Lag0038028 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0038028 vs. NCBI nr
Match: XP_038899676.1 (taxadiene 5-alpha hydroxylase-like [Benincasa hispida])

HSP 1 Score: 794.7 bits (2051), Expect = 4.7e-226
Identity = 405/486 (83.33%), Postives = 436/486 (89.71%), Query Frame = 0

Query: 3   IITITLLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSAE 62
           +ITI LLL+T   FL    R ++ SS+A  AKNLPPGPLGVPFIGQSLSLLRAMR NSAE
Sbjct: 1   MITIFLLLITIFLFL----RFRTTSSSAATAKNLPPGPLGVPFIGQSLSLLRAMRTNSAE 60

Query: 63  EWLQQRVAKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGERN 122
           +WLQ+RVAKYG VSKM LFGKPTVFV GAAAN+AVVF GEE  VSN Q ESL+ ILGERN
Sbjct: 61  QWLQKRVAKYGPVSKMTLFGKPTVFVHGAAANKAVVFLGEEGTVSNRQIESLKWILGERN 120

Query: 123 LTELSGEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTLT 182
           LTELSGEDHKRVRGA+VWFLRPQ L+ +V KMDGEVRKHLNMYWH   +V V PLMKTLT
Sbjct: 121 LTELSGEDHKRVRGALVWFLRPQTLKSYVGKMDGEVRKHLNMYWHGKKEVTVAPLMKTLT 180

Query: 183 FDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQL 242
           FDIICSLLFGIEEGPTRKSII+CFKTMVDGIWS+PINLPFTRYNHSLKASAK QQ+LKQL
Sbjct: 181 FDIICSLLFGIEEGPTRKSIIQCFKTMVDGIWSVPINLPFTRYNHSLKASAKAQQILKQL 240

Query: 243 LKERRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHDT 302
           LK++ QKMEEEEEE++E +   KDLITYLLS+KNKDKEQALSEEEIVHN I+LMIAGHDT
Sbjct: 241 LKDKAQKMEEEEEEEKEDD---KDLITYLLSIKNKDKEQALSEEEIVHNTILLMIAGHDT 300

Query: 303 TSILLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLRL 362
           TSILLTLMLRV ATNPTVYA VLQEHEEIARSKE GE L WEDVSKMKYTWRVA+ETLR+
Sbjct: 301 TSILLTLMLRVLATNPTVYAAVLQEHEEIARSKESGEALTWEDVSKMKYTWRVAMETLRI 360

Query: 363 FPPVFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDK-QTSI 422
           +PPVFGGFRVALKDI+ GGYTIPKGWQIFWAAPMTHLDETIF +PQKFEPSRFD+ QT I
Sbjct: 361 YPPVFGGFRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGEPQKFEPSRFDQNQTPI 420

Query: 423 PPFSYIAFGGGPRICPGNEFAKLETLVTIHYLITQFTWKLSSSQDFLTRDPTLTPNKGLP 482
           PPF +IAFGGGPRICPG+EFAKLETLVTIHYLITQFTWKLS SQDFLTRDPTL PNKGLP
Sbjct: 421 PPFCFIAFGGGPRICPGSEFAKLETLVTIHYLITQFTWKLSCSQDFLTRDPTLMPNKGLP 479

Query: 483 IQIFPK 488
           IQI PK
Sbjct: 481 IQISPK 479

BLAST of Lag0038028 vs. NCBI nr
Match: XP_022936436.1 (taxadiene 5-alpha hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 785.4 bits (2027), Expect = 2.8e-223
Identity = 406/487 (83.37%), Postives = 438/487 (89.94%), Query Frame = 0

Query: 3   IITITLLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSAE 62
           +I I LLL+T   +L   R R +  STA AAKNLPPG LGVP+IGQSLSLLRAMRANSAE
Sbjct: 1   MIAIILLLITIFVYL---RLRTTFFSTAVAAKNLPPGRLGVPYIGQSLSLLRAMRANSAE 60

Query: 63  EWLQQRVAKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGERN 122
           +WLQQRVAKYG +SKM LFGKPTVFV G AAN+AVVFSGEE AVSN QTESLRRILGERN
Sbjct: 61  QWLQQRVAKYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGERN 120

Query: 123 LTELSGEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTLT 182
           LTELSGEDHKRVRGA+VWFLRPQ+L+ FV KMDGEVRKHL ++WH N +VIV PLMKTLT
Sbjct: 121 LTELSGEDHKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLKLHWHGNKEVIVAPLMKTLT 180

Query: 183 FDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQL 242
           FDIICSLLFGIEEGPTRKSIIECFKTMVDGIWS+PINLPFTRY+HSLKASAKVQQ+LKQL
Sbjct: 181 FDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQL 240

Query: 243 LKERRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHDT 302
           LK++ QKME EEEEKEE     K+LIT LLS+KNKDKEQALSEEEIVHNII+L+IAGHDT
Sbjct: 241 LKDKAQKMEGEEEEKEE----DKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDT 300

Query: 303 TSILLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLRL 362
           TSIL+TLMLRV ATNPTVYA VLQEHE+I RSKECGE L WEDVSKMKYTWRVALETLRL
Sbjct: 301 TSILITLMLRVMATNPTVYAAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRL 360

Query: 363 FPPVFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDK-QTSI 422
           FPPVFGGFRVALKDI+ GGYTIPKGWQIFWAA MTHLDE IF DPQKFEPSRFD+ QTSI
Sbjct: 361 FPPVFGGFRVALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSI 420

Query: 423 -PPFSYIAFGGGPRICPGNEFAKLETLVTIHYLITQFTWKLSSSQDFLTRDPTLTPNKGL 482
            PP S+IAFGGGPRICPG EFAKLETLVTIHY+ITQFTWKL SSQDFLTRDP+L PNKGL
Sbjct: 421 PPPLSFIAFGGGPRICPGYEFAKLETLVTIHYIITQFTWKL-SSQDFLTRDPSLMPNKGL 479

Query: 483 PIQIFPK 488
           PIQ++PK
Sbjct: 481 PIQLYPK 479

BLAST of Lag0038028 vs. NCBI nr
Match: XP_022976494.1 (taxadiene 5-alpha hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 783.5 bits (2022), Expect = 1.1e-222
Identity = 407/488 (83.40%), Postives = 438/488 (89.75%), Query Frame = 0

Query: 2   MIITITLLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSA 61
           MI TI LL+    T  L  R R +  S+A  AKNLPPGPLGVPFIGQSLSLLRAMRANSA
Sbjct: 1   MIATILLLI----TIFLYLRLRATFFSSA-EAKNLPPGPLGVPFIGQSLSLLRAMRANSA 60

Query: 62  EEWLQQRVAKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGER 121
           E+WLQQRVAKYG +SKM LFGKPTVFV G AAN+AVVFSGEE AVSN QTESLRRILGER
Sbjct: 61  EQWLQQRVAKYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGER 120

Query: 122 NLTELSGEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTL 181
           NLTELSGEDHKRVRGA+VWFLRPQ+L+ FV KMDGEVRKHLN++WH N +VIV PLMKTL
Sbjct: 121 NLTELSGEDHKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTL 180

Query: 182 TFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQ 241
           TFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWS+PINLPFTRY+HSL ASAKVQQ+LKQ
Sbjct: 181 TFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLNASAKVQQILKQ 240

Query: 242 LLKERRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHD 301
           LLK++ QKMEEEEEEKEE     K+LIT LLS+KNKDKEQALSEEEIVHNII+L+IAGHD
Sbjct: 241 LLKDKAQKMEEEEEEKEE----DKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHD 300

Query: 302 TTSILLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLR 361
           TTSIL+TLMLRV ATNPTVYA VLQEHE+I RSKECGE L WEDVSKMKYTWRVALETLR
Sbjct: 301 TTSILITLMLRVMATNPTVYAAVLQEHEDIHRSKECGEALTWEDVSKMKYTWRVALETLR 360

Query: 362 LFPPVFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDK-QTS 421
           LFPPVFGGFRVALKDI+ GGYTIPKGWQIFWAA MTHLDE IF DPQKF+PSRFD+ QTS
Sbjct: 361 LFPPVFGGFRVALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFTDPQKFKPSRFDQNQTS 420

Query: 422 I-PPFSYIAFGGGPRICPGNEFAKLETLVTIHYLITQFTWKLSSSQDFLTRDPTLTPNKG 481
           I PP S+IAFGGGPRICPG EFAKLETLVTIHY+ITQFTWKL SSQDFLTRDP+L PNKG
Sbjct: 421 IPPPLSFIAFGGGPRICPGYEFAKLETLVTIHYIITQFTWKL-SSQDFLTRDPSLMPNKG 478

Query: 482 LPIQIFPK 488
           LPIQ++PK
Sbjct: 481 LPIQLYPK 478

BLAST of Lag0038028 vs. NCBI nr
Match: XP_004152028.1 (taxadiene 5-alpha hydroxylase [Cucumis sativus] >KGN57973.1 hypothetical protein Csa_010468 [Cucumis sativus])

HSP 1 Score: 777.3 bits (2006), Expect = 7.7e-221
Identity = 398/483 (82.40%), Postives = 427/483 (88.41%), Query Frame = 0

Query: 6   ITLLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSAEEWL 65
           IT+++L   T  L  R R  LSS  +AAKNLPPG LGVPFIGQSLSLL AMR N+AE+WL
Sbjct: 2   ITVIILLVLTIFLFHRFRTRLSS--SAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWL 61

Query: 66  QQRVAKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGERNLTE 125
           Q+RV KYG VSKM LFGKPTVF+ GAAAN+AVVFSGEE  VSN Q ESL+ ILG+RNLTE
Sbjct: 62  QKRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTE 121

Query: 126 LSGEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTLTFDI 185
           LSGEDHKRVRGA+VWFLRPQ LR +V KMDGEVR+HLNMYWH NN+V V PLMKTLTFDI
Sbjct: 122 LSGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDI 181

Query: 186 ICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQLLKE 245
           ICSLLFGIEEG TRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAK QQ+LKQLLK+
Sbjct: 182 ICSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKD 241

Query: 246 RRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHDTTSI 305
           + + MEEE+E   E E+  KDLI+YLL +KNKDKEQALSEEEIVHNII+LMIAGHDTTSI
Sbjct: 242 KAKVMEEEKE--SEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSI 301

Query: 306 LLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLRLFPP 365
           LLTLMLRV ATNPTVYA VLQEHEEI RSKE GE L WEDVSKMKYTWRVA+ETLRL+PP
Sbjct: 302 LLTLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPP 361

Query: 366 VFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDK-QTSIPPF 425
           VFGGFRVALKDI+ G YTIPKGWQIFWAAPMTHLDETIF DPQKFEPSRFD+ QT IPPF
Sbjct: 362 VFGGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPF 421

Query: 426 SYIAFGGGPRICPGNEFAKLETLVTIHYLITQFTWKLSSSQDFLTRDPTLTPNKGLPIQI 485
           S+IAFGGGPRICPG EFAKLETLVTIHYLITQFTW LS SQDFLTRDPTL PNKGLPIQI
Sbjct: 422 SFIAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQI 480

Query: 486 FPK 488
           FPK
Sbjct: 482 FPK 480

BLAST of Lag0038028 vs. NCBI nr
Match: XP_023535435.1 (taxadiene 5-alpha hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 775.4 bits (2001), Expect = 2.9e-220
Identity = 399/484 (82.44%), Postives = 434/484 (89.67%), Query Frame = 0

Query: 6   ITLLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSAEEWL 65
           I ++LL    FL  R R    S+   AAKNLPPG LGVP+IGQSLSLLRAMRANSAE+WL
Sbjct: 2   IAIILLLITIFLYLRLRTTFFSA---AAKNLPPGRLGVPYIGQSLSLLRAMRANSAEQWL 61

Query: 66  QQRVAKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGERNLTE 125
           QQRVAKYG +SKM LFGKPTVFV G AAN+AVVF GEE AVSN QTESLRRILGERNLTE
Sbjct: 62  QQRVAKYGPISKMTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTE 121

Query: 126 LSGEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTLTFDI 185
           LSGEDHKRVRGA+VWFLRPQ+L+ FV KMDGEVRKHLN++WH N +VIV PLMKTLTFDI
Sbjct: 122 LSGEDHKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDI 181

Query: 186 ICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQLLKE 245
           ICSLLFGIEEGPTRKSIIECFKTMVDGIWS+PINLPFTRY+HSLKASAKVQQ+LKQLLK+
Sbjct: 182 ICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKD 241

Query: 246 RRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHDTTSI 305
           + QKMEEEEE++E+     K+LIT LLS+KNKDKEQALSEEEIVHNII+L+IAGHDTTSI
Sbjct: 242 KAQKMEEEEEKEED-----KNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSI 301

Query: 306 LLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLRLFPP 365
           L+TLMLRV ATNPTVYA VLQEHE+I RSKECGE L WEDVSKMKYTWRVALETLRLFPP
Sbjct: 302 LITLMLRVMATNPTVYAAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPP 361

Query: 366 VFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDK-QTSI-PP 425
           VFGGFR+ALKDI+ GGYTIPKGWQIFWAA MTHLDE IF DPQKFEPSRFD+ QTSI PP
Sbjct: 362 VFGGFRMALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPP 421

Query: 426 FSYIAFGGGPRICPGNEFAKLETLVTIHYLITQFTWKLSSSQDFLTRDPTLTPNKGLPIQ 485
            S+IAFGGGPRICPG EFAKLETLVTIHY+ITQFTWKL SSQDFLTRDP+L PNKGLPIQ
Sbjct: 422 LSFIAFGGGPRICPGYEFAKLETLVTIHYIITQFTWKL-SSQDFLTRDPSLMPNKGLPIQ 476

Query: 486 IFPK 488
           ++PK
Sbjct: 482 LYPK 476

BLAST of Lag0038028 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 4.5e-91
Identity = 181/456 (39.69%), Postives = 270/456 (59.21%), Query Frame = 0

Query: 32  AAKNLPPGPLGVPFIGQSLSLLRAMRANSAEEWLQQRVAKYGAVSKMGLFGKPTVFVSGA 91
           A + +PPG  G P IG++L  L   R  +  ++   R  KYG V    L G PTV     
Sbjct: 47  AWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFDSRTQKYGNVFTTSLVGHPTVVFCSP 106

Query: 92  AANRAVVFSGEEAAVSNAQTESLRRILGERNLTELSGEDHKRVRGAVVWFLRPQNLREFV 151
             NR  +FS E   V N+   S+  +     +T + G+D KR+R  ++ FLRP+ LREFV
Sbjct: 107 EGNR-FLFSNENKLVVNSWPSSVGNLFRSSLITTV-GDDAKRLRRILMTFLRPEALREFV 166

Query: 152 RKMDGEVRKHLNMYWHPNNQVIVNPLMKTLTFDIICSLLFGIEEGPTRKSIIECFKTMVD 211
            ++D   ++HL  +W   ++V+  PL+K  TF + C L   I        +   F   V 
Sbjct: 167 GRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLACDLFASINTKDDLDRLWLHFMVFVK 226

Query: 212 GIWSIPINLPFTRYNHSLKASAKVQQMLKQLLKERRQKMEEEEEEKEEGEKKHKDLITYL 271
           G+  IPI+LP TRYN +  A+  ++Q L  ++ ER+  +E      E+      DL+++L
Sbjct: 227 GVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINERKIALEAGNASPEQ------DLLSFL 286

Query: 272 LSLKNKDKE-QALSEEEIVHNIIILMIAGHDTTSILLTLMLRVFATNPTVYATVLQEHEE 331
           LS  N D++ ++L++ EI  NI++L+ AGHDT+S  LT++L+  A NP  Y  VL+E   
Sbjct: 287 LS--NVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLREQLN 346

Query: 332 IARSKECGEPLNWEDVSKMKYTWRVALETLRLFPPVFGGFRVALKDIEFGGYTIPKGWQI 391
           IA SKE G+ L WED+ +MKY+WRVA E LRLFP V G FR A+K+  + G+TIPKGW++
Sbjct: 347 IAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSFRKAIKEFIYDGFTIPKGWKL 406

Query: 392 FWAAPMTHLDETIFADPQKFEPSRFDKQTSIPPFSYIAFGGGPRICPGNEFAKLETLVTI 451
            W    TH     F++P+KF+PSRF+ +   PP++++ FGGGPR+CPGNEFA++E L+ +
Sbjct: 407 HWTVNSTHQKSEYFSNPEKFDPSRFEGE-GPPPYTFVPFGGGPRMCPGNEFARMEILIFL 466

Query: 452 HYLITQFTWKLSSSQDFLTRDPTLTPNKGLPIQIFP 487
           H ++  F W L +  + +  DP   P  GLPI++ P
Sbjct: 467 HNIVKNFNWNLVNPLEKVIVDPMPAPVNGLPIKLVP 491

BLAST of Lag0038028 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 6.6e-90
Identity = 180/456 (39.47%), Postives = 268/456 (58.77%), Query Frame = 0

Query: 32  AAKNLPPGPLGVPFIGQSLSLLRAMRANSAEEWLQQRVAKYGAVSKMGLFGKPTVFVSGA 91
           A + +PPG  G P IG++L  L   R  +  ++   R  KYG V    L G PTV     
Sbjct: 47  AWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFDSRTQKYGNVFTTSLVGHPTVVFCSP 106

Query: 92  AANRAVVFSGEEAAVSNAQTESLRRILGERNLTELSGEDHKRVRGAVVWFLRPQNLREFV 151
             NR  +FS E   V N+   S+  +     +T + G+D KR+R  ++ FLRP+ LREFV
Sbjct: 107 EGNR-FLFSNENKLVVNSWPSSVGNLFRSSLITTV-GDDAKRLRRILMTFLRPEALREFV 166

Query: 152 RKMDGEVRKHLNMYWHPNNQVIVNPLMKTLTFDIICSLLFGIEEGPTRKSIIECFKTMVD 211
            ++D   ++HL  +W   ++V   PL+K  TF + C L   I        +   F   V 
Sbjct: 167 GRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLACDLFASINNKDDLGRLWLHFMVFVK 226

Query: 212 GIWSIPINLPFTRYNHSLKASAKVQQMLKQLLKERRQKMEEEEEEKEEGEKKHKDLITYL 271
           G+  IPI+LP TRYN +  A+  ++Q L  ++ ER+  +E      E+      DL+++L
Sbjct: 227 GVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINERKIGLEAGNASPEQ------DLLSFL 286

Query: 272 LSLKNKDKE-QALSEEEIVHNIIILMIAGHDTTSILLTLMLRVFATNPTVYATVLQEHEE 331
           LS  N D++ ++L++ EI  NI++L+ AGHDT+S  LT++L+  A NP  Y  VL+E  +
Sbjct: 287 LS--NVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLREQLD 346

Query: 332 IARSKECGEPLNWEDVSKMKYTWRVALETLRLFPPVFGGFRVALKDIEFGGYTIPKGWQI 391
           IA SKE G+ L WED+ +MKY+WRVA E LRLFP   G FR A+K+  + G+TIPKGW++
Sbjct: 347 IAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSFRKAIKEFIYDGFTIPKGWKM 406

Query: 392 FWAAPMTHLDETIFADPQKFEPSRFDKQTSIPPFSYIAFGGGPRICPGNEFAKLETLVTI 451
           +W    TH     F++P+ F+PSRF+ +   PP++++ FGGGPR+CPGNEFA+LE LV +
Sbjct: 407 YWTVNSTHRKSEYFSNPETFDPSRFEGE-GPPPYTFVPFGGGPRMCPGNEFARLEILVFL 466

Query: 452 HYLITQFTWKLSSSQDFLTRDPTLTPNKGLPIQIFP 487
           H ++    W L +  + +  DP   P  GLPI++ P
Sbjct: 467 HNIVKNCKWNLVNPGEKVIVDPMPAPVNGLPIKLVP 491

BLAST of Lag0038028 vs. ExPASy Swiss-Prot
Match: Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)

HSP 1 Score: 325.9 bits (834), Expect = 8.0e-88
Identity = 182/488 (37.30%), Postives = 294/488 (60.25%), Query Frame = 0

Query: 3   IITITLLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSAE 62
           I  I LLLL     L R +R  SL         LPPG LG+PFIG+S   LRA+R+NS E
Sbjct: 32  IAGILLLLL-----LFRSKRHSSL--------KLPPGKLGIPFIGESFIFLRALRSNSLE 91

Query: 63  EWLQQRVAKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGERN 122
           ++  +RV K+G V K  L G PTV + G A NR ++ S EE  V  +      +++GE +
Sbjct: 92  QFFDERVKKFGLVFKTSLIGHPTVVLCGPAGNR-LILSNEEKLVQMSWPAQFMKLMGENS 151

Query: 123 LTELSGEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTLT 182
           +    GEDH  +R A+  F  P  L+ ++ KM+ E++ H+N  W   ++V V PL++ L 
Sbjct: 152 VATRRGEDHIVMRSALAGFFGPGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELV 211

Query: 183 FDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQL 242
           F+I   L F I +   +  + +  +T++ G +++PI+LP   ++ +L+  AK+ +++  L
Sbjct: 212 FNISAILFFNIYDKQEQDRLHKLLETILVGSFALPIDLPGFGFHRALQGRAKLNKIMLSL 271

Query: 243 LKERRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHDT 302
           +K+R++ ++       +      DL++ LL+ ++ DK   L+ +EI+ N   L+ A +DT
Sbjct: 272 IKKRKEDLQSGSATATQ------DLLSVLLTFRD-DKGTPLTNDEILDNFSSLLHASYDT 331

Query: 303 TSILLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLRL 362
           T+  + L+ ++ ++NP  Y  V+QE  EI  +KE GE + W+D+  MKYTW+VA ETLR+
Sbjct: 332 TTSPMALIFKLLSSNPECYQKVVQEQLEILSNKEEGEEITWKDLKAMKYTWQVAQETLRM 391

Query: 363 FPPVFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDKQ-TSI 422
           FPPVFG FR A+ DI++ GYTIPKGW++ W    TH  +  F +P+KF PSRFD++   +
Sbjct: 392 FPPVFGTFRKAITDIQYDGYTIPKGWKLLWTTYSTHPKDLYFNEPEKFMPSRFDQEGKHV 451

Query: 423 PPFSYIAFGGGPRICPGNEFAKLETLVTIHYLITQF-TWKLSSSQDFLTRDP-TLTPNKG 482
            P++++ FGGG R C G EF+K+E L+ +H+ +  F ++      + ++ DP    P+KG
Sbjct: 452 APYTFLPFGGGQRSCVGWEFSKMEILLFVHHFVKTFSSYTPVDPDEKISGDPLPPLPSKG 498

Query: 483 LPIQIFPK 488
             I++FP+
Sbjct: 512 FSIKLFPR 498

BLAST of Lag0038028 vs. ExPASy Swiss-Prot
Match: Q84KI1 (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 9.8e-86
Identity = 171/476 (35.92%), Postives = 279/476 (58.61%), Query Frame = 0

Query: 18  LRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSAEEWLQQRVAKYGAVSK 77
           LR +RR S+         LPPG LG PFIG+SL  L+A+R+N+ E++L +RV  +G V K
Sbjct: 40  LRSKRRSSV--------GLPPGKLGYPFIGESLLFLKALRSNTVEQFLDERVKNFGNVFK 99

Query: 78  MGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGERNLTELSGEDHKRVRGA 137
             L G PTV + G A NR ++ + EE  V  +  +S  +++GE+++T   GE H  +R A
Sbjct: 100 TSLIGHPTVVLCGPAGNR-LILANEEKLVQMSWPKSSMKLMGEKSITAKRGEGHMIIRSA 159

Query: 138 VVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTLTFDIICSLLFGIEEGP 197
           +  F  P  L++++ +M   +  H+N  W  N+QV V  L+  L FDI   L F I E  
Sbjct: 160 LQGFFSPGALQKYIGQMSKTIENHINEKWKGNDQVSVVALVGDLVFDISACLFFNINEKH 219

Query: 198 TRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQLLKERRQKMEEEEEEK 257
            R+ + E  + +  G+ ++P++LP   Y+ +L+A +K+  +L  L+++R+  +       
Sbjct: 220 ERERLFELLEIIAVGVLAVPVDLPGFAYHRALQARSKLNAILSGLIEKRKMDLSSGL--- 279

Query: 258 EEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHDTTSILLTLMLRVFATN 317
                 ++DL++  L+ K+ D+    S+EEI+ N   L+   +DTT   +  + ++ ++N
Sbjct: 280 ---ATSNQDLLSVFLTFKD-DRGNPCSDEEILDNFSGLLHGSYDTTVSAMACVFKLLSSN 339

Query: 318 PTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLRLFPPVFGGFRVALKDI 377
           P  Y  V+QE   I  +K  G+ + W+DV  MKYTW+V  ETLRL+P +FG FR A+ DI
Sbjct: 340 PECYEKVVQEQLGILSNKLEGDEITWKDVKSMKYTWQVVQETLRLYPSIFGSFRQAITDI 399

Query: 378 EFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDKQTS-IPPFSYIAFGGGPRIC 437
            + GY IPKGW++ W    TH  E  F++P+KF PSRFD++   + P++++ FGGG R C
Sbjct: 400 HYNGYIIPKGWKLLWTPYTTHPKEMYFSEPEKFLPSRFDQEGKLVAPYTFLPFGGGQRSC 459

Query: 438 PGNEFAKLETLVTIHYLITQF-TWKLSSSQDFLTRDPTL-TPNKGLPIQIFPKQTS 491
           PG EF+K+E L+++H+ +  F T+      + + RD     P+ G  +++FP+  S
Sbjct: 460 PGWEFSKMEILLSVHHFVKTFSTFTPVDPAEIIARDSLCPLPSNGFSVKLFPRSYS 499

BLAST of Lag0038028 vs. ExPASy Swiss-Prot
Match: A0A0U2U8U5 (Beta-amyrin 28-monooxygenase OS=Barbarea vulgaris OX=50459 GN=CYP716A81 PE=1 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 2.2e-85
Identity = 180/490 (36.73%), Postives = 291/490 (59.39%), Query Frame = 0

Query: 2   MIITITLLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSA 61
           M +TI  L +++    L    RK LS    + +NLPPG  G P IG+SLS L   R    
Sbjct: 1   MYLTILFLFVSSILLSLMFLLRKHLSH--FSYQNLPPGKTGFPLIGESLSFLSEGRQGRP 60

Query: 62  EEWLQQRVAKY----GAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRI 121
           E+++  RV ++      V K  LFG PT  ++GA+ N+  +F+ E   V +   +S+++I
Sbjct: 61  EKFVTDRVRRFISSSTGVFKTHLFGYPTAVMTGASGNK-FLFTNENKLVVSWWPDSVKKI 120

Query: 122 LGERNLTELSGEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPL 181
                 T    ++ K++R  ++ F++P+ LR+++  MD   ++H    W    Q+IV PL
Sbjct: 121 FPYTQSTYT--DESKKLRILLMQFMKPEALRKYIGVMDEVAQRHFETLWTNQKQLIVYPL 180

Query: 182 MKTLTFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQ 241
            K LTF + C L   +++      + + F ++V GI+S+PI+LP TR+N S+KAS  +++
Sbjct: 181 SKKLTFSVACRLFLSMDDPERVSKLEDRFNSVVTGIYSVPIDLPGTRFNRSIKASRLLRK 240

Query: 242 MLKQLLKERRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMI 301
            +  ++ +RR++++      E+      D+++++L    + K     +E++ + +I ++I
Sbjct: 241 EVCAIIGQRREELKAGRASAEQ------DVLSHMLMSVGETK-----DEDLANYLIGILI 300

Query: 302 AGHDTTSILLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVAL 361
            GHDT +I  T ++   A  P VY  VLQE +EI + K+  E L WED+ KMKY+W VA 
Sbjct: 301 GGHDTAAIATTFIINYLAEYPHVYQRVLQEQKEILKEKKEEERLKWEDIEKMKYSWNVAC 360

Query: 362 ETLRLFPPVFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDK 421
           E +RL PP+ G FR A+    F G+ IPKGW+++W+A  TH++   F +P++FEP+RF+ 
Sbjct: 361 EVMRLVPPLTGNFREAIDHFTFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFE- 420

Query: 422 QTSIPPFSYIAFGGGPRICPGNEFAKLETLVTIHYLITQFTW-KLSSSQDFLTRDPTLTP 481
            +   P+SYI FGGGPR+CPG EFA+LE LV IH L+ +F W K+  +++ +  DP   P
Sbjct: 421 GSGPKPYSYIPFGGGPRMCPGREFARLEILVIIHNLVNRFKWEKVFPNENKIVVDPLPKP 473

Query: 482 NKGLPIQIFP 487
             GLPI+IFP
Sbjct: 481 GNGLPIRIFP 473

BLAST of Lag0038028 vs. ExPASy TrEMBL
Match: A0A6J1F7G6 (taxadiene 5-alpha hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443055 PE=3 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.4e-223
Identity = 406/487 (83.37%), Postives = 438/487 (89.94%), Query Frame = 0

Query: 3   IITITLLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSAE 62
           +I I LLL+T   +L   R R +  STA AAKNLPPG LGVP+IGQSLSLLRAMRANSAE
Sbjct: 1   MIAIILLLITIFVYL---RLRTTFFSTAVAAKNLPPGRLGVPYIGQSLSLLRAMRANSAE 60

Query: 63  EWLQQRVAKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGERN 122
           +WLQQRVAKYG +SKM LFGKPTVFV G AAN+AVVFSGEE AVSN QTESLRRILGERN
Sbjct: 61  QWLQQRVAKYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGERN 120

Query: 123 LTELSGEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTLT 182
           LTELSGEDHKRVRGA+VWFLRPQ+L+ FV KMDGEVRKHL ++WH N +VIV PLMKTLT
Sbjct: 121 LTELSGEDHKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLKLHWHGNKEVIVAPLMKTLT 180

Query: 183 FDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQL 242
           FDIICSLLFGIEEGPTRKSIIECFKTMVDGIWS+PINLPFTRY+HSLKASAKVQQ+LKQL
Sbjct: 181 FDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQL 240

Query: 243 LKERRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHDT 302
           LK++ QKME EEEEKEE     K+LIT LLS+KNKDKEQALSEEEIVHNII+L+IAGHDT
Sbjct: 241 LKDKAQKMEGEEEEKEE----DKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDT 300

Query: 303 TSILLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLRL 362
           TSIL+TLMLRV ATNPTVYA VLQEHE+I RSKECGE L WEDVSKMKYTWRVALETLRL
Sbjct: 301 TSILITLMLRVMATNPTVYAAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRL 360

Query: 363 FPPVFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDK-QTSI 422
           FPPVFGGFRVALKDI+ GGYTIPKGWQIFWAA MTHLDE IF DPQKFEPSRFD+ QTSI
Sbjct: 361 FPPVFGGFRVALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSI 420

Query: 423 -PPFSYIAFGGGPRICPGNEFAKLETLVTIHYLITQFTWKLSSSQDFLTRDPTLTPNKGL 482
            PP S+IAFGGGPRICPG EFAKLETLVTIHY+ITQFTWKL SSQDFLTRDP+L PNKGL
Sbjct: 421 PPPLSFIAFGGGPRICPGYEFAKLETLVTIHYIITQFTWKL-SSQDFLTRDPSLMPNKGL 479

Query: 483 PIQIFPK 488
           PIQ++PK
Sbjct: 481 PIQLYPK 479

BLAST of Lag0038028 vs. ExPASy TrEMBL
Match: A0A6J1INM7 (taxadiene 5-alpha hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111476875 PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 5.2e-223
Identity = 407/488 (83.40%), Postives = 438/488 (89.75%), Query Frame = 0

Query: 2   MIITITLLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSA 61
           MI TI LL+    T  L  R R +  S+A  AKNLPPGPLGVPFIGQSLSLLRAMRANSA
Sbjct: 1   MIATILLLI----TIFLYLRLRATFFSSA-EAKNLPPGPLGVPFIGQSLSLLRAMRANSA 60

Query: 62  EEWLQQRVAKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGER 121
           E+WLQQRVAKYG +SKM LFGKPTVFV G AAN+AVVFSGEE AVSN QTESLRRILGER
Sbjct: 61  EQWLQQRVAKYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGER 120

Query: 122 NLTELSGEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTL 181
           NLTELSGEDHKRVRGA+VWFLRPQ+L+ FV KMDGEVRKHLN++WH N +VIV PLMKTL
Sbjct: 121 NLTELSGEDHKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTL 180

Query: 182 TFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQ 241
           TFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWS+PINLPFTRY+HSL ASAKVQQ+LKQ
Sbjct: 181 TFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLNASAKVQQILKQ 240

Query: 242 LLKERRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHD 301
           LLK++ QKMEEEEEEKEE     K+LIT LLS+KNKDKEQALSEEEIVHNII+L+IAGHD
Sbjct: 241 LLKDKAQKMEEEEEEKEE----DKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHD 300

Query: 302 TTSILLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLR 361
           TTSIL+TLMLRV ATNPTVYA VLQEHE+I RSKECGE L WEDVSKMKYTWRVALETLR
Sbjct: 301 TTSILITLMLRVMATNPTVYAAVLQEHEDIHRSKECGEALTWEDVSKMKYTWRVALETLR 360

Query: 362 LFPPVFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDK-QTS 421
           LFPPVFGGFRVALKDI+ GGYTIPKGWQIFWAA MTHLDE IF DPQKF+PSRFD+ QTS
Sbjct: 361 LFPPVFGGFRVALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFTDPQKFKPSRFDQNQTS 420

Query: 422 I-PPFSYIAFGGGPRICPGNEFAKLETLVTIHYLITQFTWKLSSSQDFLTRDPTLTPNKG 481
           I PP S+IAFGGGPRICPG EFAKLETLVTIHY+ITQFTWKL SSQDFLTRDP+L PNKG
Sbjct: 421 IPPPLSFIAFGGGPRICPGYEFAKLETLVTIHYIITQFTWKL-SSQDFLTRDPSLMPNKG 478

Query: 482 LPIQIFPK 488
           LPIQ++PK
Sbjct: 481 LPIQLYPK 478

BLAST of Lag0038028 vs. ExPASy TrEMBL
Match: A0A0A0L864 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G416660 PE=3 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.7e-221
Identity = 398/483 (82.40%), Postives = 427/483 (88.41%), Query Frame = 0

Query: 6   ITLLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSAEEWL 65
           IT+++L   T  L  R R  LSS  +AAKNLPPG LGVPFIGQSLSLL AMR N+AE+WL
Sbjct: 2   ITVIILLVLTIFLFHRFRTRLSS--SAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWL 61

Query: 66  QQRVAKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGERNLTE 125
           Q+RV KYG VSKM LFGKPTVF+ GAAAN+AVVFSGEE  VSN Q ESL+ ILG+RNLTE
Sbjct: 62  QKRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTE 121

Query: 126 LSGEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTLTFDI 185
           LSGEDHKRVRGA+VWFLRPQ LR +V KMDGEVR+HLNMYWH NN+V V PLMKTLTFDI
Sbjct: 122 LSGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDI 181

Query: 186 ICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQLLKE 245
           ICSLLFGIEEG TRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAK QQ+LKQLLK+
Sbjct: 182 ICSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKD 241

Query: 246 RRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHDTTSI 305
           + + MEEE+E   E E+  KDLI+YLL +KNKDKEQALSEEEIVHNII+LMIAGHDTTSI
Sbjct: 242 KAKVMEEEKE--SEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSI 301

Query: 306 LLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLRLFPP 365
           LLTLMLRV ATNPTVYA VLQEHEEI RSKE GE L WEDVSKMKYTWRVA+ETLRL+PP
Sbjct: 302 LLTLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPP 361

Query: 366 VFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDK-QTSIPPF 425
           VFGGFRVALKDI+ G YTIPKGWQIFWAAPMTHLDETIF DPQKFEPSRFD+ QT IPPF
Sbjct: 362 VFGGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPF 421

Query: 426 SYIAFGGGPRICPGNEFAKLETLVTIHYLITQFTWKLSSSQDFLTRDPTLTPNKGLPIQI 485
           S+IAFGGGPRICPG EFAKLETLVTIHYLITQFTW LS SQDFLTRDPTL PNKGLPIQI
Sbjct: 422 SFIAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQI 480

Query: 486 FPK 488
           FPK
Sbjct: 482 FPK 480

BLAST of Lag0038028 vs. ExPASy TrEMBL
Match: A0A1S3C037 (LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103494945 PE=3 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 3.3e-217
Identity = 395/487 (81.11%), Postives = 428/487 (87.89%), Query Frame = 0

Query: 2   MIITITLLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSA 61
           MI  I LL++T   FL  R R +  SS   AAKNLPPG LGVPFIGQSLSLL AMR N+A
Sbjct: 1   MITPIILLVIT--IFLFHRFRTRFSSS---AAKNLPPGALGVPFIGQSLSLLGAMRTNTA 60

Query: 62  EEWLQQRVAKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGER 121
           E+WLQQRV KYG VSKM LFGKPTVFV GAAAN+AVVFSGEE  VSN Q ESL+ ILG+R
Sbjct: 61  EKWLQQRVDKYGPVSKMTLFGKPTVFVHGAAANKAVVFSGEEGTVSNRQIESLKMILGKR 120

Query: 122 NLTELSGEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTL 181
           NLTELSGEDHKRVRGA+VWFLRPQ LR +V KMDGEVR+HLNMYWH NN+V V PLMKTL
Sbjct: 121 NLTELSGEDHKRVRGALVWFLRPQTLRSYVEKMDGEVRRHLNMYWHGNNKVTVAPLMKTL 180

Query: 182 TFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQ 241
           TFDIICSLLFGIEEG TRKSII+CFKTMV+GIWS+PINLPFTRYNHSLKASAKVQQ+LKQ
Sbjct: 181 TFDIICSLLFGIEEGTTRKSIIQCFKTMVEGIWSVPINLPFTRYNHSLKASAKVQQILKQ 240

Query: 242 LLKERRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHD 301
           LLK++ ++ME++++ K    KK KDLITYLL +KNKDKEQAL+EEEI+HNII+LMIAGHD
Sbjct: 241 LLKDKAKRMEQKKKMK----KKMKDLITYLLRIKNKDKEQALTEEEILHNIILLMIAGHD 300

Query: 302 TTSILLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLR 361
           TTSILLTLMLRV ATNPTVYA VLQEHEEIARSKE GE L WEDVSKMKYTWRVA+ETLR
Sbjct: 301 TTSILLTLMLRVLATNPTVYADVLQEHEEIARSKERGEALTWEDVSKMKYTWRVAMETLR 360

Query: 362 LFPPVFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDK-QTS 421
           L+PPVFGGFRVALKDI+ G YTIPKGWQIFWAA  THLDETIF DPQKFEPSRFD+ QT 
Sbjct: 361 LYPPVFGGFRVALKDIQLGAYTIPKGWQIFWAASTTHLDETIFGDPQKFEPSRFDQTQTP 420

Query: 422 IPPFSYIAFGGGPRICPGNEFAKLETLVTIHYLITQFTWKLSSSQDFLTRDPTLTPNKGL 481
           IPPFS+IAFGGGPRICPG EFAKLETLVTIHYLITQFTW LS SQD LTRDPTL PNKGL
Sbjct: 421 IPPFSFIAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDSLTRDPTLMPNKGL 478

Query: 482 PIQIFPK 488
           PIQIFPK
Sbjct: 481 PIQIFPK 478

BLAST of Lag0038028 vs. ExPASy TrEMBL
Match: A0A6J1CCZ3 (taxadiene 5-alpha hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111010356 PE=3 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 1.1e-212
Identity = 376/489 (76.89%), Postives = 428/489 (87.53%), Query Frame = 0

Query: 2   MIITITLLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSA 61
           MII +  L L T  FL    RR       T +  LPPGPLG+P+IGQSL LLRAMR+NSA
Sbjct: 1   MIIPMFFLFLLTIFFLFLTTRR-------TRSSRLPPGPLGLPYIGQSLGLLRAMRSNSA 60

Query: 62  EEWLQQRVAKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGER 121
           E+WL +RVAKYG VSKM LFGKPTVF+ G  AN+A VF+GEE A+SN QTESL+RILGER
Sbjct: 61  EQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANKA-VFTGEEGAMSNQQTESLKRILGER 120

Query: 122 NLTELSGEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTL 181
           NLTELSGEDHKRVRGA+V FL+P +L+++V KMDGE+RKHL MYWH N +V V PLMK L
Sbjct: 121 NLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMDGEIRKHLQMYWHDNKEVTVLPLMKIL 180

Query: 182 TFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQ 241
           TFDIICSLLFGIEEGP RKS++ CFKTMVDGIWS+PINLPFTRY+HSLKASAKVQQMLKQ
Sbjct: 181 TFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQMLKQ 240

Query: 242 LLKERRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHD 301
           LLKE+R+KME+E++E+E+     +DL+T LLS+KNKDKEQALSE+EIVHNIIILM+AGHD
Sbjct: 241 LLKEKREKMEKEDQEEEQ-----QDLVTCLLSMKNKDKEQALSEDEIVHNIIILMVAGHD 300

Query: 302 TTSILLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLR 361
           TT+IL+TLM+RV  +NP VYA VLQEHEEIARSKE GEPL+WEDV KMKYTWRVALETLR
Sbjct: 301 TTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEPLSWEDVGKMKYTWRVALETLR 360

Query: 362 LFPPVFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDKQTSI 421
           LFPPVFGGFRVAL+DIEFGGYTIPKGWQ+FWAAPMTHLD+TIFADP KFEP+RFDKQT I
Sbjct: 361 LFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLDDTIFADPHKFEPNRFDKQTLI 420

Query: 422 PPFSYIAFGGGPRICPGNEFAKLETLVTIHYLITQFTWKLSSSQDFLTRDPTLTPNKGLP 481
           PP  +IAFGGGPRICPG+EFAKLETL+TIHYLITQFTWKL  S++FLTRDPTL PNKGLP
Sbjct: 421 PPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFTWKL-CSEEFLTRDPTLMPNKGLP 475

Query: 482 IQIFPKQTS 491
           IQI+PK+TS
Sbjct: 481 IQIYPKETS 475

BLAST of Lag0038028 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 316.6 bits (810), Expect = 3.5e-86
Identity = 167/478 (34.94%), Postives = 284/478 (59.41%), Query Frame = 0

Query: 11  LTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSA-EEWLQQRV 70
           +++  F  R++  + ++      K L PG +G+P+IG+++   +A ++N   E+++  R+
Sbjct: 20  ISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRI 79

Query: 71  AKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGERNLTELSGE 130
            K+G + K  + G PT+ V+GA ANR ++ S E + V ++   S  +++G   +    GE
Sbjct: 80  IKHGNIFKTRIMGSPTIVVNGAEANR-LILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGE 139

Query: 131 DHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTLTFDIICSL 190
            H+ +RG V   L    L   + K+   V+ H    W    ++ +    K LTF ++   
Sbjct: 140 KHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFEC 199

Query: 191 LFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQLLKERRQK 250
           L+GI+       ++E F+ +++G++++P+  P +++  + KA  +++  L   ++E+R++
Sbjct: 200 LYGIK---VEIGMLEVFERVLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKRRE 259

Query: 251 MEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHDTTSILLTL 310
           M     EKE  EK +  L + L+    K     ++EEE+V N+++L+ A HDTTS  +++
Sbjct: 260 M-----EKEGAEKPNTTLFSRLVEELIKG---VITEEEVVDNMVLLVFAAHDTTSYAMSM 319

Query: 311 MLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLRLFPPVFGG 370
             ++ A +PT   T+LQEH +I  +K  GE L  EDV KMKY+W+V  ET+RL PP+FG 
Sbjct: 320 TFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGS 379

Query: 371 FRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDKQTSIPPFSYIAF 430
           FR A+ DI++GGYTIPKGW+I W    TH +  IF DP  F+P+RFDK   I  ++Y+ F
Sbjct: 380 FRKAVADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDK--PIQAYTYLPF 439

Query: 431 GGGPRICPGNEFAKLETLVTIHYLITQFTWKLSSSQDFLTRDPTLTPNKGLPIQIFPK 488
           GGGPR+C G++ AK+  LV +H+++T F W L    + ++ DP   P+ G+PI+I PK
Sbjct: 440 GGGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISPK 483

BLAST of Lag0038028 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 315.5 bits (807), Expect = 7.7e-86
Identity = 180/492 (36.59%), Postives = 294/492 (59.76%), Query Frame = 0

Query: 1   MMIITITLLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANS 60
           M +  + +L L++    L    RK LS    +  NLPPG  G+P IG+S S L A R   
Sbjct: 1   MYMAIMIILFLSSILLSLLLLLRKHLSH--FSYPNLPPGNTGLPLIGESFSFLSAGRQGH 60

Query: 61  AEEWLQQRVAKYGA----VSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRR 120
            E+++  RV ++ +    V K  LFG PT  V+GA+ N+  +F+ E   V +   +S+ +
Sbjct: 61  PEKFITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNK-FLFTNENKLVVSWWPDSVNK 120

Query: 121 ILGERNLTELSGEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNP 180
           I      T  S E+ +++R  +  F++P+ LR +V  MD   ++H    W   +QVIV P
Sbjct: 121 IFPSSMQTS-SKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFP 180

Query: 181 LMKTLTFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQ 240
           L K  TF I C     +E+    + + E F T+  GI+SIPI+LP TR+N ++KAS  ++
Sbjct: 181 LTKKFTFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLR 240

Query: 241 QMLKQLLKERRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILM 300
           + +  ++++R+++++  +  +E       D+++++L    + K     +E++   II L+
Sbjct: 241 KEVSAIVRQRKEELKAGKALEEH------DILSHMLMNIGETK-----DEDLADKIIGLL 300

Query: 301 IAGHDTTSILLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVA 360
           I GHDT SI+ T ++   A  P VY  VLQE +EI + K+  E L WED+ KM+Y+W VA
Sbjct: 301 IGGHDTASIVCTFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVA 360

Query: 361 LETLRLFPPVFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFD 420
            E +R+ PP+ G FR A+    F G+ IPKGW+++W+A  TH++   F +P++FEP+RF+
Sbjct: 361 CEVMRIVPPLSGTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFE 420

Query: 421 KQTSIPPFSYIAFGGGPRICPGNEFAKLETLVTIHYLITQFTW-KLSSSQDFLTRDPTLT 480
             +   P++Y+ FGGGPR+CPG E+A+LE L+ +H L+ +F W K+  +++ +  DP   
Sbjct: 421 -GSGPKPYTYVPFGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPI 476

Query: 481 PNKGLPIQIFPK 488
           P+KGLPI+IFP+
Sbjct: 481 PDKGLPIRIFPQ 476

BLAST of Lag0038028 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 243.4 bits (620), Expect = 3.7e-64
Identity = 161/486 (33.13%), Postives = 260/486 (53.50%), Query Frame = 0

Query: 2   MIITITLLLLTT--ATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRAN 61
           M  T  LLLL++  A FLL  RR      T      LPPG LG+P IG++  L+ A +  
Sbjct: 1   MAFTAFLLLLSSIAAGFLLLLRR------TRYRRMGLPPGSLGLPLIGETFQLIGAYKTE 60

Query: 62  SAEEWLQQRVAKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILG 121
           + E ++ +RVA+YG+V    LFG+PT+F +    NR  V   E      +   S+  +LG
Sbjct: 61  NPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR-FVLQNEGKLFECSYPASICNLLG 120

Query: 122 ERNLTELSGEDHKRVRGAVVWFLRPQNLRE-FVRKMDGEVRKHLNMYWHPNNQVIVNPLM 181
           + +L  + G  HKR+    + F     +++  +  +D  VR +L+ +   +++V++    
Sbjct: 121 KHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSW---SSRVLLMEEA 180

Query: 182 KTLTFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQM 241
           K +TF++    L   + G   +S+ + +  +++G +S+P+ L  T Y  +++A  KV + 
Sbjct: 181 KKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEA 240

Query: 242 LKQLLKERRQKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIA 301
           L  ++ +RR       EE+EEG ++ KD++  LL+      +   S+EEIV  ++ L++A
Sbjct: 241 LTVVVMKRR-------EEEEEGAERKKDMLAALLA-----ADDGFSDEEIVDFLVALLVA 300

Query: 302 GHDTTSILLTLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALE 361
           G++TTS ++TL ++     P   A + +EHE+I   K     L W D   M +T  V  E
Sbjct: 301 GYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNE 360

Query: 362 TLRLFPPVFGGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDKQ 421
           TLR+   + G FR A+ D+E  GY IPKGW++F +    HLD   F D + F P R+   
Sbjct: 361 TLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSN 420

Query: 422 --TSIPPFSYIAFGGGPRICPGNEFAKLETLVTIHYLITQFTWKLSSSQDFLTRDPTLTP 481
             T+ P   +  FGGGPR+CPG E A++   V +H L+T F+W + + QD L   PT   
Sbjct: 421 SVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGFSW-VPAEQDKLVFFPTTRT 463

Query: 482 NKGLPI 483
            K  PI
Sbjct: 481 QKRYPI 463

BLAST of Lag0038028 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 235.3 bits (599), Expect = 1.0e-61
Identity = 141/450 (31.33%), Postives = 248/450 (55.11%), Query Frame = 0

Query: 37  PPGPLGVPFIGQSLSLLRAMRANSAEEWLQQRVAKYGAVSKMGLFGKPTVFVSGAAANRA 96
           P G LG P IG+++  + +  ++  E ++ +R   YG V K  +FG  T+  + A  NRA
Sbjct: 52  PHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRA 111

Query: 97  VVFSGEEAAVSNAQTESLRRILGERNLTELSGEDHKRVRGAVVWFLRPQNLR-EFVRKMD 156
           V+ S   A V     +++R ++G+ ++  ++G  H+R  G V  FL+   L+ + VR M 
Sbjct: 112 VLQSDSTAFVP-FYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMH 171

Query: 157 GEVRKHLNMYWHPNNQVIVNPLMKTLTFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWS 216
             + + +++ W  +  V++  + KT+ F ++   L  +E+G   + +   F+  + G+ S
Sbjct: 172 KFLSESMDL-WSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMS 231

Query: 217 IPINLPFTRYNHSLKASAKVQQMLKQLLKERRQKMEEEEEEKEEGEKKHKDLITYLLSLK 276
           +PIN P T+ + SL+A   + + ++++++ + +K     + KEE +   KD++  LL   
Sbjct: 232 LPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRK----TKNKEEDDVIAKDVVDVLL--- 291

Query: 277 NKDKEQALSEEEIVHNIIILMIAGHDTTSILLTLMLRVFATNPTVYATVLQEHEEIARSK 336
            KD  + L+   I +N+I +MI GHD+  +L+TL ++  + +P     + +E+ ++   K
Sbjct: 292 -KDSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLK 351

Query: 337 E-CGEPLNWEDVSKMKYTWRVALETLRLFPPVFGGFRVALKDIEFGGYTIPKGWQIFWAA 396
           E  GEPL W D   + +T +V  ETLR+   + G  R A+KD+E  GY IPKGW      
Sbjct: 352 ELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYL 411

Query: 397 PMTHLDETIFADPQKFEPSRFDKQTSIPPFSYIAFGGGPRICPGNEFAKLETLVTIHYLI 456
              HLD+  +  P KF P R+ ++  +   S+  FGGG R+CPG + A+LET V +H+L+
Sbjct: 412 RSVHLDKLYYESPYKFNPWRW-QERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLV 471

Query: 457 TQFTWKLSSSQDFLTRDPTLTPNKGLPIQI 485
           T+F W   + +D +   PT+     LPI I
Sbjct: 472 TRFRW--IAEEDTIINFPTVHMKNKLPIWI 488

BLAST of Lag0038028 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 235.0 bits (598), Expect = 1.3e-61
Identity = 152/477 (31.87%), Postives = 243/477 (50.94%), Query Frame = 0

Query: 8   LLLLTTATFLLRRRRRKSLSSTATAAKNLPPGPLGVPFIGQSLSLLRAMRANSAEEWLQQ 67
           L L   A FL   R    +  +++    LPPG +G P++G++  L     +     +   
Sbjct: 8   LTLSAAALFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLY----SQDPNVFFAA 67

Query: 68  RVAKYGAVSKMGLFGKPTVFVSGAAANRAVVFSGEEAAVSNAQTESLRRILGERNLTELS 127
           +  +YG+V K  + G P V +S   A + V+ + +          S  R+LG++ +    
Sbjct: 68  KQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVT-KSHLFKPTFPASKERMLGKQAIFFHQ 127

Query: 128 GEDHKRVRGAVVWFLRPQNLREFVRKMDGEVRKHLNMYWHPNNQVIVNPLMKTLTFDIIC 187
           G+ H ++R  V+    P  +R  V  ++   ++ LN +     Q+     MKT TF++  
Sbjct: 128 GDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNVAL 187

Query: 188 SLLFGIEEGPTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKVQQMLKQLLKERR 247
             + G +E   R+ +  C+  +  G  S+PINLP T ++ ++KA  ++ Q+L  +L +RR
Sbjct: 188 ISILGKDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRR 247

Query: 248 QKMEEEEEEKEEGEKKHKDLITYLLSLKNKDKEQALSEEEIVHNIIILMIAGHDTTSILL 307
           Q               H DL+   +     + +  L++E+I  NII ++ A  DTT+ +L
Sbjct: 248 Q-----------NPSSHTDLLGSFM-----EDKAGLTDEQIADNIIGVIFAARDTTASVL 307

Query: 308 TLMLRVFATNPTVYATVLQEHEEIARSKECGEPLNWEDVSKMKYTWRVALETLRLFPPVF 367
           T +L+  A NPTV   V +E   I + K+ GE L WED  KM  T+RV  ETLR    + 
Sbjct: 308 TWILKYLADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILS 367

Query: 368 GGFRVALKDIEFGGYTIPKGWQIFWAAPMTHLDETIFADPQKFEPSRFDKQTSIPPFSYI 427
             FR A++D+E+ GY IPKGW++       H +  IF+DP KF+PSRF  + +  P +++
Sbjct: 368 FTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRF--EVAPKPNTFM 427

Query: 428 AFGGGPRICPGNEFAKLETLVTIHYLITQFTWKLSSSQDFLTRDPTLTPNKGLPIQI 485
            FG G   CPGNE AKLE  V IH+L T++ W +    D +   P   P  GLPI +
Sbjct: 428 PFGSGIHSCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIAL 459