Lag0039336 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0039336
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	22.0 kDa class IV heat shock protein
Location	chr2: 41607038 .. 41607592 (+)
RNA-Seq Expression	Lag0039336
Synteny	Lag0039336

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAAATCCAAAGCAGCGAACCTCCTCTCGTGCGCAATTTTCATCTTCACTCTTCATTTCATCTCATCAAAAGCCTCTCTTCTTCCACTCCTCGATCCCTTCGCGATTCTCGAACAAACCCCATTCGGCTTCGGCAAGGAAGACAGAGATGCTCTGCAGCTGCAGCCGTTCCCACCGGCGAGAGTCGACTGGAAAGAGACGCCGGAGAGCCACGTCATCATTCTGGACGTGCCTGGGCTGAACAAGGACGAGCTGAAGATCGAGTTGGACGACGAGAACAGGATCCTCAAAGTGATCGGAGAGAGGAAACGGGAAGAGGAAGAACAGAGCGATCACTGGCACCGCTTGGAACGGTCTTATGGCAAATTCTGGAGGCAGTTTCGGGTGCCCGTTAATGTGGATTTGGAGTCTGTTACAGCTCAGCTTCGTAATGGGGTTCTTACGGTGACTCTGTCGAAGGTCTCGCCGGAGAAGATCAAGGGTCCGAGAGTTGTCAGCATTTCGGGCGGAGATGATCAAGTCAGGTCCGGCGAGGCCAAGCAGGAGCTGTGA

mRNA sequence

ATGGCAAAATCCAAAGCAGCGAACCTCCTCTCGTGCGCAATTTTCATCTTCACTCTTCATTTCATCTCATCAAAAGCCTCTCTTCTTCCACTCCTCGATCCCTTCGCGATTCTCGAACAAACCCCATTCGGCTTCGGCAAGGAAGACAGAGATGCTCTGCAGCTGCAGCCGTTCCCACCGGCGAGAGTCGACTGGAAAGAGACGCCGGAGAGCCACGTCATCATTCTGGACGTGCCTGGGCTGAACAAGGACGAGCTGAAGATCGAGTTGGACGACGAGAACAGGATCCTCAAAGTGATCGGAGAGAGGAAACGGGAAGAGGAAGAACAGAGCGATCACTGGCACCGCTTGGAACGGTCTTATGGCAAATTCTGGAGGCAGTTTCGGGTGCCCGTTAATGTGGATTTGGAGTCTGTTACAGCTCAGCTTCGTAATGGGGTTCTTACGGTGACTCTGTCGAAGGTCTCGCCGGAGAAGATCAAGGGTCCGAGAGTTGTCAGCATTTCGGGCGGAGATGATCAAGTCAGGTCCGGCGAGGCCAAGCAGGAGCTGTGA

Coding sequence (CDS)

ATGGCAAAATCCAAAGCAGCGAACCTCCTCTCGTGCGCAATTTTCATCTTCACTCTTCATTTCATCTCATCAAAAGCCTCTCTTCTTCCACTCCTCGATCCCTTCGCGATTCTCGAACAAACCCCATTCGGCTTCGGCAAGGAAGACAGAGATGCTCTGCAGCTGCAGCCGTTCCCACCGGCGAGAGTCGACTGGAAAGAGACGCCGGAGAGCCACGTCATCATTCTGGACGTGCCTGGGCTGAACAAGGACGAGCTGAAGATCGAGTTGGACGACGAGAACAGGATCCTCAAAGTGATCGGAGAGAGGAAACGGGAAGAGGAAGAACAGAGCGATCACTGGCACCGCTTGGAACGGTCTTATGGCAAATTCTGGAGGCAGTTTCGGGTGCCCGTTAATGTGGATTTGGAGTCTGTTACAGCTCAGCTTCGTAATGGGGTTCTTACGGTGACTCTGTCGAAGGTCTCGCCGGAGAAGATCAAGGGTCCGAGAGTTGTCAGCATTTCGGGCGGAGATGATCAAGTCAGGTCCGGCGAGGCCAAGCAGGAGCTGTGA

Protein sequence

MAKSKAANLLSCAIFIFTLHFISSKASLLPLLDPFAILEQTPFGFGKEDRDALQLQPFPPARVDWKETPESHVIILDVPGLNKDELKIELDDENRILKVIGERKREEEEQSDHWHRLERSYGKFWRQFRVPVNVDLESVTAQLRNGVLTVTLSKVSPEKIKGPRVVSISGGDDQVRSGEAKQEL

Homology

[Close]

BLAST of Lag0039336 vs. NCBI nr
Match: KAA0039359.1 (22.0 kDa class IV heat shock protein [Cucumis melo var. makuwa] >TYK00542.1 22.0 kDa class IV heat shock protein [Cucumis melo var. makuwa])

HSP 1 Score: 256.1 bits (653), Expect = 2.2e-64
Identity = 132/189 (69.84%), Postives = 151/189 (79.89%), Query Frame = 0


Query: 1   MAKSKAANLLSCAIFIFTLHFISSKASLLPLLDPFAILEQTPFG-FGKEDRDALQLQPFP 60
           MA+SKA  L+   +FIF     SS+ SLLP +DPF ILEQTPFG    E++DALQ QP P
Sbjct: 1   MAESKAIQLIFSTVFIFAFLITSSQPSLLPFIDPFGILEQTPFGLLENENKDALQQQPLP 60

Query: 61  PARVDWKETPESHVIILDVPGLNKDELKIELDDENRILKVIGERKREEEEQSDHWHRLER 120
           PARVDWKET ESH I++DVPG+NK+ELKIELD+ENR+LKVIGERKREEE+QSDHWHRLER
Sbjct: 61  PARVDWKETAESHEIMVDVPGMNKEELKIELDEENRVLKVIGERKREEEKQSDHWHRLER 120

Query: 121 SYGKFWRQFRVPVNVDLESVTAQLRNGVLTVTLSKVSPEKIKGPRVVSISGGDDQV---- 180
           SYGKFWRQ R+PVN DLESV A+L NGVL +TLSK+SPEKIKGPRVV I  G        
Sbjct: 121 SYGKFWRQLRLPVNADLESVKAKLENGVLKITLSKLSPEKIKGPRVVGILDGQTPAGEIN 180

Query: 181 RSGEAKQEL 185
           +SG AKQEL
Sbjct: 181 KSGAAKQEL 189

[Close]

BLAST of Lag0039336 vs. NCBI nr
Match: XP_008459498.1 (PREDICTED: 22.0 kDa class IV heat shock protein [Cucumis melo])

HSP 1 Score: 253.4 bits (646), Expect = 1.4e-63
Identity = 133/191 (69.63%), Postives = 153/191 (80.10%), Query Frame = 0


Query: 1   MAKSKAANLLSCAIFIFTLHFISSKASLLPLLDPFAILEQTPFG-FGKEDRDALQLQPFP 60
           MA+SKA  L+   +FIF     SS+ SLLP +DPF ILEQTPFG    E++DALQ QP P
Sbjct: 1   MAESKAIQLIFSTVFIFAFLITSSQPSLLPFIDPFGILEQTPFGLLENENKDALQQQPLP 60

Query: 61  PARVDWKETPESHVIILDVPGLNKDELKIELDDENRILKVIGERKREEEEQSDHWHRLER 120
           PARVDWKET ESH I++DVPG+NK+ELKIELD+ENR+LKVIGERKREEE+QSDHWHRLER
Sbjct: 61  PARVDWKETAESHEIMVDVPGMNKEELKIELDEENRVLKVIGERKREEEKQSDHWHRLER 120

Query: 121 SYGKFWRQFRVPVNVDLESVTAQLRNGVLTVTLSKVSPEKIKGPRVVSISGGDDQV---- 180
           SYGKFWRQ R+PVN DLESV A+L NGVL +TLSK+SPEKIKGPRVV I   D+Q     
Sbjct: 121 SYGKFWRQLRLPVNADLESVKAKLENGVLKITLSKLSPEKIKGPRVVGIL--DEQPPAGE 180

Query: 181 --RSGEAKQEL 185
             +SG AKQEL
Sbjct: 181 INKSGAAKQEL 189

[Close]

BLAST of Lag0039336 vs. NCBI nr
Match: ADU55793.1 (HSP20.3 [Citrullus lanatus])

HSP 1 Score: 246.1 bits (627), Expect = 2.3e-61
Identity = 130/179 (72.63%), Postives = 147/179 (82.12%), Query Frame = 0


Query: 1   MAKSKAANLLSCAIFIFTLHFISSKASLLPLLDPFAILEQTPFG-FGKEDRDALQLQPFP 60
           MA  KA  L   AIFI +     S+ SLLP +DPF ILEQTPFG    E+RDALQ QP P
Sbjct: 1   MANFKAVQLFLFAIFISSFLLTPSEPSLLPFIDPFGILEQTPFGLLENENRDALQ-QPLP 60

Query: 61  PARVDWKETPESHVIILDVPGLNKDELKIELDDENRILKVIGERKREEEEQSDHWHRLER 120
           PARVDWKETPESHVI+LDVPG+NK+E+KIELD+ENRILKVIGERKREEE+QS+HWHRLER
Sbjct: 61  PARVDWKETPESHVIMLDVPGMNKEEMKIELDEENRILKVIGERKREEEKQSEHWHRLER 120

Query: 121 SYGKFWRQFRVPVNVDLESVTAQLRNGVLTVTLSKVSPEKIKGPRVVSISGGDDQVRSG 179
           SYGKFWRQFR+P N D+ESV AQL+NGVL VTLSK+SPEKIKGPRVV I   D+Q  +G
Sbjct: 121 SYGKFWRQFRLPSNADMESVKAQLQNGVLKVTLSKLSPEKIKGPRVVGIL--DEQPPAG 176

[Close]

BLAST of Lag0039336 vs. NCBI nr
Match: XP_004141518.1 (22.0 kDa class IV heat shock protein [Cucumis sativus])

HSP 1 Score: 245.0 bits (624), Expect = 5.2e-61
Identity = 128/189 (67.72%), Postives = 147/189 (77.78%), Query Frame = 0


Query: 1   MAKSKAANLLSCAIFIFTLHFISSKASLLPLLDPFAILEQTPFG-FGKEDRDALQLQPFP 60
           MA+SKA  L+   IFIF     SS  SLLP +DPF ILEQTPFG    E++D LQ QP P
Sbjct: 1   MAESKAIQLIFSTIFIFAFLLTSSHPSLLPFIDPFGILEQTPFGLLENENKDVLQQQPLP 60

Query: 61  PARVDWKETPESHVIILDVPGLNKDELKIELDDENRILKVIGERKREEEEQSDHWHRLER 120
           PARVDWKET ESH I++DVPG+NK+ELKIELD+ENR+LKVIGERKREEE+QSDHWHRLER
Sbjct: 61  PARVDWKETAESHEIMVDVPGMNKEELKIELDEENRVLKVIGERKREEEKQSDHWHRLER 120

Query: 121 SYGKFWRQFRVPVNVDLESVTAQLRNGVLTVTLSKVSPEKIKGPRVVSI----SGGDDQV 180
           SYGKFWRQ R+PVN DLESV A+L NGVL ++L K+S EKIKGPRVV I        +  
Sbjct: 121 SYGKFWRQLRLPVNADLESVKAKLENGVLKISLLKLSQEKIKGPRVVGILDEQPPAGEMN 180

Query: 181 RSGEAKQEL 185
           +SG AKQEL
Sbjct: 181 KSGAAKQEL 189

[Close]

BLAST of Lag0039336 vs. NCBI nr
Match: KAE8648973.1 (hypothetical protein Csa_008304 [Cucumis sativus])

HSP 1 Score: 245.0 bits (624), Expect = 5.2e-61
Identity = 128/189 (67.72%), Postives = 147/189 (77.78%), Query Frame = 0


Query: 1   MAKSKAANLLSCAIFIFTLHFISSKASLLPLLDPFAILEQTPFG-FGKEDRDALQLQPFP 60
           MA+SKA  L+   IFIF     SS  SLLP +DPF ILEQTPFG    E++D LQ QP P
Sbjct: 558 MAESKAIQLIFSTIFIFAFLLTSSHPSLLPFIDPFGILEQTPFGLLENENKDVLQQQPLP 617

Query: 61  PARVDWKETPESHVIILDVPGLNKDELKIELDDENRILKVIGERKREEEEQSDHWHRLER 120
           PARVDWKET ESH I++DVPG+NK+ELKIELD+ENR+LKVIGERKREEE+QSDHWHRLER
Sbjct: 618 PARVDWKETAESHEIMVDVPGMNKEELKIELDEENRVLKVIGERKREEEKQSDHWHRLER 677

Query: 121 SYGKFWRQFRVPVNVDLESVTAQLRNGVLTVTLSKVSPEKIKGPRVVSI----SGGDDQV 180
           SYGKFWRQ R+PVN DLESV A+L NGVL ++L K+S EKIKGPRVV I        +  
Sbjct: 678 SYGKFWRQLRLPVNADLESVKAKLENGVLKISLLKLSQEKIKGPRVVGILDEQPPAGEMN 737

Query: 181 RSGEAKQEL 185
           +SG AKQEL
Sbjct: 738 KSGAAKQEL 746

[Close]

BLAST of Lag0039336 vs. ExPASy Swiss-Prot
Match: P30236 (22.0 kDa class IV heat shock protein OS=Glycine max OX=3847 GN=HSP22.0 PE=3 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.0e-48
Identity = 104/190 (54.74%), Postives = 131/190 (68.95%), Query Frame = 0


Query: 14  IFIFTLHFISSKASLLPLL----------------DPFAILEQTPFGFGKEDRDALQLQP 73
           +F   L    +  SLLP +                DPF +LE  PFG  K++        
Sbjct: 8   LFFLLLCVAKANGSLLPFMDPPITLLADLWSDRFPDPFRVLEHIPFGVDKDEAS----MA 67

Query: 74  FPPARVDWKETPESHVIILDVPGLNKDELKIELDDENRILKVIGERKREEEEQSDHWHRL 133
             PARVDWKETPE HVI+LDVPGL ++E+K+E+ +ENR+L+V GERK+EEE++ DHWHR+
Sbjct: 68  MSPARVDWKETPEGHVIMLDVPGLKREEIKVEV-EENRVLRVSGERKKEEEKKGDHWHRV 127

Query: 134 ERSYGKFWRQFRVPVNVDLESVTAQLRNGVLTVTLSKVSPEKIKGPRVVSISGGDDQ--- 185
           ERSYGKFWRQFR+P NVDL+SV A+L NGVLT+TL K+SP KIKGPRVVSI+G D Q   
Sbjct: 128 ERSYGKFWRQFRLPQNVDLDSVKAKLENGVLTLTLDKLSPGKIKGPRVVSIAGEDHQQGN 187

[Close]

BLAST of Lag0039336 vs. ExPASy Swiss-Prot
Match: Q38806 (22.0 kDa heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP22.0 PE=2 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 1.8e-45
Identity = 92/153 (60.13%), Postives = 119/153 (77.78%), Query Frame = 0


Query: 33  DPFAILEQTPFGFGKEDRDALQLQPFPPARVDWKETPESHVIILDVPGLNKDELKIELDD 92
           DPF ILE+ P G  ++   AL      PARVDWKET E H I+LD+PGL KDE+KIE+ +
Sbjct: 48  DPFKILERIPLGLERDTSVALS-----PARVDWKETAEGHEIMLDIPGLKKDEVKIEV-E 107

Query: 93  ENRILKVIGERKREEEEQSDHWHRLERSYGKFWRQFRVPVNVDLESVTAQLRNGVLTVTL 152
           EN +L+V GERKREEE++ D WHR+ERSYGKFWRQF++P NVD+ESV A+L NGVLT+ L
Sbjct: 108 ENGVLRVSGERKREEEKKGDQWHRVERSYGKFWRQFKLPDNVDMESVKAKLENGVLTINL 167

Query: 153 SKVSPEKIKGPRVVSISGGDDQ---VRSGEAKQ 183
           +K+SPEK+KGPRVV+I+  +DQ   + S E+K+
Sbjct: 168 TKLSPEKVKGPRVVNIAAEEDQTAKISSSESKE 194

[Close]

BLAST of Lag0039336 vs. ExPASy Swiss-Prot
Match: P19244 (22.7 kDa class IV heat shock protein OS=Pisum sativum OX=3888 GN=HSP22.7 PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 9.2e-45
Identity = 93/169 (55.03%), Postives = 122/169 (72.19%), Query Frame = 0


Query: 22  ISSKASLLPLL----------------DPFAILEQTPFGFGKEDRDALQLQPFPPARVDW 81
           + +KASLLP +                DPF +LEQ P+G  K +           ARVDW
Sbjct: 24  LKAKASLLPFIDSPNTLLSDLWSDRFPDPFRVLEQIPYGVEKHEPSI----TLSHARVDW 83

Query: 82  KETPESHVIILDVPGLNKDELKIELDDENRILKVIGERKREEEEQSDHWHRLERSYGKFW 141
           KETPE HVI++DVPGL KD++KIE+ +ENR+L+V GERK+EE+++ DHWHR+ERSYGKFW
Sbjct: 84  KETPEGHVIMVDVPGLKKDDIKIEV-EENRVLRVSGERKKEEDKKGDHWHRVERSYGKFW 143

Query: 142 RQFRVPVNVDLESVTAQLRNGVLTVTLSKVSPEKIKGPRVVSISGGDDQ 175
           RQF++P NVDL+SV A++ NGVLT+TL K+S +KIKGPR+VSI   DD+
Sbjct: 144 RQFKLPQNVDLDSVKAKMENGVLTLTLHKLSHDKIKGPRMVSIVEEDDK 187

[Close]

BLAST of Lag0039336 vs. ExPASy Swiss-Prot
Match: Q7XUW5 (23.2 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP23.2 PE=2 SV=2)

HSP 1 Score: 164.1 bits (414), Expect = 1.5e-39
Identity = 81/152 (53.29%), Postives = 111/152 (73.03%), Query Frame = 0


Query: 28  LLPLLDPFAILEQTPFGFGKEDRDALQLQPFPPARVDWKETPESHVIILDVPGLNKDELK 87
           LL   DPF ILE  PFGF ++D   L +     ARVDW+ET ++H +++DVPG+ K++L+
Sbjct: 48  LLAAADPFRILEHVPFGFDRDDVAMLSM-----ARVDWRETGDAHEVVVDVPGMRKEDLR 107

Query: 88  IELDDENRILKVIGERKREEEEQ----SDHWHRLERSYGKFWRQFRVPVNVDLESVTAQL 147
           +E++D NR+L++ GER+REE  +     DHWHR ERSYG+FWRQ R+P N DL+S+ A L
Sbjct: 108 VEVED-NRVLRISGERRREETTEQKGGGDHWHREERSYGRFWRQLRLPDNADLDSIAASL 167

Query: 148 RNGVLTVTLSKVSPEKIKGPRVVSI--SGGDD 174
            NGVLTV   K++P++IKGPRVV I  +GGDD
Sbjct: 168 DNGVLTVRFRKLAPDQIKGPRVVGIASAGGDD 193

[Close]

BLAST of Lag0039336 vs. ExPASy Swiss-Prot
Match: Q84J50 (17.7 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP17.7 PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.9e-29
Identity = 74/149 (49.66%), Postives = 95/149 (63.76%), Query Frame = 0


Query: 33  DPFAI-----LEQTPFGFGKEDRDALQLQP--------FPPARVDWKETPESHVIILDVP 92
           DPF++     ++  PFG G     +  L P        F  AR+DWKETPE HV   DVP
Sbjct: 11  DPFSLDLWDPVDGFPFGSGGSSSSSGSLFPRANSDAAAFAGARIDWKETPEVHVFKADVP 70

Query: 93  GLNKDELKIELDDENRILKVIGERKREEEEQSDHWHRLERSYGKFWRQFRVPVNVDLESV 152
           GL K+E+K+E+DD N IL++ GER RE+EE+SD WHR+ERS GKF R+FR+P N   E +
Sbjct: 71  GLKKEEVKVEVDDGN-ILQISGERSREQEEKSDKWHRVERSSGKFLRRFRLPENTKPEQI 130

Query: 153 TAQLRNGVLTVTLSKVSPEKIKGPRVVSI 169
            A + NGVLTVT+ K  P+K   P V SI
Sbjct: 131 KASMENGVLTVTVPKEEPKK---PDVKSI 155

The following BLAST results are available for this feature:

BLAST of Lag0039336 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0039359.1	2.2e-64	69.84	22.0 kDa class IV heat shock protein [Cucumis melo var. makuwa] >TYK00542.1 22.0...	[more]
XP_008459498.1	1.4e-63	69.63	PREDICTED: 22.0 kDa class IV heat shock protein [Cucumis melo]	[more]
ADU55793.1	2.3e-61	72.63	HSP20.3 [Citrullus lanatus]	[more]
XP_004141518.1	5.2e-61	67.72	22.0 kDa class IV heat shock protein [Cucumis sativus]	[more]
KAE8648973.1	5.2e-61	67.72	hypothetical protein Csa_008304 [Cucumis sativus]	[more]

BLAST of Lag0039336 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P30236	1.0e-48	54.74	22.0 kDa class IV heat shock protein OS=Glycine max OX=3847 GN=HSP22.0 PE=3 SV=1	[more]
Q38806	1.8e-45	60.13	22.0 kDa heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP22.0 PE=2 SV=1	[more]
P19244	9.2e-45	55.03	22.7 kDa class IV heat shock protein OS=Pisum sativum OX=3888 GN=HSP22.7 PE=2 SV...	[more]
Q7XUW5	1.5e-39	53.29	23.2 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP23.2 ...	[more]
Q84J50	1.9e-29	49.66	17.7 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 64..169 e-value: 7.0E-28 score: 96.8
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 52..171 score: 26.286541
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 31..170 e-value: 1.6E-36 score: 127.3
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 57..165
None	No IPR available	PANTHER	PTHR11527:SF267	22.0 KDA HEAT SHOCK PROTEIN	coord: 26..174
None	No IPR available	CDD	cd06472	ACD_ScHsp26_like	coord: 62..154 e-value: 3.42743E-46 score: 145.14
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 26..174
IPR007052	CS domain	PROSITE	PS51203	CS	coord: 58..166 score: 9.05401

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0039336.1	Lag0039336.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051259	protein complex oligomerization
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009651	response to salt stress
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0051082	unfolded protein binding