Lag0039464 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0039464
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Resistance gene-like protein
Location	chr2: 44245770 .. 44246198 (+)
RNA-Seq Expression	Lag0039464
Synteny	Lag0039464

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGGTATTGGTAAAACAACCATTGCTCAATTCGTTTTTTATTGTCTTCTTTCTAAGTTTGACGATAGTTGCTTTCCAACATTATCCAGAGGTGATTCAAAGCAAAGTTTAGTACCATTACAACGTGAAATGCTTTCTAAAATTTTTAGGGAGGATTTTATAATATGGAATGACATTCATGGGGTGGAGATGATTAAAAATCGACTTAGTAGTAGAAAGGTTCTTCTTGTTCTTGTTCTTGATGGGGTTGAAGAGAAAATGCAATTAGAAAAGTTGGTTGGAAGCATTGATTGGTTGGGCCCTGGAAGCAGAGTCATCGTTACAACTAGAAATAAAGAATTACTCTATCAACCAAATAATGTTGAAGTGAAAGAATACAATGTTGAAGAACTAGATCATGATAGTGCTCTCCACAACTCTTTTTGA

mRNA sequence

ATGGGTGGTATTGGTAAAACAACCATTGCTCAATTCGTTTTTTATTGTCTTCTTTCTAAGTTTGACGATAGTTGCTTTCCAACATTATCCAGAGGTGATTCAAAGCAAAGTTTAGTACCATTACAACGTGAAATGCTTTCTAAAATTTTTAGGGAGGATTTTATAATATGGAATGACATTCATGGGGTGGAGATGATTAAAAATCGACTTAGTAGTAGAAAGGTTCTTCTTGTTCTTGTTCTTGATGGGGTTGAAGAGAAAATGCAATTAGAAAAGTTGGTTGGAAGCATTGATTGGTTGGGCCCTGGAAGCAGAGTCATCGTTACAACTAGAAATAAAGAATTACTCTATCAACCAAATAATGTTGAAGTGAAAGAATACAATGTTGAAGAACTAGATCATGATAGTGCTCTCCACAACTCTTTTTGA

Coding sequence (CDS)

ATGGGTGGTATTGGTAAAACAACCATTGCTCAATTCGTTTTTTATTGTCTTCTTTCTAAGTTTGACGATAGTTGCTTTCCAACATTATCCAGAGGTGATTCAAAGCAAAGTTTAGTACCATTACAACGTGAAATGCTTTCTAAAATTTTTAGGGAGGATTTTATAATATGGAATGACATTCATGGGGTGGAGATGATTAAAAATCGACTTAGTAGTAGAAAGGTTCTTCTTGTTCTTGTTCTTGATGGGGTTGAAGAGAAAATGCAATTAGAAAAGTTGGTTGGAAGCATTGATTGGTTGGGCCCTGGAAGCAGAGTCATCGTTACAACTAGAAATAAAGAATTACTCTATCAACCAAATAATGTTGAAGTGAAAGAATACAATGTTGAAGAACTAGATCATGATAGTGCTCTCCACAACTCTTTTTGA

Protein sequence

MGGIGKTTIAQFVFYCLLSKFDDSCFPTLSRGDSKQSLVPLQREMLSKIFREDFIIWNDIHGVEMIKNRLSSRKVLLVLVLDGVEEKMQLEKLVGSIDWLGPGSRVIVTTRNKELLYQPNNVEVKEYNVEELDHDSALHNSF

Homology

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BLAST of Lag0039464 vs. NCBI nr
Match: AGH33850.1 (resistance gene-like protein [Cucumis melo])

HSP 1 Score: 204.5 bits (519), Expect = 6.0e-49
Identity = 106/139 (76.26%), Postives = 116/139 (83.45%), Query Frame = 0


