCCCTGAGAAGAAGAAATTCACTTCTGATTCTGGTTCTGGTTTTGATTTTAGAGGAAGAAATTTAGGGTTAAGATATGATTATGGGAAGTTTTATGCTTCCAAGACGTTTTATGACAGTTCCCAGAAGAGGAGGATTTTGTGGGGTTGGGTTAATGAATCTGATACCCGAGAAGATGATATCAATAAGGGATGGTTTGGACTGCAGGGTGATTTTTTTGTGGGAAAAAACCGCAGGCGACCCGACCCGACCCGGATGGAGCCAATACAGAATCAGGTCGGGTTGGGTCGGTCTGTCCGTACAACCGACCCGACCTGA

mRNA sequence

ATGAGCGAGGGAGGAGCGACGATGCTTTACAGAAGTGAGGACTTTGTTCGTTGGAATCAATTCAGTGGATTTGGGATTGGTGTTAAGTATGTGATGAAAGCTAGTTTTAATTCTAGTGACTATTATACATTGGGAAGTTATGCCCCTGAGAAGAAGAAATTCACTTCTGATTCTGGTTCTGGTTTTGATTTTAGAGGAAGAAATTTAGGGTTAAGATATGATTATGGGAAGTTTTATGCTTCCAAGACGTTTTATGACAGTTCCCAGAAGAGGAGGATTTTGTGGGGTTGGGTTAATGAATCTGATACCCGAGAAGATGATATCAATAAGGGATGGTTTGGACTGCAGGGTGATTTTTTTGTGGGAAAAAACCGCAGGCGACCCGACCCGACCCGGATGGAGCCAATACAGAATCAGGTCGGGTTGGGTCGGTCTGTCCGTACAACCGACCCGACCTGA

Coding sequence (CDS)

ATGAGCGAGGGAGGAGCGACGATGCTTTACAGAAGTGAGGACTTTGTTCGTTGGAATCAATTCAGTGGATTTGGGATTGGTGTTAAGTATGTGATGAAAGCTAGTTTTAATTCTAGTGACTATTATACATTGGGAAGTTATGCCCCTGAGAAGAAGAAATTCACTTCTGATTCTGGTTCTGGTTTTGATTTTAGAGGAAGAAATTTAGGGTTAAGATATGATTATGGGAAGTTTTATGCTTCCAAGACGTTTTATGACAGTTCCCAGAAGAGGAGGATTTTGTGGGGTTGGGTTAATGAATCTGATACCCGAGAAGATGATATCAATAAGGGATGGTTTGGACTGCAGGGTGATTTTTTTGTGGGAAAAAACCGCAGGCGACCCGACCCGACCCGGATGGAGCCAATACAGAATCAGGTCGGGTTGGGTCGGTCTGTCCGTACAACCGACCCGACCTGA

Protein sequence

MSEGGATMLYRSEDFVRWNQFSGFGIGVKYVMKASFNSSDYYTLGSYAPEKKKFTSDSGSGFDFRGRNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDTREDDINKGWFGLQGDFFVGKNRRRPDPTRMEPIQNQVGLGRSVRTTDPT

Homology

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BLAST of Lag0039622 vs. NCBI nr
Match: XP_004142928.3 (beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X1 [Cucumis sativus] >KAE8652051.1 hypothetical protein Csa_018697 [Cucumis sativus])

HSP 1 Score: 201.8 bits (512), Expect = 4.1e-48
Identity = 101/147 (68.71%), Postives = 108/147 (73.47%), Query Frame = 0


Query: 2   SEGGATMLYRSEDFVRWNQF--------------------------------SGFGIGVK 61
           S GGA MLYRSEDFVRW+QF                                SGFG+GVK
Sbjct: 237 SYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVK 296

Query: 62  YVMKASFNSSDYYTLGSYAPEKKKFTSDSGSGFDFRGRNLGLRYDYGKFYASKTFYDSSQ 117
           YVMKASFNSSD+YTLGSY PEK+KFTSD G GFDF+G NLGLRYDYGKFYASKTFYDSSQ
Sbjct: 297 YVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQ 356

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BLAST of Lag0039622 vs. NCBI nr
Match: XP_011649529.2 (beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X2 [Cucumis sativus])

HSP 1 Score: 201.8 bits (512), Expect = 4.1e-48
Identity = 101/147 (68.71%), Postives = 108/147 (73.47%), Query Frame = 0


Query: 2   SEGGATMLYRSEDFVRWNQF--------------------------------SGFGIGVK 61
           S GGA MLYRSEDFVRW+QF                                SGFG+GVK
Sbjct: 234 SYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVK 293

Query: 62  YVMKASFNSSDYYTLGSYAPEKKKFTSDSGSGFDFRGRNLGLRYDYGKFYASKTFYDSSQ 117
           YVMKASFNSSD+YTLGSY PEK+KFTSD G GFDF+G NLGLRYDYGKFYASKTFYDSSQ
Sbjct: 294 YVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQ 353

[Close]

BLAST of Lag0039622 vs. NCBI nr
Match: ALS30215.1 (cell wall invertase 4 [Cucumis sativus])

HSP 1 Score: 201.8 bits (512), Expect = 4.1e-48
Identity = 101/147 (68.71%), Postives = 108/147 (73.47%), Query Frame = 0


Query: 2   SEGGATMLYRSEDFVRWNQF--------------------------------SGFGIGVK 61
           S GGA MLYRSEDFVRW+QF                                SGFG+GVK
Sbjct: 205 SYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVK 264

Query: 62  YVMKASFNSSDYYTLGSYAPEKKKFTSDSGSGFDFRGRNLGLRYDYGKFYASKTFYDSSQ 117
           YVMKASFNSSD+YTLGSY PEK+KFTSD G GFDF+G NLGLRYDYGKFYASKTFYDSSQ
Sbjct: 265 YVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDFKGINLGLRYDYGKFYASKTFYDSSQ 324

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BLAST of Lag0039622 vs. NCBI nr
Match: XP_008444460.1 (PREDICTED: LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWINV3-like [Cucumis melo])

HSP 1 Score: 199.1 bits (505), Expect = 2.7e-47
Identity = 100/147 (68.03%), Postives = 107/147 (72.79%), Query Frame = 0


Query: 2   SEGGATMLYRSEDFVRWNQF--------------------------------SGFGIGVK 61
           S GGA MLYRSEDFVRW+QF                                SGFG+GVK
Sbjct: 210 SYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVK 269

Query: 62  YVMKASFNSSDYYTLGSYAPEKKKFTSDSGSGFDFRGRNLGLRYDYGKFYASKTFYDSSQ 117
           +VMKASFNS D+YTLGSY PEK+KFTSD G GFDFRG NLGLRYDYGKFYASKTFYDSSQ
Sbjct: 270 HVMKASFNSRDHYTLGSYVPEKEKFTSDHGLGFDFRGINLGLRYDYGKFYASKTFYDSSQ 329

[Close]

BLAST of Lag0039622 vs. NCBI nr
Match: KAA0061001.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like [Cucumis melo var. makuwa])

HSP 1 Score: 198.7 bits (504), Expect = 3.5e-47
Identity = 100/147 (68.03%), Postives = 107/147 (72.79%), Query Frame = 0


Query: 2   SEGGATMLYRSEDFVRWNQF--------------------------------SGFGIGVK 61
           S GGA MLYRSEDFVRW+QF                                SGFG+GVK
Sbjct: 119 SYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVK 178

Query: 62  YVMKASFNSSDYYTLGSYAPEKKKFTSDSGSGFDFRGRNLGLRYDYGKFYASKTFYDSSQ 117
           +VMKASFNS D+YTLGSY PEK+KFTSD G GFDFRG NLGLRYDYGKFYASKTFYDSSQ
Sbjct: 179 HVMKASFNSRDHYTLGSYVPEKEKFTSDYGLGFDFRGINLGLRYDYGKFYASKTFYDSSQ 238

[Close]

BLAST of Lag0039622 vs. ExPASy Swiss-Prot
Match: Q8W4S6 (Beta-fructofuranosidase, insoluble isoenzyme CWINV6 OS=Arabidopsis thaliana OX=3702 GN=CWINV6 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.8e-22
Identity = 56/145 (38.62%), Postives = 81/145 (55.86%), Query Frame = 0


Query: 2   SEGGATMLYRSEDFVRWNQF-------SGFGI------------------------GVKY 61
           +E G  +LYRS+DFV+W ++        G G+                         V++
Sbjct: 181 TEKGMAILYRSDDFVQWTKYPVPLLESEGTGMWECPDFFPVSITGKEGVDTSVNNASVRH 240

Query: 62  VMKASFNSSDYYTLGSYAPEKKKFTSDSGSGFDFRGRNLGLRYDYGKFYASKTFYDSSQK 116
           V+KASF  +D Y +G Y+ E + F++D    ++F   +  LRYD+G FYASK F+DS + 
Sbjct: 241 VLKASFGGNDCYVIGKYSSETEDFSAD----YEFTNTSADLRYDHGTFYASKAFFDSVKN 300

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BLAST of Lag0039622 vs. ExPASy Swiss-Prot
Match: Q39693 (Beta-fructofuranosidase, insoluble isoenzyme 3 OS=Daucus carota OX=4039 GN=INV3 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 6.9e-22
Identity = 59/145 (40.69%), Postives = 77/145 (53.10%), Query Frame = 0


Query: 5   GATMLYRSEDFVRWNQF-------------------------------SGFGIGVKYVMK 64
           G   LYRS+DF +W +                                S  G G+K+V+K
Sbjct: 217 GIAYLYRSKDFKKWKRSPHPIHTKAETGMWECPDFYPVSPRSEDGLDNSKMGRGIKHVLK 276

Query: 65  ASFNSS--DYYTLGSYAPEKKKFTSDSGSGFDFRGRNLGLRYDYGKFYASKTFYDSSQKR 117
            S NS+  +YYT+G Y   +  +  D+ S   +     GLRYDYG FYASKTFYD  +KR
Sbjct: 277 VSLNSTRYEYYTIGRYNRVRDFYVPDNTSVDGW----AGLRYDYGNFYASKTFYDPIKKR 336

[Close]

BLAST of Lag0039622 vs. ExPASy Swiss-Prot
Match: Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.5e-21
Identity = 60/145 (41.38%), Postives = 76/145 (52.41%), Query Frame = 0


Query: 5   GATMLYRSEDFVRWNQ----------------------FSGFGIG---------VKYVMK 64
           G  +L+ S+DFV+WN                       + G  +G         VK+V+K
Sbjct: 223 GLAVLFTSDDFVKWNNTGNPLHSTEGNGIWECPDFFPVYVGKSLGADTSIIGDDVKHVLK 282

Query: 65  ASF--NSSDYYTLGSYAPEKKKFTSDSGSGFDFRGRNLGLRYDYGKFYASKTFYDSSQKR 117
            S      +YYT+G Y  EK  +  D GS       +LGLRYDYGKFYASK+F+D    R
Sbjct: 283 LSLFDTQYEYYTIGRYDIEKDIYVPDEGS----IESDLGLRYDYGKFYASKSFFDDETNR 342

[Close]

BLAST of Lag0039622 vs. ExPASy Swiss-Prot
Match: H2DF88 (Acid beta-fructofuranosidase 2, vacuolar OS=Rosa hybrid cultivar OX=128735 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.5e-21
Identity = 56/150 (37.33%), Postives = 82/150 (54.67%), Query Frame = 0


Query: 1   MSEGGATMLYRSEDFVRWNQFSGF-------------------------------GIGVK 60
           +++ G +++Y ++DF+++ Q  G                                G  VK
Sbjct: 240 VNKTGISLVYDTKDFIKYEQLDGVLHAVPGTGMWECIDFYPVSKTSDKGLDTSQNGADVK 299

Query: 61  YVMKASFNS--SDYYTLGSYAPEKKKFTSDSGSGFDFRGRNLGLRYDYGKFYASKTFYDS 118
           +VMKAS +   +DYY LGSY  +  K+  D+    D     +G+RYDYGKFYASKTFYD 
Sbjct: 300 HVMKASLDDDRNDYYALGSYNEKTGKWVPDN-QKIDV---GIGIRYDYGKFYASKTFYDQ 359

[Close]

BLAST of Lag0039622 vs. ExPASy Swiss-Prot
Match: Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 7.6e-21
Identity = 48/91 (52.75%), Postives = 64/91 (70.33%), Query Frame = 0


Query: 28  VKYVMKASFNSS--DYYTLGSYAPEKKKFTSDSGSGFDFRGRNLGLRYDYGKFYASKTFY 87
           +K+V+K S + +  DYYT+G+Y   K KF  D+G    F+      RYDYGK+YASKTF+
Sbjct: 273 LKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNG----FKMDGTAPRYDYGKYYASKTFF 332

Query: 88  DSSQKRRILWGWVNESDTREDDINKGWFGLQ 117
           DS++ RRILWGW NES + EDD+ KGW G+Q
Sbjct: 333 DSAKNRRILWGWTNESSSVEDDVEKGWSGIQ 359

The following BLAST results are available for this feature:

BLAST of Lag0039622 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142928.3	4.1e-48	68.71	beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X1 [Cucumis sativus]...	[more]
XP_011649529.2	4.1e-48	68.71	beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X2 [Cucumis sativus]	[more]
ALS30215.1	4.1e-48	68.71	cell wall invertase 4 [Cucumis sativus]	[more]
XP_008444460.1	2.7e-47	68.03	PREDICTED: LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWI...	[more]
KAA0061001.1	3.5e-47	68.03	beta-fructofuranosidase, insoluble isoenzyme CWINV3-like [Cucumis melo var. maku...	[more]

BLAST of Lag0039622 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8W4S6	1.8e-22	38.62	Beta-fructofuranosidase, insoluble isoenzyme CWINV6 OS=Arabidopsis thaliana OX=3...	[more]
Q39693	6.9e-22	40.69	Beta-fructofuranosidase, insoluble isoenzyme 3 OS=Daucus carota OX=4039 GN=INV3 ...	[more]
Q70XE6	1.5e-21	41.38	Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1	[more]
H2DF88	1.5e-21	37.33	Acid beta-fructofuranosidase 2, vacuolar OS=Rosa hybrid cultivar OX=128735 PE=2 ...	[more]
Q43866	7.6e-21	52.75	Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023296	Glycosyl hydrolase, five-bladed beta-propellor domain superfamily	GENE3D	2.115.10.20	Glycosyl hydrolase domain; family 43	coord: 1..118 e-value: 1.3E-19 score: 72.9
IPR023296	Glycosyl hydrolase, five-bladed beta-propellor domain superfamily	SUPERFAMILY	75005	Arabinanase/levansucrase/invertase	coord: 3..116
IPR013148	Glycosyl hydrolase family 32, N-terminal	PFAM	PF00251	Glyco_hydro_32N	coord: 29..116 e-value: 1.4E-16 score: 61.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 124..152
None	No IPR available	PANTHER	PTHR31953:SF78	GLYCOSIDE HYDROLASE, FAMILY 32-RELATED	coord: 22..116
None	No IPR available	PANTHER	PTHR31953	BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED	coord: 22..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0039622.1	Lag0039622.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
molecular_function	GO:0016798	hydrolase activity, acting on glycosyl bonds