Lag0038256 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0038256
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cysteine proteinase inhibitor 1-like
Location	chr2: 14422398 .. 14422757 (+)
RNA-Seq Expression	Lag0038256
Synteny	Lag0038256

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTCGTATGTGGAGGCTGCCCCGGGACAAAACGATTCCCAGTTCCAAGGCCCAAAATATGTTGGACCTGGTTCTTGGGAACCGATTGAGAACATTCAAGATCCATATGTTCAAGAGATGGGAAGGTTTGCAGTGATGGAGCACAACAAGCAAATAATGGAGAAAATGGGGGCAAATCTTGAGTTCATTTGTGTGGTAAAAGGTGACAAACAAGTGGTGGCTGGAGTGAATTATCGCCTTATAATAGAAGCAAGGAACGAAATGAAAATGGTTTGGAGTTATGAGGCGATTGTGAATGATAGGCCATGGGAGAAGAAGTGGACGCTGATATCTTTTGTACCTTTGCTCAGGAACTAA

mRNA sequence

ATGAGTTCGTATGTGGAGGCTGCCCCGGGACAAAACGATTCCCAGTTCCAAGGCCCAAAATATGTTGGACCTGGTTCTTGGGAACCGATTGAGAACATTCAAGATCCATATGTTCAAGAGATGGGAAGGTTTGCAGTGATGGAGCACAACAAGCAAATAATGGAGAAAATGGGGGCAAATCTTGAGTTCATTTGTGTGGTAAAAGGTGACAAACAAGTGGTGGCTGGAGTGAATTATCGCCTTATAATAGAAGCAAGGAACGAAATGAAAATGGTTTGGAGTTATGAGGCGATTGTGAATGATAGGCCATGGGAGAAGAAGTGGACGCTGATATCTTTTGTACCTTTGCTCAGGAACTAA

Coding sequence (CDS)

ATGAGTTCGTATGTGGAGGCTGCCCCGGGACAAAACGATTCCCAGTTCCAAGGCCCAAAATATGTTGGACCTGGTTCTTGGGAACCGATTGAGAACATTCAAGATCCATATGTTCAAGAGATGGGAAGGTTTGCAGTGATGGAGCACAACAAGCAAATAATGGAGAAAATGGGGGCAAATCTTGAGTTCATTTGTGTGGTAAAAGGTGACAAACAAGTGGTGGCTGGAGTGAATTATCGCCTTATAATAGAAGCAAGGAACGAAATGAAAATGGTTTGGAGTTATGAGGCGATTGTGAATGATAGGCCATGGGAGAAGAAGTGGACGCTGATATCTTTTGTACCTTTGCTCAGGAACTAA

Protein sequence

MSSYVEAAPGQNDSQFQGPKYVGPGSWEPIENIQDPYVQEMGRFAVMEHNKQIMEKMGANLEFICVVKGDKQVVAGVNYRLIIEARNEMKMVWSYEAIVNDRPWEKKWTLISFVPLLRN

Homology

[Close]

BLAST of Lag0038256 vs. NCBI nr
Match: XP_038897049.1 (cysteine proteinase inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 185.7 bits (470), Expect = 2.4e-43
Identity = 90/119 (75.63%), Postives = 101/119 (84.87%), Query Frame = 0


Query: 1   MSSYVEAAPGQNDSQFQGPKYVGPGSWEPIENIQDPYVQEMGRFAVMEHNKQIMEKMGAN 60
           MSSYVE APGQN++  QGPK V  G WEPIENI+DPYVQEMGRF+VMEHNKQ     GAN
Sbjct: 1   MSSYVEVAPGQNEALIQGPKDVELGRWEPIENIEDPYVQEMGRFSVMEHNKQ----TGAN 60

Query: 61  LEFICVVKGDKQVVAGVNYRLIIEARNEMKMVWSYEAIVNDRPWEKKWTLISFVPLLRN 120
           L+FI VV G+KQVVAG+NYRL IEARNEM +VW+YEA+VNDRPWEKKW LI FVPLL+N
Sbjct: 61  LKFIRVVNGEKQVVAGINYRLRIEARNEMNLVWTYEAMVNDRPWEKKWKLIYFVPLLKN 115

[Close]

BLAST of Lag0038256 vs. NCBI nr
Match: XP_022975710.1 (cysteine proteinase inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 154.5 bits (389), Expect = 5.9e-34
Identity = 75/119 (63.03%), Postives = 92/119 (77.31%), Query Frame = 0


Query: 1   MSSYVEAAPGQNDSQFQGPKYVGPGSWEPIENIQDPYVQEMGRFAVMEHNKQIMEKMGAN 60
           MSSYVEAAPGQN++  QG K +  G W+PIE I  PYV+E+GRFAVMEHN+Q     G +
Sbjct: 1   MSSYVEAAPGQNEALIQGSKNLEVGQWKPIEKI-TPYVEEIGRFAVMEHNQQ----SGEH 60

Query: 61  LEFICVVKGDKQVVAGVNYRLIIEARNEMKMVWSYEAIVNDRPWEKKWTLISFVPLLRN 120
           L+FICV++G  Q+VAG NYRLIIEARN + + W+YEA+V D+PWEK W LI FVPLL N
Sbjct: 61  LKFICVIRGWSQIVAGTNYRLIIEARNHVGLAWNYEAMVYDKPWEKTWKLIEFVPLLMN 114

[Close]

BLAST of Lag0038256 vs. NCBI nr
Match: XP_022936213.1 (cysteine proteinase inhibitor 1-like [Cucurbita moschata])

HSP 1 Score: 152.9 bits (385), Expect = 1.7e-33
Identity = 74/119 (62.18%), Postives = 93/119 (78.15%), Query Frame = 0


Query: 1   MSSYVEAAPGQNDSQFQGPKYVGPGSWEPIENIQDPYVQEMGRFAVMEHNKQIMEKMGAN 60
           MSSYVEA PGQN++  Q PK +  G WEPIE I  PYV+E+GRFAVMEHN+Q     G +
Sbjct: 1   MSSYVEADPGQNEALIQDPKNLEFGRWEPIEKI-TPYVEEIGRFAVMEHNQQ----SGEH 60

Query: 61  LEFICVVKGDKQVVAGVNYRLIIEARNEMKMVWSYEAIVNDRPWEKKWTLISFVPLLRN 120
           L+FICV++G  Q+VAG NYRLIIEARN++ + W+YEA+V D+PWEK W LI F+PLL+N
Sbjct: 61  LKFICVLRGWSQLVAGTNYRLIIEARNDVGLAWNYEAMVYDKPWEKTWKLIEFIPLLKN 114

[Close]

BLAST of Lag0038256 vs. NCBI nr
Match: XP_023536174.1 (cysteine proteinase inhibitor 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 152.5 bits (384), Expect = 2.3e-33
Identity = 74/119 (62.18%), Postives = 92/119 (77.31%), Query Frame = 0


Query: 1   MSSYVEAAPGQNDSQFQGPKYVGPGSWEPIENIQDPYVQEMGRFAVMEHNKQIMEKMGAN 60
           MSS+VEAAPGQN++ FQ  K +  G W+PIE+I+DPYVQEMGRFAVMEH++    + G N
Sbjct: 1   MSSHVEAAPGQNEAMFQDKKGIEVGQWKPIEDIKDPYVQEMGRFAVMEHDEM---RRGEN 60

Query: 61  LEFICVVKGDKQVVAGVNYRLIIEARNEMKMVWSYEAIVNDRPWEKKWTLISFVPLLRN 120
           L+FICV  G+KQVVAG+NYRL+I+A N + +   Y AIV D+PWEK W LI FVPLL N
Sbjct: 61  LKFICVQSGEKQVVAGMNYRLLIKASNHLGISSMYMAIVYDKPWEKTWKLIEFVPLLMN 116

[Close]

BLAST of Lag0038256 vs. NCBI nr
Match: KAG6592144.1 (Cysteine proteinase inhibitor 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 152.1 bits (383), Expect = 2.9e-33
Identity = 73/119 (61.34%), Postives = 94/119 (78.99%), Query Frame = 0


Query: 1   MSSYVEAAPGQNDSQFQGPKYVGPGSWEPIENIQDPYVQEMGRFAVMEHNKQIMEKMGAN 60
           MSSYVEAAPGQN++  Q PK +  G WEPIE I + YV+E+GRFAVMEHN+Q     G +
Sbjct: 1   MSSYVEAAPGQNEALIQDPKNLEYGRWEPIEKITE-YVEEIGRFAVMEHNQQ----SGEH 60

Query: 61  LEFICVVKGDKQVVAGVNYRLIIEARNEMKMVWSYEAIVNDRPWEKKWTLISFVPLLRN 120
           L+FICV++G  Q+VAG NYRLII+ARN++ + W+YEA+V D+PWEK W LI F+PLL+N
Sbjct: 61  LKFICVLRGWSQLVAGTNYRLIIKARNDVGLAWNYEAMVYDKPWEKTWKLIEFIPLLKN 114

[Close]

BLAST of Lag0038256 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 8.4e-15
Identity = 45/96 (46.88%), Postives = 61/96 (63.54%), Query Frame = 0


Query: 18  GPKYVGPGSWEPIENIQDPYVQEMGRFAVMEHNKQIMEKMGANLEFICVVKGDKQVVAGV 77
           G K V  G W PIEN+    VQ++ +FAV EHNKQ  ++    L++  VV+G  QVVAG 
Sbjct: 23  GRKRVAAGGWRPIENLNSAEVQDVAQFAVSEHNKQANDE----LQYQSVVRGYTQVVAGT 82

Query: 78  NYRLIIEARNEMKMVWSYEAIVNDRPWEKKWTLISF 114
           NYRL+I A+ +  +V +YEA+V D+PW     L SF
Sbjct: 83  NYRLVIAAK-DGAVVGNYEAVVWDKPWMHFRNLTSF 113

[Close]

BLAST of Lag0038256 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 2.4e-14
Identity = 44/96 (45.83%), Postives = 61/96 (63.54%), Query Frame = 0


Query: 18  GPKYVGPGSWEPIENIQDPYVQEMGRFAVMEHNKQIMEKMGANLEFICVVKGDKQVVAGV 77
           G K V  G W PIE++    VQ++ +FAV EHNKQ  ++    L++  VV+G  QVVAG 
Sbjct: 23  GRKLVAAGGWRPIESLNSAEVQDVAQFAVSEHNKQANDE----LQYQSVVRGYTQVVAGT 82

Query: 78  NYRLIIEARNEMKMVWSYEAIVNDRPWEKKWTLISF 114
           NYRL+I A+ +  +V +YEA+V D+PW     L SF
Sbjct: 83  NYRLVIAAK-DGAVVGNYEAVVWDKPWMHFRNLTSF 113

[Close]

BLAST of Lag0038256 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 1.2e-13
Identity = 42/91 (46.15%), Postives = 55/91 (60.44%), Query Frame = 0


Query: 25  GSWEPIENIQDPYVQEMGRFAVMEHNKQIMEKMGANLEFICVVKGDKQVVAGVNYRLIIE 84
           G W PI N+ DP V E+G FAV E+NK    +  + L+F  VV G+ QVV+G NYRL + 
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNK----RSESGLKFETVVSGETQVVSGTNYRLKVA 88

Query: 85  ARNEMKMVWSYEAIVNDRPWEKKWTLISFVP 116
           A +   +  +Y AIV D+PW K   L SF P
Sbjct: 89  ANDGDGVSKNYLAIVWDKPWMKFRNLTSFEP 115

[Close]

BLAST of Lag0038256 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 3.9e-12
Identity = 42/93 (45.16%), Postives = 58/93 (62.37%), Query Frame = 0


Query: 25  GSWEPIENIQDPYVQEMGRFAVMEHNKQIMEKMGANLEFICVVKGDKQVVAGVNYRLIIE 84
           GSWEP++ I DP+VQE+GR+AV E ++ +       L F  V  G+KQVVAGVNYRL +E
Sbjct: 28  GSWEPVD-INDPHVQELGRWAVAEEDRGV---AAGGLTFERVTDGEKQVVAGVNYRLTLE 87

Query: 85  ARNEMKMVWSYEAIVNDR-PWEKKWTLISFVPL 117
           A +       YEA+V ++ P      L+SF P+
Sbjct: 88  ASSSGAKDGRYEAVVYEQDPRSNARKLVSFEPI 116

[Close]

BLAST of Lag0038256 vs. ExPASy Swiss-Prot
Match: Q10Q46 (Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210200 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.9e-11
Identity = 39/91 (42.86%), Postives = 56/91 (61.54%), Query Frame = 0


Query: 24  PGSWEPIENIQDPYVQEMGRFAVMEHNKQIMEKMGANLEFICVVKGDKQVVAGVNYRLII 83
           P  W PI+NI DP++QE+GR+A+ E+N+         L F  V  G++QVV+G+NYRL I
Sbjct: 20  PRGWSPIKNIDDPHIQELGRWAITENNR---VSPSDELTFHRVTGGEQQVVSGMNYRLEI 79

Query: 84  EARN-EMKMVWSYEAIVNDRPWEKKWTLISF 114
           EA +    +  SY A+V ++ W     LISF
Sbjct: 80  EAASGGGDVTGSYGAVVFEQEWSNTRKLISF 107

The following BLAST results are available for this feature:

BLAST of Lag0038256 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897049.1	2.4e-43	75.63	cysteine proteinase inhibitor 5-like [Benincasa hispida]	[more]
XP_022975710.1	5.9e-34	63.03	cysteine proteinase inhibitor 1-like [Cucurbita maxima]	[more]
XP_022936213.1	1.7e-33	62.18	cysteine proteinase inhibitor 1-like [Cucurbita moschata]	[more]
XP_023536174.1	2.3e-33	62.18	cysteine proteinase inhibitor 8-like [Cucurbita pepo subsp. pepo]	[more]
KAG6592144.1	2.9e-33	61.34	Cysteine proteinase inhibitor 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lag0038256 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P86472	8.4e-15	46.88	Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841...	[more]
Q6TPK4	2.4e-14	45.83	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]
Q41916	1.2e-13	46.15	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=...	[more]
Q10Q47	3.9e-12	45.16	Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q10Q46	1.9e-11	42.86	Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 22..116 e-value: 3.2E-6 score: 36.7
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 30..114 e-value: 1.1E-24 score: 86.5
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 25..113 e-value: 2.98289E-11 score: 54.0172
None	No IPR available	GENE3D	3.10.450.10		coord: 21..117 e-value: 3.2E-26 score: 93.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..19
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR47364:SF2	CYSTEINE PROTEINASE INHIBITOR 5	coord: 24..114
None	No IPR available	PANTHER	PTHR47364	CYSTEINE PROTEINASE INHIBITOR 5	coord: 24..114
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 25..116
IPR018073	Proteinase inhibitor I25, cystatin, conserved site	PROSITE	PS00287	CYSTATIN	coord: 71..84

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0038256.1	Lag0038256.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity