Lag0037578 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0037578
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	3-hydroxy-3-methylglutaryl-coenzyme A reductase
Location	chr2: 7397479 .. 7398251 (+)
RNA-Seq Expression	Lag0037578
Synteny	Lag0037578

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGCGTGTTGAATCTTCTGCTTCGTGTTCTGATTTTCGATTTACTCATGCGCTCGCTCGCGCCATTTCTCCTCCTGAGCTTTTCTCTCGGGTTTGGGCTAGGGCTTCATTGTTGTTACCATGATTCAATGTGATACTGCCGATTCGAGTGGTACAATTTGCTTCCTGTCAATTTAATGTTACTCAGATTTCATATATATTGCGCTGGAGATATTTATATCCTATTTTGCAGATGATTTGAGATCAGAAAAGTCTCGGAGTATTATATTTTCTTACTATTTCGTCGTCTTTGCCGATAAGAGCACATCGGTGTTTTTGAATCATATGCATTCGCAACAGAGTTATTGGGCTAAAATCCTTGTCTGTTTTAACCATTCTCGCTACTCTGTAAATGTTTGGTTAGAAAGGACGAGAAGCTTATCATTATTTTCGGAAACTGAAGGTGTCCTTACATTGCTTACATTTCGCACAACATGGTCTCTAAAGGCGTGCAGAACGTTCTCGATTTCTTACAGGAGGATTTCCCTGACATGGATGTAATCGGCATTTCCGGTAAGTTAATAGTACAAGAAAATTACATCCTGTTTCTATATATATATTTTTTAAATAGTACAACAAAATTACAACAACTTATTCGATTTCATTCCCAAAATCATTTCAAAATCATTTGATTGTGGTTGATTAGGAAATTTCTGCTTGGATAAGAAACCGGCTGCTGTGAACTGGGTTGAAGGAAGGGTGAAGTACGTGGTTTGTGAGGCCATAATTTAA

mRNA sequence

ATGGTGCGTGTTGAATCTTCTGCTTCGTGTTCTGATTTTCGATTTACTCATGCGCTCGCTCGCGCCATTTCTCCTCCTGAGCTTTTCTCTCGGAACGTTCTCGATTTCTTACAGGAGGATTTCCCTGACATGGATGTAATCGGCATTTCCGGAAATTTCTGCTTGGATAAGAAACCGGCTGCTGTGAACTGGGTTGAAGGAAGGGTGAAGTACGTGGTTTGTGAGGCCATAATTTAA

Coding sequence (CDS)

ATGGTGCGTGTTGAATCTTCTGCTTCGTGTTCTGATTTTCGATTTACTCATGCGCTCGCTCGCGCCATTTCTCCTCCTGAGCTTTTCTCTCGGAACGTTCTCGATTTCTTACAGGAGGATTTCCCTGACATGGATGTAATCGGCATTTCCGGAAATTTCTGCTTGGATAAGAAACCGGCTGCTGTGAACTGGGTTGAAGGAAGGGTGAAGTACGTGGTTTGTGAGGCCATAATTTAA

Protein sequence

MVRVESSASCSDFRFTHALARAISPPELFSRNVLDFLQEDFPDMDVIGISGNFCLDKKPAAVNWVEGRVKYVVCEAII

Homology

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BLAST of Lag0037578 vs. NCBI nr
Match: TKY57061.1 (3-hydroxy-3-methylglutaryl-coenzyme A reductase [Spatholobus suberectus])

HSP 1 Score: 95.5 bits (236), Expect = 2.1e-16
Identity = 48/76 (63.16%), Postives = 53/76 (69.74%), Query Frame = 0


Query: 6   SSASCSDFRFTHALARAISPPELFS---RNVLDFLQEDFPDMDVIGISGNFCLDKKPAAV 65
           SSAS  D     A+    +P        +NVLDFLQ DFPDMDVIGISGN+C DKKPAAV
Sbjct: 129 SSASGDDEEIVSAVVEGATPSHALEGELKNVLDFLQNDFPDMDVIGISGNYCSDKKPAAV 188

Query: 66  NWVEGRVKYVVCEAII 79
           NW+EGR K VVCEAII
Sbjct: 189 NWIEGRGKSVVCEAII 204

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BLAST of Lag0037578 vs. NCBI nr
Match: XP_023529829.1 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 95.1 bits (235), Expect = 2.8e-16
Identity = 46/65 (70.77%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 14  RFTHALARAISPPELFSRNVLDFLQEDFPDMDVIGISGNFCLDKKPAAVNWVEGRVKYVV 73
           RF+ +   A+    +   NVLDFLQEDFPDMDVIGISGNFC DKKPAAVNW+EGR K VV
Sbjct: 348 RFSCSTGDAMGMNMVSKGNVLDFLQEDFPDMDVIGISGNFCSDKKPAAVNWIEGRGKSVV 407

Query: 74  CEAII 79
           CEAII
Sbjct: 408 CEAII 412

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BLAST of Lag0037578 vs. NCBI nr
Match: XP_022928221.1 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Cucurbita moschata] >XP_022928223.1 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Cucurbita moschata])

HSP 1 Score: 94.0 bits (232), Expect = 6.2e-16
Identity = 43/48 (89.58%), Postives = 45/48 (93.75%), Query Frame = 0


Query: 31  RNVLDFLQEDFPDMDVIGISGNFCLDKKPAAVNWVEGRVKYVVCEAII 79
           +NVLDFLQEDFPDMDVIGISGNFC DKKPAAVNW+EGR K VVCEAII
Sbjct: 363 QNVLDFLQEDFPDMDVIGISGNFCSDKKPAAVNWIEGRGKSVVCEAII 410

[Close]

BLAST of Lag0037578 vs. NCBI nr
Match: XP_022989367.1 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Cucurbita maxima] >XP_022989368.1 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Cucurbita maxima])

HSP 1 Score: 94.0 bits (232), Expect = 6.2e-16
Identity = 43/48 (89.58%), Postives = 45/48 (93.75%), Query Frame = 0


Query: 31  RNVLDFLQEDFPDMDVIGISGNFCLDKKPAAVNWVEGRVKYVVCEAII 79
           +NVLDFLQEDFPDMDVIGISGNFC DKKPAAVNW+EGR K VVCEAII
Sbjct: 367 QNVLDFLQEDFPDMDVIGISGNFCSDKKPAAVNWIEGRGKSVVCEAII 414

[Close]

BLAST of Lag0037578 vs. NCBI nr
Match: XP_038888927.1 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Benincasa hispida])

HSP 1 Score: 94.0 bits (232), Expect = 6.2e-16
Identity = 43/48 (89.58%), Postives = 45/48 (93.75%), Query Frame = 0


Query: 31  RNVLDFLQEDFPDMDVIGISGNFCLDKKPAAVNWVEGRVKYVVCEAII 79
           +NVLDFLQEDFPDMDVIGISGNFC DKKPAAVNW+EGR K VVCEAII
Sbjct: 366 QNVLDFLQEDFPDMDVIGISGNFCSDKKPAAVNWIEGRGKSVVCEAII 413

[Close]

BLAST of Lag0037578 vs. ExPASy Swiss-Prot
Match: O64966 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 OS=Gossypium hirsutum OX=3635 GN=HMG1 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 4.1e-18
Identity = 42/48 (87.50%), Postives = 44/48 (91.67%), Query Frame = 0


Query: 31  RNVLDFLQEDFPDMDVIGISGNFCLDKKPAAVNWVEGRVKYVVCEAII 79
           +NVLDFLQ DFPDMDVIGISGNFC DKKPAAVNW+EGR K VVCEAII
Sbjct: 370 QNVLDFLQTDFPDMDVIGISGNFCSDKKPAAVNWIEGRGKSVVCEAII 417

[Close]

BLAST of Lag0037578 vs. ExPASy Swiss-Prot
Match: O64967 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 2 OS=Gossypium hirsutum OX=3635 GN=HMG2 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 4.1e-18
Identity = 42/48 (87.50%), Postives = 44/48 (91.67%), Query Frame = 0


Query: 31  RNVLDFLQEDFPDMDVIGISGNFCLDKKPAAVNWVEGRVKYVVCEAII 79
           +NVLDFLQ DFPDMDVIGISGNFC DKKPAAVNW+EGR K VVCEAII
Sbjct: 413 QNVLDFLQTDFPDMDVIGISGNFCSDKKPAAVNWIEGRGKSVVCEAII 460

[Close]

BLAST of Lag0037578 vs. ExPASy Swiss-Prot
Match: P48021 (3-hydroxy-3-methylglutaryl-coenzyme A reductase OS=Camptotheca acuminata OX=16922 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 5.3e-18
Identity = 40/48 (83.33%), Postives = 45/48 (93.75%), Query Frame = 0


Query: 31  RNVLDFLQEDFPDMDVIGISGNFCLDKKPAAVNWVEGRVKYVVCEAII 79
           +NVLDFLQ+DFPDMDVIGISGN+C DKKPAAVNW+EGR K VVCEA+I
Sbjct: 378 QNVLDFLQDDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEAVI 425

[Close]

BLAST of Lag0037578 vs. ExPASy Swiss-Prot
Match: A0A0A1C3I2 (3-hydroxy-3-methylglutaryl coenzyme A reductase 1 OS=Panax ginseng OX=4054 GN=HMGR1 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 9.0e-18
Identity = 41/48 (85.42%), Postives = 44/48 (91.67%), Query Frame = 0


Query: 31  RNVLDFLQEDFPDMDVIGISGNFCLDKKPAAVNWVEGRVKYVVCEAII 79
           +NVL+FLQ DFPDMDVIGISGNFC DKKPAAVNW+EGR K VVCEAII
Sbjct: 358 QNVLEFLQSDFPDMDVIGISGNFCSDKKPAAVNWIEGRGKSVVCEAII 405

[Close]

BLAST of Lag0037578 vs. ExPASy Swiss-Prot
Match: U5JCC6 (3-hydroxy-3-methylglutaryl coenzyme A reductase 2-B OS=Panax ginseng OX=4054 GN=HMGR2 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.5e-17
Identity = 40/48 (83.33%), Postives = 44/48 (91.67%), Query Frame = 0


Query: 31  RNVLDFLQEDFPDMDVIGISGNFCLDKKPAAVNWVEGRVKYVVCEAII 79
           +NVLDFLQ DFPDMDV+GISGN+C DKKPAAVNW+EGR K VVCEAII
Sbjct: 374 QNVLDFLQNDFPDMDVMGISGNYCSDKKPAAVNWIEGRGKSVVCEAII 421

The following BLAST results are available for this feature:

BLAST of Lag0037578 vs. NCBI nr


Match Name	E-value	Identity	Description
TKY57061.1	2.1e-16	63.16	3-hydroxy-3-methylglutaryl-coenzyme A reductase [Spatholobus suberectus]	[more]
XP_023529829.1	2.8e-16	70.77	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Cucurbita pepo subsp. pe...	[more]
XP_022928221.1	6.2e-16	89.58	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Cucurbita moschata] >XP_...	[more]
XP_022989367.1	6.2e-16	89.58	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Cucurbita maxima] >XP_02...	[more]
XP_038888927.1	6.2e-16	89.58	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Benincasa hispida]	[more]

BLAST of Lag0037578 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64966	4.1e-18	87.50	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 OS=Gossypium hirsutum OX=3635 ...	[more]
O64967	4.1e-18	87.50	3-hydroxy-3-methylglutaryl-coenzyme A reductase 2 OS=Gossypium hirsutum OX=3635 ...	[more]
P48021	5.3e-18	83.33	3-hydroxy-3-methylglutaryl-coenzyme A reductase OS=Camptotheca acuminata OX=1692...	[more]
A0A0A1C3I2	9.0e-18	85.42	3-hydroxy-3-methylglutaryl coenzyme A reductase 1 OS=Panax ginseng OX=4054 GN=HM...	[more]
U5JCC6	1.5e-17	83.33	3-hydroxy-3-methylglutaryl coenzyme A reductase 2-B OS=Panax ginseng OX=4054 GN=...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009023	Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain superfamily	GENE3D	3.30.70.420		coord: 27..68 e-value: 1.2E-13 score: 53.6
IPR009023	Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain superfamily	SUPERFAMILY	55035	NAD-binding domain of HMG-CoA reductase	coord: 31..68
IPR002202	Hydroxymethylglutaryl-CoA reductase, class I/II	PFAM	PF00368	HMG-CoA_red	coord: 31..78 e-value: 2.6E-9 score: 36.5
IPR002202	Hydroxymethylglutaryl-CoA reductase, class I/II	PANTHER	PTHR10572	3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE	coord: 31..78
IPR002202	Hydroxymethylglutaryl-CoA reductase, class I/II	PROSITE	PS50065	HMG_COA_REDUCTASE_4	coord: 30..78 score: 16.692902
None	No IPR available	PANTHER	PTHR10572:SF30	3-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASE	coord: 31..78

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0037578.1	Lag0037578.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015936	coenzyme A metabolic process
biological_process	GO:0009646	response to absence of light
biological_process	GO:0009753	response to jasmonic acid
biological_process	GO:0016135	saponin biosynthetic process
biological_process	GO:0016104	triterpenoid biosynthetic process
cellular_component	GO:0031969	chloroplast membrane
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005778	peroxisomal membrane
molecular_function	GO:0004420	hydroxymethylglutaryl-CoA reductase (NADPH) activity