Lag0038265 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0038265
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	basic blue protein-like
Location	chr2: 14526791 .. 14528860 (+)
RNA-Seq Expression	Lag0038265
Synteny	Lag0038265

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCGATGCAGGAAGAGCGGCAGGGCGGTTTCGATGAGTGTGGTGGCCATTGCGGTGGCGTCGCTGTTGCTGCTTGCTTTGGAACATGGCGATGCCGCTGCGACAACTTTTATGGTTGGAGATTCGAATGGATGGAACATGGATATGAAGGAATGGCCAAATGGGAAGGTGTTTAGAGCTGGTGATATACTAAGTGAGTTTTCAGATCTCTATTTTGAATTATTTTGATTGTATGCTCTTTCTTCTTCTTTTTGTGAAGAAGTAAAAATTTCGTTTAAATATTCTTTTGATTCTTGAAAATTCATGTTTTTTTTTTTTATTTAATGTCTAAACATTTGAATATTTTGTTTAGGTTGAAGGAATAAAAGAAAATTAAAGAAAATTTGTGGCTAGATTAAAATATTTGAAAGCTAAGAATTTAATTTCAAATAAGTTTATTCAAAAAAAAAAAAAAAAAAGAAGAATCAAATCAAATAAGTAAGAAAGTTTATGGAGATGAAATATTCAAGTTTACAATCCTGACATACTTTTATGTCCATTAAGATTTCCCTTTTAGTTATTCAAGGGTAAAAGGAGGAAAAAAAAAGTTAACTTTTAACCTTGAATTTAAATTTAGGTTTAAATCCTATTTTAGTCCCTAAACTCTCAAGTTTATTATGTTTTGGTCCTTAAACTTTTAAATTGTTCATTTGAGTCCTTAAACTTTCAAGTTTGTTCTATTTTGGTTCTTGAACTTTTAAAATGTGTCTATTTTAATCCTTGAGCTTAAAAAAAACAACTATTTTGGTCCTCTCTTTTATTTCAATCTTAACAAATTAAAGACCCATCTTTGAGTATTTGTATTAGCATGCTAATATGAGTAGGATTTCAAGTCAAATAAGTTAGAGATAAACTCGAACAAAGCGAATTTAAGGTAAAAAAGTAACGTAGTAAGGACCAAATTGGTTATTTCTAAAAAGTTTTAGGGACTAAAATAAACATTTTGAAAGTTTAGACACTAAAATAGAACAAATCCAGAAGTTTAAGAACCAAAATAGATATTTTGAAAGTTTAGTGACCAAAATAGAACAAACTTGAAAGTTCTTGGACTAAAATGAGATTTTAACCTTACAAATTATCAATAATGTAGATCTTAAACCTAACTTTCAATTAAAAAAATTGATCTTACAGTTACAAAAATTTACACTTTCTCAGATTGAAATTCAAACACCAAAATCAGAGGCACATAAATGCAAAAAAAAAAAAAAAAAACTCTACTTCCATCCAACATTGCCACACAATCATGCTAATAAAAAAAATAATGTATGTACAACACCATCAAAAAGCACCATTTATGCATAAAAATAGTTTCTCAAGCTAAAAATACAACCTACCTTGAGTGAACATAACTTAGTGATAATTGTCATGTACCTTTCGATTAGGAGGTCAGAGGTTTGAATTCCCCACTCCAAATGTTGTTGAACAAAAAAAAAAAACACAAAGAATCTTTAAGGAAAAGAAAAAAGAAAAAAGAAATACACCAGCAATAGAGTACTAATTTCATAATCATTTTTTGTTGGTATAGTGGTCAATAAAAGCAACCCAAGAAAACATGGTAAGAGAACTTTGGAGAAATTGGGTCAAACCTTATGATGACTACCTTTCTAAACAACGTTAGGATTAGATTGTTTATTCTTTTCAGTTTTCATCTTATTAATTTCTTTTTCGAGTTTAAGAATAAAAAAATGTGGAGGACCGAACCATCAACTTTTAAGATAGTATTAGGTATCTTATCTGTTTGAATAATCAACGGTTAGATTAATGTTAATAACAAGAAAAAAATGATATTTTTAATAACAATAAAAACGAATGTGTGCAATTTTTGCAGAATTCAACTACGACCCAAGAGTCCACAATGTTGTGGCAGTGGATGAGATAGGTTATAGCAGCTGCACAGCTCCAAGAGGAGCCAAAGTGTTTGATTCAGGCATGGATGAGATCAAGCTCTCAAGAGGACACAACTTCTTCATATGCACCTTACCAGGCCACTGTCAAGCTGGGATGAAGATTGCCATCACTACCCTCTGA

mRNA sequence

ATGGCTCGATGCAGGAAGAGCGGCAGGGCGGTTTCGATGAGTGTGGTGGCCATTGCGGTGGCGTCGCTGTTGCTGCTTGCTTTGGAACATGGCGATGCCGCTGCGACAACTTTTATGGTTGGAGATTCGAATGGATGGAACATGGATATGAAGGAATGGCCAAATGGGAAGGTGTTTAGAGCTGGTGATATACTAAAATTCAACTACGACCCAAGAGTCCACAATGTTGTGGCAGTGGATGAGATAGGTTATAGCAGCTGCACAGCTCCAAGAGGAGCCAAAGTGTTTGATTCAGGCATGGATGAGATCAAGCTCTCAAGAGGACACAACTTCTTCATATGCACCTTACCAGGCCACTGTCAAGCTGGGATGAAGATTGCCATCACTACCCTCTGA

Coding sequence (CDS)

ATGGCTCGATGCAGGAAGAGCGGCAGGGCGGTTTCGATGAGTGTGGTGGCCATTGCGGTGGCGTCGCTGTTGCTGCTTGCTTTGGAACATGGCGATGCCGCTGCGACAACTTTTATGGTTGGAGATTCGAATGGATGGAACATGGATATGAAGGAATGGCCAAATGGGAAGGTGTTTAGAGCTGGTGATATACTAAAATTCAACTACGACCCAAGAGTCCACAATGTTGTGGCAGTGGATGAGATAGGTTATAGCAGCTGCACAGCTCCAAGAGGAGCCAAAGTGTTTGATTCAGGCATGGATGAGATCAAGCTCTCAAGAGGACACAACTTCTTCATATGCACCTTACCAGGCCACTGTCAAGCTGGGATGAAGATTGCCATCACTACCCTCTGA

Protein sequence

MARCRKSGRAVSMSVVAIAVASLLLLALEHGDAAATTFMVGDSNGWNMDMKEWPNGKVFRAGDILKFNYDPRVHNVVAVDEIGYSSCTAPRGAKVFDSGMDEIKLSRGHNFFICTLPGHCQAGMKIAITTL

Homology

[Close]

BLAST of Lag0038265 vs. NCBI nr
Match: KAG6571300.1 (hypothetical protein SDJN03_30215, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 204.5 bits (519), Expect = 5.5e-49
Identity = 101/131 (77.10%), Postives = 110/131 (83.97%), Query Frame = 0


Query: 1   MARCRKSGRAVSMSVVAIAVASLLLLALEHGDAAATTFMVGDSNGWNMDMKEWPNGKVFR 60
           MAR   S  AVSMS VA+A+A LLLL  EHGD AATTF+VGDSNGWN+  + WPNGKVFR
Sbjct: 1   MARHEGSSGAVSMSTVAVAMALLLLLGFEHGD-AATTFVVGDSNGWNLGTEGWPNGKVFR 60

Query: 61  AGDILKFNYDPRVHNVVAVDEIGYSSCTAPRGAKVFDSGMDEIKLSRGHNFFICTLPGHC 120
           AGDIL+FNY+P VHNVVAVDE GY SC APRGAKVF+SG DEI LSRGHNFFICT PGHC
Sbjct: 61  AGDILRFNYNPTVHNVVAVDEGGYRSCIAPRGAKVFNSGKDEIILSRGHNFFICTTPGHC 120

Query: 121 QAGMKIAITTL 132
           QAGMKIAI +L
Sbjct: 121 QAGMKIAINSL 130

[Close]

BLAST of Lag0038265 vs. NCBI nr
Match: XP_022981649.1 (basic blue protein-like [Cucurbita maxima])

HSP 1 Score: 202.2 bits (513), Expect = 2.7e-48
Identity = 99/131 (75.57%), Postives = 110/131 (83.97%), Query Frame = 0


Query: 1   MARCRKSGRAVSMSVVAIAVASLLLLALEHGDAAATTFMVGDSNGWNMDMKEWPNGKVFR 60
           MAR   S  AVSMS VA+A+A LLLL  EHGD AATTF+VGDSNGWN+  + WPNGKVFR
Sbjct: 1   MARHEGSSGAVSMSTVAVAMALLLLLGFEHGD-AATTFVVGDSNGWNLGTEGWPNGKVFR 60

Query: 61  AGDILKFNYDPRVHNVVAVDEIGYSSCTAPRGAKVFDSGMDEIKLSRGHNFFICTLPGHC 120
           AGDIL+FNY+P VHNVVAVDE GY +C APRGAKVF+SG DEI LSRG NFFICT+PGHC
Sbjct: 61  AGDILRFNYNPTVHNVVAVDEGGYRNCIAPRGAKVFNSGKDEIMLSRGRNFFICTIPGHC 120

Query: 121 QAGMKIAITTL 132
           QAGMKIAI +L
Sbjct: 121 QAGMKIAINSL 130

[Close]

BLAST of Lag0038265 vs. NCBI nr
Match: XP_022981647.1 (basic blue protein-like [Cucurbita maxima])

HSP 1 Score: 202.2 bits (513), Expect = 2.7e-48
Identity = 100/131 (76.34%), Postives = 109/131 (83.21%), Query Frame = 0


Query: 1   MARCRKSGRAVSMSVVAIAVASLLLLALEHGDAAATTFMVGDSNGWNMDMKEWPNGKVFR 60
           MAR   S  AVSMS VA+A+A LLLL  EHGD AATTF+VGDSNGWN+  + WPNGKVFR
Sbjct: 1   MARHEGSSGAVSMSTVAVAMALLLLLGFEHGD-AATTFVVGDSNGWNLGTEGWPNGKVFR 60

Query: 61  AGDILKFNYDPRVHNVVAVDEIGYSSCTAPRGAKVFDSGMDEIKLSRGHNFFICTLPGHC 120
           AGDIL+FNY+P VHNVVAVDE GY SC APRGAKVF+SG DEI LSRG NFFICT PGHC
Sbjct: 61  AGDILRFNYNPTVHNVVAVDEGGYRSCIAPRGAKVFNSGKDEIMLSRGRNFFICTTPGHC 120

Query: 121 QAGMKIAITTL 132
           QAGMKIAI +L
Sbjct: 121 QAGMKIAINSL 130

[Close]

BLAST of Lag0038265 vs. NCBI nr
Match: XP_022941118.1 (basic blue protein-like [Cucurbita moschata] >XP_022941139.1 basic blue protein-like [Cucurbita moschata])

HSP 1 Score: 202.2 bits (513), Expect = 2.7e-48
Identity = 100/131 (76.34%), Postives = 109/131 (83.21%), Query Frame = 0


Query: 1   MARCRKSGRAVSMSVVAIAVASLLLLALEHGDAAATTFMVGDSNGWNMDMKEWPNGKVFR 60
           MAR   S  AVSMS VA+A+A LLLL  EHGD AATTF+VGDSNGWN+  + WPNGKVFR
Sbjct: 1   MARHEGSSGAVSMSTVAVAMALLLLLGFEHGD-AATTFVVGDSNGWNLGTEGWPNGKVFR 60

Query: 61  AGDILKFNYDPRVHNVVAVDEIGYSSCTAPRGAKVFDSGMDEIKLSRGHNFFICTLPGHC 120
           AGDIL+FNY+P VHNVVAVDE GY SC APRGAKVF+SG DEI LSRG NFFICT PGHC
Sbjct: 61  AGDILRFNYNPTVHNVVAVDEGGYRSCIAPRGAKVFNSGKDEIMLSRGQNFFICTTPGHC 120

Query: 121 QAGMKIAITTL 132
           QAGMKIAI +L
Sbjct: 121 QAGMKIAINSL 130

[Close]

BLAST of Lag0038265 vs. NCBI nr
Match: KAG6607683.1 (hypothetical protein SDJN03_01025, partial [Cucurbita argyrosperma subsp. sororia] >KAG6607709.1 hypothetical protein SDJN03_01051, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 201.8 bits (512), Expect = 3.6e-48
Identity = 99/131 (75.57%), Postives = 109/131 (83.21%), Query Frame = 0


Query: 1   MARCRKSGRAVSMSVVAIAVASLLLLALEHGDAAATTFMVGDSNGWNMDMKEWPNGKVFR 60
           MAR   S  AVSMS VA+A+A LLLL  EHGD AATTF+VGDSNGWN+  + WPNGKVFR
Sbjct: 1   MARHEGSSGAVSMSTVAVAMALLLLLGFEHGD-AATTFVVGDSNGWNLGTEGWPNGKVFR 60

Query: 61  AGDILKFNYDPRVHNVVAVDEIGYSSCTAPRGAKVFDSGMDEIKLSRGHNFFICTLPGHC 120
           AGDIL+FNY+P VHNVVAVDE GY SC APRGAKVF+SG DEI LSRG NFF+CT PGHC
Sbjct: 61  AGDILRFNYNPTVHNVVAVDEGGYRSCIAPRGAKVFNSGKDEIMLSRGQNFFVCTTPGHC 120

Query: 121 QAGMKIAITTL 132
           QAGMKIAI +L
Sbjct: 121 QAGMKIAINSL 130

[Close]

BLAST of Lag0038265 vs. ExPASy Swiss-Prot
Match: Q8LG89 (Basic blue protein OS=Arabidopsis thaliana OX=3702 GN=ARPN PE=2 SV=2)

HSP 1 Score: 134.0 bits (336), Expect = 1.2e-30
Identity = 64/131 (48.85%), Postives = 85/131 (64.89%), Query Frame = 0


Query: 1   MARCRKSGRAVSMSVVAIAVASLLLLALEHGDAAATTFMVGDSNGWNMDMKEWPNGKVFR 60
           MA+ R S    + ++V +   S+LLL  ++  AA  T+ VGDS  W  +   WP GK FR
Sbjct: 1   MAKGRGSASWSARAIVTLMAVSVLLLQADYVQAA--TYTVGDSGIWTFNAVGWPKGKHFR 60

Query: 61  AGDILKFNYDPRVHNVVAVDEIGYSSCTAPRGAKVFDSGMDEIKLSRGHNFFICTLPGHC 120
           AGD+L FNY+PR+HNVV VD   Y++C  P GAK + SG D I LS+G NFFIC  P HC
Sbjct: 61  AGDVLVFNYNPRMHNVVKVDSGSYNNCKTPTGAKPYTSGKDRITLSKGQNFFICNFPNHC 120

Query: 121 QAGMKIAITTL 132
           ++ MKIA+T +
Sbjct: 121 ESDMKIAVTAV 129

[Close]

BLAST of Lag0038265 vs. ExPASy Swiss-Prot
Match: P00303 (Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.6e-30
Identity = 54/94 (57.45%), Postives = 71/94 (75.53%), Query Frame = 0


Query: 38  FMVGDSNGWNMDMKEWPNGKVFRAGDILKFNYDPRVHNVVAVDEIGYSSCTAPRGAKVFD 97
           ++VG S GW  + + WP GK FRAGDIL FNY+P +HNVV V++ G+S+C  P GAKV+ 
Sbjct: 3   YVVGGSGGWTFNTESWPKGKRFRAGDILLFNYNPXMHNVVVVNQGGFSTCNTPAGAKVYT 62

Query: 98  SGMDEIKLSRGHNFFICTLPGHCQAGMKIAITTL 132
           SG D+IKL +G ++FIC  PGHCQ+GMKIA+  L
Sbjct: 63  SGRDQIKLPKGQSYFICNFPGHCQSGMKIAVNAL 96

[Close]

BLAST of Lag0038265 vs. ExPASy Swiss-Prot
Match: P60496 (Chemocyanin OS=Lilium longiflorum OX=4690 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 2.9e-29
Identity = 62/123 (50.41%), Postives = 77/123 (62.60%), Query Frame = 0


Query: 7   SGRAVSMSVVAIAVASLLLLALEHGDAAATTFMVGDSNGWNMDMKEWPNGKVFRAGDILK 66
           SG A    V+ +    L+ L      A +  + VGD  GW      WP GK FRAGD+L 
Sbjct: 5   SGSAERALVLGVV---LVFLVFNCEVAESVVYTVGDGGGWTFGTSGWPAGKTFRAGDVLV 64

Query: 67  FNYDPRVHNVVAVDEIGYSSCTAPRGAKVFDSGMDEIKLSRGHNFFICTLPGHCQAGMKI 126
           F Y+P VHNVV+V   GY SCTA  G++VF SG D I LSRG N+FIC++PGHCQ G+KI
Sbjct: 65  FKYNPAVHNVVSVPAGGYKSCTASPGSRVFKSGDDRITLSRGTNYFICSVPGHCQGGLKI 124

Query: 127 AIT 130
           A+T
Sbjct: 125 AVT 124

[Close]

BLAST of Lag0038265 vs. ExPASy Swiss-Prot
Match: A0A072U307 (Blue copper protein 1b OS=Medicago truncatula OX=3880 GN=BCP1B PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 4.4e-17
Identity = 46/118 (38.98%), Postives = 66/118 (55.93%), Query Frame = 0


Query: 18  IAVASLLLLALEHGDAAATTFMVGDSNGWNMDM--KEWPNGKVFRAGDILKFNYDPRVHN 77
           + + S+ ++ L     AAT ++VGD  GW +D    +W   KVFR GD L FNYDP  HN
Sbjct: 7   VLILSISMVLLSSVAIAATDYIVGDDKGWTVDFDYTQWAQDKVFRVGDNLVFNYDPSRHN 66

Query: 78  VVAVDEIGYSSCTAPRGAKVFDSGMDEIKL-SRGHNFFICTLPGHCQA-GMKIAITTL 132
           V  V+   + SCT P   +   +G D I+L + G  +++C +  HC A  MK+ IT L
Sbjct: 67  VFKVNGTLFQSCTFPPKNEALSTGKDIIQLKTEGRKWYVCGVADHCSARQMKLVITVL 124

[Close]

BLAST of Lag0038265 vs. ExPASy Swiss-Prot
Match: P80728 (Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 4.4e-17
Identity = 42/99 (42.42%), Postives = 55/99 (55.56%), Query Frame = 0


Query: 35  ATTFMVGDSNGWN----MDMKEWPNGKVFRAGDILKFNYDPRVHNVVAVDEIGYSSCTAP 94
           AT   VGDS GW      D  +W +   F  GD L FNY+ + HNV+ VD+  + SC + 
Sbjct: 1   ATVHKVGDSTGWTTLVPYDYAKWASSNKFHVGDSLLFNYNNKFHNVLQVDQEQFKSCNSS 60

Query: 95  RGAKVFDSGMDEIKLSR-GHNFFICTLPGHCQAGMKIAI 129
             A  + SG D I L R G  +F+C +PGHCQ G K+ I
Sbjct: 61  SPAASYTSGADSIPLKRPGTFYFLCGIPGHCQLGQKVEI 99

The following BLAST results are available for this feature:

BLAST of Lag0038265 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571300.1	5.5e-49	77.10	hypothetical protein SDJN03_30215, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022981649.1	2.7e-48	75.57	basic blue protein-like [Cucurbita maxima]	[more]
XP_022981647.1	2.7e-48	76.34	basic blue protein-like [Cucurbita maxima]	[more]
XP_022941118.1	2.7e-48	76.34	basic blue protein-like [Cucurbita moschata] >XP_022941139.1 basic blue protein-...	[more]
KAG6607683.1	3.6e-48	75.57	hypothetical protein SDJN03_01025, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Lag0038265 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LG89	1.2e-30	48.85	Basic blue protein OS=Arabidopsis thaliana OX=3702 GN=ARPN PE=2 SV=2	[more]
P00303	1.6e-30	57.45	Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
P60496	2.9e-29	50.41	Chemocyanin OS=Lilium longiflorum OX=4690 PE=1 SV=1	[more]
A0A072U307	4.4e-17	38.98	Blue copper protein 1b OS=Medicago truncatula OX=3880 GN=BCP1B PE=2 SV=1	[more]
P80728	4.4e-17	42.42	Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 48..124 e-value: 6.2E-24 score: 83.8
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 36..131 score: 32.07111
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 34..131 e-value: 4.0E-29 score: 102.9
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 37..129
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 13..129
None	No IPR available	PANTHER	PTHR33021:SF242	BASIC BLUE-LIKE PROTEIN	coord: 13..129
IPR041844	Plantacyanin	CDD	cd11013	Plantacyanin	coord: 36..129 e-value: 2.08763E-46 score: 143.649

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0038265.1	Lag0038265.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009055	electron transfer activity