Lag0037874 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0037874 vs. NCBI nr
Match: XP_023002177.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002870.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002871.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 471.5 bits (1212), Expect = 6.4e-129
Identity = 225/289 (77.85%), Postives = 254/289 (87.89%), Query Frame = 0

Query: 1   MALLRLSILLCMMLFGPSLSGATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPP 60
           M LL+LS LLCM+LF PSLS AT+P NIFILAGQSNMAGRGGVEN++ GK L WD  VP 
Sbjct: 1   MILLKLSTLLCMILFHPSLSWATSPTNIFILAGQSNMAGRGGVENNQKGK-LEWDGKVPL 60

Query: 61  MCQPDPSILRLNPQLQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGL 120
            CQ DPSILRLNP  QWEIA+EP+H GID+  KTPGIGP +  A Q +AK GQKAG+VGL
Sbjct: 61  ECQSDPSILRLNPARQWEIAQEPLHLGIDI-GKTPGIGPGIPFAHQFKAKAGQKAGIVGL 120

Query: 121 VPCAKGGTLIKQWMKNPSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTA 180
           VPCA+GGTLI+QW+KNPSNP+ATFYQNFIERIK S+KEGGVVRALFW+ GESDAAMSDTA
Sbjct: 121 VPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTA 180

Query: 181 HKYKDNLKKFFTDVRNDIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPD 240
           H+YKDNLKKF TD+RNDIKPRFLP+IIVK ++YDFFMKHDTHDLPAVRAA++A+Q ELPD
Sbjct: 181 HRYKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPD 240

Query: 241 VVTIDSWKLPINLNTHEGFNLDHGHFNTATEIALGKWLADTYLSHYGFL 290
           ++TIDSW+LPIN  T EGF LDHGHFNTATEIALGKWLADTYL+HY  L
Sbjct: 241 IITIDSWELPINFTTFEGFCLDHGHFNTATEIALGKWLADTYLAHYSHL 287

BLAST of Lag0037874 vs. NCBI nr
Match: XP_023536885.1 (probable carbohydrate esterase At4g34215 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023536886.1 probable carbohydrate esterase At4g34215 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 465.7 bits (1197), Expect = 3.5e-127
Identity = 221/289 (76.47%), Postives = 252/289 (87.20%), Query Frame = 0

Query: 1   MALLRLSILLCMMLFGPSLSGATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPP 60
           M LL+LSILLCM+LF PSLSGAT+P NIFILAGQSNMAGRGGVE  ++GK LVWD  VP 
Sbjct: 1   MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGK-LVWDGKVPL 60

Query: 61  MCQPDPSILRLNPQLQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGL 120
            CQ DPSILRLNP+ QWEIA EP+H GID+   TPGIGP +  A Q + K GQKAG VGL
Sbjct: 61  ECQSDPSILRLNPERQWEIAHEPLHLGIDI-SHTPGIGPGIPFAHQFKEKAGQKAGTVGL 120

Query: 121 VPCAKGGTLIKQWMKNPSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTA 180
           VPCA+GGTLI+QW+KNPSNP+ATFYQNFIERIK S+KEGGVVRALFW+ GESDAAM+DTA
Sbjct: 121 VPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTA 180

Query: 181 HKYKDNLKKFFTDVRNDIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPD 240
            +YKDNLKKF TD+RNDIKPRFLP+IIVK ++YDFFMKHDTH+LPAVRAA++A+Q ELPD
Sbjct: 181 QRYKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPD 240

Query: 241 VVTIDSWKLPINLNTHEGFNLDHGHFNTATEIALGKWLADTYLSHYGFL 290
           ++TIDSW+LPINL T EGF+ DHGHFNT TEI LGKWLADTYL+HYG L
Sbjct: 241 IITIDSWELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHL 287

BLAST of Lag0037874 vs. NCBI nr
Match: XP_038886442.1 (probable carbohydrate esterase At4g34215 [Benincasa hispida])

HSP 1 Score: 464.9 bits (1195), Expect = 5.9e-127
Identity = 223/289 (77.16%), Postives = 251/289 (86.85%), Query Frame = 0

Query: 1   MALLRLSILLCMMLFGPSLSGATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPP 60
           MALL+LSILLC MLFG SLS A +P NIFILAGQSNMAGRGGVEN++  + L WD  +PP
Sbjct: 1   MALLKLSILLCTMLFGSSLSRAASPNNIFILAGQSNMAGRGGVENNQV-RELEWDGLIPP 60

Query: 61  MCQPDPSILRLNPQLQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGL 120
            CQ DPSILRLNP LQWEIAREP+H GID+ +KT GIGP M  A Q+  KVG +AG VGL
Sbjct: 61  ECQSDPSILRLNPALQWEIAREPLHEGIDI-NKTVGIGPGMPFAHQLLTKVGPRAGTVGL 120

Query: 121 VPCAKGGTLIKQWMKNPSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTA 180
           VPCA+GGT+I+QW+KNPSNP+ATFY+NFIERIKASDKEGGVVRALFW  GESDAAMSDTA
Sbjct: 121 VPCARGGTIIEQWIKNPSNPDATFYKNFIERIKASDKEGGVVRALFWFQGESDAAMSDTA 180

Query: 181 HKYKDNLKKFFTDVRNDIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPD 240
           ++YKDNLK FFTD+RNDIKPRFLPII+VK  LYDF MKHDTHDLPAVRAAQ+A+  ELPD
Sbjct: 181 NRYKDNLKNFFTDIRNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAQDAVSKELPD 240

Query: 241 VVTIDSWKLPINLNTHEGFNLDHGHFNTATEIALGKWLADTYLSHYGFL 290
           +VTID+ KLPIN++THEGFN DHGHFNT T+I LGKWLADTYLSHYG L
Sbjct: 241 IVTIDALKLPINVDTHEGFNQDHGHFNTTTQITLGKWLADTYLSHYGHL 287

BLAST of Lag0037874 vs. NCBI nr
Match: XP_038886575.1 (probable carbohydrate esterase At4g34215 [Benincasa hispida])

HSP 1 Score: 464.5 bits (1194), Expect = 7.8e-127
Identity = 224/289 (77.51%), Postives = 252/289 (87.20%), Query Frame = 0

Query: 1   MALLRLSILLCMMLFGPSLSGATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPP 60
           MALL+L ILLC +LFG SLS A +PKNIFILAGQSNMAGRGGVEN++ GK L W+R +PP
Sbjct: 1   MALLKLLILLCTLLFGSSLSRAASPKNIFILAGQSNMAGRGGVENNQVGK-LEWNRLIPP 60

Query: 61  MCQPDPSILRLNPQLQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGL 120
            CQ D SILRLNP LQWE+AREP+H GID+ +KT GIGP M  A Q+ AKVG KAG+VGL
Sbjct: 61  ECQSDTSILRLNPALQWEMAREPLHEGIDI-NKTVGIGPGMPFAHQLLAKVGPKAGIVGL 120

Query: 121 VPCAKGGTLIKQWMKNPSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTA 180
           VPCAKGGT+I+QW+KNPSNP+ATFY++FIERIKASDKEGGVVRALFW  GESDAAM+DTA
Sbjct: 121 VPCAKGGTIIEQWIKNPSNPDATFYKSFIERIKASDKEGGVVRALFWFQGESDAAMNDTA 180

Query: 181 HKYKDNLKKFFTDVRNDIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPD 240
            +YKDNLK FFTD+RNDIKPRFLPII+VK  LYDF MKHDTHDLP VRAAQ+A+  ELPD
Sbjct: 181 SRYKDNLKNFFTDIRNDIKPRFLPIILVKIALYDFMMKHDTHDLPVVRAAQDAVSKELPD 240

Query: 241 VVTIDSWKLPINLNTHEGFNLDHGHFNTATEIALGKWLADTYLSHYGFL 290
           VVTID+ KLPIN++THEGFN DHGHFNT TEI LGKWLADTYLSHYG L
Sbjct: 241 VVTIDALKLPINVDTHEGFNQDHGHFNTTTEITLGKWLADTYLSHYGHL 287

BLAST of Lag0037874 vs. NCBI nr
Match: XP_022973316.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_022973317.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 463.4 bits (1191), Expect = 1.7e-126
Identity = 219/290 (75.52%), Postives = 252/290 (86.90%), Query Frame = 0

Query: 1   MALLRLSILLCMMLFGPSLSGATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPP 60
           M LL+LSIL+C +L  PSLSGAT+P NIFILAGQSNMAGRGGVE   +G+ LVWD  VP 
Sbjct: 1   MVLLKLSILMCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPTGE-LVWDGKVPS 60

Query: 61  MCQPDPSILRLNPQLQWEIAREPIHAGIDL-PDKTPGIGPAMAIARQIQAKVGQKAGVVG 120
            CQ DPSILR NP+ QWEIA EP+H GID+   KTPGIGP +  A Q++ K GQKAG+VG
Sbjct: 61  ECQSDPSILRFNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVG 120

Query: 121 LVPCAKGGTLIKQWMKNPSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDT 180
           LVPCA+GGTLI+QW+KNPSNP+ATFYQNFIERIK S+KEGGVVRALFW+ GESDAAM+DT
Sbjct: 121 LVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDT 180

Query: 181 AHKYKDNLKKFFTDVRNDIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELP 240
           A +YKDNLKKF TD+RNDIKPRFLP+IIVK  LYDFFMKHDTH+LPAVRAA++A+Q ELP
Sbjct: 181 AQRYKDNLKKFITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELP 240

Query: 241 DVVTIDSWKLPINLNTHEGFNLDHGHFNTATEIALGKWLADTYLSHYGFL 290
           D++TIDSW+LP+NL T EGF+ DHGHFNTATEIALGKWLADTYL+HYG L
Sbjct: 241 DIITIDSWELPMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHL 289

BLAST of Lag0037874 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 183.0 bits (463), Expect = 5.9e-45
Identity = 102/270 (37.78%), Postives = 147/270 (54.44%), Query Frame = 0

Query: 17  PSLSGATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQ 76
           P +     P  IFIL+GQSNMAGRGGV  D      VWD+ +PP C P+ SILRL+  L+
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 77  WEIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKN 136
           WE A EP+H  ID   K  G+GP MA A  ++ ++   + V+GLVPCA GGT IK+W + 
Sbjct: 73  WEEAHEPLHVDID-TGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER- 132

Query: 137 PSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRN 196
                +  Y+  ++R + S K GG ++A+ W+ GESD      A  Y +N+ +   ++R+
Sbjct: 133 ----GSHLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRH 192

Query: 197 DIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTH 256
           D+    LPII V       +       +  VR AQ  L  +L +VV +D+  LP+     
Sbjct: 193 DLNLPSLPIIQVAIASGGGY-------IDKVREAQLGL--KLSNVVCVDAKGLPL----- 252

Query: 257 EGFNLDHGHFNTATEIALGKWLADTYLSHY 287
                D+ H  T  ++ LG  LA  YLS++
Sbjct: 253 ---KSDNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of Lag0037874 vs. ExPASy TrEMBL
Match: A0A6J1KIR8 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111496116 PE=4 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 3.1e-129
Identity = 225/289 (77.85%), Postives = 254/289 (87.89%), Query Frame = 0

Query: 1   MALLRLSILLCMMLFGPSLSGATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPP 60
           M LL+LS LLCM+LF PSLS AT+P NIFILAGQSNMAGRGGVEN++ GK L WD  VP 
Sbjct: 1   MILLKLSTLLCMILFHPSLSWATSPTNIFILAGQSNMAGRGGVENNQKGK-LEWDGKVPL 60

Query: 61  MCQPDPSILRLNPQLQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGL 120
            CQ DPSILRLNP  QWEIA+EP+H GID+  KTPGIGP +  A Q +AK GQKAG+VGL
Sbjct: 61  ECQSDPSILRLNPARQWEIAQEPLHLGIDI-GKTPGIGPGIPFAHQFKAKAGQKAGIVGL 120

Query: 121 VPCAKGGTLIKQWMKNPSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTA 180
           VPCA+GGTLI+QW+KNPSNP+ATFYQNFIERIK S+KEGGVVRALFW+ GESDAAMSDTA
Sbjct: 121 VPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTA 180

Query: 181 HKYKDNLKKFFTDVRNDIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPD 240
           H+YKDNLKKF TD+RNDIKPRFLP+IIVK ++YDFFMKHDTHDLPAVRAA++A+Q ELPD
Sbjct: 181 HRYKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPD 240

Query: 241 VVTIDSWKLPINLNTHEGFNLDHGHFNTATEIALGKWLADTYLSHYGFL 290
           ++TIDSW+LPIN  T EGF LDHGHFNTATEIALGKWLADTYL+HY  L
Sbjct: 241 IITIDSWELPINFTTFEGFCLDHGHFNTATEIALGKWLADTYLAHYSHL 287

BLAST of Lag0037874 vs. ExPASy TrEMBL
Match: A0A6J1I774 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111471873 PE=4 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 8.4e-127
Identity = 219/290 (75.52%), Postives = 252/290 (86.90%), Query Frame = 0

Query: 1   MALLRLSILLCMMLFGPSLSGATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPP 60
           M LL+LSIL+C +L  PSLSGAT+P NIFILAGQSNMAGRGGVE   +G+ LVWD  VP 
Sbjct: 1   MVLLKLSILMCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPTGE-LVWDGKVPS 60

Query: 61  MCQPDPSILRLNPQLQWEIAREPIHAGIDL-PDKTPGIGPAMAIARQIQAKVGQKAGVVG 120
            CQ DPSILR NP+ QWEIA EP+H GID+   KTPGIGP +  A Q++ K GQKAG+VG
Sbjct: 61  ECQSDPSILRFNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVG 120

Query: 121 LVPCAKGGTLIKQWMKNPSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDT 180
           LVPCA+GGTLI+QW+KNPSNP+ATFYQNFIERIK S+KEGGVVRALFW+ GESDAAM+DT
Sbjct: 121 LVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDT 180

Query: 181 AHKYKDNLKKFFTDVRNDIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELP 240
           A +YKDNLKKF TD+RNDIKPRFLP+IIVK  LYDFFMKHDTH+LPAVRAA++A+Q ELP
Sbjct: 181 AQRYKDNLKKFITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELP 240

Query: 241 DVVTIDSWKLPINLNTHEGFNLDHGHFNTATEIALGKWLADTYLSHYGFL 290
           D++TIDSW+LP+NL T EGF+ DHGHFNTATEIALGKWLADTYL+HYG L
Sbjct: 241 DIITIDSWELPMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHL 289

BLAST of Lag0037874 vs. ExPASy TrEMBL
Match: A0A0A0LNC5 (SASA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G356040 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 5.4e-126
Identity = 221/289 (76.47%), Postives = 249/289 (86.16%), Query Frame = 0

Query: 1   MALLRLSILLCMMLFGPSLSGATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPP 60
           M LLRLSI+LC+ML+GPSLSGA +PKNIFILAGQSNMAGRGGVEN+  G NL WD  VPP
Sbjct: 1   MVLLRLSIILCVMLYGPSLSGAASPKNIFILAGQSNMAGRGGVENNAQG-NLQWDGLVPP 60

Query: 61  MCQPDPSILRLNPQLQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGL 120
            CQP PSILRLNP LQWEIAREP+H GID+  +TPGIGP +A A ++  K G  AG VGL
Sbjct: 61  ECQPQPSILRLNPGLQWEIAREPLHLGIDI-KRTPGIGPGIAFAHELLVKAGPNAGAVGL 120

Query: 121 VPCAKGGTLIKQWMKNPSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTA 180
           VPCA+GGTLI+QW+KNPSNP+ATFYQNFIERIKASDK+GGVVRALFW  GESDAAM+DTA
Sbjct: 121 VPCARGGTLIEQWIKNPSNPSATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMNDTA 180

Query: 181 HKYKDNLKKFFTDVRNDIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPD 240
            +YKDNLKKFFTD+R+DIKPRFLPII+VK  LYDFF +HDTH+LPAVR A+EA+  ELPD
Sbjct: 181 IRYKDNLKKFFTDIRDDIKPRFLPIIVVKIALYDFFRQHDTHNLPAVREAKEAVSKELPD 240

Query: 241 VVTIDSWKLPINLNTHEGFNLDHGHFNTATEIALGKWLADTYLSHYGFL 290
           VV IDS KLPIN  T+EG NLDHGHFNT TEI LGKWLA+TYLSH+G L
Sbjct: 241 VVAIDSLKLPINYTTNEGINLDHGHFNTTTEITLGKWLAETYLSHFGQL 287

BLAST of Lag0037874 vs. ExPASy TrEMBL
Match: A0A6J1GJF6 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454406 PE=4 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 6.0e-125
Identity = 218/289 (75.43%), Postives = 251/289 (86.85%), Query Frame = 0

Query: 1   MALLRLSILLCMMLFGPSLSGATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPP 60
           M LL+LSILLCM+LF PSLSGAT+P NIFILAGQSNMAGRGGVE  ++GK LVWD  VP 
Sbjct: 1   MVLLKLSILLCMILFNPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGK-LVWDGKVPL 60

Query: 61  MCQPDPSILRLNPQLQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGL 120
            CQ DPSILRLNP+ QWEIA EP+H GID+   TPGIGP +  A Q + K GQKAG+VGL
Sbjct: 61  ECQFDPSILRLNPERQWEIAHEPLHLGIDI-SHTPGIGPGIPFAHQFKEKAGQKAGIVGL 120

Query: 121 VPCAKGGTLIKQWMKNPSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTA 180
           VPCA+GGTLI+QW+KNPSNP+ATFYQNFIERIK S+KEGGVVRALFW+ GESDAAM+DTA
Sbjct: 121 VPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTA 180

Query: 181 HKYKDNLKKFFTDVRNDIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPD 240
            +YKDNLKKF TD+RNDIKPRFLP+IIVK +LYDFFMKHDTH+LPAVRAA++A+Q ELPD
Sbjct: 181 QRYKDNLKKFITDIRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPD 240

Query: 241 VVTIDSWKLPINLNTHEGFNLDHGHFNTATEIALGKWLADTYLSHYGFL 290
           ++TIDS +LP+N  T EGF+ DHGHFNT TEI LGKWLA+TYL+HYG L
Sbjct: 241 IITIDSRELPVNFTTFEGFSWDHGHFNTETEIVLGKWLANTYLAHYGHL 287

BLAST of Lag0037874 vs. ExPASy TrEMBL
Match: A0A6J1BQ38 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111004778 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 7.8e-125
Identity = 223/289 (77.16%), Postives = 254/289 (87.89%), Query Frame = 0

Query: 1   MALLRLSILLCMMLFGPSLSGATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPP 60
           MALLRLSI+LCMMLFGPSLSGAT+PKNIFILAGQSNMAGRGGVE +++G +L WD YVPP
Sbjct: 1   MALLRLSIMLCMMLFGPSLSGATSPKNIFILAGQSNMAGRGGVEKNRTG-DLEWDGYVPP 60

Query: 61  MCQPDPSILRLNPQLQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGL 120
             QPDPSILRLNP+ QWE+AREP+H GID+  KT G+GPA+A A Q+QAK G K G VGL
Sbjct: 61  ESQPDPSILRLNPERQWEVAREPVHRGIDI-GKTVGVGPAIAFAHQLQAKGGSKVGSVGL 120

Query: 121 VPCAKGGTLIKQWMKNPSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTA 180
           VPCA+GGTLI+QW+KNPSNPNATFY+NFIERI+ASD+EGGVVRALFW  GESDAA SDTA
Sbjct: 121 VPCARGGTLIEQWVKNPSNPNATFYKNFIERIQASDREGGVVRALFWLQGESDAASSDTA 180

Query: 181 HKYKDNLKKFFTDVRNDIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPD 240
            +YK+NLKKFFTD+RNDIKPR LPII+VK  +YD FMKHDTHDLPAVRAA++A+Q ELP+
Sbjct: 181 ERYKNNLKKFFTDIRNDIKPRVLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPN 240

Query: 241 VVTIDSWKLPINLNTHEGFNLDHGHFNTATEIALGKWLADTYLSHYGFL 290
           VVTID+ KL +N  T EGFNLD GHFN  TEIALGKWLADTYLS+YG L
Sbjct: 241 VVTIDALKL-VNTTTAEGFNLDRGHFNIKTEIALGKWLADTYLSNYGHL 286

BLAST of Lag0037874 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 183.7 bits (465), Expect = 2.5e-46
Identity = 112/273 (41.03%), Postives = 157/273 (57.51%), Query Frame = 0

Query: 17  PSLSGATTPKN--IFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQ 76
           P L   T  +N  IFILAGQSNMAGRGGV ND +    VWD  +PP C+ +PSILRL  +
Sbjct: 18  PHLQSQTITRNISIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSK 77

Query: 77  LQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWM 136
           L+W+ A+EP+H  ID+ +KT G+GP M  A ++  + GQ    VGLVPC+ GGT + QW 
Sbjct: 78  LEWKEAKEPLHVDIDI-NKTNGVGPGMPFANRVVNRFGQ----VGLVPCSIGGTKLSQWQ 137

Query: 137 KNPSNPNATFYQNFIERIKA--SDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFT 196
           K         Y+  ++R KA  +   GG  RA+ W+ GESD      A  YK  L KFF+
Sbjct: 138 K-----GEFLYEETVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFS 197

Query: 197 DVRNDIKPRFLPII-IVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPI 256
           D+RND++   LPII +  AT    +       L AVR AQ  L+ +L +V  +D+  LP+
Sbjct: 198 DLRNDLQHPNLPIIQVALATGAGPY-------LDAVRKAQ--LKTDLENVYCVDARGLPL 257

Query: 257 NLNTHEGFNLDHGHFNTATEIALGKWLADTYLS 285
                     D  H  T++++ LG  +A+++L+
Sbjct: 258 --------EPDGLHLTTSSQVQLGHMIAESFLA 263

BLAST of Lag0037874 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 183.0 bits (463), Expect = 4.2e-46
Identity = 102/270 (37.78%), Postives = 147/270 (54.44%), Query Frame = 0

Query: 17  PSLSGATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQ 76
           P +     P  IFIL+GQSNMAGRGGV  D      VWD+ +PP C P+ SILRL+  L+
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 77  WEIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKN 136
           WE A EP+H  ID   K  G+GP MA A  ++ ++   + V+GLVPCA GGT IK+W + 
Sbjct: 73  WEEAHEPLHVDID-TGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER- 132

Query: 137 PSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRN 196
                +  Y+  ++R + S K GG ++A+ W+ GESD      A  Y +N+ +   ++R+
Sbjct: 133 ----GSHLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRH 192

Query: 197 DIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTH 256
           D+    LPII V       +       +  VR AQ  L  +L +VV +D+  LP+     
Sbjct: 193 DLNLPSLPIIQVAIASGGGY-------IDKVREAQLGL--KLSNVVCVDAKGLPL----- 252

Query: 257 EGFNLDHGHFNTATEIALGKWLADTYLSHY 287
                D+ H  T  ++ LG  LA  YLS++
Sbjct: 253 ---KSDNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of Lag0037874 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 183.0 bits (463), Expect = 4.2e-46
Identity = 102/270 (37.78%), Postives = 147/270 (54.44%), Query Frame = 0

Query: 17  PSLSGATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQ 76
           P +     P  IFIL+GQSNMAGRGGV  D      VWD+ +PP C P+ SILRL+  L+
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 77  WEIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKN 136
           WE A EP+H  ID   K  G+GP MA A  ++ ++   + V+GLVPCA GGT IK+W + 
Sbjct: 73  WEEAHEPLHVDID-TGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER- 132

Query: 137 PSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRN 196
                +  Y+  ++R + S K GG ++A+ W+ GESD      A  Y +N+ +   ++R+
Sbjct: 133 ----GSHLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRH 192

Query: 197 DIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTH 256
           D+    LPII V       +       +  VR AQ  L  +L +VV +D+  LP+     
Sbjct: 193 DLNLPSLPIIQVAIASGGGY-------IDKVREAQLGL--KLSNVVCVDAKGLPL----- 252

Query: 257 EGFNLDHGHFNTATEIALGKWLADTYLSHY 287
                D+ H  T  ++ LG  LA  YLS++
Sbjct: 253 ---KSDNLHLTTEAQVQLGLSLAQAYLSNF 259