Query: 1   MGGIGKTTIAQFVFYCLLSKFDDSCFPTLSRGDSKQSLVPLQREMLSKIF-REDFIIWND 60
           MGGIGKTTIAQ VF C+LSKFDD CF TL  GDSKQSLV LQREMLS+IF +EDF IW++
Sbjct: 230 MGGIGKTTIAQVVFDCILSKFDDCCFLTLPGGDSKQSLVSLQREMLSQIFHKEDFRIWHE 289

Query: 61  IHGVEMIKNRLSSRKVLLVLVLDGVEEKMQLEKLVGSIDWLGPGSRVIVTTRNKELLYQP 120
            HGVEMIKNRLS RKVL  +VLDGVEE+ QLE L GS DW GPGSR+I+TTRNK LL  P
Sbjct: 290 NHGVEMIKNRLSGRKVL--IVLDGVEERRQLEMLAGSTDWFGPGSRIIITTRNKGLLCHP 349

Query: 121 NNVEVKEYNVEELDHDSAL 139
           N  E+KEYNVEELDHDSAL
Sbjct: 350 NYDEMKEYNVEELDHDSAL 366

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BLAST of Lag0039464 vs. NCBI nr
Match: KAA0039330.1 (TMV resistance protein N-like [Cucumis melo var. makuwa] >TYK00512.1 TMV resistance protein N-like [Cucumis melo var. makuwa])

HSP 1 Score: 204.5 bits (519), Expect = 6.0e-49
Identity = 106/139 (76.26%), Postives = 116/139 (83.45%), Query Frame = 0


Query: 1   MGGIGKTTIAQFVFYCLLSKFDDSCFPTLSRGDSKQSLVPLQREMLSKIF-REDFIIWND 60
           MGGIGKTTIAQ VF C+LSKFDD CF TL  GDSKQSLV LQREMLS+IF +EDF IW++
Sbjct: 229 MGGIGKTTIAQVVFDCILSKFDDCCFLTLPGGDSKQSLVSLQREMLSQIFHKEDFRIWHE 288

Query: 61  IHGVEMIKNRLSSRKVLLVLVLDGVEEKMQLEKLVGSIDWLGPGSRVIVTTRNKELLYQP 120
            HGVEMIKNRLS RKVL  +VLDGVEE+ QLE L GS DW GPGSR+I+TTRNK LL  P
Sbjct: 289 NHGVEMIKNRLSGRKVL--IVLDGVEERRQLEMLAGSTDWFGPGSRIIITTRNKGLLCHP 348

Query: 121 NNVEVKEYNVEELDHDSAL 139
           N  E+KEYNVEELDHDSAL
Sbjct: 349 NYDEMKEYNVEELDHDSAL 365

[Close]

BLAST of Lag0039464 vs. NCBI nr
Match: AAU04759.1 (MRGH11 [Cucumis melo])

HSP 1 Score: 201.4 bits (511), Expect = 5.0e-48
Identity = 104/139 (74.82%), Postives = 115/139 (82.73%), Query Frame = 0


Query: 1   MGGIGKTTIAQFVFYCLLSKFDDSCFPTLSRGDSKQSLVPLQREMLSKIF-REDFIIWND 60
           MGGIGKTTIAQ VF C+LSKFDD CF TL  GDSKQSLV LQREMLS+IF +EDF IW++
Sbjct: 229 MGGIGKTTIAQVVFDCILSKFDDCCFLTLPGGDSKQSLVSLQREMLSQIFHKEDFKIWHE 288

Query: 61  IHGVEMIKNRLSSRKVLLVLVLDGVEEKMQLEKLVGSIDWLGPGSRVIVTTRNKELLYQP 120
            HGVEMIKNRLS RKVL  +VLDG EE+ QLE L GS +W GPGSR+I+TTRNK LL  P
Sbjct: 289 NHGVEMIKNRLSGRKVL--IVLDGAEERRQLEMLAGSTEWFGPGSRIIITTRNKGLLCHP 348

Query: 121 NNVEVKEYNVEELDHDSAL 139
           N  E+KEYNVEELDHDSAL
Sbjct: 349 NYDEMKEYNVEELDHDSAL 365

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BLAST of Lag0039464 vs. NCBI nr
Match: XP_008459543.1 (PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_008459544.1 PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_008459545.1 PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_016902414.1 PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_016902415.1 PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_016902416.1 PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_016902417.1 PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 201.4 bits (511), Expect = 5.0e-48
Identity = 104/139 (74.82%), Postives = 115/139 (82.73%), Query Frame = 0


Query: 1   MGGIGKTTIAQFVFYCLLSKFDDSCFPTLSRGDSKQSLVPLQREMLSKIF-REDFIIWND 60
           MGGIGKTTIAQ VF C+LSKFDD CF TL  GDSKQSLV LQREMLS+IF +EDF IW++
Sbjct: 229 MGGIGKTTIAQVVFDCILSKFDDCCFLTLPGGDSKQSLVSLQREMLSQIFHKEDFKIWHE 288

Query: 61  IHGVEMIKNRLSSRKVLLVLVLDGVEEKMQLEKLVGSIDWLGPGSRVIVTTRNKELLYQP 120
            HGVEMIKNRLS RKVL  +VLDG EE+ QLE L GS +W GPGSR+I+TTRNK LL  P
Sbjct: 289 NHGVEMIKNRLSGRKVL--IVLDGAEERRQLEMLAGSTEWFGPGSRIIITTRNKGLLCHP 348

Query: 121 NNVEVKEYNVEELDHDSAL 139
           N  E+KEYNVEELDHDSAL
Sbjct: 349 NYDEMKEYNVEELDHDSAL 365

[Close]

BLAST of Lag0039464 vs. NCBI nr
Match: XP_011656060.2 (TMV resistance protein N isoform X1 [Cucumis sativus] >XP_011656061.2 TMV resistance protein N isoform X1 [Cucumis sativus] >XP_011656062.2 TMV resistance protein N isoform X1 [Cucumis sativus] >XP_031741444.1 TMV resistance protein N isoform X1 [Cucumis sativus] >KAE8648981.1 hypothetical protein Csa_009304 [Cucumis sativus])

HSP 1 Score: 200.3 bits (508), Expect = 1.1e-47
Identity = 103/139 (74.10%), Postives = 116/139 (83.45%), Query Frame = 0


Query: 1   MGGIGKTTIAQFVFYCLLSKFDDSCFPTLSRGDSKQSLVPLQREMLSKIF-REDFIIWND 60
           MGGIGKTTIAQ VF C+LSKF+D CF TL  GDSKQSLV LQREMLS+IF +EDF IW++
Sbjct: 229 MGGIGKTTIAQVVFDCILSKFEDCCFLTLPGGDSKQSLVSLQREMLSQIFHKEDFRIWHE 288

Query: 61  IHGVEMIKNRLSSRKVLLVLVLDGVEEKMQLEKLVGSIDWLGPGSRVIVTTRNKELLYQP 120
            HGVEMIKNRLS RKVL  +VLDG+EE+ QLE L GSI+W GPGSR+I+TTRNK LL  P
Sbjct: 289 NHGVEMIKNRLSGRKVL--IVLDGIEERRQLEMLAGSIEWFGPGSRIIITTRNKGLLCHP 348

Query: 121 NNVEVKEYNVEELDHDSAL 139
           N  E+K YNVEELDHDSAL
Sbjct: 349 NYDEMKVYNVEELDHDSAL 365

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BLAST of Lag0039464 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.3e-17
Identity = 55/141 (39.01%), Postives = 86/141 (60.99%), Query Frame = 0


Query: 1   MGGIGKTTIAQFVFYCLLSKFDDSCF-PTLSRGDSKQSLVPLQREMLSKIFREDFIIWND 60
           MGG+GKTT A+ +F      F+ +CF   +       +L+ LQ+ +LSK+ + +F+   D
Sbjct: 219 MGGVGKTTAARALFNRYYQNFESACFLEDVKEYLQHHTLLYLQKTLLSKLLKVEFVDCTD 278

Query: 61  IHGV-EMIKNRLSSRKVLLVLVLDGVEEKMQLEKLVGSIDWLGPGSRVIVTTRNKELLYQ 120
              +  ++K RL S+KVL  +VLD V    QL+KLVG+ DW G GSR+++TTR+ +LL  
Sbjct: 279 TEEMCVILKRRLCSKKVL--VVLDDVNHNDQLDKLVGAEDWFGSGSRIVITTRDMKLL-- 338

Query: 121 PNNVEVKE-YNVEELDHDSAL 139
             N +V E Y ++ L+ D A+
Sbjct: 339 -KNHDVHETYEIKVLEKDEAI 354

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BLAST of Lag0039464 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.1e-16
Identity = 57/144 (39.58%), Postives = 85/144 (59.03%), Query Frame = 0


Query: 1   MGGIGKTTIAQFVFYCLLSK------FDDSCFPTLSRGDSKQSLVPLQREMLSKIFREDF 60
           MGG+GKTTIA+ +F  LL +      FD +CF      ++K+ +  LQ  +LS++ RE  
Sbjct: 217 MGGVGKTTIARAIFDTLLGRMDSSYQFDGACF-LKDIKENKRGMHSLQNALLSELLREKA 276

Query: 61  IIWNDIHGVEMIKNRLSSRKVLLVLVLDGVEEK-MQLEKLVGSIDWLGPGSRVIVTTRNK 120
              N+  G   + +RL S+KVL  +VLD ++ K   LE L G +DW G GSR+I+TTR+K
Sbjct: 277 NYNNEEDGKHQMASRLRSKKVL--IVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITTRDK 336

Query: 121 ELLYQPNNVEVKEYNVEEL-DHDS 137
            L+ + + +    Y V  L DH+S
Sbjct: 337 HLIEKNDII----YEVTALPDHES 353

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BLAST of Lag0039464 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 4.5e-15
Identity = 58/141 (41.13%), Postives = 82/141 (58.16%), Query Frame = 0


Query: 1   MGGIGKTTIAQFVFYCLLSKFDDSCF-PTLSRGDSKQSLVPLQREMLSKIFREDFI--IW 60
           +GGIGKTTIA+ ++  L  +F+   F   +  G + Q L  LQ ++L  I   +    I 
Sbjct: 225 VGGIGKTTIAKVIYNELSCEFEYMSFLENIREGSNPQVLFHLQNQLLGDILEGEGSQNIS 284

Query: 61  NDIHGVEMIKNRLSSRKVLLVLVLDGVEEKMQLEKLVGSIDWLGPGSRVIVTTRNKELLY 120
           +  H   MIK+ L SR+V   +VLD V++  QLE L+G  +WLG GSRVI+TTRNK +L 
Sbjct: 285 SVAHRASMIKDILLSRRVF--IVLDDVDDLSQLEYLLGHREWLGEGSRVIITTRNKHVLA 344

Query: 121 QPNNVEVKE-YNVEELDHDSA 138
                EV + Y VE L+ + A
Sbjct: 345 VQ---EVDDLYEVEGLNFEEA 360

[Close]

BLAST of Lag0039464 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 5.8e-15
Identity = 55/137 (40.15%), Postives = 82/137 (59.85%), Query Frame = 0


Query: 1   MGGIGKTTIAQFVFYCLLSKFDDSCF-PTLSRGDSKQSLVPLQREMLSKIFREDFI--IW 60
           +GGIGKTTIA+ ++  L  +F+   F   +    + Q + PLQ ++L  I + +    I 
Sbjct: 230 VGGIGKTTIAKVIYNELSREFEYMSFLENIREKFNTQGVSPLQNQLLDDILKGEGSQNIN 289

Query: 61  NDIHGVEMIKNRLSSRKVLLVLVLDGVEEKMQLEKLVGSIDWLGPGSRVIVTTRNKELL- 120
           +  HG  MIK+ LSS+  ++ +VLD V+++ QLE L+   +WLG GSRVI+TTRNK +L 
Sbjct: 290 SVAHGASMIKDILSSK--IVFIVLDDVDDQSQLEYLLRHREWLGEGSRVIITTRNKHVLD 349

Query: 121 --YQPNNVEVKEYNVEE 132
                +  EVK  N EE
Sbjct: 350 VQKVDDLYEVKGLNFEE 364

[Close]

BLAST of Lag0039464 vs. ExPASy Swiss-Prot
Match: Q9CAK1 (Disease resistance protein RML1B OS=Arabidopsis thaliana OX=3702 GN=RLM1B PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 3.8e-14
Identity = 50/122 (40.98%), Postives = 67/122 (54.92%), Query Frame = 0


Query: 3   GIGKTTIAQFVFYCLLSKFDDSCFPTLSRG------DSKQSLVPLQREMLSKIFREDFII 62
           GIGKTTIA+ ++  L  +F  SCF    RG      D     + LQ + LSK+  +  + 
Sbjct: 218 GIGKTTIARALYGLLSKRFQLSCFVDNLRGSYHSGFDEYGFKLHLQEQFLSKVLNQSGM- 277

Query: 63  WNDIHGVEMIKNRLSSRKVLLVLVLDGVEEKMQLEKLVGSIDWLGPGSRVIVTTRNKELL 119
              I  +  IK  LS ++VL  ++LD V +  QLE L     W GPGSR++VTT NKELL
Sbjct: 278 --RICHLGAIKENLSDQRVL--IILDDVNKLKQLEALANETTWFGPGSRIVVTTENKELL 334

The following BLAST results are available for this feature:

BLAST of Lag0039464 vs. NCBI nr


Match Name	E-value	Identity	Description
AGH33850.1	6.0e-49	76.26	resistance gene-like protein [Cucumis melo]	[more]
KAA0039330.1	6.0e-49	76.26	TMV resistance protein N-like [Cucumis melo var. makuwa] >TYK00512.1 TMV resista...	[more]
AAU04759.1	5.0e-48	74.82	MRGH11 [Cucumis melo]	[more]
XP_008459543.1	5.0e-48	74.82	PREDICTED: TMV resistance protein N-like [Cucumis melo] >XP_008459544.1 PREDICTE...	[more]
XP_011656060.2	1.1e-47	74.10	TMV resistance protein N isoform X1 [Cucumis sativus] >XP_011656061.2 TMV resist...	[more]

BLAST of Lag0039464 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A290U7C4	1.3e-17	39.01	Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV...	[more]
Q40392	3.1e-16	39.58	TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1	[more]
V9M2S5	4.5e-15	41.13	Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=...	[more]
V9M398	5.8e-15	40.15	Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=...	[more]
Q9CAK1	3.8e-14	40.98	Disease resistance protein RML1B OS=Arabidopsis thaliana OX=3702 GN=RLM1B PE=2 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 1..126 e-value: 6.4E-20 score: 73.4
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 1..138
IPR002182	NB-ARC	PFAM	PF00931	NB-ARC	coord: 1..137 e-value: 8.9E-12 score: 44.7
IPR044974	Disease resistance protein, plants	PANTHER	PTHR11017	LEUCINE-RICH REPEAT-CONTAINING PROTEIN	coord: 1..138
None	No IPR available	PANTHER	PTHR11017:SF219	ARCHAEAL ATPASE	coord: 1..138

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0039464.1	Lag0039464.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0007165	signal transduction
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0003953	NAD+ nucleosidase activity