Lag0038343 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0038343
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCCHC-type domain-containing protein
Locationchr2: 15755665 .. 15756552 (-)
RNA-Seq ExpressionLag0038343
SyntenyLag0038343
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGATGACGAGCTAAGGAAGATCCAGGATCTGGAAAATGTACTGGTGTGCAAAATAATAACAGAGAAGTATATTAACCCAGAAATCTTTAAATCTATGATTCCGAAAATCTGGAACATGGAGGGAAAGGTCACTGTCAAGAAAACGGGTGAAAATATGTTCGAATGCTCTTTTAACTCACTAAAAGAAAAAAGGAAAGTGATGGAAGGAAGTCCGTGGATCTACGACATAGCTTTCATTACCTTTGAAGAGATTAAAGGGAATGAAAGATACTCACGACTTTAGTTTAGGTACGCCTCGTTTTGGATTCATTTTACTGACTTGCCAAGAATCTGCTTCACCAGGAAATGGGCAGAGGATCTGGGCAATGCAGTGGGAACCTTTGAACGAGTTGACTTGGACAAGGAGGAGTACGAGGTTGGAAATTCCTTGAGGGTGAGAGTCAGGATTGATATCAAGAGGCCCTTGAGGAGAGGGACGATGATCAGAATCAGAACAAATGCGGAAGAAGAATGGGTAGATATGAGGTTTGAAAAACTCCCAGAATTTTGCTTTGGATGCGGGAGAACTGGCCACCTGGCAAGAGAATGTCCAGAAACTGAAGCCATTAACAGAGACAAACTGGACTATGGAGCATGGCTGAAAAGAGATTTCAATCAAAGAACGAAAATGGGAACCAAGACAGGCGCAGTTCGAAAAGGCTATGAAGCAGGATTGAGAAAAGGGAAATCAAGACCAGAGCAAGAAAATAAAGATGACGATCCGCCAGAGGAGACGAACGGAACGACACCGGAAGATTTGAAAACCAGAAACCAGGGAGTAGTGAAGAAAACGACAAAAAAAAGGCTAGTGAGCAGGCATCGGGAAGTACAGAAGTTATCATGA

mRNA sequence

ATGGAAGATGACGAGCTAAGGAAGATCCAGGATCTGGAAAATGTACTGGTGTGCAAAATAATAACAGAGAAGAAATGGGCAGAGGATCTGGGCAATGCAGTGGGAACCTTTGAACGAGTTGACTTGGACAAGGAGGAGTACGAGGTTGGAAATTCCTTGAGGGTGAGAGTCAGGATTGATATCAAGAGGCCCTTGAGGAGAGGGACGATGATCAGAATCAGAACAAATGCGGAAGAAGAATGGGTAGATATGAGGTTTGAAAAACTCCCAGAATTTTGCTTTGGATGCGGGAGAACTGGCCACCTGGCAAGAGAATGTCCAGAAACTGAAGCCATTAACAGAGACAAACTGGACTATGGAGCATGGCTGAAAAGAGATTTCAATCAAAGAACGAAAATGGGAACCAAGACAGGCGCAGTTCGAAAAGGCTATGAAGCAGGATTGAGAAAAGGGAAATCAAGACCAGAGCAAGAAAATAAAGATGACGATCCGCCAGAGGAGACGAACGGAACGACACCGGAAGATTTGAAAACCAGAAACCAGGGAGTAGTGAAGAAAACGACAAAAAAAAGGCTAGTGAGCAGGCATCGGGAAGTACAGAAGTTATCATGA

Coding sequence (CDS)

ATGGAAGATGACGAGCTAAGGAAGATCCAGGATCTGGAAAATGTACTGGTGTGCAAAATAATAACAGAGAAGAAATGGGCAGAGGATCTGGGCAATGCAGTGGGAACCTTTGAACGAGTTGACTTGGACAAGGAGGAGTACGAGGTTGGAAATTCCTTGAGGGTGAGAGTCAGGATTGATATCAAGAGGCCCTTGAGGAGAGGGACGATGATCAGAATCAGAACAAATGCGGAAGAAGAATGGGTAGATATGAGGTTTGAAAAACTCCCAGAATTTTGCTTTGGATGCGGGAGAACTGGCCACCTGGCAAGAGAATGTCCAGAAACTGAAGCCATTAACAGAGACAAACTGGACTATGGAGCATGGCTGAAAAGAGATTTCAATCAAAGAACGAAAATGGGAACCAAGACAGGCGCAGTTCGAAAAGGCTATGAAGCAGGATTGAGAAAAGGGAAATCAAGACCAGAGCAAGAAAATAAAGATGACGATCCGCCAGAGGAGACGAACGGAACGACACCGGAAGATTTGAAAACCAGAAACCAGGGAGTAGTGAAGAAAACGACAAAAAAAAGGCTAGTGAGCAGGCATCGGGAAGTACAGAAGTTATCATGA

Protein sequence

MEDDELRKIQDLENVLVCKIITEKKWAEDLGNAVGTFERVDLDKEEYEVGNSLRVRVRIDIKRPLRRGTMIRIRTNAEEEWVDMRFEKLPEFCFGCGRTGHLARECPETEAINRDKLDYGAWLKRDFNQRTKMGTKTGAVRKGYEAGLRKGKSRPEQENKDDDPPEETNGTTPEDLKTRNQGVVKKTTKKRLVSRHREVQKLS
Homology
BLAST of Lag0038343 vs. NCBI nr
Match: XP_028122006.1 (uncharacterized protein LOC114319195 [Camellia sinensis])

HSP 1 Score: 99.4 bits (246), Expect = 3.9e-17
Identity = 57/154 (37.01%), Postives = 90/154 (58.44%), Query Frame = 0

Query: 20  IITEKKWAEDLGNAVGTFERVDLDKEEYEV--GNSLRVRVRIDIKRPLRRGTMIRIRTNA 79
           ++  KK  E +GNAVG F  +D+D E+  +  G ++R+RV +D+++PLRRG  + + ++A
Sbjct: 136 VLMNKKVGEIVGNAVGQF--IDMDYEDGGIVWGRTMRIRVALDVRKPLRRGMKLAL-SSA 195

Query: 80  EEEWVDMRFEKLPEFCFGCGRTGHLARECPE----TEAINRDKLDYGAWLKRDFNQRTKM 139
           E  WVD ++E+LP +C+ CGR GH  REC +     +    D L YGAWL+ D N ++K 
Sbjct: 196 EPIWVDFKYERLPIYCYFCGRLGHSERECDDKLSSADGSRVDSLQYGAWLRMD-NIKSKG 255

Query: 140 GTKTGAVRKGYEAGLRKGKSRPEQENKDDDPPEE 168
             + G++ KG       G+  P + N      EE
Sbjct: 256 SRRAGSIDKGVGGTHPGGQGIPLRTNHAQVNQEE 285

BLAST of Lag0038343 vs. NCBI nr
Match: XP_028124075.1 (uncharacterized protein LOC114321128 [Camellia sinensis])

HSP 1 Score: 96.7 bits (239), Expect = 2.5e-16
Identity = 55/154 (35.71%), Postives = 90/154 (58.44%), Query Frame = 0

Query: 20  IITEKKWAEDLGNAVGTFERVDLDKEEYEV--GNSLRVRVRIDIKRPLRRGTMIRIRTNA 79
           ++  K+  + +GNAVG F  +D+D E+  +  G ++R+RV ID+++PLRRG  + + ++A
Sbjct: 136 VLMNKQVGQIVGNAVGQF--IDMDYEDGGIAWGRTMRIRVAIDVRKPLRRGMKLAL-SSA 195

Query: 80  EEEWVDMRFEKLPEFCFGCGRTGHLARECPE----TEAINRDKLDYGAWLKRDFNQRTKM 139
           +  WVD ++E+LP +C+ CGR GH  REC +     +    D L YGAWL+ D N ++K 
Sbjct: 196 DPIWVDFKYERLPIYCYFCGRLGHSDRECDDKLSFADGTRVDSLQYGAWLRMD-NIKSKG 255

Query: 140 GTKTGAVRKGYEAGLRKGKSRPEQENKDDDPPEE 168
             + G++ KG       G+  P + N      EE
Sbjct: 256 SRRAGSIDKGVGGTHPGGQGIPMRTNHAQMNQEE 285

BLAST of Lag0038343 vs. NCBI nr
Match: GAU21788.1 (hypothetical protein TSUD_329120, partial [Trifolium subterraneum])

HSP 1 Score: 93.6 bits (231), Expect = 2.1e-15
Identity = 46/108 (42.59%), Postives = 68/108 (62.96%), Query Frame = 0

Query: 27  AEDLGNAVGTFERVDLDKEEYEVGNSLRVRVRIDIKRPLRRGTMIRIRTNAEEEWVDMRF 86
           A+ LGN VGTFE +D+ KE   +G  LR+RV +D+K+PL+RG+  ++    +E WVD ++
Sbjct: 138 AKKLGNTVGTFEEMDM-KESNRMGKFLRMRVYVDLKKPLKRGS--KLNFQGKEIWVDYKY 197

Query: 87  EKLPEFCFGCGRTGHLARECPETEAINRDKLDY----------GAWLK 125
           E+LP FCF CGR GH  R+C + E  + D++ Y          G WL+
Sbjct: 198 ERLPNFCFACGRIGHQMRDCEDIE--DHDEVQYSELEEKQQAFGPWLR 240

BLAST of Lag0038343 vs. NCBI nr
Match: MCH80348.1 (hypothetical protein [Trifolium medium])

HSP 1 Score: 93.6 bits (231), Expect = 2.1e-15
Identity = 47/108 (43.52%), Postives = 67/108 (62.04%), Query Frame = 0

Query: 27  AEDLGNAVGTFERVDLDKEEYEVGNSLRVRVRIDIKRPLRRGTMIRIRTNAEEEWVDMRF 86
           A+ LGN VGTFE +D  KE   +G  LR+RV +D+K+PL+RGT  ++    +E WVD ++
Sbjct: 138 AKKLGNTVGTFEEMDF-KEINRMGKFLRMRVSVDLKKPLKRGT--KLNFQGKEIWVDYKY 197

Query: 87  EKLPEFCFGCGRTGHLARECPETEAINRDKLDY----------GAWLK 125
           E+LP FCF CGR GH  R+C + E  + D++ Y          G WL+
Sbjct: 198 ERLPNFCFACGRIGHQMRDCEDIE--DHDEVQYSELEEKQQAFGPWLR 240

BLAST of Lag0038343 vs. NCBI nr
Match: XP_028071384.1 (uncharacterized protein LOC114273772 [Camellia sinensis])

HSP 1 Score: 92.8 bits (229), Expect = 3.6e-15
Identity = 55/154 (35.71%), Postives = 87/154 (56.49%), Query Frame = 0

Query: 20  IITEKKWAEDLGNAVGTFERVDLDKEEYEV--GNSLRVRVRIDIKRPLRRGTMIRIRTNA 79
           I+  KK  + +GNAVG F  +D+D E+  +  G ++ +RV +D+++PLRRG  + + ++ 
Sbjct: 136 ILMNKKVGQIVGNAVGQF--IDMDYEDGGIAWGRTMLIRVALDVRKPLRRGMKLAL-SSV 195

Query: 80  EEEWVDMRFEKLPEFCFGCGRTGHLAREC----PETEAINRDKLDYGAWLKRDFNQRTKM 139
           E  WVD ++E+LP +C+ CGR GH  REC       +    D L YGAWL+ D N ++K 
Sbjct: 196 EPIWVDFKYERLPIYCYFCGRLGHSDRECDAKLSSADGTRVDSLQYGAWLRMD-NIKSKG 255

Query: 140 GTKTGAVRKGYEAGLRKGKSRPEQENKDDDPPEE 168
             + G++ KG       G+  P + N      EE
Sbjct: 256 PRRAGSIDKGVGGTHPGGQGIPLRTNHAQVNQEE 285

BLAST of Lag0038343 vs. ExPASy TrEMBL
Match: A0A2Z6LV25 (Uncharacterized protein (Fragment) OS=Trifolium subterraneum OX=3900 GN=TSUD_329120 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 1.0e-15
Identity = 46/108 (42.59%), Postives = 68/108 (62.96%), Query Frame = 0

Query: 27  AEDLGNAVGTFERVDLDKEEYEVGNSLRVRVRIDIKRPLRRGTMIRIRTNAEEEWVDMRF 86
           A+ LGN VGTFE +D+ KE   +G  LR+RV +D+K+PL+RG+  ++    +E WVD ++
Sbjct: 138 AKKLGNTVGTFEEMDM-KESNRMGKFLRMRVYVDLKKPLKRGS--KLNFQGKEIWVDYKY 197

Query: 87  EKLPEFCFGCGRTGHLARECPETEAINRDKLDY----------GAWLK 125
           E+LP FCF CGR GH  R+C + E  + D++ Y          G WL+
Sbjct: 198 ERLPNFCFACGRIGHQMRDCEDIE--DHDEVQYSELEEKQQAFGPWLR 240

BLAST of Lag0038343 vs. ExPASy TrEMBL
Match: A0A392M033 (CCHC-type domain-containing protein (Fragment) OS=Trifolium medium OX=97028 GN=A2U01_0001115 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 1.0e-15
Identity = 47/108 (43.52%), Postives = 67/108 (62.04%), Query Frame = 0

Query: 27  AEDLGNAVGTFERVDLDKEEYEVGNSLRVRVRIDIKRPLRRGTMIRIRTNAEEEWVDMRF 86
           A+ LGN VGTFE +D  KE   +G  LR+RV +D+K+PL+RGT  ++    +E WVD ++
Sbjct: 138 AKKLGNTVGTFEEMDF-KEINRMGKFLRMRVSVDLKKPLKRGT--KLNFQGKEIWVDYKY 197

Query: 87  EKLPEFCFGCGRTGHLARECPETEAINRDKLDY----------GAWLK 125
           E+LP FCF CGR GH  R+C + E  + D++ Y          G WL+
Sbjct: 198 ERLPNFCFACGRIGHQMRDCEDIE--DHDEVQYSELEEKQQAFGPWLR 240

BLAST of Lag0038343 vs. ExPASy TrEMBL
Match: A0A803PUL2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.3e-15
Identity = 48/110 (43.64%), Postives = 62/110 (56.36%), Query Frame = 0

Query: 24  KKWAEDLGNAVGTFERVDLDKEEYEVGNSLRVRVRIDIKRPLRRGTMIRIRTNAEEEWVD 83
           K  AE LGN +G F  V  D      G+ LRVRV +   +PL RG MIR+    +E WVD
Sbjct: 142 KLLAEALGNIIGEFLEVFDDSTNEGWGHFLRVRVNLCTNKPLLRGQMIRLPRIKDEFWVD 201

Query: 84  MRFEKLPEFCFGCGRTGHLAREC----PETEAINRDKLDYGAWLKRDFNQ 130
            R+E+LPEFCF CGR GH    C       ++ N D   YG W+K +F++
Sbjct: 202 FRYERLPEFCFECGRLGHPFERCVAFIERMDSGNDDDFQYGPWMKGEFSK 251

BLAST of Lag0038343 vs. ExPASy TrEMBL
Match: A0A803QC75 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.5e-14
Identity = 45/105 (42.86%), Postives = 60/105 (57.14%), Query Frame = 0

Query: 24  KKWAEDLGNAVGTFERVDLDKEEYEVGNSLRVRVRIDIKRPLRRGTMIRIRTNAEEEWVD 83
           K  AE LGN +G F  V  D      G  LRVRV++ + +PL RG MI++    +E W+D
Sbjct: 140 KALAEALGNIIGEFIEVFEDSTNEGWGPFLRVRVKLSVAKPLPRGQMIKLPKVKDEFWID 199

Query: 84  MRFEKLPEFCFGCGRTGHLAREC----PETEAINRDKLDYGAWLK 125
            R+E++PEFCF CG  GH   +C       ++ N D L YG WLK
Sbjct: 200 YRYERIPEFCFECGCLGHPFEQCIAFMERIDSGNDDDLPYGPWLK 244

BLAST of Lag0038343 vs. ExPASy TrEMBL
Match: A0A392MB81 (Zinc CCHC-type-like protein (Fragment) OS=Trifolium medium OX=97028 GN=A2U01_0005191 PE=4 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.9e-14
Identity = 39/89 (43.82%), Postives = 62/89 (69.66%), Query Frame = 0

Query: 27  AEDLGNAVGTFERVDLDKEEYEVGNSLRVRVRIDIKRPLRRGTMIRIRTNAEEEWVDMRF 86
           A+ LGN +GTFE +D+ KE   +G  LR+R  +D+++PL+RG+  +++   ++ WVD ++
Sbjct: 97  AKKLGNNLGTFEEMDM-KECNRMGKFLRIRALLDLRKPLKRGS--KLKFQGKDIWVDYKY 156

Query: 87  EKLPEFCFGCGRTGHLARECPETEAINRD 116
           E+LP FCFGCGR GH  ++C E E  + D
Sbjct: 157 ERLPNFCFGCGRIGHQMKDCEEVEDHDED 182

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_028122006.13.9e-1737.01uncharacterized protein LOC114319195 [Camellia sinensis][more]
XP_028124075.12.5e-1635.71uncharacterized protein LOC114321128 [Camellia sinensis][more]
GAU21788.12.1e-1542.59hypothetical protein TSUD_329120, partial [Trifolium subterraneum][more]
MCH80348.12.1e-1543.52hypothetical protein [Trifolium medium][more]
XP_028071384.13.6e-1535.71uncharacterized protein LOC114273772 [Camellia sinensis][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A2Z6LV251.0e-1542.59Uncharacterized protein (Fragment) OS=Trifolium subterraneum OX=3900 GN=TSUD_329... [more]
A0A392M0331.0e-1543.52CCHC-type domain-containing protein (Fragment) OS=Trifolium medium OX=97028 GN=A... [more]
A0A803PUL21.3e-1543.64Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QC751.5e-1442.86Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A392MB811.9e-1443.82Zinc CCHC-type-like protein (Fragment) OS=Trifolium medium OX=97028 GN=A2U01_000... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001878Zinc finger, CCHC-typeSMARTSM00343c2hcfinal6coord: 92..108
e-value: 6.3E-4
score: 29.0
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 93..108
score: 11.004309
NoneNo IPR availableGENE3D4.10.60.10coord: 90..115
e-value: 3.0E-6
score: 28.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..173
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 133..203
NoneNo IPR availablePANTHERPTHR31286:SF84SUBFAMILY NOT NAMEDcoord: 23..165
IPR025836Zinc knuckle CX2CX4HX4CPFAMPF14392zf-CCHC_4coord: 59..107
e-value: 7.5E-13
score: 48.0
IPR040256Uncharacterized protein At4g02000-likePANTHERPTHR31286GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1.8-LIKEcoord: 23..165
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 91..111

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0038343.1Lag0038343.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding