Lag0039317 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0039317 vs. NCBI nr
Match: XP_038891432.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 1188.7 bits (3074), Expect = 0.0e+00
Identity = 590/705 (83.69%), Postives = 631/705 (89.50%), Query Frame = 0

Query: 1   MGSHPKGKVSTPSHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEG 60
           MGSHPKGKVST SHHIRLL+ETIGSTF PHSLLHSY RSFNGFV KLTE EAKKVS+MEG
Sbjct: 37  MGSHPKGKVSTSSHHIRLLKETIGSTFAPHSLLHSYTRSFNGFVAKLTEPEAKKVSEMEG 96

Query: 61  VVSVFPNGIKQLHTTRSWDFMGFSEQVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGP 120
           V+SVFPNG KQLHTTRSWDFMGFSEQV RVP+VESN+IVGV DSGIWPESPSFD  GYGP
Sbjct: 97  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTGYGP 156

Query: 121 PPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLV 180
           PPP WKGSCEVSANFSCNNKIIG R+YRS G+HPE DI+GP DS+GHGTHTAS VAGG+V
Sbjct: 157 PPPKWKGSCEVSANFSCNNKIIGGRAYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGVV 216

Query: 181 RQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGAS 240
           RQ SM+GLG GTARGGVPSARIA YKVCWSDGC DADILAAFDDAIADGV+IISGSLG S
Sbjct: 217 RQASMLGLGLGTARGGVPSARIASYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGS 276

Query: 241 RVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTK 300
            + DYFNDSIAIGSFHAMKKGILTSLAVGNNGPD T+IVNF+PWSLSVAASTTDRKF TK
Sbjct: 277 GIRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETK 336

Query: 301 VELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIV 360
           VELGDGR F+GVSINTFDL G QIPLV+AGDIPKAPFD SVSRMCFE TVE ELVKGKIV
Sbjct: 337 VELGDGREFNGVSINTFDLKGKQIPLVYAGDIPKAPFDSSVSRMCFENTVELELVKGKIV 396

Query: 361 VCDSLAVPEGVVAVEGAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINSTNSVA 420
           VCDSLAVP GVVA++GAAGIIMQDDSPQDDTNS+P+PASHL PK GALILSYINSTNS+ 
Sbjct: 397 VCDSLAVPGGVVAIKGAAGIIMQDDSPQDDTNSFPIPASHLAPKPGALILSYINSTNSLP 456

Query: 421 TATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAKD 480
           TATI+KSTERKRKRAP                   PDL+GPGVEILAAWPS  SPSGA++
Sbjct: 457 TATIMKSTERKRKRAPFVASFSSRGPNPITPNILKPDLTGPGVEILAAWPSIASPSGAEE 516

Query: 481 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRNPAGEF 540
           D+KRV YNI+SGTSMACPHVTAAAAYVKSFHPTWSPAALKSAL+TTAF MSLKRNP  EF
Sbjct: 517 DSKRVAYNILSGTSMACPHVTAAAAYVKSFHPTWSPAALKSALITTAFSMSLKRNPDKEF 576

Query: 541 AYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVFD 600
            YGAGHINPLGAV PGL+YNASEIDY+KFLCGQGY TELLQRVSEDN TCS  NSDTVFD
Sbjct: 577 GYGAGHINPLGAVQPGLVYNASEIDYVKFLCGQGYNTELLQRVSEDNDTCSSNNSDTVFD 636

Query: 601 LNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVNPSKNLEITVKPSVLSFKNLG 660
           LNYPSFALST  SK INQ+Y+R VTNVGSKYATYKATI+NP KNLEITV PSVLSFKNLG
Sbjct: 637 LNYPSFALSTNISKSINQVYKRIVTNVGSKYATYKATIINPWKNLEITVNPSVLSFKNLG 696

Query: 661 EEQSFELTIKGRIGKNIESASLVWDDGRHKVKSPITVFDANIHST 688
           EEQ+F LTIKGRI KNIESASLVW+DG+HKV+SPIT+FD+NIHS+
Sbjct: 697 EEQTFVLTIKGRISKNIESASLVWEDGKHKVRSPITIFDSNIHSS 741

BLAST of Lag0039317 vs. NCBI nr
Match: XP_016900177.1 (PREDICTED: cucumisin-like isoform X1 [Cucumis melo] >KAA0034649.1 cucumisin-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1158.3 bits (2995), Expect = 0.0e+00
Identity = 578/705 (81.99%), Postives = 623/705 (88.37%), Query Frame = 0

Query: 1   MGSHPKGKVSTPSHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEG 60
           MGSHPKGKVST SHHIRLL+ETIGS+F PHSLLHS++RSFNGFV KLTEAEAKKVS+MEG
Sbjct: 37  MGSHPKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEAKKVSEMEG 96

Query: 61  VVSVFPNGIKQLHTTRSWDFMGFSEQVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGP 120
           V+SVFPNG KQLHTTRSWDFMGFSEQV RVP+VESN+IVGV DSGIWPESPSFD  GYGP
Sbjct: 97  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTGYGP 156

Query: 121 PPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLV 180
           PP  WKGSCEVSANFSCNNKIIGARSYRS G++PE DI+GP DS+GHGTHTAS VAGGLV
Sbjct: 157 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 216

Query: 181 RQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGAS 240
           RQ +M+GLG GTARGGVPSARIA YKVCWSDGC DADILAAFDDAIADGV+IISGSLG S
Sbjct: 217 RQANMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 276

Query: 241 RVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTK 300
              DYFNDSIAIGSFHAMKKGILTSLAVGNNGPD TTIVNF+PWSLSVAASTTDRKF TK
Sbjct: 277 GFRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 336

Query: 301 VELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIV 360
           VELGDGR F+GVS+NTFD+ G QIPLV+AGDIPKAPFD SVSR+CFE T++ +LVKGKIV
Sbjct: 337 VELGDGREFNGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTIDLKLVKGKIV 396

Query: 361 VCDSLAVPEGVVAVEGAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINSTNSVA 420
           VCDSL VP GV AV+GA GIIMQDDS  DDTNS+P+PASHL P+ GALILSYINSTNS+ 
Sbjct: 397 VCDSLTVPGGVAAVKGAVGIIMQDDSSPDDTNSFPIPASHLGPEAGALILSYINSTNSIP 456

Query: 421 TATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAKD 480
           TATI KSTERKRKRAP                   PDLSGPGVEILAAW     PSGA++
Sbjct: 457 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPISPPSGAEE 516

Query: 481 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRNPAGEF 540
           D+KRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSP+ALKSAL+TTAF MS K N   EF
Sbjct: 517 DSKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEF 576

Query: 541 AYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVFD 600
            YGAGHINPLGAVHPGLIY+ASEIDY+ FLCGQGYTTELLQ+VSEDN TCS  NSDTVFD
Sbjct: 577 GYGAGHINPLGAVHPGLIYDASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFD 636

Query: 601 LNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVNPSKNLEITVKPSVLSFKNLG 660
           LNYPSFALST  SKPINQ+YRR+VTNVGSK ATYKATI+NP KNLEI V PSVLSFKNLG
Sbjct: 637 LNYPSFALSTNISKPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLG 696

Query: 661 EEQSFELTIKGRIGKNIESASLVWDDGRHKVKSPITVFDANIHST 688
           EEQSFE+TIKG+I KNIESASLVWDDG+HKV+SPITVFDANIHS+
Sbjct: 697 EEQSFEVTIKGKIRKNIESASLVWDDGKHKVRSPITVFDANIHSS 741

BLAST of Lag0039317 vs. NCBI nr
Match: KAE8648818.1 (hypothetical protein Csa_008881 [Cucumis sativus])

HSP 1 Score: 1151.3 bits (2977), Expect = 0.0e+00
Identity = 575/706 (81.44%), Postives = 622/706 (88.10%), Query Frame = 0

Query: 1   MGSHPKGKVSTPSHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEG 60
           MGSH KGKVST SHHIRLL+ETIGS+F PHSLLHS++RSFNGFV KLTEAE KKVS+MEG
Sbjct: 35  MGSHSKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEVKKVSEMEG 94

Query: 61  VVSVFPNGIKQLHTTRSWDFMGFSEQVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGP 120
           V+SVFPNG KQLHTTRSWDFMGFSEQV RVP+VESN+IVGV DSGIWPESPSFD  GYG 
Sbjct: 95  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAGYGS 154

Query: 121 PPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLV 180
           PP  WKGSCEVSANFSCNNKIIGARSYRS G++PE DI+GP DS+GHGTHTAS VAGGLV
Sbjct: 155 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 214

Query: 181 RQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGAS 240
           R+ SM+GLG GTARGGVPSARIA YKVCWSDGC DADILAAFDDAIADGV+IISGSLG S
Sbjct: 215 RRASMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 274

Query: 241 RVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTK 300
              DYFNDSIAIGSFHAMKKGILTSLAVGNNGPD TTIVNF+PWSLSVAASTTDRKF TK
Sbjct: 275 GARDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 334

Query: 301 VELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIV 360
           VELGDGR F GVS+NTFD+ G QIPLV+AGDIPKAPFD SVSR+CFE TV+ +LVKGKIV
Sbjct: 335 VELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIV 394

Query: 361 VCDSLAVPEGVVAVEGAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINSTNSVA 420
           VCDSL VP GVVAV+GA GIIMQDDS  DDTNS+P+PASHL PK GAL+LSYINSTNS+ 
Sbjct: 395 VCDSLTVPGGVVAVKGAVGIIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIP 454

Query: 421 TATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAKD 480
           TATI KSTERKRKRAP                   PDLSGPGVEILAAW     PSGA++
Sbjct: 455 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEE 514

Query: 481 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRNPAGEF 540
           DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSP+ALKSAL+TTAFPMS K NP  EF
Sbjct: 515 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEF 574

Query: 541 AYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVFD 600
            YGAGHINPLGAVHPGLIY+ASEIDY++FLCGQGYTTELLQ VSEDN TCS  NSDTVFD
Sbjct: 575 GYGAGHINPLGAVHPGLIYDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFD 634

Query: 601 LNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVNPSKNLEITVKPSVLSFKNLG 660
           LNYPSFALST  SKPINQ+Y+R+VTNVGSKYATYKAT++NP KNLEI V PSVLSFKNLG
Sbjct: 635 LNYPSFALSTNISKPINQVYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLG 694

Query: 661 EEQSFELTIKGRIGKNIESASLVWDDGRHKVKSPITVFDAN-IHST 688
           E+QSFE+TI+G+I K+IESASLVWDDG+HKV+SPITVF AN IHS+
Sbjct: 695 EKQSFEVTIRGKIRKDIESASLVWDDGKHKVRSPITVFIANIIHSS 740

BLAST of Lag0039317 vs. NCBI nr
Match: XP_031741129.1 (cucumisin-like [Cucumis sativus])

HSP 1 Score: 1151.3 bits (2977), Expect = 0.0e+00
Identity = 575/706 (81.44%), Postives = 622/706 (88.10%), Query Frame = 0

Query: 1   MGSHPKGKVSTPSHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEG 60
           MGSH KGKVST SHHIRLL+ETIGS+F PHSLLHS++RSFNGFV KLTEAE KKVS+MEG
Sbjct: 38  MGSHSKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEVKKVSEMEG 97

Query: 61  VVSVFPNGIKQLHTTRSWDFMGFSEQVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGP 120
           V+SVFPNG KQLHTTRSWDFMGFSEQV RVP+VESN+IVGV DSGIWPESPSFD  GYG 
Sbjct: 98  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAGYGS 157

Query: 121 PPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLV 180
           PP  WKGSCEVSANFSCNNKIIGARSYRS G++PE DI+GP DS+GHGTHTAS VAGGLV
Sbjct: 158 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 217

Query: 181 RQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGAS 240
           R+ SM+GLG GTARGGVPSARIA YKVCWSDGC DADILAAFDDAIADGV+IISGSLG S
Sbjct: 218 RRASMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 277

Query: 241 RVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTK 300
              DYFNDSIAIGSFHAMKKGILTSLAVGNNGPD TTIVNF+PWSLSVAASTTDRKF TK
Sbjct: 278 GARDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 337

Query: 301 VELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIV 360
           VELGDGR F GVS+NTFD+ G QIPLV+AGDIPKAPFD SVSR+CFE TV+ +LVKGKIV
Sbjct: 338 VELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIV 397

Query: 361 VCDSLAVPEGVVAVEGAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINSTNSVA 420
           VCDSL VP GVVAV+GA GIIMQDDS  DDTNS+P+PASHL PK GAL+LSYINSTNS+ 
Sbjct: 398 VCDSLTVPGGVVAVKGAVGIIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIP 457

Query: 421 TATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAKD 480
           TATI KSTERKRKRAP                   PDLSGPGVEILAAW     PSGA++
Sbjct: 458 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEE 517

Query: 481 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRNPAGEF 540
           DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSP+ALKSAL+TTAFPMS K NP  EF
Sbjct: 518 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEF 577

Query: 541 AYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVFD 600
            YGAGHINPLGAVHPGLIY+ASEIDY++FLCGQGYTTELLQ VSEDN TCS  NSDTVFD
Sbjct: 578 GYGAGHINPLGAVHPGLIYDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFD 637

Query: 601 LNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVNPSKNLEITVKPSVLSFKNLG 660
           LNYPSFALST  SKPINQ+Y+R+VTNVGSKYATYKAT++NP KNLEI V PSVLSFKNLG
Sbjct: 638 LNYPSFALSTNISKPINQVYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLG 697

Query: 661 EEQSFELTIKGRIGKNIESASLVWDDGRHKVKSPITVFDAN-IHST 688
           E+QSFE+TI+G+I K+IESASLVWDDG+HKV+SPITVF AN IHS+
Sbjct: 698 EKQSFEVTIRGKIRKDIESASLVWDDGKHKVRSPITVFIANIIHSS 743

BLAST of Lag0039317 vs. NCBI nr
Match: XP_022957157.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 573/705 (81.28%), Postives = 625/705 (88.65%), Query Frame = 0

Query: 1   MGSHPKGKVSTPSHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEG 60
           MGSHPKGKVST SHHIR L ETIGS F P SL+HSY+RSFNGFV KLTEAEAKKVS+MEG
Sbjct: 36  MGSHPKGKVSTSSHHIRFLTETIGSNFAPRSLIHSYKRSFNGFVAKLTEAEAKKVSEMEG 95

Query: 61  VVSVFPNGIKQLHTTRSWDFMGFSEQVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGP 120
           V+SVFPNG KQLHTTRSWDFMGFSEQV RVP+VESNIIVGVFDSGIWPESPSFDD GYGP
Sbjct: 96  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNIIVGVFDSGIWPESPSFDDAGYGP 155

Query: 121 PPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLV 180
           PP  W+G CEVSANFSCN+KIIGARSYRS G+H E+DI+GP DS+GHGTHTASTVAGGLV
Sbjct: 156 PPAKWRGRCEVSANFSCNDKIIGARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLV 215

Query: 181 RQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGAS 240
           RQ SM+GLG GTARGGVPSARIA YKVCWSDGC DADILAAFDDAIADGV+IISGSLG S
Sbjct: 216 RQASMLGLGLGTARGGVPSARIAAYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGS 275

Query: 241 RVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTK 300
            V DYFNDSIAIGSFHAM+KGILT+LAVGNNGP+ TTIVNF+PWSLSVAASTTDRKF TK
Sbjct: 276 SVKDYFNDSIAIGSFHAMQKGILTTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETK 335

Query: 301 VELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIV 360
           V+LGDGR F+GVS+NTFDLNGTQIPLV+AGDIPKAPFDRSVSRMCFE TV+KE VKGKIV
Sbjct: 336 VKLGDGREFNGVSVNTFDLNGTQIPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIV 395

Query: 361 VCDSLAVPEGVVAVEGAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINSTNSVA 420
           VCDSLAVP GVVAVEGAAGIIMQD+SPQDDTNSYPLPASH+ PK GALILSYINST  V 
Sbjct: 396 VCDSLAVPGGVVAVEGAAGIIMQDESPQDDTNSYPLPASHVGPKPGALILSYINSTR-VP 455

Query: 421 TATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAKD 480
           TA+I+KS  RKR RAP                   PD+SGPGVEILAAWPSTVSPSGA++
Sbjct: 456 TASIMKSRGRKRNRAPFVASFSSRGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEE 515

Query: 481 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRNPAGEF 540
           DNKRVLYN+ISGTSMACPHVTAAAAYVKSFHPTWSPAALKSAL+TTAFPMS KRNP GE 
Sbjct: 516 DNKRVLYNVISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALITTAFPMSHKRNPDGEL 575

Query: 541 AYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVFD 600
           AYGAGHINPLGAVHPGLIYNASE DY+KFLCGQGY+TE L+R+S+DN +CS  NS TVF+
Sbjct: 576 AYGAGHINPLGAVHPGLIYNASETDYVKFLCGQGYSTESLRRLSDDNASCSANNSGTVFE 635

Query: 601 LNYPSFALSTPTSKPINQIYRRSVTNVGSKYAT-YKATIVNPSKNLEITVKPSVLSFKNL 660
           LNYPSFALST  S PI+Q++RR VTNVGS YAT YKA IVNP ++L+ITVKPSVL FK L
Sbjct: 636 LNYPSFALSTNASNPISQVFRRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRL 695

Query: 661 GEEQSFELTIKGRIGKNIESASLVWDDGRHKVKSPITVFDANIHS 687
           GEE SFE+ I G+I K +ESA+LVWDDG+HKV+SPITVF+AN+HS
Sbjct: 696 GEELSFEVRIGGQIRKGVESAALVWDDGKHKVRSPITVFNANLHS 739

BLAST of Lag0039317 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 2.0e-217
Identity = 389/698 (55.73%), Postives = 492/698 (70.49%), Query Frame = 0

Query: 1   MGSHPKGKVSTPSHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEG 60
           MG   +   S   HH  +L + +GSTF P S+LH+Y+RSFNGF VKLTE EA+K++ MEG
Sbjct: 38  MGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEG 97

Query: 61  VVSVFPNGIKQLHTTRSWDFMGFSEQVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGP 120
           VVSVF N + +LHTTRSWDF+GF   V R   VESNI+VGV D+GIWPESPSFDD+G+ P
Sbjct: 98  VVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSP 157

Query: 121 PPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLV 180
           PPP WKG+CE S NF CN KIIGARSY         D+ GP D+NGHGTHTAST AGGLV
Sbjct: 158 PPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLV 217

Query: 181 RQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGAS 240
            Q ++ GLG GTARGGVP ARIA YKVCW+DGC D DILAA+DDAIADGV+IIS S+G +
Sbjct: 218 SQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGA 277

Query: 241 RVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTK 300
               YF D+IAIGSFHA+++GILTS + GN GP+  T  + +PW LSVAAST DRKF T+
Sbjct: 278 NPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQ 337

Query: 301 VELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIV 360
           V++G+G++F GVSINTFD      PLV   DIP   FD+S SR C + +V   L+KGKIV
Sbjct: 338 VQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIV 397

Query: 361 VCDSLAVP-EGVVAVEGAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINSTNSV 420
           VC++   P E   +++GAAG++M  ++ +D  +SYPLP+S L P      L YI S  S 
Sbjct: 398 VCEASFGPHEFFKSLDGAAGVLMTSNT-RDYADSYPLPSSVLDPNDLLATLRYIYSIRSP 457

Query: 421 ATATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAK 480
             ATI KST      AP+                  PD+SGPGVEILAAWPS     G +
Sbjct: 458 G-ATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIR 517

Query: 481 DDNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRNPAGE 540
              +  L+NIISGTSM+CPH+T  A YVK+++PTWSPAA+KSALMTTA PM+ + NP  E
Sbjct: 518 ---RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAE 577

Query: 541 FAYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVF 600
           FAYG+GH+NPL AV PGL+Y+A+E DY+KFLCGQGY T+ ++R++ D + C+  N+  V+
Sbjct: 578 FAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVW 637

Query: 601 DLNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVNPSKNLEITVKPSVLSFKNL 660
           DLNYPSF LS   S+  NQ + R++T+V  + +TY+A I  P + L I+V P+VLSF  L
Sbjct: 638 DLNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAP-QGLTISVNPNVLSFNGL 697

Query: 661 GEEQSFELTIKGRIGKNIESASLVWDDGRHKVKSPITV 680
           G+ +SF LT++G I   + SASLVW DG H V+SPIT+
Sbjct: 698 GDRKSFTLTVRGSIKGFVVSASLVWSDGVHYVRSPITI 727

BLAST of Lag0039317 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 6.6e-181
Identity = 341/703 (48.51%), Postives = 456/703 (64.86%), Query Frame = 0

Query: 1   MGSHPKGKVSTPSHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEG 60
           MG+ P+ K S PSHH+ +L++ +G+    H L+ SY+RSFNGF   L++AE++K+  M+ 
Sbjct: 37  MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 96

Query: 61  VVSVFPNGIKQLHTTRSWDFMGFSEQVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGP 120
           VVSVFP+   +L TTRSWDF+GF E+  R    ES++IVGV DSGIWPES SFDD+G+GP
Sbjct: 97  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 156

Query: 121 PPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLV 180
           PP  WKGSC+    F+CNNK+IGAR Y        D+        GHGTHTAST AG  V
Sbjct: 157 PPKKWKGSCKGGLKFACNNKLIGARFYNKFADSARDE-------EGHGTHTASTAAGNAV 216

Query: 181 RQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGAS 240
           +  S  GL +GTARGGVPSARIA YKVC+ + C+D DILAAFDDAIADGV++IS S+ A 
Sbjct: 217 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 276

Query: 241 RVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTK 300
            V +  N S+AIGSFHAM +GI+T+ + GNNGPD  ++ N +PW ++VAAS TDR+F  +
Sbjct: 277 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 336

Query: 301 VELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIV 360
           V LG+G+A  G+S+NTF+LNGT+ P+V+  ++ +    ++ +  C  G V+ ELVKGKIV
Sbjct: 337 VVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSR-NCSQAQAGYCSSGCVDSELVKGKIV 396

Query: 361 VCDS-LAVPEGVVAVEGAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINSTNSV 420
           +CD  L   E  +A  GA G+I+Q+    D     P PAS L  +    I SYI S    
Sbjct: 397 LCDDFLGYREAYLA--GAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP- 456

Query: 421 ATATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSG-- 480
             A IL++ E   + AP                   PD+S PG+EILAA+    SPS   
Sbjct: 457 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 516

Query: 481 AKDDNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRNPA 540
             +D + V Y+++SGTSMACPHV   AAYVKSFHP WSP+A+KSA+MTTA PM+LK+NP 
Sbjct: 517 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 576

Query: 541 GEFAYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDT 600
            EFAYG+G INP  A  PGL+Y     DY+K LC +G+ +  L   S  N TCS +    
Sbjct: 577 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTE-- 636

Query: 601 VFDLNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVNPSKNLEITVKPSVLSFK 660
           V DLNYP+      +  P N  ++R+VTNVG   +TYKA++V     L+I+++P +L F 
Sbjct: 637 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 696

Query: 661 NLGEEQSFELTIKGRIGK--NIESASLVWDDGRHKVKSPITVF 681
            L E++SF +TI G+  K  +  S+S+VW DG H V+SPI  +
Sbjct: 697 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 725

BLAST of Lag0039317 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 3.1e-170
Identity = 327/709 (46.12%), Postives = 460/709 (64.88%), Query Frame = 0

Query: 1   MGSHPKGKVSTP-SHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKME 60
           +GS P  +  TP S H+ +L+E  G +   + L+ SY++SFNGF  +LTE+E K+++ ME
Sbjct: 39  LGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGME 98

Query: 61  GVVSVFPNGIKQLHTTRSWDFMGFSE--QVDRVPSVESNIIVGVFDSGIWPESPSFDDKG 120
            VVSVFP+   +L TT SW+FMG  E  +  R  S+ES+ I+GV DSGI+PES SF D+G
Sbjct: 99  RVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQG 158

Query: 121 YGPPPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAG 180
           +GPPP  WKG+C    NF+CNNK+IGAR Y +  +  +       D +GHGTHTAS  AG
Sbjct: 159 FGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKSKANQT----ARDYSGHGTHTASIAAG 218

Query: 181 GLVRQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSL 240
             V   +  GLG GTARGGVP+ARIAVYKVC ++GC    +++AFDDAIADGV++IS S+
Sbjct: 219 NAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISI 278

Query: 241 GASRVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKF 300
               +  +  D IAIG+FHAM  G+LT  A GNNGP ++T+ + APW  SVAAS T+R F
Sbjct: 279 VLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAF 338

Query: 301 TTKVELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKG 360
             KV LGDG+   G S+NT+D+NGT  PLV+      +      +R+C    ++ +LVKG
Sbjct: 339 MAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKG 398

Query: 361 KIVVCDSLAVPEGVVAVE--GAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINS 420
           KIV+CDS    +G++  +  GA G I+++  P D       P S L       ++SY+NS
Sbjct: 399 KIVLCDS---TKGLIEAQKLGAVGSIVKNPEP-DRAFIRSFPVSFLSNDDYKSLVSYMNS 458

Query: 421 TNSVATATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSP 480
           T +   AT+LKS E   +RAP+                  PD++ PGVEILAA+    SP
Sbjct: 459 TKN-PKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSP 518

Query: 481 SGAKDDNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRN 540
           + ++ D +RV Y+++SGTSMACPHV   AAYVK+FHP WSP+ ++SA+MTTA+PM+   +
Sbjct: 519 TESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGS 578

Query: 541 --PAGEFAYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLK 600
              + EFAYG+GH++P+ A++PGL+Y  ++ D+I FLCG  YT++ L+ +S DN+TC+ +
Sbjct: 579 GFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKE 638

Query: 601 NSDTV-FDLNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVN-PSKNLEITVKP 660
            S T+  +LNYP+ +     +KP N  ++R+VTNVG + +TY A +V  P   L I V P
Sbjct: 639 ISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSP 698

Query: 661 SVLSFKNLGEEQSFELTIKG-RIG-KNIESASLVWDDGRHKVKSPITVF 681
            VLS K++ E+QSF +T+    IG K   SA+L+W DG H V+SPI V+
Sbjct: 699 RVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSDGTHNVRSPIIVY 738

BLAST of Lag0039317 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 2.1e-166
Identity = 329/710 (46.34%), Postives = 450/710 (63.38%), Query Frame = 0

Query: 1   MGSHPKGKVSTP-SHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKME 60
           MG+ P      P SHH  +L++  G +     L+ +Y+RSFNGF  +LTE+E + ++ M+
Sbjct: 38  MGALPSRVDYMPMSHHTSILQDVTGESSIQDRLVRNYKRSFNGFAARLTESEREILASMD 97

Query: 61  GVVSVFPNGIKQLHTTRSWDFMGFSE--QVDRVPSVESNIIVGVFDSGIWPESPSFDDKG 120
            VVSVFP+    L TT SW+FMG  E  +  R P +ES+ I+GV DSGI+PES SF  KG
Sbjct: 98  EVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKG 157

Query: 121 YGPPPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAG 180
           +GPPP  WKG C+   NF+CNNK+IGAR Y    +   +  R   D+ GHG+HTAS  AG
Sbjct: 158 FGPPPKKWKGVCKGGTNFTCNNKLIGARYYTPKLEGFPESAR---DNTGHGSHTASIAAG 217

Query: 181 GLVRQVSMMGLGRGTARGGVPSARIAVYKVCWSD--GCHDADILAAFDDAIADGVNIISG 240
             V+ VS  GLG GT RGGVP+ARIAVYKVC      C    ILAAFDDAIAD V+II+ 
Sbjct: 218 NAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITV 277

Query: 241 SLGASRVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDR 300
           SLGA  VG +  D++AIG+FHAM KGILT    GNNGP+  TIV+ APW  +VAAS  +R
Sbjct: 278 SLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNR 337

Query: 301 KFTTKVELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELV 360
            F TKV LG+G+   G S+N+FDLNG + PLV+ G    +  D S +  C  G ++ + V
Sbjct: 338 AFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVY-GKSASSRCDASSAGFCSPGCLDSKRV 397

Query: 361 KGKIVVCDSLAVPEGVVAVEGAAGIIMQDDSPQDDTNS-YPLPASHLPPKQGALILSYIN 420
           KGKIV+CD+   P    A+   A I+    +P +D  S +  P S L      ++LSY+N
Sbjct: 398 KGKIVLCDTQRNPGEAQAMGAVASIVR---NPYEDAASVFSFPVSVLSEDDYNIVLSYVN 457

Query: 421 STNSVATATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVS 480
           ST +   A +LKS     ++AP+                  PD++ PG EILAA+   V 
Sbjct: 458 STKN-PKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVP 517

Query: 481 PSGAKDDNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKR 540
           PS  + D + V Y +ISGTSM+CPHV   AAY+K+FHP WSP+ ++SA+MTTA+PM+   
Sbjct: 518 PS--ESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNAST 577

Query: 541 NPA---GEFAYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCS 600
           +P+    EFAYGAGH++P+ A+HPGL+Y A++ D+I FLCG  YT + L+ +S D+++C+
Sbjct: 578 SPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCT 637

Query: 601 LKNSDTVF-DLNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVNPSKNLEITVK 660
            + + ++  +LNYPS +     +KP    +RR+VTNVG   ATYKA +V     L++ V 
Sbjct: 638 KEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVV--GSKLKVKVV 697

Query: 661 PSVLSFKNLGEEQSFELTIKGRIGK--NIESASLVWDDGRHKVKSPITVF 681
           P+VLS K+L E++SF +T+ G   K  N+ SA L+W DG H V+SPI V+
Sbjct: 698 PAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of Lag0039317 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 2.1e-166
Identity = 318/695 (45.76%), Postives = 433/695 (62.30%), Query Frame = 0

Query: 13  SHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEGVVSVFPNGIKQL 72
           S H+ +L++  G +     L+ SY+RSFNGF  +LTE+E   ++++EGVVSVFPN I QL
Sbjct: 50  SDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQL 109

Query: 73  HTTRSWDFMGFSE--QVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGPPPPNWKGSCE 132
           HTT SWDFMG  E     R  ++ES+ I+GV D+GIWPES SF DKG+GPPP  WKG C 
Sbjct: 110 HTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCS 169

Query: 133 VSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLVRQVSMMGLGR 192
              NF+CNNK+IGAR Y S G           D++GHGTHTAST AG  V+  S  G+G 
Sbjct: 170 GGKNFTCNNKLIGARDYTSEGTR---------DTSGHGTHTASTAAGNAVKDTSFFGIGN 229

Query: 193 GTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGASRVGDYFNDSI 252
           GT RGGVP++RIA YKVC   GC    +L++FDDAIADGV++I+ S+G      + +D I
Sbjct: 230 GTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPI 289

Query: 253 AIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTKVELGDGRAFD 312
           AIG+FHAM KGILT  + GN+GP  TT+ + APW  +VAASTT+R F TKV LG+G+   
Sbjct: 290 AIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLA 349

Query: 313 GVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIVVCDSLAVPEG 372
           G S+N FD+ G + PLV+      +  D   + +C    + K  VKGKI+VC     P G
Sbjct: 350 GRSVNAFDMKGKKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGG---PSG 409

Query: 373 VVAVEGAAGIIMQDDSPQDDTN-SYPLPASHLPPKQGALILSYINSTNSVATATILKSTE 432
               +    I + D SP+ D   ++ LPAS L  K    ++SYI S +S   A +LK+  
Sbjct: 410 YKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDS-PQAAVLKTET 469

Query: 433 RKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAKDDNKRVLYNI 492
              + +P+                  PD++ PGVEILAA+     PS  +DD +RV Y++
Sbjct: 470 IFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSV 529

Query: 493 ISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRN--PAGEFAYGAGHI 552
            SGTSMACPHV   AAYVK+F+P WSP+ ++SA+MTTA+P+  K     + EFAYGAGH+
Sbjct: 530 FSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHV 589

Query: 553 NPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVFDLNYPSF- 612
           +P+ A++PGL+Y   + D+I FLCG  YT++ L+ +S D   CS KN     +LNYPS  
Sbjct: 590 DPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMS 649

Query: 613 ALSTPTSKPINQIYRRSVTNVGSKYATYKATIV-NPSKNLEITVKPSVLSFKNLGEEQSF 672
           A  + T    +  + R++TNVG+  +TYK+ +V      L I V PSVL FK + E+QSF
Sbjct: 650 AKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSF 709

Query: 673 ELTIKGR-IGKNI-ESASLVWDDGRHKVKSPITVF 681
            +T+ G  +   +  SA+L+W DG H V+SPI V+
Sbjct: 710 SVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 729

BLAST of Lag0039317 vs. ExPASy TrEMBL
Match: A0A5A7SZX9 (Cucumisin-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G006680 PE=3 SV=1)

HSP 1 Score: 1158.3 bits (2995), Expect = 0.0e+00
Identity = 578/705 (81.99%), Postives = 623/705 (88.37%), Query Frame = 0

Query: 1   MGSHPKGKVSTPSHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEG 60
           MGSHPKGKVST SHHIRLL+ETIGS+F PHSLLHS++RSFNGFV KLTEAEAKKVS+MEG
Sbjct: 37  MGSHPKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEAKKVSEMEG 96

Query: 61  VVSVFPNGIKQLHTTRSWDFMGFSEQVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGP 120
           V+SVFPNG KQLHTTRSWDFMGFSEQV RVP+VESN+IVGV DSGIWPESPSFD  GYGP
Sbjct: 97  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTGYGP 156

Query: 121 PPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLV 180
           PP  WKGSCEVSANFSCNNKIIGARSYRS G++PE DI+GP DS+GHGTHTAS VAGGLV
Sbjct: 157 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 216

Query: 181 RQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGAS 240
           RQ +M+GLG GTARGGVPSARIA YKVCWSDGC DADILAAFDDAIADGV+IISGSLG S
Sbjct: 217 RQANMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 276

Query: 241 RVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTK 300
              DYFNDSIAIGSFHAMKKGILTSLAVGNNGPD TTIVNF+PWSLSVAASTTDRKF TK
Sbjct: 277 GFRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 336

Query: 301 VELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIV 360
           VELGDGR F+GVS+NTFD+ G QIPLV+AGDIPKAPFD SVSR+CFE T++ +LVKGKIV
Sbjct: 337 VELGDGREFNGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTIDLKLVKGKIV 396

Query: 361 VCDSLAVPEGVVAVEGAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINSTNSVA 420
           VCDSL VP GV AV+GA GIIMQDDS  DDTNS+P+PASHL P+ GALILSYINSTNS+ 
Sbjct: 397 VCDSLTVPGGVAAVKGAVGIIMQDDSSPDDTNSFPIPASHLGPEAGALILSYINSTNSIP 456

Query: 421 TATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAKD 480
           TATI KSTERKRKRAP                   PDLSGPGVEILAAW     PSGA++
Sbjct: 457 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPISPPSGAEE 516

Query: 481 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRNPAGEF 540
           D+KRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSP+ALKSAL+TTAF MS K N   EF
Sbjct: 517 DSKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEF 576

Query: 541 AYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVFD 600
            YGAGHINPLGAVHPGLIY+ASEIDY+ FLCGQGYTTELLQ+VSEDN TCS  NSDTVFD
Sbjct: 577 GYGAGHINPLGAVHPGLIYDASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFD 636

Query: 601 LNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVNPSKNLEITVKPSVLSFKNLG 660
           LNYPSFALST  SKPINQ+YRR+VTNVGSK ATYKATI+NP KNLEI V PSVLSFKNLG
Sbjct: 637 LNYPSFALSTNISKPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLG 696

Query: 661 EEQSFELTIKGRIGKNIESASLVWDDGRHKVKSPITVFDANIHST 688
           EEQSFE+TIKG+I KNIESASLVWDDG+HKV+SPITVFDANIHS+
Sbjct: 697 EEQSFEVTIKGKIRKNIESASLVWDDGKHKVRSPITVFDANIHSS 741

BLAST of Lag0039317 vs. ExPASy TrEMBL
Match: A0A1S4DW16 (cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489381 PE=3 SV=1)

HSP 1 Score: 1158.3 bits (2995), Expect = 0.0e+00
Identity = 578/705 (81.99%), Postives = 623/705 (88.37%), Query Frame = 0

Query: 1   MGSHPKGKVSTPSHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEG 60
           MGSHPKGKVST SHHIRLL+ETIGS+F PHSLLHS++RSFNGFV KLTEAEAKKVS+MEG
Sbjct: 37  MGSHPKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEAKKVSEMEG 96

Query: 61  VVSVFPNGIKQLHTTRSWDFMGFSEQVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGP 120
           V+SVFPNG KQLHTTRSWDFMGFSEQV RVP+VESN+IVGV DSGIWPESPSFD  GYGP
Sbjct: 97  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTGYGP 156

Query: 121 PPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLV 180
           PP  WKGSCEVSANFSCNNKIIGARSYRS G++PE DI+GP DS+GHGTHTAS VAGGLV
Sbjct: 157 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 216

Query: 181 RQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGAS 240
           RQ +M+GLG GTARGGVPSARIA YKVCWSDGC DADILAAFDDAIADGV+IISGSLG S
Sbjct: 217 RQANMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 276

Query: 241 RVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTK 300
              DYFNDSIAIGSFHAMKKGILTSLAVGNNGPD TTIVNF+PWSLSVAASTTDRKF TK
Sbjct: 277 GFRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 336

Query: 301 VELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIV 360
           VELGDGR F+GVS+NTFD+ G QIPLV+AGDIPKAPFD SVSR+CFE T++ +LVKGKIV
Sbjct: 337 VELGDGREFNGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTIDLKLVKGKIV 396

Query: 361 VCDSLAVPEGVVAVEGAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINSTNSVA 420
           VCDSL VP GV AV+GA GIIMQDDS  DDTNS+P+PASHL P+ GALILSYINSTNS+ 
Sbjct: 397 VCDSLTVPGGVAAVKGAVGIIMQDDSSPDDTNSFPIPASHLGPEAGALILSYINSTNSIP 456

Query: 421 TATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAKD 480
           TATI KSTERKRKRAP                   PDLSGPGVEILAAW     PSGA++
Sbjct: 457 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPISPPSGAEE 516

Query: 481 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRNPAGEF 540
           D+KRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSP+ALKSAL+TTAF MS K N   EF
Sbjct: 517 DSKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEF 576

Query: 541 AYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVFD 600
            YGAGHINPLGAVHPGLIY+ASEIDY+ FLCGQGYTTELLQ+VSEDN TCS  NSDTVFD
Sbjct: 577 GYGAGHINPLGAVHPGLIYDASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFD 636

Query: 601 LNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVNPSKNLEITVKPSVLSFKNLG 660
           LNYPSFALST  SKPINQ+YRR+VTNVGSK ATYKATI+NP KNLEI V PSVLSFKNLG
Sbjct: 637 LNYPSFALSTNISKPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLG 696

Query: 661 EEQSFELTIKGRIGKNIESASLVWDDGRHKVKSPITVFDANIHST 688
           EEQSFE+TIKG+I KNIESASLVWDDG+HKV+SPITVFDANIHS+
Sbjct: 697 EEQSFEVTIKGKIRKNIESASLVWDDGKHKVRSPITVFDANIHSS 741

BLAST of Lag0039317 vs. ExPASy TrEMBL
Match: A0A0A0KWL9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G613590 PE=3 SV=1)

HSP 1 Score: 1151.3 bits (2977), Expect = 0.0e+00
Identity = 575/706 (81.44%), Postives = 622/706 (88.10%), Query Frame = 0

Query: 1   MGSHPKGKVSTPSHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEG 60
           MGSH KGKVST SHHIRLL+ETIGS+F PHSLLHS++RSFNGFV KLTEAE KKVS+MEG
Sbjct: 38  MGSHSKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEVKKVSEMEG 97

Query: 61  VVSVFPNGIKQLHTTRSWDFMGFSEQVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGP 120
           V+SVFPNG KQLHTTRSWDFMGFSEQV RVP+VESN+IVGV DSGIWPESPSFD  GYG 
Sbjct: 98  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAGYGS 157

Query: 121 PPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLV 180
           PP  WKGSCEVSANFSCNNKIIGARSYRS G++PE DI+GP DS+GHGTHTAS VAGGLV
Sbjct: 158 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 217

Query: 181 RQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGAS 240
           R+ SM+GLG GTARGGVPSARIA YKVCWSDGC DADILAAFDDAIADGV+IISGSLG S
Sbjct: 218 RRASMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 277

Query: 241 RVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTK 300
              DYFNDSIAIGSFHAMKKGILTSLAVGNNGPD TTIVNF+PWSLSVAASTTDRKF TK
Sbjct: 278 GARDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 337

Query: 301 VELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIV 360
           VELGDGR F GVS+NTFD+ G QIPLV+AGDIPKAPFD SVSR+CFE TV+ +LVKGKIV
Sbjct: 338 VELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIV 397

Query: 361 VCDSLAVPEGVVAVEGAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINSTNSVA 420
           VCDSL VP GVVAV+GA GIIMQDDS  DDTNS+P+PASHL PK GAL+LSYINSTNS+ 
Sbjct: 398 VCDSLTVPGGVVAVKGAVGIIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIP 457

Query: 421 TATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAKD 480
           TATI KSTERKRKRAP                   PDLSGPGVEILAAW     PSGA++
Sbjct: 458 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEE 517

Query: 481 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRNPAGEF 540
           DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSP+ALKSAL+TTAFPMS K NP  EF
Sbjct: 518 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEF 577

Query: 541 AYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVFD 600
            YGAGHINPLGAVHPGLIY+ASEIDY++FLCGQGYTTELLQ VSEDN TCS  NSDTVFD
Sbjct: 578 GYGAGHINPLGAVHPGLIYDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFD 637

Query: 601 LNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVNPSKNLEITVKPSVLSFKNLG 660
           LNYPSFALST  SKPINQ+Y+R+VTNVGSKYATYKAT++NP KNLEI V PSVLSFKNLG
Sbjct: 638 LNYPSFALSTNISKPINQVYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLG 697

Query: 661 EEQSFELTIKGRIGKNIESASLVWDDGRHKVKSPITVFDAN-IHST 688
           E+QSFE+TI+G+I K+IESASLVWDDG+HKV+SPITVF AN IHS+
Sbjct: 698 EKQSFEVTIRGKIRKDIESASLVWDDGKHKVRSPITVFIANIIHSS 743

BLAST of Lag0039317 vs. ExPASy TrEMBL
Match: A0A6J1GYD4 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458626 PE=3 SV=1)

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 573/705 (81.28%), Postives = 625/705 (88.65%), Query Frame = 0

Query: 1   MGSHPKGKVSTPSHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEG 60
           MGSHPKGKVST SHHIR L ETIGS F P SL+HSY+RSFNGFV KLTEAEAKKVS+MEG
Sbjct: 36  MGSHPKGKVSTSSHHIRFLTETIGSNFAPRSLIHSYKRSFNGFVAKLTEAEAKKVSEMEG 95

Query: 61  VVSVFPNGIKQLHTTRSWDFMGFSEQVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGP 120
           V+SVFPNG KQLHTTRSWDFMGFSEQV RVP+VESNIIVGVFDSGIWPESPSFDD GYGP
Sbjct: 96  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNIIVGVFDSGIWPESPSFDDAGYGP 155

Query: 121 PPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLV 180
           PP  W+G CEVSANFSCN+KIIGARSYRS G+H E+DI+GP DS+GHGTHTASTVAGGLV
Sbjct: 156 PPAKWRGRCEVSANFSCNDKIIGARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLV 215

Query: 181 RQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGAS 240
           RQ SM+GLG GTARGGVPSARIA YKVCWSDGC DADILAAFDDAIADGV+IISGSLG S
Sbjct: 216 RQASMLGLGLGTARGGVPSARIAAYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGS 275

Query: 241 RVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTK 300
            V DYFNDSIAIGSFHAM+KGILT+LAVGNNGP+ TTIVNF+PWSLSVAASTTDRKF TK
Sbjct: 276 SVKDYFNDSIAIGSFHAMQKGILTTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETK 335

Query: 301 VELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIV 360
           V+LGDGR F+GVS+NTFDLNGTQIPLV+AGDIPKAPFDRSVSRMCFE TV+KE VKGKIV
Sbjct: 336 VKLGDGREFNGVSVNTFDLNGTQIPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIV 395

Query: 361 VCDSLAVPEGVVAVEGAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINSTNSVA 420
           VCDSLAVP GVVAVEGAAGIIMQD+SPQDDTNSYPLPASH+ PK GALILSYINST  V 
Sbjct: 396 VCDSLAVPGGVVAVEGAAGIIMQDESPQDDTNSYPLPASHVGPKPGALILSYINSTR-VP 455

Query: 421 TATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAKD 480
           TA+I+KS  RKR RAP                   PD+SGPGVEILAAWPSTVSPSGA++
Sbjct: 456 TASIMKSRGRKRNRAPFVASFSSRGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEE 515

Query: 481 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRNPAGEF 540
           DNKRVLYN+ISGTSMACPHVTAAAAYVKSFHPTWSPAALKSAL+TTAFPMS KRNP GE 
Sbjct: 516 DNKRVLYNVISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALITTAFPMSHKRNPDGEL 575

Query: 541 AYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVFD 600
           AYGAGHINPLGAVHPGLIYNASE DY+KFLCGQGY+TE L+R+S+DN +CS  NS TVF+
Sbjct: 576 AYGAGHINPLGAVHPGLIYNASETDYVKFLCGQGYSTESLRRLSDDNASCSANNSGTVFE 635

Query: 601 LNYPSFALSTPTSKPINQIYRRSVTNVGSKYAT-YKATIVNPSKNLEITVKPSVLSFKNL 660
           LNYPSFALST  S PI+Q++RR VTNVGS YAT YKA IVNP ++L+ITVKPSVL FK L
Sbjct: 636 LNYPSFALSTNASNPISQVFRRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRL 695

Query: 661 GEEQSFELTIKGRIGKNIESASLVWDDGRHKVKSPITVFDANIHS 687
           GEE SFE+ I G+I K +ESA+LVWDDG+HKV+SPITVF+AN+HS
Sbjct: 696 GEELSFEVRIGGQIRKGVESAALVWDDGKHKVRSPITVFNANLHS 739

BLAST of Lag0039317 vs. ExPASy TrEMBL
Match: A0A5D3CCZ5 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001930 PE=3 SV=1)

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 570/698 (81.66%), Postives = 615/698 (88.11%), Query Frame = 0

Query: 1   MGSHPKGKVSTPSHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEG 60
           MGSHPKGKVST SHHIRLL+ETIGS+F PHSLLHS++RSFNGFV KLTEAEAKKVS+MEG
Sbjct: 37  MGSHPKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEAKKVSEMEG 96

Query: 61  VVSVFPNGIKQLHTTRSWDFMGFSEQVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGP 120
           V+SVFPNG KQLHTTRSWDFMGFSEQV RVP+VESN+IVGV DSGIWPESPSFD  GYGP
Sbjct: 97  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTGYGP 156

Query: 121 PPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLV 180
           PP  WKGSCEVSANFSCNNKIIGARSYRS G++PE DI+GP DS+GHGTHTAS VAGGLV
Sbjct: 157 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 216

Query: 181 RQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGAS 240
           RQ +M+GLG GTARGGVPSARIA YKVCWSDGC DADILAAFDDAIADGV+IISGSLG S
Sbjct: 217 RQANMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 276

Query: 241 RVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTK 300
              DYFNDSIAIGSFHAMKKGILTSLAVGNNGPD TTIVNF+PWSLSVAASTTDRKF TK
Sbjct: 277 GFRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 336

Query: 301 VELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIV 360
           VELGDGR F+GVS+NTFD+ G QIPLV+AGDIPKAPFD SVSR+CFE T++ +LVKGKIV
Sbjct: 337 VELGDGREFNGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTIDLKLVKGKIV 396

Query: 361 VCDSLAVPEGVVAVEGAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINSTNSVA 420
           VCDSL VP GV AV+GA GIIMQDDS  DDTNS+P+PASHL P+ GALILSYINSTNS+ 
Sbjct: 397 VCDSLTVPGGVAAVKGAVGIIMQDDSSPDDTNSFPIPASHLGPEAGALILSYINSTNSIP 456

Query: 421 TATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAKD 480
           TATI KSTERKRKRAP                   PDLSGPGVEILAAW     PSGA++
Sbjct: 457 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPISPPSGAEE 516

Query: 481 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRNPAGEF 540
           D+KRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSP+ALKSAL+TTAF MS K N   EF
Sbjct: 517 DSKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEF 576

Query: 541 AYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVFD 600
            YGAGHINPLGAVHPGLIY+ASEIDY+ FLCGQGYTTELLQ+VSEDN TCS  NSDTVFD
Sbjct: 577 GYGAGHINPLGAVHPGLIYDASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFD 636

Query: 601 LNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVNPSKNLEITVKPSVLSFKNLG 660
           LNYPSFALST  SKPINQ+YRR+VTNVGSK ATYKATI+NP KNLEI V PSVLSFKNLG
Sbjct: 637 LNYPSFALSTNISKPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLG 696

Query: 661 EEQSFELTIKGRIGKNIESASLVWDDGRHKVKSPITVF 681
           EEQSFE+TIKG+I KNIESASLVWDDG+HKV+SPIT +
Sbjct: 697 EEQSFEVTIKGKIRKNIESASLVWDDGKHKVRSPITTY 734

BLAST of Lag0039317 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 635.6 bits (1638), Expect = 4.7e-182
Identity = 341/703 (48.51%), Postives = 456/703 (64.86%), Query Frame = 0

Query: 1   MGSHPKGKVSTPSHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEG 60
           MG+ P+ K S PSHH+ +L++ +G+    H L+ SY+RSFNGF   L++AE++K+  M+ 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 61  VVSVFPNGIKQLHTTRSWDFMGFSEQVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGP 120
           VVSVFP+   +L TTRSWDF+GF E+  R    ES++IVGV DSGIWPES SFDD+G+GP
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 121 PPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLV 180
           PP  WKGSC+    F+CNNK+IGAR Y        D+        GHGTHTAST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYNKFADSARDE-------EGHGTHTASTAAGNAV 180

Query: 181 RQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGAS 240
           +  S  GL +GTARGGVPSARIA YKVC+ + C+D DILAAFDDAIADGV++IS S+ A 
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 241 RVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTK 300
            V +  N S+AIGSFHAM +GI+T+ + GNNGPD  ++ N +PW ++VAAS TDR+F  +
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 301 VELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIV 360
           V LG+G+A  G+S+NTF+LNGT+ P+V+  ++ +    ++ +  C  G V+ ELVKGKIV
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSR-NCSQAQAGYCSSGCVDSELVKGKIV 360

Query: 361 VCDS-LAVPEGVVAVEGAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINSTNSV 420
           +CD  L   E  +A  GA G+I+Q+    D     P PAS L  +    I SYI S    
Sbjct: 361 LCDDFLGYREAYLA--GAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP- 420

Query: 421 ATATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSG-- 480
             A IL++ E   + AP                   PD+S PG+EILAA+    SPS   
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480

Query: 481 AKDDNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRNPA 540
             +D + V Y+++SGTSMACPHV   AAYVKSFHP WSP+A+KSA+MTTA PM+LK+NP 
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540

Query: 541 GEFAYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDT 600
            EFAYG+G INP  A  PGL+Y     DY+K LC +G+ +  L   S  N TCS +    
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTE-- 600

Query: 601 VFDLNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVNPSKNLEITVKPSVLSFK 660
           V DLNYP+      +  P N  ++R+VTNVG   +TYKA++V     L+I+++P +L F 
Sbjct: 601 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 660

Query: 661 NLGEEQSFELTIKGRIGK--NIESASLVWDDGRHKVKSPITVF 681
            L E++SF +TI G+  K  +  S+S+VW DG H V+SPI  +
Sbjct: 661 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 689

BLAST of Lag0039317 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 600.1 bits (1546), Expect = 2.2e-171
Identity = 327/709 (46.12%), Postives = 460/709 (64.88%), Query Frame = 0

Query: 1   MGSHPKGKVSTP-SHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKME 60
           +GS P  +  TP S H+ +L+E  G +   + L+ SY++SFNGF  +LTE+E K+++ ME
Sbjct: 39  LGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGME 98

Query: 61  GVVSVFPNGIKQLHTTRSWDFMGFSE--QVDRVPSVESNIIVGVFDSGIWPESPSFDDKG 120
            VVSVFP+   +L TT SW+FMG  E  +  R  S+ES+ I+GV DSGI+PES SF D+G
Sbjct: 99  RVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQG 158

Query: 121 YGPPPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAG 180
           +GPPP  WKG+C    NF+CNNK+IGAR Y +  +  +       D +GHGTHTAS  AG
Sbjct: 159 FGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKSKANQT----ARDYSGHGTHTASIAAG 218

Query: 181 GLVRQVSMMGLGRGTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSL 240
             V   +  GLG GTARGGVP+ARIAVYKVC ++GC    +++AFDDAIADGV++IS S+
Sbjct: 219 NAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISI 278

Query: 241 GASRVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKF 300
               +  +  D IAIG+FHAM  G+LT  A GNNGP ++T+ + APW  SVAAS T+R F
Sbjct: 279 VLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAF 338

Query: 301 TTKVELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKG 360
             KV LGDG+   G S+NT+D+NGT  PLV+      +      +R+C    ++ +LVKG
Sbjct: 339 MAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKG 398

Query: 361 KIVVCDSLAVPEGVVAVE--GAAGIIMQDDSPQDDTNSYPLPASHLPPKQGALILSYINS 420
           KIV+CDS    +G++  +  GA G I+++  P D       P S L       ++SY+NS
Sbjct: 399 KIVLCDS---TKGLIEAQKLGAVGSIVKNPEP-DRAFIRSFPVSFLSNDDYKSLVSYMNS 458

Query: 421 TNSVATATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVSP 480
           T +   AT+LKS E   +RAP+                  PD++ PGVEILAA+    SP
Sbjct: 459 TKN-PKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSP 518

Query: 481 SGAKDDNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRN 540
           + ++ D +RV Y+++SGTSMACPHV   AAYVK+FHP WSP+ ++SA+MTTA+PM+   +
Sbjct: 519 TESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGS 578

Query: 541 --PAGEFAYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLK 600
              + EFAYG+GH++P+ A++PGL+Y  ++ D+I FLCG  YT++ L+ +S DN+TC+ +
Sbjct: 579 GFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKE 638

Query: 601 NSDTV-FDLNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVN-PSKNLEITVKP 660
            S T+  +LNYP+ +     +KP N  ++R+VTNVG + +TY A +V  P   L I V P
Sbjct: 639 ISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSP 698

Query: 661 SVLSFKNLGEEQSFELTIKG-RIG-KNIESASLVWDDGRHKVKSPITVF 681
            VLS K++ E+QSF +T+    IG K   SA+L+W DG H V+SPI V+
Sbjct: 699 RVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSDGTHNVRSPIIVY 738

BLAST of Lag0039317 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 587.4 bits (1513), Expect = 1.5e-167
Identity = 329/710 (46.34%), Postives = 450/710 (63.38%), Query Frame = 0

Query: 1   MGSHPKGKVSTP-SHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKME 60
           MG+ P      P SHH  +L++  G +     L+ +Y+RSFNGF  +LTE+E + ++ M+
Sbjct: 38  MGALPSRVDYMPMSHHTSILQDVTGESSIQDRLVRNYKRSFNGFAARLTESEREILASMD 97

Query: 61  GVVSVFPNGIKQLHTTRSWDFMGFSE--QVDRVPSVESNIIVGVFDSGIWPESPSFDDKG 120
            VVSVFP+    L TT SW+FMG  E  +  R P +ES+ I+GV DSGI+PES SF  KG
Sbjct: 98  EVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKG 157

Query: 121 YGPPPPNWKGSCEVSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAG 180
           +GPPP  WKG C+   NF+CNNK+IGAR Y    +   +  R   D+ GHG+HTAS  AG
Sbjct: 158 FGPPPKKWKGVCKGGTNFTCNNKLIGARYYTPKLEGFPESAR---DNTGHGSHTASIAAG 217

Query: 181 GLVRQVSMMGLGRGTARGGVPSARIAVYKVCWSD--GCHDADILAAFDDAIADGVNIISG 240
             V+ VS  GLG GT RGGVP+ARIAVYKVC      C    ILAAFDDAIAD V+II+ 
Sbjct: 218 NAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITV 277

Query: 241 SLGASRVGDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDR 300
           SLGA  VG +  D++AIG+FHAM KGILT    GNNGP+  TIV+ APW  +VAAS  +R
Sbjct: 278 SLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNR 337

Query: 301 KFTTKVELGDGRAFDGVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELV 360
            F TKV LG+G+   G S+N+FDLNG + PLV+ G    +  D S +  C  G ++ + V
Sbjct: 338 AFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVY-GKSASSRCDASSAGFCSPGCLDSKRV 397

Query: 361 KGKIVVCDSLAVPEGVVAVEGAAGIIMQDDSPQDDTNS-YPLPASHLPPKQGALILSYIN 420
           KGKIV+CD+   P    A+   A I+    +P +D  S +  P S L      ++LSY+N
Sbjct: 398 KGKIVLCDTQRNPGEAQAMGAVASIVR---NPYEDAASVFSFPVSVLSEDDYNIVLSYVN 457

Query: 421 STNSVATATILKSTERKRKRAPI------------------PDLSGPGVEILAAWPSTVS 480
           ST +   A +LKS     ++AP+                  PD++ PG EILAA+   V 
Sbjct: 458 STKN-PKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVP 517

Query: 481 PSGAKDDNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKR 540
           PS  + D + V Y +ISGTSM+CPHV   AAY+K+FHP WSP+ ++SA+MTTA+PM+   
Sbjct: 518 PS--ESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNAST 577

Query: 541 NPA---GEFAYGAGHINPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCS 600
           +P+    EFAYGAGH++P+ A+HPGL+Y A++ D+I FLCG  YT + L+ +S D+++C+
Sbjct: 578 SPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCT 637

Query: 601 LKNSDTVF-DLNYPSFALSTPTSKPINQIYRRSVTNVGSKYATYKATIVNPSKNLEITVK 660
            + + ++  +LNYPS +     +KP    +RR+VTNVG   ATYKA +V     L++ V 
Sbjct: 638 KEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVV--GSKLKVKVV 697

Query: 661 PSVLSFKNLGEEQSFELTIKGRIGK--NIESASLVWDDGRHKVKSPITVF 681
           P+VLS K+L E++SF +T+ G   K  N+ SA L+W DG H V+SPI V+
Sbjct: 698 PAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of Lag0039317 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 587.4 bits (1513), Expect = 1.5e-167
Identity = 318/695 (45.76%), Postives = 433/695 (62.30%), Query Frame = 0

Query: 13  SHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEGVVSVFPNGIKQL 72
           S H+ +L++  G +     L+ SY+RSFNGF  +LTE+E   ++++EGVVSVFPN I QL
Sbjct: 50  SDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQL 109

Query: 73  HTTRSWDFMGFSE--QVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGPPPPNWKGSCE 132
           HTT SWDFMG  E     R  ++ES+ I+GV D+GIWPES SF DKG+GPPP  WKG C 
Sbjct: 110 HTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCS 169

Query: 133 VSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLVRQVSMMGLGR 192
              NF+CNNK+IGAR Y S G           D++GHGTHTAST AG  V+  S  G+G 
Sbjct: 170 GGKNFTCNNKLIGARDYTSEGTR---------DTSGHGTHTASTAAGNAVKDTSFFGIGN 229

Query: 193 GTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGASRVGDYFNDSI 252
           GT RGGVP++RIA YKVC   GC    +L++FDDAIADGV++I+ S+G      + +D I
Sbjct: 230 GTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPI 289

Query: 253 AIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTKVELGDGRAFD 312
           AIG+FHAM KGILT  + GN+GP  TT+ + APW  +VAASTT+R F TKV LG+G+   
Sbjct: 290 AIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLA 349

Query: 313 GVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIVVCDSLAVPEG 372
           G S+N FD+ G + PLV+      +  D   + +C    + K  VKGKI+VC     P G
Sbjct: 350 GRSVNAFDMKGKKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGG---PSG 409

Query: 373 VVAVEGAAGIIMQDDSPQDDTN-SYPLPASHLPPKQGALILSYINSTNSVATATILKSTE 432
               +    I + D SP+ D   ++ LPAS L  K    ++SYI S +S   A +LK+  
Sbjct: 410 YKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDS-PQAAVLKTET 469

Query: 433 RKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAKDDNKRVLYNI 492
              + +P+                  PD++ PGVEILAA+     PS  +DD +RV Y++
Sbjct: 470 IFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSV 529

Query: 493 ISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRN--PAGEFAYGAGHI 552
            SGTSMACPHV   AAYVK+F+P WSP+ ++SA+MTTA+P+  K     + EFAYGAGH+
Sbjct: 530 FSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHV 589

Query: 553 NPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVFDLNYPSF- 612
           +P+ A++PGL+Y   + D+I FLCG  YT++ L+ +S D   CS KN     +LNYPS  
Sbjct: 590 DPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMS 649

Query: 613 ALSTPTSKPINQIYRRSVTNVGSKYATYKATIV-NPSKNLEITVKPSVLSFKNLGEEQSF 672
           A  + T    +  + R++TNVG+  +TYK+ +V      L I V PSVL FK + E+QSF
Sbjct: 650 AKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSF 709

Query: 673 ELTIKGR-IGKNI-ESASLVWDDGRHKVKSPITVF 681
            +T+ G  +   +  SA+L+W DG H V+SPI V+
Sbjct: 710 SVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 729

BLAST of Lag0039317 vs. TAIR 10
Match: AT5G59090.3 (subtilase 4.12 )

HSP 1 Score: 582.0 bits (1499), Expect = 6.2e-166
Identity = 318/695 (45.76%), Postives = 431/695 (62.01%), Query Frame = 0

Query: 13  SHHIRLLRETIGSTFGPHSLLHSYQRSFNGFVVKLTEAEAKKVSKMEGVVSVFPNGIKQL 72
           S H+ +L++  G +     L+ SY+RSFNGF  +LTE+E   ++  EGVVSVFPN I QL
Sbjct: 50  SDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIA--EGVVSVFPNKILQL 109

Query: 73  HTTRSWDFMGFSE--QVDRVPSVESNIIVGVFDSGIWPESPSFDDKGYGPPPPNWKGSCE 132
           HTT SWDFMG  E     R  ++ES+ I+GV D+GIWPES SF DKG+GPPP  WKG C 
Sbjct: 110 HTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCS 169

Query: 133 VSANFSCNNKIIGARSYRSAGQHPEDDIRGPGDSNGHGTHTASTVAGGLVRQVSMMGLGR 192
              NF+CNNK+IGAR Y S G           D++GHGTHTAST AG  V+  S  G+G 
Sbjct: 170 GGKNFTCNNKLIGARDYTSEGTR---------DTSGHGTHTASTAAGNAVKDTSFFGIGN 229

Query: 193 GTARGGVPSARIAVYKVCWSDGCHDADILAAFDDAIADGVNIISGSLGASRVGDYFNDSI 252
           GT RGGVP++RIA YKVC   GC    +L++FDDAIADGV++I+ S+G      + +D I
Sbjct: 230 GTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPI 289

Query: 253 AIGSFHAMKKGILTSLAVGNNGPDLTTIVNFAPWSLSVAASTTDRKFTTKVELGDGRAFD 312
           AIG+FHAM KGILT  + GN+GP  TT+ + APW  +VAASTT+R F TKV LG+G+   
Sbjct: 290 AIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLA 349

Query: 313 GVSINTFDLNGTQIPLVFAGDIPKAPFDRSVSRMCFEGTVEKELVKGKIVVCDSLAVPEG 372
           G S+N FD+ G + PLV+      +  D   + +C    + K  VKGKI+VC     P G
Sbjct: 350 GRSVNAFDMKGKKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGG---PSG 409

Query: 373 VVAVEGAAGIIMQDDSPQDDTN-SYPLPASHLPPKQGALILSYINSTNSVATATILKSTE 432
               +    I + D SP+ D   ++ LPAS L  K    ++SYI S +S   A +LK+  
Sbjct: 410 YKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDS-PQAAVLKTET 469

Query: 433 RKRKRAPI------------------PDLSGPGVEILAAWPSTVSPSGAKDDNKRVLYNI 492
              + +P+                  PD++ PGVEILAA+     PS  +DD +RV Y++
Sbjct: 470 IFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSV 529

Query: 493 ISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALMTTAFPMSLKRN--PAGEFAYGAGHI 552
            SGTSMACPHV   AAYVK+F+P WSP+ ++SA+MTTA+P+  K     + EFAYGAGH+
Sbjct: 530 FSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHV 589

Query: 553 NPLGAVHPGLIYNASEIDYIKFLCGQGYTTELLQRVSEDNTTCSLKNSDTVFDLNYPSF- 612
           +P+ A++PGL+Y   + D+I FLCG  YT++ L+ +S D   CS KN     +LNYPS  
Sbjct: 590 DPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMS 649

Query: 613 ALSTPTSKPINQIYRRSVTNVGSKYATYKATIV-NPSKNLEITVKPSVLSFKNLGEEQSF 672
           A  + T    +  + R++TNVG+  +TYK+ +V      L I V PSVL FK + E+QSF
Sbjct: 650 AKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSF 709

Query: 673 ELTIKGR-IGKNI-ESASLVWDDGRHKVKSPITVF 681
            +T+ G  +   +  SA+L+W DG H V+SPI V+
Sbjct: 710 SVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 727