Lag0039263 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0039263
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	RNase H domain-containing protein
Location	chr2: 40198605 .. 40199893 (+)
RNA-Seq Expression	Lag0039263
Synteny	Lag0039263

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTGTAAGTTTCACATCAACACAAGGGTGGATCGATATTTTTATTATACCAAAAAATATCGATGAAATGTAAAAATAAGTTACTAAATGTCAAATAAAGTTTCTTAATCTTATCTTATTTTATCTTATCTTGCTATCAAAAAATTGGCTTATTTGGAGAATCATTTCTGGATCATTATGTCCTAATACTAATTTTATAATTCCAAATATATCCCTTAGTTAATTATTACTTCTTTATTGTTAGCAATTTTGTAAATGAATGGGTAAGATTTTTTCTCTATTTTCACCGGAGGAGGCTCGAACTATACTTTCAATCCCTTCAGGCTCACTCTGAGGCTGACCAAAGGATTTGGCATTTCGATTCTTATGTGATTTTTACCGTTAAAAGTGGGTATCGTGTGGCTCAGTCGAGGAATCTTCCTGCGCTTCCTTCCTCGTCGACGACAAATACTTCTTTTTGGTGGAAAGGTATGGAGTATGTCAATTCTAAGCAAAAAAAAAAAATAATAATAATAATAAAAATAAAAAATTGTGGGGTTTATGTTGGGATAGACTTCCTTCAAGAATATCCCTTAATCAGTGGGGTTTGGATGCTTCTCATTTTCTGTGAGAGATTAGGGGAGGATATATGGCATATATTTTGGCTCTATAAAGATGCCCGTAAAGCTTGGTTACTCTCAAAATTTTCTCACTTTTGGCAACCGAATGTAGCATAATTAAGCATACACATCCACTTTATTTTCCTTTAATTTCTGATGGGGCTTCAAGTGGACACTTTGGAACAACCGTAACAAATTTAATTAAGTTCCATGGAATTCTGTCGCATGTCATAAATGGGCCCTCCAGGTCAATAATGAATTGTATGCATGGGTTCCTCTGGTGGAAGGTTGGTACAAGGTAAGTGTTGATGCTAGTTTTGAGTCGAGTTCCATCGTCGTTGCTACTGCTGGGATTATTATTCGTGACCATAGGGGAAGGGTTCTCCTCTCAGCTGCCAGCTGACCTCTAGATTTGTTACTAAATGCCGATTTGGCATAAGGTTTTGCCCTGATGGAGGGTTTTTGCTAGGCACGAGAGATGGGTTTTCGACCAATTCAGATAGAGACTGATTCAATGTGTGTTTTTCGTCTACTTACAGTTTGCCATGAAGACATCTCGAAACTGGGTACTTTAATCAGAAGATTGAAACAAGATGTCGATCAGGCTAATTTGTCGTTCTGGTTTACTCGTCGCTCGGCTAATCAAGCTACTCATACTCTGGCAAAGCTTTGCATGGACTATTCCTGA

mRNA sequence

ATGTTTGCTCACTCTGAGGCTGACCAAAGGATTTGGCATTTCGATTCTTATGTGATTTTTACCGTTAAAAGTGGGTATCGTGTGGCTCAGTCGAGGAATCTTCCTGCGCTTCCTTCCTCGTCGACGACAAATACTTCTTTTTGGTGGAAAGGTTGGTACAAGGTAAGTGTTGATGCTAGTTTTGAGTCGAGTTCCATCGTCGTTGCTACTGCTGGGATTATTATTCGTGACCATAGGGGAAGGGCACGAGAGATGGGTTTTCGACCAATTCAGATAGAGACTGATTCAATGTGTGTTTTTCGTCTACTTACAGTTTGCCATGAAGACATCTCGAAACTGGGTACTTTAATCAGAAGATTGAAACAAGATGTCGATCAGGCTAATTTGTCGTTCTGGTTTACTCGTCGCTCGGCTAATCAAGCTACTCATACTCTGGCAAAGCTTTGCATGGACTATTCCTGA

Coding sequence (CDS)

ATGTTTGCTCACTCTGAGGCTGACCAAAGGATTTGGCATTTCGATTCTTATGTGATTTTTACCGTTAAAAGTGGGTATCGTGTGGCTCAGTCGAGGAATCTTCCTGCGCTTCCTTCCTCGTCGACGACAAATACTTCTTTTTGGTGGAAAGGTTGGTACAAGGTAAGTGTTGATGCTAGTTTTGAGTCGAGTTCCATCGTCGTTGCTACTGCTGGGATTATTATTCGTGACCATAGGGGAAGGGCACGAGAGATGGGTTTTCGACCAATTCAGATAGAGACTGATTCAATGTGTGTTTTTCGTCTACTTACAGTTTGCCATGAAGACATCTCGAAACTGGGTACTTTAATCAGAAGATTGAAACAAGATGTCGATCAGGCTAATTTGTCGTTCTGGTTTACTCGTCGCTCGGCTAATCAAGCTACTCATACTCTGGCAAAGCTTTGCATGGACTATTCCTGA

Protein sequence

MFAHSEADQRIWHFDSYVIFTVKSGYRVAQSRNLPALPSSSTTNTSFWWKGWYKVSVDASFESSSIVVATAGIIIRDHRGRAREMGFRPIQIETDSMCVFRLLTVCHEDISKLGTLIRRLKQDVDQANLSFWFTRRSANQATHTLAKLCMDYS

Homology

[Close]

BLAST of Lag0039263 vs. NCBI nr
Match: XP_022139684.1 (uncharacterized protein LOC111010533 [Momordica charantia])

HSP 1 Score: 58.5 bits (140), Expect = 5.7e-05
Identity = 39/128 (30.47%), Postives = 58/128 (45.31%), Query Frame = 0


Query: 50  KGWYKVSVDASFESSSIVVATAGIIIRDHRGR---------------------------- 109
           +G +K+  DASF S         IIIRDHRG+                            
Sbjct: 95  EGVFKLKTDASFSSIDFNAGLGVIIIRDHRGQVLASATKYLEHVASVDDAEALAAVEGLR 154

Query: 110 -AREMGFRPIQIETDSMCVFRLLTVCHEDISKLGTLIRRLKQDV-DQANLSFWFTRRSAN 148
            A E G  PI +ETDS+ ++ L     E +SK G++I  +K  +  +  +S+ FT+R+ N
Sbjct: 155 VAMETGISPILLETDSLRIYNLFARDKEGLSKTGSIIEYVKTHLATRLQVSYSFTKRTGN 214

[Close]

BLAST of Lag0039263 vs. NCBI nr
Match: XP_022150944.1 (uncharacterized protein LOC111018973 [Momordica charantia])

HSP 1 Score: 55.1 bits (131), Expect = 6.3e-04
Identity = 50/159 (31.45%), Postives = 66/159 (41.51%), Query Frame = 0


Query: 26  YRVAQSRNLPALPSSSTTNTSFW---WKGWYKVSVDASFESSSIVVATAGIIIRDHRG-- 85
           Y+ AQ  +  AL        + W        KV+VDA+F   S  VA  G+IIRD  G  
Sbjct: 82  YQAAQRSSXSALVVCRPPRVAIWRPPAAPLLKVNVDAAFRKESF-VAGVGVIIRDSTGLV 141

Query: 86  ---------------------------RAREMGFRPIQIETDSMCVFRLLTVCHEDISKL 145
                                       A E GF   QIETDS+ +F LLT    D S++
Sbjct: 142 YLTAIRLLARASDVDWVEGFAVYEGILLAVEAGFIRFQIETDSLRIFNLLTTDCVDDSEV 201

Query: 146 GTLIRRLKQDVDQ--ANLSFWFTRRSANQATHTLAKLCM 151
           G L   +K  +      +SF FT R+ N   H LA+L +
Sbjct: 202 GVLCSVIKLFLSSHAERVSFSFTHRNGNAXAHLLAQLAL 239

[Close]

BLAST of Lag0039263 vs. NCBI nr
Match: MBA0632353.1 (hypothetical protein [Gossypium davidsonii] >MBA0668118.1 hypothetical protein [Gossypium klotzschianum])

HSP 1 Score: 54.7 bits (130), Expect = 8.2e-04
Identity = 36/98 (36.73%), Postives = 52/98 (53.06%), Query Frame = 0


Query: 54  KVSVDASFESSSIVVATAGIIIRDHRGR----AREMGFRPIQIETDSMCVFRLLTVCHED 113
           KV+ DAS+   S   +++GII R++ G     A EMGFR + +E D + V R L    ED
Sbjct: 20  KVNFDASYNQQS-CKSSSGIITRNNEGMVVTVAEEMGFRDVCVEGDVLTVIRKLNFAEED 79

Query: 114 ISKLGTLIRRLKQDVDQ-ANLSFWFTRRSANQATHTLA 147
            S + +LI+ +K    +  +L   F  R  N  TH LA
Sbjct: 80  RSSISSLIKEIKGRAPKFRSLQLRFISREVNAKTHGLA 116

The following BLAST results are available for this feature:

BLAST of Lag0039263 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139684.1	5.7e-05	30.47	uncharacterized protein LOC111010533 [Momordica charantia]	[more]
XP_022150944.1	6.3e-04	31.45	uncharacterized protein LOC111018973 [Momordica charantia]	[more]
MBA0632353.1	8.2e-04	36.73	hypothetical protein [Gossypium davidsonii] >MBA0668118.1 hypothetical protein [...	[more]

BLAST of Lag0039263 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 82..149 e-value: 4.1E-6 score: 26.6
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 74..153 e-value: 9.8E-6 score: 27.5
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 57..147 e-value: 3.43918E-10 score: 52.3164
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 51..151

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0039263.1	Lag0039263.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity