Lag0037968 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0037968
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Description3-epi-6-deoxocathasterone 23-monooxygenase
Locationchr2: 11127507 .. 11128956 (+)
RNA-Seq ExpressionLag0037968
SyntenyLag0037968
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCAGTATCAATTAAGCAACCGACTGCGGACGATAATTTGTTGGTCATTACATCACCTGGTTCTCATTGCTACATTATCGATGGTGACGAAGAGTTAGTACTTCTGCTTTCAATATTGATTTCCAATTGTTATGAATGTATCTCTGTCTTTTCCTCAAGCTCAGGCGCCTAATGATTTTATATGTCTTAAATCTTCTGAATTGAGTTGTTGTATTGCTTGCTTTGTTAATTACTTTCGTGATGTACTTAATTTACTTTCTGCTGTTTTTAGTCAAGTAATTCTAGGGATTTTAAAGCGTTTGATGCAAATTCAATCTATGCACCTTAATCCTTCAGTCCGATGTTTGATTTATCCAGTCTTAACAGTAGTTCGAATTAGAGTTCTTATCCTTTCCGCGTCAAACATCTGATGTGAAATTATGTTATGTCATATGTGCAGATCATTTGTTCTTTCTTGCGTTCTGCAGATAAAATTCTTTACTGAAGTTAATTCATGCCACAAGCGGTGAGATTTGGGCAAGTGGCTGAATTACGTTATGCCCTGACACACTTCAATCTTTGAATATCTTAGAGGTCAAAAGGTCGCTTAGCTTATTACACTTTTTACGCTACTGAGGTAACTCGTGAAAATCCTCAGCTTGGAAACGGCATAAATAGACGATTATCAGCTTGGAAAAACTGGGACGGGACGACCCATTTCCTGGAACCTTGACCATGGAAATAGAGAATCATAGCTTCCCAGAACCAGCCATGTGGATCGGTACCCAAGAGTACATGAGAAGTGGACCTTGGAATGGGCTTCGGTACTGTGACCATTCCCTTTCCAAAAATGTCCCAACTCTCCTCTTTTCATTCCAACTCTAGAAGAAAGGGTAGCCACTCTCAATTAGTGAAAAAAGATTGAAAGTTGAAAAAATGGAAGTTTGGTCTGTTAGGTAGAATAGAGGATTAGCCTAGTTAATCACCTAGAAGACAAAATTGATGGCAAGCCTACCGAATGGTTCCATTTCATTGCTTGTAAATAATCACAGCCCCTCAGTTTCAGCTTTCCCATAAGAAAAAAAATACTGCAATTGAAATCAAATGACCATTCCCTTTCCAAAAATGTCCAAACTCTCCTTTTTTTCGCTCCAAGTCTACAAGAAAGAGGAAGATTGAAAGTTGAAAGCTTTTTGAGCTTAAAATTTGTGGGTTTTTTTTGCAGGTACGCCAACGTGTTTAAAACCAACATTTTGGGGATACCCATCATAGTTTCCAGTACTGATTCAGACACAAATAAGGTGGTTTTACAAAACCATGGCAACATTTTTATCCCAGCGAACCCCAAATCAATTACCGAATCACTAATTGGTTCATTTTCAATTCTTCAAATGAATGGAAATGTTCATAAAAGAGTTCATACAATCATTGGAGCTTTTCTTAGGTCTCCTCAGCACAAAGCCTGA

mRNA sequence

ATGGCGGCAGTATCAATTAAGCAACCGACTGCGGACGATAATTTGTTGGTCATTACATCACCTGGTTCTCATTGCTACATTATCGATGGTGACGAAGAATCATTTGTTCTTTCTTGCGTTCTGCAGATAAAATTCTTTACTGAAGTTAATTCATGCCACAAGCGCTTGGAAAAACTGGGACGGGACGACCCATTTCCTGGAACCTTGACCATGGAAATAGAGAATCATAGCTTCCCAGAACCAGCCATGTGGATCGGTACCCAAGAGTACATGAGAAGTGGACCTTGGAATGGGCTTCGGTACGCCAACGTGTTTAAAACCAACATTTTGGGGATACCCATCATAGTTTCCAGTACTGATTCAGACACAAATAAGGTGGTTTTACAAAACCATGGCAACATTTTTATCCCAGCGAACCCCAAATCAATTACCGAATCACTAATTGGTTCATTTTCAATTCTTCAAATGAATGGAAATGTTCATAAAAGAGTTCATACAATCATTGGAGCTTTTCTTAGGTCTCCTCAGCACAAAGCCTGA

