Lag0037682 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0037682
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDEAD-box ATP-dependent RNA helicase
Locationchr2: 8303794 .. 8308108 (+)
RNA-Seq ExpressionLag0037682
SyntenyLag0037682
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGAATTCAATTCTTCTACGGAGATCCGCTGCCTTAGCGGCCTCCAGAGCTAGAGTTAGCTCCGCCTTGTTAGCCCCAATCGCGAACTTGTTAGCGTCTTCTGTGGACGTTAATGGCGGCGTTATACCTGCTGCTGAATTTGGACATTTCTCCAGTATTGGGGTGATGGAGAGGCCTTTGGGTTTTGGTTTTCAATCGAAGGGATTCCATGCGGCTTCTGGGCCGTTGAATTTCAAGGCGTCGTTGGTCTCCCAGGCGGAGTTTGCGGTGGAGGAGTATGATGACGCTAGCAGCAGTAGAAGCGCCGATGAAGGACTCGAGATTGGAAAGCTCGGGATCGCGCCGGAGATTGTTTCTGCTCTGGCGAAAAAGGGTATAACGAAGCTCTTCCCTATTCAGGTACTGTTTTGCTCATTTCTTTCATGCTTTACTAGTTTTTGCTGCTGGGGTTAGACCACATGGACAGAGTCGTGGAGAAATTTTTGAAAATTAGTTTCGATGATTTTGATCACTGCGGAGTTTGGACCCTTTGGACGTCGAATTACTGCGATGGAGTTACAATTTTATTTGTTATGAACGAATTTCATTTGTTTTGAGAGCTAGAGGCCATGTTCCTTACTTCGAGACCTTTTGGCTGTACGTGATACCTGTATCCCGGAATATGCATATCGTTCCCATTCCTCTGTCGAATAATGTATTTGACATGTTTTGGTTTGGAATTCTAATAGTTCCGTTTGGTTATGTTTCAAACTTGTGTTTCATTTACCATTGGAGTTTGAAAATGTGCTATTTTCTCAATCTTTTTCAGAGAGCCGTGCTAGAACCAGCAATGCAAGGCCGGGACATGATTGGTCGTGCTAGAACTGGAACAGGAAAAACTCTTGCTTTTGGAATTCCCATCTTGGACAAAATCATTCAGTTCAATGCGAAACATGGGTTTGTGTTTTAATCTCGTTTTTTTTTCTCTCCCTCCAACTAGCTCTAATTCTTTCTGCTGAATTATTCATCTGGGAAGGGTTTGGATGGTTATTCTTTAAATTTTAAAAATCACAATTTGTTAAAAATTATGCAGAAGAGGAAGGAACCCGTTGGCCCTGGTTCTAGCTCCAACCAGGGAACTTGCTCGGCAGGTTGAAAAAGAATTTCAAGAGGCTGCACCTAGCCTGGACACTATCTGTGTTTATGGGGGGCACCAATTTCACGCCAAATGAGGCAACTTGACTTTGGCGTTGACGTAGCTGTTGGCACACCTGGTCGCTTGATTGATCTTCTTAATAGGGGTTCGCTAAATTTATCAGAAGTTCAGTTTGTTGTACTTGATGAAGCAGACCAGATGCTTCAAGTAGGTTTTCAAGAGGACGTTGAGAAGATCTTGGAGAGGTTGCCTCAGAAACGCCAGAGTATGATGTTCTCTGCAACAATGCCATCTTGGATCAGAAAACTCACTCAAAATTACCTAACTAATCCATTAACTATTGATCTTGTAAGTATGTTGTCTATTTGATATATTTTCTACTTCTTTGAATTTAATTATATCACTGAAAAGCACAACCATCTAATCAGTAGACTCAGACATTGAAGGTGGGCTGTGCCCATTATTTTCTGAATAAGGAAAAATGCTCTTGCTAATCAAAATCAATATTCTTTTTTCTCATGAAGTTCGGTTGTAGCTTGTAGGATTAAAAGTTCAAAAAATTCTTAAAACACTTAAAAATTTTTGTGTTCTCTTGGAAGGTACTTATAAAAGTACATTTTCCATTTTTTTTTTTCTTTAGAGATAAAAAGCATTTTACTCAGAAGAACTAAGATCTTTCTGAACTCACTCATAGCTTAGAGCTGCTTGCTTGTTTTTTTTGTTTTTGTTTTTTGTTTTTTTGTTTTGTTTTGTTTTGTTTTGTGTGTGTGTGTGTGTGGGTGTGAACACACACACAGGAGCTAGACCTCAGGAAGTGGAAAAAGAAAACATTTGTTTGATTTTTTGTTTTTCTGTTAATATGTATATAGTTTAGAAGCTAGACCTCAGGAAAGTGGTAAAAGAAAACATTCTCTTTAAAAAGAACAATGTTCTTTCTTTCTGTTGTATGGGTTAGTGTCTGTGAAAGAAGTTCTTTATTGCATTTTGAATGTTATGATCTATCTTCGGAAGCATTTTTTTCATTTATTCATGGGGCCTGAGAGAGTAAATTTTTAAAAAAACTAAAGGAAATGAAAAAAAAAGAGGAAAATCTCATCACTATTTCATTGATACATGGGGATTTGACAAGGCTTATCAATTTTTCAGGTTGGTGATTCTGATCAGAAATTGGCAGATGGAATTAGCTTATTTTCAATTGCTGCAGATATGCATGGAAAAGCATCAATAATTGGACCTCTGATAACAGTATGGATCCATAACTTATGCGTTTCTTTTTTCCCTTTGATTTCTTCGAGGCAATATTTTACCTGGTTCTGCTATCATGTTGTTGACTGCTTGCTTTCATTATGTGCTGTTGCCAATGATTATCTGCTCATCAGGAACATGCGAAAGGAGGAAAGTGTATTGTCTTCACTCAAACAAAGCGTGATGCTGATAGATTAGCCTATGCAATGGCAAGGAACTTTAGATGTGAAGCTTTGCATGGAGATATTTCACAGAGTCAGAGAGAAAGAACTCTTTCTGGTTTTAGAGATGGACATTTCAACATTTTAGTTGCAACTGATGTTGCAGCAAGGGGTCTTGATATACCCAATGTGGATCTTGTAAGTGATAATCTTCCTCTATATTTCTTCCTCAGTTTTATCTTCCTTTATGGTACATATTTCTGGATGCATGGTGAATCATTAAATGCTTTCTTCTCGATAATTTACCTTCCTTCAACTTTGAAAATAAACTAGAGCACTGTAATAGACTAATAGTAGTATCAGACCCTATCATCTCTCTTCCTCTTGTGCTGTTGGGAGCCTGGGAGGAAGTGGATTGAAGGATGAGGAAGTATTTCGTTTGTATGTGGGTTAGTTATTAAGATTCTAAGGATTTTTTCTGACCTCGATTTTGTCTTAGGTAATACATTTTGAGCTTCCAAACAACACAGAGATATTTGTTCATAGATCAGGCCGTACAGGTCGTGCTGGTAAGAAAGGCAGTGCTATTCTTATTTACTCACAGGATCAGTCAAGGGCTGTAAAAATGATTGAGCGTGAAGTGGGCTGCAGATTTACTGAGGTTAGGATATCTCTTCAGCATCAAACATAAACCTTGGGATTTTTTAATATAAAACTGGAACTTCCATCAATGTTCTATATTTTTACACATGAGAAATATTAATCTCCCTTGAGAACATTTCTAGTTTGCTTGCAGTAAGTTTCTGGCTCCAGTAACAGCTTCTAAATAGATGCCTGGATGCCCTGGTTACTGGTTCGTGCATTGTTTAGAAGTTGAGGATTCATATTTCAAGCTTATCTCATATACAAAATAAACCAAGCCTATCACATTTTCACATGCTAGATTTTTATCCCTGAATTTTAGAATTAAAATTGAGATGGATTGTTCTCTAATATCACATGATAAGCAATCAACACCCGTAGACAACCATAACGAGTGTTTTCTGACGGGTGGATTGCTATGCACATTTGGGTTTTATGAAAATTTTAATCTACAATTCATGCGTATGAATATTGTGCAAATGCAGATATATATATATATATTTTTTTAAAAATGACTTTTGAAGATTTAGAGGCATTTGTTTTGATTATGAAATAACTGTCCCATAATCTGTATAGTTGAGTAAATTCAGGCATACCAATCACTATAGGGTGCTATATAATTAGATAATTGACTTCTCTAGCAGCTTCCCGTCTCAGGCATTTACAACAAATATTGCTCCACCTTGGAGTATATATTACTAGATGGAAAGTTGACACTGATTGCTTTGTTCTTGATTATCAGCTTCCAAGGATTGCTGTTGAGGATGGTGCTCGTGTGGATATGTTCAGTGACAGAGGTGGTGGTGGTCAATTTGGCTCTTTTGGAGGATTTAGGGATCGCCGAATGAATGATTCAGGGCGTTTTGGTGGCCAAAAAGGTGGTTCAAATTTTGGCCGTTCAGGAGGTTATGAAAATATCAAACGTTCCAGTGTCTGGGATTCTTTTGGGCAAATGGGTAGAAATAATGGACCTAGCTCTGGTGGTTTTGGTTCATTTGGTGGTGGTCGTAGCTCTGGCTCTGCATTTGGTGGAAATTTTGGTAGTTCAGGGTCTGGACGTTCTGGTGGTTTTGGCGATTTTGGTTCGGGTCGAAGTTCAGGATTTGGAGACAGAAATTCTAGAAACTAG

mRNA sequence

ATGATGAATTCAATTCTTCTACGGAGATCCGCTGCCTTAGCGGCCTCCAGAGCTAGAGTTAGCTCCGCCTTGTTAGCCCCAATCGCGAACTTGTTAGCGTCTTCTGTGGACGTTAATGGCGGCGTTATACCTGCTGCTGAATTTGGACATTTCTCCAGTATTGGGGTGATGGAGAGGCCTTTGGGTTTTGGTTTTCAATCGAAGGGATTCCATGCGGCTTCTGGGCCGTTGAATTTCAAGGCGTCGTTGGTCTCCCAGGCGGAGTTTGCGGTGGAGGAGTATGATGACGCTAGCAGCAGTAGAAGCGCCGATGAAGGACTCGAGATTGGAAAGCTCGGGATCGCGCCGGAGATTGTTTCTGCTCTGGCGAAAAAGGGTATAACGAAGCTCTTCCCTATTCAGAGAGCCGTGCTAGAACCAGCAATGCAAGGCCGGGACATGATTGGTCGTGCTAGAACTGGAACAGGAAAAACTCTTGCTTTTGGAATTCCCATCTTGGACAAAATCATTCAGTTCAATGCGAAACATGGAAGAGGAAGGAACCCGTTGGCCCTGGTTCTAGCTCCAACCAGGGAACTTGCTCGGCAGGTTGAAAAAGAATTTCAAGAGGCTGCACCTAGCCTGGACACTATCTCTGTTGGCACACCTGGTCGCTTGATTGATCTTCTTAATAGGGGTTCGCTAAATTTATCAGAAGTTCAGTTTGTTGTACTTGATGAAGCAGACCAGATGCTTCAAGTAGGTTTTCAAGAGGACGTTGAGAAGATCTTGGAGAGGTTGCCTCAGAAACGCCAGAGTATGATGTTCTCTGCAACAATGCCATCTTGGATCAGAAAACTCACTCAAAATTACCTAACTAATCCATTAACTATTGATCTTGTTGGTGATTCTGATCAGAAATTGGCAGATGGAATTAGCTTATTTTCAATTGCTGCAGATATGCATGGAAAAGCATCAATAATTGGACCTCTGATAACAGAACATGCGAAAGGAGGAAAGTGTATTGTCTTCACTCAAACAAAGCGTGATGCTGATAGATTAGCCTATGCAATGGCAAGGAACTTTAGATGTGAAGCTTTGCATGGAGATATTTCACAGAGTCAGAGAGAAAGAACTCTTTCTGGTTTTAGAGATGGACATTTCAACATTTTAGTTGCAACTGATGTTGCAGCAAGGGGTCTTGATATACCCAATGTGGATCTTGTAATACATTTTGAGCTTCCAAACAACACAGAGATATTTGTTCATAGATCAGGCCGTACAGGTCGTGCTGGTAAGAAAGGCAGTGCTATTCTTATTTACTCACAGGATCAGTCAAGGGCTGTAAAAATGATTGAGCGTGAAGTGGGCTGCAGATTTACTGAGCTTCCAAGGATTGCTGTTGAGGATGGTGCTCGTGTGGATATGTTCAGTGACAGAGGTGGTGGTGGTCAATTTGGCTCTTTTGGAGGATTTAGGGATCGCCGAATGAATGATTCAGGGCGTTTTGGTGGCCAAAAAGGTGGTTCAAATTTTGGCCGTTCAGGAGGTTATGAAAATATCAAACGTTCCAGTGTCTGGGATTCTTTTGGGCAAATGGGTAGAAATAATGGACCTAGCTCTGGTGGTTTTGGTTCATTTGGTGGTGGTCGTAGCTCTGGCTCTGCATTTGGTGGAAATTTTGGTAGTTCAGGGTCTGGACGTTCTGGTGGTTTTGGCGATTTTGGTTCGGGTCGAAGTTCAGGATTTGGAGACAGAAATTCTAGAAACTAG

Coding sequence (CDS)

ATGATGAATTCAATTCTTCTACGGAGATCCGCTGCCTTAGCGGCCTCCAGAGCTAGAGTTAGCTCCGCCTTGTTAGCCCCAATCGCGAACTTGTTAGCGTCTTCTGTGGACGTTAATGGCGGCGTTATACCTGCTGCTGAATTTGGACATTTCTCCAGTATTGGGGTGATGGAGAGGCCTTTGGGTTTTGGTTTTCAATCGAAGGGATTCCATGCGGCTTCTGGGCCGTTGAATTTCAAGGCGTCGTTGGTCTCCCAGGCGGAGTTTGCGGTGGAGGAGTATGATGACGCTAGCAGCAGTAGAAGCGCCGATGAAGGACTCGAGATTGGAAAGCTCGGGATCGCGCCGGAGATTGTTTCTGCTCTGGCGAAAAAGGGTATAACGAAGCTCTTCCCTATTCAGAGAGCCGTGCTAGAACCAGCAATGCAAGGCCGGGACATGATTGGTCGTGCTAGAACTGGAACAGGAAAAACTCTTGCTTTTGGAATTCCCATCTTGGACAAAATCATTCAGTTCAATGCGAAACATGGAAGAGGAAGGAACCCGTTGGCCCTGGTTCTAGCTCCAACCAGGGAACTTGCTCGGCAGGTTGAAAAAGAATTTCAAGAGGCTGCACCTAGCCTGGACACTATCTCTGTTGGCACACCTGGTCGCTTGATTGATCTTCTTAATAGGGGTTCGCTAAATTTATCAGAAGTTCAGTTTGTTGTACTTGATGAAGCAGACCAGATGCTTCAAGTAGGTTTTCAAGAGGACGTTGAGAAGATCTTGGAGAGGTTGCCTCAGAAACGCCAGAGTATGATGTTCTCTGCAACAATGCCATCTTGGATCAGAAAACTCACTCAAAATTACCTAACTAATCCATTAACTATTGATCTTGTTGGTGATTCTGATCAGAAATTGGCAGATGGAATTAGCTTATTTTCAATTGCTGCAGATATGCATGGAAAAGCATCAATAATTGGACCTCTGATAACAGAACATGCGAAAGGAGGAAAGTGTATTGTCTTCACTCAAACAAAGCGTGATGCTGATAGATTAGCCTATGCAATGGCAAGGAACTTTAGATGTGAAGCTTTGCATGGAGATATTTCACAGAGTCAGAGAGAAAGAACTCTTTCTGGTTTTAGAGATGGACATTTCAACATTTTAGTTGCAACTGATGTTGCAGCAAGGGGTCTTGATATACCCAATGTGGATCTTGTAATACATTTTGAGCTTCCAAACAACACAGAGATATTTGTTCATAGATCAGGCCGTACAGGTCGTGCTGGTAAGAAAGGCAGTGCTATTCTTATTTACTCACAGGATCAGTCAAGGGCTGTAAAAATGATTGAGCGTGAAGTGGGCTGCAGATTTACTGAGCTTCCAAGGATTGCTGTTGAGGATGGTGCTCGTGTGGATATGTTCAGTGACAGAGGTGGTGGTGGTCAATTTGGCTCTTTTGGAGGATTTAGGGATCGCCGAATGAATGATTCAGGGCGTTTTGGTGGCCAAAAAGGTGGTTCAAATTTTGGCCGTTCAGGAGGTTATGAAAATATCAAACGTTCCAGTGTCTGGGATTCTTTTGGGCAAATGGGTAGAAATAATGGACCTAGCTCTGGTGGTTTTGGTTCATTTGGTGGTGGTCGTAGCTCTGGCTCTGCATTTGGTGGAAATTTTGGTAGTTCAGGGTCTGGACGTTCTGGTGGTTTTGGCGATTTTGGTTCGGGTCGAAGTTCAGGATTTGGAGACAGAAATTCTAGAAACTAG

Protein sequence

MMNSILLRRSAALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIGVMERPLGFGFQSKGFHAASGPLNFKASLVSQAEFAVEEYDDASSSRSADEGLEIGKLGIAPEIVSALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGRNPLALVLAPTRELARQVEKEFQEAAPSLDTISVGTPGRLIDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRKLTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQTKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNVDLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRIAVEDGARVDMFSDRGGGGQFGSFGGFRDRRMNDSGRFGGQKGGSNFGRSGGYENIKRSSVWDSFGQMGRNNGPSSGGFGSFGGGRSSGSAFGGNFGSSGSGRSGGFGDFGSGRSSGFGDRNSRN
Homology
BLAST of Lag0037682 vs. NCBI nr
Match: XP_022981751.1 (DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 948.3 bits (2450), Expect = 3.0e-272
Identity = 529/627 (84.37%), Postives = 550/627 (87.72%), Query Frame = 0

Query: 1   MMNSILLRRSAALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIGVMERP 60
           MM+S+LLRRSAALAASR RVSS  LAPIAN L SSVDVNGGV+PAAEFG FS+ GV ER 
Sbjct: 1   MMSSVLLRRSAALAASRGRVSSTFLAPIANFLTSSVDVNGGVVPAAEFGRFSTTGVTERA 60

Query: 61  LGFGFQSKGFHAASGPLNFKASLVSQAEFAVEEYDDASSSRSADEGLEIGKLGIAPEIVS 120
           LGFGFQS GFHAASGPL+FK+SLVS+AEFAVEEYDDASSS+S DEGLEIG LGIAPEIVS
Sbjct: 61  LGFGFQSMGFHAASGPLSFKSSLVSRAEFAVEEYDDASSSKSGDEGLEIGSLGIAPEIVS 120

Query: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180
           ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR
Sbjct: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180

Query: 181 NPLALVLAPTRELARQVEKEFQEAAPSLDT---------------------ISVGTPGRL 240
            PLALVLAPTRELARQVEKEFQEAAP+LDT                     I+VGTPGRL
Sbjct: 181 YPLALVLAPTRELARQVEKEFQEAAPNLDTICVYGGAPISRQMRQLDYGVDIAVGTPGRL 240

Query: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRK 300
           IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQ+MMFSATMPSWIRK
Sbjct: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQTMMFSATMPSWIRK 300

Query: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQ 360
           LTQNYLTNPLTIDLVGDSDQKLADGISLFSI +DMHGKASIIGPLITEHAKGGKCIVFTQ
Sbjct: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIVSDMHGKASIIGPLITEHAKGGKCIVFTQ 360

Query: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNV 420
           TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFN+LVATDVAARGLDIPNV
Sbjct: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNVLVATDVAARGLDIPNV 420

Query: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRI 480
           DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAV+MIEREVGCRF ELPRI
Sbjct: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVRMIEREVGCRFNELPRI 480

Query: 481 AVEDGARVDMFSDRGGGGQFGSF-GGFRDRRMNDSGRFGGQKGGSNFGRSGGYEN--IKR 540
           +VE GA VDMFS   GGGQF SF GGFRDRRMNDSGRFGGQ+G S FGRS GYE+   +R
Sbjct: 481 SVESGAHVDMFS---GGGQFSSFGGGFRDRRMNDSGRFGGQRGPS-FGRSAGYESSGFRR 540

Query: 541 SSVWDSFGQMGRNNGPSSGGFGSFGGGRSSGSAFG-------GNFGSSGSGRSGGFGDF- 584
           SS  DSFGQ GRN+ PSSGGF SF G RSSGSAFG       G+FGS GSGRSGGFGDF 
Sbjct: 541 SSGRDSFGQGGRNSEPSSGGFSSF-GSRSSGSAFGGSSSKNAGSFGSFGSGRSGGFGDFG 600

BLAST of Lag0037682 vs. NCBI nr
Match: XP_023524742.1 (DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 941.8 bits (2433), Expect = 2.8e-270
Identity = 525/628 (83.60%), Postives = 548/628 (87.26%), Query Frame = 0

Query: 1   MMNSILLRRSAALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIGVMERP 60
           MM+S+LLRRSAALAASR RVSS  LAPIAN L SSVDVNGGV+PAAEFG FS+ GV ER 
Sbjct: 1   MMSSVLLRRSAALAASRGRVSSTFLAPIANFLTSSVDVNGGVVPAAEFGRFSTTGVTERA 60

Query: 61  LGFGFQSKGFHAASGPLNFKASLVSQAEFAVEEYDDASSSRSADEGLEIGKLGIAPEIVS 120
           LGFGFQS GFH+ASGPL+FK+SLVS+AEFAVE+YDDASSS+S DEGLEIG LGIAPEIVS
Sbjct: 61  LGFGFQSMGFHSASGPLSFKSSLVSRAEFAVEDYDDASSSKSGDEGLEIGSLGIAPEIVS 120

Query: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180
           ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR
Sbjct: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180

Query: 181 NPLALVLAPTRELARQVEKEFQEAAPSLDT---------------------ISVGTPGRL 240
            PLALVLAPTRELARQVEKEFQEAAP+LDT                     I+VGTPGRL
Sbjct: 181 YPLALVLAPTRELARQVEKEFQEAAPNLDTICVYGGAPISGQMRQLDYGVDIAVGTPGRL 240

Query: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRK 300
           IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQ+MMFSATMPSWIRK
Sbjct: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQTMMFSATMPSWIRK 300

Query: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQ 360
           LTQNYLTNPLTIDLVGDSDQKLADGISLFSI +DM GKASIIGPLITEHAKGGKCIVFTQ
Sbjct: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIVSDMRGKASIIGPLITEHAKGGKCIVFTQ 360

Query: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNV 420
           TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFN+LVATDVAARGLDIPNV
Sbjct: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNVLVATDVAARGLDIPNV 420

Query: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRI 480
           DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAV+MIEREVGCRF ELP+I
Sbjct: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVRMIEREVGCRFNELPKI 480

Query: 481 AVEDGARVDMFSDRGGGGQFGSF--GGFRDRRMNDSGRFGGQKGGSNFGRSGGYEN--IK 540
           +VE GA VDMFS   GGGQF SF  GGFRDRRMNDSGRFGGQ+G S FGRS GYEN   +
Sbjct: 481 SVESGAHVDMFS---GGGQFSSFGGGGFRDRRMNDSGRFGGQRGPS-FGRSAGYENSGFR 540

Query: 541 RSSVWDSFGQMGRNNGPSSGGFGSFGGGRSSGSAFG-------GNFGSSGSGRSGGFGDF 584
           RS   DSFGQ GRN+ PSSGGF SF G RSSGSAFG       G+FGS GSGRSGGFGDF
Sbjct: 541 RSGGRDSFGQGGRNSEPSSGGFSSF-GSRSSGSAFGGSSSNNAGSFGSFGSGRSGGFGDF 600

BLAST of Lag0037682 vs. NCBI nr
Match: KAG7037512.1 (DEAD-box ATP-dependent RNA helicase 53 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 941.4 bits (2432), Expect = 3.7e-270
Identity = 525/628 (83.60%), Postives = 547/628 (87.10%), Query Frame = 0

Query: 1   MMNSILLRRSAALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIGVMERP 60
           MM+S+LLRRSAALAASR RVSS  LAPIAN L SSVDVNGGV+PAAEFG FS+ GV ER 
Sbjct: 1   MMSSVLLRRSAALAASRGRVSSTFLAPIANFLTSSVDVNGGVVPAAEFGRFSTTGVTERA 60

Query: 61  LGFGFQSKGFHAASGPLNFKASLVSQAEFAVEEYDDASSSRSADEGLEIGKLGIAPEIVS 120
           LGFGFQS GFH+ASGPL FK+SLVS+AEFAVEEYDDASSS+S D+GLEIG LGIAPEIVS
Sbjct: 61  LGFGFQSMGFHSASGPLGFKSSLVSRAEFAVEEYDDASSSKSGDDGLEIGSLGIAPEIVS 120

Query: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180
           ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR
Sbjct: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180

Query: 181 NPLALVLAPTRELARQVEKEFQEAAPSLDT---------------------ISVGTPGRL 240
            PLALVLAPTRELARQVEKEFQEAAP+LDT                     I+VGTPGRL
Sbjct: 181 YPLALVLAPTRELARQVEKEFQEAAPNLDTICVYGGAPISRQMRQLDYGVDIAVGTPGRL 240

Query: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRK 300
           IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQ+MMFSATMPSWIRK
Sbjct: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQTMMFSATMPSWIRK 300

Query: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQ 360
           LTQNYLTNPLTIDLVGDSDQKLADGISLFSI +DM GKASIIGPLITEHAKGGKCIVFTQ
Sbjct: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIVSDMRGKASIIGPLITEHAKGGKCIVFTQ 360

Query: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNV 420
           TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFN+LVATDVAARGLDIPNV
Sbjct: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNVLVATDVAARGLDIPNV 420

Query: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRI 480
           DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAV+MIEREVGCRF ELP+I
Sbjct: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVRMIEREVGCRFNELPKI 480

Query: 481 AVEDGARVDMFSDRGGGGQFGSF--GGFRDRRMNDSGRFGGQKGGSNFGRSGGYEN--IK 540
           +VE GA VDMFS   GGGQF SF  GGFRDRRMNDSGRFGGQ+G S FGRS GYEN   +
Sbjct: 481 SVESGAHVDMFS---GGGQFSSFGGGGFRDRRMNDSGRFGGQRGPS-FGRSAGYENSGFR 540

Query: 541 RSSVWDSFGQMGRNNGPSSGGFGSFGGGRSSGSAFG-------GNFGSSGSGRSGGFGDF 584
           RS   DSFGQ GRN+ PSSGGF SF G RSSGSAFG       G+FGS GSGRSGGFGDF
Sbjct: 541 RSGGRDSFGQGGRNSEPSSGGFSSF-GSRSSGSAFGGSSSNNAGSFGSFGSGRSGGFGDF 600

BLAST of Lag0037682 vs. NCBI nr
Match: XP_022981753.1 (DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 941.4 bits (2432), Expect = 3.7e-270
Identity = 524/611 (85.76%), Postives = 545/611 (89.20%), Query Frame = 0

Query: 1   MMNSILLRRSAALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIGVMERP 60
           MM+S+LLRRSAALAASR RVSS  LAPIAN L SSVDVNGGV+PAAEFG FS+ GV ER 
Sbjct: 1   MMSSVLLRRSAALAASRGRVSSTFLAPIANFLTSSVDVNGGVVPAAEFGRFSTTGVTERA 60

Query: 61  LGFGFQSKGFHAASGPLNFKASLVSQAEFAVEEYDDASSSRSADEGLEIGKLGIAPEIVS 120
           LGFGFQS GFHAASGPL+FK+SLVS+AEFAVEEYDDASSS+S DEGLEIG LGIAPEIVS
Sbjct: 61  LGFGFQSMGFHAASGPLSFKSSLVSRAEFAVEEYDDASSSKSGDEGLEIGSLGIAPEIVS 120

Query: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180
           ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR
Sbjct: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180

Query: 181 NPLALVLAPTRELARQVEKEFQEAAPSLDT---------------------ISVGTPGRL 240
            PLALVLAPTRELARQVEKEFQEAAP+LDT                     I+VGTPGRL
Sbjct: 181 YPLALVLAPTRELARQVEKEFQEAAPNLDTICVYGGAPISRQMRQLDYGVDIAVGTPGRL 240

Query: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRK 300
           IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQ+MMFSATMPSWIRK
Sbjct: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQTMMFSATMPSWIRK 300

Query: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQ 360
           LTQNYLTNPLTIDLVGDSDQKLADGISLFSI +DMHGKASIIGPLITEHAKGGKCIVFTQ
Sbjct: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIVSDMHGKASIIGPLITEHAKGGKCIVFTQ 360

Query: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNV 420
           TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFN+LVATDVAARGLDIPNV
Sbjct: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNVLVATDVAARGLDIPNV 420

Query: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRI 480
           DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAV+MIEREVGCRF ELPRI
Sbjct: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVRMIEREVGCRFNELPRI 480

Query: 481 AVEDGARVDMFSDRGGGGQFGSF-GGFRDRRMNDSGRFGGQKGGSNFGRSGGYEN--IKR 540
           +VE GA VDMFS   GGGQF SF GGFRDRRMNDSGRFGGQ+G S FGRS GYE+   +R
Sbjct: 481 SVESGAHVDMFS---GGGQFSSFGGGFRDRRMNDSGRFGGQRGPS-FGRSAGYESSGFRR 540

Query: 541 SSVWDSFGQMGRNNGPSSGG---FGSFGGGRSSGSAFGGNFGSSGSGRSGGFGDF-GSGR 584
           SS  DSFGQ GRN+ PSSGG   FGSFG GRS G    G+FGS  S RSGGFGDF GSGR
Sbjct: 541 SSGRDSFGQGGRNSEPSSGGAGSFGSFGSGRSGGF---GDFGS--SSRSGGFGDFGGSGR 600

BLAST of Lag0037682 vs. NCBI nr
Match: XP_022940069.1 (DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 932.9 bits (2410), Expect = 1.3e-267
Identity = 519/612 (84.80%), Postives = 541/612 (88.40%), Query Frame = 0

Query: 1   MMNSILLRRSAALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIGVMERP 60
           MM+S+LLRRSAALAASR RVSS  LAPIAN L SSVDVNGG +PAAEFG FS+ GV ER 
Sbjct: 1   MMSSVLLRRSAALAASRGRVSSTFLAPIANFLTSSVDVNGGAVPAAEFGRFSTTGVTERA 60

Query: 61  LGFGFQSKGFHAASGPLNFKASLVSQAEFAVEEYDDASSSRSADEGLEIGKLGIAPEIVS 120
           LGFGFQS GFH+ASGPL FK+SLVS+AEFAVEEYDDASSS+S D+GLEIG LGIAPEIVS
Sbjct: 61  LGFGFQSMGFHSASGPLGFKSSLVSRAEFAVEEYDDASSSKSGDDGLEIGSLGIAPEIVS 120

Query: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180
           ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR
Sbjct: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180

Query: 181 NPLALVLAPTRELARQVEKEFQEAAPSLDT---------------------ISVGTPGRL 240
            PLALVLAPTRELARQVEKEFQEAAP+LDT                     I+VGTPGRL
Sbjct: 181 YPLALVLAPTRELARQVEKEFQEAAPNLDTICVYGGAPISRQMRQLDYGVDIAVGTPGRL 240

Query: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRK 300
           IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQ+MMFSATMPSWIRK
Sbjct: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQTMMFSATMPSWIRK 300

Query: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQ 360
           LTQNYLTNPLTIDLVGDSDQKLADGISLFSI +DM GKASIIGPLITEHAKGGKCIVFTQ
Sbjct: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIVSDMRGKASIIGPLITEHAKGGKCIVFTQ 360

Query: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNV 420
           TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFN+LVATDVAARGLDIPNV
Sbjct: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNVLVATDVAARGLDIPNV 420

Query: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRI 480
           DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAV+MIEREVGCRF ELP+I
Sbjct: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVRMIEREVGCRFNELPKI 480

Query: 481 AVEDGARVDMFSDRGGGGQFGSF--GGFRDRRMNDSGRFGGQKGGSNFGRSGGYEN--IK 540
           +VE GA VDMFS   GGGQF SF  GGFRDRRMNDSGRFGGQ+G S FGRS GYEN   +
Sbjct: 481 SVESGAHVDMFS---GGGQFSSFGGGGFRDRRMNDSGRFGGQRGPS-FGRSAGYENSGFR 540

Query: 541 RSSVWDSFGQMGRNNGPSSGG---FGSFGGGRSSGSAFGGNFGSSGSGRSGGFGDF-GSG 584
           RS   DSFGQ GRN+ PSSGG   FGSFG GRS G    G+FGS  S RSGGFGDF GSG
Sbjct: 541 RSGGRDSFGQGGRNSEPSSGGAGSFGSFGSGRSGGF---GDFGS--SSRSGGFGDFGGSG 600

BLAST of Lag0037682 vs. ExPASy Swiss-Prot
Match: Q9LUW5 (DEAD-box ATP-dependent RNA helicase 53, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RH53 PE=1 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 7.6e-194
Identity = 397/607 (65.40%), Postives = 450/607 (74.14%), Query Frame = 0

Query: 22  SALLAPIANLLASSVDVN----GGVIPAA-EFGHFSSIGVMERPLG--FGFQSKGFHAAS 81
           S L A   NL AS   +N      + PAA      + IG  +   G  FG ++KG H  S
Sbjct: 9   SLLDASKRNLSASLTSINTVLFHNLAPAATRVSDLALIGSSDVKAGFPFGVEAKGIHFQS 68

Query: 82  GPLNFKASLVSQAEFAVEEYDD---ASSSRSADEGLEIGKLGIAPEIVSALAKKGITKLF 141
           GPL+F+AS+VSQA FA+ E  +     S     +GL I +LGI+PEIV AL+ KGI KLF
Sbjct: 69  GPLDFRASMVSQAGFAISESSERRVGDSESVGGDGLAISELGISPEIVKALSSKGIEKLF 128

Query: 142 PIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGRNPLALVLAPTR 201
           PIQ+AVLEPAM+GRDMIGRARTGTGKTLAFGIPI+DKII++NAKHGRGRNPL LVLAPTR
Sbjct: 129 PIQKAVLEPAMEGRDMIGRARTGTGKTLAFGIPIIDKIIKYNAKHGRGRNPLCLVLAPTR 188

Query: 202 ELARQVEKEFQEAAPSLDTI---------------------SVGTPGRLIDLLNRGSLNL 261
           ELARQVEKEF+E+APSLDTI                     +VGTPGR+IDL+ RG+LNL
Sbjct: 189 ELARQVEKEFRESAPSLDTICLYGGTPIGQQMRQLDYGVDVAVGTPGRVIDLMKRGALNL 248

Query: 262 SEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRKLTQNYLTNPLT 321
           SEVQFVVLDEADQMLQVGF EDVE ILE+LP+KRQSMMFSATMPSWIR LT+ YL NPLT
Sbjct: 249 SEVQFVVLDEADQMLQVGFAEDVEIILEKLPEKRQSMMFSATMPSWIRSLTKKYLNNPLT 308

Query: 322 IDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQTKRDADRLAYA 381
           +DLVGDSDQKLADGI+ +SI AD +G+ASIIGPL+TEHAKGGKCIVFTQTKRDADRL+YA
Sbjct: 309 VDLVGDSDQKLADGITTYSIIADSYGRASIIGPLVTEHAKGGKCIVFTQTKRDADRLSYA 368

Query: 382 MARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNVDLVIHFELPNN 441
           +AR+F+CEALHGDISQSQRERTL+GFRDGHFNILVATDVAARGLD+PNVDL+IH+ELPNN
Sbjct: 369 LARSFKCEALHGDISQSQRERTLAGFRDGHFNILVATDVAARGLDVPNVDLIIHYELPNN 428

Query: 442 TEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRIAVEDGARVDMF 501
           TE FVHR+GRTGRAGKKGSAILIYSQDQSRAVK+IEREVG RFTELP IAVE G+   MF
Sbjct: 429 TETFVHRTGRTGRAGKKGSAILIYSQDQSRAVKIIEREVGSRFTELPSIAVERGS-ASMF 488

Query: 502 SDRG--GGGQFGSFGGFRDRRMNDSGR-----FGGQKGGSNFGRSGG----YENIKRSSV 561
              G   GG FG  GG RDR  +  GR     +GG  GG   GRSGG    Y      S 
Sbjct: 489 EGIGSRSGGSFG--GGMRDRGSSFGGRSGGGGYGGSSGGYGGGRSGGSSNRYSGDSDRSG 548

Query: 562 WDSFGQ-----MGRNNGPSSGGFGSFGGGRSSGSAFGGNFGSSGSGRSGGFGDFGSGRSS 582
           + SFG       G +    SGG  SFGGGRS GS+         + RS GFGDFGS RSS
Sbjct: 549 FGSFGMRSPEGYGSDRSSQSGGRSSFGGGRSGGSS---------NNRSSGFGDFGSDRSS 603

BLAST of Lag0037682 vs. ExPASy Swiss-Prot
Match: Q9LUW6 (DEAD-box ATP-dependent RNA helicase 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RH9 PE=1 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 4.0e-179
Identity = 381/623 (61.16%), Postives = 446/623 (71.59%), Query Frame = 0

Query: 2   MNSILLRRS------AALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIG 61
           M S +LRRS        LAAS   +++AL   +A   A+  D+  G          SS  
Sbjct: 1   MISTVLRRSILGTSRRTLAASVTSINAALFHNLAPAAATVSDLANGATNVKSLPSNSS-- 60

Query: 62  VMERPLGFGFQSKGFHAASGPLNFKASLVSQAEFAVEEY------------DDASSSRSA 121
                  FG + + FH  S P  F++S+VS A FA +EY             ++  S   
Sbjct: 61  ------PFGVKVRDFHVKSVPSEFRSSIVSSAGFAAQEYAPSYENDGGIGDSESVGSSGG 120

Query: 122 DEGLEIGKLGIAPEIVSALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGI 181
            +GL I  LGI+PEIV AL  +GI KLFPIQ+AVLEPAM+GRDMIGRARTGTGKTLAFGI
Sbjct: 121 GDGLAIADLGISPEIVKALKGRGIEKLFPIQKAVLEPAMEGRDMIGRARTGTGKTLAFGI 180

Query: 182 PILDKIIQFNAKHGRGRNPLALVLAPTRELARQVEKEFQEAAPSLDTI------------ 241
           PI+DKII+FNAKHGRG+NP  LVLAPTRELARQVEKEF+E+APSLDTI            
Sbjct: 181 PIIDKIIKFNAKHGRGKNPQCLVLAPTRELARQVEKEFRESAPSLDTICLYGGTPIGQQM 240

Query: 242 ---------SVGTPGRLIDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQ 301
                    +VGTPGR+IDL+ RG+LNLSEVQFVVLDEADQMLQVGF EDVE IL++LP 
Sbjct: 241 RELNYGIDVAVGTPGRIIDLMKRGALNLSEVQFVVLDEADQMLQVGFAEDVEIILQKLPA 300

Query: 302 KRQSMMFSATMPSWIRKLTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIG 361
           KRQSMMFSATMPSWIR LT+ YL NPLTIDLVGDSDQKLADGI+++SIAAD +G+ASIIG
Sbjct: 301 KRQSMMFSATMPSWIRSLTKKYLNNPLTIDLVGDSDQKLADGITMYSIAADSYGRASIIG 360

Query: 362 PLITEHAKGGKCIVFTQTKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFN 421
           PL+ EH KGGKCIVFTQTKRDADRLA+ +A++++CEALHGDISQ+QRERTL+GFRDG+F+
Sbjct: 361 PLVKEHGKGGKCIVFTQTKRDADRLAFGLAKSYKCEALHGDISQAQRERTLAGFRDGNFS 420

Query: 422 ILVATDVAARGLDIPNVDLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAV 481
           ILVATDVAARGLD+PNVDLVIH+ELPNNTE FVHR+GRTGRAGKKGSAILI+ QDQ+RAV
Sbjct: 421 ILVATDVAARGLDVPNVDLVIHYELPNNTETFVHRTGRTGRAGKKGSAILIHGQDQTRAV 480

Query: 482 KMIEREVGCRFTELPRIAVEDGARVDMFSDRGGGGQFGSFGGFRDRRMNDSGRFGGQKGG 541
           KMIE+EVG RF ELP IAVE G+   MF +  G    GSFGG R      SG  G    G
Sbjct: 481 KMIEKEVGSRFNELPSIAVERGS-ASMF-EGVGARSGGSFGGGR------SGGGGYGSYG 540

Query: 542 SNFGRSGGYENIKRSSVWDSFGQMGRNNGPSSGGFGSFGGGRSSGSAFGGNFGSSGSGRS 584
           S+ GRSGG           S+G  G ++G S GG GS+GG   S S + G     GS RS
Sbjct: 541 SSSGRSGG----------GSYGGYGGSSGRSGGGGGSYGGSGGSSSRYSG-----GSDRS 592

BLAST of Lag0037682 vs. ExPASy Swiss-Prot
Match: Q0ILZ4 (DEAD-box ATP-dependent RNA helicase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0611200 PE=2 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 7.6e-162
Identity = 354/625 (56.64%), Postives = 430/625 (68.80%), Query Frame = 0

Query: 6   LLRRSAALAASRARVSSALLAPIANLLASSVDV---NGGVIPAAEFGHFSSIGVMERPLG 65
           +LRR+A L   R R  SAL A +     ++ +V       IPAA F H S       P  
Sbjct: 4   ILRRAAPL---RRRAVSALAAAVLRREEAAAEVVVSRRATIPAAWF-HSS-------PAW 63

Query: 66  FGFQSKGFHAASGPLNFKASLVSQAEFAVEEYDDAS----SSRSADEGLEIGKLGIAPEI 125
            GF+  G   A+    + A    +  F  E+   A     ++  A+EGLE+ KLGI+P+I
Sbjct: 64  LGFRETGAAGAAARPQYAA---DEGLFYEEDKRGAKAGGVAAGGAEEGLEVAKLGISPKI 123

Query: 126 VSALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGR 185
           VS LA +GITKLFPIQRAVLEPAMQG+DM+GRA+TGTGKTLAFGIPILD II+ N K+  
Sbjct: 124 VSQLASRGITKLFPIQRAVLEPAMQGKDMVGRAKTGTGKTLAFGIPILDAIIRHNEKNSP 183

Query: 186 GRNPLALVLAPTRELARQVEKEFQEAAPSLDTISV---------------------GTPG 245
           G+ PLA+VLAPTRELA+QVE+EF +++ +++TI V                     GTPG
Sbjct: 184 GKFPLAIVLAPTRELAKQVEREFSDSS-NVETICVYGGTPISQQIRQLNYGVDVVIGTPG 243

Query: 246 RLIDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWI 305
           R+IDLL RG+LNLSEV+FVVLDEADQML VGF EDVE IL+R+P KRQ++MFSATMP+WI
Sbjct: 244 RVIDLLKRGALNLSEVRFVVLDEADQMLSVGFDEDVETILDRVPPKRQTLMFSATMPTWI 303

Query: 306 RKLTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVF 365
           ++LTQ YL NP+TIDLVG+ DQKLA+GISL+SIA++ H K +++G LI EHAKGGKCIVF
Sbjct: 304 QRLTQKYLKNPVTIDLVGEDDQKLAEGISLYSIASEGHAKPAVLGELIKEHAKGGKCIVF 363

Query: 366 TQTKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIP 425
           TQTKRDADRL+Y M R+F+C+ALHGDI+Q+QRERTL GFR+GHFNIL+ATDVAARGLDIP
Sbjct: 364 TQTKRDADRLSYTMGRSFQCQALHGDITQAQRERTLKGFREGHFNILIATDVAARGLDIP 423

Query: 426 NVDLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELP 485
           NVDLVIHFELPN++E+FVHRSGRTGRAGKKG AI+++S  QSRA++M+E +VGC+FTELP
Sbjct: 424 NVDLVIHFELPNSSELFVHRSGRTGRAGKKGKAIVMHSYQQSRAIRMVENDVGCKFTELP 483

Query: 486 RIAVEDGARVDMFSDRGGGGQFGSFGG---FRDRRMNDSGRFGGQKGGSNFGRSGGYENI 545
           +I VE    +    D  GGG FG  GG    R      S   GG  G S FGRSGG    
Sbjct: 484 KINVEGSDLMSGGFDSFGGGGFGREGGGSYGRRGSFGSSSSRGGGFGDSGFGRSGG--GF 543

Query: 546 KRS-------SVWDSFGQ--MGRNNGPSSGGFGSFGGGRSSGSAF--------GGNFGSS 582
            RS       S    FG    GR+ G   GGFG  G GRS G  +        GG  G S
Sbjct: 544 GRSGGGGFGRSSGGGFGDSGFGRSGG---GGFGDSGFGRSGGGGYGDSGFGSSGGGSGRS 603

BLAST of Lag0037682 vs. ExPASy Swiss-Prot
Match: Q0D8N0 (DEAD-box ATP-dependent RNA helicase 53 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0143700 PE=2 SV=2)

HSP 1 Score: 564.7 bits (1454), Expect = 1.2e-159
Identity = 350/632 (55.38%), Postives = 431/632 (68.20%), Query Frame = 0

Query: 1   MMNSILLRRSAALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIGVMERP 60
           +++  L   S++ AA R R   +LLA + +  AS +D   G  PAA           E P
Sbjct: 4   LLSRALCAASSSPAAPRGR---SLLAALLSPSASPLDPCRG--PAAP----------EPP 63

Query: 61  LGFGFQSKGFHAASGPLNFKASLVSQAEFAVEEYDDASSSRSADEGLEIGKLGIAPEIVS 120
                + + FH +  PL F+++  S +        +A ++   D+GLE+ +LGI+P IV 
Sbjct: 64  -----RRRAFHGSPSPLGFRSTPASWSS------PEAGAAVGGDDGLEVARLGISPWIVE 123

Query: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180
            LA +GIT+LFPIQRAVL+PAMQG+DMIGRARTGTGKTLAFGIPI+D+I++ N K+G GR
Sbjct: 124 RLAARGITRLFPIQRAVLDPAMQGKDMIGRARTGTGKTLAFGIPIMDRILRHNEKNGSGR 183

Query: 181 NPLALVLAPTRELARQVEKEFQEAAPSLDT---------------------ISVGTPGRL 240
           NPLA++LAPTRELARQVEKEF+E+AP LD+                     + VGTPGR+
Sbjct: 184 NPLAIILAPTRELARQVEKEFKESAP-LDSLCVYGGVPISHQMRALNYGVDVVVGTPGRI 243

Query: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRK 300
           IDLL RG LNLSE+QFVVLDEADQML VGF EDVE I+E LPQ RQSM+FSATMPSWIRK
Sbjct: 244 IDLLRRGVLNLSEIQFVVLDEADQMLAVGFDEDVEVIMENLPQNRQSMLFSATMPSWIRK 303

Query: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQ 360
           +T  YL +P+ IDLVGD DQKL +GISL+SIA++ +GK SI+GPLI EHA GGKCIVFTQ
Sbjct: 304 ITSKYLKDPIIIDLVGDEDQKLPEGISLYSIASEHYGKPSILGPLIKEHANGGKCIVFTQ 363

Query: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNV 420
           TKR+ADRLAYAM R++ C+ALHGDISQ+QRERTLSGFRDG FNILVATDVAARGLDIPNV
Sbjct: 364 TKREADRLAYAMGRSYACQALHGDISQNQRERTLSGFRDGRFNILVATDVAARGLDIPNV 423

Query: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRI 480
           DLVIH+ELPN +E+FVHRSGRT RAGKKGSAILIY+ DQ+RAV++IE+++GC+FTELP+I
Sbjct: 424 DLVIHYELPNTSELFVHRSGRTARAGKKGSAILIYTNDQARAVRIIEQDIGCKFTELPKI 483

Query: 481 AVEDGARVDMF------------SDRGGGGQF--GSFGGFRDRRMNDSGRFGG------- 540
            V D A  DMF            S R GG  F  G +GGF + R    G F G       
Sbjct: 484 EVADEAS-DMFNVVRDNRSRLAGSPRTGGSSFGRGGYGGFGEGRSRGFGDFDGFGSSPNR 543

Query: 541 ----QKGGSNFGRSGGYENIKRSSVWDSFGQMGRNNGPSSGGFGSFGGGRSS--GSAFGG 581
               +  GS +G   G+ + +R S  ++FG+   +  P   GFG FG G  S  G+    
Sbjct: 544 GGRSRDAGSRYG--SGFGDFRRPS--NAFGR-SSSKQPDGFGFGDFGEGNFSRNGNRRSR 602

BLAST of Lag0037682 vs. ExPASy Swiss-Prot
Match: A0A1D6LAB7 (DEAD-box ATP-dependent RNA helicase 3B, chloroplastic OS=Zea mays OX=4577 GN=RH3B PE=2 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 4.0e-126
Identity = 240/431 (55.68%), Postives = 307/431 (71.23%), Query Frame = 0

Query: 72  AASGPLNFKASLVSQAEF------AVEEYDDASSSRSADEGLEIG----------KLGIA 131
           AA+G L    S++S+  F      + +E  +      ADEG E G          +LG+ 
Sbjct: 37  AAAGALASPNSVLSEHAFKRLQLGSDDEDGEGPYGSDADEGFEAGEGDNEELAIARLGLP 96

Query: 132 PEIVSALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAK 191
            E+V+ L K+GIT LFPIQRAVL PA++GRD+I RA+TGTGKTLAFGIP++ ++I+ +  
Sbjct: 97  DELVATLEKRGITHLFPIQRAVLIPALEGRDLIARAKTGTGKTLAFGIPMIKQLIEQDDG 156

Query: 192 H--GRGRNPLALVLAPTRELARQVEKEFQEAAPSLDTIS--------------------- 251
               RGR P  LVLAPTRELA+QVEKE +E+AP L T+                      
Sbjct: 157 RITRRGRTPRVLVLAPTRELAKQVEKEIKESAPKLGTVCVYGGVSYNVQQNALSRGVDVV 216

Query: 252 VGTPGRLIDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSAT 311
           VGTPGR+IDL+N GSL L EVQ++VLDEADQML VGF+EDVE IL++LP  RQSM+FSAT
Sbjct: 217 VGTPGRIIDLINGGSLQLGEVQYLVLDEADQMLAVGFEEDVETILQQLPAGRQSMLFSAT 276

Query: 312 MPSWIRKLTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGG 371
           MPSW++KL++ YL NPLTIDLVGD D+KLA+GI L++I      K +++  LIT +AKGG
Sbjct: 277 MPSWVKKLSRRYLNNPLTIDLVGDQDEKLAEGIKLYAIPLTTTSKRTVLSDLITVYAKGG 336

Query: 372 KCIVFTQTKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAAR 431
           K IVFT+TK+DAD ++ A+  +   EALHGDISQ QRERTL+GFR G F +LVATDVAAR
Sbjct: 337 KTIVFTRTKKDADEVSLALTNSIASEALHGDISQHQRERTLNGFRQGKFTVLVATDVAAR 396

Query: 432 GLDIPNVDLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCR 464
           GLDIPNVDL+IH+ELPN+ E FVHRSGRTGRAGK G+AIL+++  Q R VK +ER+VGC 
Sbjct: 397 GLDIPNVDLIIHYELPNDPETFVHRSGRTGRAGKAGTAILMFTSSQKRTVKSLERDVGCN 456

BLAST of Lag0037682 vs. ExPASy TrEMBL
Match: A0A6J1IXE8 (DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480809 PE=4 SV=1)

HSP 1 Score: 948.3 bits (2450), Expect = 1.4e-272
Identity = 529/627 (84.37%), Postives = 550/627 (87.72%), Query Frame = 0

Query: 1   MMNSILLRRSAALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIGVMERP 60
           MM+S+LLRRSAALAASR RVSS  LAPIAN L SSVDVNGGV+PAAEFG FS+ GV ER 
Sbjct: 1   MMSSVLLRRSAALAASRGRVSSTFLAPIANFLTSSVDVNGGVVPAAEFGRFSTTGVTERA 60

Query: 61  LGFGFQSKGFHAASGPLNFKASLVSQAEFAVEEYDDASSSRSADEGLEIGKLGIAPEIVS 120
           LGFGFQS GFHAASGPL+FK+SLVS+AEFAVEEYDDASSS+S DEGLEIG LGIAPEIVS
Sbjct: 61  LGFGFQSMGFHAASGPLSFKSSLVSRAEFAVEEYDDASSSKSGDEGLEIGSLGIAPEIVS 120

Query: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180
           ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR
Sbjct: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180

Query: 181 NPLALVLAPTRELARQVEKEFQEAAPSLDT---------------------ISVGTPGRL 240
            PLALVLAPTRELARQVEKEFQEAAP+LDT                     I+VGTPGRL
Sbjct: 181 YPLALVLAPTRELARQVEKEFQEAAPNLDTICVYGGAPISRQMRQLDYGVDIAVGTPGRL 240

Query: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRK 300
           IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQ+MMFSATMPSWIRK
Sbjct: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQTMMFSATMPSWIRK 300

Query: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQ 360
           LTQNYLTNPLTIDLVGDSDQKLADGISLFSI +DMHGKASIIGPLITEHAKGGKCIVFTQ
Sbjct: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIVSDMHGKASIIGPLITEHAKGGKCIVFTQ 360

Query: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNV 420
           TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFN+LVATDVAARGLDIPNV
Sbjct: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNVLVATDVAARGLDIPNV 420

Query: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRI 480
           DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAV+MIEREVGCRF ELPRI
Sbjct: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVRMIEREVGCRFNELPRI 480

Query: 481 AVEDGARVDMFSDRGGGGQFGSF-GGFRDRRMNDSGRFGGQKGGSNFGRSGGYEN--IKR 540
           +VE GA VDMFS   GGGQF SF GGFRDRRMNDSGRFGGQ+G S FGRS GYE+   +R
Sbjct: 481 SVESGAHVDMFS---GGGQFSSFGGGFRDRRMNDSGRFGGQRGPS-FGRSAGYESSGFRR 540

Query: 541 SSVWDSFGQMGRNNGPSSGGFGSFGGGRSSGSAFG-------GNFGSSGSGRSGGFGDF- 584
           SS  DSFGQ GRN+ PSSGGF SF G RSSGSAFG       G+FGS GSGRSGGFGDF 
Sbjct: 541 SSGRDSFGQGGRNSEPSSGGFSSF-GSRSSGSAFGGSSSKNAGSFGSFGSGRSGGFGDFG 600

BLAST of Lag0037682 vs. ExPASy TrEMBL
Match: A0A6J1J2Y8 (DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480809 PE=4 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.8e-270
Identity = 524/611 (85.76%), Postives = 545/611 (89.20%), Query Frame = 0

Query: 1   MMNSILLRRSAALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIGVMERP 60
           MM+S+LLRRSAALAASR RVSS  LAPIAN L SSVDVNGGV+PAAEFG FS+ GV ER 
Sbjct: 1   MMSSVLLRRSAALAASRGRVSSTFLAPIANFLTSSVDVNGGVVPAAEFGRFSTTGVTERA 60

Query: 61  LGFGFQSKGFHAASGPLNFKASLVSQAEFAVEEYDDASSSRSADEGLEIGKLGIAPEIVS 120
           LGFGFQS GFHAASGPL+FK+SLVS+AEFAVEEYDDASSS+S DEGLEIG LGIAPEIVS
Sbjct: 61  LGFGFQSMGFHAASGPLSFKSSLVSRAEFAVEEYDDASSSKSGDEGLEIGSLGIAPEIVS 120

Query: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180
           ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR
Sbjct: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180

Query: 181 NPLALVLAPTRELARQVEKEFQEAAPSLDT---------------------ISVGTPGRL 240
            PLALVLAPTRELARQVEKEFQEAAP+LDT                     I+VGTPGRL
Sbjct: 181 YPLALVLAPTRELARQVEKEFQEAAPNLDTICVYGGAPISRQMRQLDYGVDIAVGTPGRL 240

Query: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRK 300
           IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQ+MMFSATMPSWIRK
Sbjct: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQTMMFSATMPSWIRK 300

Query: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQ 360
           LTQNYLTNPLTIDLVGDSDQKLADGISLFSI +DMHGKASIIGPLITEHAKGGKCIVFTQ
Sbjct: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIVSDMHGKASIIGPLITEHAKGGKCIVFTQ 360

Query: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNV 420
           TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFN+LVATDVAARGLDIPNV
Sbjct: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNVLVATDVAARGLDIPNV 420

Query: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRI 480
           DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAV+MIEREVGCRF ELPRI
Sbjct: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVRMIEREVGCRFNELPRI 480

Query: 481 AVEDGARVDMFSDRGGGGQFGSF-GGFRDRRMNDSGRFGGQKGGSNFGRSGGYEN--IKR 540
           +VE GA VDMFS   GGGQF SF GGFRDRRMNDSGRFGGQ+G S FGRS GYE+   +R
Sbjct: 481 SVESGAHVDMFS---GGGQFSSFGGGFRDRRMNDSGRFGGQRGPS-FGRSAGYESSGFRR 540

Query: 541 SSVWDSFGQMGRNNGPSSGG---FGSFGGGRSSGSAFGGNFGSSGSGRSGGFGDF-GSGR 584
           SS  DSFGQ GRN+ PSSGG   FGSFG GRS G    G+FGS  S RSGGFGDF GSGR
Sbjct: 541 SSGRDSFGQGGRNSEPSSGGAGSFGSFGSGRSGGF---GDFGS--SSRSGGFGDFGGSGR 600

BLAST of Lag0037682 vs. ExPASy TrEMBL
Match: A0A6J1FJ02 (DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111445803 PE=4 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 6.3e-268
Identity = 519/612 (84.80%), Postives = 541/612 (88.40%), Query Frame = 0

Query: 1   MMNSILLRRSAALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIGVMERP 60
           MM+S+LLRRSAALAASR RVSS  LAPIAN L SSVDVNGG +PAAEFG FS+ GV ER 
Sbjct: 1   MMSSVLLRRSAALAASRGRVSSTFLAPIANFLTSSVDVNGGAVPAAEFGRFSTTGVTERA 60

Query: 61  LGFGFQSKGFHAASGPLNFKASLVSQAEFAVEEYDDASSSRSADEGLEIGKLGIAPEIVS 120
           LGFGFQS GFH+ASGPL FK+SLVS+AEFAVEEYDDASSS+S D+GLEIG LGIAPEIVS
Sbjct: 61  LGFGFQSMGFHSASGPLGFKSSLVSRAEFAVEEYDDASSSKSGDDGLEIGSLGIAPEIVS 120

Query: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180
           ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR
Sbjct: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180

Query: 181 NPLALVLAPTRELARQVEKEFQEAAPSLDT---------------------ISVGTPGRL 240
            PLALVLAPTRELARQVEKEFQEAAP+LDT                     I+VGTPGRL
Sbjct: 181 YPLALVLAPTRELARQVEKEFQEAAPNLDTICVYGGAPISRQMRQLDYGVDIAVGTPGRL 240

Query: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRK 300
           IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQ+MMFSATMPSWIRK
Sbjct: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQTMMFSATMPSWIRK 300

Query: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQ 360
           LTQNYLTNPLTIDLVGDSDQKLADGISLFSI +DM GKASIIGPLITEHAKGGKCIVFTQ
Sbjct: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIVSDMRGKASIIGPLITEHAKGGKCIVFTQ 360

Query: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNV 420
           TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFN+LVATDVAARGLDIPNV
Sbjct: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNVLVATDVAARGLDIPNV 420

Query: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRI 480
           DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAV+MIEREVGCRF ELP+I
Sbjct: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVRMIEREVGCRFNELPKI 480

Query: 481 AVEDGARVDMFSDRGGGGQFGSF--GGFRDRRMNDSGRFGGQKGGSNFGRSGGYEN--IK 540
           +VE GA VDMFS   GGGQF SF  GGFRDRRMNDSGRFGGQ+G S FGRS GYEN   +
Sbjct: 481 SVESGAHVDMFS---GGGQFSSFGGGGFRDRRMNDSGRFGGQRGPS-FGRSAGYENSGFR 540

Query: 541 RSSVWDSFGQMGRNNGPSSGG---FGSFGGGRSSGSAFGGNFGSSGSGRSGGFGDF-GSG 584
           RS   DSFGQ GRN+ PSSGG   FGSFG GRS G    G+FGS  S RSGGFGDF GSG
Sbjct: 541 RSGGRDSFGQGGRNSEPSSGGAGSFGSFGSGRSGGF---GDFGS--SSRSGGFGDFGGSG 600

BLAST of Lag0037682 vs. ExPASy TrEMBL
Match: A0A6J1C209 (DEAD-box ATP-dependent RNA helicase 53-like OS=Momordica charantia OX=3673 GN=LOC111007545 PE=4 SV=1)

HSP 1 Score: 930.2 bits (2403), Expect = 4.1e-267
Identity = 516/616 (83.77%), Postives = 538/616 (87.34%), Query Frame = 0

Query: 1   MMNSILLRRSAALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIGVMERP 60
           MM++++LRRSAALA SR RV++ LL+PIANLLAS VDVNGGV+ AAEFG FSS  VM+RP
Sbjct: 1   MMSALILRRSAALAISRGRVNATLLSPIANLLASPVDVNGGVVSAAEFGQFSSAVVMKRP 60

Query: 61  LGFGFQSKGFHAASGPLNFKASLVSQAEFAVEEYDDASSSRSADEGLEIGKLGIAPEIVS 120
           LGFG + K FH ASGPLNF+ASLVSQAEFAVEEYDDASSSRS DEGLEI KLGIAPEIVS
Sbjct: 61  LGFGSRPKEFHVASGPLNFRASLVSQAEFAVEEYDDASSSRSGDEGLEISKLGIAPEIVS 120

Query: 121 ALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180
           ALAKKGITKLFPIQ+AVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR
Sbjct: 121 ALAKKGITKLFPIQKAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGR 180

Query: 181 NPLALVLAPTRELARQVEKEFQEAAPSLDT---------------------ISVGTPGRL 240
           NPLALVLAPTRELARQVEKEFQEAAPSLDT                     I+VGTPGRL
Sbjct: 181 NPLALVLAPTRELARQVEKEFQEAAPSLDTICVYGGTPISRQMRQLDYGVDIAVGTPGRL 240

Query: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRK 300
           IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLP+KRQSMMFSATMPSWIRK
Sbjct: 241 IDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPKKRQSMMFSATMPSWIRK 300

Query: 301 LTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQ 360
           LTQNYL NPLTIDLVGDSDQKLADGISLFSI ADMHGKASIIGPLI EHAKGGKCIVFTQ
Sbjct: 301 LTQNYLINPLTIDLVGDSDQKLADGISLFSIVADMHGKASIIGPLIKEHAKGGKCIVFTQ 360

Query: 361 TKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNV 420
           TKRDADRLAYAM ++FRCEALHGDISQSQRERTLSGFRDG FNILVATDVAARGLDIPNV
Sbjct: 361 TKRDADRLAYAMPKDFRCEALHGDISQSQRERTLSGFRDGQFNILVATDVAARGLDIPNV 420

Query: 421 DLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRI 480
           DLVIHFELPNN EIFVHRSGRTGRAGKKGSAILIYSQDQSRAV++IEREVGCRFTELPRI
Sbjct: 421 DLVIHFELPNNAEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVRVIEREVGCRFTELPRI 480

Query: 481 AVEDGARVDMFSDRGGGGQFGSFGGFRDRRMNDSGRFGGQKGGSNFGRSGGYEN---IKR 540
            VE GA VDMFSDRG G QFGSFGGFRDRRMND+GRFGGQ+G S FGR GGY N    + 
Sbjct: 481 TVEGGAHVDMFSDRGNGSQFGSFGGFRDRRMNDTGRFGGQRGPS-FGR-GGYGNSGSTRS 540

Query: 541 SSVWDSFGQMGRNNGPSSGGFGSFGGGRS--------SGSAFGGNFGSSG-SGRSGGFGD 584
           S   DSFGQMGRN+G SSGGFGSFG   S        SGS   G FG  G S RSGGFGD
Sbjct: 541 SGFKDSFGQMGRNSGASSGGFGSFGARSSNNAGNFSWSGSGRSGGFGDFGNSSRSGGFGD 600

BLAST of Lag0037682 vs. ExPASy TrEMBL
Match: A0A6J1FFN4 (DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443605 PE=4 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 4.1e-251
Identity = 490/605 (80.99%), Postives = 522/605 (86.28%), Query Frame = 0

Query: 2   MNSILLRRSAALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIGVMERPL 61
           M++ILLRR+ ALAASR RVSS LL+PIAN LAS V VNG VIPAAEFG+FS IGVMERP 
Sbjct: 9   MSAILLRRTTALAASRGRVSSTLLSPIANCLASRVGVNGSVIPAAEFGNFSCIGVMERPS 68

Query: 62  GFGFQSKGFHAASGPLNFKASLVSQAEFAVEEYDDASSSRSADEGLEIGKLGIAPEIVSA 121
           GFGF+S+G HA S PLNF+ASLVS  EFAVEE  +ASSSRS DEGLEIGKLGIAPEIVSA
Sbjct: 69  GFGFRSRGLHAVSRPLNFRASLVSLVEFAVEECVEASSSRSGDEGLEIGKLGIAPEIVSA 128

Query: 122 LAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGRN 181
           L KKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAK G+GRN
Sbjct: 129 LEKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKKGKGRN 188

Query: 182 PLALVLAPTRELARQVEKEFQEAAPSLD---------------------TISVGTPGRLI 241
           PLALVLAPTRELARQVEKEF+EAAPSLD                      I+VGTPGRLI
Sbjct: 189 PLALVLAPTRELARQVEKEFEEAAPSLDMICVYGGTPISSQMRQLGYGVDIAVGTPGRLI 248

Query: 242 DLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRKL 301
           DLLNRGSLNLSEV+FVVLDEADQMLQVGFQEDVEKILERLP+KRQSMMFSATMPSWIRKL
Sbjct: 249 DLLNRGSLNLSEVKFVVLDEADQMLQVGFQEDVEKILERLPRKRQSMMFSATMPSWIRKL 308

Query: 302 TQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQT 361
           +QNYLTNPLT+DLVGDSDQKLADGISLFSI ADM GKASIIGPLI EHAKGGKCIVFTQT
Sbjct: 309 SQNYLTNPLTVDLVGDSDQKLADGISLFSIVADMRGKASIIGPLIAEHAKGGKCIVFTQT 368

Query: 362 KRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNVD 421
           KRDADRLAYAMARNF+CEALHGDISQSQRERTLSGFR G FN+LVATDVAARGLDIPNVD
Sbjct: 369 KRDADRLAYAMARNFKCEALHGDISQSQRERTLSGFRVGRFNVLVATDVAARGLDIPNVD 428

Query: 422 LVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRIA 481
           LVIHFELPNNTEIFVHRSGRTGRAGKKGSAILI++Q+QSRAV++IEREVGCRF ELPRI 
Sbjct: 429 LVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIHTQNQSRAVRVIEREVGCRFNELPRIT 488

Query: 482 VEDGARVDMFSDRGGGGQFGSFGGFRDRRMNDSGRFGGQKGGSNFGRSGGYEN--IKRSS 541
           VEDGA VDMFS+RG GG+F SFGGF+D R NDSGR GGQ+G S F RSG YEN    RSS
Sbjct: 489 VEDGAHVDMFSERGVGGRFSSFGGFKDPRTNDSGRTGGQRGPS-FRRSGDYENSSFGRSS 548

Query: 542 VWDSFGQMGRNNGPSSGGFGSFGGGRSSGSAFGGNFGSSGSGRSGGFG--DFGSGRSSGF 582
             DSFG  GRN G +SGGF SFG  +S+ +   G+F SSGS RSGGFG  DF SGRSSG 
Sbjct: 549 GRDSFGPKGRNGGHTSGGFSSFGARKSNNA---GDFRSSGSRRSGGFGDLDFDSGRSSGR 608

BLAST of Lag0037682 vs. TAIR 10
Match: AT3G22330.1 (putative mitochondrial RNA helicase 2 )

HSP 1 Score: 678.3 bits (1749), Expect = 5.4e-195
Identity = 397/607 (65.40%), Postives = 450/607 (74.14%), Query Frame = 0

Query: 22  SALLAPIANLLASSVDVN----GGVIPAA-EFGHFSSIGVMERPLG--FGFQSKGFHAAS 81
           S L A   NL AS   +N      + PAA      + IG  +   G  FG ++KG H  S
Sbjct: 9   SLLDASKRNLSASLTSINTVLFHNLAPAATRVSDLALIGSSDVKAGFPFGVEAKGIHFQS 68

Query: 82  GPLNFKASLVSQAEFAVEEYDD---ASSSRSADEGLEIGKLGIAPEIVSALAKKGITKLF 141
           GPL+F+AS+VSQA FA+ E  +     S     +GL I +LGI+PEIV AL+ KGI KLF
Sbjct: 69  GPLDFRASMVSQAGFAISESSERRVGDSESVGGDGLAISELGISPEIVKALSSKGIEKLF 128

Query: 142 PIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQFNAKHGRGRNPLALVLAPTR 201
           PIQ+AVLEPAM+GRDMIGRARTGTGKTLAFGIPI+DKII++NAKHGRGRNPL LVLAPTR
Sbjct: 129 PIQKAVLEPAMEGRDMIGRARTGTGKTLAFGIPIIDKIIKYNAKHGRGRNPLCLVLAPTR 188

Query: 202 ELARQVEKEFQEAAPSLDTI---------------------SVGTPGRLIDLLNRGSLNL 261
           ELARQVEKEF+E+APSLDTI                     +VGTPGR+IDL+ RG+LNL
Sbjct: 189 ELARQVEKEFRESAPSLDTICLYGGTPIGQQMRQLDYGVDVAVGTPGRVIDLMKRGALNL 248

Query: 262 SEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRKLTQNYLTNPLT 321
           SEVQFVVLDEADQMLQVGF EDVE ILE+LP+KRQSMMFSATMPSWIR LT+ YL NPLT
Sbjct: 249 SEVQFVVLDEADQMLQVGFAEDVEIILEKLPEKRQSMMFSATMPSWIRSLTKKYLNNPLT 308

Query: 322 IDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQTKRDADRLAYA 381
           +DLVGDSDQKLADGI+ +SI AD +G+ASIIGPL+TEHAKGGKCIVFTQTKRDADRL+YA
Sbjct: 309 VDLVGDSDQKLADGITTYSIIADSYGRASIIGPLVTEHAKGGKCIVFTQTKRDADRLSYA 368

Query: 382 MARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNVDLVIHFELPNN 441
           +AR+F+CEALHGDISQSQRERTL+GFRDGHFNILVATDVAARGLD+PNVDL+IH+ELPNN
Sbjct: 369 LARSFKCEALHGDISQSQRERTLAGFRDGHFNILVATDVAARGLDVPNVDLIIHYELPNN 428

Query: 442 TEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRFTELPRIAVEDGARVDMF 501
           TE FVHR+GRTGRAGKKGSAILIYSQDQSRAVK+IEREVG RFTELP IAVE G+   MF
Sbjct: 429 TETFVHRTGRTGRAGKKGSAILIYSQDQSRAVKIIEREVGSRFTELPSIAVERGS-ASMF 488

Query: 502 SDRG--GGGQFGSFGGFRDRRMNDSGR-----FGGQKGGSNFGRSGG----YENIKRSSV 561
              G   GG FG  GG RDR  +  GR     +GG  GG   GRSGG    Y      S 
Sbjct: 489 EGIGSRSGGSFG--GGMRDRGSSFGGRSGGGGYGGSSGGYGGGRSGGSSNRYSGDSDRSG 548

Query: 562 WDSFGQ-----MGRNNGPSSGGFGSFGGGRSSGSAFGGNFGSSGSGRSGGFGDFGSGRSS 582
           + SFG       G +    SGG  SFGGGRS GS+         + RS GFGDFGS RSS
Sbjct: 549 FGSFGMRSPEGYGSDRSSQSGGRSSFGGGRSGGSS---------NNRSSGFGDFGSDRSS 603

BLAST of Lag0037682 vs. TAIR 10
Match: AT3G22310.1 (putative mitochondrial RNA helicase 1 )

HSP 1 Score: 629.4 bits (1622), Expect = 2.9e-180
Identity = 381/623 (61.16%), Postives = 446/623 (71.59%), Query Frame = 0

Query: 2   MNSILLRRS------AALAASRARVSSALLAPIANLLASSVDVNGGVIPAAEFGHFSSIG 61
           M S +LRRS        LAAS   +++AL   +A   A+  D+  G          SS  
Sbjct: 1   MISTVLRRSILGTSRRTLAASVTSINAALFHNLAPAAATVSDLANGATNVKSLPSNSS-- 60

Query: 62  VMERPLGFGFQSKGFHAASGPLNFKASLVSQAEFAVEEY------------DDASSSRSA 121
                  FG + + FH  S P  F++S+VS A FA +EY             ++  S   
Sbjct: 61  ------PFGVKVRDFHVKSVPSEFRSSIVSSAGFAAQEYAPSYENDGGIGDSESVGSSGG 120

Query: 122 DEGLEIGKLGIAPEIVSALAKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGI 181
            +GL I  LGI+PEIV AL  +GI KLFPIQ+AVLEPAM+GRDMIGRARTGTGKTLAFGI
Sbjct: 121 GDGLAIADLGISPEIVKALKGRGIEKLFPIQKAVLEPAMEGRDMIGRARTGTGKTLAFGI 180

Query: 182 PILDKIIQFNAKHGRGRNPLALVLAPTRELARQVEKEFQEAAPSLDTI------------ 241
           PI+DKII+FNAKHGRG+NP  LVLAPTRELARQVEKEF+E+APSLDTI            
Sbjct: 181 PIIDKIIKFNAKHGRGKNPQCLVLAPTRELARQVEKEFRESAPSLDTICLYGGTPIGQQM 240

Query: 242 ---------SVGTPGRLIDLLNRGSLNLSEVQFVVLDEADQMLQVGFQEDVEKILERLPQ 301
                    +VGTPGR+IDL+ RG+LNLSEVQFVVLDEADQMLQVGF EDVE IL++LP 
Sbjct: 241 RELNYGIDVAVGTPGRIIDLMKRGALNLSEVQFVVLDEADQMLQVGFAEDVEIILQKLPA 300

Query: 302 KRQSMMFSATMPSWIRKLTQNYLTNPLTIDLVGDSDQKLADGISLFSIAADMHGKASIIG 361
           KRQSMMFSATMPSWIR LT+ YL NPLTIDLVGDSDQKLADGI+++SIAAD +G+ASIIG
Sbjct: 301 KRQSMMFSATMPSWIRSLTKKYLNNPLTIDLVGDSDQKLADGITMYSIAADSYGRASIIG 360

Query: 362 PLITEHAKGGKCIVFTQTKRDADRLAYAMARNFRCEALHGDISQSQRERTLSGFRDGHFN 421
           PL+ EH KGGKCIVFTQTKRDADRLA+ +A++++CEALHGDISQ+QRERTL+GFRDG+F+
Sbjct: 361 PLVKEHGKGGKCIVFTQTKRDADRLAFGLAKSYKCEALHGDISQAQRERTLAGFRDGNFS 420

Query: 422 ILVATDVAARGLDIPNVDLVIHFELPNNTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAV 481
           ILVATDVAARGLD+PNVDLVIH+ELPNNTE FVHR+GRTGRAGKKGSAILI+ QDQ+RAV
Sbjct: 421 ILVATDVAARGLDVPNVDLVIHYELPNNTETFVHRTGRTGRAGKKGSAILIHGQDQTRAV 480

Query: 482 KMIEREVGCRFTELPRIAVEDGARVDMFSDRGGGGQFGSFGGFRDRRMNDSGRFGGQKGG 541
           KMIE+EVG RF ELP IAVE G+   MF +  G    GSFGG R      SG  G    G
Sbjct: 481 KMIEKEVGSRFNELPSIAVERGS-ASMF-EGVGARSGGSFGGGR------SGGGGYGSYG 540

Query: 542 SNFGRSGGYENIKRSSVWDSFGQMGRNNGPSSGGFGSFGGGRSSGSAFGGNFGSSGSGRS 584
           S+ GRSGG           S+G  G ++G S GG GS+GG   S S + G     GS RS
Sbjct: 541 SSSGRSGG----------GSYGGYGGSSGRSGGGGGSYGGSGGSSSRYSG-----GSDRS 592

BLAST of Lag0037682 vs. TAIR 10
Match: AT5G26742.1 (DEAD box RNA helicase (RH3) )

HSP 1 Score: 438.7 bits (1127), Expect = 7.2e-123
Identity = 229/388 (59.02%), Postives = 285/388 (73.45%), Query Frame = 0

Query: 93  EYDDASSSRSADEG--LEIGKLGIAPEIVSALAKKGITKLFPIQRAVLEPAMQGRDMIGR 152
           + D  +++  AD+G  L I KL +   +  +L K+GIT LFPIQRAVL PA+QGRD+I R
Sbjct: 86  DLDGDNNNVEADDGEELAISKLSLPQRLEESLEKRGITHLFPIQRAVLVPALQGRDIIAR 145

Query: 153 ARTGTGKTLAFGIPILDKIIQ----FNAKHGRGRNPLALVLAPTRELARQVEKEFQEAAP 212
           A+TGTGKTLAFGIPI+ ++ +    + A    GR P  LVLAPTRELA+QVEKE +E+AP
Sbjct: 146 AKTGTGKTLAFGIPIIKRLTEEAGDYTAFRRSGRLPKFLVLAPTRELAKQVEKEIKESAP 205

Query: 213 SLDTIS---------------------VGTPGRLIDLLNRGSLNLSEVQFVVLDEADQML 272
            L T+                      VGTPGR+IDL+   SL L EV+++VLDEADQML
Sbjct: 206 YLSTVCVYGGVSYTIQQSALTRGVDVVVGTPGRIIDLIEGRSLKLGEVEYLVLDEADQML 265

Query: 273 QVGFQEDVEKILERLPQKRQSMMFSATMPSWIRKLTQNYLTNPLTIDLVGDSDQKLADGI 332
            VGF+E VE ILE LP KRQSM+FSATMP+W++KL + YL NPL IDLVGD D+KLA+GI
Sbjct: 266 AVGFEEAVESILENLPTKRQSMLFSATMPTWVKKLARKYLDNPLNIDLVGDQDEKLAEGI 325

Query: 333 SLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQTKRDADRLAYAMARNFRCEALHGDIS 392
            L++IA     K +I+  LIT +AKGGK IVFTQTKRDAD ++ A++ +   EALHGDIS
Sbjct: 326 KLYAIATTSTSKRTILSDLITVYAKGGKTIVFTQTKRDADEVSLALSNSIATEALHGDIS 385

Query: 393 QSQRERTLSGFRDGHFNILVATDVAARGLDIPNVDLVIHFELPNNTEIFVHRSGRTGRAG 452
           Q QRERTL+ FR G F +LVATDVA+RGLDIPNVDLVIH+ELPN+ E FVHRSGRTGRAG
Sbjct: 386 QHQRERTLNAFRQGKFTVLVATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAG 445

Query: 453 KKGSAILIYSQDQSRAVKMIEREVGCRF 454
           K+GSAIL+++  Q R V+ +ER+VGC F
Sbjct: 446 KEGSAILMHTSSQKRTVRSLERDVGCHF 473

BLAST of Lag0037682 vs. TAIR 10
Match: AT5G26742.2 (DEAD box RNA helicase (RH3) )

HSP 1 Score: 438.7 bits (1127), Expect = 7.2e-123
Identity = 229/388 (59.02%), Postives = 285/388 (73.45%), Query Frame = 0

Query: 93  EYDDASSSRSADEG--LEIGKLGIAPEIVSALAKKGITKLFPIQRAVLEPAMQGRDMIGR 152
           + D  +++  AD+G  L I KL +   +  +L K+GIT LFPIQRAVL PA+QGRD+I R
Sbjct: 86  DLDGDNNNVEADDGEELAISKLSLPQRLEESLEKRGITHLFPIQRAVLVPALQGRDIIAR 145

Query: 153 ARTGTGKTLAFGIPILDKIIQ----FNAKHGRGRNPLALVLAPTRELARQVEKEFQEAAP 212
           A+TGTGKTLAFGIPI+ ++ +    + A    GR P  LVLAPTRELA+QVEKE +E+AP
Sbjct: 146 AKTGTGKTLAFGIPIIKRLTEEAGDYTAFRRSGRLPKFLVLAPTRELAKQVEKEIKESAP 205

Query: 213 SLDTIS---------------------VGTPGRLIDLLNRGSLNLSEVQFVVLDEADQML 272
            L T+                      VGTPGR+IDL+   SL L EV+++VLDEADQML
Sbjct: 206 YLSTVCVYGGVSYTIQQSALTRGVDVVVGTPGRIIDLIEGRSLKLGEVEYLVLDEADQML 265

Query: 273 QVGFQEDVEKILERLPQKRQSMMFSATMPSWIRKLTQNYLTNPLTIDLVGDSDQKLADGI 332
            VGF+E VE ILE LP KRQSM+FSATMP+W++KL + YL NPL IDLVGD D+KLA+GI
Sbjct: 266 AVGFEEAVESILENLPTKRQSMLFSATMPTWVKKLARKYLDNPLNIDLVGDQDEKLAEGI 325

Query: 333 SLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQTKRDADRLAYAMARNFRCEALHGDIS 392
            L++IA     K +I+  LIT +AKGGK IVFTQTKRDAD ++ A++ +   EALHGDIS
Sbjct: 326 KLYAIATTSTSKRTILSDLITVYAKGGKTIVFTQTKRDADEVSLALSNSIATEALHGDIS 385

Query: 393 QSQRERTLSGFRDGHFNILVATDVAARGLDIPNVDLVIHFELPNNTEIFVHRSGRTGRAG 452
           Q QRERTL+ FR G F +LVATDVA+RGLDIPNVDLVIH+ELPN+ E FVHRSGRTGRAG
Sbjct: 386 QHQRERTLNAFRQGKFTVLVATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAG 445

Query: 453 KKGSAILIYSQDQSRAVKMIEREVGCRF 454
           K+GSAIL+++  Q R V+ +ER+VGC F
Sbjct: 446 KEGSAILMHTSSQKRTVRSLERDVGCHF 473

BLAST of Lag0037682 vs. TAIR 10
Match: AT5G26742.3 (DEAD box RNA helicase (RH3) )

HSP 1 Score: 411.0 bits (1055), Expect = 1.6e-114
Identity = 211/344 (61.34%), Postives = 258/344 (75.00%), Query Frame = 0

Query: 135 RAVLEPAMQGRDMIGRARTGTGKTLAFGIPILDKIIQ----FNAKHGRGRNPLALVLAPT 194
           RAVL PA+QGRD+I RA+TGTGKTLAFGIPI+ ++ +    + A    GR P  LVLAPT
Sbjct: 37  RAVLVPALQGRDIIARAKTGTGKTLAFGIPIIKRLTEEAGDYTAFRRSGRLPKFLVLAPT 96

Query: 195 RELARQVEKEFQEAAPSLDTIS---------------------VGTPGRLIDLLNRGSLN 254
           RELA+QVEKE +E+AP L T+                      VGTPGR+IDL+   SL 
Sbjct: 97  RELAKQVEKEIKESAPYLSTVCVYGGVSYTIQQSALTRGVDVVVGTPGRIIDLIEGRSLK 156

Query: 255 LSEVQFVVLDEADQMLQVGFQEDVEKILERLPQKRQSMMFSATMPSWIRKLTQNYLTNPL 314
           L EV+++VLDEADQML VGF+E VE ILE LP KRQSM+FSATMP+W++KL + YL NPL
Sbjct: 157 LGEVEYLVLDEADQMLAVGFEEAVESILENLPTKRQSMLFSATMPTWVKKLARKYLDNPL 216

Query: 315 TIDLVGDSDQKLADGISLFSIAADMHGKASIIGPLITEHAKGGKCIVFTQTKRDADRLAY 374
            IDLVGD D+KLA+GI L++IA     K +I+  LIT +AKGGK IVFTQTKRDAD ++ 
Sbjct: 217 NIDLVGDQDEKLAEGIKLYAIATTSTSKRTILSDLITVYAKGGKTIVFTQTKRDADEVSL 276

Query: 375 AMARNFRCEALHGDISQSQRERTLSGFRDGHFNILVATDVAARGLDIPNVDLVIHFELPN 434
           A++ +   EALHGDISQ QRERTL+ FR G F +LVATDVA+RGLDIPNVDLVIH+ELPN
Sbjct: 277 ALSNSIATEALHGDISQHQRERTLNAFRQGKFTVLVATDVASRGLDIPNVDLVIHYELPN 336

Query: 435 NTEIFVHRSGRTGRAGKKGSAILIYSQDQSRAVKMIEREVGCRF 454
           + E FVHRSGRTGRAGK+GSAIL+++  Q R V+ +ER+VGC F
Sbjct: 337 DPETFVHRSGRTGRAGKEGSAILMHTSSQKRTVRSLERDVGCHF 380

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022981751.13.0e-27284.37DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like isoform X1 [Cucurbita... [more]
XP_023524742.12.8e-27083.60DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like [Cucurbita pepo subsp... [more]
KAG7037512.13.7e-27083.60DEAD-box ATP-dependent RNA helicase 53 [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_022981753.13.7e-27085.76DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like isoform X2 [Cucurbita... [more]
XP_022940069.11.3e-26784.80DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LUW57.6e-19465.40DEAD-box ATP-dependent RNA helicase 53, mitochondrial OS=Arabidopsis thaliana OX... [more]
Q9LUW64.0e-17961.16DEAD-box ATP-dependent RNA helicase 9, mitochondrial OS=Arabidopsis thaliana OX=... [more]
Q0ILZ47.6e-16256.64DEAD-box ATP-dependent RNA helicase 9 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q0D8N01.2e-15955.38DEAD-box ATP-dependent RNA helicase 53 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
A0A1D6LAB74.0e-12655.68DEAD-box ATP-dependent RNA helicase 3B, chloroplastic OS=Zea mays OX=4577 GN=RH3... [more]
Match NameE-valueIdentityDescription
A0A6J1IXE81.4e-27284.37DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like isoform X1 OS=Cucurbi... [more]
A0A6J1J2Y81.8e-27085.76DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like isoform X2 OS=Cucurbi... [more]
A0A6J1FJ026.3e-26884.80DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like OS=Cucurbita moschata... [more]
A0A6J1C2094.1e-26783.77DEAD-box ATP-dependent RNA helicase 53-like OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1FFN44.1e-25180.99DEAD-box ATP-dependent RNA helicase 53, mitochondrial-like isoform X2 OS=Cucurbi... [more]
Match NameE-valueIdentityDescription
AT3G22330.15.4e-19565.40putative mitochondrial RNA helicase 2 [more]
AT3G22310.12.9e-18061.16putative mitochondrial RNA helicase 1 [more]
AT5G26742.17.2e-12359.02DEAD box RNA helicase (RH3) [more]
AT5G26742.27.2e-12359.02DEAD box RNA helicase (RH3) [more]
AT5G26742.31.6e-11461.34DEAD box RNA helicase (RH3) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 126..308
e-value: 3.7E-47
score: 172.6
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 138..291
score: 26.560816
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 345..425
e-value: 7.6E-32
score: 121.8
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 318..425
e-value: 2.5E-31
score: 108.3
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 320..465
score: 22.832943
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 81..210
e-value: 3.9E-32
score: 113.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 311..470
e-value: 4.0E-51
score: 175.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 211..295
e-value: 2.8E-30
score: 107.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 181..447
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 131..205
e-value: 3.4E-17
score: 62.8
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 211..279
e-value: 5.0E-20
score: 72.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 522..583
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 522..541
NoneNo IPR availablePANTHERPTHR47958:SF99DEAD-BOX ATP-DEPENDENT RNA HELICASE 53-LIKEcoord: 60..518
NoneNo IPR availablePANTHERPTHR47958ATP-DEPENDENT RNA HELICASE DBP3coord: 60..518
NoneNo IPR availableCDDcd18787SF2_C_DEADcoord: 317..434
e-value: 5.56957E-54
score: 177.699
NoneNo IPR availableCDDcd00268DEADccoord: 118..292
e-value: 5.3226E-84
score: 258.526

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0037682.1Lag0037682.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000373 Group II intron splicing
biological_process GO:0006364 rRNA processing
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003724 RNA helicase activity