Lag0039352 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0039352 vs. NCBI nr
Match: XP_022932490.1 (protein NRT1/ PTR FAMILY 4.4-like [Cucurbita moschata])

HSP 1 Score: 857.1 bits (2213), Expect = 8.9e-245
Identity = 461/585 (78.80%), Postives = 493/585 (84.27%), Query Frame = 0

Query: 1   MGDVLEKTKSKCKEMNSMSEDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60
           MGD LEKTK+K KE+NSM EDTSVDWRGRPC SNKHGGM AAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDALEKTKTKSKEINSMYEDTSVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFSEMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFG 120
           NNLITYVF++MHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGF+EL G
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120

Query: 121 FSLLTVQAHYEGLRPAGCEMGRGEEECVEAKGYEALMLYAALYLVALGSGCLKPNIISHG 180
           FSLLTVQAHY  LRP  C+MG G ++C+EAKGYEALML+ ALYLVALGSGCLKPNIISHG
Sbjct: 121 FSLLTVQAHYPRLRPPACDMGTG-QQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHG 180

Query: 181 ADQFPKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLG 240
           ADQF +Q PTQF+KLSTFFNCAYFAFCTGELIALTLLVW+QTH GMD+GF VSTAAMLLG
Sbjct: 181 ADQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLG 240

Query: 241 LISLLSGTSSFRNSPPRGTIFTPIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLHTD 300
           LISLL+GTS FRNSPPRGTIFTPIAQVFVAAF KRKQICPSNSEMLH SQNGVVKLLHTD
Sbjct: 241 LISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTD 300

Query: 301 KFR------RESGKGRKSMETLHSNTSGTSENN------SISDPHIC--LHHNLQHHLST 360
            FR       +S +GR   E         ++        S+     C  + + +   L T
Sbjct: 301 NFRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQT 360

Query: 361 APNLFSPTRSCHEHMAHQLLPNPPASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGI 420
                    +     + Q+   PPASLQSIPY+ILIFLVPLYET FVPLARR+T T+SGI
Sbjct: 361 FSVQQGAAMNTSLTNSFQI---PPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGI 420

Query: 421 SPLQRVGTGLFVATFSMVSAALVEQKRRNSSAAGSSLSILWIAPQFVIFGISELFTAVGL 480
           SPLQRVGTGLFVATFSMVSAALVE+KRRNSS  GSSLSILWIAPQF+IFGISELFTAVGL
Sbjct: 421 SPLQRVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGL 480

Query: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHNDLNNDR 540
           VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIT G SGGGWLSHNDLNNDR
Sbjct: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGASGGGWLSHNDLNNDR 540

Query: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQQQHLVAVDLENQTSKPS 572
           LDLFYWLLAGLSLVNFFNYLFWANRYSQ+    A DLEN TSKPS
Sbjct: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQEHE--AQDLENPTSKPS 579

BLAST of Lag0039352 vs. NCBI nr
Match: XP_022973463.1 (protein NRT1/ PTR FAMILY 4.4-like [Cucurbita maxima])

HSP 1 Score: 856.7 bits (2212), Expect = 1.2e-244
Identity = 461/585 (78.80%), Postives = 494/585 (84.44%), Query Frame = 0

Query: 1   MGDVLEKTKSKCKEMNSMSEDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60
           MGD LEKTK+K KE+ SMSEDTSVDWRGRPC SNKHGGMTAAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDALEKTKTKSKELTSMSEDTSVDWRGRPCTSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFSEMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFG 120
           NNLITYVF++MHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGF+EL G
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120

Query: 121 FSLLTVQAHYEGLRPAGCEMGRGEEECVEAKGYEALMLYAALYLVALGSGCLKPNIISHG 180
           FSLLTVQAHY  LRP  C+MG G ++C+EAKGYEALML+ ALYLVALGSGCLKPNIISHG
Sbjct: 121 FSLLTVQAHYPRLRPLACDMGTG-QQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHG 180

Query: 181 ADQFPKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLG 240
           ADQF +Q PTQF+KLSTFFNCAYFAFCTGEL+ALTLLVW+QTH GMD+GF VSTAAMLLG
Sbjct: 181 ADQFRRQHPTQFQKLSTFFNCAYFAFCTGELLALTLLVWVQTHYGMDVGFTVSTAAMLLG 240

Query: 241 LISLLSGTSSFRNSPPRGTIFTPIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLHTD 300
           LISLL+GTS FRNSPPRGTIFTPIAQVFVAAF KRKQI PSNSEMLH SQNGVVKLLHTD
Sbjct: 241 LISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQIYPSNSEMLHRSQNGVVKLLHTD 300

Query: 301 KFR------RESGKGRKSMETLHSNTSGTSENN------SISDPHIC--LHHNLQHHLST 360
           KFR       +S +GR   E         ++        S+     C  + + +   L T
Sbjct: 301 KFRFLDKACIKSEEGRVGSEESQWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQT 360

Query: 361 APNLFSPTRSCHEHMAHQLLPNPPASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGI 420
                    +     + Q+   PPASLQSIPY+ILIFLVPLYET FVPLARR+T T+SGI
Sbjct: 361 FSVQQGAAMNTCLTNSFQI---PPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGI 420

Query: 421 SPLQRVGTGLFVATFSMVSAALVEQKRRNSSAAGSSLSILWIAPQFVIFGISELFTAVGL 480
           SPLQRVGTGLFVATFSMVSAALVE+KRRNSS  GSSLSILWIAPQF+IFGISELFTAVGL
Sbjct: 421 SPLQRVGTGLFVATFSMVSAALVEKKRRNSSVVGSSLSILWIAPQFIIFGISELFTAVGL 480

Query: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHNDLNNDR 540
           VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIT G SGGGWLSHNDLNNDR
Sbjct: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGASGGGWLSHNDLNNDR 540

Query: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQQQHLVAVDLENQTSKPS 572
           LDLFYWLLAGLSLVNFFNYLFWANRYSQ+    A DLEN TSKPS
Sbjct: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQEHE--AQDLENPTSKPS 579

BLAST of Lag0039352 vs. NCBI nr
Match: KAG7037184.1 (Protein NRT1/ PTR FAMILY 4.4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 853.6 bits (2204), Expect = 9.8e-244
Identity = 459/585 (78.46%), Postives = 492/585 (84.10%), Query Frame = 0

Query: 1   MGDVLEKTKSKCKEMNSMSEDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60
           MGD LE+TK+K KE+NSM EDT VDWRGRPC SNKHGGM AAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDALERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFSEMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFG 120
           NNLITYVF++MHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGF+EL G
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120

Query: 121 FSLLTVQAHYEGLRPAGCEMGRGEEECVEAKGYEALMLYAALYLVALGSGCLKPNIISHG 180
           FSLLTVQAHY  LRP  C+MG G ++C+EAKGYEALML+ ALYLVALGSGCLKPNIISHG
Sbjct: 121 FSLLTVQAHYPRLRPPACDMGTG-QQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHG 180

Query: 181 ADQFPKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLG 240
           ADQF +Q PTQF+KLSTFFNCAYFAFCTGELIALTLLVW+QTH GMD+GF VSTAAMLLG
Sbjct: 181 ADQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLG 240

Query: 241 LISLLSGTSSFRNSPPRGTIFTPIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLHTD 300
           LISLL+GTS FRNSPPRGTIFTPIAQVFVAAF KRKQICPSNSEMLH SQNGVVKLLHTD
Sbjct: 241 LISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTD 300

Query: 301 KFR------RESGKGRKSMETLHSNTSGTSENN------SISDPHIC--LHHNLQHHLST 360
            FR       +S +GR   E         ++        S+     C  + + +   L T
Sbjct: 301 NFRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQT 360

Query: 361 APNLFSPTRSCHEHMAHQLLPNPPASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGI 420
                    +     + Q+   PPASLQSIPY+ILIFLVPLYET FVPLARR+T T+SGI
Sbjct: 361 FSVQQGAAMNTSLTNSFQI---PPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGI 420

Query: 421 SPLQRVGTGLFVATFSMVSAALVEQKRRNSSAAGSSLSILWIAPQFVIFGISELFTAVGL 480
           SPLQRVGTGLFVATFSMVSAALVE+KRRNSS  GSSLSILWIAPQF+IFGISELFTAVGL
Sbjct: 421 SPLQRVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGL 480

Query: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHNDLNNDR 540
           VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIT G SGGGWLSHNDLNNDR
Sbjct: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDR 540

Query: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQQQHLVAVDLENQTSKPS 572
           LDLFYWLLAGLSLVNFFNYLFWANRYSQ+    A DLEN TSKPS
Sbjct: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQEHE--AQDLENPTSKPS 579

BLAST of Lag0039352 vs. NCBI nr
Match: XP_023523828.1 (protein NRT1/ PTR FAMILY 4.4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 844.7 bits (2181), Expect = 4.6e-241
Identity = 457/585 (78.12%), Postives = 491/585 (83.93%), Query Frame = 0

Query: 1   MGDVLEKTKSKCKEMNSMSEDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60
           MGD LEKTK+K K++ SMSEDTSVDWRGRPC S+KHGGM AAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDALEKTKTKSKQITSMSEDTSVDWRGRPCTSDKHGGMAAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFSEMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFG 120
           NNLITYVF++MHFPLSKSANVVTNFVGTVF LSLLGGFLSDSYLGSFRTMLIFGF+EL G
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFLLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120

Query: 121 FSLLTVQAHYEGLRPAGCEMGRGEEECVEAKGYEALMLYAALYLVALGSGCLKPNIISHG 180
           FSL+TVQAHY  LRP  C+MG G ++C+EAKGYEALML+ ALYLVALGSGCLKPNIISHG
Sbjct: 121 FSLVTVQAHYPRLRPPACDMGTG-QQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHG 180

Query: 181 ADQFPKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLG 240
           ADQF +Q PTQF+KLSTFFNCAYFAFCTGELIALTLLVW+QTH GMD+GF VSTAAMLLG
Sbjct: 181 ADQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLG 240

Query: 241 LISLLSGTSSFRNSPPRGTIFTPIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLHTD 300
           LISLL+GTS FRNSPPRGTIFTPIAQVFVAAF KRKQICPSNSEMLH SQN VVKLLHTD
Sbjct: 241 LISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNAVVKLLHTD 300

Query: 301 KF--------RRESGK--GRKSMETLHSNTSGTSENNSISDPHICLHHNLQHHLSTAPNL 360
           KF        + E G+    +S   L + T        +S   I     + + +      
Sbjct: 301 KFTFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQT 360

Query: 361 FSPTRSCHEHMAHQLLPN----PPASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGI 420
           FS  +       +  L N    PPASLQSIPY+ILIFLVPLYET FVPLARR+T T+SGI
Sbjct: 361 FSVQQGA---AMNTCLTNSFKIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGI 420

Query: 421 SPLQRVGTGLFVATFSMVSAALVEQKRRNSSAAGSSLSILWIAPQFVIFGISELFTAVGL 480
           SPLQRVGTGLFVATFSMVSAALVE+KRRNSS  GSSLSILWIAPQF+IFGISELFTAVGL
Sbjct: 421 SPLQRVGTGLFVATFSMVSAALVEKKRRNSSLVGSSLSILWIAPQFIIFGISELFTAVGL 480

Query: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHNDLNNDR 540
           VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIT   SGGGWLSHNDLNNDR
Sbjct: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGEASGGGWLSHNDLNNDR 540

Query: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQQQHLVAVDLENQTSKPS 572
           LDLFYWLLAGLSLVNFFNYLFWANRYSQ+    A DLEN TSKPS
Sbjct: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQEHD--AQDLENPTSKPS 579

BLAST of Lag0039352 vs. NCBI nr
Match: XP_022974070.1 (protein NRT1/ PTR FAMILY 4.4-like [Cucurbita maxima])

HSP 1 Score: 833.9 bits (2153), Expect = 8.1e-238
Identity = 454/573 (79.23%), Postives = 482/573 (84.12%), Query Frame = 0

Query: 1   MGDVLEKTKSKCKEMNSMSEDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60
           MGD LEKTKS       +S DT VDWRGRPCNSNKHGGM AAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDSLEKTKS------ILSVDTCVDWRGRPCNSNKHGGMGAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFSEMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFG 120
           NNLITYV+SEMHFPLSK+ANVVTNFVGTVF LSLLGGFLSDSYLGSFRTMLIFGF+EL G
Sbjct: 61  NNLITYVYSEMHFPLSKAANVVTNFVGTVFLLSLLGGFLSDSYLGSFRTMLIFGFIELSG 120

Query: 121 FSLLTVQAHYEGLRPAGCEMGRGEE--ECVEAKGYEALMLYAALYLVALGSGCLKPNIIS 180
           F++LTVQAHY GLRPA CEMGR  E  EC EAKGY+ALM ++ALYLVALGSGCLKPNIIS
Sbjct: 121 FTVLTVQAHYPGLRPAACEMGRSGEGDECEEAKGYKALMFFSALYLVALGSGCLKPNIIS 180

Query: 181 HGADQFPKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAML 240
           HGADQF KQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVW+QTHSGMDLGFGVSTAAML
Sbjct: 181 HGADQFQKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAML 240

Query: 241 LGLISLLSGTSSFRNSPPRGTIFTPIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLH 300
           L LISLLSGTS FRN+PPRG+IFTPIAQVFVAAFTKRKQICPSNSEMLHGS NGVVKLLH
Sbjct: 241 LALISLLSGTSFFRNNPPRGSIFTPIAQVFVAAFTKRKQICPSNSEMLHGSHNGVVKLLH 300

Query: 301 TDKFRRESGKGRKSMETLHSNTSGTSENNSISDPHICLHHNLQHHLSTAPNLFSPTRSCH 360
           TD FR       KS ET     +GT E +      +     ++  LS  P +F+ T   +
Sbjct: 301 TDNFRFLDKACIKSEET----RAGTREESPWKLCTVTQVEQVKIILSVVP-IFACTIIFN 360

Query: 361 EHMA-------------HQLLPN----PPASLQSIPYLILIFLVPLYETVFVPLARRLTG 420
             +A             +  L N    PPASLQSIPYLILIFLVPLYET FVPLARRLT 
Sbjct: 361 TILAQLQTFSVQQGASMNTTLTNSFQIPPASLQSIPYLILIFLVPLYETAFVPLARRLTA 420

Query: 421 TDSGISPLQRVGTGLFVATFSMVSAALVEQKRRNSSAAGSSLSILWIAPQFVIFGISELF 480
           TDSGI+PLQRVGTGLFVATFSMVSAALVEQKRR+S    S+LSILWIAPQFVIFGISELF
Sbjct: 421 TDSGITPLQRVGTGLFVATFSMVSAALVEQKRRHS----SNLSILWIAPQFVIFGISELF 480

Query: 481 TAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHND 540
           TAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVS+VNK++RG SGGGWLSHND
Sbjct: 481 TAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSVVNKVSRGESGGGWLSHND 540

Query: 541 LNNDRLDLFYWLLAGLSLVNFFNYLFWANRYSQ 555
           LN DRLDLFYWLLAGLSLVNFFNYLFWANRYSQ
Sbjct: 541 LNKDRLDLFYWLLAGLSLVNFFNYLFWANRYSQ 558

BLAST of Lag0039352 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 4.8e-185
Identity = 356/556 (64.03%), Postives = 413/556 (74.28%), Query Frame = 0

Query: 20  EDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSA 79
           +D  VD+RGRPC  +KHGG  AA FVLG QAFEMMAIAAVGNNLITYVF+EMHFPLSKSA
Sbjct: 23  DDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSA 82

Query: 80  NVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFGFSLLTVQAHYEGLRPAGCE 139
           N+VTNF+GTVF LSLLGGFLSDSYLGSFRTML+FG +E+ GF LL+VQAH   LRP  C 
Sbjct: 83  NLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECN 142

Query: 140 MGRGEEECVEAKGYEALMLYAALYLVALGSGCLKPNIISHGADQFPKQDPTQFKKLSTFF 199
           M      CVEA GY+A  LY AL LVALGSGCLKPNIISHGA+QF ++D    +KLS+FF
Sbjct: 143 MKSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFF 202

Query: 200 NCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLGLISLLSGTSSFRNSPPRGT 259
           N AYFAF  G+LIALTLLVW+QTHSGMD+GFGVS A M  G+ISL++GTS +RN PP G+
Sbjct: 203 NAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGS 262

Query: 260 IFTPIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVK---LLHTDKFR-------RESGKG 319
           IFTPIAQVFVAA TKRKQICPSN  M+H     +V+   LLH++KFR       +  GK 
Sbjct: 263 IFTPIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHSNKFRFLDKACIKTQGKA 322

Query: 320 RKS------METLHSNTSGTSENNSISDPHIC--LHHNLQHHLSTAPNLFSPTRSCHEHM 379
            +S      +E +H          S+     C  + + +   L T       + + H   
Sbjct: 323 MESPWRLCTIEQVHQ----VKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITK 382

Query: 380 AHQLLPNPPASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGISPLQRVGTGLFVATF 439
             Q+   PPASLQ+IPY+ILIF VPLYET FVPLAR+LTG DSGISPLQR+GTGLF+ATF
Sbjct: 383 TFQI---PPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATF 442

Query: 440 SMVSAALVEQKRRNSSAAGS-SLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQS 499
           SMV+AALVE+KRR S    +  LSI WIAPQF+IFG+SE+FTAVGLVEFFYKQS + MQS
Sbjct: 443 SMVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQS 502

Query: 500 FLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGG---GWLSHNDLNNDRLDLFYWLLAGL 554
           FLTAMTYCSYSFGFYLSS+LVS VN++T     G   GWL  NDLN DRLD FYWLLA L
Sbjct: 503 FLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASL 562

BLAST of Lag0039352 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 1.7e-169
Identity = 330/549 (60.11%), Postives = 401/549 (73.04%), Query Frame = 0

Query: 18  MSEDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSK 77
           ++E+ SVDWRGRP N NKHGGM AA FVLGLQAFE+M IAAVGNNLITYV +EMHFPLSK
Sbjct: 24  LAEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSK 83

Query: 78  SANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFGFSLLTVQAHYEGLRPAG 137
           +AN+VTNFVGT+F  +LLGG+LSD++LGSF T++IFGFVEL GF LL+VQAH   L+P  
Sbjct: 84  AANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPK 143

Query: 138 CEMGRGEEECVEAKGYEALMLYAALYLVALGSGCLKPNIISHGADQFPKQDPTQFKKLST 197
           C     ++ C EAKG++A++ + ALYLVALGSGC+KPN+I+HGADQF +  P Q K+LS+
Sbjct: 144 CN-PLIDQTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSS 203

Query: 198 FFNCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLGLISLLSGTSSFRNSPPR 257
           +FN AYFAF  GELIALTLLVW+QTHSGMD+GFGVS AAM +G+ISL+SGT  FRN  PR
Sbjct: 204 YFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPR 263

Query: 258 GTIFTPIAQVFVAAFTKRKQICPSNSEMLHGSQ---NGVV---KLLHTDKFR-------- 317
            +IFTPIA V VAA  KRK   PS+  MLHG     N VV    L HT +FR        
Sbjct: 264 RSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIK 323

Query: 318 RESGKGRKSMETLHSNTSGTSENNSISDPHICLHHNLQHHLSTAPNLFSPTR-SCHEHMA 377
            +    ++S   L + T        IS   I     + + +      FS  + S      
Sbjct: 324 IQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRL 383

Query: 378 HQLLPNPPASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGISPLQRVGTGLFVATFS 437
                 PPASLQ+IPY++LIFLVPLY++  VP AR+LTG +SGI PL R+G GLF++TFS
Sbjct: 384 SNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFS 443

Query: 438 MVSAALVEQKRRNSSAA-GSSLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSF 497
           MVSAA++E+KRR+SS   G  LSI WI PQF+IFGISE+FTAVGL+EFFYKQS +GM+SF
Sbjct: 444 MVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESF 503

Query: 498 LTAMTYCSYSFGFYLSSLLVSLVNKITRGG-SGGGWLSHNDLNNDRLDLFYWLLAGLSLV 550
           L A+TYCSYSFGFY SS+LVS+VNKIT       GWL  NDLN DRLDLFYWLLA LSL+
Sbjct: 504 LMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLL 563

BLAST of Lag0039352 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 5.9e-90
Identity = 212/577 (36.74%), Postives = 307/577 (53.21%), Query Frame = 0

Query: 25  DWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVTN 84
           DWR R     +HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH   SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 85  FVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFGFSLLTVQAHYEGLRPAGCEMGRGE 144
           F+GT F L+LLGGFLSD++  +F+  LI   +E  G  +LT+QA    L P  C+     
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCD----S 134

Query: 145 EECVEAKGYEALMLYAALYLVALGSGCLKPNIISHGADQFPKQDPTQFKKLSTFFNCAYF 204
             C E  G +A ML+  LYLVALG G +K ++ SHGA+QF +  P   K+ STFFN  YF
Sbjct: 135 PTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFN--YF 194

Query: 205 AFC--TGELIALTLLVWLQTHSGMDLGFGVSTAAMLLGLISLLSGTSSFRNSPPRGTIFT 264
            FC   G L+A+T +VWL+ + G + GFGVST A+ + ++  LSG+  +RN  P G+  T
Sbjct: 195 VFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLT 254

Query: 265 PIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLHTDKFRRESGKGRKSMETLHSNTSG 324
            I +V +AA  K    C S+     GS +  V  +          KG+K +E+       
Sbjct: 255 TILKVLLAASVK----CCSS-----GSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKP 314

Query: 325 TSEN---------------NSISDP---HICLHHNLQH------HLSTAP---------- 384
             E                N  +D    H  L   +Q        L   P          
Sbjct: 315 RQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNC 374

Query: 385 -----NLFSPTRSCHEHMAHQLLPNPPASLQSIPYLILIFLVPLYETVFVPLARRLTGTD 444
                + FS  ++   +     L  PPASL   P + ++ L P+Y+ + +P AR+ T T+
Sbjct: 375 CLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTE 434

Query: 445 SGISPLQRVGTGLFVATFSMVSAALVEQKRRN--------SSAAGSSLSILWIAPQFVIF 504
           +G++ LQR+G GL ++  +M  AALVE KR+          S     ++ LWIA Q++  
Sbjct: 435 TGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFL 494

Query: 505 GISELFTAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGG 553
           G ++LFT  GL+E+F+ ++   M+S  T++++ S + G+YLSS++VS+VN IT       
Sbjct: 495 GSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTP 554

BLAST of Lag0039352 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 4.8e-84
Identity = 197/550 (35.82%), Postives = 304/550 (55.27%), Query Frame = 0

Query: 25  DWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVTN 84
           DWR +     +HGGM AA+FVL ++  E +A  A  +NL+ Y+ + MH  L++S++ VT 
Sbjct: 17  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 76

Query: 85  FVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFGFSLLTVQAHYEGLRPAGCEMGRGE 144
           F+ T F L+LLGGFL+D++  +F   LI   +E  G  LLT+QA    L P  C+     
Sbjct: 77  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCK-SSAA 136

Query: 145 EECVEAKGYEALMLYAALYLVALGSGCLKPNIISHGADQFPKQDPTQFKKLSTFFNCAYF 204
             C    G +A  L+  LYLV+LG G +K ++ SHGA+QF +  P   K+ STFFN   F
Sbjct: 137 LRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVF 196

Query: 205 AFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLGLISLLSGTSSFRNSPPRGTIFTPI 264
               G L+A+T +VW++ + G + GFGVST ++ L ++  L G+  ++N  PRG+  T I
Sbjct: 197 CLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTI 256

Query: 265 AQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLHTDKFRRESGKGRKSMETLHSNTSGTS 324
            +V +AA       C S +   H +    V+  H +K   +S     S+  L+    G +
Sbjct: 257 FKVLLAASIVS---CSSKTSSNHFTSR-EVQSEHEEKTPSQS--LTNSLTCLNKAIEGKT 316

Query: 325 EN-------NSISDPHICLHHNLQHHLSTAPNLFSPTRSCHE-HMA---HQLLPN---PP 384
            +         + D  I L        +   N      S +  H A   ++ + N   P 
Sbjct: 317 HHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPS 376

Query: 385 ASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGISPLQRVGTGLFVATFSMVSAALVE 444
           ASL   P + ++ L P Y+ + +P AR++T ++ GI+ LQR+G GL ++  +M  AALVE
Sbjct: 377 ASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVE 436

Query: 445 QKRRN--------SSAAGSSLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFL 504
            KR+          S     ++ LWIA Q++  G ++LFT  GL+EFF+ ++   M+S  
Sbjct: 437 LKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLA 496

Query: 505 TAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHNDLNNDRLDLFYWLLAGLSLVNF 553
           T++++ S + G+YLSS++V +VN++T+      WL    LN +RLDLFYWL+  LS+VNF
Sbjct: 497 TSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGEK-LNRNRLDLFYWLMCVLSVVNF 556

BLAST of Lag0039352 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 301.2 bits (770), Expect = 2.5e-80
Identity = 198/558 (35.48%), Postives = 304/558 (54.48%), Query Frame = 0

Query: 24  VDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVT 83
           VDWR RP    +HGGM AA+FVL ++  E +A  A  +NL+ Y+ ++M F  S +AN VT
Sbjct: 45  VDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTKMGFSPSGAANAVT 104

Query: 84  NFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFGFSLLTVQAHYEGLRPAGCEMGRG 143
            F+GT FFL+LLGGFL+D++  +F   L+   +E  G  +LTVQAH     P        
Sbjct: 105 AFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEHSTEP-------- 164

Query: 144 EEECVEAKGYEALMLYAALYLVALGSGCLKPNIISHGADQFPKQDPTQFKKLSTFFNCAY 203
                    +  + L+  LYLVALG G +K ++  HGA+QF ++  +  ++ S FFN   
Sbjct: 165 ---------WSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRSFFFNYFI 224

Query: 204 FAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLGLISLLSGTSSFRNSPPRGTIFTP 263
           F+   G LIA+T++VWL+ + G   GFGVSTAA+L+ +   L+G+  +R   P G+  T 
Sbjct: 225 FSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVPSGSPITT 284

Query: 264 IAQVFVAA-FTKRKQICPSNSEMLHGSQNGVVKLLHTDKFRRES-------GKGRKSMET 323
           + +V  AA + K K+   S   +   ++N     +       +        G+  +  E+
Sbjct: 285 LFKVLTAALYAKYKKRRTSRIVVTCHTRNDCDDSVTKQNCDGDDGFLGSFLGEVVRERES 344

Query: 324 LHSNTSGTSENNSISDPHICLHHNLQHHLSTAP--------NLFSPTRSCHEHMAHQLLP 383
           L      T E   + D  I +   L   +ST          + FS  ++   +       
Sbjct: 345 LPRPLRCTEE--QVKDVKIVI-KILPIFMSTIMLNCCLAQLSTFSVQQASTMNTKLGSFT 404

Query: 384 NPPASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGISPLQRVGTGLFVATFSMVSAA 443
            PPA+L   P + ++ L P Y  + +PLAR+ T T++GI+ LQR+GTGL ++  +M  AA
Sbjct: 405 VPPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGITHLQRIGTGLVLSIVAMAVAA 464

Query: 444 LVEQKRR----------NSSAAGSS---LSILWIAPQFVIFGISELFTAVGLVEFFYKQS 503
           LVE KR+          NSS+  SS   ++ LW+A Q+V  G ++LFT  G++EFF+ ++
Sbjct: 465 LVETKRKHVVVSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGSADLFTLAGMMEFFFTEA 524

Query: 504 VEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHNDLNNDRLDLFYWLL 553
              M+S  T++++ S + G+Y SS+LVS VN +T       WL   +LN   L+ FYWL+
Sbjct: 525 PSTMRSLATSLSWASLAMGYYFSSVLVSAVNFVTGLNHHNPWLLGENLNQYHLERFYWLM 582

BLAST of Lag0039352 vs. ExPASy TrEMBL
Match: A0A6J1F2C4 (protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita moschata OX=3662 GN=LOC111438893 PE=3 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 4.3e-245
Identity = 461/585 (78.80%), Postives = 493/585 (84.27%), Query Frame = 0

Query: 1   MGDVLEKTKSKCKEMNSMSEDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60
           MGD LEKTK+K KE+NSM EDTSVDWRGRPC SNKHGGM AAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDALEKTKTKSKEINSMYEDTSVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFSEMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFG 120
           NNLITYVF++MHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGF+EL G
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120

Query: 121 FSLLTVQAHYEGLRPAGCEMGRGEEECVEAKGYEALMLYAALYLVALGSGCLKPNIISHG 180
           FSLLTVQAHY  LRP  C+MG G ++C+EAKGYEALML+ ALYLVALGSGCLKPNIISHG
Sbjct: 121 FSLLTVQAHYPRLRPPACDMGTG-QQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHG 180

Query: 181 ADQFPKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLG 240
           ADQF +Q PTQF+KLSTFFNCAYFAFCTGELIALTLLVW+QTH GMD+GF VSTAAMLLG
Sbjct: 181 ADQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLG 240

Query: 241 LISLLSGTSSFRNSPPRGTIFTPIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLHTD 300
           LISLL+GTS FRNSPPRGTIFTPIAQVFVAAF KRKQICPSNSEMLH SQNGVVKLLHTD
Sbjct: 241 LISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTD 300

Query: 301 KFR------RESGKGRKSMETLHSNTSGTSENN------SISDPHIC--LHHNLQHHLST 360
            FR       +S +GR   E         ++        S+     C  + + +   L T
Sbjct: 301 NFRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQT 360

Query: 361 APNLFSPTRSCHEHMAHQLLPNPPASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGI 420
                    +     + Q+   PPASLQSIPY+ILIFLVPLYET FVPLARR+T T+SGI
Sbjct: 361 FSVQQGAAMNTSLTNSFQI---PPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGI 420

Query: 421 SPLQRVGTGLFVATFSMVSAALVEQKRRNSSAAGSSLSILWIAPQFVIFGISELFTAVGL 480
           SPLQRVGTGLFVATFSMVSAALVE+KRRNSS  GSSLSILWIAPQF+IFGISELFTAVGL
Sbjct: 421 SPLQRVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGL 480

Query: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHNDLNNDR 540
           VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIT G SGGGWLSHNDLNNDR
Sbjct: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGASGGGWLSHNDLNNDR 540

Query: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQQQHLVAVDLENQTSKPS 572
           LDLFYWLLAGLSLVNFFNYLFWANRYSQ+    A DLEN TSKPS
Sbjct: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQEHE--AQDLENPTSKPS 579

BLAST of Lag0039352 vs. ExPASy TrEMBL
Match: A0A6J1ID44 (protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita maxima OX=3661 GN=LOC111472001 PE=3 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 5.6e-245
Identity = 461/585 (78.80%), Postives = 494/585 (84.44%), Query Frame = 0

Query: 1   MGDVLEKTKSKCKEMNSMSEDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60
           MGD LEKTK+K KE+ SMSEDTSVDWRGRPC SNKHGGMTAAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDALEKTKTKSKELTSMSEDTSVDWRGRPCTSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFSEMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFG 120
           NNLITYVF++MHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGF+EL G
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120

Query: 121 FSLLTVQAHYEGLRPAGCEMGRGEEECVEAKGYEALMLYAALYLVALGSGCLKPNIISHG 180
           FSLLTVQAHY  LRP  C+MG G ++C+EAKGYEALML+ ALYLVALGSGCLKPNIISHG
Sbjct: 121 FSLLTVQAHYPRLRPLACDMGTG-QQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHG 180

Query: 181 ADQFPKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLG 240
           ADQF +Q PTQF+KLSTFFNCAYFAFCTGEL+ALTLLVW+QTH GMD+GF VSTAAMLLG
Sbjct: 181 ADQFRRQHPTQFQKLSTFFNCAYFAFCTGELLALTLLVWVQTHYGMDVGFTVSTAAMLLG 240

Query: 241 LISLLSGTSSFRNSPPRGTIFTPIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLHTD 300
           LISLL+GTS FRNSPPRGTIFTPIAQVFVAAF KRKQI PSNSEMLH SQNGVVKLLHTD
Sbjct: 241 LISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQIYPSNSEMLHRSQNGVVKLLHTD 300

Query: 301 KFR------RESGKGRKSMETLHSNTSGTSENN------SISDPHIC--LHHNLQHHLST 360
           KFR       +S +GR   E         ++        S+     C  + + +   L T
Sbjct: 301 KFRFLDKACIKSEEGRVGSEESQWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQT 360

Query: 361 APNLFSPTRSCHEHMAHQLLPNPPASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGI 420
                    +     + Q+   PPASLQSIPY+ILIFLVPLYET FVPLARR+T T+SGI
Sbjct: 361 FSVQQGAAMNTCLTNSFQI---PPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGI 420

Query: 421 SPLQRVGTGLFVATFSMVSAALVEQKRRNSSAAGSSLSILWIAPQFVIFGISELFTAVGL 480
           SPLQRVGTGLFVATFSMVSAALVE+KRRNSS  GSSLSILWIAPQF+IFGISELFTAVGL
Sbjct: 421 SPLQRVGTGLFVATFSMVSAALVEKKRRNSSVVGSSLSILWIAPQFIIFGISELFTAVGL 480

Query: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHNDLNNDR 540
           VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIT G SGGGWLSHNDLNNDR
Sbjct: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGASGGGWLSHNDLNNDR 540

Query: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQQQHLVAVDLENQTSKPS 572
           LDLFYWLLAGLSLVNFFNYLFWANRYSQ+    A DLEN TSKPS
Sbjct: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQEHE--AQDLENPTSKPS 579

BLAST of Lag0039352 vs. ExPASy TrEMBL
Match: A0A6J1ID04 (protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita maxima OX=3661 GN=LOC111472692 PE=3 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.9e-238
Identity = 454/573 (79.23%), Postives = 482/573 (84.12%), Query Frame = 0

Query: 1   MGDVLEKTKSKCKEMNSMSEDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60
           MGD LEKTKS       +S DT VDWRGRPCNSNKHGGM AAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDSLEKTKS------ILSVDTCVDWRGRPCNSNKHGGMGAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFSEMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFG 120
           NNLITYV+SEMHFPLSK+ANVVTNFVGTVF LSLLGGFLSDSYLGSFRTMLIFGF+EL G
Sbjct: 61  NNLITYVYSEMHFPLSKAANVVTNFVGTVFLLSLLGGFLSDSYLGSFRTMLIFGFIELSG 120

Query: 121 FSLLTVQAHYEGLRPAGCEMGRGEE--ECVEAKGYEALMLYAALYLVALGSGCLKPNIIS 180
           F++LTVQAHY GLRPA CEMGR  E  EC EAKGY+ALM ++ALYLVALGSGCLKPNIIS
Sbjct: 121 FTVLTVQAHYPGLRPAACEMGRSGEGDECEEAKGYKALMFFSALYLVALGSGCLKPNIIS 180

Query: 181 HGADQFPKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAML 240
           HGADQF KQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVW+QTHSGMDLGFGVSTAAML
Sbjct: 181 HGADQFQKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAML 240

Query: 241 LGLISLLSGTSSFRNSPPRGTIFTPIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLH 300
           L LISLLSGTS FRN+PPRG+IFTPIAQVFVAAFTKRKQICPSNSEMLHGS NGVVKLLH
Sbjct: 241 LALISLLSGTSFFRNNPPRGSIFTPIAQVFVAAFTKRKQICPSNSEMLHGSHNGVVKLLH 300

Query: 301 TDKFRRESGKGRKSMETLHSNTSGTSENNSISDPHICLHHNLQHHLSTAPNLFSPTRSCH 360
           TD FR       KS ET     +GT E +      +     ++  LS  P +F+ T   +
Sbjct: 301 TDNFRFLDKACIKSEET----RAGTREESPWKLCTVTQVEQVKIILSVVP-IFACTIIFN 360

Query: 361 EHMA-------------HQLLPN----PPASLQSIPYLILIFLVPLYETVFVPLARRLTG 420
             +A             +  L N    PPASLQSIPYLILIFLVPLYET FVPLARRLT 
Sbjct: 361 TILAQLQTFSVQQGASMNTTLTNSFQIPPASLQSIPYLILIFLVPLYETAFVPLARRLTA 420

Query: 421 TDSGISPLQRVGTGLFVATFSMVSAALVEQKRRNSSAAGSSLSILWIAPQFVIFGISELF 480
           TDSGI+PLQRVGTGLFVATFSMVSAALVEQKRR+S    S+LSILWIAPQFVIFGISELF
Sbjct: 421 TDSGITPLQRVGTGLFVATFSMVSAALVEQKRRHS----SNLSILWIAPQFVIFGISELF 480

Query: 481 TAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHND 540
           TAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVS+VNK++RG SGGGWLSHND
Sbjct: 481 TAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSVVNKVSRGESGGGWLSHND 540

Query: 541 LNNDRLDLFYWLLAGLSLVNFFNYLFWANRYSQ 555
           LN DRLDLFYWLLAGLSLVNFFNYLFWANRYSQ
Sbjct: 541 LNKDRLDLFYWLLAGLSLVNFFNYLFWANRYSQ 558

BLAST of Lag0039352 vs. ExPASy TrEMBL
Match: A0A6J1EUC1 (protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita moschata OX=3662 GN=LOC111436690 PE=3 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 4.8e-236
Identity = 451/573 (78.71%), Postives = 478/573 (83.42%), Query Frame = 0

Query: 1   MGDVLEKTKSKCKEMNSMSEDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60
           MGD LEKTKS       +S DT VDWRGRPCNSNKHGGM AAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDSLEKTKS------ILSVDTCVDWRGRPCNSNKHGGMGAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFSEMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFG 120
           NNLITYV+SEMHFPLSK+ANVVTNFVGTVF LSLLGGFLSDSYLGSFRTMLIFGF+EL G
Sbjct: 61  NNLITYVYSEMHFPLSKAANVVTNFVGTVFLLSLLGGFLSDSYLGSFRTMLIFGFIELSG 120

Query: 121 FSLLTVQAHYEGLRPAGCEMGRGEE--ECVEAKGYEALMLYAALYLVALGSGCLKPNIIS 180
           F++LTVQAHY GLRPA CEMG   E  EC EAKGY+ALM ++ALYLVALGSGCLKPNIIS
Sbjct: 121 FTVLTVQAHYPGLRPAACEMGSSGEGDECEEAKGYKALMFFSALYLVALGSGCLKPNIIS 180

Query: 181 HGADQFPKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAML 240
           HGADQF KQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVW+QTHSGMDLGFGVSTAAML
Sbjct: 181 HGADQFQKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAML 240

Query: 241 LGLISLLSGTSSFRNSPPRGTIFTPIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLH 300
           L LISLLSG S FRN+PPRG+IFTPIAQVFVAAFTKRKQICPSNSEMLHGS NGVVKLLH
Sbjct: 241 LALISLLSGASFFRNNPPRGSIFTPIAQVFVAAFTKRKQICPSNSEMLHGSHNGVVKLLH 300

Query: 301 TDKFRRESGKGRKSMETLHSNTSGTSENNSISDPHICLHHNLQHHLSTAPNLFSPTRSCH 360
           TD FR       KS ET      GT E +      +     ++  LS  P +F+ T   +
Sbjct: 301 TDNFRFLDKACIKSEET----RGGTREESPWKLCTVTQVEQVKIILSVIP-IFACTIIFN 360

Query: 361 EHMA-------------HQLLPN----PPASLQSIPYLILIFLVPLYETVFVPLARRLTG 420
             +A             +  L N    PPASLQSIPYLILIFLVPLYET FVPLARRLT 
Sbjct: 361 TILAQLQTFSVQQGASMNTTLTNSFQIPPASLQSIPYLILIFLVPLYETAFVPLARRLTA 420

Query: 421 TDSGISPLQRVGTGLFVATFSMVSAALVEQKRRNSSAAGSSLSILWIAPQFVIFGISELF 480
           TDSGI+PLQRVGTGLFVATFSMVSAALVEQKRR+S    S+LSILWIAPQFVIFGISELF
Sbjct: 421 TDSGITPLQRVGTGLFVATFSMVSAALVEQKRRHS----SNLSILWIAPQFVIFGISELF 480

Query: 481 TAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHND 540
           TAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVS+VNK++ GGSGGGWLSHND
Sbjct: 481 TAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSVVNKVSGGGSGGGWLSHND 540

Query: 541 LNNDRLDLFYWLLAGLSLVNFFNYLFWANRYSQ 555
           LN DRLDLFYWLLAGLSLVNF NYLFWANRYSQ
Sbjct: 541 LNKDRLDLFYWLLAGLSLVNFINYLFWANRYSQ 558

BLAST of Lag0039352 vs. ExPASy TrEMBL
Match: A0A6J1E215 (protein NRT1/ PTR FAMILY 4.4-like OS=Momordica charantia OX=3673 GN=LOC111025710 PE=3 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 3.9e-230
Identity = 438/571 (76.71%), Postives = 469/571 (82.14%), Query Frame = 0

Query: 1   MGDVLEKTKSKCKEMNSMSEDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60
           MGDVLEKTKS   + NS    TSVDWRGR C+SN HGGM AAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDVLEKTKSSKLKENS----TSVDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFSEMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFG 120
           NNLITYVF++MHFPLSKSANVVTNFVGTVF LSLLGGF+SDSYLGSFRTML+FG VEL G
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSG 120

Query: 121 FSLLTVQAHYEGLRPAGCEMGRGEEECVEAKGYEALMLYAALYLVALGSGCLKPNIISHG 180
           F+LLTVQAHY GLRPA C+M      CVEA G EA M + ALYLVALGSGCLKPNIISHG
Sbjct: 121 FTLLTVQAHYPGLRPAPCDMAATSTPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHG 180

Query: 181 ADQFPKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLG 240
           ADQFPK+DP Q KKLSTFFNCAYFAFCTGEL ALTLLVWLQTH+GMD+GFGVSTAAMLLG
Sbjct: 181 ADQFPKEDPKQLKKLSTFFNCAYFAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLG 240

Query: 241 LISLLSGTSSFRNSPPRGTIFTPIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLHTD 300
           LI LLS TS +RN+PPRGTIFTPIAQVFVAAFTKRKQICPSNSEMLHG++NG++KLLHTD
Sbjct: 241 LICLLSATSLYRNTPPRGTIFTPIAQVFVAAFTKRKQICPSNSEMLHGTENGLLKLLHTD 300

Query: 301 KFR--------RESGKGRKSMETLHSNTSGTSENN---SISDPHIC--LHHNLQHHLSTA 360
           KFR         E  +GR+         +   +     S+     C  + + +   L T 
Sbjct: 301 KFRFLDKACIKSEDDEGREESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQT- 360

Query: 361 PNLFSPTRSCHEHMAHQLLPN---PPASLQSIPYLILIFLVPLYETVFVPLARRLTGTDS 420
              FS  +     M   L P    PPASLQ+IPYLILIFLVPLYET FVPLARRLTG  S
Sbjct: 361 ---FSVQQG--TSMDSWLTPKFQIPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPS 420

Query: 421 GISPLQRVGTGLFVATFSMVSAALVEQKRRNSSAAGS-SLSILWIAPQFVIFGISELFTA 480
           GISPLQRVGTGLFVATFSMVSAALVE KRRNSSAAG+  LSILWI+PQFVIFGISELFTA
Sbjct: 421 GISPLQRVGTGLFVATFSMVSAALVETKRRNSSAAGAPPLSILWISPQFVIFGISELFTA 480

Query: 481 VGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHNDLN 540
           VGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRG + GGWLSHNDLN
Sbjct: 481 VGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLN 540

Query: 541 NDRLDLFYWLLAGLSLVNFFNYLFWANRYSQ 555
           NDRLDLFYWLLAGLS VNFFNYLFWANRYSQ
Sbjct: 541 NDRLDLFYWLLAGLSFVNFFNYLFWANRYSQ 561

BLAST of Lag0039352 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 649.0 bits (1673), Expect = 3.4e-186
Identity = 356/556 (64.03%), Postives = 413/556 (74.28%), Query Frame = 0

Query: 20  EDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSA 79
           +D  VD+RGRPC  +KHGG  AA FVLG QAFEMMAIAAVGNNLITYVF+EMHFPLSKSA
Sbjct: 23  DDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSA 82

Query: 80  NVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFGFSLLTVQAHYEGLRPAGCE 139
           N+VTNF+GTVF LSLLGGFLSDSYLGSFRTML+FG +E+ GF LL+VQAH   LRP  C 
Sbjct: 83  NLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECN 142

Query: 140 MGRGEEECVEAKGYEALMLYAALYLVALGSGCLKPNIISHGADQFPKQDPTQFKKLSTFF 199
           M      CVEA GY+A  LY AL LVALGSGCLKPNIISHGA+QF ++D    +KLS+FF
Sbjct: 143 MKSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFF 202

Query: 200 NCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLGLISLLSGTSSFRNSPPRGT 259
           N AYFAF  G+LIALTLLVW+QTHSGMD+GFGVS A M  G+ISL++GTS +RN PP G+
Sbjct: 203 NAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGS 262

Query: 260 IFTPIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVK---LLHTDKFR-------RESGKG 319
           IFTPIAQVFVAA TKRKQICPSN  M+H     +V+   LLH++KFR       +  GK 
Sbjct: 263 IFTPIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHSNKFRFLDKACIKTQGKA 322

Query: 320 RKS------METLHSNTSGTSENNSISDPHIC--LHHNLQHHLSTAPNLFSPTRSCHEHM 379
            +S      +E +H          S+     C  + + +   L T       + + H   
Sbjct: 323 MESPWRLCTIEQVHQ----VKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITK 382

Query: 380 AHQLLPNPPASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGISPLQRVGTGLFVATF 439
             Q+   PPASLQ+IPY+ILIF VPLYET FVPLAR+LTG DSGISPLQR+GTGLF+ATF
Sbjct: 383 TFQI---PPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATF 442

Query: 440 SMVSAALVEQKRRNSSAAGS-SLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQS 499
           SMV+AALVE+KRR S    +  LSI WIAPQF+IFG+SE+FTAVGLVEFFYKQS + MQS
Sbjct: 443 SMVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQS 502

Query: 500 FLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGG---GWLSHNDLNNDRLDLFYWLLAGL 554
           FLTAMTYCSYSFGFYLSS+LVS VN++T     G   GWL  NDLN DRLD FYWLLA L
Sbjct: 503 FLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASL 562

BLAST of Lag0039352 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 597.4 bits (1539), Expect = 1.2e-170
Identity = 330/549 (60.11%), Postives = 401/549 (73.04%), Query Frame = 0

Query: 18  MSEDTSVDWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSK 77
           ++E+ SVDWRGRP N NKHGGM AA FVLGLQAFE+M IAAVGNNLITYV +EMHFPLSK
Sbjct: 24  LAEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSK 83

Query: 78  SANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFGFSLLTVQAHYEGLRPAG 137
           +AN+VTNFVGT+F  +LLGG+LSD++LGSF T++IFGFVEL GF LL+VQAH   L+P  
Sbjct: 84  AANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPK 143

Query: 138 CEMGRGEEECVEAKGYEALMLYAALYLVALGSGCLKPNIISHGADQFPKQDPTQFKKLST 197
           C     ++ C EAKG++A++ + ALYLVALGSGC+KPN+I+HGADQF +  P Q K+LS+
Sbjct: 144 CN-PLIDQTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSS 203

Query: 198 FFNCAYFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLGLISLLSGTSSFRNSPPR 257
           +FN AYFAF  GELIALTLLVW+QTHSGMD+GFGVS AAM +G+ISL+SGT  FRN  PR
Sbjct: 204 YFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPR 263

Query: 258 GTIFTPIAQVFVAAFTKRKQICPSNSEMLHGSQ---NGVV---KLLHTDKFR-------- 317
            +IFTPIA V VAA  KRK   PS+  MLHG     N VV    L HT +FR        
Sbjct: 264 RSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIK 323

Query: 318 RESGKGRKSMETLHSNTSGTSENNSISDPHICLHHNLQHHLSTAPNLFSPTR-SCHEHMA 377
            +    ++S   L + T        IS   I     + + +      FS  + S      
Sbjct: 324 IQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRL 383

Query: 378 HQLLPNPPASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGISPLQRVGTGLFVATFS 437
                 PPASLQ+IPY++LIFLVPLY++  VP AR+LTG +SGI PL R+G GLF++TFS
Sbjct: 384 SNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFS 443

Query: 438 MVSAALVEQKRRNSSAA-GSSLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSF 497
           MVSAA++E+KRR+SS   G  LSI WI PQF+IFGISE+FTAVGL+EFFYKQS +GM+SF
Sbjct: 444 MVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESF 503

Query: 498 LTAMTYCSYSFGFYLSSLLVSLVNKITRGG-SGGGWLSHNDLNNDRLDLFYWLLAGLSLV 550
           L A+TYCSYSFGFY SS+LVS+VNKIT       GWL  NDLN DRLDLFYWLLA LSL+
Sbjct: 504 LMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLL 563

BLAST of Lag0039352 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 333.2 bits (853), Expect = 4.2e-91
Identity = 212/577 (36.74%), Postives = 307/577 (53.21%), Query Frame = 0

Query: 25  DWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVTN 84
           DWR R     +HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH   SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 85  FVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFGFSLLTVQAHYEGLRPAGCEMGRGE 144
           F+GT F L+LLGGFLSD++  +F+  LI   +E  G  +LT+QA    L P  C+     
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCD----S 134

Query: 145 EECVEAKGYEALMLYAALYLVALGSGCLKPNIISHGADQFPKQDPTQFKKLSTFFNCAYF 204
             C E  G +A ML+  LYLVALG G +K ++ SHGA+QF +  P   K+ STFFN  YF
Sbjct: 135 PTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFN--YF 194

Query: 205 AFC--TGELIALTLLVWLQTHSGMDLGFGVSTAAMLLGLISLLSGTSSFRNSPPRGTIFT 264
            FC   G L+A+T +VWL+ + G + GFGVST A+ + ++  LSG+  +RN  P G+  T
Sbjct: 195 VFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLT 254

Query: 265 PIAQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLHTDKFRRESGKGRKSMETLHSNTSG 324
            I +V +AA  K    C S+     GS +  V  +          KG+K +E+       
Sbjct: 255 TILKVLLAASVK----CCSS-----GSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKP 314

Query: 325 TSEN---------------NSISDP---HICLHHNLQH------HLSTAP---------- 384
             E                N  +D    H  L   +Q        L   P          
Sbjct: 315 RQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNC 374

Query: 385 -----NLFSPTRSCHEHMAHQLLPNPPASLQSIPYLILIFLVPLYETVFVPLARRLTGTD 444
                + FS  ++   +     L  PPASL   P + ++ L P+Y+ + +P AR+ T T+
Sbjct: 375 CLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTE 434

Query: 445 SGISPLQRVGTGLFVATFSMVSAALVEQKRRN--------SSAAGSSLSILWIAPQFVIF 504
           +G++ LQR+G GL ++  +M  AALVE KR+          S     ++ LWIA Q++  
Sbjct: 435 TGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFL 494

Query: 505 GISELFTAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGG 553
           G ++LFT  GL+E+F+ ++   M+S  T++++ S + G+YLSS++VS+VN IT       
Sbjct: 495 GSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTP 554

BLAST of Lag0039352 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 313.5 bits (802), Expect = 3.4e-85
Identity = 197/550 (35.82%), Postives = 304/550 (55.27%), Query Frame = 0

Query: 25  DWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVTN 84
           DWR +     +HGGM AA+FVL ++  E +A  A  +NL+ Y+ + MH  L++S++ VT 
Sbjct: 13  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 72

Query: 85  FVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFGFSLLTVQAHYEGLRPAGCEMGRGE 144
           F+ T F L+LLGGFL+D++  +F   LI   +E  G  LLT+QA    L P  C+     
Sbjct: 73  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCK-SSAA 132

Query: 145 EECVEAKGYEALMLYAALYLVALGSGCLKPNIISHGADQFPKQDPTQFKKLSTFFNCAYF 204
             C    G +A  L+  LYLV+LG G +K ++ SHGA+QF +  P   K+ STFFN   F
Sbjct: 133 LRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVF 192

Query: 205 AFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLGLISLLSGTSSFRNSPPRGTIFTPI 264
               G L+A+T +VW++ + G + GFGVST ++ L ++  L G+  ++N  PRG+  T I
Sbjct: 193 CLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTI 252

Query: 265 AQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLHTDKFRRESGKGRKSMETLHSNTSGTS 324
            +V +AA       C S +   H +    V+  H +K   +S     S+  L+    G +
Sbjct: 253 FKVLLAASIVS---CSSKTSSNHFTSR-EVQSEHEEKTPSQS--LTNSLTCLNKAIEGKT 312

Query: 325 EN-------NSISDPHICLHHNLQHHLSTAPNLFSPTRSCHE-HMA---HQLLPN---PP 384
            +         + D  I L        +   N      S +  H A   ++ + N   P 
Sbjct: 313 HHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPS 372

Query: 385 ASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGISPLQRVGTGLFVATFSMVSAALVE 444
           ASL   P + ++ L P Y+ + +P AR++T ++ GI+ LQR+G GL ++  +M  AALVE
Sbjct: 373 ASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVE 432

Query: 445 QKRRN--------SSAAGSSLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFL 504
            KR+          S     ++ LWIA Q++  G ++LFT  GL+EFF+ ++   M+S  
Sbjct: 433 LKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLA 492

Query: 505 TAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHNDLNNDRLDLFYWLLAGLSLVNF 553
           T++++ S + G+YLSS++V +VN++T+      WL    LN +RLDLFYWL+  LS+VNF
Sbjct: 493 TSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGEK-LNRNRLDLFYWLMCVLSVVNF 552

BLAST of Lag0039352 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 313.5 bits (802), Expect = 3.4e-85
Identity = 197/550 (35.82%), Postives = 304/550 (55.27%), Query Frame = 0

Query: 25  DWRGRPCNSNKHGGMTAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVTN 84
           DWR +     +HGGM AA+FVL ++  E +A  A  +NL+ Y+ + MH  L++S++ VT 
Sbjct: 17  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 76

Query: 85  FVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFVELFGFSLLTVQAHYEGLRPAGCEMGRGE 144
           F+ T F L+LLGGFL+D++  +F   LI   +E  G  LLT+QA    L P  C+     
Sbjct: 77  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCK-SSAA 136

Query: 145 EECVEAKGYEALMLYAALYLVALGSGCLKPNIISHGADQFPKQDPTQFKKLSTFFNCAYF 204
             C    G +A  L+  LYLV+LG G +K ++ SHGA+QF +  P   K+ STFFN   F
Sbjct: 137 LRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVF 196

Query: 205 AFCTGELIALTLLVWLQTHSGMDLGFGVSTAAMLLGLISLLSGTSSFRNSPPRGTIFTPI 264
               G L+A+T +VW++ + G + GFGVST ++ L ++  L G+  ++N  PRG+  T I
Sbjct: 197 CLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTI 256

Query: 265 AQVFVAAFTKRKQICPSNSEMLHGSQNGVVKLLHTDKFRRESGKGRKSMETLHSNTSGTS 324
            +V +AA       C S +   H +    V+  H +K   +S     S+  L+    G +
Sbjct: 257 FKVLLAASIVS---CSSKTSSNHFTSR-EVQSEHEEKTPSQS--LTNSLTCLNKAIEGKT 316

Query: 325 EN-------NSISDPHICLHHNLQHHLSTAPNLFSPTRSCHE-HMA---HQLLPN---PP 384
            +         + D  I L        +   N      S +  H A   ++ + N   P 
Sbjct: 317 HHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPS 376

Query: 385 ASLQSIPYLILIFLVPLYETVFVPLARRLTGTDSGISPLQRVGTGLFVATFSMVSAALVE 444
           ASL   P + ++ L P Y+ + +P AR++T ++ GI+ LQR+G GL ++  +M  AALVE
Sbjct: 377 ASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVE 436

Query: 445 QKRRN--------SSAAGSSLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFL 504
            KR+          S     ++ LWIA Q++  G ++LFT  GL+EFF+ ++   M+S  
Sbjct: 437 LKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLA 496

Query: 505 TAMTYCSYSFGFYLSSLLVSLVNKITRGGSGGGWLSHNDLNNDRLDLFYWLLAGLSLVNF 553
           T++++ S + G+YLSS++V +VN++T+      WL    LN +RLDLFYWL+  LS+VNF
Sbjct: 497 TSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGEK-LNRNRLDLFYWLMCVLSVVNF 556