Lag0038241 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0038241
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCalcium-binding EF-hand family protein
Locationchr2: 14173721 .. 14174239 (+)
RNA-Seq ExpressionLag0038241
SyntenyLag0038241
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCATTGTACCTACAATTGTCGATGTTGAATACTCGACCGATAATATGTCTTCATTGAACTCCTTCGATTTACAGAGGATTTTCAAAACGATCGACAAAAATGGCGACGGTTTCGTGAGTATCGAGGAACTCAATTGGCTGCTTAATGGTGTCGGCGTCGACCTAATTTTGGACGATCTCGAGAACTTGATAGGTAAATCGAAGATTGATTTCAATGAATTCTTGTCTCTGTACGAATCGTTATTGGAGAAAAATGATTCGGAGAACGGAGAAGGCGAAGAGGAAGAGGAGATTGAGAACGATCTTTTGAATGCATTTGAAGTGTTCGACGAAAATCGAGATGGATTTATTTCGAGCGATGAGCTTCGGAATGTGTTGGTGAGGTTGGGGCTTTGGGATGAGCGTTGTGGCAGTGATTGTAGTCGTATGATTGGCGCATTTGATACCAATTTGGATGGGCGACTAGATTTTGAGGAATTCAAGAACATGATGCGCTCTTCCAATTCTTCTTCTTAG

mRNA sequence

ATGGGCATTGTACCTACAATTGTCGATGTTGAATACTCGACCGATAATATGTCTTCATTGAACTCCTTCGATTTACAGAGGATTTTCAAAACGATCGACAAAAATGGCGACGGTTTCGTGAGTATCGAGGAACTCAATTGGCTGCTTAATGGTGTCGGCGTCGACCTAATTTTGGACGATCTCGAGAACTTGATAGGTAAATCGAAGATTGATTTCAATGAATTCTTGTCTCTGTACGAATCGTTATTGGAGAAAAATGATTCGGAGAACGGAGAAGGCGAAGAGGAAGAGGAGATTGAGAACGATCTTTTGAATGCATTTGAAGTGTTCGACGAAAATCGAGATGGATTTATTTCGAGCGATGAGCTTCGGAATGTGTTGGTGAGGTTGGGGCTTTGGGATGAGCGTTGTGGCAGTGATTGTAGTCGTATGATTGGCGCATTTGATACCAATTTGGATGGGCGACTAGATTTTGAGGAATTCAAGAACATGATGCGCTCTTCCAATTCTTCTTCTTAG

Coding sequence (CDS)

ATGGGCATTGTACCTACAATTGTCGATGTTGAATACTCGACCGATAATATGTCTTCATTGAACTCCTTCGATTTACAGAGGATTTTCAAAACGATCGACAAAAATGGCGACGGTTTCGTGAGTATCGAGGAACTCAATTGGCTGCTTAATGGTGTCGGCGTCGACCTAATTTTGGACGATCTCGAGAACTTGATAGGTAAATCGAAGATTGATTTCAATGAATTCTTGTCTCTGTACGAATCGTTATTGGAGAAAAATGATTCGGAGAACGGAGAAGGCGAAGAGGAAGAGGAGATTGAGAACGATCTTTTGAATGCATTTGAAGTGTTCGACGAAAATCGAGATGGATTTATTTCGAGCGATGAGCTTCGGAATGTGTTGGTGAGGTTGGGGCTTTGGGATGAGCGTTGTGGCAGTGATTGTAGTCGTATGATTGGCGCATTTGATACCAATTTGGATGGGCGACTAGATTTTGAGGAATTCAAGAACATGATGCGCTCTTCCAATTCTTCTTCTTAG

Protein sequence

MGIVPTIVDVEYSTDNMSSLNSFDLQRIFKTIDKNGDGFVSIEELNWLLNGVGVDLILDDLENLIGKSKIDFNEFLSLYESLLEKNDSENGEGEEEEEIENDLLNAFEVFDENRDGFISSDELRNVLVRLGLWDERCGSDCSRMIGAFDTNLDGRLDFEEFKNMMRSSNSSS
Homology
BLAST of Lag0038241 vs. NCBI nr
Match: XP_038899375.1 (calmodulin-like protein 3 [Benincasa hispida])

HSP 1 Score: 217.2 bits (552), Expect = 1.1e-52
Identity = 122/172 (70.93%), Postives = 136/172 (79.07%), Query Frame = 0

Query: 6   TIVDVEYSTDNMSSLNSFDLQRIFKTIDKNGDGFVSIEELNWLLNGVGVDLILDDLENLI 65
           +IVDVE S   MS LNS DLQRIF+TID+NGDGFVS+EELN LLN +GVDL  +DLE+L 
Sbjct: 46  SIVDVE-SLCTMSPLNSSDLQRIFETIDRNGDGFVSMEELNLLLNRIGVDLASEDLESLT 105

Query: 66  GKSKIDFNEFLSLYESLLEKNDSENGEGEEEEEIENDLLNAFEVFDENRDGFISSDELRN 125
           GKSKID NEFL LYES+ EK +   GE    EE+E+DLLNAF VFDENRDGFIS DEL N
Sbjct: 106 GKSKIDLNEFLCLYESISEKKE---GENRGSEEVESDLLNAFMVFDENRDGFISCDELEN 165

Query: 126 VLVRLGLW-DERC----GSDCSRMIGAFDTNLDGRLDFEEFKNMMRSSNSSS 173
           VLVRLGLW DERC    G  C RMIGAFD +LDGR+DF+EFKNMM SSNS S
Sbjct: 166 VLVRLGLWNDERCGGCGGDYCRRMIGAFDMDLDGRIDFQEFKNMMNSSNSPS 213

BLAST of Lag0038241 vs. NCBI nr
Match: XP_022976028.1 (probable calcium-binding protein CML44 [Cucurbita maxima])

HSP 1 Score: 216.5 bits (550), Expect = 1.8e-52
Identity = 116/157 (73.89%), Postives = 128/157 (81.53%), Query Frame = 0

Query: 17  MSSLNSFDLQRIFKTIDKNGDGFVSIEELNWLLNGVGVDLILDDLENLIGKSKIDFNEFL 76
           MS  N+ DLQRIF+TID+NGDGFVSIEEL+WLLN  GVD I DDLE+LIGKSKIDF+EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSKIDFDEFL 60

Query: 77  SLYESLLEKNDSENGEGEEEEEIENDLLNAFEVFDENRDGFISSDELRNVLVRLGLWDE- 136
           SLYES+ E N          EEIE+DLLNAF+VFDENRDGFIS DEL NVLVRLGLWD+ 
Sbjct: 61  SLYESISEIN-------RGGEEIESDLLNAFQVFDENRDGFISCDELHNVLVRLGLWDDK 120

Query: 137 RCGSDCSRMIGAFDTNLDGRLDFEEFKNMMRSSNSSS 173
           RC  DC RMIGAFD +LDGRLDFEEFKNMM  S+ SS
Sbjct: 121 RCVRDCRRMIGAFDVDLDGRLDFEEFKNMMNYSSHSS 150

BLAST of Lag0038241 vs. NCBI nr
Match: KAG7025009.1 (Calcium-binding protein CML42, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 216.5 bits (550), Expect = 1.8e-52
Identity = 118/158 (74.68%), Postives = 129/158 (81.65%), Query Frame = 0

Query: 17  MSSLNSFDLQRIFKTIDKNGDGFVSIEELNWLLNGVGVDLILDDLENLIGKSKIDFNEFL 76
           MS  N+ DLQRIF+TID+NGDGFVSIEEL+WLLN  GVD I DDLE+LIGKS IDF+EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSMIDFDEFL 60

Query: 77  SLYESLLEKNDSENGEGEEEEEIENDLLNAFEVFDENRDGFISSDELRNVLVRLGLWDE- 136
           SLYES+ E N          EEIE+DLLNAF+VFDENRDGFIS DEL NVLVRLGLWD+ 
Sbjct: 61  SLYESISEMN-------RGGEEIESDLLNAFQVFDENRDGFISCDELHNVLVRLGLWDDR 120

Query: 137 RCGSDCSRMIGAFDTNLDGRLDFEEFKNMMR-SSNSSS 173
           RC  DC RMIGAFD +LDGRLDFEEFKNMM  SSNSSS
Sbjct: 121 RCVRDCRRMIGAFDVDLDGRLDFEEFKNMMNYSSNSSS 151

BLAST of Lag0038241 vs. NCBI nr
Match: XP_023536413.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 214.9 bits (546), Expect = 5.3e-52
Identity = 115/157 (73.25%), Postives = 128/157 (81.53%), Query Frame = 0

Query: 17  MSSLNSFDLQRIFKTIDKNGDGFVSIEELNWLLNGVGVDLILDDLENLIGKSKIDFNEFL 76
           MS  N+ DLQRIF+TID+NGDGFVSIEEL+WLLN  GVD I DDLE+LIGKS IDF+EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSMIDFDEFL 60

Query: 77  SLYESLLEKNDSENGEGEEEEEIENDLLNAFEVFDENRDGFISSDELRNVLVRLGLWDE- 136
           SLYES+ E N          EEIE+DLLNAF+VFDENRDGFIS DEL NVLVRLGLWD+ 
Sbjct: 61  SLYESISEIN-------RGGEEIESDLLNAFQVFDENRDGFISCDELHNVLVRLGLWDDR 120

Query: 137 RCGSDCSRMIGAFDTNLDGRLDFEEFKNMMRSSNSSS 173
           RC  DC RMIGAFD +LDGRLDFEEFKNMM  S++SS
Sbjct: 121 RCVRDCRRMIGAFDVDLDGRLDFEEFKNMMNYSSNSS 150

BLAST of Lag0038241 vs. NCBI nr
Match: KAG6592136.1 (Calcium-binding protein CML42, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 214.5 bits (545), Expect = 7.0e-52
Identity = 117/158 (74.05%), Postives = 128/158 (81.01%), Query Frame = 0

Query: 17  MSSLNSFDLQRIFKTIDKNGDGFVSIEELNWLLNGVGVDLILDDLENLIGKSKIDFNEFL 76
           MS  N+ DLQRIF+TID+NGDGFVSIEEL+WLLN  GVD I DDLE+LIGKS IDF+EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSMIDFDEFL 60

Query: 77  SLYESLLEKNDSENGEGEEEEEIENDLLNAFEVFDENRDGFISSDELRNVLVRLGLWDE- 136
           SLYES+ E N          EEIE+DLLNAF+VFDEN DGFIS DEL NVLVRLGLWD+ 
Sbjct: 61  SLYESISEMN-------RGGEEIESDLLNAFQVFDENHDGFISCDELHNVLVRLGLWDDK 120

Query: 137 RCGSDCSRMIGAFDTNLDGRLDFEEFKNMMR-SSNSSS 173
           RC  DC RMIGAFD +LDGRLDFEEFKNMM  SSNSSS
Sbjct: 121 RCVRDCRRMIGAFDVDLDGRLDFEEFKNMMNYSSNSSS 151

BLAST of Lag0038241 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 1.1e-26
Identity = 69/151 (45.70%), Postives = 99/151 (65.56%), Query Frame = 0

Query: 18  SSLNSFDLQRIFKTIDKNGDGFVSIEELNWLLNGVG-VDLILDDLENLIGKSKIDFNEFL 77
           S + + DL+R+FKT+DKN DG V+++EL W+L+ +G  +   D+LE ++GK  +D +EFL
Sbjct: 4   SFITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFL 63

Query: 78  SLYESLLEKNDSENGEGEEEEEIEND--LLNAFEVFDENRDGFISSDELRNVLVRLGLWD 137
             Y   +   DS+  +   +   +ND  +  AF VFD N DG+IS++ELR+VL RLG  +
Sbjct: 64  RFYYDAV--LDSKGSKKNIDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGFEE 123

Query: 138 ERCGSDCSRMIGAFDTNLDGRLDFEEFKNMM 166
           E    DC RMI   D NLDG +DFEEFKNM+
Sbjct: 124 EAKAWDCGRMIRVHDKNLDGFVDFEEFKNMI 152

BLAST of Lag0038241 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.3e-24
Identity = 71/162 (43.83%), Postives = 103/162 (63.58%), Query Frame = 0

Query: 19  SLNSFDLQRIFKTIDKNGDGFVSIEELNWLLNGVGVDLILDDLENLI------GKSKIDF 78
           SLN+  LQRIF   DKNGDGF+++EEL+  L  +G++  L DL++ +      G + ++F
Sbjct: 24  SLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNF 83

Query: 79  NEFLSLYESLLEK---NDSENGEGEEE----EEIENDLLNAFEVFDENRDGFISSDELRN 138
           ++F SL+++L +         GE E++     E E+DL  AF+VFDEN DGFIS+ EL+ 
Sbjct: 84  DDFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQT 143

Query: 139 VLVRLGLWDERCGSDCSRMIGAFDTNLDGRLDFEEFKNMMRS 168
           VL +LGL +        +MI + D N DGR+DF EFKNMMR+
Sbjct: 144 VLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRT 185

BLAST of Lag0038241 vs. ExPASy Swiss-Prot
Match: Q0JC44 (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 GN=CML22 PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.2e-22
Identity = 66/151 (43.71%), Postives = 90/151 (59.60%), Query Frame = 0

Query: 24  DLQRIFKTIDKNGDGFVSIEELNWLLNGVGVDLILDDLENLI------GKSKIDFNEFLS 83
           +L R+F+  D+NGDG ++ EEL   L  +G+ +  D+L  +I      G   +D  EF  
Sbjct: 92  ELARVFELFDRNGDGRITREELEDSLGKLGIPVPADELAAVIARIDANGDGCVDVEEFGE 151

Query: 84  LYESLLE-KNDSENGEG-EEEEEIENDLLNAFEVFDENRDGFISSDELRNVLVRLGLWDE 143
           LY S++   +DS++G   EEEEE + D+  AF VFD N DG+I+ DEL  VL  LGL   
Sbjct: 152 LYRSIMAGGDDSKDGRAKEEEEEEDGDMREAFRVFDANGDGYITVDELGAVLASLGLKQG 211

Query: 144 RCGSDCSRMIGAFDTNLDGRLDFEEFKNMMR 167
           R   +C RMIG  D + DGR+DF EF  MMR
Sbjct: 212 RTAEECRRMIGQVDRDGDGRVDFHEFLQMMR 242

BLAST of Lag0038241 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.6e-22
Identity = 61/153 (39.87%), Postives = 89/153 (58.17%), Query Frame = 0

Query: 20  LNSFDLQRIFKTIDKNGDGFVSIEELNWLLNGVGVDLILDDLENLI------GKSKIDFN 79
           ++  +L RIF+  D+NGDG ++ +ELN  L  +G+ +   DL  +I      G   +D  
Sbjct: 1   MDQAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIE 60

Query: 80  EFLSLYESLLEKNDSENGEGEEEEEIENDLLNAFEVFDENRDGFISSDELRNVLVRLGLW 139
           EF  LY++++E+ D            E D+  AF VFD+NRDGFI+ +ELR+VL  LGL 
Sbjct: 61  EFGGLYQTIMEERDE-----------EEDMREAFNVFDQNRDGFITVEELRSVLASLGLK 120

Query: 140 DERCGSDCSRMIGAFDTNLDGRLDFEEFKNMMR 167
             R   DC RMI   D + DG ++F+EFK MM+
Sbjct: 121 QGRTLEDCKRMISKVDVDGDGMVNFKEFKQMMK 142

BLAST of Lag0038241 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.0e-21
Identity = 58/149 (38.93%), Postives = 86/149 (57.72%), Query Frame = 0

Query: 24  DLQRIFKTIDKNGDGFVSIEELNWLLNGVGVDLILDDLENLIGKSKI------DFNEFLS 83
           +L R+F+  DKNGDG ++  EL      VG+ +  +++  +I K  +      D +EF S
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 84  LYESLLEKNDSENGEGEEEEEIENDLLNAFEVFDENRDGFISSDELRNVLVRLGLWDERC 143
           LY+ ++           EE+E E D+  AF VFD+N DGFI+ +ELR+VL  +GL   R 
Sbjct: 65  LYQEMV-----------EEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRT 124

Query: 144 GSDCSRMIGAFDTNLDGRLDFEEFKNMMR 167
             DC +MI   D + DG ++F+EFK MMR
Sbjct: 125 LEDCKKMISKVDVDGDGMVNFKEFKQMMR 142

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899375.11.1e-5270.93calmodulin-like protein 3 [Benincasa hispida][more]
XP_022976028.11.8e-5273.89probable calcium-binding protein CML44 [Cucurbita maxima][more]
KAG7025009.11.8e-5274.68Calcium-binding protein CML42, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_023536413.15.3e-5273.25probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo][more]
KAG6592136.17.0e-5274.05Calcium-binding protein CML42, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9LPK51.1e-2645.70Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ... [more]
Q9SVG91.3e-2443.83Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1[more]
Q0JC441.2e-2243.71Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9SRR71.6e-2239.87Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1[more]
Q9SU003.0e-2138.93Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 140..168
e-value: 0.0027
score: 26.9
coord: 24..52
e-value: 1.7E-4
score: 30.9
coord: 102..130
e-value: 9.0E-5
score: 31.8
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 101..166
e-value: 1.8E-9
score: 37.9
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 25..45
e-value: 1.7E-6
score: 27.2
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 20..55
score: 13.760229
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 98..133
score: 14.290241
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 136..171
score: 10.914899
IPR002048EF-hand domainCDDcd00051EFhcoord: 102..166
e-value: 2.88581E-11
score: 54.0909
NoneNo IPR availableGENE3D1.10.238.10coord: 7..92
e-value: 1.6E-10
score: 43.1
NoneNo IPR availableGENE3D1.10.238.10coord: 97..172
e-value: 5.3E-19
score: 70.1
NoneNo IPR availablePANTHERPTHR10891:SF788CALCIUM-BINDING PROTEIN CML44-RELATEDcoord: 17..170
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 17..170
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 33..45
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 111..123
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 25..166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0038241.1Lag0038241.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding