Lag0038082 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0038082
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	MBOAT (membrane bound O-acyl transferase) family protein
Location	chr2: 12369719 .. 12370753 (-)
RNA-Seq Expression	Lag0038082
Synteny	Lag0038082

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGGCGAAATCCTAGGATTCATCAAGGTGTTGCTCATATTCATGGTGTCTTTGTTGTATTGCTATTTCATAGCTTCAAAACTTCCCAAAGGCAAACTCAGGCTCCTCTCTCTCCTTCCCGTCTTCTCGCTCTTCGTCGTCCTCCCTCTCTACCTCTCTTCCATTCTCCTCACGAGCGCTATCGCCTTCTTCGTCACTTGGCTTACGACCTTTAAGCTCCTTCTATTCTCATTCGATTTAGGTCCTCTAGCTTCGGACCCACCATTGCCATTCCCTCTTTTCGCCTCCATTGCTTTCTTTCCAACAAGAGTCAAGCATAACAAAACAACCCCATATGCTCAAAACCTTAGAAAAGATCATTCAAATGCACCTGAAGAAGTTGGCCAATTAGAGCCACCTCCCAAGTTGCCTCTAAACATGCCAGCCAAAGTGGTGATACTTGCCATATTGGTGTTGGGCAAGAGTTACTACATGGTCGAGCTCGTCAGTCGTCCCATTGCAAAGCTATCCATCAATTGTGCAATGTTGTGTTTCTACTTAGACATGGCTACGAGTCTCTCCAATGCTTTTGTCCAATCTACTTTCGGCGTTGAGGTCAAACGGCCATTCATTGAGCCGTATCTCGCAACGTCGCTTCAAAATTTCTGGGGTCGGAGGTGGAATCTATTGGTATCTGAAACTTTGCACGACACGATCTATAAACCAACTCGTTACAAAGCAAGGAGGCCGCGATGGGCGGCTGTGATGGTAGTGTTTGTGGTGTCAGGGTTGATGCACGAGCTTTGGTATTACTACCTCGTCAGAGCGATTCCGACGTGGGAAGCCACATGGTACTTTGTCATTCATGGGGTGTGTGTGGCTGTGGAAATCGAATTGAAGAGGACCTTGGGGAAAAGGTGGCAGTTGCACTCGATAGTGTCCAGACCACTAACGATAGCGTTTCTTGTGGTTACTTCCTATTGGTTATTATTTCCACTCATGATGAGGATAGACACATTCCCTCACATGGAAGATGAAATATTCCCCTATTGA

mRNA sequence

ATGGGTGGCGAAATCCTAGGATTCATCAAGGTGTTGCTCATATTCATGGTGTCTTTGTTGTATTGCTATTTCATAGCTTCAAAACTTCCCAAAGGCAAACTCAGGCTCCTCTCTCTCCTTCCCGTCTTCTCGCTCTTCGTCGTCCTCCCTCTCTACCTCTCTTCCATTCTCCTCACGAGCGCTATCGCCTTCTTCGTCACTTGGCTTACGACCTTTAAGCTCCTTCTATTCTCATTCGATTTAGGTCCTCTAGCTTCGGACCCACCATTGCCATTCCCTCTTTTCGCCTCCATTGCTTTCTTTCCAACAAGAGTCAAGCATAACAAAACAACCCCATATGCTCAAAACCTTAGAAAAGATCATTCAAATGCACCTGAAGAAGTTGGCCAATTAGAGCCACCTCCCAAGTTGCCTCTAAACATGCCAGCCAAAGTGGTGATACTTGCCATATTGGTGTTGGGCAAGAGTTACTACATGGTCGAGCTCGTCAGTCGTCCCATTGCAAAGCTATCCATCAATTGTGCAATGTTGTGTTTCTACTTAGACATGGCTACGAGTCTCTCCAATGCTTTTGTCCAATCTACTTTCGGCGTTGAGGTCAAACGGCCATTCATTGAGCCGTATCTCGCAACGTCGCTTCAAAATTTCTGGGGTCGGAGGTGGAATCTATTGGTATCTGAAACTTTGCACGACACGATCTATAAACCAACTCGTTACAAAGCAAGGAGGCCGCGATGGGCGGCTGTGATGGTAGTGTTTGTGGTGTCAGGGTTGATGCACGAGCTTTGGTATTACTACCTCGTCAGAGCGATTCCGACGTGGGAAGCCACATGGTACTTTGTCATTCATGGGGTGTGTGTGGCTGTGGAAATCGAATTGAAGAGGACCTTGGGGAAAAGGTGGCAGTTGCACTCGATAGTGTCCAGACCACTAACGATAGCGTTTCTTGTGGTTACTTCCTATTGGTTATTATTTCCACTCATGATGAGGATAGACACATTCCCTCACATGGAAGATGAAATATTCCCCTATTGA

Coding sequence (CDS)

ATGGGTGGCGAAATCCTAGGATTCATCAAGGTGTTGCTCATATTCATGGTGTCTTTGTTGTATTGCTATTTCATAGCTTCAAAACTTCCCAAAGGCAAACTCAGGCTCCTCTCTCTCCTTCCCGTCTTCTCGCTCTTCGTCGTCCTCCCTCTCTACCTCTCTTCCATTCTCCTCACGAGCGCTATCGCCTTCTTCGTCACTTGGCTTACGACCTTTAAGCTCCTTCTATTCTCATTCGATTTAGGTCCTCTAGCTTCGGACCCACCATTGCCATTCCCTCTTTTCGCCTCCATTGCTTTCTTTCCAACAAGAGTCAAGCATAACAAAACAACCCCATATGCTCAAAACCTTAGAAAAGATCATTCAAATGCACCTGAAGAAGTTGGCCAATTAGAGCCACCTCCCAAGTTGCCTCTAAACATGCCAGCCAAAGTGGTGATACTTGCCATATTGGTGTTGGGCAAGAGTTACTACATGGTCGAGCTCGTCAGTCGTCCCATTGCAAAGCTATCCATCAATTGTGCAATGTTGTGTTTCTACTTAGACATGGCTACGAGTCTCTCCAATGCTTTTGTCCAATCTACTTTCGGCGTTGAGGTCAAACGGCCATTCATTGAGCCGTATCTCGCAACGTCGCTTCAAAATTTCTGGGGTCGGAGGTGGAATCTATTGGTATCTGAAACTTTGCACGACACGATCTATAAACCAACTCGTTACAAAGCAAGGAGGCCGCGATGGGCGGCTGTGATGGTAGTGTTTGTGGTGTCAGGGTTGATGCACGAGCTTTGGTATTACTACCTCGTCAGAGCGATTCCGACGTGGGAAGCCACATGGTACTTTGTCATTCATGGGGTGTGTGTGGCTGTGGAAATCGAATTGAAGAGGACCTTGGGGAAAAGGTGGCAGTTGCACTCGATAGTGTCCAGACCACTAACGATAGCGTTTCTTGTGGTTACTTCCTATTGGTTATTATTTCCACTCATGATGAGGATAGACACATTCCCTCACATGGAAGATGAAATATTCCCCTATTGA

Protein sequence

MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTSAIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKDHSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLCFYLDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYKARRPRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKRWQLHSIVSRPLTIAFLVVTSYWLLFPLMMRIDTFPHMEDEIFPY

Homology

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BLAST of Lag0038082 vs. NCBI nr
Match: XP_038899290.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Benincasa hispida])

HSP 1 Score: 455.7 bits (1171), Expect = 3.6e-124
Identity = 239/335 (71.34%), Postives = 271/335 (80.90%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           M GEI+ FIKVLLI M SLLYCY IASKLPKGKLRLLSLLP+F LFVVLPL LS ++LTS
Sbjct: 1   MDGEIIRFIKVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFPLFVVLPLDLSYVVLTS 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKD 120
            I  FVTWLTTFKLL FSFDLGPLASDPPL FPLFASIAF PTR+K  KTTP  QN RKD
Sbjct: 61  GITIFVTWLTTFKLLQFSFDLGPLASDPPLSFPLFASIAFLPTRIKDIKTTPSVQNHRKD 120

Query: 121 HSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLCFY 180
            SN P+E+ Q EPPPKLPLN+PAKV + A+LV GKSY  V+LVS PI KL  NC +L FY
Sbjct: 121 -SNTPQELDQ-EPPPKLPLNLPAKVGLFALLVFGKSY--VDLVS-PIVKLCFNCGILYFY 180

Query: 181 LDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYK 240
           LDM  SLSN FV+S+FGVEV+R F EPYLATSLQNFWGRRWN LVSETLH+TIYKP RY 
Sbjct: 181 LDMVMSLSNIFVRSSFGVEVRRSFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVRYD 240

Query: 241 ARRPRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 300
              PRW AV VVFVVSGLMHEL +YY++R +PTWE TW+F++HGVCVA EIELK  LGK+
Sbjct: 241 VGSPRWMAVFVVFVVSGLMHELLFYYVIRVVPTWEVTWFFIVHGVCVAAEIELKSALGKK 300

Query: 301 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMRIDTFP 336
           W  H  V+ PLT+AF+ VT++WL FP ++R+   P
Sbjct: 301 WHFHGAVTGPLTMAFMGVTAHWLFFPQLLRMKYSP 330

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BLAST of Lag0038082 vs. NCBI nr
Match: XP_022936880.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 440.3 bits (1131), Expect = 1.6e-119
Identity = 228/330 (69.09%), Postives = 266/330 (80.61%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           MG E++ F+KV+LI M SLLYCYFIA KLPKGK RL+S LPVFSLF+VLPLY+SS  L+S
Sbjct: 1   MGSELISFMKVILISMASLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSAFLSS 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKD 120
           + AF VTWLTTFKL+LFSF+LGPLA D  L FPLFASIAF PTR+KH+KT  Y QN    
Sbjct: 61  SFAFLVTWLTTFKLVLFSFNLGPLAFDSKLSFPLFASIAFLPTRIKHSKTAKYTQN---- 120

Query: 121 HSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLCFY 180
                EEVG+ EP PK+PLN+PAKVVI  +L++GK YY  E+V  PIAKL +NCA L FY
Sbjct: 121 ----QEEVGK-EPRPKIPLNLPAKVVIFVMLIMGKKYY--EIVD-PIAKLCMNCATLYFY 180

Query: 181 LDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYK 240
           LDM   LSN FV+S FGVEV RPF EPYLATSLQNFWGRRWN LVSETLH+ +Y+P RY+
Sbjct: 181 LDMIMCLSNVFVRSRFGVEVSRPFDEPYLATSLQNFWGRRWNRLVSETLHNAVYQPVRYE 240

Query: 241 ARRPRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 300
              PRW AV+VVFVVSGLMHEL  YY++RAIPTWE TW+FVIHG+CVAVEIELKR LGKR
Sbjct: 241 VGGPRWMAVVVVFVVSGLMHELLLYYIIRAIPTWEVTWFFVIHGLCVAVEIELKRALGKR 300

Query: 301 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMR 331
           W++H  VS PLT+AFLV T++WL FP ++R
Sbjct: 301 WRIHWAVSGPLTMAFLVATAHWLFFPPLLR 318

[Close]

BLAST of Lag0038082 vs. NCBI nr
Match: XP_023536076.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 439.1 bits (1128), Expect = 3.5e-119
Identity = 228/330 (69.09%), Postives = 265/330 (80.30%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           MG E++   KV+LI M SLLYCYFIA KLPKGK RL+S LPVFSLF+VLPLY+SS  L+S
Sbjct: 1   MGSELISLTKVILISMASLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSAFLSS 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKD 120
           + AF VTWLTTFKL+LFSF+LGPLA D  L FPLFASIAF PTR+KH+KT  YAQN    
Sbjct: 61  SFAFLVTWLTTFKLVLFSFNLGPLAFDSKLSFPLFASIAFLPTRIKHSKTAKYAQN---- 120

Query: 121 HSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLCFY 180
                EEV + EP PK+PLN+PAKVVI  +L++GK YY  E+V  PIAKL +NCA L FY
Sbjct: 121 ----QEEVDK-EPRPKIPLNLPAKVVIFVMLIMGKKYY--EIVD-PIAKLCMNCATLYFY 180

Query: 181 LDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYK 240
           LDM   LSN FV+S FGVEV RPF EPYLATSLQNFWGRRWN LVSETLH+ +Y+P RY+
Sbjct: 181 LDMIMCLSNVFVRSRFGVEVSRPFDEPYLATSLQNFWGRRWNRLVSETLHNAVYQPVRYE 240

Query: 241 ARRPRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 300
              PRW AV+VVFVVSGLMHEL  YY++RAIPTWE TW+FVIHG+CVAVEIELKR LGKR
Sbjct: 241 VGGPRWMAVVVVFVVSGLMHELLLYYIIRAIPTWEVTWFFVIHGLCVAVEIELKRALGKR 300

Query: 301 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMR 331
           WQ+H +VS PLT+AFLV T++WL FP ++R
Sbjct: 301 WQIHWVVSGPLTMAFLVATAHWLFFPPLLR 318

[Close]

BLAST of Lag0038082 vs. NCBI nr
Match: XP_022976365.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima])

HSP 1 Score: 435.6 bits (1119), Expect = 3.9e-118
Identity = 226/330 (68.48%), Postives = 263/330 (79.70%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           MG E++  +KV+LI M SLLYCYFIA KLPKGK RL+S LPVFSLF+VLPLY+SS  L+S
Sbjct: 1   MGSELISLMKVILISMASLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSAFLSS 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKD 120
           + AF VTWLTTFKL+LFSF+LGPLA D  L FPLFASIAF PTR+KH+KT  Y QN    
Sbjct: 61  SFAFIVTWLTTFKLVLFSFNLGPLAFDSKLSFPLFASIAFLPTRIKHSKTAKYVQN---- 120

Query: 121 HSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLCFY 180
                EEVG+ EP PK+PLN+PAKVVI  +L++GK YY  E+V  PIAK  +NCAML FY
Sbjct: 121 ----QEEVGK-EPHPKIPLNLPAKVVIFVMLIMGKKYY--EIVD-PIAKSCMNCAMLYFY 180

Query: 181 LDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYK 240
           LDM   LSN FV+S FGVEV RPF EPYLATSLQNFWGRRWN LVSETLH+ +Y+P RY+
Sbjct: 181 LDMIMCLSNVFVRSRFGVEVSRPFNEPYLATSLQNFWGRRWNRLVSETLHNAVYQPVRYE 240

Query: 241 ARRPRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 300
              PRW AV+VVFVVSGLMHEL  YY++R IPTWE TW+FVIHG+CVAVEIELKR LGKR
Sbjct: 241 VGGPRWMAVVVVFVVSGLMHELLLYYIIRVIPTWEVTWFFVIHGLCVAVEIELKRALGKR 300

Query: 301 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMR 331
           W++H  VS PLT  FLV T++WLLFP ++R
Sbjct: 301 WRIHWAVSGPLTTVFLVATAHWLLFPPLLR 318

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BLAST of Lag0038082 vs. NCBI nr
Match: XP_004151023.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis sativus] >KGN44004.1 hypothetical protein Csa_016501 [Cucumis sativus])

HSP 1 Score: 435.3 bits (1118), Expect = 5.0e-118
Identity = 227/335 (67.76%), Postives = 268/335 (80.00%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           M GE++  +KVLLI M SL+YCY+IASKLPKGK RLLSLLP+FSLF VLPL LS ++LTS
Sbjct: 3   MDGEVIRLMKVLLICMASLMYCYYIASKLPKGKPRLLSLLPIFSLFAVLPLDLSYVVLTS 62

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKD 120
            I  FVTWLTTFKL+ FSFDLGPLASDPPL FPLFASIAF PTR+K  KTTPY QN +KD
Sbjct: 63  GITIFVTWLTTFKLIQFSFDLGPLASDPPLSFPLFASIAFLPTRIKDIKTTPYVQNHKKD 122

Query: 121 HSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLCFY 180
            SNAP+E+ Q E PPKLPLN+PAK  I A+LV+ K++  V LV  PI KL +NC +L FY
Sbjct: 123 -SNAPQELDQ-ETPPKLPLNLPAKAFIFALLVICKNH--VHLV-HPILKLILNCGILYFY 182

Query: 181 LDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYK 240
           LDM  S+SN FV+ +FG+EV+R F EPYLATSLQNFWGRRWN LVSETLH+ IYKP RY 
Sbjct: 183 LDMIMSISNEFVRLSFGIEVRRSFDEPYLATSLQNFWGRRWNRLVSETLHNMIYKPIRYD 242

Query: 241 ARRPRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 300
              PRW AV VVFVVSGLMHEL +YY++R +PTWE TW+FVIHGV VA+EIELK  LGK+
Sbjct: 243 VGSPRWMAVFVVFVVSGLMHELLFYYIIRVVPTWEVTWFFVIHGVFVALEIELKSALGKK 302

Query: 301 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMRIDTFP 336
           WQ H  V+ PLT+AF+ VT++WL FP ++R+   P
Sbjct: 303 WQFHWAVTGPLTMAFMAVTAHWLFFPQLLRMKYSP 332

[Close]

BLAST of Lag0038082 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 4.7e-50
Identity = 131/339 (38.64%), Postives = 189/339 (55.75%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           M  E+  FIK+ +  ++S+ YCY++++ +  G  RLLS+LPV +LF+V PL+ S +  + 
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPL--FASIAFFPTRVKHNKTTPYAQNLR 120
            +AFF++WL  FKL+LFSFD GPL+   PLP  L  F  I  FP + + N   P  QN  
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIKPQQN---PNIQNY- 120

Query: 121 KDHSNAPEEVGQLEPPPKLPLNMPA-KVVILAILVLGKSYYMVELVSRPIAKLSINCAML 180
                            K+P+ + A KVVI  +L+     Y  +    P   L  N   +
Sbjct: 121 -----------------KIPIWLFAIKVVIFVVLL---QMYEYKQYLSPALLLVFNSLHI 180

Query: 181 CFYLDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPT 240
              L++   L  A V  T G +++    EPYLATSLQ+FWGRRWNL+V   L   +Y P 
Sbjct: 181 FLELEIVFMLVKALVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPA 240

Query: 241 RYKARR------PRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEI 300
           R  A R        +  V   F+VSG +HE+ ++YL R +PT E TW+F++HGVC   E+
Sbjct: 241 RRMACRKVNSDQAMFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEV 300

Query: 301 ELKRTLGKR--WQLHSIVSRPLTIAFLVVTSYWLLFPLM 329
            +K++   R  W++   VSR LT+ F+VVTS W  FPL+
Sbjct: 301 AVKKSTFVRRWWRVSPTVSRLLTVGFVVVTSGWFFFPLI 312

[Close]

BLAST of Lag0038082 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 5.2e-49
Identity = 132/340 (38.82%), Postives = 189/340 (55.59%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           M  E+   IKV +  ++S+ YCY+I  ++  G  R LS+ PV +LF+VLPL+ SS+ L+ 
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLP--FPLFASIAFFPTRVKHNKTTPYAQNLR 120
             AFF+TWL  FKL+LFSFD GPL    P+P  FP F     FP +V+ N   P +QN  
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLI---PIPTNFPRFFCFTCFPIKVQQN---PKSQN-- 120

Query: 121 KDHSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLC 180
                           PKL   +  K+ I A+L+     Y       P   L +    L 
Sbjct: 121 --------------HLPKLVFAI--KLAIFAVLL---HLYSYRQNLSPTILLGLYFVHLY 180

Query: 181 FYLDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTR 240
             +++  +     V  + G +++    +PYLATSLQ+FWGRRWNL+V   L   +Y P R
Sbjct: 181 LEIEIILTFVKVVVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMR 240

Query: 241 YKARRPR---WA---AVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIE 300
             + R     WA    ++  F+VSGL+HEL ++YL+R +PT E T +FV+HGVC AVE+ 
Sbjct: 241 RVSERKMSSGWALFPGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELA 300

Query: 301 LKR--TLGKRWQLHSIVSRPLTIAFLVVTSYWLLFPLMMR 331
           +K+  T+ +RW+L   VSR LT+ F+ VT  WL  P + R
Sbjct: 301 VKKKTTVAQRWRLSPGVSRVLTVGFVFVTGGWLFTPQLKR 313

[Close]

BLAST of Lag0038082 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.1e-48
Identity = 134/330 (40.61%), Postives = 183/330 (55.45%), Query Frame = 0


Query: 9   IKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTSAIAFFVTW 68
           I+V +  ++SL YCY+I+SKL KG LRLLS+LPV  LF+VLPL+LS +   +    F++W
Sbjct: 9   IEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLSW 68

Query: 69  LTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKDHSNAPEEV 128
           L  FKLLLF+FD GPL    PLP  L   I F    +K          +R+D S  P  +
Sbjct: 69  LANFKLLLFAFDEGPLF---PLPPKLSRFICFACLPIK----------IRQDPS--PNAI 128

Query: 129 GQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVS--RPIAKLSINCAMLCFYLDMATS 188
             L P P     MP  V+ + ILVLG   ++ E          L++ C  +   +++   
Sbjct: 129 PNLHPKP-----MPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLV 188

Query: 189 LSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYKARR--- 248
              A V +  G  ++  F EPYLATSLQ+FW RRWNL+VS  L  T++ P +   +R   
Sbjct: 189 FVGAVVSTLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILS 248

Query: 249 ---PRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 308
                +A VM  F VSGLMHEL Y+Y++R  PTWE T +FV+HG   A EI +KRT   R
Sbjct: 249 PDGAMFAGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQWLR 308

Query: 309 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMR 331
              H  VS  + + F+ VT  WL    ++R
Sbjct: 309 -PPHRAVSGLVVLTFVSVTGVWLFLAQVLR 317

[Close]

BLAST of Lag0038082 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 3.3e-48
Identity = 119/340 (35.00%), Postives = 194/340 (57.06%), Query Frame = 0


Query: 8   FIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTSAIAFFVT 67
           FIK   + ++SL Y +FIA  +PKG  RL+   PVF +F ++P  + S+ L    AFF+ 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLTTFKLLLFSFDLGPLASD-PPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKDHSNAPE 127
           WL  FKLLLF+   GPL+S+  PL  P+F +++  P +++       +    K HS+   
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQ------LSPKPTKTHSHEGS 123

Query: 128 EVGQLEPPPKLPLNMPAKVVILAILVLGKSY-YMVELVSRPIAKLSINCAMLCFYLDMAT 187
             G        PL    K V + +++  K+Y Y  +L  + +  L++    + F L++  
Sbjct: 124 TEG--------PLIYTIKAVFVVLII--KAYEYSTKLPEKVV--LTLYAIHIYFALEIIL 183

Query: 188 SLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPT--RYKARR 247
           + + A V++   +E++  F +PYLATSLQ+FWGRRWNL+V+  L  T+Y+P+   +    
Sbjct: 184 AATAAAVRAMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLG 243

Query: 248 PRWAAVMV---VFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 307
           P ++ ++     FVVSG+MHEL ++Y+ R  P W+  W+F+I+G C  VEI +K+T+  R
Sbjct: 244 PNYSQILAAFGTFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGR 303

Query: 308 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMRIDTFPHMEDE 341
           W+    +S+ LT+ F++VT+ WL  P   R +      DE
Sbjct: 304 WRFPKAISQVLTLTFVMVTALWLFLPEFNRCNIVEKALDE 325

[Close]

BLAST of Lag0038082 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.7e-47
Identity = 127/353 (35.98%), Postives = 186/353 (52.69%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           M  E++  IKV +  ++S+ YCY+ ++++  G  RLLS+LPV  LF+VLPL++SS+  + 
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPLFASIAF--FPTRVKHNKTTPYAQNLR 120
           + AFF++WL  FKL+LFSFD GPL    P+P  L   + F  FP +++ N          
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLF---PVPSNLSRFVCFTCFPIKLQQN---------- 120

Query: 121 KDHSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLC 180
                           PK    MP     + +   G   +M E        + +    L 
Sbjct: 121 ----------------PKPQNQMPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLH 180

Query: 181 FYLDMATSLS--NAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKP 240
            YL+    L+     +  +   +++  F EPYL+TSLQ+FWGRRWNL+V   L   +Y P
Sbjct: 181 IYLEYEILLAPLKVLLSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLP 240

Query: 241 TRYKARRPR------WAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVE 300
            R  A R        +  V   F+VSG++HEL ++Y  R  PT E T +FV+HGVC A E
Sbjct: 241 VRQMAGRKMNSDQALFLGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAE 300

Query: 301 IELKRT-LGKRWQLHSIVSRPLTIAFLVVTSYWLLFPLMMRIDTFPHMEDEIF 343
              KRT L +RW++  +VSR LT+ F+V+T  WL FP + R      + DE F
Sbjct: 301 CAAKRTRLVRRWKVSQMVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAF 324

[Close]

BLAST of Lag0038082 vs. ExPASy TrEMBL
Match: A0A6J1FEY7 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111443337 PE=3 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 7.6e-120
Identity = 228/330 (69.09%), Postives = 266/330 (80.61%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           MG E++ F+KV+LI M SLLYCYFIA KLPKGK RL+S LPVFSLF+VLPLY+SS  L+S
Sbjct: 1   MGSELISFMKVILISMASLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSAFLSS 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKD 120
           + AF VTWLTTFKL+LFSF+LGPLA D  L FPLFASIAF PTR+KH+KT  Y QN    
Sbjct: 61  SFAFLVTWLTTFKLVLFSFNLGPLAFDSKLSFPLFASIAFLPTRIKHSKTAKYTQN---- 120

Query: 121 HSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLCFY 180
                EEVG+ EP PK+PLN+PAKVVI  +L++GK YY  E+V  PIAKL +NCA L FY
Sbjct: 121 ----QEEVGK-EPRPKIPLNLPAKVVIFVMLIMGKKYY--EIVD-PIAKLCMNCATLYFY 180

Query: 181 LDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYK 240
           LDM   LSN FV+S FGVEV RPF EPYLATSLQNFWGRRWN LVSETLH+ +Y+P RY+
Sbjct: 181 LDMIMCLSNVFVRSRFGVEVSRPFDEPYLATSLQNFWGRRWNRLVSETLHNAVYQPVRYE 240

Query: 241 ARRPRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 300
              PRW AV+VVFVVSGLMHEL  YY++RAIPTWE TW+FVIHG+CVAVEIELKR LGKR
Sbjct: 241 VGGPRWMAVVVVFVVSGLMHELLLYYIIRAIPTWEVTWFFVIHGLCVAVEIELKRALGKR 300

Query: 301 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMR 331
           W++H  VS PLT+AFLV T++WL FP ++R
Sbjct: 301 WRIHWAVSGPLTMAFLVATAHWLFFPPLLR 318

[Close]

BLAST of Lag0038082 vs. ExPASy TrEMBL
Match: A0A6J1IJ93 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=3661 GN=LOC111476790 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.9e-118
Identity = 226/330 (68.48%), Postives = 263/330 (79.70%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           MG E++  +KV+LI M SLLYCYFIA KLPKGK RL+S LPVFSLF+VLPLY+SS  L+S
Sbjct: 1   MGSELISLMKVILISMASLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSAFLSS 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKD 120
           + AF VTWLTTFKL+LFSF+LGPLA D  L FPLFASIAF PTR+KH+KT  Y QN    
Sbjct: 61  SFAFIVTWLTTFKLVLFSFNLGPLAFDSKLSFPLFASIAFLPTRIKHSKTAKYVQN---- 120

Query: 121 HSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLCFY 180
                EEVG+ EP PK+PLN+PAKVVI  +L++GK YY  E+V  PIAK  +NCAML FY
Sbjct: 121 ----QEEVGK-EPHPKIPLNLPAKVVIFVMLIMGKKYY--EIVD-PIAKSCMNCAMLYFY 180

Query: 181 LDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYK 240
           LDM   LSN FV+S FGVEV RPF EPYLATSLQNFWGRRWN LVSETLH+ +Y+P RY+
Sbjct: 181 LDMIMCLSNVFVRSRFGVEVSRPFNEPYLATSLQNFWGRRWNRLVSETLHNAVYQPVRYE 240

Query: 241 ARRPRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 300
              PRW AV+VVFVVSGLMHEL  YY++R IPTWE TW+FVIHG+CVAVEIELKR LGKR
Sbjct: 241 VGGPRWMAVVVVFVVSGLMHELLLYYIIRVIPTWEVTWFFVIHGLCVAVEIELKRALGKR 300

Query: 301 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMR 331
           W++H  VS PLT  FLV T++WLLFP ++R
Sbjct: 301 WRIHWAVSGPLTTVFLVATAHWLLFPPLLR 318

[Close]

BLAST of Lag0038082 vs. ExPASy TrEMBL
Match: A0A0A0K522 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G091730 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 2.4e-118
Identity = 227/335 (67.76%), Postives = 268/335 (80.00%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           M GE++  +KVLLI M SL+YCY+IASKLPKGK RLLSLLP+FSLF VLPL LS ++LTS
Sbjct: 3   MDGEVIRLMKVLLICMASLMYCYYIASKLPKGKPRLLSLLPIFSLFAVLPLDLSYVVLTS 62

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKD 120
            I  FVTWLTTFKL+ FSFDLGPLASDPPL FPLFASIAF PTR+K  KTTPY QN +KD
Sbjct: 63  GITIFVTWLTTFKLIQFSFDLGPLASDPPLSFPLFASIAFLPTRIKDIKTTPYVQNHKKD 122

Query: 121 HSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLCFY 180
            SNAP+E+ Q E PPKLPLN+PAK  I A+LV+ K++  V LV  PI KL +NC +L FY
Sbjct: 123 -SNAPQELDQ-ETPPKLPLNLPAKAFIFALLVICKNH--VHLV-HPILKLILNCGILYFY 182

Query: 181 LDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYK 240
           LDM  S+SN FV+ +FG+EV+R F EPYLATSLQNFWGRRWN LVSETLH+ IYKP RY 
Sbjct: 183 LDMIMSISNEFVRLSFGIEVRRSFDEPYLATSLQNFWGRRWNRLVSETLHNMIYKPIRYD 242

Query: 241 ARRPRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 300
              PRW AV VVFVVSGLMHEL +YY++R +PTWE TW+FVIHGV VA+EIELK  LGK+
Sbjct: 243 VGSPRWMAVFVVFVVSGLMHELLFYYIIRVVPTWEVTWFFVIHGVFVALEIELKSALGKK 302

Query: 301 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMRIDTFP 336
           WQ H  V+ PLT+AF+ VT++WL FP ++R+   P
Sbjct: 303 WQFHWAVTGPLTMAFMAVTAHWLFFPQLLRMKYSP 332

[Close]

BLAST of Lag0038082 vs. ExPASy TrEMBL
Match: A0A6J1FFF7 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita moschata OX=3662 GN=LOC111443462 PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 3.5e-117
Identity = 222/335 (66.27%), Postives = 267/335 (79.70%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           M  E++ F+KV+LI M SLLYCYFIA KLPKGK RL+S LPVFSLF+VLPLY+SS LL+S
Sbjct: 1   MSSELISFMKVILISMTSLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSALLSS 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKD 120
           + AFF+TWLTTFKL+LFSF+LGPLA D  + F LFASIAF PTR+KHNKT  Y QN    
Sbjct: 61  SFAFFITWLTTFKLVLFSFNLGPLAFDSKVSFTLFASIAFLPTRIKHNKTAKYVQN---- 120

Query: 121 HSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLCFY 180
                +++G  +P PKLPL++PAKVVI A+LV+GK +  +E++  PIA L +NCA L FY
Sbjct: 121 ----HDDLGN-DPHPKLPLSLPAKVVIFAMLVMGKRH--LEIMD-PIANLYVNCATLYFY 180

Query: 181 LDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYK 240
           LDM  SLSN FV+S FGVEV++PF EPYLATSLQNFWGRRWN  VSETLH  +Y+P RY+
Sbjct: 181 LDMTMSLSNVFVRSRFGVEVRQPFDEPYLATSLQNFWGRRWNRSVSETLHTAVYQPVRYE 240

Query: 241 ARRPRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 300
              PRW AV++VFVVSGLMHEL  YY++R IPTWE TW+FV+HG+CVAVEIELKR LGKR
Sbjct: 241 IGVPRWMAVVIVFVVSGLMHELLLYYIIRVIPTWEVTWFFVVHGLCVAVEIELKRALGKR 300

Query: 301 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMRIDTFP 336
           W++HS VSRPLTIAF V T +WL FP ++RI  FP
Sbjct: 301 WRIHSAVSRPLTIAFFVATVHWLFFPPLLRIKHFP 323

[Close]

BLAST of Lag0038082 vs. ExPASy TrEMBL
Match: A0A6J1ILW9 (probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111476789 PE=3 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 1.3e-116
Identity = 226/335 (67.46%), Postives = 262/335 (78.21%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           MG E++ F+KVLLI MVSLLYCYFIA KL KGK RL+S LPVFSLF+VLPLY+SS  L+S
Sbjct: 1   MGSELISFMKVLLISMVSLLYCYFIAQKLSKGKFRLISFLPVFSLFLVLPLYVSSAFLSS 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKD 120
            I+FFVTWLTTFKL+LFSF+LGPLA DP L   LF SIAF PTR+KHNKT  Y QN    
Sbjct: 61  NISFFVTWLTTFKLILFSFNLGPLAFDPKLSLSLFVSIAFLPTRIKHNKTAKYVQN---- 120

Query: 121 HSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLCFY 180
                +++G  EP PKLPL++PAKVVI A+LV+G  +     +  PIAKL +NCA L FY
Sbjct: 121 ----HDDLGN-EPRPKLPLSLPAKVVIFAMLVMGGRHLE---IGGPIAKLYLNCATLYFY 180

Query: 181 LDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYK 240
           LDM  SLSN FV+S FGVEV+RPF EPYLATSLQNFWGRRWN  VSETLH  +Y+P RY+
Sbjct: 181 LDMIMSLSNVFVRSRFGVEVRRPFDEPYLATSLQNFWGRRWNQSVSETLHTAVYQPVRYE 240

Query: 241 ARRPRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 300
              PRW AV+VVFVVSGLMHEL  YY++RAIPTWE TW+FV HG+CVAVEIELKR LGKR
Sbjct: 241 IGVPRWMAVVVVFVVSGLMHELLLYYIIRAIPTWEITWFFVAHGLCVAVEIELKRALGKR 300

Query: 301 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMRIDTFP 336
           W++H  VSRPLTIAFLV T +WL FP ++RI   P
Sbjct: 301 WRIHWAVSRPLTIAFLVATIHWLFFPPLLRIKHSP 323

[Close]

BLAST of Lag0038082 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 199.9 bits (507), Expect = 3.3e-51
Identity = 131/339 (38.64%), Postives = 189/339 (55.75%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           M  E+  FIK+ +  ++S+ YCY++++ +  G  RLLS+LPV +LF+V PL+ S +  + 
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPL--FASIAFFPTRVKHNKTTPYAQNLR 120
            +AFF++WL  FKL+LFSFD GPL+   PLP  L  F  I  FP + + N   P  QN  
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIKPQQN---PNIQNY- 120

Query: 121 KDHSNAPEEVGQLEPPPKLPLNMPA-KVVILAILVLGKSYYMVELVSRPIAKLSINCAML 180
                            K+P+ + A KVVI  +L+     Y  +    P   L  N   +
Sbjct: 121 -----------------KIPIWLFAIKVVIFVVLL---QMYEYKQYLSPALLLVFNSLHI 180

Query: 181 CFYLDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPT 240
              L++   L  A V  T G +++    EPYLATSLQ+FWGRRWNL+V   L   +Y P 
Sbjct: 181 FLELEIVFMLVKALVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPA 240

Query: 241 RYKARR------PRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEI 300
           R  A R        +  V   F+VSG +HE+ ++YL R +PT E TW+F++HGVC   E+
Sbjct: 241 RRMACRKVNSDQAMFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEV 300

Query: 301 ELKRTLGKR--WQLHSIVSRPLTIAFLVVTSYWLLFPLM 329
            +K++   R  W++   VSR LT+ F+VVTS W  FPL+
Sbjct: 301 AVKKSTFVRRWWRVSPTVSRLLTVGFVVVTSGWFFFPLI 312

[Close]

BLAST of Lag0038082 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 196.4 bits (498), Expect = 3.7e-50
Identity = 132/340 (38.82%), Postives = 189/340 (55.59%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           M  E+   IKV +  ++S+ YCY+I  ++  G  R LS+ PV +LF+VLPL+ SS+ L+ 
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLP--FPLFASIAFFPTRVKHNKTTPYAQNLR 120
             AFF+TWL  FKL+LFSFD GPL    P+P  FP F     FP +V+ N   P +QN  
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLI---PIPTNFPRFFCFTCFPIKVQQN---PKSQN-- 120

Query: 121 KDHSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLC 180
                           PKL   +  K+ I A+L+     Y       P   L +    L 
Sbjct: 121 --------------HLPKLVFAI--KLAIFAVLL---HLYSYRQNLSPTILLGLYFVHLY 180

Query: 181 FYLDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTR 240
             +++  +     V  + G +++    +PYLATSLQ+FWGRRWNL+V   L   +Y P R
Sbjct: 181 LEIEIILTFVKVVVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMR 240

Query: 241 YKARRPR---WA---AVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIE 300
             + R     WA    ++  F+VSGL+HEL ++YL+R +PT E T +FV+HGVC AVE+ 
Sbjct: 241 RVSERKMSSGWALFPGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELA 300

Query: 301 LKR--TLGKRWQLHSIVSRPLTIAFLVVTSYWLLFPLMMR 331
           +K+  T+ +RW+L   VSR LT+ F+ VT  WL  P + R
Sbjct: 301 VKKKTTVAQRWRLSPGVSRVLTVGFVFVTGGWLFTPQLKR 313

[Close]

BLAST of Lag0038082 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 195.3 bits (495), Expect = 8.2e-50
Identity = 134/330 (40.61%), Postives = 183/330 (55.45%), Query Frame = 0


Query: 9   IKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTSAIAFFVTW 68
           I+V +  ++SL YCY+I+SKL KG LRLLS+LPV  LF+VLPL+LS +   +    F++W
Sbjct: 9   IEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLSW 68

Query: 69  LTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKDHSNAPEEV 128
           L  FKLLLF+FD GPL    PLP  L   I F    +K          +R+D S  P  +
Sbjct: 69  LANFKLLLFAFDEGPLF---PLPPKLSRFICFACLPIK----------IRQDPS--PNAI 128

Query: 129 GQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVS--RPIAKLSINCAMLCFYLDMATS 188
             L P P     MP  V+ + ILVLG   ++ E          L++ C  +   +++   
Sbjct: 129 PNLHPKP-----MPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLV 188

Query: 189 LSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYKARR--- 248
              A V +  G  ++  F EPYLATSLQ+FW RRWNL+VS  L  T++ P +   +R   
Sbjct: 189 FVGAVVSTLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILS 248

Query: 249 ---PRWAAVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 308
                +A VM  F VSGLMHEL Y+Y++R  PTWE T +FV+HG   A EI +KRT   R
Sbjct: 249 PDGAMFAGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQWLR 308

Query: 309 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMR 331
              H  VS  + + F+ VT  WL    ++R
Sbjct: 309 -PPHRAVSGLVVLTFVSVTGVWLFLAQVLR 317

[Close]

BLAST of Lag0038082 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 193.7 bits (491), Expect = 2.4e-49
Identity = 119/340 (35.00%), Postives = 194/340 (57.06%), Query Frame = 0


Query: 8   FIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTSAIAFFVT 67
           FIK   + ++SL Y +FIA  +PKG  RL+   PVF +F ++P  + S+ L    AFF+ 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLTTFKLLLFSFDLGPLASD-PPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKDHSNAPE 127
           WL  FKLLLF+   GPL+S+  PL  P+F +++  P +++       +    K HS+   
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQ------LSPKPTKTHSHEGS 123

Query: 128 EVGQLEPPPKLPLNMPAKVVILAILVLGKSY-YMVELVSRPIAKLSINCAMLCFYLDMAT 187
             G        PL    K V + +++  K+Y Y  +L  + +  L++    + F L++  
Sbjct: 124 TEG--------PLIYTIKAVFVVLII--KAYEYSTKLPEKVV--LTLYAIHIYFALEIIL 183

Query: 188 SLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPT--RYKARR 247
           + + A V++   +E++  F +PYLATSLQ+FWGRRWNL+V+  L  T+Y+P+   +    
Sbjct: 184 AATAAAVRAMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLG 243

Query: 248 PRWAAVMV---VFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELKRTLGKR 307
           P ++ ++     FVVSG+MHEL ++Y+ R  P W+  W+F+I+G C  VEI +K+T+  R
Sbjct: 244 PNYSQILAAFGTFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGR 303

Query: 308 WQLHSIVSRPLTIAFLVVTSYWLLFPLMMRIDTFPHMEDE 341
           W+    +S+ LT+ F++VT+ WL  P   R +      DE
Sbjct: 304 WRFPKAISQVLTLTFVMVTALWLFLPEFNRCNIVEKALDE 325

[Close]

BLAST of Lag0038082 vs. TAIR 10
Match: AT1G34490.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 191.4 bits (485), Expect = 1.2e-48
Identity = 135/346 (39.02%), Postives = 193/346 (55.78%), Query Frame = 0


Query: 1   MGGEILGFIKVLLIFMVSLLYCYFIASKLPKGKLRLLSLLPVFSLFVVLPLYLSSILLTS 60
           M  E+  F+KV    ++S+ YCY+I SK+ +G  RLLS+LPV  LF+VLPL+    + +S
Sbjct: 1   MEEELKSFVKVWGSAIISVSYCYYIPSKIKRGVHRLLSVLPVCVLFLVLPLFFVFTIFSS 60

Query: 61  AIAFFVTWLTTFKLLLFSFDLGPLASDPPLPFPLFASIAFFPTRVKHNKTTPYAQNLRKD 120
             AF ++ L  FKL+LF+FD GPL    PLP  LF  I F    +K  +T P +QN    
Sbjct: 61  TTAFCLSILANFKLILFAFDKGPLL---PLPTNLFRFICFTCLPIK-LQTKPNSQN---- 120

Query: 121 HSNAPEEVGQLEPPPKLPLNMPAKVVILAILVLGKSYYMVELVSRPIAKLSINCAMLCFY 180
             + P+ V            +P+KV I  +L+  +SY   +++  PI  L +    L   
Sbjct: 121 --HLPKWV------------LPSKVAIFVLLLNIRSY---KILLPPILLLGLYPLHLYIV 180

Query: 181 LDMATSLSNAFVQSTFGVEVKRPFIEPYLATSLQNFWGRRWNLLVSETLHDTIYKPTRYK 240
           LD+  ++ NA +      +++  F EPYLATSLQ+FWG RWNL+VS      +Y P R  
Sbjct: 181 LDVLLTIVNALLTIILRCDLEPHFNEPYLATSLQDFWGHRWNLMVSAIYRPGVYSPVRSV 240

Query: 241 AR---RPRWA---AVMVVFVVSGLMHELWYYYLVRAIPTWEATWYFVIHGVCVAVEIELK 300
            +   R  WA     M  F VSGL+HEL Y+Y+ R  PT E TW+FV+HGVC A+EI +K
Sbjct: 241 CQHQMRSDWARFMGCMTTFFVSGLIHELVYFYINREKPTLEVTWFFVLHGVCTAMEIAVK 300

Query: 301 RTLGKRWQLHSIVSRPLTIAFLVVTSYWLLFPLMMRIDTFPHMEDE 341
           R +  +W L  ++ R +T+ FLVVT   L F  + R +      +E
Sbjct: 301 RKM--QWSLSPMLLRLITVGFLVVTGDLLFFGQIERSNMLERRANE 319

The following BLAST results are available for this feature:

BLAST of Lag0038082 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899290.1	3.6e-124	71.34	probable long-chain-alcohol O-fatty-acyltransferase 5 [Benincasa hispida]	[more]
XP_022936880.1	1.6e-119	69.09	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata]	[more]
XP_023536076.1	3.5e-119	69.09	probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pep...	[more]
XP_022976365.1	3.9e-118	68.48	probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima]	[more]
XP_004151023.1	5.0e-118	67.76	probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis sativus] >KGN4400...	[more]

BLAST of Lag0038082 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJ74	4.7e-50	38.64	Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX...	[more]
Q9FJ76	5.2e-49	38.82	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9FJ72	1.1e-48	40.61	Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX...	[more]
Q9SV07	3.3e-48	35.00	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9FJ75	1.7e-47	35.98	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]

BLAST of Lag0038082 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FEY7	7.6e-120	69.09	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1IJ93	1.9e-118	68.48	probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=366...	[more]
A0A0A0K522	2.4e-118	67.76	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G091730 PE=...	[more]
A0A6J1FFF7	3.5e-117	66.27	probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita moschata OX=3...	[more]
A0A6J1ILW9	1.3e-116	67.46	probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Cucurbita maxima OX=366...	[more]

BLAST of Lag0038082 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55360.1	3.3e-51	38.64	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55340.1	3.7e-50	38.82	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	8.2e-50	40.61	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT3G51970.1	2.4e-49	35.00	acyl-CoA sterol acyl transferase 1	[more]
AT1G34490.1	1.2e-48	39.02	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 204..281 e-value: 2.9E-15 score: 56.3
None	No IPR available	PANTHER	PTHR31595:SF8	(MEMBRANE BOUND O-ACYL TRANSFERASE) FAMILY PROTEIN, PUTATIVE-RELATED	coord: 1..334
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 1..334

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0038082.1	Lag0038082.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity