Lag0037864 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0037864
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	PMEI domain-containing protein
Location	chr2: 9961361 .. 9961885 (+)
RNA-Seq Expression	Lag0037864
Synteny	Lag0037864

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATAATCTCCTCCATTCCCTGCTTTCATCTCTCGTCCTCTTCGCGCTCTTCTCTCCGGCGAAACCAGACGCCGCCTTGATCGGAAACCTCTGCAGCAAGACCGAGCAGCCGGTCATTTGTTCCGATTGCCTGAATTCTGACTCCGGAAGCCAGACCGCCGACGCCCGTGGACTCGCAGTCATCGCCGTTGGATGCGCCGAGAGAAATACCAAATTAATGGCGCAGAGGATCGGCGACCTTATTCGAAGGACACCGGATTCCCCACTGAAGACGATTTTGAACGATTGCTGGTTTTCGACGGGGTATGCCGCCGGCAACTTTCCGGGAATCGCACGCTCCATCGCGGCGGGAGACTACGCCGGCGGGAGGAACGTCTTGGAGATTACGATTAGGAATGTGAATAGCTGCTTGGCTGAGTTCAATAGAAACCCTAGCATTCCGGTGCCGTCGGAGGTTTTGGCGGGGACGGTGGCGGCCGTCCAAAGCTGCCGGATTGTTTCTGAGATTTTGAGTAATATTTGA

mRNA sequence

ATGAATAATCTCCTCCATTCCCTGCTTTCATCTCTCGTCCTCTTCGCGCTCTTCTCTCCGGCGAAACCAGACGCCGCCTTGATCGGAAACCTCTGCAGCAAGACCGAGCAGCCGGTCATTTGTTCCGATTGCCTGAATTCTGACTCCGGAAGCCAGACCGCCGACGCCCGTGGACTCGCAGTCATCGCCGTTGGATGCGCCGAGAGAAATACCAAATTAATGGCGCAGAGGATCGGCGACCTTATTCGAAGGACACCGGATTCCCCACTGAAGACGATTTTGAACGATTGCTGGTTTTCGACGGGGTATGCCGCCGGCAACTTTCCGGGAATCGCACGCTCCATCGCGGCGGGAGACTACGCCGGCGGGAGGAACGTCTTGGAGATTACGATTAGGAATGTGAATAGCTGCTTGGCTGAGTTCAATAGAAACCCTAGCATTCCGGTGCCGTCGGAGGTTTTGGCGGGGACGGTGGCGGCCGTCCAAAGCTGCCGGATTGTTTCTGAGATTTTGAGTAATATTTGA

Coding sequence (CDS)

ATGAATAATCTCCTCCATTCCCTGCTTTCATCTCTCGTCCTCTTCGCGCTCTTCTCTCCGGCGAAACCAGACGCCGCCTTGATCGGAAACCTCTGCAGCAAGACCGAGCAGCCGGTCATTTGTTCCGATTGCCTGAATTCTGACTCCGGAAGCCAGACCGCCGACGCCCGTGGACTCGCAGTCATCGCCGTTGGATGCGCCGAGAGAAATACCAAATTAATGGCGCAGAGGATCGGCGACCTTATTCGAAGGACACCGGATTCCCCACTGAAGACGATTTTGAACGATTGCTGGTTTTCGACGGGGTATGCCGCCGGCAACTTTCCGGGAATCGCACGCTCCATCGCGGCGGGAGACTACGCCGGCGGGAGGAACGTCTTGGAGATTACGATTAGGAATGTGAATAGCTGCTTGGCTGAGTTCAATAGAAACCCTAGCATTCCGGTGCCGTCGGAGGTTTTGGCGGGGACGGTGGCGGCCGTCCAAAGCTGCCGGATTGTTTCTGAGATTTTGAGTAATATTTGA

Protein sequence

MNNLLHSLLSSLVLFALFSPAKPDAALIGNLCSKTEQPVICSDCLNSDSGSQTADARGLAVIAVGCAERNTKLMAQRIGDLIRRTPDSPLKTILNDCWFSTGYAAGNFPGIARSIAAGDYAGGRNVLEITIRNVNSCLAEFNRNPSIPVPSEVLAGTVAAVQSCRIVSEILSNI

Homology

[Close]

BLAST of Lag0037864 vs. NCBI nr
Match: XP_038896712.1 (pectinesterase inhibitor-like [Benincasa hispida])

HSP 1 Score: 284.6 bits (727), Expect = 5.6e-73
Identity = 140/172 (81.40%), Postives = 153/172 (88.95%), Query Frame = 0


Query: 3   NLLHSLLSSLVLFALFSPAKPDAALIGNLCSKTEQPVICSDCLNSDSGSQTADARGLAVI 62
           NLL S+L  +VLFALFSPAKPDAALI NLC KTEQP ICSDCLNS SGSQTAD  GLA+I
Sbjct: 2   NLLSSILPIIVLFALFSPAKPDAALIANLCCKTEQPKICSDCLNSGSGSQTADGLGLALI 61

Query: 63  AVGCAERNTKLMAQRIGDLIRRTPDSPLKTILNDCWFSTGYAAGNFPGIARSIAAGDYAG 122
           A+GCAE+NTKLMAQR+G L+R TPDS LKT+LNDCW STG AAGNFPGIARS+AAGDYAG
Sbjct: 62  AIGCAEKNTKLMAQRLGGLVRSTPDSTLKTLLNDCWLSTGSAAGNFPGIARSVAAGDYAG 121

Query: 123 GRNVLEITIRNVNSCLAEFNRNPSIPVPSEVLAGTVAAVQSCRIVSEILSNI 175
           GRNVLEITIRN NSCL  FN+NPS+PVPSEVLAGTVAA Q CRI+SEIL+NI
Sbjct: 122 GRNVLEITIRNANSCLDNFNKNPSVPVPSEVLAGTVAAAQDCRIISEILNNI 173

[Close]

BLAST of Lag0037864 vs. NCBI nr
Match: XP_008463801.1 (PREDICTED: pectinesterase inhibitor-like [Cucumis melo] >KAA0066803.1 pectinesterase inhibitor-like [Cucumis melo var. makuwa] >TYK27950.1 pectinesterase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 272.7 bits (696), Expect = 2.2e-69
Identity = 133/172 (77.33%), Postives = 151/172 (87.79%), Query Frame = 0


Query: 3   NLLHSLLSSLVLFALFSPAKPDAALIGNLCSKTEQPVICSDCLNSDSGSQTADARGLAVI 62
           NLL+S+L   +LFALFSPAKPDAA IGNLCSKTEQP ICSDCLNS  GSQ+A  RGLA+I
Sbjct: 2   NLLNSILLISLLFALFSPAKPDAAYIGNLCSKTEQPKICSDCLNSGRGSQSASGRGLALI 61

Query: 63  AVGCAERNTKLMAQRIGDLIRRTPDSPLKTILNDCWFSTGYAAGNFPGIARSIAAGDYAG 122
           AVGCAERNTKLMAQR+G+L+R TP+S LK +LN+CW STGYAAGNFPGIARS+AAGDYA 
Sbjct: 62  AVGCAERNTKLMAQRVGNLVRSTPESTLKMLLNECWMSTGYAAGNFPGIARSVAAGDYAS 121

Query: 123 GRNVLEITIRNVNSCLAEFNRNPSIPVPSEVLAGTVAAVQSCRIVSEILSNI 175
           G+NVLEITIRNVN CL  F +NP+IPVPSEVLAGTVA  Q CRIV++IL+NI
Sbjct: 122 GKNVLEITIRNVNMCLVNFKKNPNIPVPSEVLAGTVAVTQDCRIVTQILNNI 173

[Close]

BLAST of Lag0037864 vs. NCBI nr
Match: KAG6607897.1 (hypothetical protein SDJN03_01239, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 263.1 bits (671), Expect = 1.7e-66
Identity = 129/163 (79.14%), Postives = 145/163 (88.96%), Query Frame = 0


Query: 12  LVLFALFSPAKPDAALIGNLCSKTEQPVICSDCLNSDSGSQTADARGLAVIAVGCAERNT 71
           L LFALFSPAKPDAALI NLC +TEQPVICS CLNSDS S+TA+ RGLAVIAVGCAERNT
Sbjct: 7   LALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVGCAERNT 66

Query: 72  KLMAQRIGDLIRRTPDSPLKTILNDCWFSTGYAAGNFPGIARSIAAGDYAGGRNVLEITI 131
           + MAQRIGD+ RRTP+SP+KT+LNDCWFSTGYAAGNFPGI  ++  GDYA  RN +EI+I
Sbjct: 67  RAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARNGVEISI 126

Query: 132 RNVNSCLAEFNRNPSIPVPSEVLAGTVAAVQSCRIVSEILSNI 175
           RNVNSCLA F++NPS+PVPSEVLAGTVAAVQSCRIV  IL+NI
Sbjct: 127 RNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 169

[Close]

BLAST of Lag0037864 vs. NCBI nr
Match: XP_022940751.1 (uncharacterized protein LOC111446254 [Cucurbita moschata] >KAG7037425.1 hypothetical protein SDJN02_01051, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 263.1 bits (671), Expect = 1.7e-66
Identity = 129/163 (79.14%), Postives = 145/163 (88.96%), Query Frame = 0


Query: 12  LVLFALFSPAKPDAALIGNLCSKTEQPVICSDCLNSDSGSQTADARGLAVIAVGCAERNT 71
           L LFALFSPAKPDAALI NLC +TEQPVICS CLNSDS S+TA+ RGLAVIAVGCAERNT
Sbjct: 13  LALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVGCAERNT 72

Query: 72  KLMAQRIGDLIRRTPDSPLKTILNDCWFSTGYAAGNFPGIARSIAAGDYAGGRNVLEITI 131
           + MAQRIGD+ RRTP+SP+KT+LNDCWFSTGYAAGNFPGI  ++  GDYA  RN +EI+I
Sbjct: 73  RAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRSAVQGGDYARARNGVEISI 132

Query: 132 RNVNSCLAEFNRNPSIPVPSEVLAGTVAAVQSCRIVSEILSNI 175
           RNVNSCLA F++NPS+PVPSEVLAGTVAAVQSCRIV  IL+NI
Sbjct: 133 RNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNNI 175

[Close]

BLAST of Lag0037864 vs. NCBI nr
Match: XP_023523451.1 (uncharacterized protein LOC111787665 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 261.5 bits (667), Expect = 5.0e-66
Identity = 128/163 (78.53%), Postives = 145/163 (88.96%), Query Frame = 0


Query: 12  LVLFALFSPAKPDAALIGNLCSKTEQPVICSDCLNSDSGSQTADARGLAVIAVGCAERNT 71
           L LFALFSPAKPDAALI NLC +TEQPVICS CLNSDS S+TA+ RGLAVIAVGCAERNT
Sbjct: 39  LALFALFSPAKPDAALIRNLCGRTEQPVICSACLNSDSASRTANGRGLAVIAVGCAERNT 98

Query: 72  KLMAQRIGDLIRRTPDSPLKTILNDCWFSTGYAAGNFPGIARSIAAGDYAGGRNVLEITI 131
           + MAQRIGD+ RRTP+SP+KT+LNDCWFSTGYAAGNFPGI  ++  GDYA  RN +EI+I
Sbjct: 99  RAMAQRIGDISRRTPNSPVKTLLNDCWFSTGYAAGNFPGIRNAVQGGDYARARNGVEISI 158

Query: 132 RNVNSCLAEFNRNPSIPVPSEVLAGTVAAVQSCRIVSEILSNI 175
           RNVNSCLA F++NPS+PVPSEVLAGTVAAVQSCRIV  IL++I
Sbjct: 159 RNVNSCLAGFDKNPSVPVPSEVLAGTVAAVQSCRIVLGILNHI 201

[Close]

BLAST of Lag0037864 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 1.6e-06
Identity = 41/149 (27.52%), Postives = 61/149 (40.94%), Query Frame = 0


Query: 7   SLLSSLVLFALF-SP------AKPDAALIGNLCSKTEQPVICSDCLNSDSGSQTADARGL 66
           SL  SL+L  LF SP       K +  LI  +C KT  P +C   L SD  S + D +GL
Sbjct: 9   SLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLKGL 68

Query: 67  AVIAVGCAERNTKLMAQRIGDLIRRTPDSPLKTILNDCWFSTGYAAGNFPGIARSIAAGD 126
              ++  A+ + K  ++ I  L  +  D  LK     C  +   A  +     + + +GD
Sbjct: 69  GQFSIDIAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQAKQFLTSGD 128

Query: 127 YAGGRNVLEITIRNVNSCLAEFNRNPSIP 149
           Y               +C   F   P+IP
Sbjct: 129 YNSLNIYASAAFDGAGTCEDSFEGPPNIP 157

The following BLAST results are available for this feature:

BLAST of Lag0037864 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896712.1	5.6e-73	81.40	pectinesterase inhibitor-like [Benincasa hispida]	[more]
XP_008463801.1	2.2e-69	77.33	PREDICTED: pectinesterase inhibitor-like [Cucumis melo] >KAA0066803.1 pectineste...	[more]
KAG6607897.1	1.7e-66	79.14	hypothetical protein SDJN03_01239, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022940751.1	1.7e-66	79.14	uncharacterized protein LOC111446254 [Cucurbita moschata] >KAG7037425.1 hypothet...	[more]
XP_023523451.1	5.0e-66	78.53	uncharacterized protein LOC111787665 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0037864 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83326	1.6e-06	27.52	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 23..170 e-value: 2.3E-9 score: 47.1
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 27..156 e-value: 2.0E-12 score: 47.7
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 2..151 e-value: 3.1E-22 score: 76.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 24..174 e-value: 1.0E-23 score: 86.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 26..172
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 12..174
None	No IPR available	PANTHER	PTHR31080:SF83	CELL WALL / VACUOLAR INHIBITOR OF FRUCTOSIDASE 2-LIKE	coord: 12..174
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 24..174 e-value: 3.24657E-31 score: 108.278

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0037864.1	Lag0037864.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity