Lag0037853 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0037853 vs. NCBI nr
Match: XP_023536443.1 (sodium/calcium exchanger NCL-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1031.9 bits (2667), Expect = 2.1e-297
Identity = 548/583 (94.00%), Postives = 566/583 (97.08%), Query Frame = 0

Query: 1   MSRPPLHLIFLLIFLLLCAQSHSSRFASISSVTSSDLVSDGVNDLQEPPYLRLNSLDSVS 60
           MSR PLHL+FLL FL+LC QSHSSRFASISS +SSDLVSDGVNDLQEP YLRLN+L S S
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISS-SSSDLVSDGVNDLQEPSYLRLNTLSSNS 60

Query: 61  APEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGLF 120
           APEESCEQSYGFLPCTT+ LGN+FLIIVYGYLMFLAA YLS GSELLLEILGPGIVGGLF
Sbjct: 61  APEESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLF 120

Query: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQ 180
           LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL+
Sbjct: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLE 180

Query: 181 DSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSGRHLAVL 240
            SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PF+IVQLPQMLNSTSGRHLAVL
Sbjct: 181 GSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLAVL 240

Query: 241 IALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD 300
           IALIIS+SMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD
Sbjct: 241 IALIISISMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD 300

Query: 301 KEIIDKLFSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDTSRDSHVD 360
           KEIIDKLFSRIDENKDG LSASELRALIVGIQFEELDLDH+DAV+KI+NDFDTSRDSHVD
Sbjct: 301 KEIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVD 360

Query: 361 SAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSDEVVEGV 420
           + EFGNGIIKWL+QVQG+RTGR DDGPHTMKFLH+FHQETKREHDLLDVGEQSDEVVE V
Sbjct: 361 ATEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDV 420

Query: 421 EEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA 480
           E GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA
Sbjct: 421 EGGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA 480

Query: 481 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEVLVILVV 540
           VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGL+WNFSSEVLVILVV
Sbjct: 481 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVV 540

Query: 541 TVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
           TV+MGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 TVIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 582

BLAST of Lag0037853 vs. NCBI nr
Match: XP_022976461.1 (sodium/calcium exchanger NCL-like [Cucurbita maxima])

HSP 1 Score: 1030.4 bits (2663), Expect = 6.0e-297
Identity = 546/583 (93.65%), Postives = 566/583 (97.08%), Query Frame = 0

Query: 1   MSRPPLHLIFLLIFLLLCAQSHSSRFASISSVTSSDLVSDGVNDLQEPPYLRLNSLDSVS 60
           MSR PLHL+FLL FL+LC QSHSSRFASISS  SSDLVSDGVN+LQEP YLRLN+L S S
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISS--SSDLVSDGVNELQEPSYLRLNTLSSNS 60

Query: 61  APEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGLF 120
           APEESCEQSYGFLPCTT+ LGN+FLIIVYGYLMFLAA YLS GSELLLEILGPGIVGGLF
Sbjct: 61  APEESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLF 120

Query: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQ 180
           LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL+
Sbjct: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLE 180

Query: 181 DSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSGRHLAVL 240
            SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV+PF+IVQLPQMLNSTSGRHLAVL
Sbjct: 181 GSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVL 240

Query: 241 IALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD 300
           IALIIS+SMF+IYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD
Sbjct: 241 IALIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD 300

Query: 301 KEIIDKLFSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDTSRDSHVD 360
           KEIIDKLFSRIDENKDG LSASELRALIVGIQFEELDLDH+DAV+KI+NDFDTSRDSHVD
Sbjct: 301 KEIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVD 360

Query: 361 SAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSDEVVEGV 420
           + EFGNGIIKWL+QVQG+RTGR DDGPHTMKFLH+FHQETKREHDLLDVGEQSDEVVE V
Sbjct: 361 ATEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDV 420

Query: 421 EEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA 480
           E GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA
Sbjct: 421 EGGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA 480

Query: 481 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEVLVILVV 540
           VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGL+WNFSSEVLVILVV
Sbjct: 481 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVV 540

Query: 541 TVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
           TV+MGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 TVIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of Lag0037853 vs. NCBI nr
Match: XP_022937179.1 (sodium/calcium exchanger NCL-like [Cucurbita moschata] >KAG7024799.1 Sodium/calcium exchanger NCL [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1029.6 bits (2661), Expect = 1.0e-296
Identity = 547/583 (93.83%), Postives = 565/583 (96.91%), Query Frame = 0

Query: 1   MSRPPLHLIFLLIFLLLCAQSHSSRFASISSVTSSDLVSDGVNDLQEPPYLRLNSLDSVS 60
           MSR PLHL+FLL FL+LC QSHSSRFASISS  SSDLVSDGVNDLQEP YLRLN+L S S
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISS--SSDLVSDGVNDLQEPSYLRLNTLPSNS 60

Query: 61  APEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGLF 120
           APEE+CEQSYGFLPCTT+ LGN+FLIIVYGYLMFLAA YLS GSELLLEILGPGIVGGLF
Sbjct: 61  APEEACEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLF 120

Query: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQ 180
           LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL+
Sbjct: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLE 180

Query: 181 DSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSGRHLAVL 240
            SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PF+IVQLPQMLNSTSGRHLAVL
Sbjct: 181 GSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLAVL 240

Query: 241 IALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD 300
           IALIIS+SMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD
Sbjct: 241 IALIISISMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD 300

Query: 301 KEIIDKLFSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDTSRDSHVD 360
           KEIIDKLFSRIDENKDG LSASELRALIVGIQFEELDLDH+DAV+KI+NDFDTSRDSHVD
Sbjct: 301 KEIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVD 360

Query: 361 SAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSDEVVEGV 420
           + EFGNGIIKWL+QVQG+RTGR DDGPHTMKFLH+FHQETKREHDLLDVGEQSDEVVE V
Sbjct: 361 ATEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDV 420

Query: 421 EEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA 480
           E GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA
Sbjct: 421 EGGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA 480

Query: 481 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEVLVILVV 540
           VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGL+WNFSSEVLVILVV
Sbjct: 481 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVV 540

Query: 541 TVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
           TV+MGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 TVIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of Lag0037853 vs. NCBI nr
Match: XP_038897650.1 (sodium/calcium exchanger NCL [Benincasa hispida])

HSP 1 Score: 1025.8 bits (2651), Expect = 1.5e-295
Identity = 545/587 (92.84%), Postives = 568/587 (96.76%), Query Frame = 0

Query: 1   MSRPPLHLIFLLIFLLLCAQSHSSRFASISSVT---SSDLVSDGVNDLQEPPYLRLNSLD 60
           MS  PLHL+F L FL+LC+QSHSSR ASISS+T   SSDLVSDGVN+LQEPPYL LN+L 
Sbjct: 1   MSSSPLHLLFFLFFLILCSQSHSSRLASISSLTSSSSSDLVSDGVNELQEPPYLHLNTLS 60

Query: 61  SVSAP-EESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIV 120
           S SAP EESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAA YLSTGSELLLEILGPGIV
Sbjct: 61  SFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIV 120

Query: 121 GGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGK 180
           GGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGK
Sbjct: 121 GGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGK 180

Query: 181 CDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSGRH 240
           CDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PF+IVQLPQMLNSTSGRH
Sbjct: 181 CDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRH 240

Query: 241 LAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTEN 300
           LAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQ+LGRLL EN
Sbjct: 241 LAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQSLGRLLREN 300

Query: 301 GEPDKEIIDKLFSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDTSRD 360
           GEPDKEIIDKLFS IDENKDGLLSASELRALIVGIQFEE+DLDHDDAV+KIMNDFDTSRD
Sbjct: 301 GEPDKEIIDKLFSSIDENKDGLLSASELRALIVGIQFEEIDLDHDDAVNKIMNDFDTSRD 360

Query: 361 SHVDSAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSDEV 420
           SHV++ EFGNGII+WL QVQG+R G+GDDGPHTMK+LH+FHQETKREHDLLDVGEQSDEV
Sbjct: 361 SHVNATEFGNGIIRWLTQVQGSRPGKGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEV 420

Query: 421 VEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATN 480
           VEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNAT+IPAFFISFIALPLATN
Sbjct: 421 VEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATN 480

Query: 481 SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEVLV 540
           SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGL+WNFSSEVLV
Sbjct: 481 SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLV 540

Query: 541 ILVVTVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
           ILVVT++MG+LGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 ILVVTIIMGVLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 587

BLAST of Lag0037853 vs. NCBI nr
Match: XP_004146032.1 (sodium/calcium exchanger NCL [Cucumis sativus] >KGN55087.1 hypothetical protein Csa_012240 [Cucumis sativus])

HSP 1 Score: 1024.6 bits (2648), Expect = 3.3e-295
Identity = 543/589 (92.19%), Postives = 569/589 (96.60%), Query Frame = 0

Query: 1   MSRPPLHLIFLLIFLLLCAQSHSSRFASISSVT-----SSDLVSDGVNDLQEPPYLRLNS 60
           MS  PLHL+FLL FL+LC+QS+SSR  SISS+T     SSDLVSDG+N LQEP YL LN+
Sbjct: 1   MSNSPLHLLFLLFFLVLCSQSYSSRLPSISSITSSSSSSSDLVSDGINGLQEPSYLHLNT 60

Query: 61  LDSVSAP-EESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPG 120
           L S+SAP EESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPG
Sbjct: 61  LSSLSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPG 120

Query: 121 IVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIV 180
           IVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIV
Sbjct: 121 IVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIV 180

Query: 181 GKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSG 240
           GKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV+PF+IVQLPQMLNSTSG
Sbjct: 181 GKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSG 240

Query: 241 RHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLT 300
           RHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLLT
Sbjct: 241 RHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLT 300

Query: 301 ENGEPDKEIIDKLFSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDTS 360
           ENGEPDKEII+KLFSRID NKDGLLSASELRALIVGIQF+E+DLDHDDAVDKIMNDFDTS
Sbjct: 301 ENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTS 360

Query: 361 RDSHVDSAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSD 420
           RDSHVDS EFGNGII+WL+QVQG+RTGRG+DGPHTMK+LH+FHQETKREHDLLDVGEQSD
Sbjct: 361 RDSHVDSNEFGNGIIRWLSQVQGSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSD 420

Query: 421 EVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLA 480
           EVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPLA
Sbjct: 421 EVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLA 480

Query: 481 TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEV 540
           TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGL+WNFSSEV
Sbjct: 481 TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEV 540

Query: 541 LVILVVTVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
           LVIL+VT++MG++GSFRTAFPLWT+LVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 LVILIVTMIMGVMGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 589

BLAST of Lag0037853 vs. ExPASy Swiss-Prot
Match: Q8L636 (Sodium/calcium exchanger NCL OS=Arabidopsis thaliana OX=3702 GN=NCL PE=2 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 1.3e-206
Identity = 394/578 (68.17%), Postives = 456/578 (78.89%), Query Frame = 0

Query: 12  LIFLLLCAQSHSSRFASISSVTSSDLVSDGVNDLQEPPYLRLNSLDSVSAP----EESCE 71
           L+FLL    S  +RF S++  +S+ L+SDG++               VSAP    EE+CE
Sbjct: 9   LLFLLFFTSSAYARFVSLNP-SSTSLISDGIDGGSNLAGSGSVIKSVVSAPAEEKEEACE 68

Query: 72  QSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGLFLPALGAL 131
           Q+YGF+PCT TALGN+FLI+VYG+LMF AATYLS GSELLLEILGPGIVGGLFLP LGAL
Sbjct: 69  QTYGFMPCTKTALGNVFLILVYGFLMFTAATYLSAGSELLLEILGPGIVGGLFLPMLGAL 128

Query: 132 PDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVAIDS 191
           PDAMLI+VSGL+G A  AQSQVSVGMGLLAGSTVMLLT+IWGTC +VGKCDL+DS+A+++
Sbjct: 129 PDAMLIMVSGLSGDAATAQSQVSVGMGLLAGSTVMLLTVIWGTCTVVGKCDLRDSIAVNN 188

Query: 192 QDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSGRHLAVLIALIISV 251
           QDTKGF L +SGV+ DIWTSYAARIM ISVIPF+IVQLPQML STSGR L+VLIALI+SV
Sbjct: 189 QDTKGFHLKDSGVTVDIWTSYAARIMAISVIPFVIVQLPQMLGSTSGRQLSVLIALILSV 248

Query: 252 SMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKEIIDKL 311
            M I YC+YQVFQPWIQRR+LAF KHKHVI G+LRHLKQ ALGRLL + G+PD+ +I KL
Sbjct: 249 LMLISYCVYQVFQPWIQRRRLAFAKHKHVISGILRHLKQHALGRLLDDEGQPDEHVIRKL 308

Query: 312 FSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDTSRDSHVDSAEFGNG 371
           F  ID N DG LSA+EL+ALI+GI FE++D D DDAV K++ DFD + D  VD  EF  G
Sbjct: 309 FLTIDANNDGHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQVDQEEFVRG 368

Query: 372 IIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSDEVVEG--VEEGKG 431
           I +WL Q  G      + GP TMKFL +FH +TKREH LL   E  +   EG  V + K 
Sbjct: 369 IKQWLIQAMGGAPSGPEAGPRTMKFLDNFHVQTKREHALLGDNENGENDEEGGEVADPKW 428

Query: 432 VLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVSAII 491
           + IKA L LLLG AIAAAFADPLVD V+NFS AT IP+FFISFIALPLATNSSEAVSAII
Sbjct: 429 ITIKAALLLLLGAAIAAAFADPLVDTVNNFSAATGIPSFFISFIALPLATNSSEAVSAII 488

Query: 492 FASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEVLVILVVTVVMG 551
           FASR K +TASLTFSEL G VTMNN+LCLSVFLA+VY+RGL WNFSSEVLVIL+V +VMG
Sbjct: 489 FASRKKIRTASLTFSELCGGVTMNNILCLSVFLAIVYVRGLTWNFSSEVLVILIVCLVMG 548

Query: 552 LLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
              SFRT +PLWT  +A LLYP SL LVY+LDY FGWS
Sbjct: 549 GFASFRTTYPLWTCFIAYLLYPFSLGLVYILDYWFGWS 585

BLAST of Lag0037853 vs. ExPASy Swiss-Prot
Match: Q5QNI2 (Sodium/calcium exchanger NCL1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL1 PE=2 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 3.9e-190
Identity = 367/585 (62.74%), Postives = 459/585 (78.46%), Query Frame = 0

Query: 8   LIFLLIFLL-LCAQSHSSRFASISSVTSSDLVSDGVNDLQEPPYLRLNSLDSVS------ 67
           L+ LL+FLL   A        S +S +++ L+S+ V+ +     +RL + +S S      
Sbjct: 10  LVPLLLFLLAAAAYGRLISDGSPASASATSLLSNPVSAV-----IRLTTSNSASASSPPA 69

Query: 68  -APEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGL 127
            APEE CEQSYGFLPCTTT LGNLFL++ YG+LM+ AAT+LS GSELLLEI+GPG+VGGL
Sbjct: 70  AAPEEKCEQSYGFLPCTTTVLGNLFLVLAYGFLMYKAATFLSAGSELLLEIMGPGLVGGL 129

Query: 128 FLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL 187
            LP LGALPDA+L+LVSGL+GS E AQSQV +GMGLLAGSTV LLTL+WGTCV+VGKCD+
Sbjct: 130 LLPILGALPDALLVLVSGLSGSRETAQSQVLIGMGLLAGSTVFLLTLLWGTCVVVGKCDI 189

Query: 188 -QDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSGRHLA 247
             + VA+D Q+ KGFSLT +G+STD+ TSYAARIM ISVIPFII Q P+ML +  G+ LA
Sbjct: 190 GPNGVAVDLQNNKGFSLTGTGISTDVQTSYAARIMGISVIPFIIAQFPKMLKTHHGQRLA 249

Query: 248 VLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGE 307
           VL+ALI+S S+ + YCLYQVFQPWIQ+RKLA+ KHKHVI G+LRH + +ALGRLL E+G 
Sbjct: 250 VLLALIVSFSLVLAYCLYQVFQPWIQKRKLAYAKHKHVISGILRHAQMEALGRLLNEDGT 309

Query: 308 PDKEIIDKLFSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDTSRDSH 367
           P++++I KLF +ID ++   LS +EL ALI+GI FEE+D D +DAVDKIM+DFDTS +  
Sbjct: 310 PNEDVIKKLFHKIDMDESQTLSRAELHALIIGINFEEVDFDKNDAVDKIMDDFDTSGNDI 369

Query: 368 VDSAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSDEVVE 427
           V+ AEF +G+ +WL +   A+      G ++ KF+  +H  T++EHDLL   ++SDE VE
Sbjct: 370 VEEAEFVSGMKRWLNE---AKRSVPTSGAYSNKFITDYHARTRQEHDLL--VDRSDETVE 429

Query: 428 GVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSS 487
            VE     + KA+  LLLG+AIAAAFADPLVD VHNFSNA++IP+FFISFIALPLATNSS
Sbjct: 430 SVENPGWCITKAVGLLLLGSAIAAAFADPLVDAVHNFSNASHIPSFFISFIALPLATNSS 489

Query: 488 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEVLVIL 547
           EAVSAIIFASR K +T+SLTFSE+YG VTMNN LCL VFLAL+Y+R L W+FSSEVL+IL
Sbjct: 490 EAVSAIIFASRKKLRTSSLTFSEVYGGVTMNNTLCLGVFLALIYIRNLTWDFSSEVLIIL 549

Query: 548 VVTVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
           +V V+MGL  SFRT FPLWT LVA +LYPLSLV+VY+LD+VFGWS
Sbjct: 550 LVCVIMGLFTSFRTTFPLWTCLVAYMLYPLSLVVVYILDFVFGWS 584

BLAST of Lag0037853 vs. ExPASy Swiss-Prot
Match: Q6K3R5 (Sodium/calcium exchanger NCL2 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL2 PE=2 SV=2)

HSP 1 Score: 633.6 bits (1633), Expect = 2.1e-180
Identity = 352/586 (60.07%), Postives = 440/586 (75.09%), Query Frame = 0

Query: 4   PPLHLIFLLIFLLLCAQSHSSRFASISSVTSSDLVSDG--VNDLQEPPYLRLNSLDSVSA 63
           PP   + +L+ LLL         A + +      +SDG   N       LRL S  + + 
Sbjct: 6   PPTRCLPVLLLLLLVV-------APLLAHGRRPFISDGGNANANANASVLRLPSAAAAAG 65

Query: 64  PEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGLFL 123
            +  CE SYGFLPCTTTA GNLFL++ YG+LMF +ATYLS+GSE+LL+ILGPGIVGGLFL
Sbjct: 66  EDMGCEMSYGFLPCTTTAWGNLFLVLAYGFLMFKSATYLSSGSEMLLQILGPGIVGGLFL 125

Query: 124 PALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL-Q 183
           P LGALPDA+LILVSGL+G+ EVAQSQV +GMGLLAGSTVMLLTL+WG+CV+VGKCDL +
Sbjct: 126 PILGALPDALLILVSGLSGTKEVAQSQVLIGMGLLAGSTVMLLTLLWGSCVVVGKCDLSE 185

Query: 184 DSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSGRHLAVL 243
           +S AIDS+DTKGFSL  SGVSTD  TSYAARIM IS++PFIIVQ+P++    SG  + VL
Sbjct: 186 NSTAIDSRDTKGFSLLGSGVSTDKQTSYAARIMAISILPFIIVQIPKIFKLHSGHQITVL 245

Query: 244 IALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD 303
           I LI++  + + YCLYQVFQPWIQRR+L + + KHV+ GLLRH ++ ++GRLL + G P+
Sbjct: 246 IGLIVAALLLLSYCLYQVFQPWIQRRRLEYTRLKHVMSGLLRHAQKHSIGRLLDDEGRPN 305

Query: 304 KEIIDKLFSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDTSRDSHVD 363
             +I+KLF RID++ DG L   EL+A IVGI FE++D + + A D++M DFDTSR+  ++
Sbjct: 306 VSVIEKLFHRIDQDNDGKLERGELQAFIVGINFEDIDWNSNLAADQVMADFDTSRNHFIE 365

Query: 364 SAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREH-DLLDVGEQSDEVVEG 423
             EF NG+++WL + +   T     G ++ KFL+ FH  T+ E   LLD  E+     EG
Sbjct: 366 KGEFVNGMLRWLDEAKRTVT----SGAYSKKFLNDFHARTRDEQTGLLDKDEE-----EG 425

Query: 424 VEEGK--GVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNS 483
             +G      IKAIL LLLGTA+AAA ADPLVD VHNFSNAT+IP+FFISFI +PLATNS
Sbjct: 426 EADGNPTWTCIKAILLLLLGTAMAAASADPLVDAVHNFSNATHIPSFFISFIVMPLATNS 485

Query: 484 SEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEVLVI 543
           SEAVSAIIFASR K++T SLTFSE+YG VTMNN LCL+VFLALVY+RGL W+FSSEVL+I
Sbjct: 486 SEAVSAIIFASRKKKRTLSLTFSEVYGGVTMNNTLCLAVFLALVYVRGLTWDFSSEVLII 545

Query: 544 LVVTVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
           L+V ++MGL  SFRT FPLWT  VA LLYPLSL++VY+LDY FGWS
Sbjct: 546 LLVCIIMGLFTSFRTDFPLWTCFVAFLLYPLSLIMVYILDYKFGWS 575

BLAST of Lag0037853 vs. ExPASy Swiss-Prot
Match: Q5KQN0 (Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX2 PE=2 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 1.7e-04
Identity = 43/139 (30.94%), Postives = 72/139 (51.80%), Query Frame = 0

Query: 409 VGEQSDEVVEGVEEGKGVLI-KAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFIS 468
           +GEQ +EV E  EE K +   +AI +L + T   +  +  LVD +   S + N+P  FIS
Sbjct: 261 IGEQEEEVTEDEEEEKEITQGEAICWLFVLTIWISILSGYLVDAIQGASESLNMPVAFIS 320

Query: 469 FIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRG-- 528
            I LP+  N++E  SAI+FA +DK     +T     G+ T  ++  +   + + ++ G  
Sbjct: 321 VILLPIVGNAAEHASAIMFAMKDK---LDITLGVAIGSSTQISMFVIPFCVVIGWIMGQQ 380

Query: 529 LIWNFSSEVLVILVVTVVM 545
           +  NF       L +TV++
Sbjct: 381 MDLNFQLFETATLFITVLV 396

BLAST of Lag0037853 vs. ExPASy TrEMBL
Match: A0A6J1IGZ1 (sodium/calcium exchanger NCL-like OS=Cucurbita maxima OX=3661 GN=LOC111476856 PE=4 SV=1)

HSP 1 Score: 1030.4 bits (2663), Expect = 2.9e-297
Identity = 546/583 (93.65%), Postives = 566/583 (97.08%), Query Frame = 0

Query: 1   MSRPPLHLIFLLIFLLLCAQSHSSRFASISSVTSSDLVSDGVNDLQEPPYLRLNSLDSVS 60
           MSR PLHL+FLL FL+LC QSHSSRFASISS  SSDLVSDGVN+LQEP YLRLN+L S S
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISS--SSDLVSDGVNELQEPSYLRLNTLSSNS 60

Query: 61  APEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGLF 120
           APEESCEQSYGFLPCTT+ LGN+FLIIVYGYLMFLAA YLS GSELLLEILGPGIVGGLF
Sbjct: 61  APEESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLF 120

Query: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQ 180
           LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL+
Sbjct: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLE 180

Query: 181 DSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSGRHLAVL 240
            SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV+PF+IVQLPQMLNSTSGRHLAVL
Sbjct: 181 GSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVL 240

Query: 241 IALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD 300
           IALIIS+SMF+IYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD
Sbjct: 241 IALIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD 300

Query: 301 KEIIDKLFSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDTSRDSHVD 360
           KEIIDKLFSRIDENKDG LSASELRALIVGIQFEELDLDH+DAV+KI+NDFDTSRDSHVD
Sbjct: 301 KEIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVD 360

Query: 361 SAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSDEVVEGV 420
           + EFGNGIIKWL+QVQG+RTGR DDGPHTMKFLH+FHQETKREHDLLDVGEQSDEVVE V
Sbjct: 361 ATEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDV 420

Query: 421 EEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA 480
           E GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA
Sbjct: 421 EGGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA 480

Query: 481 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEVLVILVV 540
           VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGL+WNFSSEVLVILVV
Sbjct: 481 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVV 540

Query: 541 TVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
           TV+MGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 TVIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of Lag0037853 vs. ExPASy TrEMBL
Match: A0A6J1FAG1 (sodium/calcium exchanger NCL-like OS=Cucurbita moschata OX=3662 GN=LOC111443554 PE=4 SV=1)

HSP 1 Score: 1029.6 bits (2661), Expect = 4.9e-297
Identity = 547/583 (93.83%), Postives = 565/583 (96.91%), Query Frame = 0

Query: 1   MSRPPLHLIFLLIFLLLCAQSHSSRFASISSVTSSDLVSDGVNDLQEPPYLRLNSLDSVS 60
           MSR PLHL+FLL FL+LC QSHSSRFASISS  SSDLVSDGVNDLQEP YLRLN+L S S
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISS--SSDLVSDGVNDLQEPSYLRLNTLPSNS 60

Query: 61  APEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGLF 120
           APEE+CEQSYGFLPCTT+ LGN+FLIIVYGYLMFLAA YLS GSELLLEILGPGIVGGLF
Sbjct: 61  APEEACEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLF 120

Query: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQ 180
           LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL+
Sbjct: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLE 180

Query: 181 DSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSGRHLAVL 240
            SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PF+IVQLPQMLNSTSGRHLAVL
Sbjct: 181 GSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLAVL 240

Query: 241 IALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD 300
           IALIIS+SMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD
Sbjct: 241 IALIISISMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD 300

Query: 301 KEIIDKLFSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDTSRDSHVD 360
           KEIIDKLFSRIDENKDG LSASELRALIVGIQFEELDLDH+DAV+KI+NDFDTSRDSHVD
Sbjct: 301 KEIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVD 360

Query: 361 SAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSDEVVEGV 420
           + EFGNGIIKWL+QVQG+RTGR DDGPHTMKFLH+FHQETKREHDLLDVGEQSDEVVE V
Sbjct: 361 ATEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDV 420

Query: 421 EEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA 480
           E GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA
Sbjct: 421 EGGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA 480

Query: 481 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEVLVILVV 540
           VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGL+WNFSSEVLVILVV
Sbjct: 481 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVV 540

Query: 541 TVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
           TV+MGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 TVIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of Lag0037853 vs. ExPASy TrEMBL
Match: A0A0A0L3B8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G628350 PE=4 SV=1)

HSP 1 Score: 1024.6 bits (2648), Expect = 1.6e-295
Identity = 543/589 (92.19%), Postives = 569/589 (96.60%), Query Frame = 0

Query: 1   MSRPPLHLIFLLIFLLLCAQSHSSRFASISSVT-----SSDLVSDGVNDLQEPPYLRLNS 60
           MS  PLHL+FLL FL+LC+QS+SSR  SISS+T     SSDLVSDG+N LQEP YL LN+
Sbjct: 1   MSNSPLHLLFLLFFLVLCSQSYSSRLPSISSITSSSSSSSDLVSDGINGLQEPSYLHLNT 60

Query: 61  LDSVSAP-EESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPG 120
           L S+SAP EESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPG
Sbjct: 61  LSSLSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPG 120

Query: 121 IVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIV 180
           IVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIV
Sbjct: 121 IVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIV 180

Query: 181 GKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSG 240
           GKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV+PF+IVQLPQMLNSTSG
Sbjct: 181 GKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSG 240

Query: 241 RHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLT 300
           RHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLLT
Sbjct: 241 RHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLT 300

Query: 301 ENGEPDKEIIDKLFSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDTS 360
           ENGEPDKEII+KLFSRID NKDGLLSASELRALIVGIQF+E+DLDHDDAVDKIMNDFDTS
Sbjct: 301 ENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTS 360

Query: 361 RDSHVDSAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSD 420
           RDSHVDS EFGNGII+WL+QVQG+RTGRG+DGPHTMK+LH+FHQETKREHDLLDVGEQSD
Sbjct: 361 RDSHVDSNEFGNGIIRWLSQVQGSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSD 420

Query: 421 EVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLA 480
           EVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPLA
Sbjct: 421 EVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLA 480

Query: 481 TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEV 540
           TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGL+WNFSSEV
Sbjct: 481 TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEV 540

Query: 541 LVILVVTVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
           LVIL+VT++MG++GSFRTAFPLWT+LVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 LVILIVTMIMGVMGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 589

BLAST of Lag0037853 vs. ExPASy TrEMBL
Match: A0A6J1FT50 (sodium/calcium exchanger NCL-like OS=Cucurbita moschata OX=3662 GN=LOC111446626 PE=4 SV=1)

HSP 1 Score: 1024.2 bits (2647), Expect = 2.1e-295
Identity = 541/583 (92.80%), Postives = 570/583 (97.77%), Query Frame = 0

Query: 1   MSRPPLHLIFLLIFLLLCAQSHSSRFASISSVTSSDLVSDGVNDLQEPPYLRLNSLDSVS 60
           MSRPPL+LIFLLIF +LC QSHSSRFASISS  SSDLVSDGVN+LQE PYLRLNSL+SVS
Sbjct: 1   MSRPPLYLIFLLIFFILCGQSHSSRFASISS--SSDLVSDGVNELQETPYLRLNSLESVS 60

Query: 61  APEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGLF 120
           APEESCE+SYGFLPCTT+ALGNLFLIIVYGYLMFLAA YLSTGSELLLEILGPGIVGGLF
Sbjct: 61  APEESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLF 120

Query: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQ 180
           LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQ
Sbjct: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQ 180

Query: 181 DSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSGRHLAVL 240
           DSVAID QDTKGFSLTESGVSTDIWTSYAARIMVISV+PF++VQLPQ+LNSTSGRHLAVL
Sbjct: 181 DSVAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVL 240

Query: 241 IALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD 300
           IALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTE+GEPD
Sbjct: 241 IALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTESGEPD 300

Query: 301 KEIIDKLFSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDTSRDSHVD 360
           +EII+KLFSRID NK+GLLSASELRALIVGIQFE +DLDHDDAV+KI++DFDTSRD+HVD
Sbjct: 301 REIIEKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVD 360

Query: 361 SAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSDEVVEGV 420
           S EF NGII+WLAQVQGARTGR DDGPHT+K+LH+FHQETKREHDLLDVGEQSDEVVEGV
Sbjct: 361 STEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGV 420

Query: 421 EEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEA 480
           EE KGVLI+AILFLLLGTAIAAAFADPLVDVVHNFSNAT+IPAFFISFIALPLATNSSEA
Sbjct: 421 EESKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEA 480

Query: 481 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEVLVILVV 540
           VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGL+WNFSSEVLVILVV
Sbjct: 481 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVV 540

Query: 541 TVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
           T++MGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 TMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of Lag0037853 vs. ExPASy TrEMBL
Match: A0A5A7VFE4 (Sodium/calcium exchanger family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001180 PE=4 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.8e-294
Identity = 543/590 (92.03%), Postives = 567/590 (96.10%), Query Frame = 0

Query: 1   MSRPPLHLIFLLIFLLLCAQSHSSRFASISSVT------SSDLVSDGVNDLQEPPYLRLN 60
           MS+ PLHL+FLL FL+LC+QS+SSR  SISS+T      SSDLVSDGVN+LQEP YL LN
Sbjct: 1   MSKSPLHLLFLLFFLVLCSQSYSSRLPSISSLTSSSSSSSSDLVSDGVNELQEPSYLHLN 60

Query: 61  SLDSVSAP-EESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGP 120
           +L S SAP EESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAA YLSTGSELLLEILGP
Sbjct: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120

Query: 121 GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 180
           GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI
Sbjct: 121 GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 180

Query: 181 VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTS 240
           VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV+PF+IVQLPQMLNSTS
Sbjct: 181 VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTS 240

Query: 241 GRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLL 300
           GRHLAVLIALI+SVSMFI YCLYQVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLL
Sbjct: 241 GRHLAVLIALIVSVSMFITYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLL 300

Query: 301 TENGEPDKEIIDKLFSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDT 360
           TENGEPDKEII+KLFSRID NKDGLLSASELRALIVGIQF+E+DLDHDDAVDKIMNDFDT
Sbjct: 301 TENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDT 360

Query: 361 SRDSHVDSAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQS 420
           SRDSHVDS EFGNGII+WL+QVQG+RTGRGDDGPHTMK+LH+FHQETKREHDLLDVGEQS
Sbjct: 361 SRDSHVDSNEFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQS 420

Query: 421 DEVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPL 480
           DEVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPL
Sbjct: 421 DEVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPL 480

Query: 481 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSE 540
           ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGL+WNFSSE
Sbjct: 481 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSE 540

Query: 541 VLVILVVTVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
           VLVILVVTVVMG++GSFRTAFPLWT+LVALLLYP SLVLVYVLDYVFGWS
Sbjct: 541 VLVILVVTVVMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 590

BLAST of Lag0037853 vs. TAIR 10
Match: AT1G53210.1 (sodium/calcium exchanger family protein / calcium-binding EF hand family protein )

HSP 1 Score: 720.7 bits (1859), Expect = 9.5e-208
Identity = 394/578 (68.17%), Postives = 456/578 (78.89%), Query Frame = 0

Query: 12  LIFLLLCAQSHSSRFASISSVTSSDLVSDGVNDLQEPPYLRLNSLDSVSAP----EESCE 71
           L+FLL    S  +RF S++  +S+ L+SDG++               VSAP    EE+CE
Sbjct: 9   LLFLLFFTSSAYARFVSLNP-SSTSLISDGIDGGSNLAGSGSVIKSVVSAPAEEKEEACE 68

Query: 72  QSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGLFLPALGAL 131
           Q+YGF+PCT TALGN+FLI+VYG+LMF AATYLS GSELLLEILGPGIVGGLFLP LGAL
Sbjct: 69  QTYGFMPCTKTALGNVFLILVYGFLMFTAATYLSAGSELLLEILGPGIVGGLFLPMLGAL 128

Query: 132 PDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVAIDS 191
           PDAMLI+VSGL+G A  AQSQVSVGMGLLAGSTVMLLT+IWGTC +VGKCDL+DS+A+++
Sbjct: 129 PDAMLIMVSGLSGDAATAQSQVSVGMGLLAGSTVMLLTVIWGTCTVVGKCDLRDSIAVNN 188

Query: 192 QDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSGRHLAVLIALIISV 251
           QDTKGF L +SGV+ DIWTSYAARIM ISVIPF+IVQLPQML STSGR L+VLIALI+SV
Sbjct: 189 QDTKGFHLKDSGVTVDIWTSYAARIMAISVIPFVIVQLPQMLGSTSGRQLSVLIALILSV 248

Query: 252 SMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKEIIDKL 311
            M I YC+YQVFQPWIQRR+LAF KHKHVI G+LRHLKQ ALGRLL + G+PD+ +I KL
Sbjct: 249 LMLISYCVYQVFQPWIQRRRLAFAKHKHVISGILRHLKQHALGRLLDDEGQPDEHVIRKL 308

Query: 312 FSRIDENKDGLLSASELRALIVGIQFEELDLDHDDAVDKIMNDFDTSRDSHVDSAEFGNG 371
           F  ID N DG LSA+EL+ALI+GI FE++D D DDAV K++ DFD + D  VD  EF  G
Sbjct: 309 FLTIDANNDGHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQVDQEEFVRG 368

Query: 372 IIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSDEVVEG--VEEGKG 431
           I +WL Q  G      + GP TMKFL +FH +TKREH LL   E  +   EG  V + K 
Sbjct: 369 IKQWLIQAMGGAPSGPEAGPRTMKFLDNFHVQTKREHALLGDNENGENDEEGGEVADPKW 428

Query: 432 VLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVSAII 491
           + IKA L LLLG AIAAAFADPLVD V+NFS AT IP+FFISFIALPLATNSSEAVSAII
Sbjct: 429 ITIKAALLLLLGAAIAAAFADPLVDTVNNFSAATGIPSFFISFIALPLATNSSEAVSAII 488

Query: 492 FASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEVLVILVVTVVMG 551
           FASR K +TASLTFSEL G VTMNN+LCLSVFLA+VY+RGL WNFSSEVLVIL+V +VMG
Sbjct: 489 FASRKKIRTASLTFSELCGGVTMNNILCLSVFLAIVYVRGLTWNFSSEVLVILIVCLVMG 548

Query: 552 LLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 584
              SFRT +PLWT  +A LLYP SL LVY+LDY FGWS
Sbjct: 549 GFASFRTTYPLWTCFIAYLLYPFSLGLVYILDYWFGWS 585

BLAST of Lag0037853 vs. TAIR 10
Match: AT1G29025.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 237.7 bits (605), Expect = 2.4e-62
Identity = 187/598 (31.27%), Postives = 302/598 (50.50%), Query Frame = 0

Query: 1   MSRPPLHLIFLLIFLLLCAQSHSSRFASISSVTSSDLVSDGVNDLQEPPYLRLNSLDSVS 60
           M+R  L L FL + L+           S  +  SS LVSDGV D     +L   SL+  +
Sbjct: 1   MARLTLFLSFLSLALVSVVNCRVLSSVSEDN-NSSILVSDGVQDRSSNDFL---SLNPPN 60

Query: 61  APEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGLF 120
             E +C   YGFLPC     G +F +  +G L+ +   +LS G   L  I   G  GG+ 
Sbjct: 61  LSESACVHVYGFLPCADNIEGYVFQVFSFGCLLIIGEYFLSKGRSKLFVIFEVGFFGGII 120

Query: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQ 180
            P L   P   LIL +GL GS E+A S     + +  G +V  LT+ WG CV+ G   L+
Sbjct: 121 FPLLTMFPRIALILSTGLIGSREIANSMTGNNVAVTVGYSVFALTMQWGACVVFGLSGLR 180

Query: 181 ---DSVAID-----SQDTKGFSLTE----SGVSTDIWTSYAARIMVISVIPFIIVQLPQM 240
               S+  D      Q  K  +L +    + +  D     AA IM+++++PF++V   + 
Sbjct: 181 SDDQSITCDISSPRRQVKKSINLLKNLAGASIRADPKNKKAAGIMLLTLLPFVLVTFSET 240

Query: 241 LNSTSGRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVK-------HKHVIFGLL 300
            +  S     VL+ LI S S  ++Y +Y  F    Q + L + K       HKH     L
Sbjct: 241 FHIKSWDDNMVLVTLIFSGSATVLYFVYLYFDKADQVKSLEYAKFELMSEVHKH-----L 300

Query: 301 RHLKQQALGRLLTENGEPDKEIIDKLFSRIDENKDGLLSASELRALIVGI-QFEELDLDH 360
           ++   Q+L R    NG+  +E +  LF + D NKDG +  SEL+ L V       +  D 
Sbjct: 301 QNFSPQSLIR----NGQLCQESLKSLFKKFDVNKDGKIQVSELKDLTVDFGVLGRVKCDI 360

Query: 361 DDAVDKIMNDFDTSRDSHVDSAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQET 420
           ++    ++ DFD++RD  +D  EF  GI KWL Q + +  G    G           ++ 
Sbjct: 361 NELATTLLADFDSNRDGEIDETEFAIGIEKWLKQYKFSFNGTESQG-----------EDI 420

Query: 421 KREHDLLDVGEQSDEVVEGVEEGKGV---LIKAILFLLLGTAIAAAFADPLVDVVHNFSN 480
             +  +L V    +++++G    K +    +KA++ +++G  I +  A P +  +   S 
Sbjct: 421 AEDDGVLKV----EQLLKGCLFTKLLTKRTLKAVIEVIIGITIVSFLAMPFMMNIELLSV 480

Query: 481 ATNIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVF 540
           +  +P+F++ F  +PLA N   A+SA     ++K K  S TFSE+Y  VT+NN+L +++ 
Sbjct: 481 SAGVPSFYVVFAVIPLARNLKNALSAHFCRKKEKAKITSDTFSEIYRDVTLNNLLGITII 540

Query: 541 LALVYMRGLIWNFSSEVLVILVVTVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYV 576
           LA+VY+RGL W++S+EVL+I++V +++G+    R+ +P W  ++A  LY  SL+L+Y+
Sbjct: 541 LAIVYIRGLTWDYSTEVLIIVIVGLIIGVPAYVRSTYPFWICVLAFALYFFSLILIYL 570

BLAST of Lag0037853 vs. TAIR 10
Match: AT1G29020.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 234.6 bits (597), Expect = 2.1e-61
Identity = 180/575 (31.30%), Postives = 292/575 (50.78%), Query Frame = 0

Query: 1   MSRPPLHLIFLLIFLLLCAQSHSSRFASISSVTSSDLVSDGVNDLQEPPYLRLNSLDSVS 60
           M+R  L L FL +  L+     + R  S  S   S LVSDGV D     +L L+  + ++
Sbjct: 1   MARLSLFLSFLSLIALISVV--NCRVLSSLSEKDSILVSDGVQDGSSYEFLSLDPPNDIT 60

Query: 61  APEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGLF 120
             +  C   Y FLPC     G +F +  +G ++ +   +L+ G   L  I   G  GG+ 
Sbjct: 61  --KNMCVHVYDFLPCADNVAGYVFQVFSFGCILIIGEYFLTKGRTKLFLIFEVGFYGGII 120

Query: 121 LPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQ 180
            P L   P   LIL +GL GS E+A S+V   +G+  G +V  LT+ WG C++ G    +
Sbjct: 121 FPLLTMFPRIALILSTGLVGSREMASSRVGNIIGVTVGYSVFALTIQWGACILFGLSGTK 180

Query: 181 DSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSGRHLAVL 240
               ID        L+ + V  DI    +A IM+++++PFI+V   ++ +S     + VL
Sbjct: 181 PEQPID--------LSAASVEADITNKKSAGIMLLTILPFILVTFSEISSSQYWNDIIVL 240

Query: 241 IALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPD 300
           I LIIS S    Y  Y  F    Q + L   + + ++  + +HL Q    + L  +G+ +
Sbjct: 241 ITLIISASATFAYFFYSYFDRDDQAKSLDHARFE-LMSEVHKHL-QIFSPKSLIRDGQLN 300

Query: 301 KEIIDKLFSRIDENKDGLLSASELRALIVGI-QFEELDLDHDDAVDKIMNDFDTSRDSHV 360
           +E + +LF +ID N DG +  +EL+ L V       +    D  V  +++DFD   D  +
Sbjct: 301 QESLKRLFQKIDANGDGKIQVAELKDLTVEFGMIGRVKCHIDKLVHTLVDDFDRDGDREI 360

Query: 361 DSAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSDEVVEG 420
           D AEF  GI KWL Q +            +        ++      +L V +    +V+ 
Sbjct: 361 DEAEFEIGIKKWLNQYK-----------FSFDTTVPPREDQAEGVPILKVEKPEQSLVKK 420

Query: 421 VEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSE 480
           +   +   ++A L ++LG  +    A P +  +   S +  +P+F++ F+ +PLA N   
Sbjct: 421 LLSWR--TLRASLEVILGMLVVIYLARPFMMNIQLLSESAGVPSFYVVFVVIPLARNLKN 480

Query: 481 AVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSSEVLVILV 540
            +SA     +DK K  S TFSE+Y  VTMNN++ +S+ LA+VY R L+W++++EVL+I++
Sbjct: 481 TLSAHFCRKKDKAKITSDTFSEIYKDVTMNNLMGISIILAIVYARELVWDYTTEVLIIVI 540

Query: 541 VTVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVY 575
           V +++GL    RT +P W  L+A  LY +SLV+VY
Sbjct: 541 VGLLIGLPAYVRTTYPFWICLLAFALYFISLVVVY 548

BLAST of Lag0037853 vs. TAIR 10
Match: AT2G34020.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 232.6 bits (592), Expect = 7.8e-61
Identity = 195/621 (31.40%), Postives = 305/621 (49.11%), Query Frame = 0

Query: 5   PLHLIFLLIFLLLCAQSHSSRFASISSVTSSDLVSDGVNDLQEPPYLRLNSLDSVSAPEE 64
           P  L F  + L L     SSR  S   + +S L+SDGV+   +  YL L+   +VS  + 
Sbjct: 16  PSTLFFFFLSLTLFISGVSSRVLSPVPLDNSSLISDGVHGASDYKYLTLDPPKNVS--KA 75

Query: 65  SCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGLFLPAL 124
           +C   YGFLPC     G +F +  +G L+ +   +LS G   L  I   G  GG+  P L
Sbjct: 76  ACIHVYGFLPCADNIGGYVFQVFSFGCLLIIGDYFLSQGRSKLFVIFEVGFYGGIVFPLL 135

Query: 125 GALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD------ 184
              P   L++  GLA + E A   V   +G+  G T+  LT+ WG CVI G         
Sbjct: 136 TMFPRIALMISPGLAATHEGALMIVGNNVGVTIGHTIFALTMQWGACVIFGLTSPNSDPS 195

Query: 185 -LQDSVAIDSQDTK----GF-------SLTESGVSTDIWTSYAARIMVISVIPFIIVQLP 244
             + S+   S DTK    GF       S+ E+ V  D     AA IM+++++PF++V LP
Sbjct: 196 IRRGSIKRTSSDTKNPRRGFYRMKILKSVVEASVDADPKNKKAAGIMLLTLVPFLLVTLP 255

Query: 245 QMLNSTSGRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQ 304
            +L+  S   + +LI LIIS S   IY +Y  F    Q++ L   K + ++  + +HL Q
Sbjct: 256 DLLDVQSWSDIIMLITLIISCSSTFIYFVYSYFDTADQKKSLDHAKFE-LMSEVHKHL-Q 315

Query: 305 QALGRLLTENGEPDKEIIDKLFSRIDENKDGLLSASELRALIVGI-QFEELDLDHDDAVD 364
               R L  +G+  KE +  LF +ID NKDG +  SEL+ L V    F ++  D ++   
Sbjct: 316 SFSPRTLIRDGQLSKESLKSLFDKIDRNKDGKIQISELKDLTVEFGVFGKMKCDINEFAS 375

Query: 365 KIMNDFDTSRDSHVDSAEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHF---HQETKR 424
            ++ +FD  ++  +D  EF  GI                     MK L+H+   +QE+ R
Sbjct: 376 TLLAEFDKDKNGELDENEFEEGI---------------------MKLLNHYKFDNQESPR 435

Query: 425 EHDLLDVGEQSDEVVEGVEEGK--GVL------------------IKAILFLLLGTAIAA 484
           +++       SD  V+ + +G+  GVL                  ++A+  ++ G  I  
Sbjct: 436 QNNTYIYRTPSDS-VKSLSQGEEAGVLKLEMPKQTLVAKFLSMSTLRAVTKVIGGMLIVV 495

Query: 485 AFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSEL 544
             A P +  +   S +  +P+F+  F  +PL  N    +SA     +DK + AS  FSE+
Sbjct: 496 FLAKPFMVNIGLLSVSAGVPSFYSVFAVIPLVRNLKNTLSAHFCRKKDKARIASEKFSEI 555

Query: 545 YGAVTMNNVLCLSVFLALVYMRGLIWNFSSEVLVILVVTVVMGLLGSFRTAFPLWTALVA 584
           Y  VTMNN++ +S+ LA+VY RGL W +S E L+++VV + +GL    R+ +P W  ++A
Sbjct: 556 YRDVTMNNLMGMSITLAIVYSRGLKWEYSIESLLVVVVGIAIGLPAYVRSTYPFWICVMA 610

BLAST of Lag0037853 vs. TAIR 10
Match: AT2G34030.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 231.9 bits (590), Expect = 1.3e-60
Identity = 182/583 (31.22%), Postives = 293/583 (50.26%), Query Frame = 0

Query: 8   LIFLLIFLLLCAQSHSSR-FASISSVTSSDLVSDGVNDLQEPPYLRLNSLDSVSAPEESC 67
           L F L+ + L     SSR   S   + +S L+SDG++D     +L L+   S+S    +C
Sbjct: 6   LFFSLLSIALIISGVSSRVLISHVPLNNSILISDGIHDALNHEFLTLDPPKSLS--RTAC 65

Query: 68  EQSYGFLPCTTTALGNLFLIIVYGYLMFLAATYLSTGSELLLEILGPGIVGGLFLPALGA 127
              YGFLPC     G +F +  +G L+ +   +LS G   L  I   G  GG+  P L  
Sbjct: 66  VHVYGFLPCADNVEGYIFQVFSFGSLLIIGDYFLSEGRSKLFVIFEVGFYGGIIFPLLTM 125

Query: 128 LPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVG----KCDLQDS 187
            P   L+L +GL+ S +VA S +   +GL  G TV  LT+ WG CV+      + D  D 
Sbjct: 126 FPRIALMLSTGLSLSRDVASSFIDDNVGLTVGHTVFSLTIQWGACVVFSITGPRSDQADG 185

Query: 188 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQMLNSTSGRHLAVLIA 247
           +    +  KGF   E+ V  D     AA IM++S+ PF++V    + +S S  H+ VLI 
Sbjct: 186 LIEKLKILKGF--VEARVEADPKNKKAAGIMLLSLSPFLMVTFSAIFDSHSWSHIIVLIT 245

Query: 248 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 307
           LIIS S  ++Y +Y       Q + L   + + ++  + +HLK+ +   L+ ++GE  KE
Sbjct: 246 LIISSSSTVVYFVYSYLDTANQEKSLDHARFE-LMSEVHKHLKRFSPKHLI-KDGELSKE 305

Query: 308 IIDKLFSRIDENKDGLLSASELRALIVGI-QFEELDLDHDDAVDKIMNDFDTSRDSHVDS 367
            +  LF + D+NKDG +  SEL+ L + +  F  +  D ++     + DFD   D  ++ 
Sbjct: 306 SLKSLFKKTDKNKDGKIQISELKDLTIELSNFGRMRYDINELAKAFLEDFDGDNDGELEE 365

Query: 368 AEFGNGIIKWLAQVQGARTGRGDDGPHTMKFLHHFHQETKREHDLLDVGEQSDEVVEGVE 427
            EF  GI + L Q                   + F+ E +RE+   + G    E+     
Sbjct: 366 NEFEEGIARLLKQ-------------------YKFNVEDQRENQTEENGVLKLEI----- 425

Query: 428 EGKGVLIKAILFL---------LLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALP 487
           + K  L+  +L +         ++G  I    A P +  +   S +  IP+F+I F  +P
Sbjct: 426 KPKKTLVTKLLSMETLIATTEVIVGILIVLFLAKPFMLNIQLLSISAGIPSFYIVFAMIP 485

Query: 488 LATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLIWNFSS 547
            A N    +S      +DK++ +S TFSE+Y  +TMNN+L +SV LA+VY RGL W +S 
Sbjct: 486 FARNLKNTLSTRFCRGKDKKRVSSNTFSEIYKDITMNNLLGMSVILAIVYTRGLTWKYSV 545

Query: 548 EVLVILVVTVVMGLLGSFRTAFPLWTALVALLLYPLSLVLVYV 576
           E L++++V +++GL    R+ +P W  ++A  +Y  SL+L+Y+
Sbjct: 546 ETLIMVIVGLIIGLPIYIRSTYPFWMCVLAFAMYFFSLLLIYI 558