Lag0037862 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0037862
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	E3 SUMO-protein ligase MMS21
Location	chr2: 9952912 .. 9955602 (-)
RNA-Seq Expression	Lag0037862
Synteny	Lag0037862

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCACTTCCAATTCCCGTTCAAGTGGCGTTTCCGATAGAATCAAATCTGCTGCCACTATTATGCACTCCGAGAATCAATCCCTTCTTGCCGTAACTTCCCTTAATACTACCCTCTAGTCTACTTCTATGACTGCTTTTAATTTCTCGTCGTTTTCGTTCTAGTTCTTGCGTTTTTCTGATTCATAGAGGGATTTTTGTTCTGGGAAATCCGGCTCTGGAAAGAAAATTGACGTCGCTGTTGCTAAATCAGTGTCGGTTTCAGCTTTAAGATCTCGATTTTTTTTTTTTTTTGTATAGCGAGTCAGTTCTTATGCTGCATTCCCTTCACTCTCCGCTGTGAACTGGGTTTTCTTGATTTCTGAAAGCTAAAACGTCAGGGGCTTGAGTGATTTGTTAGGAATTTGATTAATGATGATGGGTTGTCTGTTTGTTTTTGTGATTTATTTGATTTTACTGTCTTAATTTCAGGAATTACGGAAGTCGCTGATTATGATGAAGGAAATTGGGGTGGATTTGGAGAAGGACAACCAGTCGAGGATGGTAATTTTATTGATTGATAATGAACCTTAATTTTGAGCATAAAAAATGATGGTGGTTTGTTACTTTGGAAGATAATGTTTTCAAAACAAGGTACTTTTGTAAAATGTTAAGAAGCAAGGACAACACAAATTTCAAAGATTTTCTCTTGGACTCTCATCACAAGGCTTTATTTTAACATCTTTGGAGTTTCTTTCTTAGTTAATTGAAAATAAAATGTGAGTGAAGGAAAACAAGATGTATGTGCAGCGTATTGTGTACAACCTGCTTAGGAACTTTCACTATAAACCAGCCACTTTGTCAAACCGTTCAAAATTCAAATGTTTGAAATTCTTAATTCTATTCAGTGATTTTTCTTGATTAGGTTTTTGGTTTTGGATAGACTTTCTATGGATGCTACGATATGATCGAGATTTATTTGAATTGATTCCTTGCTTAAAAATTATGATCTTGGTTTCACTTGGTGTACATTAACTAATTACTATACCAAATATACTTTTTGGTGGATTTTCTGCTTCTGTCTATGAGATCCAATCTAAAGATGATTGAGAATCATTGTTGTTTGGAACTCAACCAATAATTTACTTGGTATTTGAACATTATCTTAGGTCAAGGAGCTTGAAAATTCTGTTGTTGAGCTGCTGGGTACTTATGAATCCTGTAACAACTTTTCATCTGCAATTCAGTCGGTTGGAAACACATATGAACCAAAAGAAGAGGTGCTTAAATCTCTTTCCATGTTGAAATTTCCACTACACATTCTTTTATTAATTTTCTTTGTGCTGGTTCCTTCTTTTTCACACCTAATATTTATGTTGATATTGTAGTTAACAGATTTTGAGAAACTACTCGACGACGAAGTTGCAAAAATCAGTGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGGAATTTTAGAGAAGCTATTTGGGTATGCATATTCATCTTCTTTTATACGCATTAGGAAGTGAAAGTATTCCAGTTTTATGACCTGATTCTAGGAAACTTTGGAATGCGTTGCCTCACTTCTTTGACTTTCTATTTGTCGGCCTTTTGCTTTGATTATACATGGCAGCAACAAGAGAATCCTCAAATCTGTTCAAATATTTATCCTGAAACTGTGAAAATGACCAACAGAAAGTACATGAGTAGTAAACAAGAGAAACTAGTTTACCTGTTTGCAATTTACAAATGTTTGGAAAATATTTTATTGTTCTTCCAATTGTAGATTTTATTATTTCCAATTCCAGTGCTTTAGCCTATCTTCCGGTATTCCTGCAGAATGTTCATCATGCAGGACAGCCGATGCCAGGTGAGGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATCTATTGAATGTCACTTGCCCATTGAGTGGAAAGCCTGTCACTGAATTAGCGGAACCGGTTCGCAGGTATGTTGCAACTAAAGATATTTTGATGAAGGATAAAAAATTGGAATTATTTCTCTTACTGTTGAAAAAGAAGTCTGCTCATCCATTCGCTCTGTAATAACAGCATGAATGTTTCAAGGGAATGGCTACATGCTTTATTTTCTCTAGTTACATAAGCCTGAAAATGGAATGAGATATTCTCTCACTGGGATGAGTTTAGAAATTCTTCTTGTGTAGGACCTCTTGTTTTTCCATGGTTTTTGTTCTTCGAGTTCAACCACGTGTAGGGATGGAGATTCAAACTTCTGACCTTTTAGTCGAGGATATAATATCTTAACTCGTTTAGCTATGTTCAGATTAGTGCAATGACTCAACCATCATACATTTAATCACTTTTATTAAGTCTCTTTTTGTTTCTACATTCTGAAATTTGTAGTATGGAATGCAAGCACGTATACGAAAAGGCGGTCATAATGCAGTACCTAAAATCGAAGAAATCTCGAGCTCAATGCCCTGTTGCTGGTATTCAGATCTTCTGCATTACATGTACAACATACAATATGTTATTGTATTAGATTGCTCATCTGACTTAATGTCTTTGTCCTTGTAGCCTGTCCCAGGATGTTGCAGCCCGAAAAGGTCATTTCTGATCCATTCTTACAGATTGAAATTGATGAACTGCGAAAAGTGTCTAGGCATTCTGTGAGGATACAGGATTTCACAGAGCTTGATGCAGATTAG

mRNA sequence

ATGGCTTCCACTTCCAATTCCCGTTCAAGTGGCGTTTCCGATAGAATCAAATCTGCTGCCACTATTATGCACTCCGAGAATCAATCCCTTCTTGCCGAATTACGGAAGTCGCTGATTATGATGAAGGAAATTGGGGTGGATTTGGAGAAGGACAACCAGTCGAGGATGGTCAAGGAGCTTGAAAATTCTGTTGTTGAGCTGCTGGGTACTTATGAATCCTGTAACAACTTTTCATCTGCAATTCAGTCGGTTGGAAACACATATGAACCAAAAGAAGAGTTAACAGATTTTGAGAAACTACTCGACGACGAAGTTGCAAAAATCAGTGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGGAATTTTAGAGAAGCTATTTGGAATGTTCATCATGCAGGACAGCCGATGCCAGGTGAGGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATCTATTGAATGTCACTTGCCCATTGAGTGGAAAGCCTGTCACTGAATTAGCGGAACCGGTTCGCAGTATGGAATGCAAGCACGTATACGAAAAGGCGGTCATAATGCAGTACCTAAAATCGAAGAAATCTCGAGCTCAATGCCCTGTTGCTGCCTGTCCCAGGATGTTGCAGCCCGAAAAGGTCATTTCTGATCCATTCTTACAGATTGAAATTGATGAACTGCGAAAAGTGTCTAGGCATTCTGTGAGGATACAGGATTTCACAGAGCTTGATGCAGATTAG

Coding sequence (CDS)

ATGGCTTCCACTTCCAATTCCCGTTCAAGTGGCGTTTCCGATAGAATCAAATCTGCTGCCACTATTATGCACTCCGAGAATCAATCCCTTCTTGCCGAATTACGGAAGTCGCTGATTATGATGAAGGAAATTGGGGTGGATTTGGAGAAGGACAACCAGTCGAGGATGGTCAAGGAGCTTGAAAATTCTGTTGTTGAGCTGCTGGGTACTTATGAATCCTGTAACAACTTTTCATCTGCAATTCAGTCGGTTGGAAACACATATGAACCAAAAGAAGAGTTAACAGATTTTGAGAAACTACTCGACGACGAAGTTGCAAAAATCAGTGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGGAATTTTAGAGAAGCTATTTGGAATGTTCATCATGCAGGACAGCCGATGCCAGGTGAGGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATCTATTGAATGTCACTTGCCCATTGAGTGGAAAGCCTGTCACTGAATTAGCGGAACCGGTTCGCAGTATGGAATGCAAGCACGTATACGAAAAGGCGGTCATAATGCAGTACCTAAAATCGAAGAAATCTCGAGCTCAATGCCCTGTTGCTGCCTGTCCCAGGATGTTGCAGCCCGAAAAGGTCATTTCTGATCCATTCTTACAGATTGAAATTGATGAACTGCGAAAAGTGTCTAGGCATTCTGTGAGGATACAGGATTTCACAGAGCTTGATGCAGATTAG

Protein sequence

MASTSNSRSSGVSDRIKSAATIMHSENQSLLAELRKSLIMMKEIGVDLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVRIQDFTELDAD

Homology

[Close]

BLAST of Lag0037862 vs. NCBI nr
Match: XP_022941288.1 (E3 SUMO-protein ligase MMS21 [Cucurbita moschata])

HSP 1 Score: 443.7 bits (1140), Expect = 1.0e-120
Identity = 226/248 (91.13%), Postives = 239/248 (96.37%), Query Frame = 0


Query: 1   MASTSNSRSSGVSDRIKSAATIMHSENQSLLAELRKSLIMMKEIGVDLEKDNQSRMVKEL 60
           MASTSNS S+GVS+RIKSAATIM+SENQSLLAELRKSLIMMKEIGV+LE+DNQSRMVKEL
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVELERDNQSRMVKEL 60

Query: 61  ENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQNHS 120
           ENSVVELLGTYE C+NFSSAIQSVGN YEP+EELTDFEKLLDDEVAK+SENSSSNLQNHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSMEC 180
           IIR FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELAEPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVRIQ 240
           KHVYEK  IMQYLKSK+SRAQCPVAACP+MLQPEKVISDPFL IEIDELRK S+HSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAD 249
           DFTE+DAD
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Lag0037862 vs. NCBI nr
Match: XP_023525588.1 (E3 SUMO-protein ligase MMS21 [Cucurbita pepo subsp. pepo] >KAG7037426.1 E3 SUMO-protein ligase MMS21 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 443.4 bits (1139), Expect = 1.3e-120
Identity = 226/248 (91.13%), Postives = 239/248 (96.37%), Query Frame = 0


Query: 1   MASTSNSRSSGVSDRIKSAATIMHSENQSLLAELRKSLIMMKEIGVDLEKDNQSRMVKEL 60
           MASTSNS S+GVS+RIKSAATIM+SENQSLLAELRKSLIMMKEIGVDLE+DNQSRMVKEL
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60

Query: 61  ENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQNHS 120
           ENSVVELLGTYE C+NFSSAIQSVGN YEP+EELTDFEKLLD+EVAK+SENSSSNLQNHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS 120

Query: 121 IIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSMEC 180
           IIR FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELAEPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVRIQ 240
           KHVYEK  IMQYLKSK+SRAQCPVAACP+MLQPEKVISDPFL IEIDELRK S+HSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAD 249
           DFTE+DAD
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Lag0037862 vs. NCBI nr
Match: KAG6607898.1 (E3 SUMO-protein ligase MMS21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 441.4 bits (1134), Expect = 5.1e-120
Identity = 225/248 (90.73%), Postives = 238/248 (95.97%), Query Frame = 0


Query: 1   MASTSNSRSSGVSDRIKSAATIMHSENQSLLAELRKSLIMMKEIGVDLEKDNQSRMVKEL 60
           MASTSNS S+GVS+RIKSAATIM+SENQSLLAELRKSLIMMKEIGVDLE+DNQSRMVKEL
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60

Query: 61  ENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQNHS 120
           ENSVVELLGTYE C+NFSSAIQSVGN YEP+EELTDFEKLLD+EVAK+SENSSSNL NHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLHNHS 120

Query: 121 IIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSMEC 180
           IIR FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELAEPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVRIQ 240
           KHVYEK  IMQYLKSK+SRAQCPVAACP+MLQPEKVISDPFL IEIDELRK S+HSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAD 249
           DFTE+DAD
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Lag0037862 vs. NCBI nr
Match: XP_022981951.1 (E3 SUMO-protein ligase MMS21 [Cucurbita maxima])

HSP 1 Score: 436.0 bits (1120), Expect = 2.1e-118
Identity = 225/250 (90.00%), Postives = 238/250 (95.20%), Query Frame = 0


Query: 1   MAST--SNSRSSGVSDRIKSAATIMHSENQSLLAELRKSLIMMKEIGVDLEKDNQSRMVK 60
           MAST  SNS S+GVS+RIKSAATIM+SENQSLLAELRKSLIMMKEIGVDLE+DNQSRMVK
Sbjct: 1   MASTSNSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVK 60

Query: 61  ELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQN 120
           ELENSVVELL TYE C+NFSSAIQSVGN YEP+EELTDFEKLLD+EVAK+SENSSSNLQN
Sbjct: 61  ELENSVVELLSTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQN 120

Query: 121 HSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSM 180
           HSIIR FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELAEPVRS 
Sbjct: 121 HSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRST 180

Query: 181 ECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVR 240
           ECKHVYEK  IMQYLKSK+SRAQCPVAACP+MLQPEKVISDPFL IEIDELRK S+HSVR
Sbjct: 181 ECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR 240

Query: 241 IQDFTELDAD 249
           IQDFTE+DAD
Sbjct: 241 IQDFTEVDAD 250

[Close]

BLAST of Lag0037862 vs. NCBI nr
Match: XP_022139610.1 (E3 SUMO-protein ligase MMS21 isoform X1 [Momordica charantia])

HSP 1 Score: 427.9 bits (1099), Expect = 5.8e-116
Identity = 217/248 (87.50%), Postives = 234/248 (94.35%), Query Frame = 0


Query: 1   MASTSNSRSSGVSDRIKSAATIMHSENQSLLAELRKSLIMMKEIGVDLEKDNQSRMVKEL 60
           MASTS++RSS VS RIKSAAT+MHSENQSLLAE+RK LIMMKEIG+DLE+DNQS MVK+L
Sbjct: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60

Query: 61  ENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQNHS 120
           EN+VVELL TYE+C+NFSSAIQSVGN YEP+EELTDFEKLLDDEVAK+SENSSSNLQNHS
Sbjct: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSMEC 180
           IIR FREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELA+PVRSMEC
Sbjct: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180

Query: 181 KHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVRIQ 240
           KHVYEK  +MQYL+SK SRAQCPVAACP+MLQPEKV  DPFL IEIDELRK SRHSVRIQ
Sbjct: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240

Query: 241 DFTELDAD 249
           DFTELDA+
Sbjct: 241 DFTELDAE 248

[Close]

BLAST of Lag0037862 vs. ExPASy Swiss-Prot
Match: Q8GYH7 (E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana OX=3702 GN=MMS21 PE=1 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 6.3e-73
Identity = 142/243 (58.44%), Postives = 185/243 (76.13%), Query Frame = 0


Query: 5   SNSRSSGVSDRIKSAATIMHSENQSLLAELRKSLIMMKEIGVDLEKDNQSRMVKELENSV 64
           S S S GV+ RI++A+ ++ S+N S LA++RK++ MMK I V LEK+NQ+  VK+LENSV
Sbjct: 3   SASSSDGVAGRIQNASLVLVSDNSSTLADIRKAVAMMKNIAVQLEKENQTDKVKDLENSV 62

Query: 65  VELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQNHSIIRN 124
            ELL  +  CN+ S+AIQSV N Y+P E+LTDF+KLLDDE  K+    SS  QN  ++R 
Sbjct: 63  AELLDLHSDCNHRSTAIQSVANRYQPVEQLTDFKKLLDDEFTKLKATPSSVPQNDHLMRQ 122

Query: 125 FREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSMECKHVY 184
           FREA+WNVHHAG+PMPG++ EDIVMTSTQC LLN+TCPLSGKPVTELA+PVRSM+C+HVY
Sbjct: 123 FREAVWNVHHAGEPMPGDDDEDIVMTSTQCPLLNMTCPLSGKPVTELADPVRSMDCRHVY 182

Query: 185 EKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVR---IQD 244
           EK+VI+ Y+ +  + A CPVA C   LQ  KVI D  L+ EI+E+R +++ S R   I+D
Sbjct: 183 EKSVILHYIVNNPN-ANCPVAGCRGKLQNSKVICDAMLKFEIEEMRSLNKQSNRAEVIED 242

[Close]

BLAST of Lag0037862 vs. ExPASy TrEMBL
Match: A0A6J1FRP6 (E3 SUMO-protein ligase MMS21 OS=Cucurbita moschata OX=3662 GN=LOC111446631 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 4.9e-121
Identity = 226/248 (91.13%), Postives = 239/248 (96.37%), Query Frame = 0


Query: 1   MASTSNSRSSGVSDRIKSAATIMHSENQSLLAELRKSLIMMKEIGVDLEKDNQSRMVKEL 60
           MASTSNS S+GVS+RIKSAATIM+SENQSLLAELRKSLIMMKEIGV+LE+DNQSRMVKEL
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVELERDNQSRMVKEL 60

Query: 61  ENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQNHS 120
           ENSVVELLGTYE C+NFSSAIQSVGN YEP+EELTDFEKLLDDEVAK+SENSSSNLQNHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSMEC 180
           IIR FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELAEPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVRIQ 240
           KHVYEK  IMQYLKSK+SRAQCPVAACP+MLQPEKVISDPFL IEIDELRK S+HSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAD 249
           DFTE+DAD
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Lag0037862 vs. ExPASy TrEMBL
Match: A0A6J1IXZ2 (E3 SUMO-protein ligase MMS21 OS=Cucurbita maxima OX=3661 GN=LOC111480943 PE=3 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 1.0e-118
Identity = 225/250 (90.00%), Postives = 238/250 (95.20%), Query Frame = 0


Query: 1   MAST--SNSRSSGVSDRIKSAATIMHSENQSLLAELRKSLIMMKEIGVDLEKDNQSRMVK 60
           MAST  SNS S+GVS+RIKSAATIM+SENQSLLAELRKSLIMMKEIGVDLE+DNQSRMVK
Sbjct: 1   MASTSNSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVK 60

Query: 61  ELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQN 120
           ELENSVVELL TYE C+NFSSAIQSVGN YEP+EELTDFEKLLD+EVAK+SENSSSNLQN
Sbjct: 61  ELENSVVELLSTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQN 120

Query: 121 HSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSM 180
           HSIIR FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELAEPVRS 
Sbjct: 121 HSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRST 180

Query: 181 ECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVR 240
           ECKHVYEK  IMQYLKSK+SRAQCPVAACP+MLQPEKVISDPFL IEIDELRK S+HSVR
Sbjct: 181 ECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR 240

Query: 241 IQDFTELDAD 249
           IQDFTE+DAD
Sbjct: 241 IQDFTEVDAD 250

[Close]

BLAST of Lag0037862 vs. ExPASy TrEMBL
Match: A0A6J1CCS6 (E3 SUMO-protein ligase MMS21 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010470 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 2.8e-116
Identity = 217/248 (87.50%), Postives = 234/248 (94.35%), Query Frame = 0


Query: 1   MASTSNSRSSGVSDRIKSAATIMHSENQSLLAELRKSLIMMKEIGVDLEKDNQSRMVKEL 60
           MASTS++RSS VS RIKSAAT+MHSENQSLLAE+RK LIMMKEIG+DLE+DNQS MVK+L
Sbjct: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60

Query: 61  ENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQNHS 120
           EN+VVELL TYE+C+NFSSAIQSVGN YEP+EELTDFEKLLDDEVAK+SENSSSNLQNHS
Sbjct: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSMEC 180
           IIR FREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELA+PVRSMEC
Sbjct: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180

Query: 181 KHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVRIQ 240
           KHVYEK  +MQYL+SK SRAQCPVAACP+MLQPEKV  DPFL IEIDELRK SRHSVRIQ
Sbjct: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240

Query: 241 DFTELDAD 249
           DFTELDA+
Sbjct: 241 DFTELDAE 248

[Close]

BLAST of Lag0037862 vs. ExPASy TrEMBL
Match: A0A5D3DWX9 (E3 SUMO-protein ligase MMS21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001530 PE=3 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 2.4e-115
Identity = 215/248 (86.69%), Postives = 232/248 (93.55%), Query Frame = 0


Query: 1   MASTSNSRSSGVSDRIKSAATIMHSENQSLLAELRKSLIMMKEIGVDLEKDNQSRMVKEL 60
           MAS S+SRS+GV+ RIKSAATIMHSENQSLLAELRK+LIMMKEIGVDLEK+NQ++MVKEL
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSENQSLLAELRKTLIMMKEIGVDLEKENQNKMVKEL 60

Query: 61  ENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQNHS 120
           E S+VELL  YE+CNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAK+S NSSSN  NH 
Sbjct: 61  EKSMVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKVSVNSSSNFANHP 120

Query: 121 IIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSMEC 180
           IIR FREAIWNVHHAGQPM GEEQED+VMTSTQCNLLNVTCPLSGKPVTELAEPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMAGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSAEC 180

Query: 181 KHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVRIQ 240
           KH+YEKA IMQYL SKKSRAQCPVAACP+MLQP+KV+ DPFL+IEIDELRK+SRHS RIQ
Sbjct: 181 KHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLEIEIDELRKMSRHSERIQ 240

Query: 241 DFTELDAD 249
           DFTELDAD
Sbjct: 241 DFTELDAD 248

[Close]

BLAST of Lag0037862 vs. ExPASy TrEMBL
Match: A0A1S3CLF9 (E3 SUMO-protein ligase MMS21 OS=Cucumis melo OX=3656 GN=LOC103501788 PE=3 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 2.4e-115
Identity = 215/248 (86.69%), Postives = 232/248 (93.55%), Query Frame = 0


Query: 1   MASTSNSRSSGVSDRIKSAATIMHSENQSLLAELRKSLIMMKEIGVDLEKDNQSRMVKEL 60
           MAS S+SRS+GV+ RIKSAATIMHSENQSLLAELRK+LIMMKEIGVDLEK+NQ++MVKEL
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSENQSLLAELRKTLIMMKEIGVDLEKENQNKMVKEL 60

Query: 61  ENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQNHS 120
           E S+VELL  YE+CNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAK+S NSSSN  NH 
Sbjct: 61  EKSMVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKVSVNSSSNFANHP 120

Query: 121 IIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSMEC 180
           IIR FREAIWNVHHAGQPM GEEQED+VMTSTQCNLLNVTCPLSGKPVTELAEPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMAGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSAEC 180

Query: 181 KHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVRIQ 240
           KH+YEKA IMQYL SKKSRAQCPVAACP+MLQP+KV+ DPFL+IEIDELRK+SRHS RIQ
Sbjct: 181 KHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLEIEIDELRKMSRHSERIQ 240

Query: 241 DFTELDAD 249
           DFTELDAD
Sbjct: 241 DFTELDAD 248

[Close]

BLAST of Lag0037862 vs. TAIR 10
Match: AT3G15150.1 (RING/U-box superfamily protein )

HSP 1 Score: 275.4 bits (703), Expect = 4.5e-74
Identity = 142/243 (58.44%), Postives = 185/243 (76.13%), Query Frame = 0


Query: 5   SNSRSSGVSDRIKSAATIMHSENQSLLAELRKSLIMMKEIGVDLEKDNQSRMVKELENSV 64
           S S S GV+ RI++A+ ++ S+N S LA++RK++ MMK I V LEK+NQ+  VK+LENSV
Sbjct: 3   SASSSDGVAGRIQNASLVLVSDNSSTLADIRKAVAMMKNIAVQLEKENQTDKVKDLENSV 62

Query: 65  VELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQNHSIIRN 124
            ELL  +  CN+ S+AIQSV N Y+P E+LTDF+KLLDDE  K+    SS  QN  ++R 
Sbjct: 63  AELLDLHSDCNHRSTAIQSVANRYQPVEQLTDFKKLLDDEFTKLKATPSSVPQNDHLMRQ 122

Query: 125 FREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSMECKHVY 184
           FREA+WNVHHAG+PMPG++ EDIVMTSTQC LLN+TCPLSGKPVTELA+PVRSM+C+HVY
Sbjct: 123 FREAVWNVHHAGEPMPGDDDEDIVMTSTQCPLLNMTCPLSGKPVTELADPVRSMDCRHVY 182

Query: 185 EKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVR---IQD 244
           EK+VI+ Y+ +  + A CPVA C   LQ  KVI D  L+ EI+E+R +++ S R   I+D
Sbjct: 183 EKSVILHYIVNNPN-ANCPVAGCRGKLQNSKVICDAMLKFEIEEMRSLNKQSNRAEVIED 242

The following BLAST results are available for this feature:

BLAST of Lag0037862 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022941288.1	1.0e-120	91.13	E3 SUMO-protein ligase MMS21 [Cucurbita moschata]	[more]
XP_023525588.1	1.3e-120	91.13	E3 SUMO-protein ligase MMS21 [Cucurbita pepo subsp. pepo] >KAG7037426.1 E3 SUMO-...	[more]
KAG6607898.1	5.1e-120	90.73	E3 SUMO-protein ligase MMS21, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022981951.1	2.1e-118	90.00	E3 SUMO-protein ligase MMS21 [Cucurbita maxima]	[more]
XP_022139610.1	5.8e-116	87.50	E3 SUMO-protein ligase MMS21 isoform X1 [Momordica charantia]	[more]

BLAST of Lag0037862 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYH7	6.3e-73	58.44	E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana OX=3702 GN=MMS21 PE=1 SV=1	[more]

BLAST of Lag0037862 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FRP6	4.9e-121	91.13	E3 SUMO-protein ligase MMS21 OS=Cucurbita moschata OX=3662 GN=LOC111446631 PE=3 ...	[more]
A0A6J1IXZ2	1.0e-118	90.00	E3 SUMO-protein ligase MMS21 OS=Cucurbita maxima OX=3661 GN=LOC111480943 PE=3 SV...	[more]
A0A6J1CCS6	2.8e-116	87.50	E3 SUMO-protein ligase MMS21 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A5D3DWX9	2.4e-115	86.69	E3 SUMO-protein ligase MMS21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3CLF9	2.4e-115	86.69	E3 SUMO-protein ligase MMS21 OS=Cucumis melo OX=3656 GN=LOC103501788 PE=3 SV=1	[more]

BLAST of Lag0037862 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15150.1	4.5e-74	58.44	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004181	Zinc finger, MIZ-type	PFAM	PF11789	zf-Nse	coord: 145..207 e-value: 2.9E-14 score: 52.7
IPR004181	Zinc finger, MIZ-type	CDD	cd16651	SPL-RING_NSE2	coord: 160..228 e-value: 5.40373E-23 score: 86.9148
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 153..239 e-value: 8.6E-18 score: 66.4
IPR026846	E3 SUMO-protein ligase Nse2 (Mms21)	PANTHER	PTHR21330	UNCHARACTERIZED	coord: 5..235
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 159..231

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0037862.1	Lag0037862.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0060250	germ-line stem-cell niche homeostasis
biological_process	GO:0032876	negative regulation of DNA endoreduplication
biological_process	GO:0008284	positive regulation of cell population proliferation
biological_process	GO:0080038	positive regulation of cytokinin-activated signaling pathway
biological_process	GO:0045931	positive regulation of mitotic cell cycle
biological_process	GO:0016925	protein sumoylation
biological_process	GO:0048509	regulation of meristem development
biological_process	GO:0010082	regulation of root meristem growth
cellular_component	GO:0005634	nucleus
cellular_component	GO:0030915	Smc5-Smc6 complex
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0061665	SUMO ligase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0019789	SUMO transferase activity