Coding sequence (CDS)

ATGGCGGCAGTATCAATTAAGCAACCGACTGCGGACGATAATTTGTTGGTCATTACATCACCTGGTTCTCATTGCTACATTATCGATGGTGACGAAGAATCATTTGTTCTTTCTTGCGTTCTGCAGATAAAATTCTTTACTGAAGTTAATTCATGCCACAAGCGCTTGGAAAAACTGGGACGGGACGACCCATTTCCTGGAACCTTGACCATGGAAATAGAGAATCATAGCTTCCCAGAACCAGCCATGTGGATCGGTACCCAAGAGTACATGAGAAGTGGACCTTGGAATGGGCTTCGGTACGCCAACGTGTTTAAAACCAACATTTTGGGGATACCCATCATAGTTTCCAGTACTGATTCAGACACAAATAAGGTGGTTTTACAAAACCATGGCAACATTTTTATCCCAGCGAACCCCAAATCAATTACCGAATCACTAATTGGTTCATTTTCAATTCTTCAAATGAATGGAAATGTTCATAAAAGAGTTCATACAATCATTGGAGCTTTTCTTAGGTCTCCTCAGCACAAAGCCTGA

Protein sequence

MAAVSIKQPTADDNLLVITSPGSHCYIIDGDEESFVLSCVLQIKFFTEVNSCHKRLEKLGRDDPFPGTLTMEIENHSFPEPAMWIGTQEYMRSGPWNGLRYANVFKTNILGIPIIVSSTDSDTNKVVLQNHGNIFIPANPKSITESLIGSFSILQMNGNVHKRVHTIIGAFLRSPQHKA
Homology
[Close]
BLAST of Lag0037968 vs. NCBI nr
Match: XP_038881879.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 [Benincasa hispida])

HSP 1 Score: 125.9 bits (315), Expect = 3.4e-25
Identity = 66/79 (83.54%), Postives = 71/79 (89.87%), Query Frame = 0 

Query: 101 YANVFKTNILGIPIIVSSTDSDTNKVVLQNHGNIFIPANPKSITESLIGSFSILQMNGNV 160
           Y NVFKTNILG PIIV STDS+ NKVVLQNHGNIFIPA PKSITE L+G+FSIL+MNGNV
Sbjct: 72  YGNVFKTNILGTPIIV-STDSEVNKVVLQNHGNIFIPAYPKSITE-LLGTFSILRMNGNV 131

Query: 161 HKRVHTIIGAFLRSPQHKA 180
            KRVHT+IGAFLRSPQ KA
Sbjct: 132 QKRVHTLIGAFLRSPQLKA 148

[Close]
BLAST of Lag0037968 vs. NCBI nr
Match: KAG7026770.1 (3-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 119.8 bits (299), Expect = 2.4e-23
Identity = 62/79 (78.48%), Postives = 68/79 (86.08%), Query Frame = 0 

Query: 101 YANVFKTNILGIPIIVSSTDSDTNKVVLQNHGNIFIPANPKSITESLIGSFSILQMNGNV 160
           Y +VFKTNILG PIIV STDSD NKVVLQNHGN F+PA PKSITE L+GSFSILQMNGN 
Sbjct: 79  YGSVFKTNILGTPIIV-STDSDVNKVVLQNHGNTFVPAYPKSITE-LLGSFSILQMNGNF 138

Query: 161 HKRVHTIIGAFLRSPQHKA 180
            KR+H+IIGAFL+SP  KA
Sbjct: 139 QKRIHSIIGAFLKSPSLKA 155

[Close]
BLAST of Lag0037968 vs. NCBI nr
Match: XP_023518560.1 (3-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 119.8 bits (299), Expect = 2.4e-23
Identity = 62/79 (78.48%), Postives = 68/79 (86.08%), Query Frame = 0 

Query: 101 YANVFKTNILGIPIIVSSTDSDTNKVVLQNHGNIFIPANPKSITESLIGSFSILQMNGNV 160
           Y +VFKTNILG PIIV STDSD NKVVLQNHGN F+PA PKSITE L+GSFSILQMNGN 
Sbjct: 76  YGSVFKTNILGTPIIV-STDSDVNKVVLQNHGNTFVPAYPKSITE-LLGSFSILQMNGNF 135

Query: 161 HKRVHTIIGAFLRSPQHKA 180
            KR+H+IIGAFL+SP  KA
Sbjct: 136 QKRIHSIIGAFLKSPSLKA 152

[Close]
BLAST of Lag0037968 vs. NCBI nr
Match: KAG6594802.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 119.8 bits (299), Expect = 2.4e-23
Identity = 62/79 (78.48%), Postives = 68/79 (86.08%), Query Frame = 0 

Query: 101 YANVFKTNILGIPIIVSSTDSDTNKVVLQNHGNIFIPANPKSITESLIGSFSILQMNGNV 160
           Y +VFKTNILG PIIV STDSD NKVVLQNHGN F+PA PKSITE L+GSFSILQMNGN 
Sbjct: 80  YGSVFKTNILGTPIIV-STDSDVNKVVLQNHGNTFVPAYPKSITE-LLGSFSILQMNGNF 139

Query: 161 HKRVHTIIGAFLRSPQHKA 180
            KR+H+IIGAFL+SP  KA
Sbjct: 140 QKRIHSIIGAFLKSPSLKA 156

[Close]
BLAST of Lag0037968 vs. NCBI nr
Match: XP_022963240.1 (3-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita moschata])

HSP 1 Score: 119.0 bits (297), Expect = 4.2e-23
Identity = 61/79 (77.22%), Postives = 68/79 (86.08%), Query Frame = 0 

Query: 101 YANVFKTNILGIPIIVSSTDSDTNKVVLQNHGNIFIPANPKSITESLIGSFSILQMNGNV 160
           Y +VFKTNILG PIIV STDSD NKVVLQNHGN F+PA PKSITE L+GSFSILQMNGN 
Sbjct: 76  YGSVFKTNILGTPIIV-STDSDVNKVVLQNHGNTFVPAYPKSITE-LLGSFSILQMNGNF 135

Query: 161 HKRVHTIIGAFLRSPQHKA 180
            KR+H++IGAFL+SP  KA
Sbjct: 136 QKRIHSLIGAFLKSPSLKA 152

[Close]
BLAST of Lag0037968 vs. ExPASy Swiss-Prot
Match: Q9M066 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=3702 GN=CYP90C1 PE=1 SV=3)

HSP 1 Score: 102.8 bits (255), Expect = 4.0e-21
Identity = 53/78 (67.95%), Postives = 62/78 (79.49%), Query Frame = 0 

Query: 101 YANVFKTNILGIPIIVSSTDSDTNKVVLQNHGNIFIPANPKSITESLIGSFSILQMNGNV 160
           Y  VFKTNI+G PII+ STD++ NKVVLQNHGN F+PA PKSITE L+G  SIL +NG  
Sbjct: 105 YGKVFKTNIIGTPIII-STDAEVNKVVLQNHGNTFVPAYPKSITE-LLGENSILSINGPH 164

Query: 161 HKRVHTIIGAFLRSPQHK 179
            KR+HT+IGAFLRSP  K
Sbjct: 165 QKRLHTLIGAFLRSPHLK 180

[Close]
BLAST of Lag0037968 vs. ExPASy Swiss-Prot
Match: Q94IA6 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=3702 GN=CYP90D1 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.2e-12
Identity = 38/81 (46.91%), Postives = 56/81 (69.14%), Query Frame = 0 

Query: 99  LRYANVFKTNILGIPIIVSSTDSDTNKVVLQNHGNIFIPANPKSITESLIGSFSILQMNG 158
           L Y  VFK++I G   IV STD++ N+ VLQ+    F+P  PK++ E L+G  SIL +NG
Sbjct: 85  LMYGRVFKSHIFGTATIV-STDAEVNRAVLQSDSTAFVPFYPKTVRE-LMGKSSILLING 144

Query: 159 NVHKRVHTIIGAFLRSPQHKA 180
           ++H+R H ++G+FL+SP  KA
Sbjct: 145 SLHRRFHGLVGSFLKSPLLKA 163

[Close]
BLAST of Lag0037968 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 4.6e-09
Identity = 33/75 (44.00%), Postives = 50/75 (66.67%), Query Frame = 0 

Query: 100 RYANVFKTNILGIPIIVSSTDSDTNKVVLQNHGNIFIPANPKSITESLIGSFSILQMNGN 159
           RY +VF T++ G P I S+ D +TN+ VLQN G +F  + P SI  +L+G  S+L M G+
Sbjct: 66  RYGSVFMTHLFGEPTIFSA-DPETNRFVLQNEGKLFECSYPASIC-NLLGKHSLLLMKGS 125

Query: 160 VHKRVHTIIGAFLRS 175
           +HKR+H++  +F  S
Sbjct: 126 LHKRMHSLTMSFANS 138

[Close]
BLAST of Lag0037968 vs. ExPASy Swiss-Prot
Match: Q94IW5 (Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 8.7e-08
Identity = 29/76 (38.16%), Postives = 52/76 (68.42%), Query Frame = 0 

Query: 104 VFKTNILGIPIIVSSTDSDTNKVVLQNHGNIFIPANPKSITESLIGSFSILQMNGNVHKR 163
           VF++++ G   +V++ D++ ++ VLQ+    F+P  P+S+TE L+G  SIL +NG + +R
Sbjct: 89  VFRSHLFGSATVVTA-DAEVSRFVLQSDARAFVPWYPRSLTE-LMGKSSILLINGALQRR 148

Query: 164 VHTIIGAFLRSPQHKA 180
           VH ++GAF +S   K+
Sbjct: 149 VHGLVGAFFKSSHLKS 162

[Close]
BLAST of Lag0037968 vs. ExPASy Swiss-Prot
Match: Q50EK5 (Cytochrome P450 720B2 OS=Pinus taeda OX=3352 GN=CYP720B2 PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 4.1e-05
Identity = 24/66 (36.36%), Postives = 41/66 (62.12%), Query Frame = 0 

Query: 100 RYANVFKTNILGIPIIVSSTDSDTNKVVLQNHGNIFIPANPKSITESLIGSFSILQMNGN 159
           RY  +F++N+ G   IV S D + NK VLQ+ G  F    PK +  +LIG + +L ++G+
Sbjct: 80  RYGEIFRSNLFGRSRIVVSVDPEFNKHVLQHEGRQFQANYPKPL-RNLIGKYGLLSVHGD 139

Query: 160 VHKRVH 166
           + +++H
Sbjct: 140 LQRKLH 144

The following BLAST results are available for this feature:
BLAST of Lag0037968 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038881879.13.4e-2583.543-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 [Benincasa hispida][more]
KAG7026770.12.4e-2378.483-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita argyrosperma subsp. argyro... [more]
XP_023518560.12.4e-2378.483-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita pepo subsp. pepo][more]
KAG6594802.12.4e-2378.483-epi-6-deoxocathasterone 23-monooxygenase CYP90C1, partial [Cucurbita argyrospe... [more]
XP_022963240.14.2e-2377.223-epi-6-deoxocathasterone 23-monooxygenase [Cucurbita moschata][more]
BLAST of Lag0037968 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M0664.0e-2167.953-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=37... [more]
Q94IA61.2e-1246.913-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=37... [more]
Q425694.6e-0944.00Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1[more]
Q94IW58.7e-0838.16Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV... [more]
Q50EK54.1e-0536.36Cytochrome P450 720B2 OS=Pinus taeda OX=3352 GN=CYP720B2 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 76..179
e-value: 2.4E-7
score: 31.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 100..175
NoneNo IPR availablePANTHERPTHR24286:SF2543-EPI-6-DEOXOCATHASTERONE 23-MONOOXYGENASE CYP90C1coord: 99..179
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 99..179

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0037968.1Lag0037968.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0009965 leaf morphogenesis
biological_process GO:0042814 monopolar cell growth
biological_process GO:0048441 petal development
biological_process GO:0048443 stamen development
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen