Lag0038271 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0038271
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cytochrome P450
Location	chr2: 14620001 .. 14620480 (-)
RNA-Seq Expression	Lag0038271
Synteny	Lag0038271

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGACCTTGAGGCGGCATCCGTCGGGGCATTTTGCTCTGCCGCATTTAACAACTGAGAAAGTAGTGTTGGACGGGAAATTCGTGGTGCCGGAGAGAGTGACGGTGCATTTTGCGGCGGCGGAGATGGCGAGGGACGGAGAGGTGTGGAAGGAGCCAATGGAGTTCCAGCTGGAGAGATTCTTGATGAACGAAGAAGACGATGCAGTGGAGGTTTTGACGGGGAACAAGGAGATTAGGTTTATGCCATTTGGGGGAGGGAGAAGAATCTGCCCTTGGATTGGGATTTCAATGCTTCATTTGCAGTACTTTGTGGGGAATTTGGTGTGGATTTCAATGCTTCACATCCTCCCAAATTTCCTTTGCTTAGAAGCTTATCCCAGAAGCACTCCCCCCCACTCCTTCGTCGGTGGTACACTCCTAAACGGCGGCACAATGATGCAGTTGGACGGCAAGGGCAAATTAGCGCAGAGGGAATAA

mRNA sequence

ATGGAGACCTTGAGGCGGCATCCGTCGGGGCATTTTGCTCTGCCGCATTTAACAACTGAGAAAGTAGTGTTGGACGGGAAATTCGTGGTGCCGGAGAGAGTGACGGTGCATTTTGCGGCGGCGGAGATGGCGAGGGACGGAGAGGTGTGGAAGGAGCCAATGGAGTTCCAGCTGGAGAGATTCTTGATGAACGAAGAAGACGATGCAGTGGAGGTTTTGACGGGGAACAAGGAGATTAGGTTTATGCCATTTGGGGGAGGGAGAAGAATCTGCCCTTGGATTGGGATTTCAATGCTTCATTTGCAGTACTTTGTGGGGAATTTGGTGTGGATTTCAATGCTTCACATCCTCCCAAATTTCCTTTGCTTAGAAGCTTATCCCAGAAGCACTCCCCCCCACTCCTTCGTCGGTGGTACACTCCTAAACGGCGGCACAATGATGCAGTTGGACGGCAAGGGCAAATTAGCGCAGAGGGAATAA

Coding sequence (CDS)

ATGGAGACCTTGAGGCGGCATCCGTCGGGGCATTTTGCTCTGCCGCATTTAACAACTGAGAAAGTAGTGTTGGACGGGAAATTCGTGGTGCCGGAGAGAGTGACGGTGCATTTTGCGGCGGCGGAGATGGCGAGGGACGGAGAGGTGTGGAAGGAGCCAATGGAGTTCCAGCTGGAGAGATTCTTGATGAACGAAGAAGACGATGCAGTGGAGGTTTTGACGGGGAACAAGGAGATTAGGTTTATGCCATTTGGGGGAGGGAGAAGAATCTGCCCTTGGATTGGGATTTCAATGCTTCATTTGCAGTACTTTGTGGGGAATTTGGTGTGGATTTCAATGCTTCACATCCTCCCAAATTTCCTTTGCTTAGAAGCTTATCCCAGAAGCACTCCCCCCCACTCCTTCGTCGGTGGTACACTCCTAAACGGCGGCACAATGATGCAGTTGGACGGCAAGGGCAAATTAGCGCAGAGGGAATAA

Protein sequence

METLRRHPSGHFALPHLTTEKVVLDGKFVVPERVTVHFAAAEMARDGEVWKEPMEFQLERFLMNEEDDAVEVLTGNKEIRFMPFGGGRRICPWIGISMLHLQYFVGNLVWISMLHILPNFLCLEAYPRSTPPHSFVGGTLLNGGTMMQLDGKGKLAQRE

Homology

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BLAST of Lag0038271 vs. NCBI nr
Match: XP_038902414.1 (cytochrome P450 89A2-like [Benincasa hispida])

HSP 1 Score: 194.1 bits (492), Expect = 9.0e-46
Identity = 91/112 (81.25%), Postives = 101/112 (90.18%), Query Frame = 0


Query: 1   METLRRHPSGHFALPHLTTEKVVLDGKFVVPERVTVHFAAAEMARDGEVWKEPMEFQLER 60
           METLRRHP GH ALPHL TE+VVL+GKFVVPE+ TVHFAAAEMAR+GEVW+EP+EF+ ER
Sbjct: 196 METLRRHPPGHLALPHLVTEEVVLEGKFVVPEKTTVHFAAAEMARNGEVWEEPLEFRAER 255

Query: 61  FLMN-EEDDAVEVLTGNKEIRFMPFGGGRRICPWIGISMLHLQYFVGNLVWI 112
           FL   EE+D VEVLTG KEIRFMPFGGGRRICP IG+SMLHLQYFVGNLVW+
Sbjct: 256 FLRKAEEEDEVEVLTGKKEIRFMPFGGGRRICPGIGVSMLHLQYFVGNLVWL 307

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BLAST of Lag0038271 vs. NCBI nr
Match: XP_008462791.1 (PREDICTED: cytochrome P450 89A2-like [Cucumis melo] >KAA0066308.1 cytochrome P450 89A2-like [Cucumis melo var. makuwa] >TYK00969.1 cytochrome P450 89A2-like [Cucumis melo var. makuwa])

HSP 1 Score: 191.4 bits (485), Expect = 5.8e-45
Identity = 90/112 (80.36%), Postives = 100/112 (89.29%), Query Frame = 0


Query: 1   METLRRHPSGHFALPHLTTEKVVLDGKFVVPERVTVHFAAAEMARDGEVWKEPMEFQLER 60
           METLRRHP GH ALPHL TE+VVLDGKFVVPE+ TVHFAAAEMAR+G+VW+EPMEF+ ER
Sbjct: 378 METLRRHPPGHLALPHLVTEEVVLDGKFVVPEKTTVHFAAAEMARNGKVWEEPMEFRAER 437

Query: 61  FL-MNEEDDAVEVLTGNKEIRFMPFGGGRRICPWIGISMLHLQYFVGNLVWI 112
           FL   EE++  E+LTG KEIRFMPFGGGRRICP IGISMLHLQYFVGNLVW+
Sbjct: 438 FLRKEEEEEEEEILTGKKEIRFMPFGGGRRICPGIGISMLHLQYFVGNLVWL 489

[Close]

BLAST of Lag0038271 vs. NCBI nr
Match: KGN63354.1 (hypothetical protein Csa_022297 [Cucumis sativus])

HSP 1 Score: 189.9 bits (481), Expect = 1.7e-44
Identity = 88/112 (78.57%), Postives = 100/112 (89.29%), Query Frame = 0


Query: 1   METLRRHPSGHFALPHLTTEKVVLDGKFVVPERVTVHFAAAEMARDGEVWKEPMEFQLER 60
           METLRRHP GH ALPHL TE+VVLDGKFVVPE+ TVHFAAAEMAR+GEVW+EPMEF+ ER
Sbjct: 378 METLRRHPPGHLALPHLVTEEVVLDGKFVVPEKTTVHFAAAEMARNGEVWEEPMEFRAER 437

Query: 61  FL-MNEEDDAVEVLTGNKEIRFMPFGGGRRICPWIGISMLHLQYFVGNLVWI 112
           FL   EE++  E+L+G KEIR+MPFGGGRRICP IGISMLHLQYFVGNL+W+
Sbjct: 438 FLRKEEEEEEEEILSGKKEIRYMPFGGGRRICPGIGISMLHLQYFVGNLIWL 489

[Close]

BLAST of Lag0038271 vs. NCBI nr
Match: XP_011650058.2 (cytochrome P450 89A2 [Cucumis sativus])

HSP 1 Score: 189.9 bits (481), Expect = 1.7e-44
Identity = 88/112 (78.57%), Postives = 100/112 (89.29%), Query Frame = 0


Query: 1   METLRRHPSGHFALPHLTTEKVVLDGKFVVPERVTVHFAAAEMARDGEVWKEPMEFQLER 60
           METLRRHP GH ALPHL TE+VVLDGKFVVPE+ TVHFAAAEMAR+GEVW+EPMEF+ ER
Sbjct: 194 METLRRHPPGHLALPHLVTEEVVLDGKFVVPEKTTVHFAAAEMARNGEVWEEPMEFRAER 253

