Lag0037683 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0037683 vs. NCBI nr
Match: XP_038897182.1 (vacuolar protein sorting-associated protein 53 A [Benincasa hispida] >XP_038897183.1 vacuolar protein sorting-associated protein 53 A [Benincasa hispida])

HSP 1 Score: 1511.9 bits (3913), Expect = 0.0e+00
Identity = 794/807 (98.39%), Postives = 798/807 (98.88%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI
Sbjct: 17   EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 76

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA
Sbjct: 77   KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 136

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 1063
            AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL
Sbjct: 137  AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 196

Query: 1064 QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 1123
            QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR
Sbjct: 197  QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 256

Query: 1124 TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFEDE 1183
            TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDI+DNLKEKPDVATLLLALQRTLEFEDE
Sbjct: 257  TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDVATLLLALQRTLEFEDE 316

Query: 1184 LAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKDGIKDMS 1243
            LAEKFGGG RGKE+GNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEK+GIKDM 
Sbjct: 317  LAEKFGGGARGKESGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKNGIKDMP 376

Query: 1244 VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 1303
            VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ
Sbjct: 377  VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 436

Query: 1304 LFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQ 1363
            LFLIIKRSLKRCSALTKNQTL NLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQ
Sbjct: 437  LFLIIKRSLKRCSALTKNQTLLNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQ 496

Query: 1364 IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA 1423
            IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVD VDMSEVQDEFSAVITKA
Sbjct: 497  IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDAVDMSEVQDEFSAVITKA 556

Query: 1424 LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 1483
            LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF
Sbjct: 557  LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 616

Query: 1484 QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSSAASY 1543
            QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTS A SY
Sbjct: 617  QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSGATSY 676

Query: 1544 SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 1603
            SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD
Sbjct: 677  SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 736

Query: 1604 DFNKHGPGITQPSV-SPSAPPVASSTPPAPTITSP-TVGLMASREDVLTRAAALGRGAAT 1663
            DFNKHGPGITQPSV SPSAPPV SSTPPAPT+TSP TVGLMASREDVLTRAAALGRGAAT
Sbjct: 737  DFNKHGPGITQPSVSSPSAPPVVSSTPPAPTVTSPSTVGLMASREDVLTRAAALGRGAAT 796

Query: 1664 TGFKRFLALTEAAKDRKDGPFRKLFNP 1689
            TGFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 797  TGFKRFLALTEAAKDRKDGPFRKLFNP 823

BLAST of Lag0037683 vs. NCBI nr
Match: XP_004149523.1 (vacuolar protein sorting-associated protein 53 A [Cucumis sativus] >KGN55451.2 hypothetical protein Csa_012355 [Cucumis sativus])

HSP 1 Score: 1511.5 bits (3912), Expect = 0.0e+00
Identity = 794/807 (98.39%), Postives = 799/807 (99.01%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQS+SGTKAKEDLAAATSAVEELMSKIREI
Sbjct: 17   EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSNSGTKAKEDLAAATSAVEELMSKIREI 76

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA
Sbjct: 77   KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 136

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 1063
            AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL
Sbjct: 137  AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 196

Query: 1064 QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 1123
            QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR
Sbjct: 197  QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 256

Query: 1124 TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFEDE 1183
            TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDI+DNLKEKPDVATLLLALQRTLEFEDE
Sbjct: 257  TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDVATLLLALQRTLEFEDE 316

Query: 1184 LAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKDGIKDMS 1243
            LAEKFGGG RGKE+GN IEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEK+GIKDMS
Sbjct: 317  LAEKFGGGARGKESGNGIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKNGIKDMS 376

Query: 1244 VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 1303
            VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ
Sbjct: 377  VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 436

Query: 1304 LFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQ 1363
            LFLIIKRSLKRCSALTKNQTL NLFKVFQRVLKAYA KLFARLPKGGTGFVAAATGMDGQ
Sbjct: 437  LFLIIKRSLKRCSALTKNQTLLNLFKVFQRVLKAYATKLFARLPKGGTGFVAAATGMDGQ 496

Query: 1364 IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA 1423
            IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA
Sbjct: 497  IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA 556

Query: 1424 LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 1483
            LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF
Sbjct: 557  LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 616

Query: 1484 QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSSAASY 1543
            QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTS AASY
Sbjct: 617  QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSGAASY 676

Query: 1544 SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 1603
            SKFVSREMSKAEALLKVILSP+DSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD
Sbjct: 677  SKFVSREMSKAEALLKVILSPVDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 736

Query: 1604 DFNKHGPGITQPSV-SPSAPPVASSTPPAPTITSP-TVGLMASREDVLTRAAALGRGAAT 1663
            DFNKHGPGITQPSV SPSAPPV SSTPPAPTITSP TVGLMASREDVLTRAAALGRGAAT
Sbjct: 737  DFNKHGPGITQPSVSSPSAPPVVSSTPPAPTITSPSTVGLMASREDVLTRAAALGRGAAT 796

Query: 1664 TGFKRFLALTEAAKDRKDGPFRKLFNP 1689
            TGFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 797  TGFKRFLALTEAAKDRKDGPFRKLFNP 823

BLAST of Lag0037683 vs. NCBI nr
Match: XP_008464655.1 (PREDICTED: vacuolar protein sorting-associated protein 53 A isoform X1 [Cucumis melo])

HSP 1 Score: 1510.7 bits (3910), Expect = 0.0e+00
Identity = 793/807 (98.27%), Postives = 798/807 (98.88%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI
Sbjct: 17   EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 76

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA
Sbjct: 77   KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 136

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 1063
            AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL
Sbjct: 137  AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 196

Query: 1064 QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 1123
            QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR
Sbjct: 197  QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 256

Query: 1124 TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFEDE 1183
            TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDI+DNLKEKPDVATLLLALQRTLEFEDE
Sbjct: 257  TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDVATLLLALQRTLEFEDE 316

Query: 1184 LAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKDGIKDMS 1243
            LAEKFGGG RGKE+GN IEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEK+GIKDMS
Sbjct: 317  LAEKFGGGARGKESGNGIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKNGIKDMS 376

Query: 1244 VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 1303
            VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ
Sbjct: 377  VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 436

Query: 1304 LFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQ 1363
            LFLIIKRSLKRCSALTKNQTL NLFKVFQRVLKAYA KLFARLPKGGTGFVAAATGMDGQ
Sbjct: 437  LFLIIKRSLKRCSALTKNQTLLNLFKVFQRVLKAYATKLFARLPKGGTGFVAAATGMDGQ 496

Query: 1364 IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA 1423
            IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA
Sbjct: 497  IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA 556

Query: 1424 LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 1483
            LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPV GRLLSPLYF
Sbjct: 557  LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVFGRLLSPLYF 616

Query: 1484 QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSSAASY 1543
            QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTS AASY
Sbjct: 617  QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSGAASY 676

Query: 1544 SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 1603
            SKFVSREMSKAEALLKVILSP+DSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD
Sbjct: 677  SKFVSREMSKAEALLKVILSPVDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 736

Query: 1604 DFNKHGPGITQPSV-SPSAPPVASSTPPAPTITSP-TVGLMASREDVLTRAAALGRGAAT 1663
            DFNKHGPGITQPSV SPSAPPV SSTPPAPT+TSP TVGLMASREDVLTRAAALGRGAAT
Sbjct: 737  DFNKHGPGITQPSVSSPSAPPVVSSTPPAPTVTSPSTVGLMASREDVLTRAAALGRGAAT 796

Query: 1664 TGFKRFLALTEAAKDRKDGPFRKLFNP 1689
            TGFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 797  TGFKRFLALTEAAKDRKDGPFRKLFNP 823

BLAST of Lag0037683 vs. NCBI nr
Match: XP_023535153.1 (vacuolar protein sorting-associated protein 53 A [Cucurbita pepo subsp. pepo] >KAG6591821.1 Vacuolar protein sorting-associated protein 53 A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024687.1 Vacuolar protein sorting-associated protein 53 A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1509.6 bits (3907), Expect = 0.0e+00
Identity = 787/805 (97.76%), Postives = 796/805 (98.88%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI
Sbjct: 17   EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 76

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA
Sbjct: 77   KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 136

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 1063
            AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL
Sbjct: 137  AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 196

Query: 1064 QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 1123
            QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRR+R
Sbjct: 197  QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRMR 256

Query: 1124 TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFEDE 1183
            TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDI+DNLKEKPDVATLLLALQRTLEFEDE
Sbjct: 257  TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDVATLLLALQRTLEFEDE 316

Query: 1184 LAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKDGIKDMS 1243
            LAEKFGGG +GKEN NEIEEFGRED+NSQNVSDIRKKYEKKLAVHQGPE DEKDGIK+MS
Sbjct: 317  LAEKFGGGAQGKENRNEIEEFGREDNNSQNVSDIRKKYEKKLAVHQGPETDEKDGIKEMS 376

Query: 1244 VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 1303
            VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ
Sbjct: 377  VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 436

Query: 1304 LFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQ 1363
            LFLIIKRSLKRCSALTKNQTL NLFKVFQRVLKAYA KL ARLPKGG+GFVAAATGMDGQ
Sbjct: 437  LFLIIKRSLKRCSALTKNQTLLNLFKVFQRVLKAYATKLLARLPKGGSGFVAAATGMDGQ 496

Query: 1364 IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA 1423
            IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIID+QLVDGVDMSEVQDEFSAVITKA
Sbjct: 497  IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDTQLVDGVDMSEVQDEFSAVITKA 556

Query: 1424 LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 1483
            LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF
Sbjct: 557  LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 616

Query: 1484 QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSSAASY 1543
            QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTS AASY
Sbjct: 617  QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSGAASY 676

Query: 1544 SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 1603
            SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD
Sbjct: 677  SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 736

Query: 1604 DFNKHGPGITQPSVSPSAPPVASSTPPAPTITSPTVGLMASREDVLTRAAALGRGAATTG 1663
            DFNKHGPGITQPSVSPS PP+AS+ PPAPTITSPTVGLMASREDVLTRAAALGRGAATTG
Sbjct: 737  DFNKHGPGITQPSVSPSVPPIASAAPPAPTITSPTVGLMASREDVLTRAAALGRGAATTG 796

Query: 1664 FKRFLALTEAAKDRKDGPFRKLFNP 1689
            FKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 797  FKRFLALTEAAKDRKDGPFRKLFNP 821

BLAST of Lag0037683 vs. NCBI nr
Match: XP_022936047.1 (vacuolar protein sorting-associated protein 53 A [Cucurbita moschata] >XP_022936048.1 vacuolar protein sorting-associated protein 53 A [Cucurbita moschata] >XP_022936049.1 vacuolar protein sorting-associated protein 53 A [Cucurbita moschata] >XP_022936050.1 vacuolar protein sorting-associated protein 53 A [Cucurbita moschata] >XP_022976701.1 vacuolar protein sorting-associated protein 53 A [Cucurbita maxima] >XP_022976702.1 vacuolar protein sorting-associated protein 53 A [Cucurbita maxima] >XP_022976703.1 vacuolar protein sorting-associated protein 53 A [Cucurbita maxima])

HSP 1 Score: 1508.4 bits (3904), Expect = 0.0e+00
Identity = 786/805 (97.64%), Postives = 796/805 (98.88%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI
Sbjct: 17   EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 76

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA
Sbjct: 77   KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 136

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 1063
            AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL
Sbjct: 137  AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 196

Query: 1064 QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 1123
            QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRR+R
Sbjct: 197  QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRMR 256

Query: 1124 TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFEDE 1183
            TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDI+DNLKEKPDVATLLLALQRTLEFEDE
Sbjct: 257  TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDVATLLLALQRTLEFEDE 316

Query: 1184 LAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKDGIKDMS 1243
            LAEKFGGG +GKEN NEIEEFGRED+NSQNVSDIRKKYEKKLAVHQGPE DEKDGIK+MS
Sbjct: 317  LAEKFGGGAQGKENRNEIEEFGREDNNSQNVSDIRKKYEKKLAVHQGPETDEKDGIKEMS 376

Query: 1244 VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 1303
            VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ
Sbjct: 377  VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 436

Query: 1304 LFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQ 1363
            LFLIIKRSLKRCSALTKNQTL NLFKVFQRVLKAYA KL ARLPKGG+GFVAAATGMDGQ
Sbjct: 437  LFLIIKRSLKRCSALTKNQTLLNLFKVFQRVLKAYATKLLARLPKGGSGFVAAATGMDGQ 496

Query: 1364 IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA 1423
            IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIID+QLVDGVDMSEVQDEFSAVITKA
Sbjct: 497  IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDTQLVDGVDMSEVQDEFSAVITKA 556

Query: 1424 LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 1483
            LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF
Sbjct: 557  LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 616

Query: 1484 QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSSAASY 1543
            QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTS AASY
Sbjct: 617  QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSGAASY 676

Query: 1544 SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 1603
            SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD
Sbjct: 677  SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 736

Query: 1604 DFNKHGPGITQPSVSPSAPPVASSTPPAPTITSPTVGLMASREDVLTRAAALGRGAATTG 1663
            DFNKHGPGITQPSVSPS PP+AS+ PPAPTITSPTVG+MASREDVLTRAAALGRGAATTG
Sbjct: 737  DFNKHGPGITQPSVSPSVPPIASAAPPAPTITSPTVGVMASREDVLTRAAALGRGAATTG 796

Query: 1664 FKRFLALTEAAKDRKDGPFRKLFNP 1689
            FKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 797  FKRFLALTEAAKDRKDGPFRKLFNP 821

BLAST of Lag0037683 vs. ExPASy Swiss-Prot
Match: Q0WQF4 (Vacuolar protein sorting-associated protein 53 A OS=Arabidopsis thaliana OX=3702 GN=VPS53 PE=1 SV=1)

HSP 1 Score: 1256.5 bits (3250), Expect = 0.0e+00
Identity = 665/812 (81.90%), Postives = 727/812 (89.53%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            EASL+GVEPLMQKI  EIRRVDA IL+AVRQQS+SGTKAKEDLA AT AVEEL  KI+EI
Sbjct: 17   EASLTGVEPLMQKIQGEIRRVDASILSAVRQQSNSGTKAKEDLADATRAVEELSHKIQEI 76

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            K+KAEQSE MVQEICRDIKKLDFAKK+ITTTITALHRLTMLVSAVEQLQVMASKRQYKEA
Sbjct: 77   KSKAEQSEAMVQEICRDIKKLDFAKKNITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 136

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 1063
            AAQLEAVNQLC+HFEAYRD PKITELREK  NIKQILKSHVFSDFSSLGTGKE EETNLL
Sbjct: 137  AAQLEAVNQLCNHFEAYRDVPKITELREKLNNIKQILKSHVFSDFSSLGTGKETEETNLL 196

Query: 1064 QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 1123
            Q+LSD+C VVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR
Sbjct: 197  QKLSDSCLVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 256

Query: 1124 TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFEDE 1183
            TNEEIWKIFP SWHVPYRLCIQFCK+TRKQ+E I+ N+KEKP VA LLLALQ T+EFE E
Sbjct: 257  TNEEIWKIFPASWHVPYRLCIQFCKQTRKQVESILVNMKEKPVVAILLLALQSTVEFEKE 316

Query: 1184 LAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPEND----EKDGI 1243
            L +KFGGG   K+  ++IEE G  + NSQN+S IRKKYEKK A  Q  E +    EK G 
Sbjct: 317  LEKKFGGGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKFAASQETEENGFQQEKTGN 376

Query: 1244 KDMSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLS 1303
            KD+SV GAGFNFRG++SSCFEPHLT YIELEEKTLM++LEK+VQEE+WD+++GSQ+NVLS
Sbjct: 377  KDLSVTGAGFNFRGMISSCFEPHLTPYIELEEKTLMDDLEKIVQEESWDVEDGSQNNVLS 436

Query: 1304 SSMQLFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATG 1363
            SS QLF  IK+SLKRC+ L+KNQTLFNLFKVFQRVLKAYA KLF +LPKGGTG VAAATG
Sbjct: 437  SSTQLFSNIKKSLKRCNTLSKNQTLFNLFKVFQRVLKAYATKLFFKLPKGGTGIVAAATG 496

Query: 1364 MDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAV 1423
            MDGQIK S++DE+VICYIVNSAEYCHKTSGELAE+V +IID    DGVDMSEVQDEFSAV
Sbjct: 497  MDGQIKVSERDERVICYIVNSAEYCHKTSGELAENVSEIIDPHYADGVDMSEVQDEFSAV 556

Query: 1424 ITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLS 1483
            ITKALVTLV GLETKFD+EMA MTRVPW TLESVGDQS YVNGIN +L+ SIPVLG+LL+
Sbjct: 557  ITKALVTLVLGLETKFDTEMAVMTRVPWSTLESVGDQSGYVNGINTVLSGSIPVLGKLLT 616

Query: 1484 PLYFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSS 1543
            P+YFQFFLDKLASSLGPRFYANIF+CKQ+SETGAQQMLLDTQAVK+ILL+IPSL RQTS+
Sbjct: 617  PVYFQFFLDKLASSLGPRFYANIFRCKQLSETGAQQMLLDTQAVKSILLEIPSLARQTST 676

Query: 1544 AASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQ 1603
            AASYSKFVSREMS+AEALLKVILSPIDSVADTYRAL PEGTPMEFQRILELKG KKADQQ
Sbjct: 677  AASYSKFVSREMSRAEALLKVILSPIDSVADTYRALFPEGTPMEFQRILELKGLKKADQQ 736

Query: 1604 SILDDFNKHGPGITQPSVSPSAPPVASSTPPAP--TITSP--TVGLMASREDVLTRAAAL 1663
            SILDDFNKHGPG TQ SV+ +A P    TPPAP   IT+P    G +A+ EDVLTRAAAL
Sbjct: 737  SILDDFNKHGPGFTQQSVA-AAMPQPMPTPPAPPLAITNPATAAGFIANSEDVLTRAAAL 796

Query: 1664 GRGAATTGFKRFLALTEAAKDRKDGPFRKLFN 1688
            GRGAA+TGFK+F+ALTEAAKDRKDGP R+LFN
Sbjct: 797  GRGAASTGFKKFIALTEAAKDRKDGPLRRLFN 827

BLAST of Lag0037683 vs. ExPASy Swiss-Prot
Match: F4I7Y2 (Vacuolar protein sorting-associated protein 53 B OS=Arabidopsis thaliana OX=3702 GN=At1g50970 PE=2 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 7.4e-149
Identity = 368/814 (45.21%), Postives = 422/814 (51.84%), Query Frame = 0

Query: 883  LEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIRE 942
            +EASLS VE  MQKI +EIRRVDA ILA V QQ +SGT+AKE+L  A  A EEL  KI+E
Sbjct: 16   VEASLSCVESFMQKICDEIRRVDATILAVVSQQGNSGTRAKENLNDAICAAEELSHKIQE 75

Query: 943  IKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKE 1002
            IK+KAEQ+E MVQ+IC DIKKLDFAKK+ITT +TAL RLTMLVSAV+QLQVM SKRQYKE
Sbjct: 76   IKSKAEQTEAMVQDICSDIKKLDFAKKNITTAVTALSRLTMLVSAVQQLQVMTSKRQYKE 135

Query: 1003 AAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNL 1062
            AA QLEA+N+LC+HF+AY D PKI ELREK KNIKQILK HVFSDFSSLGTG E EE  L
Sbjct: 136  AATQLEAINELCNHFKAYMDLPKIMELREKLKNIKQILKFHVFSDFSSLGTGTETEELFL 195

Query: 1063 LQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRI 1122
            L++LSD+C VVDALEPSVREEL+NNFCSRELTSYEQI+ GAEL  LD+ E  Y  +   I
Sbjct: 196  LKKLSDSCLVVDALEPSVREELINNFCSRELTSYEQIYVGAELKTLDEIELIYNQLSCLI 255

Query: 1123 RTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFED 1182
            R N+  W IFP SWHVPYRLCIQ  +KTR Q+E I+ NLKEK DV  LLL L+RTLEFE 
Sbjct: 256  RKNQGKWTIFPASWHVPYRLCIQLSRKTRVQVESILVNLKEKSDVEKLLLELKRTLEFER 315

Query: 1183 ELAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKDGIKDM 1242
            EL  KFGG   G   G++I   G   +NSQ                              
Sbjct: 316  ELEMKFGG---GGSIGDDI--IGGGGNNSQK----------------------------- 375

Query: 1243 SVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSM 1302
                  FNFRG++SSCFEPHLT+YIE EE  LM+ LEK+VQEETWDI+E           
Sbjct: 376  ------FNFRGMISSCFEPHLTIYIEKEEMELMQLLEKVVQEETWDIEEELG-------- 435

Query: 1303 QLFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDG 1362
                                                                        
Sbjct: 436  ------------------------------------------------------------ 495

Query: 1363 QIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITK 1422
                                 CH                                     
Sbjct: 496  ---------------------CH------------------------------------- 555

Query: 1423 ALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLY 1482
                                                                        
Sbjct: 556  ------------------------------------------------------------ 569

Query: 1483 FQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQ------ 1542
                                      SE     MLLD   +K ILL +PSL RQ      
Sbjct: 616  --------------------------SENSVYLMLLDAHDMKMILLKVPSLARQPEASAL 569

Query: 1543 --TSSAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFK 1602
               ++ ASY K V+ +M +AEA+LKVI SPI +V DTYRAL PE TPMEFQRIL LKG  
Sbjct: 676  LVKTATASYVKLVNHQMKRAEAVLKVIASPIVTVIDTYRALFPEETPMEFQRILVLKGLT 569

Query: 1603 KADQQSILDDFNKHGPGITQPSVSPSAPPVASSTPPAPTITSPTVGLMASREDVLTRAAA 1662
            KA+QQSILDDFN H   ITQ SV+   P  A + P A T  +P V   A+ E+VLTRAA+
Sbjct: 736  KAEQQSILDDFNNHSSRITQLSVAAKTPE-AHALPLALTNVAPAVRFKANSEEVLTRAAS 569

Query: 1663 LGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 1689
                AATT F +  ALT AAKDR   PFRKLFNP
Sbjct: 796  ----AATTSFMKLYALTGAAKDR---PFRKLFNP 569

BLAST of Lag0037683 vs. ExPASy Swiss-Prot
Match: Q5ZLD7 (Vacuolar protein sorting-associated protein 53 homolog OS=Gallus gallus OX=9031 GN=VPS53 PE=2 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 2.0e-114
Identity = 275/789 (34.85%), Postives = 449/789 (56.91%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            E SL+ ++ ++ KI  +IR++D  I   VR Q++ G   ++ L  A  A+++L  KI++I
Sbjct: 54   EQSLANIDEVVNKIRLKIRKLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDI 113

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            K KAE+SE MV+EI RDIK+LD AK+H+TT+IT L+ L ML   V+ L+ M  +RQY E 
Sbjct: 114  KDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEV 173

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFS----SLGTGKEKEE 1063
            A  L+ V  +  HF  Y   P+I +L E+ K  +  L   + +DF     S GT +    
Sbjct: 174  ANLLQGVVNVLEHFNKYMGIPQIRQLSERVKAAQNELGQQILADFEEAFPSQGTKRPGGP 233

Query: 1064 TNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIF-EGAELAKLDKTERRYAWI 1123
            +N+L+   DAC V + L+P +++E++  F  + L+ Y  +F E  ++A LDK +RRYAWI
Sbjct: 234  SNVLR---DACLVANVLDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWI 293

Query: 1124 KRRIRTNEEIW-KIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRT 1183
            KR++   EE + ++FP  W +  R+ ++FC  TR +L  IM    ++ +V  LL A+QRT
Sbjct: 294  KRQLVDYEEKYGRMFPQEWCMTERIAVEFCHVTRTELAKIMRTRAKEIEVKLLLFAIQRT 353

Query: 1184 LEFEDELAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKD 1243
              FE  LA++F G T    +G   +      S +  + D       ++ +    E  + D
Sbjct: 354  TNFEGLLAKRFSGCTLA--DGTVKKPEAPPPSTNPFLEDETGTETDEIVI----EKSDAD 413

Query: 1244 GIKDMSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQE------ETWDIDE 1303
              K   VP   F+  GIVS CFEPHL VYIE ++K L E +++ V +         ++DE
Sbjct: 414  KPKKPKVPDNPFH--GIVSKCFEPHLYVYIESQDKNLSELIDRFVADFKAQGPPKPNVDE 473

Query: 1304 GSQSNVLSSSMQLFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFA-RLPK-- 1363
            G    VL S   LF+  K+ + +CS L+  + +  L  +FQ+ L+ YA K+ +  LPK  
Sbjct: 474  GGA--VLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTT 533

Query: 1364 ---GGTGFVAAATGMDGQ--IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQL 1423
               GG    +     +G    K + ++  +IC I+++AEYC  T+ +L E +++ +D+ L
Sbjct: 534  SSSGGLTITSLLKEKEGSEVAKFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDASL 593

Query: 1424 VDGVDMSEVQDEFSAVITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGI 1483
            V+ ++++   D FS VI+ ++  LV  L+   D  + AM+++ W  +E VGDQS YV  +
Sbjct: 594  VERINLTGETDTFSIVISNSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSV 653

Query: 1484 NMILTTSIPVLGRLLSPL--YFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQ 1543
             + +  ++P++   L+    YF  F  K A+S  P+F  ++FKCK IS  GA+Q+LLDT 
Sbjct: 654  ILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFINHLFKCKPISMVGAEQLLLDTH 713

Query: 1544 AVKTILLDIPSLGRQT--SSAASYSKFVSREMSKAEALLKVILSPIDS---VADTYRALL 1603
            ++K +LLD+PS+G Q    + ASY++ V + M++AE +LKV+++P +      D Y  LL
Sbjct: 714  SLKMVLLDLPSIGSQVVRKAPASYTRIVVKGMTRAEMILKVVMAPHEPPVVFVDNYIKLL 773

Query: 1604 PEGTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSAPPVASSTPPAPTITS 1646
             + +   FQ+IL++KG K+++Q S+L+ F +  P          APP       + ++++
Sbjct: 774  ADCSTDTFQKILDMKGLKRSEQSSMLELFRQRLP----------APPSGVENSGSLSLSA 819

BLAST of Lag0037683 vs. ExPASy Swiss-Prot
Match: Q8CCB4 (Vacuolar protein sorting-associated protein 53 homolog OS=Mus musculus OX=10090 GN=Vps53 PE=1 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 5.9e-114
Identity = 276/790 (34.94%), Postives = 447/790 (56.58%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            E SL+ ++ ++ KI  +IRR+D  I   VR Q++ G   ++ L  A  A+++L  KI++I
Sbjct: 55   EQSLANIDDVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDI 114

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            K KAE+SE MV+EI RDIK+LD AK+H+TT+IT L+ L ML   V+ L+ M  +RQY E 
Sbjct: 115  KDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEV 174

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFS----SLGTGKEKEE 1063
            A  L+ V  +  HF  Y   P+I +L E+ K  +  L   + +DF     S GT +    
Sbjct: 175  ANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGP 234

Query: 1064 TNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIF-EGAELAKLDKTERRYAWI 1123
            +N+L+   DAC V + L+P +++E++  F  + L+ Y  +F E  ++A LDK +RRYAW+
Sbjct: 235  SNVLR---DACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWV 294

Query: 1124 KRRIRTNEEIW-KIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRT 1183
            KR++   EE + ++FP  W +  R+ ++FC  TR +L  IM    ++ +V  LL A+QRT
Sbjct: 295  KRQLVDYEEKYGRMFPREWCMTERISVEFCHVTRAELSKIMRARAKEIEVKLLLFAIQRT 354

Query: 1184 LEFEDELAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKD 1243
              FE  LA++F G T       ++E      S +  + D      ++LA+       EK 
Sbjct: 355  TNFEGFLAKRFSGCTLTDGTLKKLE--SPPPSTNPFLEDETTPEMEELAL-------EKG 414

Query: 1244 GIKDMSVPGAGFN-FRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQE------ETWDID 1303
             ++    P A  N F GIVS CFEPHL VYIE ++K L E +++ V +         + D
Sbjct: 415  ELEQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLSELIDRFVADFKAQGPPKPNTD 474

Query: 1304 EGSQSNVLSSSMQLFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFA-RLPK- 1363
            EG    VL S   LF+  K+ + +CS L+  + +  L  +FQ+ L+ YA K+ +  LPK 
Sbjct: 475  EGGA--VLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKT 534

Query: 1364 ----GGTGFVAAATGMDGQ--IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQ 1423
                GG    +     +G    + + ++  +IC I+++AEYC  T+ +L E +++ +D  
Sbjct: 535  SSSSGGLTISSLLKEKEGSEVARFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDVS 594

Query: 1424 LVDGVDMSEVQDEFSAVITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNG 1483
            L + ++++   D FS VI+ ++  LV  L+   D  + AM+++PW  +E VGDQS YV  
Sbjct: 595  LTERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALIAMSKMPWQNVEHVGDQSPYVTS 654

Query: 1484 INMILTTSIPVLGRLLSPL--YFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDT 1543
            + + +  ++P++   L+    YF  F  K A+S  P+F  ++FKCK IS  GA+Q+LLDT
Sbjct: 655  VILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFITHLFKCKPISMVGAEQLLLDT 714

Query: 1544 QAVKTILLDIPSLGRQT--SSAASYSKFVSREMSKAEALLKVILSPIDSV---ADTYRAL 1603
             ++K +LLD+PS+G Q    + ASY+K V + M++AE +LKV+++P + +    D Y  L
Sbjct: 715  HSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIKL 774

Query: 1604 LPEGTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSAPPVASSTPPAPTIT 1646
            L +     FQ+IL++KG K+++Q S+L+   +  P          APP  +      ++ 
Sbjct: 775  LTDCNSETFQKILDMKGLKRSEQSSMLELLRQRLP----------APPSGTEGSSTLSLI 820

BLAST of Lag0037683 vs. ExPASy Swiss-Prot
Match: Q5VIR6 (Vacuolar protein sorting-associated protein 53 homolog OS=Homo sapiens OX=9606 GN=VPS53 PE=1 SV=2)

HSP 1 Score: 414.1 bits (1063), Expect = 7.7e-114
Identity = 277/790 (35.06%), Postives = 448/790 (56.71%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            E SL+ ++ ++ KI  +IRR+D  I   VR Q++ G   ++ L  A  A+++L  KI++I
Sbjct: 55   EQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDI 114

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            K KAE+SE MV+EI RDIK+LD AK+H+TT+IT L+ L ML   V+ L+ M  +RQY E 
Sbjct: 115  KDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEV 174

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFS----SLGTGKEKEE 1063
            A  L+ V  +  HF  Y   P+I +L E+ K  +  L   + +DF     S GT +    
Sbjct: 175  ANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILADFEEAFPSQGTKRPGGP 234

Query: 1064 TNLLQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIF-EGAELAKLDKTERRYAWI 1123
            +N+L+   DAC V + L+P +++E++  F  + L+ Y  +F E  ++A LDK +RRYAWI
Sbjct: 235  SNVLR---DACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWI 294

Query: 1124 KRRIRTNEEIW-KIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRT 1183
            KR++   EE + ++FP  W +  R+ ++FC  TR +L  IM    ++ +V  LL A+QRT
Sbjct: 295  KRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQRT 354

Query: 1184 LEFEDELAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKD 1243
              FE  LA++F G T       ++E      S +  + D      ++LA  +G  +  K 
Sbjct: 355  TNFEGFLAKRFSGCTLTDGTLKKLE--SPPPSTNPFLEDEPTPEMEELATEKGDLDQPKK 414

Query: 1244 GIKDMSVPGAGFN-FRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQE------ETWDID 1303
                   P A  N F GIVS CFEPHL VYIE ++K L E +++ V +         + D
Sbjct: 415  -------PKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTD 474

Query: 1304 EGSQSNVLSSSMQLFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFA-RLPK- 1363
            EG    VL S   LF+  K+ + +CS L+  + +  L  +FQ+ L+ YA K+ +  LPK 
Sbjct: 475  EGGA--VLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKT 534

Query: 1364 ----GGTGFVAAATGMDGQ--IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQ 1423
                GG    +     +G    K + ++  +IC I+++AEYC  T+ +L E +++ +D  
Sbjct: 535  TTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATTQQLEEKLKEKVDVS 594

Query: 1424 LVDGVDMSEVQDEFSAVITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNG 1483
            L++ ++++   D FS VI+ ++  LV  L+   D  + AM+++ W  +E VGDQS YV  
Sbjct: 595  LIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTS 654

Query: 1484 INMILTTSIPVLGRLLSPL--YFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDT 1543
            + + +  ++P++   L+    YF  F  K A+S  P+F  ++FKCK IS  GA+Q+LLDT
Sbjct: 655  VILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQLLLDT 714

Query: 1544 QAVKTILLDIPSLGRQT--SSAASYSKFVSREMSKAEALLKVILSPIDSV---ADTYRAL 1603
             ++K +LLD+PS+  Q    + ASY+K V + M++AE +LKV+++P + +    D Y  L
Sbjct: 715  HSLKMVLLDLPSISSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIKL 774

Query: 1604 LPEGTPMEFQRILELKGFKKADQQSILDDFNKHGPGITQPSVSPSAPPVASSTPPAPTIT 1646
            L +     FQ+IL++KG K+++Q S+L+   +  P          APP  + +  + ++T
Sbjct: 775  LTDCNTETFQKILDMKGLKRSEQSSMLELLRQRLP----------APPSGAESSGSLSLT 820

BLAST of Lag0037683 vs. ExPASy TrEMBL
Match: A0A1S3CNJ3 (vacuolar protein sorting-associated protein 53 A isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502491 PE=3 SV=1)

HSP 1 Score: 1510.7 bits (3910), Expect = 0.0e+00
Identity = 793/807 (98.27%), Postives = 798/807 (98.88%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI
Sbjct: 17   EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 76

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA
Sbjct: 77   KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 136

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 1063
            AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL
Sbjct: 137  AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 196

Query: 1064 QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 1123
            QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR
Sbjct: 197  QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 256

Query: 1124 TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFEDE 1183
            TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDI+DNLKEKPDVATLLLALQRTLEFEDE
Sbjct: 257  TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDVATLLLALQRTLEFEDE 316

Query: 1184 LAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKDGIKDMS 1243
            LAEKFGGG RGKE+GN IEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEK+GIKDMS
Sbjct: 317  LAEKFGGGARGKESGNGIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKNGIKDMS 376

Query: 1244 VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 1303
            VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ
Sbjct: 377  VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 436

Query: 1304 LFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQ 1363
            LFLIIKRSLKRCSALTKNQTL NLFKVFQRVLKAYA KLFARLPKGGTGFVAAATGMDGQ
Sbjct: 437  LFLIIKRSLKRCSALTKNQTLLNLFKVFQRVLKAYATKLFARLPKGGTGFVAAATGMDGQ 496

Query: 1364 IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA 1423
            IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA
Sbjct: 497  IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA 556

Query: 1424 LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 1483
            LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPV GRLLSPLYF
Sbjct: 557  LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVFGRLLSPLYF 616

Query: 1484 QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSSAASY 1543
            QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTS AASY
Sbjct: 617  QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSGAASY 676

Query: 1544 SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 1603
            SKFVSREMSKAEALLKVILSP+DSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD
Sbjct: 677  SKFVSREMSKAEALLKVILSPVDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 736

Query: 1604 DFNKHGPGITQPSV-SPSAPPVASSTPPAPTITSP-TVGLMASREDVLTRAAALGRGAAT 1663
            DFNKHGPGITQPSV SPSAPPV SSTPPAPT+TSP TVGLMASREDVLTRAAALGRGAAT
Sbjct: 737  DFNKHGPGITQPSVSSPSAPPVVSSTPPAPTVTSPSTVGLMASREDVLTRAAALGRGAAT 796

Query: 1664 TGFKRFLALTEAAKDRKDGPFRKLFNP 1689
            TGFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 797  TGFKRFLALTEAAKDRKDGPFRKLFNP 823

BLAST of Lag0037683 vs. ExPASy TrEMBL
Match: A0A6J1IHL8 (vacuolar protein sorting-associated protein 53 A OS=Cucurbita maxima OX=3661 GN=LOC111477013 PE=3 SV=1)

HSP 1 Score: 1508.4 bits (3904), Expect = 0.0e+00
Identity = 786/805 (97.64%), Postives = 796/805 (98.88%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI
Sbjct: 17   EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 76

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA
Sbjct: 77   KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 136

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 1063
            AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL
Sbjct: 137  AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 196

Query: 1064 QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 1123
            QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRR+R
Sbjct: 197  QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRMR 256

Query: 1124 TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFEDE 1183
            TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDI+DNLKEKPDVATLLLALQRTLEFEDE
Sbjct: 257  TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDVATLLLALQRTLEFEDE 316

Query: 1184 LAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKDGIKDMS 1243
            LAEKFGGG +GKEN NEIEEFGRED+NSQNVSDIRKKYEKKLAVHQGPE DEKDGIK+MS
Sbjct: 317  LAEKFGGGAQGKENRNEIEEFGREDNNSQNVSDIRKKYEKKLAVHQGPETDEKDGIKEMS 376

Query: 1244 VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 1303
            VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ
Sbjct: 377  VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 436

Query: 1304 LFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQ 1363
            LFLIIKRSLKRCSALTKNQTL NLFKVFQRVLKAYA KL ARLPKGG+GFVAAATGMDGQ
Sbjct: 437  LFLIIKRSLKRCSALTKNQTLLNLFKVFQRVLKAYATKLLARLPKGGSGFVAAATGMDGQ 496

Query: 1364 IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA 1423
            IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIID+QLVDGVDMSEVQDEFSAVITKA
Sbjct: 497  IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDTQLVDGVDMSEVQDEFSAVITKA 556

Query: 1424 LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 1483
            LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF
Sbjct: 557  LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 616

Query: 1484 QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSSAASY 1543
            QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTS AASY
Sbjct: 617  QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSGAASY 676

Query: 1544 SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 1603
            SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD
Sbjct: 677  SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 736

Query: 1604 DFNKHGPGITQPSVSPSAPPVASSTPPAPTITSPTVGLMASREDVLTRAAALGRGAATTG 1663
            DFNKHGPGITQPSVSPS PP+AS+ PPAPTITSPTVG+MASREDVLTRAAALGRGAATTG
Sbjct: 737  DFNKHGPGITQPSVSPSVPPIASAAPPAPTITSPTVGVMASREDVLTRAAALGRGAATTG 796

Query: 1664 FKRFLALTEAAKDRKDGPFRKLFNP 1689
            FKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 797  FKRFLALTEAAKDRKDGPFRKLFNP 821

BLAST of Lag0037683 vs. ExPASy TrEMBL
Match: A0A6J1F767 (vacuolar protein sorting-associated protein 53 A OS=Cucurbita moschata OX=3662 GN=LOC111442766 PE=3 SV=1)

HSP 1 Score: 1508.4 bits (3904), Expect = 0.0e+00
Identity = 786/805 (97.64%), Postives = 796/805 (98.88%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI
Sbjct: 17   EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 76

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA
Sbjct: 77   KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 136

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 1063
            AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL
Sbjct: 137  AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 196

Query: 1064 QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 1123
            QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRR+R
Sbjct: 197  QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRMR 256

Query: 1124 TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFEDE 1183
            TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDI+DNLKEKPDVATLLLALQRTLEFEDE
Sbjct: 257  TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDVATLLLALQRTLEFEDE 316

Query: 1184 LAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKDGIKDMS 1243
            LAEKFGGG +GKEN NEIEEFGRED+NSQNVSDIRKKYEKKLAVHQGPE DEKDGIK+MS
Sbjct: 317  LAEKFGGGAQGKENRNEIEEFGREDNNSQNVSDIRKKYEKKLAVHQGPETDEKDGIKEMS 376

Query: 1244 VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 1303
            VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ
Sbjct: 377  VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 436

Query: 1304 LFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQ 1363
            LFLIIKRSLKRCSALTKNQTL NLFKVFQRVLKAYA KL ARLPKGG+GFVAAATGMDGQ
Sbjct: 437  LFLIIKRSLKRCSALTKNQTLLNLFKVFQRVLKAYATKLLARLPKGGSGFVAAATGMDGQ 496

Query: 1364 IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA 1423
            IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIID+QLVDGVDMSEVQDEFSAVITKA
Sbjct: 497  IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDTQLVDGVDMSEVQDEFSAVITKA 556

Query: 1424 LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 1483
            LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF
Sbjct: 557  LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 616

Query: 1484 QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSSAASY 1543
            QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTS AASY
Sbjct: 617  QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSGAASY 676

Query: 1544 SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 1603
            SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD
Sbjct: 677  SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 736

Query: 1604 DFNKHGPGITQPSVSPSAPPVASSTPPAPTITSPTVGLMASREDVLTRAAALGRGAATTG 1663
            DFNKHGPGITQPSVSPS PP+AS+ PPAPTITSPTVG+MASREDVLTRAAALGRGAATTG
Sbjct: 737  DFNKHGPGITQPSVSPSVPPIASAAPPAPTITSPTVGVMASREDVLTRAAALGRGAATTG 796

Query: 1664 FKRFLALTEAAKDRKDGPFRKLFNP 1689
            FKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 797  FKRFLALTEAAKDRKDGPFRKLFNP 821

BLAST of Lag0037683 vs. ExPASy TrEMBL
Match: A0A6J1DXM1 (vacuolar protein sorting-associated protein 53 A OS=Momordica charantia OX=3673 GN=LOC111025277 PE=3 SV=1)

HSP 1 Score: 1504.6 bits (3894), Expect = 0.0e+00
Identity = 784/806 (97.27%), Postives = 798/806 (99.01%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI
Sbjct: 17   EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 76

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQ+MASKRQYKEA
Sbjct: 77   KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQLMASKRQYKEA 136

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 1063
            AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL
Sbjct: 137  AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 196

Query: 1064 QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 1123
            QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR
Sbjct: 197  QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 256

Query: 1124 TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFEDE 1183
            TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDV TLLLALQRTLEFEDE
Sbjct: 257  TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVGTLLLALQRTLEFEDE 316

Query: 1184 LAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKDGIKDMS 1243
            LAEKFGGG RGKENGNEIEEFGRED+NSQNVSDIRKKYEKKLAVHQG ENDEKDG+KD+S
Sbjct: 317  LAEKFGGGARGKENGNEIEEFGREDNNSQNVSDIRKKYEKKLAVHQGTENDEKDGVKDVS 376

Query: 1244 VPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQ 1303
            VPGAGFNFRGI+SSCFEPHLTVYIELEEKTLMENLEKLVQEETWDI+EGSQ+NVLSSSMQ
Sbjct: 377  VPGAGFNFRGIISSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIEEGSQNNVLSSSMQ 436

Query: 1304 LFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQ 1363
            LFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQ
Sbjct: 437  LFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQ 496

Query: 1364 IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKA 1423
            IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDG+DMSEVQDEFSAVITKA
Sbjct: 497  IKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGIDMSEVQDEFSAVITKA 556

Query: 1424 LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYF 1483
            LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILT+SIPVLGRLLSPLYF
Sbjct: 557  LVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTSSIPVLGRLLSPLYF 616

Query: 1484 QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSSAASY 1543
            QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTS AASY
Sbjct: 617  QFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSGAASY 676

Query: 1544 SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 1603
            SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD
Sbjct: 677  SKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILD 736

Query: 1604 DFNKHGPGITQPSVSPSAPPVASSTPPAPTITSP-TVGLMASREDVLTRAAALGRGAATT 1663
            DFNKHGPGI QPSVSPSAP + +STPPAPTI+SP +VG+MASREDVLTRAAALGRGAATT
Sbjct: 737  DFNKHGPGIMQPSVSPSAPHIVTSTPPAPTISSPASVGVMASREDVLTRAAALGRGAATT 796

Query: 1664 GFKRFLALTEAAKDRKDGPFRKLFNP 1689
            GFKRFLALTEAAKDRKDGPFRKLFNP
Sbjct: 797  GFKRFLALTEAAKDRKDGPFRKLFNP 822

BLAST of Lag0037683 vs. ExPASy TrEMBL
Match: A0A5D3DXS3 (Vacuolar protein sorting-associated protein 53 A isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G003100 PE=3 SV=1)

HSP 1 Score: 1491.9 bits (3861), Expect = 0.0e+00
Identity = 783/797 (98.24%), Postives = 788/797 (98.87%), Query Frame = 0

Query: 894  MQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREIKTKAEQSETM 953
            MQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREIKTKAEQSETM
Sbjct: 1    MQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREIKTKAEQSETM 60

Query: 954  VQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQL 1013
            VQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQL
Sbjct: 61   VQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEAAAQLEAVNQL 120

Query: 1014 CSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLLQQLSDACFVV 1073
            CSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLLQQLSDACFVV
Sbjct: 121  CSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLLQQLSDACFVV 180

Query: 1074 DALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFP 1133
            DALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFP
Sbjct: 181  DALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIRTNEEIWKIFP 240

Query: 1134 PSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFEDELAEKFGGGTR 1193
            PSWHVPYRLCIQFCKKTRKQLEDI+DNLKEKPDVATLLLALQRTLEFEDELAEKFGGG R
Sbjct: 241  PSWHVPYRLCIQFCKKTRKQLEDILDNLKEKPDVATLLLALQRTLEFEDELAEKFGGGAR 300

Query: 1194 GKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKDGIKDMSVPGAGFNFRG 1253
            GKE+GN IEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEK+GIKDMSVPGAGFNFRG
Sbjct: 301  GKESGNGIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKNGIKDMSVPGAGFNFRG 360

Query: 1254 IVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQLFLIIKRSLK 1313
            IVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQLFLIIKRSLK
Sbjct: 361  IVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSMQLFLIIKRSLK 420

Query: 1314 RCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKV 1373
            RCSALTKNQTL NLFKVFQRVLKAYA KLFARLPKGGTGFVAAATGMDGQIKTSDKDEKV
Sbjct: 421  RCSALTKNQTLLNLFKVFQRVLKAYATKLFARLPKGGTGFVAAATGMDGQIKTSDKDEKV 480

Query: 1374 ICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKALVTLVHGLET 1433
            ICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKALVTLVHGLET
Sbjct: 481  ICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITKALVTLVHGLET 540

Query: 1434 KFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLYFQFFLDKLASS 1493
            KFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPV GRLLSPLYFQFFLDKLASS
Sbjct: 541  KFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVFGRLLSPLYFQFFLDKLASS 600

Query: 1494 LGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSSAASYSKFVSREMSK 1553
            LGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTS AASYSKFVSREMSK
Sbjct: 601  LGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSGAASYSKFVSREMSK 660

Query: 1554 AEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILDDFNKHGPGIT 1613
            AEALLKVILSP+DSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILDDFNKHGPGIT
Sbjct: 661  AEALLKVILSPVDSVADTYRALLPEGTPMEFQRILELKGFKKADQQSILDDFNKHGPGIT 720

Query: 1614 QPSV-SPSAPPVASSTPPAPTITSP-TVGLMASREDVLTRAAALGRGAATTGFKRFLALT 1673
            QPSV SPSAPPV SSTPPAPT+TSP TVGLMASREDVLTRAAALGRGAATTGFKRFLALT
Sbjct: 721  QPSVSSPSAPPVVSSTPPAPTVTSPSTVGLMASREDVLTRAAALGRGAATTGFKRFLALT 780

Query: 1674 EAAKDRKDGPFRKLFNP 1689
            EAAKDRKDGPFRKLFNP
Sbjct: 781  EAAKDRKDGPFRKLFNP 797

BLAST of Lag0037683 vs. TAIR 10
Match: AT1G50500.1 (Membrane trafficking VPS53 family protein )

HSP 1 Score: 1256.5 bits (3250), Expect = 0.0e+00
Identity = 665/812 (81.90%), Postives = 727/812 (89.53%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            EASL+GVEPLMQKI  EIRRVDA IL+AVRQQS+SGTKAKEDLA AT AVEEL  KI+EI
Sbjct: 17   EASLTGVEPLMQKIQGEIRRVDASILSAVRQQSNSGTKAKEDLADATRAVEELSHKIQEI 76

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            K+KAEQSE MVQEICRDIKKLDFAKK+ITTTITALHRLTMLVSAVEQLQVMASKRQYKEA
Sbjct: 77   KSKAEQSEAMVQEICRDIKKLDFAKKNITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 136

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 1063
            AAQLEAVNQLC+HFEAYRD PKITELREK  NIKQILKSHVFSDFSSLGTGKE EETNLL
Sbjct: 137  AAQLEAVNQLCNHFEAYRDVPKITELREKLNNIKQILKSHVFSDFSSLGTGKETEETNLL 196

Query: 1064 QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 1123
            Q+LSD+C VVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR
Sbjct: 197  QKLSDSCLVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 256

Query: 1124 TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFEDE 1183
            TNEEIWKIFP SWHVPYRLCIQFCK+TRKQ+E I+ N+KEKP VA LLLALQ T+EFE E
Sbjct: 257  TNEEIWKIFPASWHVPYRLCIQFCKQTRKQVESILVNMKEKPVVAILLLALQSTVEFEKE 316

Query: 1184 LAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPEND----EKDGI 1243
            L +KFGGG   K+  ++IEE G  + NSQN+S IRKKYEKK A  Q  E +    EK G 
Sbjct: 317  LEKKFGGGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKFAASQETEENGFQQEKTGN 376

Query: 1244 KDMSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLS 1303
            KD+SV GAGFNFRG++SSCFEPHLT YIELEEKTLM++LEK+VQEE+WD+++GSQ+NVLS
Sbjct: 377  KDLSVTGAGFNFRGMISSCFEPHLTPYIELEEKTLMDDLEKIVQEESWDVEDGSQNNVLS 436

Query: 1304 SSMQLFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATG 1363
            SS QLF  IK+SLKRC+ L+KNQTLFNLFKVFQRVLKAYA KLF +LPKGGTG VAAATG
Sbjct: 437  SSTQLFSNIKKSLKRCNTLSKNQTLFNLFKVFQRVLKAYATKLFFKLPKGGTGIVAAATG 496

Query: 1364 MDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAV 1423
            MDGQIK S++DE+VICYIVNSAEYCHKTSGELAE+V +IID    DGVDMSEVQDEFSAV
Sbjct: 497  MDGQIKVSERDERVICYIVNSAEYCHKTSGELAENVSEIIDPHYADGVDMSEVQDEFSAV 556

Query: 1424 ITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLS 1483
            ITKALVTLV GLETKFD+EMA MTRVPW TLESVGDQS YVNGIN +L+ SIPVLG+LL+
Sbjct: 557  ITKALVTLVLGLETKFDTEMAVMTRVPWSTLESVGDQSGYVNGINTVLSGSIPVLGKLLT 616

Query: 1484 PLYFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSS 1543
            P+YFQFFLDKLASSLGPRFYANIF+CKQ+SETGAQQMLLDTQAVK+ILL+IPSL RQTS+
Sbjct: 617  PVYFQFFLDKLASSLGPRFYANIFRCKQLSETGAQQMLLDTQAVKSILLEIPSLARQTST 676

Query: 1544 AASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQ 1603
            AASYSKFVSREMS+AEALLKVILSPIDSVADTYRAL PEGTPMEFQRILELKG KKADQQ
Sbjct: 677  AASYSKFVSREMSRAEALLKVILSPIDSVADTYRALFPEGTPMEFQRILELKGLKKADQQ 736

Query: 1604 SILDDFNKHGPGITQPSVSPSAPPVASSTPPAP--TITSP--TVGLMASREDVLTRAAAL 1663
            SILDDFNKHGPG TQ SV+ +A P    TPPAP   IT+P    G +A+ EDVLTRAAAL
Sbjct: 737  SILDDFNKHGPGFTQQSVA-AAMPQPMPTPPAPPLAITNPATAAGFIANSEDVLTRAAAL 796

Query: 1664 GRGAATTGFKRFLALTEAAKDRKDGPFRKLFN 1688
            GRGAA+TGFK+F+ALTEAAKDRKDGP R+LFN
Sbjct: 797  GRGAASTGFKKFIALTEAAKDRKDGPLRRLFN 827

BLAST of Lag0037683 vs. TAIR 10
Match: AT1G50500.2 (Membrane trafficking VPS53 family protein )

HSP 1 Score: 1256.5 bits (3250), Expect = 0.0e+00
Identity = 665/812 (81.90%), Postives = 727/812 (89.53%), Query Frame = 0

Query: 884  EASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIREI 943
            EASL+GVEPLMQKI  EIRRVDA IL+AVRQQS+SGTKAKEDLA AT AVEEL  KI+EI
Sbjct: 36   EASLTGVEPLMQKIQGEIRRVDASILSAVRQQSNSGTKAKEDLADATRAVEELSHKIQEI 95

Query: 944  KTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 1003
            K+KAEQSE MVQEICRDIKKLDFAKK+ITTTITALHRLTMLVSAVEQLQVMASKRQYKEA
Sbjct: 96   KSKAEQSEAMVQEICRDIKKLDFAKKNITTTITALHRLTMLVSAVEQLQVMASKRQYKEA 155

Query: 1004 AAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNLL 1063
            AAQLEAVNQLC+HFEAYRD PKITELREK  NIKQILKSHVFSDFSSLGTGKE EETNLL
Sbjct: 156  AAQLEAVNQLCNHFEAYRDVPKITELREKLNNIKQILKSHVFSDFSSLGTGKETEETNLL 215

Query: 1064 QQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 1123
            Q+LSD+C VVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR
Sbjct: 216  QKLSDSCLVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRIR 275

Query: 1124 TNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFEDE 1183
            TNEEIWKIFP SWHVPYRLCIQFCK+TRKQ+E I+ N+KEKP VA LLLALQ T+EFE E
Sbjct: 276  TNEEIWKIFPASWHVPYRLCIQFCKQTRKQVESILVNMKEKPVVAILLLALQSTVEFEKE 335

Query: 1184 LAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPEND----EKDGI 1243
            L +KFGGG   K+  ++IEE G  + NSQN+S IRKKYEKK A  Q  E +    EK G 
Sbjct: 336  LEKKFGGGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKFAASQETEENGFQQEKTGN 395

Query: 1244 KDMSVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLS 1303
            KD+SV GAGFNFRG++SSCFEPHLT YIELEEKTLM++LEK+VQEE+WD+++GSQ+NVLS
Sbjct: 396  KDLSVTGAGFNFRGMISSCFEPHLTPYIELEEKTLMDDLEKIVQEESWDVEDGSQNNVLS 455

Query: 1304 SSMQLFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATG 1363
            SS QLF  IK+SLKRC+ L+KNQTLFNLFKVFQRVLKAYA KLF +LPKGGTG VAAATG
Sbjct: 456  SSTQLFSNIKKSLKRCNTLSKNQTLFNLFKVFQRVLKAYATKLFFKLPKGGTGIVAAATG 515

Query: 1364 MDGQIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAV 1423
            MDGQIK S++DE+VICYIVNSAEYCHKTSGELAE+V +IID    DGVDMSEVQDEFSAV
Sbjct: 516  MDGQIKVSERDERVICYIVNSAEYCHKTSGELAENVSEIIDPHYADGVDMSEVQDEFSAV 575

Query: 1424 ITKALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLS 1483
            ITKALVTLV GLETKFD+EMA MTRVPW TLESVGDQS YVNGIN +L+ SIPVLG+LL+
Sbjct: 576  ITKALVTLVLGLETKFDTEMAVMTRVPWSTLESVGDQSGYVNGINTVLSGSIPVLGKLLT 635

Query: 1484 PLYFQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQTSS 1543
            P+YFQFFLDKLASSLGPRFYANIF+CKQ+SETGAQQMLLDTQAVK+ILL+IPSL RQTS+
Sbjct: 636  PVYFQFFLDKLASSLGPRFYANIFRCKQLSETGAQQMLLDTQAVKSILLEIPSLARQTST 695

Query: 1544 AASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFKKADQQ 1603
            AASYSKFVSREMS+AEALLKVILSPIDSVADTYRAL PEGTPMEFQRILELKG KKADQQ
Sbjct: 696  AASYSKFVSREMSRAEALLKVILSPIDSVADTYRALFPEGTPMEFQRILELKGLKKADQQ 755

Query: 1604 SILDDFNKHGPGITQPSVSPSAPPVASSTPPAP--TITSP--TVGLMASREDVLTRAAAL 1663
            SILDDFNKHGPG TQ SV+ +A P    TPPAP   IT+P    G +A+ EDVLTRAAAL
Sbjct: 756  SILDDFNKHGPGFTQQSVA-AAMPQPMPTPPAPPLAITNPATAAGFIANSEDVLTRAAAL 815

Query: 1664 GRGAATTGFKRFLALTEAAKDRKDGPFRKLFN 1688
            GRGAA+TGFK+F+ALTEAAKDRKDGP R+LFN
Sbjct: 816  GRGAASTGFKKFIALTEAAKDRKDGPLRRLFN 846

BLAST of Lag0037683 vs. TAIR 10
Match: AT1G50970.1 (Membrane trafficking VPS53 family protein )

HSP 1 Score: 530.4 bits (1365), Expect = 5.3e-150
Identity = 368/814 (45.21%), Postives = 422/814 (51.84%), Query Frame = 0

Query: 883  LEASLSGVEPLMQKIHNEIRRVDAGILAAVRQQSSSGTKAKEDLAAATSAVEELMSKIRE 942
            +EASLS VE  MQKI +EIRRVDA ILA V QQ +SGT+AKE+L  A  A EEL  KI+E
Sbjct: 16   VEASLSCVESFMQKICDEIRRVDATILAVVSQQGNSGTRAKENLNDAICAAEELSHKIQE 75

Query: 943  IKTKAEQSETMVQEICRDIKKLDFAKKHITTTITALHRLTMLVSAVEQLQVMASKRQYKE 1002
            IK+KAEQ+E MVQ+IC DIKKLDFAKK+ITT +TAL RLTMLVSAV+QLQVM SKRQYKE
Sbjct: 76   IKSKAEQTEAMVQDICSDIKKLDFAKKNITTAVTALSRLTMLVSAVQQLQVMTSKRQYKE 135

Query: 1003 AAAQLEAVNQLCSHFEAYRDNPKITELREKFKNIKQILKSHVFSDFSSLGTGKEKEETNL 1062
            AA QLEA+N+LC+HF+AY D PKI ELREK KNIKQILK HVFSDFSSLGTG E EE  L
Sbjct: 136  AATQLEAINELCNHFKAYMDLPKIMELREKLKNIKQILKFHVFSDFSSLGTGTETEELFL 195

Query: 1063 LQQLSDACFVVDALEPSVREELVNNFCSRELTSYEQIFEGAELAKLDKTERRYAWIKRRI 1122
            L++LSD+C VVDALEPSVREEL+NNFCSRELTSYEQI+ GAEL  LD+ E  Y  +   I
Sbjct: 196  LKKLSDSCLVVDALEPSVREELINNFCSRELTSYEQIYVGAELKTLDEIELIYNQLSCLI 255

Query: 1123 RTNEEIWKIFPPSWHVPYRLCIQFCKKTRKQLEDIMDNLKEKPDVATLLLALQRTLEFED 1182
            R N+  W IFP SWHVPYRLCIQ  +KTR Q+E I+ NLKEK DV  LLL L+RTLEFE 
Sbjct: 256  RKNQGKWTIFPASWHVPYRLCIQLSRKTRVQVESILVNLKEKSDVEKLLLELKRTLEFER 315

Query: 1183 ELAEKFGGGTRGKENGNEIEEFGREDSNSQNVSDIRKKYEKKLAVHQGPENDEKDGIKDM 1242
            EL  KFGG   G   G++I   G   +NSQ                              
Sbjct: 316  ELEMKFGG---GGSIGDDI--IGGGGNNSQK----------------------------- 375

Query: 1243 SVPGAGFNFRGIVSSCFEPHLTVYIELEEKTLMENLEKLVQEETWDIDEGSQSNVLSSSM 1302
                  FNFRG++SSCFEPHLT+YIE EE  LM+ LEK+VQEETWDI+E           
Sbjct: 376  ------FNFRGMISSCFEPHLTIYIEKEEMELMQLLEKVVQEETWDIEEELG-------- 435

Query: 1303 QLFLIIKRSLKRCSALTKNQTLFNLFKVFQRVLKAYANKLFARLPKGGTGFVAAATGMDG 1362
                                                                        
Sbjct: 436  ------------------------------------------------------------ 495

Query: 1363 QIKTSDKDEKVICYIVNSAEYCHKTSGELAESVQKIIDSQLVDGVDMSEVQDEFSAVITK 1422
                                 CH                                     
Sbjct: 496  ---------------------CH------------------------------------- 555

Query: 1423 ALVTLVHGLETKFDSEMAAMTRVPWGTLESVGDQSEYVNGINMILTTSIPVLGRLLSPLY 1482
                                                                        
Sbjct: 556  ------------------------------------------------------------ 569

Query: 1483 FQFFLDKLASSLGPRFYANIFKCKQISETGAQQMLLDTQAVKTILLDIPSLGRQ------ 1542
                                      SE     MLLD   +K ILL +PSL RQ      
Sbjct: 616  --------------------------SENSVYLMLLDAHDMKMILLKVPSLARQPEASAL 569

Query: 1543 --TSSAASYSKFVSREMSKAEALLKVILSPIDSVADTYRALLPEGTPMEFQRILELKGFK 1602
               ++ ASY K V+ +M +AEA+LKVI SPI +V DTYRAL PE TPMEFQRIL LKG  
Sbjct: 676  LVKTATASYVKLVNHQMKRAEAVLKVIASPIVTVIDTYRALFPEETPMEFQRILVLKGLT 569

Query: 1603 KADQQSILDDFNKHGPGITQPSVSPSAPPVASSTPPAPTITSPTVGLMASREDVLTRAAA 1662
            KA+QQSILDDFN H   ITQ SV+   P  A + P A T  +P V   A+ E+VLTRAA+
Sbjct: 736  KAEQQSILDDFNNHSSRITQLSVAAKTPE-AHALPLALTNVAPAVRFKANSEEVLTRAAS 569

Query: 1663 LGRGAATTGFKRFLALTEAAKDRKDGPFRKLFNP 1689
                AATT F +  ALT AAKDR   PFRKLFNP
Sbjct: 796  ----AATTSFMKLYALTGAAKDR---PFRKLFNP 569

BLAST of Lag0037683 vs. TAIR 10
Match: AT3G29750.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.1e-10
Identity = 40/102 (39.22%), Postives = 55/102 (53.92%), Query Frame = 0

Query: 53  KTMKVRGVIKGQAVVVLIDSGATHNFISTEVVQKLGLEISPSSSYGIVLGTGGLVQRAGI 112
           K M+  G I    VVV IDSGAT NFI  E+   L L  S ++   ++LG    +Q  G 
Sbjct: 122 KGMRFYGFILDHKVVVAIDSGATDNFILVELAFSLKLPTSITNQASVLLGQRQCIQSVGT 181

Query: 113 CQGLILTISDLTIIHDFLPLPLG--SADMILGITWLETLGKS 153
           C G+ L + ++ I  +FL L L     D+ILG  WL  LG++
Sbjct: 182 CLGIRLWVQEVEITENFLLLDLAKTDVDVILGYEWLSKLGET 223

BLAST of Lag0037683 vs. TAIR 10
Match: AT3G30770.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.4e-09
Identity = 41/116 (35.34%), Postives = 60/116 (51.72%), Query Frame = 0

Query: 29  DGALVENSEVAALSLHSMVGFDSPKTMKVRGVIKGQAVVVLIDSGATHNFISTEVVQKLG 88
           +G L +   +  +   S   F   K M+  G I    VVV+IDSGAT+NFIS E+   L 
Sbjct: 258 EGLLEDFKTIRQVKRQSTTEFTKGKDMRFYGFISCHKVVVVIDSGATNNFISDELALVLK 317

Query: 89  LEISPSSSYGIVLGTGGLVQRAGICQGLILTISDLTIIHDFLPLPL--GSADMILG 143
           L  S ++   ++LG    +Q  G C G+ L + ++ I  +FL L L     D+ILG
Sbjct: 318 LPTSTTNQASVLLGQRQCIQTIGTCFGINLLVQEVEINENFLLLDLTKTDVDVILG 373