Lag0039426 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0039426 vs. NCBI nr
Match: XP_022141775.1 (TMV resistance protein N-like [Momordica charantia])

HSP 1 Score: 233.4 bits (594), Expect = 4.5e-57
Identity = 172/536 (32.09%), Postives = 270/536 (50.37%), Query Frame = 0

Query: 15   FMIRLETDGYPPKCLRNVEKECGMFSRAKVCVFYIPFEKLAEEVRNEDFRYIKASITSKN 74
            F+I L  + +P   +  +  +  + SR +  + +IP   L +       R+   +  S N
Sbjct: 1071 FLIELGMEEHPRTGILPLTSK--LTSRTRFWMLFIPLNLLPK----SRLRFKILAKGSPN 1130

Query: 75   PDIKVEKCGASVLFREDVVVFLRNMLNKCF--QNRPIFTRISKDRSHLSEGKYVEVEDPD 134
              I ++KCGASVL  ++    L NM NK +  +N+  F    ++ +H S+    E E  D
Sbjct: 1131 -TINIQKCGASVLSHQNAPELLANMFNKVYRTRNQDEFCTTIRECNHGSKCSCDEPETID 1190

Query: 135  QDDFSSTNEDSSSEHSIILLKRSLKSIIRRTFEEENVRLAPLYMFPRKEMSISWFKHQQE 194
            Q  + ST    +      LLKRSLKSI+RRT+EEE    +  Y FPR   ++SWF+ QQ 
Sbjct: 1191 Q--YCSTQYVENESGPFSLLKRSLKSILRRTYEEEKDH-SLYYFFPRGP-ALSWFRIQQP 1250

Query: 195  QATLTIKLPLCLHKDKKWMGLALFVVFSVEGH--LGRHHTFSYNIDGDE--------VQY 254
            + T+ +KLPL L K+KKWMGLA+F +FSV G+     +HTF++ I  +E          Y
Sbjct: 1251 KDTVAVKLPLDLFKEKKWMGLAVFAIFSVPGNSEYVHNHTFNFQITSNEDGRHLIKGGSY 1310

Query: 255  AHSPSIKMPKYLVDIDSSQQVCVLFNSRASFSYALNCSDHIHANITTEP-GVKVVSCGAR 314
            + S S  +P  L+   S Q + V F  R  F Y     +H + +  + P  ++V SCGAR
Sbjct: 1311 S-SGSFLIPLSLLQ-SSPQILFVTFEPRHVFPYVQEGLNHFYVSFISIPTRLQVESCGAR 1370

Query: 315  LVYQQNVRGLIETIVDCVCQSPPYLHDYQTTMILASFLTS-------------ITALGDT 374
            LVYQ NV GLI TI+DCV +S   L+   +  I+  FLT+             +T    +
Sbjct: 1371 LVYQHNVEGLINTIMDCVPKSREELYQDHSQEIMRDFLTTYHRPIRIQVPADQLTTSTSS 1430

Query: 375  PPPGVQKQDVIELIM---KDPFR-TGFDCFLYGHECLSLLTFQK-GNNAKMKLPQGLCLN 434
               G ++     L M   +D +  +  +  ++G E     T Q  GN   ++ P+ L   
Sbjct: 1431 DLSGRRESFQHSLFMESTQDQYNISKLEAIIHGKEIPDFFTSQSMGNKGAVRTPERLYFA 1490

Query: 435  GSWMGFVTYALLTLNRPTTNDHEFKQPLKIVDLVCQFHADSDLIEPDHFLITSEDDLLEG 494
               +G +  A++ +N    N  E  + +K++DL C+F  DS  IEP+H L    + LL  
Sbjct: 1491 KCEVGVLVCAVVVIN----NQTEKLEHVKVLDLKCEFGVDSHEIEPEHHLFVPAEKLLSS 1550

Query: 495  GADQFIWLFYVPRTELDIVWHHSTYFQASMVTNRPELLSVRECGIQIVSLQDKEII 520
                FIWL  +P TE +I W    Y +  + TN  EL  V++CG+ ++   +++++
Sbjct: 1551 FESSFIWLSCIPLTEFNIPWMCCDYLRCKVSTNHEELFHVQQCGLHLLYASERKMV 1589

BLAST of Lag0039426 vs. NCBI nr
Match: XP_038890618.1 (TMV resistance protein N-like [Benincasa hispida])

HSP 1 Score: 232.3 bits (591), Expect = 1.0e-56
Identity = 172/596 (28.86%), Postives = 282/596 (47.32%), Query Frame = 0

Query: 15   FMIRLETDGYPPKCLRNVE-KECGMFSRAKVCVFYIPFEKLAEEVRNEDFRYIKASITSK 74
            F++++ETD  P K     +  +  + S+  + VFYIP ++++  +   +   I  SI + 
Sbjct: 1080 FIVKMETDECPLKSALVFDGNKAELESQMGLSVFYIPMKRISRWL--NECCCIDVSIMTD 1139

Query: 75   NPDIKVEKCGASVLFREDVVVFLRNMLNKCF-----------------QNRPIFTRI--- 134
            NP +KV+ CGAS+L+ ++   F+  ++   F                 QNR   + +   
Sbjct: 1140 NPFVKVKWCGASILYEQNAGSFIGKIIKALFGSPGKYHTSIVDHLLNRQNRVDVSTLLDG 1199

Query: 135  -----------------SKDRSHLSEGKYVEVEDPDQDDFSSTNEDSSSEHSIILLKRSL 194
                             S  R   S      +E+    + SS  E++ S+HS I+LKR+L
Sbjct: 1200 GARYKTSWFNALQRTIGSFSRLRPSRPPREVIEECSTMNASSEAEENESDHS-IMLKRNL 1259

Query: 195  KSIIRRTFEEENVRLAPLYMFPRKEMSISWFKHQQEQATLTIKLPLCLHKDKKWMGLALF 254
            K+ + RTFEE  +     YMFP+KEMS S+F  Q ++  +TIK+P  LHK+KKWMGLA F
Sbjct: 1260 KATLLRTFEELKL-YGEYYMFPQKEMSRSFFNFQLKEPKITIKVPPNLHKEKKWMGLAFF 1319

Query: 255  VVFSVEGHLGRHHTFSYNIDGDEVQYAHSPSIKMPKYLVDIDSSQQVCVLFNSRASFSYA 314
            VVFSV+    + H+FSY++D DE +      + + + L  +  S Q+ + F  RA + Y 
Sbjct: 1320 VVFSVDESSPKAHSFSYHVDNDEYRLERESILYLNEDL--LVDSHQLWLFFEPRAVYPYR 1379

Query: 315  LNCSDHIHANIT-TEPGVKVVSCGARLVYQQNVRGLIETIVDCVCQSPPYLHDYQTTMIL 374
            LN   H+  +I       KVV CGARLVY+Q++ G + TIV  V  SP  LH+Y   + +
Sbjct: 1380 LNQWRHLRFSIVCNNSDFKVVLCGARLVYKQDLEGFVNTIVSNVLNSPTELHEYYDQIHV 1439

Query: 375  ASFLTSITALGDTPP---------------PGVQKQDV---------------------- 434
               L ++ +    P                 G QK +                       
Sbjct: 1440 NGMLKNVHSHKYDPKKKENESRQDFPIEEWEGEQKSNAHPQEEDCSKMERSHILQLKESI 1499

Query: 435  ---IELIMKDPFRTGFDCFLYGHECLSLLTFQKG--NNAKMKLPQGLCLNGSWMGFVTYA 494
               ++  +KD F T FD  +        L  Q    +   ++LP  L  N  WMGF  + 
Sbjct: 1500 PSFLQKDLKDRFETPFDFVIPRRNVRPQLINQLSPKSYTSIQLPPNLYTNTDWMGFAVWT 1559

Query: 495  LLTLNRPTTNDHEFKQPLKIVDLVCQFHADSDLIEPDHFLITSEDDLLEGGADQFIWLFY 530
            L  +N+  T        +   +L+CQF  ++ LI P H     ED ++     QF+WL+Y
Sbjct: 1560 LFQINKHPTAILNNVGSVSRHELICQFGIENGLINPLHIHTIIEDTVIWLHERQFVWLYY 1619

BLAST of Lag0039426 vs. NCBI nr
Match: XP_022141874.1 (TMV resistance protein N-like isoform X1 [Momordica charantia])

HSP 1 Score: 232.3 bits (591), Expect = 1.0e-56
Identity = 174/597 (29.15%), Postives = 277/597 (46.40%), Query Frame = 0

Query: 15   FMIRLETDGYPPKCLRNVEKECG-MFSRAKVCVFYIPFEKLAEEVRNEDFRYIKASITSK 74
            F+IR+ETD  P K    ++     + S   + VFYIP  K++E +       I  SI + 
Sbjct: 1071 FIIRMETDECPLKSALVIDGNTDELDSPHGLVVFYIPMTKISEWL--NQCCCIDVSIITD 1130

Query: 75   NPDIKVEKCGASVLFREDVVVFLRNMLNKCFQNRP----------IFTRISK-DRSHLSE 134
            NP +KV+ CGAS+L+ ++   F+  ++ K F   P          I  R  + D S L +
Sbjct: 1131 NPLVKVKWCGASILYEQNAGKFIGRII-KSFFGSPGKYHTSIVDHILNRQKRVDVSSLLD 1190

Query: 135  G--KYVE-------------------------VEDPDQDDFSSTNEDSSSEHSIILLKRS 194
            G  +Y                           +ED      +S     +   SII+LKR+
Sbjct: 1191 GGARYKTCWLNALQRTIGSFPRLRPSRPPPEVIEDCSTSTNASVEAQENESDSIIMLKRN 1250

Query: 195  LKSIIRRTFEEENVRLAPLYMFPRKEMSISWFKHQQEQATLTIKLPLCLHKDKKWMGLAL 254
            LK+++ RTFEE  +     ++FP+KE+S SWF  Q ++  +TIK+P  LHKDKKWMGLA 
Sbjct: 1251 LKAVLLRTFEELKL-YGEYFVFPQKEISRSWFTLQLKKPKVTIKVPPNLHKDKKWMGLAF 1310

Query: 255  FVVFSVEGHLGRHHTFSYNIDGDEVQYAHSPSIKMPKYLVDIDSSQQVCVLFNSRASFSY 314
            FVVF+V+    + H+FSY ++ DE        + + K +   +   Q+ + +  RA + Y
Sbjct: 1311 FVVFAVDEKSTKSHSFSYQVENDEYTMQRESILYLNKEM--FNDYHQLWLFYEPRAVYPY 1370

Query: 315  ALNCSDHIHAN-ITTEPGVKVVSCGARLVYQQNVRGLIETIVDCVCQSPPYLHDYQTTMI 374
             LN   H+  + ++  P +K V+CGARLVY+Q++ G I+ I++ V   PP LH +   + 
Sbjct: 1371 RLNHWRHLCVSFLSNNPDLKAVACGARLVYKQDLEGFIQMIINNVLSCPPDLHGFYDQVY 1430

Query: 375  LASFLTSITALGDTPPP---------------------GVQKQD---------------- 434
            + + L  I      P                       G   QD                
Sbjct: 1431 VEAMLRMIHFHKYDPKEKEEQRRQDLCLEQWEAEQNLNGHSDQDYSAQNLGGNHILQLKE 1490

Query: 435  ----VIELIMKDPFRTGFDCFLYGHECLSLLTFQKGNN-AKMKLPQGLCLNGSWMGFVTY 494
                 ++  +KD F T FD  +       L   Q   N   ++LP  L  N +W+GF   
Sbjct: 1491 SIPSFLQKDLKDRFGTTFDFVIPRRHIPQLFNQQSTKNYTAIELPPSLYTNSNWIGFAVC 1550

Query: 495  ALLTLNRPTTNDHEFKQPLKIVDLVCQFHADSDLIEPDHFLITSEDDLLEGGADQFIWLF 530
             L  +N+  T      +     +L+CQF  ++ LIEP H    +ED  +     QF+WL+
Sbjct: 1551 TLFQVNKHPTAILNNLRSASRHELICQFAVENGLIEPFHIHTITEDTFIWLHERQFVWLY 1610

BLAST of Lag0039426 vs. NCBI nr
Match: XP_022141875.1 (uncharacterized protein LOC111012131 isoform X2 [Momordica charantia])

HSP 1 Score: 232.3 bits (591), Expect = 1.0e-56
Identity = 174/597 (29.15%), Postives = 277/597 (46.40%), Query Frame = 0

Query: 15   FMIRLETDGYPPKCLRNVEKECG-MFSRAKVCVFYIPFEKLAEEVRNEDFRYIKASITSK 74
            F+IR+ETD  P K    ++     + S   + VFYIP  K++E +       I  SI + 
Sbjct: 861  FIIRMETDECPLKSALVIDGNTDELDSPHGLVVFYIPMTKISEWL--NQCCCIDVSIITD 920

Query: 75   NPDIKVEKCGASVLFREDVVVFLRNMLNKCFQNRP----------IFTRISK-DRSHLSE 134
            NP +KV+ CGAS+L+ ++   F+  ++ K F   P          I  R  + D S L +
Sbjct: 921  NPLVKVKWCGASILYEQNAGKFIGRII-KSFFGSPGKYHTSIVDHILNRQKRVDVSSLLD 980

Query: 135  G--KYVE-------------------------VEDPDQDDFSSTNEDSSSEHSIILLKRS 194
            G  +Y                           +ED      +S     +   SII+LKR+
Sbjct: 981  GGARYKTCWLNALQRTIGSFPRLRPSRPPPEVIEDCSTSTNASVEAQENESDSIIMLKRN 1040

Query: 195  LKSIIRRTFEEENVRLAPLYMFPRKEMSISWFKHQQEQATLTIKLPLCLHKDKKWMGLAL 254
            LK+++ RTFEE  +     ++FP+KE+S SWF  Q ++  +TIK+P  LHKDKKWMGLA 
Sbjct: 1041 LKAVLLRTFEELKL-YGEYFVFPQKEISRSWFTLQLKKPKVTIKVPPNLHKDKKWMGLAF 1100

Query: 255  FVVFSVEGHLGRHHTFSYNIDGDEVQYAHSPSIKMPKYLVDIDSSQQVCVLFNSRASFSY 314
            FVVF+V+    + H+FSY ++ DE        + + K +   +   Q+ + +  RA + Y
Sbjct: 1101 FVVFAVDEKSTKSHSFSYQVENDEYTMQRESILYLNKEM--FNDYHQLWLFYEPRAVYPY 1160

Query: 315  ALNCSDHIHAN-ITTEPGVKVVSCGARLVYQQNVRGLIETIVDCVCQSPPYLHDYQTTMI 374
             LN   H+  + ++  P +K V+CGARLVY+Q++ G I+ I++ V   PP LH +   + 
Sbjct: 1161 RLNHWRHLCVSFLSNNPDLKAVACGARLVYKQDLEGFIQMIINNVLSCPPDLHGFYDQVY 1220

Query: 375  LASFLTSITALGDTPPP---------------------GVQKQD---------------- 434
            + + L  I      P                       G   QD                
Sbjct: 1221 VEAMLRMIHFHKYDPKEKEEQRRQDLCLEQWEAEQNLNGHSDQDYSAQNLGGNHILQLKE 1280

Query: 435  ----VIELIMKDPFRTGFDCFLYGHECLSLLTFQKGNN-AKMKLPQGLCLNGSWMGFVTY 494
                 ++  +KD F T FD  +       L   Q   N   ++LP  L  N +W+GF   
Sbjct: 1281 SIPSFLQKDLKDRFGTTFDFVIPRRHIPQLFNQQSTKNYTAIELPPSLYTNSNWIGFAVC 1340

Query: 495  ALLTLNRPTTNDHEFKQPLKIVDLVCQFHADSDLIEPDHFLITSEDDLLEGGADQFIWLF 530
             L  +N+  T      +     +L+CQF  ++ LIEP H    +ED  +     QF+WL+
Sbjct: 1341 TLFQVNKHPTAILNNLRSASRHELICQFAVENGLIEPFHIHTITEDTFIWLHERQFVWLY 1400

BLAST of Lag0039426 vs. NCBI nr
Match: XP_038889439.1 (TMV resistance protein N-like [Benincasa hispida])

HSP 1 Score: 229.9 bits (585), Expect = 5.0e-56
Identity = 169/596 (28.36%), Postives = 272/596 (45.64%), Query Frame = 0

Query: 15   FMIRLETDGYPPKCLRNVE-KECGMFSRAKVCVFYIPFEKLAEEVRNEDFRYIKASITSK 74
            F++++ETD  P K     +  +  + S   + VFYIP  +++  +       I  SI + 
Sbjct: 1081 FIVKMETDECPLKSALVFDGNKDELESPVGLSVFYIPMRRISGWL--NQCCCIDVSIMTD 1140

Query: 75   NPDIKVEKCGASVLFREDVVVFLRNMLNKCF-----------------QNRPIFTRISKD 134
            NP +KV+ CGAS+L+ ++   F+  ++   F                 QNR   + +   
Sbjct: 1141 NPLVKVKWCGASILYEQNAGSFIGKIIKAFFGSPGRYHTSIVDHILNRQNRVDVSTLLDG 1200

Query: 135  RSHL--------------------SEGKYVEVEDPDQDDFSSTNEDSSSEHSIILLKRSL 194
             +H                     S      +ED    + SS  +++ S++S I+LKR++
Sbjct: 1201 GAHYKTTWFNALQRTIGSFPRLRPSRPPREVIEDCSTMNASSEIDENESDYS-IMLKRNI 1260

Query: 195  KSIIRRTFEEENVRLAPLYMFPRKEMSISWFKHQQEQATLTIKLPLCLHKDKKWMGLALF 254
            K+ + RTFEE  +     Y+FP+KE+S SWF  Q ++  +TIK+   LHKDKKWMGLA F
Sbjct: 1261 KATLERTFEELKL-YGEYYIFPQKEISRSWFNFQLKEPKITIKISPNLHKDKKWMGLAFF 1320

Query: 255  VVFSVEGHLGRHHTFSYNIDGDEVQYAHSPSIKMPKYLVDIDSSQQVCVLFNSRASFSYA 314
            VVFS + +  + H+FSY ++ DE        I + + L   D S Q+ + F  RA + Y 
Sbjct: 1321 VVFSADENSPKSHSFSYQVENDEYTMQRQSIIYLNEEL--FDDSHQLWMFFEPRAVYPYR 1380

Query: 315  LNCSDHIH-ANITTEPGVKVVSCGARLVYQQNVRGLIETIVDCVCQSPPYLHDYQTTMIL 374
            LN   H+  A +  +   K V CGARLVY+Q+V G + TIV  V  SP  LH++     +
Sbjct: 1381 LNQWRHLRFAFVCNDSDFKAVLCGARLVYKQDVEGFVNTIVSNVLSSPVELHEFYDQSYV 1440

Query: 375  ASFLTSI--------------------------------TALGDTPPPGVQKQDVIEL-- 434
               L ++                                     T  P +++  +++L  
Sbjct: 1441 KGMLRNVQFHKYDPKNKEEETRQDLLIQEWEEEQNSNAYPQQDSTSSPNMERSHILQLKE 1500

Query: 435  --------IMKDPFRTGFDCFLYGHECLSLLT-FQKGNNAKMKLPQGLCLNGSWMGFVTY 494
                      KD F+  FD  +       L       N+  ++LP  L     WMGF+  
Sbjct: 1501 SIPSFLQKDSKDRFQNTFDFVIPRRNFPQLFNQLSPKNHTGIELPPNLYTTNDWMGFLVC 1560

Query: 495  ALLTLNRPTTNDHEFKQPLKIVDLVCQFHADSDLIEPDHFLITSEDDLLEGGADQFIWLF 529
             L  +N+  T        +   +L+CQF  ++ LIEP H    +ED  +     QF+WL+
Sbjct: 1561 TLFQVNKHPTAILNNLGSITRHELICQFAIENGLIEPLHLHSITEDRFIWLHERQFVWLY 1620

BLAST of Lag0039426 vs. ExPASy TrEMBL
Match: A0A6J1CJ31 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012055 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.2e-57
Identity = 172/536 (32.09%), Postives = 270/536 (50.37%), Query Frame = 0

Query: 15   FMIRLETDGYPPKCLRNVEKECGMFSRAKVCVFYIPFEKLAEEVRNEDFRYIKASITSKN 74
            F+I L  + +P   +  +  +  + SR +  + +IP   L +       R+   +  S N
Sbjct: 1071 FLIELGMEEHPRTGILPLTSK--LTSRTRFWMLFIPLNLLPK----SRLRFKILAKGSPN 1130

Query: 75   PDIKVEKCGASVLFREDVVVFLRNMLNKCF--QNRPIFTRISKDRSHLSEGKYVEVEDPD 134
              I ++KCGASVL  ++    L NM NK +  +N+  F    ++ +H S+    E E  D
Sbjct: 1131 -TINIQKCGASVLSHQNAPELLANMFNKVYRTRNQDEFCTTIRECNHGSKCSCDEPETID 1190

Query: 135  QDDFSSTNEDSSSEHSIILLKRSLKSIIRRTFEEENVRLAPLYMFPRKEMSISWFKHQQE 194
            Q  + ST    +      LLKRSLKSI+RRT+EEE    +  Y FPR   ++SWF+ QQ 
Sbjct: 1191 Q--YCSTQYVENESGPFSLLKRSLKSILRRTYEEEKDH-SLYYFFPRGP-ALSWFRIQQP 1250

Query: 195  QATLTIKLPLCLHKDKKWMGLALFVVFSVEGH--LGRHHTFSYNIDGDE--------VQY 254
            + T+ +KLPL L K+KKWMGLA+F +FSV G+     +HTF++ I  +E          Y
Sbjct: 1251 KDTVAVKLPLDLFKEKKWMGLAVFAIFSVPGNSEYVHNHTFNFQITSNEDGRHLIKGGSY 1310

Query: 255  AHSPSIKMPKYLVDIDSSQQVCVLFNSRASFSYALNCSDHIHANITTEP-GVKVVSCGAR 314
            + S S  +P  L+   S Q + V F  R  F Y     +H + +  + P  ++V SCGAR
Sbjct: 1311 S-SGSFLIPLSLLQ-SSPQILFVTFEPRHVFPYVQEGLNHFYVSFISIPTRLQVESCGAR 1370

Query: 315  LVYQQNVRGLIETIVDCVCQSPPYLHDYQTTMILASFLTS-------------ITALGDT 374
            LVYQ NV GLI TI+DCV +S   L+   +  I+  FLT+             +T    +
Sbjct: 1371 LVYQHNVEGLINTIMDCVPKSREELYQDHSQEIMRDFLTTYHRPIRIQVPADQLTTSTSS 1430

Query: 375  PPPGVQKQDVIELIM---KDPFR-TGFDCFLYGHECLSLLTFQK-GNNAKMKLPQGLCLN 434
               G ++     L M   +D +  +  +  ++G E     T Q  GN   ++ P+ L   
Sbjct: 1431 DLSGRRESFQHSLFMESTQDQYNISKLEAIIHGKEIPDFFTSQSMGNKGAVRTPERLYFA 1490

Query: 435  GSWMGFVTYALLTLNRPTTNDHEFKQPLKIVDLVCQFHADSDLIEPDHFLITSEDDLLEG 494
               +G +  A++ +N    N  E  + +K++DL C+F  DS  IEP+H L    + LL  
Sbjct: 1491 KCEVGVLVCAVVVIN----NQTEKLEHVKVLDLKCEFGVDSHEIEPEHHLFVPAEKLLSS 1550

Query: 495  GADQFIWLFYVPRTELDIVWHHSTYFQASMVTNRPELLSVRECGIQIVSLQDKEII 520
                FIWL  +P TE +I W    Y +  + TN  EL  V++CG+ ++   +++++
Sbjct: 1551 FESSFIWLSCIPLTEFNIPWMCCDYLRCKVSTNHEELFHVQQCGLHLLYASERKMV 1589

BLAST of Lag0039426 vs. ExPASy TrEMBL
Match: A0A6J1CK08 (TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012131 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 4.9e-57
Identity = 174/597 (29.15%), Postives = 277/597 (46.40%), Query Frame = 0

Query: 15   FMIRLETDGYPPKCLRNVEKECG-MFSRAKVCVFYIPFEKLAEEVRNEDFRYIKASITSK 74
            F+IR+ETD  P K    ++     + S   + VFYIP  K++E +       I  SI + 
Sbjct: 1071 FIIRMETDECPLKSALVIDGNTDELDSPHGLVVFYIPMTKISEWL--NQCCCIDVSIITD 1130

Query: 75   NPDIKVEKCGASVLFREDVVVFLRNMLNKCFQNRP----------IFTRISK-DRSHLSE 134
            NP +KV+ CGAS+L+ ++   F+  ++ K F   P          I  R  + D S L +
Sbjct: 1131 NPLVKVKWCGASILYEQNAGKFIGRII-KSFFGSPGKYHTSIVDHILNRQKRVDVSSLLD 1190

Query: 135  G--KYVE-------------------------VEDPDQDDFSSTNEDSSSEHSIILLKRS 194
            G  +Y                           +ED      +S     +   SII+LKR+
Sbjct: 1191 GGARYKTCWLNALQRTIGSFPRLRPSRPPPEVIEDCSTSTNASVEAQENESDSIIMLKRN 1250

Query: 195  LKSIIRRTFEEENVRLAPLYMFPRKEMSISWFKHQQEQATLTIKLPLCLHKDKKWMGLAL 254
            LK+++ RTFEE  +     ++FP+KE+S SWF  Q ++  +TIK+P  LHKDKKWMGLA 
Sbjct: 1251 LKAVLLRTFEELKL-YGEYFVFPQKEISRSWFTLQLKKPKVTIKVPPNLHKDKKWMGLAF 1310

Query: 255  FVVFSVEGHLGRHHTFSYNIDGDEVQYAHSPSIKMPKYLVDIDSSQQVCVLFNSRASFSY 314
            FVVF+V+    + H+FSY ++ DE        + + K +   +   Q+ + +  RA + Y
Sbjct: 1311 FVVFAVDEKSTKSHSFSYQVENDEYTMQRESILYLNKEM--FNDYHQLWLFYEPRAVYPY 1370

Query: 315  ALNCSDHIHAN-ITTEPGVKVVSCGARLVYQQNVRGLIETIVDCVCQSPPYLHDYQTTMI 374
             LN   H+  + ++  P +K V+CGARLVY+Q++ G I+ I++ V   PP LH +   + 
Sbjct: 1371 RLNHWRHLCVSFLSNNPDLKAVACGARLVYKQDLEGFIQMIINNVLSCPPDLHGFYDQVY 1430

Query: 375  LASFLTSITALGDTPPP---------------------GVQKQD---------------- 434
            + + L  I      P                       G   QD                
Sbjct: 1431 VEAMLRMIHFHKYDPKEKEEQRRQDLCLEQWEAEQNLNGHSDQDYSAQNLGGNHILQLKE 1490

Query: 435  ----VIELIMKDPFRTGFDCFLYGHECLSLLTFQKGNN-AKMKLPQGLCLNGSWMGFVTY 494
                 ++  +KD F T FD  +       L   Q   N   ++LP  L  N +W+GF   
Sbjct: 1491 SIPSFLQKDLKDRFGTTFDFVIPRRHIPQLFNQQSTKNYTAIELPPSLYTNSNWIGFAVC 1550

Query: 495  ALLTLNRPTTNDHEFKQPLKIVDLVCQFHADSDLIEPDHFLITSEDDLLEGGADQFIWLF 530
             L  +N+  T      +     +L+CQF  ++ LIEP H    +ED  +     QF+WL+
Sbjct: 1551 TLFQVNKHPTAILNNLRSASRHELICQFAVENGLIEPFHIHTITEDTFIWLHERQFVWLY 1610

BLAST of Lag0039426 vs. ExPASy TrEMBL
Match: A0A6J1CJB7 (uncharacterized protein LOC111012131 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012131 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 4.9e-57
Identity = 174/597 (29.15%), Postives = 277/597 (46.40%), Query Frame = 0

Query: 15   FMIRLETDGYPPKCLRNVEKECG-MFSRAKVCVFYIPFEKLAEEVRNEDFRYIKASITSK 74
            F+IR+ETD  P K    ++     + S   + VFYIP  K++E +       I  SI + 
Sbjct: 861  FIIRMETDECPLKSALVIDGNTDELDSPHGLVVFYIPMTKISEWL--NQCCCIDVSIITD 920

Query: 75   NPDIKVEKCGASVLFREDVVVFLRNMLNKCFQNRP----------IFTRISK-DRSHLSE 134
            NP +KV+ CGAS+L+ ++   F+  ++ K F   P          I  R  + D S L +
Sbjct: 921  NPLVKVKWCGASILYEQNAGKFIGRII-KSFFGSPGKYHTSIVDHILNRQKRVDVSSLLD 980

Query: 135  G--KYVE-------------------------VEDPDQDDFSSTNEDSSSEHSIILLKRS 194
            G  +Y                           +ED      +S     +   SII+LKR+
Sbjct: 981  GGARYKTCWLNALQRTIGSFPRLRPSRPPPEVIEDCSTSTNASVEAQENESDSIIMLKRN 1040

Query: 195  LKSIIRRTFEEENVRLAPLYMFPRKEMSISWFKHQQEQATLTIKLPLCLHKDKKWMGLAL 254
            LK+++ RTFEE  +     ++FP+KE+S SWF  Q ++  +TIK+P  LHKDKKWMGLA 
Sbjct: 1041 LKAVLLRTFEELKL-YGEYFVFPQKEISRSWFTLQLKKPKVTIKVPPNLHKDKKWMGLAF 1100

Query: 255  FVVFSVEGHLGRHHTFSYNIDGDEVQYAHSPSIKMPKYLVDIDSSQQVCVLFNSRASFSY 314
            FVVF+V+    + H+FSY ++ DE        + + K +   +   Q+ + +  RA + Y
Sbjct: 1101 FVVFAVDEKSTKSHSFSYQVENDEYTMQRESILYLNKEM--FNDYHQLWLFYEPRAVYPY 1160

Query: 315  ALNCSDHIHAN-ITTEPGVKVVSCGARLVYQQNVRGLIETIVDCVCQSPPYLHDYQTTMI 374
             LN   H+  + ++  P +K V+CGARLVY+Q++ G I+ I++ V   PP LH +   + 
Sbjct: 1161 RLNHWRHLCVSFLSNNPDLKAVACGARLVYKQDLEGFIQMIINNVLSCPPDLHGFYDQVY 1220

Query: 375  LASFLTSITALGDTPPP---------------------GVQKQD---------------- 434
            + + L  I      P                       G   QD                
Sbjct: 1221 VEAMLRMIHFHKYDPKEKEEQRRQDLCLEQWEAEQNLNGHSDQDYSAQNLGGNHILQLKE 1280

Query: 435  ----VIELIMKDPFRTGFDCFLYGHECLSLLTFQKGNN-AKMKLPQGLCLNGSWMGFVTY 494
                 ++  +KD F T FD  +       L   Q   N   ++LP  L  N +W+GF   
Sbjct: 1281 SIPSFLQKDLKDRFGTTFDFVIPRRHIPQLFNQQSTKNYTAIELPPSLYTNSNWIGFAVC 1340

Query: 495  ALLTLNRPTTNDHEFKQPLKIVDLVCQFHADSDLIEPDHFLITSEDDLLEGGADQFIWLF 530
             L  +N+  T      +     +L+CQF  ++ LIEP H    +ED  +     QF+WL+
Sbjct: 1341 TLFQVNKHPTAILNNLRSASRHELICQFAVENGLIEPFHIHTITEDTFIWLHERQFVWLY 1400

BLAST of Lag0039426 vs. ExPASy TrEMBL
Match: A0A6J1EC12 (TMV resistance protein N-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432680 PE=4 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 3.9e-54
Identity = 170/604 (28.15%), Postives = 269/604 (44.54%), Query Frame = 0

Query: 15   FMIRLETDGYPPKCLRNVE-KECGMFSRAKVCVFYIPFEKLAEEVRNEDFRYIKASITSK 74
            F+IR+ETD  P K     +  +  + S   + VFY+P  ++  E        I  SI + 
Sbjct: 1081 FIIRMETDECPLKSALVFDGNKDELQSPVGLVVFYVPMRRI--EGWLNQCCCIDVSIMTD 1140

Query: 75   NPDIKVEKCGASVLFREDVVVFLRNMLNKCF-----------------QNRPIFTRI--- 134
            NP +KV+ CGAS+++ ++   F+  ++   F                 QNR   + +   
Sbjct: 1141 NPFVKVKWCGASIIYEQNAGSFIGKIIKGLFGSPGKYHTSIVDHILNRQNRVDVSSLVYG 1200

Query: 135  -----------------SKDRSHLSEGKYVEVEDPDQDDFSSTNEDSSSEHSIILLKRSL 194
                             S  R   S+     +ED      ++   + +     I+LKR+L
Sbjct: 1201 GARYKTSWLNALQRTIGSFPRLRASKPPPEAIEDGSTGMIAAAEAEETESDYSIMLKRNL 1260

Query: 195  KSIIRRTFEEENVRLAPLYMFPRKEMSISWFKHQQEQATLTIKLPLCLHKDKKWMGLALF 254
            K+++ RTFE+  +     Y+FPRKE+S SWF  Q ++  +TIK+P  LHKDKKWMGLA F
Sbjct: 1261 KAMLLRTFEDLKL-YGEFYVFPRKEISRSWFNLQLKKPKVTIKIPPNLHKDKKWMGLAFF 1320

Query: 255  VVFSVEGHLGRHHTFSYNIDGDEVQYAHSPSIKMPKYLVDIDSSQQVCVLFNSRASFSYA 314
            VVF V+ +    H+FSY ++ DE        + + K L   D S Q+ V F  RA + Y 
Sbjct: 1321 VVFGVDENSPNAHSFSYQVENDEYTMQRESILYLTKGL--FDDSHQLWVFFEPRAVYPYR 1380

Query: 315  LNCSDHIHANI--TTEPGVKVVSCGARLVYQQNVRGLIETIVDCVCQSPPYLHDYQTTMI 374
            LN   H+  +        +K V CGARL Y+ +V GLI T+++ V  SP  LH++   + 
Sbjct: 1381 LNQWRHLCVSFVCNNNSSLKAVVCGARLAYKHDVEGLINTMINNVMGSPADLHEFYDQVY 1440

Query: 375  LASF-----------------------------------------LTS-------ITALG 434
            + S                                          LTS       +  L 
Sbjct: 1441 VESMIRMIHFHKYDPKQKEAEGEDDLCLEELIEEHNSNGYPQDSTLTSNAMERNHLLELK 1500

Query: 435  DTPPPGVQKQDVIELIMKDPFRTGFDCFLYGHECLSLLTFQ-KGNNAKMKLPQGLCLNGS 494
            +T P  +QK       +KD F T FD  +           Q + N   ++LP  L  N  
Sbjct: 1501 ETIPSFLQKD------LKDRFGTTFDFVIPRRNIPEWFNQQSEKNQTAIQLPPSLYTNSD 1560

Query: 495  WMGFVTYALLTLNRPTTNDHEFKQPLKIVDLVCQFHADSDLIEPDHFLITSEDDLLEGGA 530
            WMGF   AL  +N+  T      + +   +L+CQF  ++ +I P H    +ED  +    
Sbjct: 1561 WMGFAVCALFQINKHPTAILNNLRSISRHELLCQFAVENGVIHPIHIHTVTEDRFIWLHE 1620

BLAST of Lag0039426 vs. ExPASy TrEMBL
Match: A0A6J1IBG2 (TMV resistance protein N-like OS=Cucurbita maxima OX=3661 GN=LOC111472024 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.9e-53
Identity = 167/599 (27.88%), Postives = 269/599 (44.91%), Query Frame = 0

Query: 15   FMIRLETDGYPPKCLRNVE-KECGMFSRAKVCVFYIPFEKLAEEVRNEDFRYIKASITSK 74
            F+IR+ETD  P K     +  +  + S   + VFY+P  ++  E        I  SI + 
Sbjct: 1065 FIIRMETDECPLKSALVFDGNKDELQSPVGLVVFYVPMRRI--EGWLNQCCCIDVSIVTD 1124

Query: 75   NPDIKVEKCGASVLFREDVVVFLRNMLNKCF-----------------QNRPIFTRI--- 134
            NP +KV+ CGAS+++ ++   F+  ++   F                 QNR   + +   
Sbjct: 1125 NPFVKVKWCGASIIYEQNAGSFIGKIIKALFGSPGKYHTSIVDHILNRQNRVDVSSLVDG 1184

Query: 135  -----------------SKDRSHLSEGKYVEVEDPDQDDFSSTNEDSSSEHSIILLKRSL 194
                             S  R   S+     +ED      ++   + +     I+LKR+L
Sbjct: 1185 GARYKTSWLNALQRTIGSFPRLRASKPPPEAIEDGSTSMIAAAEAEETESDYSIMLKRNL 1244

Query: 195  KSIIRRTFEEENVRLAPLYMFPRKEMSISWFKHQQEQATLTIKLPLCLHKDKKWMGLALF 254
            K+++ RTFE+  +     Y+FPRKE+S SWF  Q ++  +TIK+P  LHKDKKWMGLA F
Sbjct: 1245 KAMLLRTFEDLKL-YGEYYVFPRKEISRSWFNLQLKKPKVTIKIPPNLHKDKKWMGLAFF 1304

Query: 255  VVFSVEGHLGRHHTFSYNIDGDEVQYAHSPSIKMPKYLVDIDSSQQVCVLFNSRASFSYA 314
            VVF+V+ +    H+FSY ++ DE        + + K L   D   Q+ V F  RA + Y 
Sbjct: 1305 VVFAVDENSPNAHSFSYQVENDEYTMQRESILYLTKGL--FDDFHQLWVFFEPRAVYPYR 1364

Query: 315  LNCSDHIHANI--TTEPGVKVVSCGARLVYQQNVRGLIETIVDCVCQSPPYLHDYQTTMI 374
            LN   H+  +        +K V CGARL Y+ +V GLI T+++ V  SP  LH++   + 
Sbjct: 1365 LNQWRHLCVSFVCNNNSSLKAVVCGARLAYKHDVEGLINTMINSVMGSPADLHEFYDQVY 1424

Query: 375  LASFLTSI--------------------TALGDTPPPGVQKQD----------------- 434
            + S +  I                      L +        QD                 
Sbjct: 1425 VESMIKMIHFHKYDPKQKEFEREDDLCLEELTEEQNSNGYPQDSTLTSNAMERNHLLELK 1484

Query: 435  -----VIELIMKDPFRTGFDCFLYGHECLSLLTFQKG--NNAKMKLPQGLCLNGSWMGFV 494
                  +++ +KD F T FD F+     +     Q+   N   ++LP  L  N  WMGF 
Sbjct: 1485 EAIPSFLQMDLKDRFGTIFD-FVIPRRNIPEWFNQRSEKNQTGIQLPPSLYTNSDWMGFA 1544

Query: 495  TYALLTLNRPTTNDHEFKQPLKIVDLVCQFHADSDLIEPDHFLITSEDDLLEGGADQFIW 530
              AL  +N+  T      + +   +L+CQF  ++ +I P H    +ED  +     QF+W
Sbjct: 1545 VCALFQINKHPTAILNNLRSISRHELLCQFSVENGVIHPIHIHTITEDRFIWLHERQFLW 1604