Query: 61  FL-MNEEDDAVEVLTGNKEIRFMPFGGGRRICPWIGISMLHLQYFVGNLVWI 112
           FL   EE++  E+L+G KEIR+MPFGGGRRICP IGISMLHLQYFVGNL+W+
Sbjct: 254 FLRKEEEEEEEEILSGKKEIRYMPFGGGRRICPGIGISMLHLQYFVGNLIWL 305

[Close]

BLAST of Lag0038271 vs. NCBI nr
Match: XP_023006831.1 (cytochrome P450 89A2-like [Cucurbita maxima])

HSP 1 Score: 178.3 bits (451), Expect = 5.1e-41
Identity = 85/111 (76.58%), Postives = 94/111 (84.68%), Query Frame = 0


Query: 1   METLRRHPSGHFALPHLTTEKVVLDGKFVVPERVTVHFAAAEMARDGEVWKEPMEFQLER 60
           METLRRHP G+ +LPHL T++VVLDGKFVVP++ TVHF AAEMAR+GEVWKEPMEF+ ER
Sbjct: 378 METLRRHPPGYLSLPHLATKEVVLDGKFVVPKKTTVHFVAAEMARNGEVWKEPMEFRPER 437

Query: 61  FLMNE-EDDAVEVLTGNKEIRFMPFGGGRRICPWIGISMLHLQYFVGNLVW 111
           FL  E E   VE L G KEIRFMPFGGGRRICP +GISMLHLQYFV NLVW
Sbjct: 438 FLTKEAEKVEVEALIGRKEIRFMPFGGGRRICPGVGISMLHLQYFVANLVW 488

[Close]

BLAST of Lag0038271 vs. ExPASy Swiss-Prot
Match: Q42602 (Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2)

HSP 1 Score: 122.1 bits (305), Expect = 5.7e-27
Identity = 60/109 (55.05%), Postives = 80/109 (73.39%), Query Frame = 0


Query: 1   METLRRHPSGHFALPHLTTEKVVLDGKFVVPERVTVHFAAAEMARDGEVWKEPMEFQLER 60
           +E LRRHP GHF LPH  TE  VL G + VP+  T++F  AE+ RD   W+EPM F+ ER
Sbjct: 363 LEGLRRHPPGHFLLPHSVTEDTVLGG-YKVPKNGTINFMVAEIGRDPVEWEEPMAFKPER 422

Query: 61  FLMNEEDDAVEVLTGNKEIRFMPFGGGRRICPWIGISMLHLQYFVGNLV 110
           F+   E++AV+ LTG++ I+ MPFG GRRICP IG++MLHL+Y+V N+V
Sbjct: 423 FM--GEEEAVD-LTGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMV 467

[Close]

BLAST of Lag0038271 vs. ExPASy Swiss-Prot
Match: Q9SRQ1 (Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.4e-25
Identity = 53/110 (48.18%), Postives = 75/110 (68.18%), Query Frame = 0


Query: 1   METLRRHPSGHFALPHLTTEKVVLDGKFVVPERVTVHFAAAEMARDGEVWKEPMEFQLER 60
           +E LRRHP GH+   H  T   VL G F++P + T++F   EM RD ++W++P+ F+ ER
Sbjct: 366 LECLRRHPPGHYLSYHKVTHDTVLGG-FLIPRQGTINFMVGEMGRDPKIWEDPLTFKPER 425

Query: 61  FLMNEEDDAVEVLTGNKEIRFMPFGGGRRICPWIGISMLHLQYFVGNLVW 111
           FL N E    + +TG +EI+ MPFG GRR+CP   +S+LHL+Y+V NLVW
Sbjct: 426 FLENGEACDFD-MTGTREIKMMPFGAGRRMCPGYALSLLHLEYYVANLVW 473

[Close]

BLAST of Lag0038271 vs. ExPASy Swiss-Prot
Match: Q43255 (indolin-2-one monooxygenase OS=Zea mays OX=4577 GN=CYP71C2 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.5e-16
Identity = 47/110 (42.73%), Postives = 64/110 (58.18%), Query Frame = 0


Query: 2   ETLRRHPSGHFALPHLTTEKVVLDGKFV-VPERVTVHFAAAEMARDGEVWKEPMEFQLER 61
           ETLR HP   F LPH +T    +DG FV    RV VH  A  + RD   W++P EF  ER
Sbjct: 394 ETLRLHPPAPFLLPHYSTADSEIDGYFVPAGTRVLVH--AWALGRDRTTWEKPEEFMPER 453

Query: 62  FLMNEEDDAVEVLTGNKEIRFMPFGGGRRICPWIGISMLHLQYFVGNLVW 111
           F+  +E  AV+V    K++RF+PFG GRRICP +      ++  + NL++
Sbjct: 454 FV--QEPGAVDVHMKGKDLRFIPFGSGRRICPGMNFGFATMEVMLANLMY 499

[Close]

BLAST of Lag0038271 vs. ExPASy Swiss-Prot
Match: P37123 (Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.5e-16
Identity = 43/108 (39.81%), Postives = 62/108 (57.41%), Query Frame = 0


Query: 2   ETLRRHPSGHFALPHLTTEKVVLDGKFVVPERVTVHFAAAEMARDGEVWKEPMEFQLERF 61
           E LR+HP  +F L H  TE V L G + +P    V F    ++ D  VW +P +F  +RF
Sbjct: 358 ELLRKHPPTYFTLTHSVTEPVKLAG-YDIPMDTNVEFFVHGISHDPNVWSDPEKFDPDRF 417

Query: 62  LMNEEDDAVEVLTGNKEIRFMPFGGGRRICPWIGISMLHLQYFVGNLV 110
           L   ED  +   TG KE++ MPFG GRRICP +G++ +H+   +  +V
Sbjct: 418 LSGREDADI---TGVKEVKMMPFGVGRRICPGLGMATVHVNLMLARMV 461

[Close]

BLAST of Lag0038271 vs. ExPASy Swiss-Prot
Match: Q6Z5I7 (Oryzalexin E synthase OS=Oryza sativa subsp. japonica OX=39947 GN=CYP76M6 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.2e-13
Identity = 37/108 (34.26%), Postives = 59/108 (54.63%), Query Frame = 0


Query: 2   ETLRRHPSGHFALPHLTTEKVVLDGKFVVPERVTVHFAAAEMARDGEVWKEPMEFQLERF 61
           E +R HP G   +PHL  +  V  G + VP+  TV F A  + RD   W+ P +F  ERF
Sbjct: 363 EAMRLHPVGSILVPHLAVQDGVEIGGYAVPKGTTVIFNAWAIMRDPAAWESPDQFLPERF 422

Query: 62  LMNEEDDAVEVLTGNKEIRFMPFGGGRRICPWIGISMLHLQYFVGNLV 110
           L  EE  +  +    K+  ++PFG GRR+CP + ++   + + + +L+
Sbjct: 423 LHKEESSSPPLELRGKDYEYIPFGSGRRLCPGLPLAERAVPFILASLL 470

The following BLAST results are available for this feature:

BLAST of Lag0038271 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902414.1	9.0e-46	81.25	cytochrome P450 89A2-like [Benincasa hispida]	[more]
XP_008462791.1	5.8e-45	80.36	PREDICTED: cytochrome P450 89A2-like [Cucumis melo] >KAA0066308.1 cytochrome P45...	[more]
KGN63354.1	1.7e-44	78.57	hypothetical protein Csa_022297 [Cucumis sativus]	[more]
XP_011650058.2	1.7e-44	78.57	cytochrome P450 89A2 [Cucumis sativus]	[more]
XP_023006831.1	5.1e-41	76.58	cytochrome P450 89A2-like [Cucurbita maxima]	[more]

BLAST of Lag0038271 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42602	5.7e-27	55.05	Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2	[more]
Q9SRQ1	2.4e-25	48.18	Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1	[more]
Q43255	4.5e-16	42.73	indolin-2-one monooxygenase OS=Zea mays OX=4577 GN=CYP71C2 PE=1 SV=1	[more]
P37123	4.5e-16	39.81	Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=...	[more]
Q6Z5I7	1.2e-13	34.26	Oryzalexin E synthase OS=Oryza sativa subsp. japonica OX=39947 GN=CYP76M6 PE=1 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 39..63 score: 30.73 coord: 81..91 score: 59.02
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..108 e-value: 2.4E-21 score: 76.1
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..119 e-value: 6.7E-24 score: 86.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 2..118
None	No IPR available	PANTHER	PTHR24298:SF850	CYTOCHROME P450	coord: 1..110
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 1..110

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0038271.1	Lag0038271.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen