Lag0039303 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0039303
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAB hydrolase-1 domain-containing protein
Locationchr2: 41012956 .. 41014166 (+)
RNA-Seq ExpressionLag0039303
SyntenyLag0039303
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTTTATTCAGAGATTTATTGTTCTTGTCATGCATGTCACCGTGTCCTGATGTTTTAGACGGAGATTTCCTCAGGAATATTGCCGCATTGTCTCAGTATTCAACTGTCTATGCTATCGACCTTCTTGGCTTTGGTGCATCCGATAAACCTGCCGGCTTTGCATATACCATGGAAACATGGGCAGATGTAGGTTCAAATGGAAGTTTCAAATTTGTATTTGATCTTATTAGTTGATGACCAATTATCGATTGACAGGAACTCGCTAACATGACCCGTTTCTTATTGGTGATAGTTGATATTGGATTTTGTGGATCAAGTTATTAAGAAGCCGACTGTGCTAGTTGGTAACTCAGTGGGAAGCCTTGCTTGTGTGATTGCTGCTTCAGGTGTGTGAACCAACATTTACAGAAATAAGTCCTTAGAAAGTACTCTTGATTTGTCGATGTGAATGTATGTTTTCACATGAAACCAGATTCTAGTCGCAATCTAGTTCGAGGACTTGTGCTCTTAAACTGTGCTGGTGGTATGAACAATAAAGCAATTGTTGACGATTGGAGGATCAAACTGTTATTACCTTTGCTTTGGCTGTTTGATTTCTTATTGAACCAGCGGGGAATTGCTTCAGCGCTGTTTGAGCGTGTGAAAAAGAGGTATTTGTATTTGGTTTACTTGTAGTCAGCTTTTGTTTTTCATGTGGCTAAATGGAGGACAAAATTAGAGAAAGTGTAATACTTCTACGCAGAGATAGCTTGAGGAACATTTTGTCATCCGTATATGGAAACAAGGAAAGTGTGGATGAAGATCTGATAGACGTAGGGCAATTTCTCCCCAACTTACCTCTGTTCTTACTGGGATTAATGATGTTCTAACCGCATGTTTTGAACTGATAAATATAGATAATTGTGGAACCAGCAAATGACGAAGGGGCACTTGATGCTTTCGTCTCAATTGTAACGGGGCCACCGGGGCCGAGCCCGGTGCAGCTAATGCCGAAAATTTCCGTGCCTGTTCTTATTTTGTGGGGAGATGAAGATCCATTCACGCCACTTGATGGCCCTGTTGGAAAGTACTTTTCTAAGTTATCTATGGAACTACCGAATGTCAGTCTTTTTGTGTTGAAAGGAGTAGGACATTGCCCTCATGATGATAGGCCTGAATTAGTGCACGAAAAGCTCCTTCCATGGCTGGCTCAGCTTTCTGCTTTGTAA

mRNA sequence

ATGCCTTTATTCAGAGATTTATTGTTCTTGTCATGCATGTCACCGTGTCCTGATGTTTTAGACGGAGATTTCCTCAGGAATATTGCCGCATTGTCTCAGTATTCAACTGTCTATGCTATCGACCTTCTTGGCTTTGGTGCATCCGATAAACCTGCCGGCTTTGCATATACCATGGAAACATGGGCAGATTTGATATTGGATTTTGTGGATCAAGTTATTAAGAAGCCGACTGTGCTAGTTGGTAACTCAGTGGGAAGCCTTGCTTGTGTGATTGCTGCTTCAGATTCTAGTCGCAATCTAGTTCGAGGACTTGTGCTCTTAAACTGTGCTGGTGGTATGAACAATAAAGCAATTGTTGACGATTGGAGGATCAAACTGTTATTACCTTTGCTTTGGCTGTTTGATTTCTTATTGAACCAGCGGGGAATTGCTTCAGCGCTGTTTGAGCGTGTGAAAAAGAGAGATAGCTTGAGGAACATTTTGTCATCCGTATATGGAAACAAGGAAAGTGTGGATGAAGATCTGATAGACATAATTGTGGAACCAGCAAATGACGAAGGGGCACTTGATGCTTTCGTCTCAATTGTAACGGGGCCACCGGGGCCGAGCCCGGTGCAGCTAATGCCGAAAATTTCCGTGCCTGTTCTTATTTTGTGGGGAGATGAAGATCCATTCACGCCACTTGATGGCCCTGTTGGAAAGTACTTTTCTAAGTTATCTATGGAACTACCGAATGTCAGTCTTTTTGTGTTGAAAGGAGTAGGACATTGCCCTCATGATGATAGGCCTGAATTAGTGCACGAAAAGCTCCTTCCATGGCTGGCTCAGCTTTCTGCTTTGTAA

Coding sequence (CDS)

ATGCCTTTATTCAGAGATTTATTGTTCTTGTCATGCATGTCACCGTGTCCTGATGTTTTAGACGGAGATTTCCTCAGGAATATTGCCGCATTGTCTCAGTATTCAACTGTCTATGCTATCGACCTTCTTGGCTTTGGTGCATCCGATAAACCTGCCGGCTTTGCATATACCATGGAAACATGGGCAGATTTGATATTGGATTTTGTGGATCAAGTTATTAAGAAGCCGACTGTGCTAGTTGGTAACTCAGTGGGAAGCCTTGCTTGTGTGATTGCTGCTTCAGATTCTAGTCGCAATCTAGTTCGAGGACTTGTGCTCTTAAACTGTGCTGGTGGTATGAACAATAAAGCAATTGTTGACGATTGGAGGATCAAACTGTTATTACCTTTGCTTTGGCTGTTTGATTTCTTATTGAACCAGCGGGGAATTGCTTCAGCGCTGTTTGAGCGTGTGAAAAAGAGAGATAGCTTGAGGAACATTTTGTCATCCGTATATGGAAACAAGGAAAGTGTGGATGAAGATCTGATAGACATAATTGTGGAACCAGCAAATGACGAAGGGGCACTTGATGCTTTCGTCTCAATTGTAACGGGGCCACCGGGGCCGAGCCCGGTGCAGCTAATGCCGAAAATTTCCGTGCCTGTTCTTATTTTGTGGGGAGATGAAGATCCATTCACGCCACTTGATGGCCCTGTTGGAAAGTACTTTTCTAAGTTATCTATGGAACTACCGAATGTCAGTCTTTTTGTGTTGAAAGGAGTAGGACATTGCCCTCATGATGATAGGCCTGAATTAGTGCACGAAAAGCTCCTTCCATGGCTGGCTCAGCTTTCTGCTTTGTAA

Protein sequence

MPLFRDLLFLSCMSPCPDVLDGDFLRNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMETWADLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLNQRGIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLSMELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWLAQLSAL
Homology
BLAST of Lag0039303 vs. NCBI nr
Match: XP_023523833.1 (pheophytinase, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 531.9 bits (1369), Expect = 3.2e-147
Identity = 264/280 (94.29%), Postives = 270/280 (96.43%), Query Frame = 0

Query: 1   MPLFRDLLFLSCMSPCPDVLDGDFLRNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMET 60
           MPLFRDLLFL+CMSP PDVL GDF RNI+ALSQYSTVYAIDLLGFGASDKPAGFAYTMET
Sbjct: 69  MPLFRDLLFLTCMSPGPDVLFGDFFRNISALSQYSTVYAIDLLGFGASDKPAGFAYTMET 128

Query: 61  WADLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 120
           WADLILDFV  +IK+PTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD
Sbjct: 129 WADLILDFVKDIIKRPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 188

Query: 121 DWRIKLLLPLLWLFDFLLNQRGIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIV 180
           DWRIKLLLPLLWL DFLLNQR IASALFERVKKRDSLRNILSSVYGNKE+VDEDLIDII+
Sbjct: 189 DWRIKLLLPLLWLVDFLLNQRRIASALFERVKKRDSLRNILSSVYGNKENVDEDLIDIIL 248

Query: 181 EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS 240
           EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS
Sbjct: 249 EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS 308

Query: 241 MELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWLAQLSAL 281
           ME PNVSL VLKGVGHCPHDDRPELVHEKLLPWLAQL AL
Sbjct: 309 MEAPNVSLCVLKGVGHCPHDDRPELVHEKLLPWLAQLHAL 348

BLAST of Lag0039303 vs. NCBI nr
Match: KAE8646581.1 (hypothetical protein Csa_005070 [Cucumis sativus])

HSP 1 Score: 528.5 bits (1360), Expect = 3.6e-146
Identity = 262/280 (93.57%), Postives = 268/280 (95.71%), Query Frame = 0

Query: 1   MPLFRDLLFLSCMSPCPDVLDGDFLRNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMET 60
           MPL RD LF+SCMS  PD+L GD  RNI+ LSQYSTVYAIDLLGFGASDKPAGFAYTMET
Sbjct: 1   MPLLRDFLFMSCMSASPDILFGDVFRNISTLSQYSTVYAIDLLGFGASDKPAGFAYTMET 60

Query: 61  WADLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 120
           WA+LILDFVDQVIKKPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD
Sbjct: 61  WAELILDFVDQVIKKPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 120

Query: 121 DWRIKLLLPLLWLFDFLLNQRGIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIV 180
           DWRIKLLLPLLWLFDFLLNQR IASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIV
Sbjct: 121 DWRIKLLLPLLWLFDFLLNQRIIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIV 180

Query: 181 EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS 240
           EPANDEGALDAFVSIVTGPPGPSPV+LMPKISVPVLILWGDEDPFTPLDGPVGKYF KLS
Sbjct: 181 EPANDEGALDAFVSIVTGPPGPSPVELMPKISVPVLILWGDEDPFTPLDGPVGKYFKKLS 240

Query: 241 MELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWLAQLSAL 281
           ME+ NVSLFVLKGVGHCPHDDRPELVHEKLLPWLAQL AL
Sbjct: 241 MEVSNVSLFVLKGVGHCPHDDRPELVHEKLLPWLAQLPAL 280

BLAST of Lag0039303 vs. NCBI nr
Match: XP_022973446.1 (pheophytinase, chloroplastic isoform X3 [Cucurbita maxima])

HSP 1 Score: 527.3 bits (1357), Expect = 7.9e-146
Identity = 262/280 (93.57%), Postives = 269/280 (96.07%), Query Frame = 0

Query: 1   MPLFRDLLFLSCMSPCPDVLDGDFLRNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMET 60
           MPLFRDLLFL+CMSP PDVL GDF RNI+ALSQYSTVYAIDLLGFGASDKPAGFAYTMET
Sbjct: 106 MPLFRDLLFLTCMSPGPDVLFGDFFRNISALSQYSTVYAIDLLGFGASDKPAGFAYTMET 165

Query: 61  WADLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 120
           WADLILDFV  +IK+PTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD
Sbjct: 166 WADLILDFVKDIIKRPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 225

Query: 121 DWRIKLLLPLLWLFDFLLNQRGIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIV 180
           DWRIKLLLPLLWL DFLLNQR IASALFERVKKRDSLR+ILSSVYGNKE+VDEDLIDII 
Sbjct: 226 DWRIKLLLPLLWLVDFLLNQRRIASALFERVKKRDSLRDILSSVYGNKENVDEDLIDIIF 285

Query: 181 EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS 240
           EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS
Sbjct: 286 EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS 345

Query: 241 MELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWLAQLSAL 281
           +E PNVSL VLKGVGHCPHDDRPELVHEKLLPWLAQL AL
Sbjct: 346 VESPNVSLCVLKGVGHCPHDDRPELVHEKLLPWLAQLHAL 385

BLAST of Lag0039303 vs. NCBI nr
Match: XP_022973444.1 (pheophytinase, chloroplastic isoform X2 [Cucurbita maxima] >XP_022973445.1 pheophytinase, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 527.3 bits (1357), Expect = 7.9e-146
Identity = 262/280 (93.57%), Postives = 269/280 (96.07%), Query Frame = 0

Query: 1   MPLFRDLLFLSCMSPCPDVLDGDFLRNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMET 60
           MPLFRDLLFL+CMSP PDVL GDF RNI+ALSQYSTVYAIDLLGFGASDKPAGFAYTMET
Sbjct: 116 MPLFRDLLFLTCMSPGPDVLFGDFFRNISALSQYSTVYAIDLLGFGASDKPAGFAYTMET 175

Query: 61  WADLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 120
           WADLILDFV  +IK+PTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD
Sbjct: 176 WADLILDFVKDIIKRPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 235

Query: 121 DWRIKLLLPLLWLFDFLLNQRGIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIV 180
           DWRIKLLLPLLWL DFLLNQR IASALFERVKKRDSLR+ILSSVYGNKE+VDEDLIDII 
Sbjct: 236 DWRIKLLLPLLWLVDFLLNQRRIASALFERVKKRDSLRDILSSVYGNKENVDEDLIDIIF 295

Query: 181 EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS 240
           EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS
Sbjct: 296 EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS 355

Query: 241 MELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWLAQLSAL 281
           +E PNVSL VLKGVGHCPHDDRPELVHEKLLPWLAQL AL
Sbjct: 356 VESPNVSLCVLKGVGHCPHDDRPELVHEKLLPWLAQLHAL 395

BLAST of Lag0039303 vs. NCBI nr
Match: XP_038884602.1 (pheophytinase, chloroplastic isoform X4 [Benincasa hispida])

HSP 1 Score: 526.9 bits (1356), Expect = 1.0e-145
Identity = 264/280 (94.29%), Postives = 269/280 (96.07%), Query Frame = 0

Query: 1   MPLFRDLLFLSCMSPCPDVLDGDFLRNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMET 60
           MPLFRD LF+SCMS  PD+L GDF RNI+ LSQYSTVYAIDLLGFGAS+KPAGFAYTMET
Sbjct: 26  MPLFRD-LFMSCMSARPDILFGDFFRNISTLSQYSTVYAIDLLGFGASNKPAGFAYTMET 85

Query: 61  WADLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 120
           WADLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD
Sbjct: 86  WADLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 145

Query: 121 DWRIKLLLPLLWLFDFLLNQRGIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIV 180
           DWRIKLLLPLLWLFDFLLNQR IASALFERVKKRDSLRNIL SVYGNKESVDEDLIDIIV
Sbjct: 146 DWRIKLLLPLLWLFDFLLNQRVIASALFERVKKRDSLRNILLSVYGNKESVDEDLIDIIV 205

Query: 181 EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS 240
           EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS
Sbjct: 206 EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS 265

Query: 241 MELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWLAQLSAL 281
            E+PNVSLFVLKGVGHCPHDDRPE VHEKLLPWLAQL AL
Sbjct: 266 KEVPNVSLFVLKGVGHCPHDDRPESVHEKLLPWLAQLPAL 304

BLAST of Lag0039303 vs. ExPASy Swiss-Prot
Match: Q9FFZ1 (Pheophytinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPH PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 3.8e-18
Identity = 66/246 (26.83%), Postives = 116/246 (47.15%), Query Frame = 0

Query: 44  GFGASDKPAG--FAYTMETWADLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSSRNLV 103
           GFG   +P      ++++ W D +  FV++VI +P  + GNS+G    +  A+ +  +LV
Sbjct: 180 GFGDKTEPWADQLVFSLDLWRDQVQYFVEEVIGEPVYIAGNSLGGYVALYFAA-THPHLV 239

Query: 104 RGLVLLNCA---GGMNNKAIVDDWRIKLLLPLLWLFDFLLNQRGIASALFERVKKRDSLR 163
           +G+ LLN     G   N   V   ++  L P    F      + I   +++++   +S+ 
Sbjct: 240 KGVTLLNATPFWGFFPNP--VRSPKLARLFPWPGAFPLPERVKKITELVWQKISDPESIA 299

Query: 164 NILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPG----PSPVQLMPKISVP 223
            IL  VY +     + +   IVE      A  +F SI+  P G       +    + +V 
Sbjct: 300 EILKQVYTDHSINVDKVFSRIVEVTQHPAAAASFASIMLAPGGELSFSEALSRCKENNVQ 359

Query: 224 VLILWGDEDPFT-PLDGPVGKYFSKLSMELPNVSLFVLKGVGHCPHDDRPELVHEKLLPW 280
           + +++G EDP+  PL G       K+  E+PN   + +   GHCPHD+ PE+V+  +  W
Sbjct: 360 ICLMYGREDPWVRPLWG------KKIKKEIPNAPYYEISPAGHCPHDEVPEVVNYLMRGW 416

BLAST of Lag0039303 vs. ExPASy Swiss-Prot
Match: B2JQW2 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) OX=391038 GN=mhpC PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 9.8e-06
Identity = 62/258 (24.03%), Postives = 106/258 (41.09%), Query Frame = 0

Query: 23  DFLRNIAALSQYS-TVYAIDLLGFGASDKPAGFAYTMETWADLILDFVDQVIKKPTVLVG 82
           +F RN+ A +     V  +D  G+G SD         +  A ++   +D +  +   LVG
Sbjct: 54  NFYRNVDAFANAGYRVILVDCPGWGKSDSIVCTGSRSDLNARVLKGVLDTLGIERAHLVG 113

Query: 83  NSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLNQR 142
           NS+G  + V  A      + + +++    GG +    +    IKLL  L           
Sbjct: 114 NSMGGHSAVAFALSYPERVGKLVLMGGGTGGPSQFVPMPTEGIKLLQGLY---------- 173

Query: 143 GIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVE-PANDEGALDAFVSIVTGPP 202
                   R    ++L+ +L+    +  ++ E+L+   ++        L+ FV  +T  P
Sbjct: 174 --------RDPTLENLKKMLNVFVYDASTMTEELMQTRLDNMLARRDHLENFVKSLTANP 233

Query: 203 G--PSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLSMELPNVSLFVLKGVGHCP 262
              P     + +I  P L++WG +D F PLD  VG    +L   LPN    V    GH  
Sbjct: 234 KQFPDYGHRLNEIKAPALVIWGRDDRFVPLD--VG---LRLVWGLPNAEFHVFGRCGHWA 288

Query: 263 HDDRPELVHEKLLPWLAQ 277
             +  E  ++ LL +L Q
Sbjct: 294 QWEHAERFNQMLLDFLGQ 288

BLAST of Lag0039303 vs. ExPASy Swiss-Prot
Match: P9WNH4 (4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=hsaD PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 2.2e-05
Identity = 61/240 (25.42%), Postives = 93/240 (38.75%), Query Frame = 0

Query: 23  DFLRNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMETWADLILDFVDQVIKKPTVLVGN 82
           +F RNIA L+++  V A+D  G+G SDK A         A  +    DQ+      LVGN
Sbjct: 54  NFSRNIAVLARHFHVLAVDQPGYGHSDKRAEHGQFNRYAAMALKGLFDQLGLGRVPLVGN 113

Query: 83  SVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLNQRG 142
           S+G    V  A D       G ++L   GG++      D                    G
Sbjct: 114 SLGGGTAVRFALDYPAR--AGRLVLMGPGGLSINLFAPD-----------------PTEG 173

Query: 143 IAS-ALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPG 202
           +   + F     R++L   L  +  +K  +  +L+D     A+   +L A  ++     G
Sbjct: 174 VKRLSKFSVAPTRENLEAFLRVMVYDKNLITPELVDQRFALASTPESLTATRAMGKSFAG 233

Query: 203 PSPVQLMP-----KISVPVLILWGDEDPFTPLDGPVGKYFSKLSMELPNVSLFVLKGVGH 257
                 M      ++  PVL++WG ED   PLDG +          +P   L V    GH
Sbjct: 234 ADFEAGMMWREVYRLRQPVLLIWGREDRVNPLDGAL-----VALKTIPRAQLHVFGQCGH 269

BLAST of Lag0039303 vs. ExPASy Swiss-Prot
Match: P9WNH5 (4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=hsaD PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 2.2e-05
Identity = 61/240 (25.42%), Postives = 93/240 (38.75%), Query Frame = 0

Query: 23  DFLRNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMETWADLILDFVDQVIKKPTVLVGN 82
           +F RNIA L+++  V A+D  G+G SDK A         A  +    DQ+      LVGN
Sbjct: 54  NFSRNIAVLARHFHVLAVDQPGYGHSDKRAEHGQFNRYAAMALKGLFDQLGLGRVPLVGN 113

Query: 83  SVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLNQRG 142
           S+G    V  A D       G ++L   GG++      D                    G
Sbjct: 114 SLGGGTAVRFALDYPAR--AGRLVLMGPGGLSINLFAPD-----------------PTEG 173

Query: 143 IAS-ALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPG 202
           +   + F     R++L   L  +  +K  +  +L+D     A+   +L A  ++     G
Sbjct: 174 VKRLSKFSVAPTRENLEAFLRVMVYDKNLITPELVDQRFALASTPESLTATRAMGKSFAG 233

Query: 203 PSPVQLMP-----KISVPVLILWGDEDPFTPLDGPVGKYFSKLSMELPNVSLFVLKGVGH 257
                 M      ++  PVL++WG ED   PLDG +          +P   L V    GH
Sbjct: 234 ADFEAGMMWREVYRLRQPVLLIWGREDRVNPLDGAL-----VALKTIPRAQLHVFGQCGH 269

BLAST of Lag0039303 vs. ExPASy Swiss-Prot
Match: A4JPX5 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) OX=269482 GN=mhpC PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.8e-05
Identity = 61/256 (23.83%), Postives = 106/256 (41.41%), Query Frame = 0

Query: 23  DFLRNIAALSQYS-TVYAIDLLGFGASDKPAGFAYTMETWADLILDFVDQVIKKPTVLVG 82
           +F RN+ A +     V  +D  G+G SD         +  A ++   +D +      LVG
Sbjct: 54  NFHRNVDAFANAGYRVILVDCPGWGKSDSIVCTGSRSDLNARVLAGVLDTLGIGRAHLVG 113

Query: 83  NSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLNQR 142
           NS+G  + V  A      + + +++    GG +    +    IKLL              
Sbjct: 114 NSMGGHSAVAFALSYPERVGKLVLMGGGTGGPSQFVPMPTEGIKLL-------------- 173

Query: 143 GIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVE-PANDEGALDAFVSIVTGPP 202
               AL+ R    ++L+ +L+    +  ++ E+L+   +E        L+ FV  +T  P
Sbjct: 174 ---QALY-RDPTLENLKKMLNVFVYDASTMTEELMQTRLENMLGRRDHLENFVKSLTANP 233

Query: 203 G--PSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLSMELPNVSLFVLKGVGHCP 262
              P     + +I  P L++WG +D F P+D  VG    +L   +PN  L V    GH  
Sbjct: 234 KQFPDYGHRLSEIKAPALVIWGRDDRFVPMD--VG---LRLVWNMPNADLHVFGRCGHWA 286

Query: 263 HDDRPELVHEKLLPWL 275
             +  E  +  +L +L
Sbjct: 294 QWEHAERFNRMVLEFL 286

BLAST of Lag0039303 vs. ExPASy TrEMBL
Match: A0A6J1IBD4 (pheophytinase, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111471988 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 3.8e-146
Identity = 262/280 (93.57%), Postives = 269/280 (96.07%), Query Frame = 0

Query: 1   MPLFRDLLFLSCMSPCPDVLDGDFLRNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMET 60
           MPLFRDLLFL+CMSP PDVL GDF RNI+ALSQYSTVYAIDLLGFGASDKPAGFAYTMET
Sbjct: 116 MPLFRDLLFLTCMSPGPDVLFGDFFRNISALSQYSTVYAIDLLGFGASDKPAGFAYTMET 175

Query: 61  WADLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 120
           WADLILDFV  +IK+PTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD
Sbjct: 176 WADLILDFVKDIIKRPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 235

Query: 121 DWRIKLLLPLLWLFDFLLNQRGIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIV 180
           DWRIKLLLPLLWL DFLLNQR IASALFERVKKRDSLR+ILSSVYGNKE+VDEDLIDII 
Sbjct: 236 DWRIKLLLPLLWLVDFLLNQRRIASALFERVKKRDSLRDILSSVYGNKENVDEDLIDIIF 295

Query: 181 EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS 240
           EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS
Sbjct: 296 EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS 355

Query: 241 MELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWLAQLSAL 281
           +E PNVSL VLKGVGHCPHDDRPELVHEKLLPWLAQL AL
Sbjct: 356 VESPNVSLCVLKGVGHCPHDDRPELVHEKLLPWLAQLHAL 395

BLAST of Lag0039303 vs. ExPASy TrEMBL
Match: A0A6J1I8N7 (pheophytinase, chloroplastic isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111471988 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 3.8e-146
Identity = 262/280 (93.57%), Postives = 269/280 (96.07%), Query Frame = 0

Query: 1   MPLFRDLLFLSCMSPCPDVLDGDFLRNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMET 60
           MPLFRDLLFL+CMSP PDVL GDF RNI+ALSQYSTVYAIDLLGFGASDKPAGFAYTMET
Sbjct: 106 MPLFRDLLFLTCMSPGPDVLFGDFFRNISALSQYSTVYAIDLLGFGASDKPAGFAYTMET 165

Query: 61  WADLILDFVDQVIKKPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 120
           WADLILDFV  +IK+PTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD
Sbjct: 166 WADLILDFVKDIIKRPTVLVGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVD 225

Query: 121 DWRIKLLLPLLWLFDFLLNQRGIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIV 180
           DWRIKLLLPLLWL DFLLNQR IASALFERVKKRDSLR+ILSSVYGNKE+VDEDLIDII 
Sbjct: 226 DWRIKLLLPLLWLVDFLLNQRRIASALFERVKKRDSLRDILSSVYGNKENVDEDLIDIIF 285

Query: 181 EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS 240
           EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS
Sbjct: 286 EPANDEGALDAFVSIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLS 345

Query: 241 MELPNVSLFVLKGVGHCPHDDRPELVHEKLLPWLAQLSAL 281
           +E PNVSL VLKGVGHCPHDDRPELVHEKLLPWLAQL AL
Sbjct: 346 VESPNVSLCVLKGVGHCPHDDRPELVHEKLLPWLAQLHAL 385

BLAST of Lag0039303 vs. ExPASy TrEMBL
Match: A0A0A0KAI8 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014690 PE=4 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.6e-136
Identity = 246/255 (96.47%), Postives = 250/255 (98.04%), Query Frame = 0

Query: 26  RNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMETWADLILDFVDQVIKKPTVLVGNSVG 85
           RNI+ LSQYSTVYAIDLLGFGASDKPAGFAYTMETWA+LILDFVDQVIKKPTVLVGNSVG
Sbjct: 175 RNISTLSQYSTVYAIDLLGFGASDKPAGFAYTMETWAELILDFVDQVIKKPTVLVGNSVG 234

Query: 86  SLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLNQRGIAS 145
           SLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLNQR IAS
Sbjct: 235 SLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLNQRIIAS 294

Query: 146 ALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPV 205
           ALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPV
Sbjct: 295 ALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPV 354

Query: 206 QLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLSMELPNVSLFVLKGVGHCPHDDRPEL 265
           +LMPKISVPVLILWGDEDPFTPLDGPVGKYF KLSME+ NVSLFVLKGVGHCPHDDRPEL
Sbjct: 355 ELMPKISVPVLILWGDEDPFTPLDGPVGKYFKKLSMEVSNVSLFVLKGVGHCPHDDRPEL 414

Query: 266 VHEKLLPWLAQLSAL 281
           VHEKLLPWLAQL AL
Sbjct: 415 VHEKLLPWLAQLPAL 429

BLAST of Lag0039303 vs. ExPASy TrEMBL
Match: A0A5A7U2I7 (Pheophytinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold155G001000 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 6.8e-135
Identity = 240/255 (94.12%), Postives = 250/255 (98.04%), Query Frame = 0

Query: 26  RNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMETWADLILDFVDQVIKKPTVLVGNSVG 85
           RNI+ LSQYSTVYAIDLLGFGASDKPAGFAYTMETWA+L++DFVDQ+IKKPTVLVGNSVG
Sbjct: 124 RNISTLSQYSTVYAIDLLGFGASDKPAGFAYTMETWAELVIDFVDQIIKKPTVLVGNSVG 183

Query: 86  SLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLNQRGIAS 145
           SLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWL DFLLNQR IAS
Sbjct: 184 SLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLVDFLLNQRIIAS 243

Query: 146 ALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPV 205
           ALF+RVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPV
Sbjct: 244 ALFKRVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPV 303

Query: 206 QLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLSMELPNVSLFVLKGVGHCPHDDRPEL 265
           +LMPK+SVPVLILWGD+DPFTPLDGPVGKYF KLSME+PNVSLFVLKGVGHCPHDDRPEL
Sbjct: 304 ELMPKLSVPVLILWGDKDPFTPLDGPVGKYFRKLSMEVPNVSLFVLKGVGHCPHDDRPEL 363

Query: 266 VHEKLLPWLAQLSAL 281
           VHEKLLPWLAQL AL
Sbjct: 364 VHEKLLPWLAQLPAL 378

BLAST of Lag0039303 vs. ExPASy TrEMBL
Match: A0A1S3CIV2 (pheophytinase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501331 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 6.8e-135
Identity = 240/255 (94.12%), Postives = 250/255 (98.04%), Query Frame = 0

Query: 26  RNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMETWADLILDFVDQVIKKPTVLVGNSVG 85
           RNI+ LSQYSTVYAIDLLGFGASDKPAGFAYTMETWA+L++DFVDQ+IKKPTVLVGNSVG
Sbjct: 173 RNISTLSQYSTVYAIDLLGFGASDKPAGFAYTMETWAELVIDFVDQIIKKPTVLVGNSVG 232

Query: 86  SLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLNQRGIAS 145
           SLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWL DFLLNQR IAS
Sbjct: 233 SLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLVDFLLNQRIIAS 292

Query: 146 ALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPV 205
           ALF+RVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPV
Sbjct: 293 ALFKRVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPV 352

Query: 206 QLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLSMELPNVSLFVLKGVGHCPHDDRPEL 265
           +LMPK+SVPVLILWGD+DPFTPLDGPVGKYF KLSME+PNVSLFVLKGVGHCPHDDRPEL
Sbjct: 353 ELMPKLSVPVLILWGDKDPFTPLDGPVGKYFRKLSMEVPNVSLFVLKGVGHCPHDDRPEL 412

Query: 266 VHEKLLPWLAQLSAL 281
           VHEKLLPWLAQL AL
Sbjct: 413 VHEKLLPWLAQLPAL 427

BLAST of Lag0039303 vs. TAIR 10
Match: AT5G38520.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 399.8 bits (1026), Expect = 1.8e-111
Identity = 186/254 (73.23%), Postives = 230/254 (90.55%), Query Frame = 0

Query: 26  RNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMETWADLILDFVDQVIKKPTVLVGNSVG 85
           RNI ALS+  TVYAIDLLGFGASDKP GF+YTME+WA+LIL+F+++V++KPT+L+GNSVG
Sbjct: 108 RNINALSKNHTVYAIDLLGFGASDKPPGFSYTMESWAELILNFLEEVVQKPTILIGNSVG 167

Query: 86  SLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLNQRGIAS 145
           SLACVIAAS+S  +LV+GLVLLNCAGGMNNKA+ DDWRIKLL+PLL L DFLL QRGIAS
Sbjct: 168 SLACVIAASESRGDLVKGLVLLNCAGGMNNKAVFDDWRIKLLMPLLLLIDFLLKQRGIAS 227

Query: 146 ALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGPPGPSPV 205
           ALF RVK R++L+NIL++VYGNK++VD+ L++II  PAN EGALDAFVSI+TGPPGP+P+
Sbjct: 228 ALFNRVKDRENLKNILTNVYGNKDNVDDTLVEIIAGPANTEGALDAFVSILTGPPGPNPI 287

Query: 206 QLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLSMELPNVSLFVLKGVGHCPHDDRPEL 265
           +L+P+I+ PVL+LWGD+D  TPLDGPVGKYF+ L  +LPN +L+VL+GVGHCP DDRP+L
Sbjct: 288 KLIPEITKPVLVLWGDQDGLTPLDGPVGKYFTSLPDQLPNFNLYVLQGVGHCPQDDRPDL 347

Query: 266 VHEKLLPWLAQLSA 280
           VHE+LLPWLAQLS+
Sbjct: 348 VHERLLPWLAQLSS 361

BLAST of Lag0039303 vs. TAIR 10
Match: AT5G38520.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 391.0 bits (1003), Expect = 8.2e-109
Identity = 186/266 (69.92%), Postives = 230/266 (86.47%), Query Frame = 0

Query: 26  RNIAALSQYSTVYAIDLLGFGASDKPAGFAYTMETWADLILDFVDQVIKKPTVLVGNSVG 85
           RNI ALS+  TVYAIDLLGFGASDKP GF+YTME+WA+LIL+F+++V++KPT+L+GNSVG
Sbjct: 108 RNINALSKNHTVYAIDLLGFGASDKPPGFSYTMESWAELILNFLEEVVQKPTILIGNSVG 167

Query: 86  SLACVIAAS------------DSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWL 145
           SLACVIAAS            +S  +LV+GLVLLNCAGGMNNKA+ DDWRIKLL+PLL L
Sbjct: 168 SLACVIAASGTKFLIYLEKKTESRGDLVKGLVLLNCAGGMNNKAVFDDWRIKLLMPLLLL 227

Query: 146 FDFLLNQRGIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFV 205
            DFLL QRGIASALF RVK R++L+NIL++VYGNK++VD+ L++II  PAN EGALDAFV
Sbjct: 228 IDFLLKQRGIASALFNRVKDRENLKNILTNVYGNKDNVDDTLVEIIAGPANTEGALDAFV 287

Query: 206 SIVTGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLSMELPNVSLFVLKG 265
           SI+TGPPGP+P++L+P+I+ PVL+LWGD+D  TPLDGPVGKYF+ L  +LPN +L+VL+G
Sbjct: 288 SILTGPPGPNPIKLIPEITKPVLVLWGDQDGLTPLDGPVGKYFTSLPDQLPNFNLYVLQG 347

Query: 266 VGHCPHDDRPELVHEKLLPWLAQLSA 280
           VGHCP DDRP+LVHE+LLPWLAQLS+
Sbjct: 348 VGHCPQDDRPDLVHERLLPWLAQLSS 373

BLAST of Lag0039303 vs. TAIR 10
Match: AT5G19850.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 133.3 bits (334), Expect = 3.1e-31
Identity = 78/257 (30.35%), Postives = 135/257 (52.53%), Query Frame = 0

Query: 26  RNIAALSQYSTVYAIDLLGFGASDKP------AGFAYTMETWADLILDFVDQVIKKPTVL 85
           +N   L +   VY+IDL+G+G SDKP          YT ETW + + DF   V+K     
Sbjct: 112 KNTPILGKTHRVYSIDLIGYGYSDKPNPREFGGEPFYTFETWGEQLNDFCLDVVKDEAFF 171

Query: 86  VGNSVGSLACVIAASDSSRNLVRGLVLLNCAGGMNNKAIVDDWRIKLLLPLLWLFDFLLN 145
           + NS+G L  + AA  S   + RGL+L+N    ++ + +    +  +  P +  F  LL 
Sbjct: 172 ICNSIGGLVGLQAAV-SKPEICRGLMLIN----ISLRMLHIKKQPFIGRPFIKSFQNLLR 231

Query: 146 QRGIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSIVTGP 205
              +    F+ + K +++++IL   Y +   V ++L++ I+ P  + GA+D F+  +   
Sbjct: 232 NTPVGKLFFKSIAKPETVKSILCQCYHDSSQVTDELVEAILRPGLEPGAVDVFLEFICYS 291

Query: 206 PGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLSMELPNVSLFVLKGVGHCPH 265
            GP P  L+P +  PVLI WG++DP+ P++  +G+ +S            VL   GHCP 
Sbjct: 292 GGPLPEDLLPLVKCPVLIAWGEKDPWEPIE--LGRAYSNFDAV---EDFVVLPDAGHCPQ 351

Query: 266 DDRPELVHEKLLPWLAQ 277
           D++PE+V+  +  ++A+
Sbjct: 352 DEKPEMVNPLIESFVAR 358

BLAST of Lag0039303 vs. TAIR 10
Match: AT4G36530.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 129.0 bits (323), Expect = 5.8e-30
Identity = 89/259 (34.36%), Postives = 138/259 (53.28%), Query Frame = 0

Query: 27  NIAALSQYSTVYAIDLLGFGASDKPAGFAYTMETWADLILDFVDQVIKKPTVLVGNSVGS 86
           NI  L++   VYA+DLLGFG SDK A   Y    W D ++DF+ +V+K+P V+VGNS+G 
Sbjct: 61  NIPELAKKYKVYALDLLGFGWSDK-ALIEYDAMVWTDQVIDFMKEVVKEPAVVVGNSLGG 120

Query: 87  LACVIAASDSSRNLVRGLVLLNCAGGM----NNKAIVDDWRIK--LLLPLLWLFDFLLNQ 146
              +  A       V G+ LLN AG        +   D+  I   ++ PL  +F     Q
Sbjct: 121 FTALSVAVGLPEQ-VTGVALLNSAGQFAAESRKREEADETVITKFIVKPLKEIF-----Q 180

Query: 147 RGIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSI----V 206
           R +   LF + K+   + ++L SVY +  +VD+ L++ I +PA D  A + +  +    +
Sbjct: 181 RVVLGFLFWQAKQPSRIESVLKSVYIDSTNVDDYLVESISKPATDPNAGEVYYRLMTRFL 240

Query: 207 TGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLSMELPNVSLFVLKGVGH 266
           T     +   ++ K++ P+L++WGD DP+    GP      K+     N SL  L+  GH
Sbjct: 241 TNQSRYTLDSVLSKMTCPLLLVWGDLDPWV---GPAKA--EKIKAFYSNSSLVHLQ-AGH 300

Query: 267 CPHDDRPELVHEKLLPWLA 276
           CPHD+ PE V++ LL WL+
Sbjct: 301 CPHDEVPEAVNKALLDWLS 306

BLAST of Lag0039303 vs. TAIR 10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 129.0 bits (323), Expect = 5.8e-30
Identity = 89/259 (34.36%), Postives = 138/259 (53.28%), Query Frame = 0

Query: 27  NIAALSQYSTVYAIDLLGFGASDKPAGFAYTMETWADLILDFVDQVIKKPTVLVGNSVGS 86
           NI  L++   VYA+DLLGFG SDK A   Y    W D ++DF+ +V+K+P V+VGNS+G 
Sbjct: 118 NIPELAKKYKVYALDLLGFGWSDK-ALIEYDAMVWTDQVIDFMKEVVKEPAVVVGNSLGG 177

Query: 87  LACVIAASDSSRNLVRGLVLLNCAGGM----NNKAIVDDWRIK--LLLPLLWLFDFLLNQ 146
              +  A       V G+ LLN AG        +   D+  I   ++ PL  +F     Q
Sbjct: 178 FTALSVAVGLPEQ-VTGVALLNSAGQFAAESRKREEADETVITKFIVKPLKEIF-----Q 237

Query: 147 RGIASALFERVKKRDSLRNILSSVYGNKESVDEDLIDIIVEPANDEGALDAFVSI----V 206
           R +   LF + K+   + ++L SVY +  +VD+ L++ I +PA D  A + +  +    +
Sbjct: 238 RVVLGFLFWQAKQPSRIESVLKSVYIDSTNVDDYLVESISKPATDPNAGEVYYRLMTRFL 297

Query: 207 TGPPGPSPVQLMPKISVPVLILWGDEDPFTPLDGPVGKYFSKLSMELPNVSLFVLKGVGH 266
           T     +   ++ K++ P+L++WGD DP+    GP      K+     N SL  L+  GH
Sbjct: 298 TNQSRYTLDSVLSKMTCPLLLVWGDLDPWV---GPAKA--EKIKAFYSNSSLVHLQ-AGH 357

Query: 267 CPHDDRPELVHEKLLPWLA 276
           CPHD+ PE V++ LL WL+
Sbjct: 358 CPHDEVPEAVNKALLDWLS 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023523833.13.2e-14794.29pheophytinase, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo][more]
KAE8646581.13.6e-14693.57hypothetical protein Csa_005070 [Cucumis sativus][more]
XP_022973446.17.9e-14693.57pheophytinase, chloroplastic isoform X3 [Cucurbita maxima][more]
XP_022973444.17.9e-14693.57pheophytinase, chloroplastic isoform X2 [Cucurbita maxima] >XP_022973445.1 pheop... [more]
XP_038884602.11.0e-14594.29pheophytinase, chloroplastic isoform X4 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FFZ13.8e-1826.83Pheophytinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPH PE=1 SV=1[more]
B2JQW29.8e-0624.032-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Para... [more]
P9WNH42.2e-0525.424,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase O... [more]
P9WNH52.2e-0525.424,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase O... [more]
A4JPX52.8e-0523.832-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Burk... [more]
Match NameE-valueIdentityDescription
A0A6J1IBD43.8e-14693.57pheophytinase, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111471... [more]
A0A6J1I8N73.8e-14693.57pheophytinase, chloroplastic isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111471... [more]
A0A0A0KAI81.6e-13696.47AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014... [more]
A0A5A7U2I76.8e-13594.12Pheophytinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold155G001000... [more]
A0A1S3CIV26.8e-13594.12pheophytinase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501331 ... [more]
Match NameE-valueIdentityDescription
AT5G38520.11.8e-11173.23alpha/beta-Hydrolases superfamily protein [more]
AT5G38520.28.2e-10969.92alpha/beta-Hydrolases superfamily protein [more]
AT5G19850.13.1e-3130.35alpha/beta-Hydrolases superfamily protein [more]
AT4G36530.15.8e-3034.36alpha/beta-Hydrolases superfamily protein [more]
AT4G36530.25.8e-3034.36alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 34..49
score: 41.81
coord: 215..229
score: 45.98
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 26..267
e-value: 1.7E-17
score: 64.7
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 20..276
e-value: 8.0E-45
score: 155.6
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 23..276
NoneNo IPR availablePANTHERPTHR46438:SF7ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 23..279
NoneNo IPR availablePANTHERPTHR46438ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 23..279

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0039303.1Lag0039303.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015994 chlorophyll metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0047746 chlorophyllase activity