Lag0037436 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0037436
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRhamnogalacturonan endolyase
Locationchr2: 6247878 .. 6252909 (-)
RNA-Seq ExpressionLag0037436
SyntenyLag0037436
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGTCCACGGGAGTGCGATTGCAGATACGAGATCGTCATGTAAGTTAGCTTTTCCATTTTGTTCTGATTCTGTGAAGATATTCCAGAAGAAAATCACGAAAGGGAAGACTATGTATAATTGAAGATTGAAGAGAAGAAATGTTGTAATTTCCTTCCAGAACTCTGCAGATTATTCCTTGTTTATTCACGTTTTGTGCAGGTGGTGGTGGATAATGGAATACTGCAACTAACTTTATCAAAACCAGATGGAATCATCACTGGCATACGATATAATGGTGTTGACAATCTGCTTGAAGTTCTCAACCCTGAATCTAACAGAGGGTATACGCTGTTATAATCGTCATACCTCTGAAATTAATTTAATTAACAAAGAGTTGAAGATGACAATCTGCTTGTTTTTGTTATGAACATTCAGTTATTGGGATCTCGTTTGGAATTCTCCAGGAAGCAAGGGAATATTTGACGTGTATGGCCTTTTTCTTCTTTACTTGATCATCGATACTATATTTTTCTCTGTTTTGTAGCATTTGATAGTTTATGTGTTCATGTAAATTGAGAGCCAATTTCCATACAGGATTAAAGGATCAAGTTTTAGGGTTATAGTGGAAAATGAGGAACAGGTTGAGCTTTCTTTTTTGAGGATGTGGGATCCCTCCCTCGAGGGCAAGTTTGTTCCTTTGAATATCGACAAAAGGTTTGTCCTCGATTTTTTTTAATCGTTGTACTCGATCTTTCGATTGATTACTCAATTGAGCATTGCTGTTTTCTCTTCTAGGTTCATAGTGCTACGTGGTTCCTCTGGTTTCTACTCTTATGCTATTTATGAGCACTTGAAGGATTGGCCTGATTTTGATATTGGTGAAACCAGGATCACTTTTAAACTCAGAAAAGACAAGTGAGTACACTTGATCCTTTGCCTGTCATTTATGATTTCATCGAGACTCGGTATAGTGCTTCAAGCTAGGACTTGTTTATCGAGTTGCTTAATTTCTCTTAGTTGGGTTATTTTCTTTCATGTCAACATATTATTCATTAGACCAAGGATTGAATTGTTAGGTTTCAGTACATGGCTGTAGCTGACAACAGGCAGAGATTGATGCCCCTGCCCGATGATCGGTTATCTGGAAGATGCCAAACTTTGGCCTACCCGGAAGCTGTCCTGCTTTCCAATCCTAAGAACCCTCAACTCAAGGGTGAGGTACTCTTAGATTATTATTCTCTTGTTCGGGAGTGGATTGGTTGTTTGATCTTTACCTTTTGGGATGACTCAGGATTTTTAATTGTACACGTTTTCTCTTGATTCTGAAACTCAAGGTAGAGATGATTTCCAACTAGGTTTTGATAGGACCTCCCTTGCTTTGATATGATAATTTTTCGTTTAGGTGGATGACAAATACCAATACTCGTGTGAAAACAAGGACTTAAAGGTCCATGGATGGATAAGCACCAATCCATCCATCGGGTTTTGGCAGATTACACCGAGTGATGAATTTCGATCGGGTGGGCCTCATAAGCAAAGTCTAACATCCCATGTGGGACCTACAACCCTTGCTGTAGGTGATCTAACAACATTAATCGTCATTATTTTTTTGTTGTCTTGCACATTGATGTATCATAAATTCTGGAGATTTCATGTGACAATTAGGATTTCTGGTGATTCTCATTTTTCCTTTCAACTTGCTCTTTCAGATATTTCTTAGTACTCATTATGCGGGACAAGATTTGGTGCCAAAATTTAGAGCTGGTGAGCCATGGAAGAAAGTTTTTGGCCCTGTTTTTCTTTATCTTAATTCCACTTCAGCTGGAGATGATCCATTTTGGTTATGGGAGGATGCTAAAATACAGGTATGATGCATCAAGATACTCATCTTTTGTCTTTGTATGGATTACACTTTGACAAGAAATAATAATAATCATATGATTACCAGATGATGGCTGAAGTCCAAAGTTGGCCGTATAGTTTCCCTGCATCTGAGGATTACCCGAAAGTGGATCAACGGGGTACTGTTAGCGGTAGGCTTCTGGTTTTTGACAGGTAGGTAAGCATTTAATATTGTGGTAGTGTTCATATTGGTTGGAAAAATGAAATGCATTGGCCTTTGTTGGTTGCTTTACAATTCATATACTTTCAAACAGTAACAGTGAGGAACCAGTTGTTTCTGTTAACTTATTACATTTTTACGAAGAGGACATATTTGTTACATGAAAATTACGACTCCGTTCAACTTCAGGATGTAATATAGATGTTGATAAATTATCAATATCCAAAATCTATAGCAGATTTGTCCAATAATCAAAATTTGTTGTTTTTTATTCATGAATTTATTTCCTATTTTATTTTTATGGTTTCTGAAAAATTCTAAAATTAAAAATGACACTTCTGTAAATGTTGTGAAGTTAGAAATTTGGAAATGAAATTCAAGAAACCATACTCCTATATTTGTAAGACTTACAAAAAGAAAAGGAAAGGAGTTTTCCCACTTTTCAAATTTGAGATGAAAAAAGATAACAAGAAACAAAATATTATCAATTAGAACCTCGATCTGTTTAATTTTCTGGTTTAGTTTACGTGCTCGGACCTTTGTTATTAAAATGACTAGGTATATGAGTCAAGACCATCTTCCAGCCAGTGGAGCCTTTGTCGGATTGTCTGCACCAGGAGAAGTTGGGTCATGGCAGAGAGAATGCAAGGTGATTAACTTTATTTCCTCTGGTTTCAATTCTATTACAAATATGCTAGCGTAACAAAGATTATCTATTGTTGTCTACCTCGGCCAAATGAAGTTCAAACTTGTGCTTTTACAATTACTTGAGCATATCATTTTTTCCTTACTAGGATGACAGGCATACCACACCAATGAAATGATGGACCCATGTCGTATGAAGTCATTAAGAAAACTGTTCCTTCTTCACACGTGCGACAAATTTCTTCGTTGAGCTTGATATCTATTGTCTTTAAAATTCAAGATAGCAAAGCTTATCAAAAAGAGATACTTCAATTCACATGTATTTTCTTTCTAGGATTGGAATGAACAACTTTTTAAATTTCCAAATCTTTCTCATACCTATGAAAGAAGTGTTAGAGCCCTGGAAGGATAGACATCCTCTTATTTGACTTTAAGGGAAAGGATACAGAGTTCAAGGATGTTTGAGCTTTGAAGGTTTACGTCTCTCCAATGAACTAGAAAAGACTTCTAATTTCCACTGCAAGTCCCCTACCCGTTTCTTTTTCTTGTACATATAATTCCCCAGCCCTTACGTCAGTTAACTAACTTCACATTCTTTTGCTGACTTCAGTATAACTAACAACTTATAGCAAAAGACTATCCTGCCCTCCTCTCAGAGACCACCCGAACTCTCGATGATCTAACCTCCATAGTTTCACGATTCTTCCAATAAAGCCAAGAACCAGTCTCATGTTTCTATCAAGATGGTATTAAAGAAATTAGAAAAGTAATATGATGAAAGAATAAAGACAAAACTCCTTTTGCTTGAGACTGTCATCAATGTCTATGAAGTCCTTGAAAAGAAATGGAAGATAATGAATGCCTAAGCTTTTAGAACTACAATTTCTCACATGAGGTCTACTAACTCTTTGAAACTCGATCCTTTTAACTACATTTTACTATAAGAACCTTTCGTTAATAGTTTCTAGAAGGTTAAGTCACATGTGTTCTTTAAAAGAATTTATCCTCGTTGTCAGGGATATCAATTCTGGACTCGAGCAGATGATCGTGGTTATTTTACCATCGACCACATACACACCGGTGACTACAATCTATATGCATGGGTCCCTGGCTTCATTGGAGATTACCGTTGCGATGCCCTCGTCAACGTAAACCAAGGTACAGTTTCCTGTATCTGTAAATTGCCTCAAGTCCACAATTTCTCTGTACATAGTTATGGTATTCCAAATTGAAATGTAGGATCTTGCATTGAAATGGGTGATCTTGTTTATGAACCTCCAAGAGATGGGCCCACACTATGGGAAATAGGCATTCCCGATCGCTCCGCATCTGAGTTCTATGTTCCTGATCCTAACCCAATGCATATTAATAAGCTCTTTGTAAATCATCCTGACAGGTAGCCCTTTTTCTATAGTCTTATGAGTTAGATGAACTTTGATATTATGGTCCATTTTTACAATCTCTTTCACTATGTTTCTAAATAGAAATTTGAGTTCTGTTACTGAGGATTGCAGTGTGTATTAATATACCTTTGTAGAATGAAGATGATTTCTGTAGAACATGAGGGATCCAATCCAAGTCCAACTAAAGCTGAAATCTCTACCATTTAACATTTATTTATACTCTGCAGGTTCCGGCAGTATGGGTTGTGGGACAGATATGCGGAATTATACCCGGACGAGGATTTAGTATACACGGTTGGCACAAGTGATTATAGTAAAGATTGGTTCTTTGCTCAGGTCCCTAGGTAACATTTCTTTTGCTATTCATTGTCCATAACCAAGAACAACACCGACGATCATCGTCGTCATCCCCACAATTTATCAGATTAATGGCCATGATTGTTGTATGATCTTTGACATCTCCTGTATTTGTCATCAAACTATTCCATATATCTTGCAGGAAGACAAACAGTAATTCACATGAAGGAACTACTTGGCAAATCAAATTCAAACTTGACAGAGTGGATCACAAGAGTATGTTTACGCTACGAGTCGCAATCGCATCTGCAACTCTTGCTGAATTGCAGGTGAGACGCGATATCATTGGCAATAACTTAAGTGCTTAACATTTACTAAAACATGATCTCTCTCTAGGTCCGGGTTAACGATCCGAGATCGAGGCGACCTTTGTTCACGAGCGGGTTGATAGGGAGAGACAATTCAATTGCAAGACATGGAATTCATGGAATTTATTGGCTTTACAGCGTGGTCATAGCAGGCTCTCATCTTGTTGAAGGAGATAACACAATTTTCTTCACTCAGCCACGCTGCACCAGCCCTTTCCAGGGTATCATGTATGATTATATCCGTCTGGAAGGTCCTCCAACAAATCTTTGCTAA

mRNA sequence

ATGTCGTCCACGGGAGTGCGATTGCAGATACGAGATCGTCATGTGGTGGTGGATAATGGAATACTGCAACTAACTTTATCAAAACCAGATGGAATCATCACTGGCATACGATATAATGGTGTTGACAATCTGCTTGAAGTTCTCAACCCTGAATCTAACAGAGGTTATTGGGATCTCGTTTGGAATTCTCCAGGAAGCAAGGGAATATTTGACGTGATTAAAGGATCAAGTTTTAGGGTTATAGTGGAAAATGAGGAACAGGTTGAGCTTTCTTTTTTGAGGATGTGGGATCCCTCCCTCGAGGGCAAGTTTGTTCCTTTGAATATCGACAAAAGGTTCATAGTGCTACGTGGTTCCTCTGGTTTCTACTCTTATGCTATTTATGAGCACTTGAAGGATTGGCCTGATTTTGATATTGGTGAAACCAGGATCACTTTTAAACTCAGAAAAGACAAGTTTCAGTACATGGCTGTAGCTGACAACAGGCAGAGATTGATGCCCCTGCCCGATGATCGGTTATCTGGAAGATGCCAAACTTTGGCCTACCCGGAAGCTGTCCTGCTTTCCAATCCTAAGAACCCTCAACTCAAGGGTGAGGTGGATGACAAATACCAATACTCGTGTGAAAACAAGGACTTAAAGGTCCATGGATGGATAAGCACCAATCCATCCATCGGGTTTTGGCAGATTACACCGAGTGATGAATTTCGATCGGGTGGGCCTCATAAGCAAAGTCTAACATCCCATGTGGGACCTACAACCCTTGCTATATTTCTTAGTACTCATTATGCGGGACAAGATTTGGTGCCAAAATTTAGAGCTGGTGAGCCATGGAAGAAAGTTTTTGGCCCTGTTTTTCTTTATCTTAATTCCACTTCAGCTGGAGATGATCCATTTTGGTTATGGGAGGATGCTAAAATACAGATGATGGCTGAAGTCCAAAGTTGGCCGTATAGTTTCCCTGCATCTGAGGATTACCCGAAAGTGGATCAACGGGGTACTGTTAGCGGTAGGCTTCTGGTTTTTGACAGGTATATGAGTCAAGACCATCTTCCAGCCAGTGGAGCCTTTGTCGGATTGTCTGCACCAGGAGAAGTTGGGTCATGGCAGAGAGAATGCAAGGGATATCAATTCTGGACTCGAGCAGATGATCGTGGTTATTTTACCATCGACCACATACACACCGGTGACTACAATCTATATGCATGGGTCCCTGGCTTCATTGGAGATTACCGTTGCGATGCCCTCGTCAACGTAAACCAAGGATCTTGCATTGAAATGGGTGATCTTGTTTATGAACCTCCAAGAGATGGGCCCACACTATGGGAAATAGGCATTCCCGATCGCTCCGCATCTGAGTTCTATGTTCCTGATCCTAACCCAATGCATATTAATAAGCTCTTTGTAAATCATCCTGACAGGTTCCGGCAGTATGGGTTGTGGGACAGATATGCGGAATTATACCCGGACGAGGATTTAGTATACACGGTTGGCACAAGTGATTATAGTAAAGATTGGTTCTTTGCTCAGGTCCCTAGGAAGACAAACAGTAATTCACATGAAGGAACTACTTGGCAAATCAAATTCAAACTTGACAGAGTGGATCACAAGAGTATGTTTACGCTACGAGTCGCAATCGCATCTGCAACTCTTGCTGAATTGCAGGTCCGGGTTAACGATCCGAGATCGAGGCGACCTTTGTTCACGAGCGGGTTGATAGGGAGAGACAATTCAATTGCAAGACATGGAATTCATGGAATTTATTGGCTTTACAGCGTGGTCATAGCAGGCTCTCATCTTGTTGAAGGAGATAACACAATTTTCTTCACTCAGCCACGCTGCACCAGCCCTTTCCAGGGTATCATGTATGATTATATCCGTCTGGAAGGTCCTCCAACAAATCTTTGCTAA

Coding sequence (CDS)

ATGTCGTCCACGGGAGTGCGATTGCAGATACGAGATCGTCATGTGGTGGTGGATAATGGAATACTGCAACTAACTTTATCAAAACCAGATGGAATCATCACTGGCATACGATATAATGGTGTTGACAATCTGCTTGAAGTTCTCAACCCTGAATCTAACAGAGGTTATTGGGATCTCGTTTGGAATTCTCCAGGAAGCAAGGGAATATTTGACGTGATTAAAGGATCAAGTTTTAGGGTTATAGTGGAAAATGAGGAACAGGTTGAGCTTTCTTTTTTGAGGATGTGGGATCCCTCCCTCGAGGGCAAGTTTGTTCCTTTGAATATCGACAAAAGGTTCATAGTGCTACGTGGTTCCTCTGGTTTCTACTCTTATGCTATTTATGAGCACTTGAAGGATTGGCCTGATTTTGATATTGGTGAAACCAGGATCACTTTTAAACTCAGAAAAGACAAGTTTCAGTACATGGCTGTAGCTGACAACAGGCAGAGATTGATGCCCCTGCCCGATGATCGGTTATCTGGAAGATGCCAAACTTTGGCCTACCCGGAAGCTGTCCTGCTTTCCAATCCTAAGAACCCTCAACTCAAGGGTGAGGTGGATGACAAATACCAATACTCGTGTGAAAACAAGGACTTAAAGGTCCATGGATGGATAAGCACCAATCCATCCATCGGGTTTTGGCAGATTACACCGAGTGATGAATTTCGATCGGGTGGGCCTCATAAGCAAAGTCTAACATCCCATGTGGGACCTACAACCCTTGCTATATTTCTTAGTACTCATTATGCGGGACAAGATTTGGTGCCAAAATTTAGAGCTGGTGAGCCATGGAAGAAAGTTTTTGGCCCTGTTTTTCTTTATCTTAATTCCACTTCAGCTGGAGATGATCCATTTTGGTTATGGGAGGATGCTAAAATACAGATGATGGCTGAAGTCCAAAGTTGGCCGTATAGTTTCCCTGCATCTGAGGATTACCCGAAAGTGGATCAACGGGGTACTGTTAGCGGTAGGCTTCTGGTTTTTGACAGGTATATGAGTCAAGACCATCTTCCAGCCAGTGGAGCCTTTGTCGGATTGTCTGCACCAGGAGAAGTTGGGTCATGGCAGAGAGAATGCAAGGGATATCAATTCTGGACTCGAGCAGATGATCGTGGTTATTTTACCATCGACCACATACACACCGGTGACTACAATCTATATGCATGGGTCCCTGGCTTCATTGGAGATTACCGTTGCGATGCCCTCGTCAACGTAAACCAAGGATCTTGCATTGAAATGGGTGATCTTGTTTATGAACCTCCAAGAGATGGGCCCACACTATGGGAAATAGGCATTCCCGATCGCTCCGCATCTGAGTTCTATGTTCCTGATCCTAACCCAATGCATATTAATAAGCTCTTTGTAAATCATCCTGACAGGTTCCGGCAGTATGGGTTGTGGGACAGATATGCGGAATTATACCCGGACGAGGATTTAGTATACACGGTTGGCACAAGTGATTATAGTAAAGATTGGTTCTTTGCTCAGGTCCCTAGGAAGACAAACAGTAATTCACATGAAGGAACTACTTGGCAAATCAAATTCAAACTTGACAGAGTGGATCACAAGAGTATGTTTACGCTACGAGTCGCAATCGCATCTGCAACTCTTGCTGAATTGCAGGTCCGGGTTAACGATCCGAGATCGAGGCGACCTTTGTTCACGAGCGGGTTGATAGGGAGAGACAATTCAATTGCAAGACATGGAATTCATGGAATTTATTGGCTTTACAGCGTGGTCATAGCAGGCTCTCATCTTGTTGAAGGAGATAACACAATTTTCTTCACTCAGCCACGCTGCACCAGCCCTTTCCAGGGTATCATGTATGATTATATCCGTCTGGAAGGTCCTCCAACAAATCTTTGCTAA

Protein sequence

MSSTGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLVWNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSSGFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTLAYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGGPHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFWLWEDAKIQMMAEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVNQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMFTLRVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHLVEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPTNLC
Homology
BLAST of Lag0037436 vs. NCBI nr
Match: XP_038897922.1 (probable rhamnogalacturonate lyase B [Benincasa hispida])

HSP 1 Score: 1274.2 bits (3296), Expect = 0.0e+00
Identity = 589/634 (92.90%), Postives = 609/634 (96.06%), Query Frame = 0

Query: 1   MSSTGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS GVRLQIRD HVVV+NGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV
Sbjct: 1   MSSRGVRLQIRDHHVVVENGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGSKGIFDVIKGSSFRVIVENEEQVELSF+RMWDPSLEGK VPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFMRMWDPSLEGKLVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQ++MPLPDDRLSGRCQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQKVMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGG 240
           AYPEAVLL+NPKNP+LKGEVDDKYQYSCENKDLKVHGWI TNP  GFW ITPSDEFRSGG
Sbjct: 181 AYPEAVLLTNPKNPELKGEVDDKYQYSCENKDLKVHGWICTNPPTGFWHITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQSLTSHVGPTTLAIFLS HYAGQDLVPKFRAGEPWKKVFGPVF YLNSTS GDDPFW
Sbjct: 241 PLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFFYLNSTSVGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEV+SWPYSFPASED+PKVDQRG V GRLLV DRY+SQD+LPASGAFVG+
Sbjct: 301 LWEDAKIQMMAEVESWPYSFPASEDFPKVDQRGVVRGRLLVLDRYVSQDYLPASGAFVGI 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVN 420
           SAPGEVGSWQRECKGYQFWTRADD GYFTI+H HTGDYNLY+WVPGFIGDYRCDAL+NVN
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDGGYFTINHAHTGDYNLYSWVPGFIGDYRCDALINVN 420

Query: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP HINKLFVNHPDRFRQYGL
Sbjct: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPTHINKLFVNHPDRFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMF 540
           WDRYAELYP+EDLVYTVGTSDYSKDWFFAQVPRKT SNSHEGTTWQIKF L+RV  KSM+
Sbjct: 481 WDRYAELYPNEDLVYTVGTSDYSKDWFFAQVPRKTKSNSHEGTTWQIKFYLNRVARKSMY 540

Query: 541 TLRVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHL 600
           TLRVAIASATLAELQVRVNDPRS+RPLFTSGLIGRDNSIARHGIHGIYWLYSV + G HL
Sbjct: 541 TLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWLYSVGVGGCHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPTN 635
           VEG+NTIF TQPRCTSPFQGIMYDYIRLEGPPTN
Sbjct: 601 VEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPTN 634

BLAST of Lag0037436 vs. NCBI nr
Match: XP_004136250.1 (probable rhamnogalacturonate lyase B isoform X1 [Cucumis sativus] >KGN60276.1 hypothetical protein Csa_001404 [Cucumis sativus])

HSP 1 Score: 1268.1 bits (3280), Expect = 0.0e+00
Identity = 586/633 (92.58%), Postives = 609/633 (96.21%), Query Frame = 0

Query: 1   MSSTGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS  VRLQIRD HVVVDNGILQLTLSKPDGIITG+RYNGVDNLLEVLNPESNRGYWDLV
Sbjct: 1   MSSRAVRLQIRDHHVVVDNGILQLTLSKPDGIITGVRYNGVDNLLEVLNPESNRGYWDLV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGSKG+FDVIKGS FRVIVENEEQVELSF+RMWDPSLEGKFVPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSKGVFDVIKGSRFRVIVENEEQVELSFVRMWDPSLEGKFVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQR+MPLPDDRLSGRCQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRVMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGG 240
           AYPEAVLL+NPKNP LKGEVDDKYQYSCEN +LKVHGWISTNP  GFWQITPSDEFRSGG
Sbjct: 181 AYPEAVLLTNPKNPALKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTS GDDPFW
Sbjct: 241 PLKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEV SWPYSFPASED+PK DQRG VSGRLLVFDRY+ QD+LPA GAFVGL
Sbjct: 301 LWEDAKIQMMAEVDSWPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVN 420
           SAPGE GSWQRECKGYQFWTRADD GYFTI+H+HTG+YNLYAWVPGFIGDYRCDAL+NV+
Sbjct: 361 SAPGEAGSWQRECKGYQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVS 420

Query: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP+HINKLFV+HPDRFRQYGL
Sbjct: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMF 540
           W+RYAELYP+EDLVYTVGTSDY KDWFFA VPRKTN NSHEGTTWQIKFKLDRVDHKSM+
Sbjct: 481 WNRYAELYPNEDLVYTVGTSDYRKDWFFAHVPRKTN-NSHEGTTWQIKFKLDRVDHKSMY 540

Query: 541 TLRVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHL 600
           TLRVAIASATLAELQVRVNDPRS+RPLFTSGLIGRDNSIARHGIHGIYW+Y V +AG HL
Sbjct: 541 TLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPT 634
           VEG+NTIF TQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 601 VEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 632

BLAST of Lag0037436 vs. NCBI nr
Match: XP_022980958.1 (probable rhamnogalacturonate lyase B [Cucurbita maxima])

HSP 1 Score: 1262.7 bits (3266), Expect = 0.0e+00
Identity = 581/634 (91.64%), Postives = 609/634 (96.06%), Query Frame = 0

Query: 1   MSSTGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS GVRLQIRD HVVVDNGILQLTLSKPDGIITGI YNGVDNLLEVLNPESNRGYWD+V
Sbjct: 1   MSSRGVRLQIRDHHVVVDNGILQLTLSKPDGIITGIHYNGVDNLLEVLNPESNRGYWDIV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGS+GIFDVIKGSSFRVIVENEEQVELSF RMWDPSLEGK VPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSQGIFDVIKGSSFRVIVENEEQVELSFTRMWDPSLEGKVVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGG 240
            +PEAVLL+NPKNP+ + EVDDKYQYSCENKDLKVHGWIS NP IGFWQITPSDEFRSGG
Sbjct: 181 GFPEAVLLTNPKNPEHRDEVDDKYQYSCENKDLKVHGWISNNPPIGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFR GEPWKKVFGPVFLYLNST AGDDPFW
Sbjct: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRNGEPWKKVFGPVFLYLNSTKAGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEVQSWPYSFPAS+++PK+DQRGT+SGRLLVFDRYMS+D+LPASGAFVGL
Sbjct: 301 LWEDAKIQMMAEVQSWPYSFPASDNFPKLDQRGTISGRLLVFDRYMSRDYLPASGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVN 420
           SAPGEVGSWQRECKGYQFWTRADDRGYF I+H+H G YNLYAWVPGFIGDYRCDAL+N++
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFIINHVHIGQYNLYAWVPGFIGDYRCDALINIS 420

Query: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGS IE+G+LVYEPPRDGPTLWEIGIPDRSASEFY+PDPNPMHINKLFVNHP+RFRQYGL
Sbjct: 421 QGSSIELGNLVYEPPRDGPTLWEIGIPDRSASEFYIPDPNPMHINKLFVNHPERFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMF 540
           W+RYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTN+NSH+GTTW+I FKLDRVD KS +
Sbjct: 481 WERYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNNNSHQGTTWKIAFKLDRVDRKSRY 540

Query: 541 TLRVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHL 600
            LRVAIASATLAELQVRVNDP S+RPLFTSGLIGRDNSIARHGIHGIYWLYSV IAG HL
Sbjct: 541 MLRVAIASATLAELQVRVNDPSSKRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGLHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPTN 635
           VEGDN IFFTQPRCTSPFQGIMYDYIRLEGP TN
Sbjct: 601 VEGDNAIFFTQPRCTSPFQGIMYDYIRLEGPSTN 634

BLAST of Lag0037436 vs. NCBI nr
Match: XP_022940913.1 (probable rhamnogalacturonate lyase B isoform X1 [Cucurbita moschata])

HSP 1 Score: 1262.7 bits (3266), Expect = 0.0e+00
Identity = 580/634 (91.48%), Postives = 608/634 (95.90%), Query Frame = 0

Query: 1   MSSTGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS GVRLQIRD HVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWD+V
Sbjct: 1   MSSRGVRLQIRDHHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDIV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGS+GIFDVIKGSSFRVIVENEEQVELSF RMWDPSLEGK VPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSQGIFDVIKGSSFRVIVENEEQVELSFTRMWDPSLEGKVVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGG 240
            +PEAVLL+NPKNP+ + EVDDKYQYSC+NKDLKVHGWISTNP IGFWQITPSDEFRSGG
Sbjct: 181 GFPEAVLLTNPKNPEHRDEVDDKYQYSCQNKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFR GEPWKKVFGPVFLYLNST AGDDPFW
Sbjct: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRNGEPWKKVFGPVFLYLNSTKAGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEVQSWPYSFPAS+D+PK+DQRGT+SGRLLVFDRYMS+D+LPA GAFVGL
Sbjct: 301 LWEDAKIQMMAEVQSWPYSFPASDDFPKLDQRGTISGRLLVFDRYMSRDYLPAGGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVN 420
           SAPGEVGSWQRECKGYQFWTRADDRGYF I+H+H G YNLYAWVPGFIGDYRCDAL+N++
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFIINHVHIGQYNLYAWVPGFIGDYRCDALINIS 420

Query: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGS IE+GDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP+HINKLFVNHP+RFRQYGL
Sbjct: 421 QGSSIELGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPLHINKLFVNHPERFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMF 540
           W+RYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTN NSH+GTTW+I FKLDRVD K+ +
Sbjct: 481 WERYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNDNSHQGTTWKIAFKLDRVDRKNRY 540

Query: 541 TLRVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHL 600
            LRVAIASATLAELQ+RVNDP S+RPLFTSGLIGRDNSIARHGIHGIYWLYSV IAG HL
Sbjct: 541 MLRVAIASATLAELQIRVNDPSSKRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGLHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPTN 635
           VEGDN IFFTQPRCTSPFQGIMYDYIRLE P TN
Sbjct: 601 VEGDNAIFFTQPRCTSPFQGIMYDYIRLEAPSTN 634

BLAST of Lag0037436 vs. NCBI nr
Match: XP_008466137.1 (PREDICTED: probable rhamnogalacturonate lyase B isoform X1 [Cucumis melo] >KAA0038653.1 putative rhamnogalacturonate lyase B isoform X1 [Cucumis melo var. makuwa] >TYK31256.1 putative rhamnogalacturonate lyase B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1261.5 bits (3263), Expect = 0.0e+00
Identity = 586/633 (92.58%), Postives = 606/633 (95.73%), Query Frame = 0

Query: 1   MSSTGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS  VRLQIRD HVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV
Sbjct: 1   MSSKAVRLQIRDHHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGSKGIFDVIKGS F VIVENEEQVELSF+RMWDPSLEGKFVPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSKGIFDVIKGSRFSVIVENEEQVELSFVRMWDPSLEGKFVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQR+MPLPDDRLSGRCQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRVMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGG 240
           AYPEAVLL++PKN  LKGEVDDKYQYSCEN +LKVHGWISTNP  GFWQITPSDEFRSGG
Sbjct: 181 AYPEAVLLTDPKNSALKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQSLTSHVGPTTLAIFLS HYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTS GDDPFW
Sbjct: 241 PLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEV+SWPYSFPASED+ K DQRG VSGRLLVFDRY+ QD+LP SGAFVGL
Sbjct: 301 LWEDAKIQMMAEVESWPYSFPASEDFSKKDQRGMVSGRLLVFDRYVDQDYLPGSGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVN 420
           SAPGEVGSWQRECKGYQFWTRADD GYFTI+H+ TGDYNLYAWVPGFIGDYRCDALVNV+
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDGGYFTINHVLTGDYNLYAWVPGFIGDYRCDALVNVS 420

Query: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP++INKLFVNHPDRFRQYGL
Sbjct: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPIYINKLFVNHPDRFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMF 540
           WDRYAELYPDEDLVYTVGTSDY KDWFFA VPRKTNSNSHEGTTWQIKF+LDRVD KSM+
Sbjct: 481 WDRYAELYPDEDLVYTVGTSDYRKDWFFAHVPRKTNSNSHEGTTWQIKFQLDRVDRKSMY 540

Query: 541 TLRVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHL 600
            LRVAIASATLAELQVRVNDPRS+RPLFTSGLIGRDNSIARHGIHGIYW+YSV +AG HL
Sbjct: 541 MLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYSVSVAGCHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPT 634
           VEG+NTIF TQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 601 VEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 633

BLAST of Lag0037436 vs. ExPASy Swiss-Prot
Match: Q5AZ85 (Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 4.5e-22
Identity = 151/672 (22.47%), Postives = 247/672 (36.76%), Query Frame = 0

Query: 12  DRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLVWNSPGSKGIFD 71
           D  +V++N  L+ T  K  G I  +  +G D L           Y D      G      
Sbjct: 26  DTSIVLENDRLRATFDKGRGSIIDLYLDGQDFLGPQSGSTGIGPYLDCYCTPSG------ 85

Query: 72  VIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNID-KRFIVLR-GSSGFYSYAIYE 131
                S   + E  +  + +  +     L   + P   + +++  LR G +GF+ ++   
Sbjct: 86  FYTAGSTNPVTELVQGTDSTGTKYAGIILNDTYTPTGQEFQQYWFLRDGETGFHMFSRLA 145

Query: 132 HLKDWPDF--DIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTLAYPEAVL 191
           +  +   F  ++ E R  F+   D + ++  +D   +  PLP D      Q +       
Sbjct: 146 YYNETTPFLRNLQELRTLFRPNTDLWTHLTSSD--LQTAPLPSDEAIAE-QIVVQDATWR 205

Query: 192 LSNPKNP---QLKGEVDDKYQYSCENKDLKVHGW-----ISTNPSIGFWQITPSDEFRSG 251
           L+N  +    Q   E   KY +S   +D  VHG       S   + G W +  + +   G
Sbjct: 206 LNNTPDDAYYQQFSEYFTKYTFSNHWRDNDVHGLYADGSTSDGTTYGAWLVMNTKDTYYG 265

Query: 252 GPHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPF 311
           GP    LT  V        +S H+   +  P    G  + + FGP F   N   +     
Sbjct: 266 GPLHSDLT--VDGIIYNYIVSNHHG--EGTPNITNG--FDRTFGPQFYLFNGGGSSS--- 325

Query: 312 WLWEDAKIQMMAEVQSWPYSFPAS-----EDYPKVDQRGTVSGRLLVFDRYMSQDHLP-- 371
            L E           SW   F  S       Y    QRG+V G +           LP  
Sbjct: 326 -LEELRDEARSLASPSWNADFYDSIAKHVIGYVPSSQRGSVKGTI----------KLPKN 385

Query: 372 ASGAFVGLSAPGEVGSWQREC-KGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDY 431
           A      L+  G            +Q+W   D  G F+ID +  G Y L  +  G  GD+
Sbjct: 386 AKSPIAVLTVDGHYFQDNSAVPSSHQYWADIDKNGRFSIDRVVAGKYRLTVYADGIFGDF 445

Query: 432 RCDAL-VNVNQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEF-YVPDPNPMHINKLFV 491
             D + V   + + I+     ++P   G  +W +G PD+S+ EF +    +P H      
Sbjct: 446 TRDGIVVKARKSTSIKE---TWKPESAGTEIWRLGTPDKSSGEFRHGAARDPTH-----P 505

Query: 492 NHPDRFRQY-GLWDRYAELYPDEDLVYTVGTSDYSKDW------FFAQVPRKTNSNSHEG 551
            HP  +  Y G +D  ++     D  Y +G SD + D+       F   P    + S+  
Sbjct: 506 RHPPEYLIYWGAYDWQSDFPGGID--YMIGESDPATDFNTVHWAVFGPTPDNPVAESNTT 565

Query: 552 TTWQIKFKLD--RVDHKSMFTLRVAIASATLAELQVRV---NDPRSRRPLFT-------- 611
             W+I+F L   ++  +   TL + +A A  A     V   ++P +  PL +        
Sbjct: 566 HDWRIRFDLSAKQLHARKTATLTIQLAGAKAASGNTDVYNASEPYANLPLRSYINEQEEP 625

Query: 612 -SGLIGRDNS---IARHGIHGIYWLYSVVIAGSHLVEGDNTIFFTQPRCTSPFQG----- 630
            + +IG D S   I R  +             S L EG N +  + P   + ++      
Sbjct: 626 LTMVIGYDQSSSCIVRSAVSCYQVREKWEFPASWLKEGSNLLRLSLPTNGTNYESAVLPT 658

BLAST of Lag0037436 vs. ExPASy Swiss-Prot
Match: A5ABH4 (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 4.2e-20
Identity = 101/376 (26.86%), Postives = 152/376 (40.43%), Query Frame = 0

Query: 203 KYQYSCENKDLKVHGW-----ISTNPSIGFWQITPSDEFRSGGPHKQSLTSHVGPTTLAI 262
           KY +S   +D  VHG       S   + G W +  + +   GGP    LT  V       
Sbjct: 216 KYTFSNAWRDNSVHGMYADGSTSNGSTFGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNY 275

Query: 263 FLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW-LWEDAKIQMMAEVQSW 322
            +S H+   +  P    G  + + FGP + + N           L  DA  + +A+  SW
Sbjct: 276 LVSNHHG--EGTPNITYG--FDRTFGPQYYHFNGGKGSTASLQELKSDA--ETLAD-PSW 335

Query: 323 PYSFPAS-----EDYPKVDQRGTVSGRLLVFDRYMSQDHLP--ASGAFVGLSAPGEVGSW 382
              F  S       Y    QRG+V G++           LP  A+     L+  G+    
Sbjct: 336 NVDFYDSIAKHVVGYTPSSQRGSVQGKI----------KLPKGATRPIAVLTVDGQYFQD 395

Query: 383 QR-ECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVNQGSCIEMG 442
                  YQ+W   DD G+F++DH+  G Y L  +  G  GD+  D  V V  G    + 
Sbjct: 396 NSVNSSSYQYWAEIDDSGHFSVDHVKEGPYRLTVYADGIFGDFVRDG-VQVKAGKKTTIQ 455

Query: 443 DLVYEPPRDGPTLWEIGIPDRSASEF---YVPDP-NPMHINKLFVNHPDRFRQYGLWDRY 502
           +  +E    G  +W +G PD+S+ EF      DP +P+H        P+    +G +D  
Sbjct: 456 E-TWEAESAGTEIWRLGTPDKSSGEFRHGVARDPTHPLH-------PPEYLIYWGAYDWQ 515

Query: 503 AELYPDEDLVYTVGTSDYSKD-----W-FFAQVPRKTNSNSHEGTTWQIKFKL--DRVDH 553
           ++ +PD  + YT+GTSD + D     W  F   P            W I F L  D +  
Sbjct: 516 SD-FPD-GINYTIGTSDPATDLNTVHWSVFGPTPNDPRVEYDTTHDWTINFPLSEDDLAE 561

BLAST of Lag0037436 vs. ExPASy TrEMBL
Match: A0A0A0LJQ9 (Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892770 PE=3 SV=1)

HSP 1 Score: 1268.1 bits (3280), Expect = 0.0e+00
Identity = 586/633 (92.58%), Postives = 609/633 (96.21%), Query Frame = 0

Query: 1   MSSTGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS  VRLQIRD HVVVDNGILQLTLSKPDGIITG+RYNGVDNLLEVLNPESNRGYWDLV
Sbjct: 1   MSSRAVRLQIRDHHVVVDNGILQLTLSKPDGIITGVRYNGVDNLLEVLNPESNRGYWDLV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGSKG+FDVIKGS FRVIVENEEQVELSF+RMWDPSLEGKFVPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSKGVFDVIKGSRFRVIVENEEQVELSFVRMWDPSLEGKFVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQR+MPLPDDRLSGRCQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRVMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGG 240
           AYPEAVLL+NPKNP LKGEVDDKYQYSCEN +LKVHGWISTNP  GFWQITPSDEFRSGG
Sbjct: 181 AYPEAVLLTNPKNPALKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTS GDDPFW
Sbjct: 241 PLKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEV SWPYSFPASED+PK DQRG VSGRLLVFDRY+ QD+LPA GAFVGL
Sbjct: 301 LWEDAKIQMMAEVDSWPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVN 420
           SAPGE GSWQRECKGYQFWTRADD GYFTI+H+HTG+YNLYAWVPGFIGDYRCDAL+NV+
Sbjct: 361 SAPGEAGSWQRECKGYQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVS 420

Query: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP+HINKLFV+HPDRFRQYGL
Sbjct: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMF 540
           W+RYAELYP+EDLVYTVGTSDY KDWFFA VPRKTN NSHEGTTWQIKFKLDRVDHKSM+
Sbjct: 481 WNRYAELYPNEDLVYTVGTSDYRKDWFFAHVPRKTN-NSHEGTTWQIKFKLDRVDHKSMY 540

Query: 541 TLRVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHL 600
           TLRVAIASATLAELQVRVNDPRS+RPLFTSGLIGRDNSIARHGIHGIYW+Y V +AG HL
Sbjct: 541 TLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPT 634
           VEG+NTIF TQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 601 VEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 632

BLAST of Lag0037436 vs. ExPASy TrEMBL
Match: A0A6J1J0S6 (Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111480252 PE=3 SV=1)

HSP 1 Score: 1262.7 bits (3266), Expect = 0.0e+00
Identity = 581/634 (91.64%), Postives = 609/634 (96.06%), Query Frame = 0

Query: 1   MSSTGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS GVRLQIRD HVVVDNGILQLTLSKPDGIITGI YNGVDNLLEVLNPESNRGYWD+V
Sbjct: 1   MSSRGVRLQIRDHHVVVDNGILQLTLSKPDGIITGIHYNGVDNLLEVLNPESNRGYWDIV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGS+GIFDVIKGSSFRVIVENEEQVELSF RMWDPSLEGK VPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSQGIFDVIKGSSFRVIVENEEQVELSFTRMWDPSLEGKVVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGG 240
            +PEAVLL+NPKNP+ + EVDDKYQYSCENKDLKVHGWIS NP IGFWQITPSDEFRSGG
Sbjct: 181 GFPEAVLLTNPKNPEHRDEVDDKYQYSCENKDLKVHGWISNNPPIGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFR GEPWKKVFGPVFLYLNST AGDDPFW
Sbjct: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRNGEPWKKVFGPVFLYLNSTKAGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEVQSWPYSFPAS+++PK+DQRGT+SGRLLVFDRYMS+D+LPASGAFVGL
Sbjct: 301 LWEDAKIQMMAEVQSWPYSFPASDNFPKLDQRGTISGRLLVFDRYMSRDYLPASGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVN 420
           SAPGEVGSWQRECKGYQFWTRADDRGYF I+H+H G YNLYAWVPGFIGDYRCDAL+N++
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFIINHVHIGQYNLYAWVPGFIGDYRCDALINIS 420

Query: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGS IE+G+LVYEPPRDGPTLWEIGIPDRSASEFY+PDPNPMHINKLFVNHP+RFRQYGL
Sbjct: 421 QGSSIELGNLVYEPPRDGPTLWEIGIPDRSASEFYIPDPNPMHINKLFVNHPERFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMF 540
           W+RYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTN+NSH+GTTW+I FKLDRVD KS +
Sbjct: 481 WERYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNNNSHQGTTWKIAFKLDRVDRKSRY 540

Query: 541 TLRVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHL 600
            LRVAIASATLAELQVRVNDP S+RPLFTSGLIGRDNSIARHGIHGIYWLYSV IAG HL
Sbjct: 541 MLRVAIASATLAELQVRVNDPSSKRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGLHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPTN 635
           VEGDN IFFTQPRCTSPFQGIMYDYIRLEGP TN
Sbjct: 601 VEGDNAIFFTQPRCTSPFQGIMYDYIRLEGPSTN 634

BLAST of Lag0037436 vs. ExPASy TrEMBL
Match: A0A6J1FQM1 (Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111446358 PE=3 SV=1)

HSP 1 Score: 1262.7 bits (3266), Expect = 0.0e+00
Identity = 580/634 (91.48%), Postives = 608/634 (95.90%), Query Frame = 0

Query: 1   MSSTGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS GVRLQIRD HVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWD+V
Sbjct: 1   MSSRGVRLQIRDHHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDIV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGS+GIFDVIKGSSFRVIVENEEQVELSF RMWDPSLEGK VPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSQGIFDVIKGSSFRVIVENEEQVELSFTRMWDPSLEGKVVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGG 240
            +PEAVLL+NPKNP+ + EVDDKYQYSC+NKDLKVHGWISTNP IGFWQITPSDEFRSGG
Sbjct: 181 GFPEAVLLTNPKNPEHRDEVDDKYQYSCQNKDLKVHGWISTNPPIGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFR GEPWKKVFGPVFLYLNST AGDDPFW
Sbjct: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRNGEPWKKVFGPVFLYLNSTKAGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEVQSWPYSFPAS+D+PK+DQRGT+SGRLLVFDRYMS+D+LPA GAFVGL
Sbjct: 301 LWEDAKIQMMAEVQSWPYSFPASDDFPKLDQRGTISGRLLVFDRYMSRDYLPAGGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVN 420
           SAPGEVGSWQRECKGYQFWTRADDRGYF I+H+H G YNLYAWVPGFIGDYRCDAL+N++
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFIINHVHIGQYNLYAWVPGFIGDYRCDALINIS 420

Query: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGS IE+GDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP+HINKLFVNHP+RFRQYGL
Sbjct: 421 QGSSIELGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPLHINKLFVNHPERFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMF 540
           W+RYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTN NSH+GTTW+I FKLDRVD K+ +
Sbjct: 481 WERYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNDNSHQGTTWKIAFKLDRVDRKNRY 540

Query: 541 TLRVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHL 600
            LRVAIASATLAELQ+RVNDP S+RPLFTSGLIGRDNSIARHGIHGIYWLYSV IAG HL
Sbjct: 541 MLRVAIASATLAELQIRVNDPSSKRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGLHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPTN 635
           VEGDN IFFTQPRCTSPFQGIMYDYIRLE P TN
Sbjct: 601 VEGDNAIFFTQPRCTSPFQGIMYDYIRLEAPSTN 634

BLAST of Lag0037436 vs. ExPASy TrEMBL
Match: A0A5D3E6J1 (Rhamnogalacturonan endolyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005450 PE=3 SV=1)

HSP 1 Score: 1261.5 bits (3263), Expect = 0.0e+00
Identity = 586/633 (92.58%), Postives = 606/633 (95.73%), Query Frame = 0

Query: 1   MSSTGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS  VRLQIRD HVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV
Sbjct: 1   MSSKAVRLQIRDHHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGSKGIFDVIKGS F VIVENEEQVELSF+RMWDPSLEGKFVPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSKGIFDVIKGSRFSVIVENEEQVELSFVRMWDPSLEGKFVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQR+MPLPDDRLSGRCQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRVMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGG 240
           AYPEAVLL++PKN  LKGEVDDKYQYSCEN +LKVHGWISTNP  GFWQITPSDEFRSGG
Sbjct: 181 AYPEAVLLTDPKNSALKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQSLTSHVGPTTLAIFLS HYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTS GDDPFW
Sbjct: 241 PLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEV+SWPYSFPASED+ K DQRG VSGRLLVFDRY+ QD+LP SGAFVGL
Sbjct: 301 LWEDAKIQMMAEVESWPYSFPASEDFSKKDQRGMVSGRLLVFDRYVDQDYLPGSGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVN 420
           SAPGEVGSWQRECKGYQFWTRADD GYFTI+H+ TGDYNLYAWVPGFIGDYRCDALVNV+
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDGGYFTINHVLTGDYNLYAWVPGFIGDYRCDALVNVS 420

Query: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP++INKLFVNHPDRFRQYGL
Sbjct: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPIYINKLFVNHPDRFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMF 540
           WDRYAELYPDEDLVYTVGTSDY KDWFFA VPRKTNSNSHEGTTWQIKF+LDRVD KSM+
Sbjct: 481 WDRYAELYPDEDLVYTVGTSDYRKDWFFAHVPRKTNSNSHEGTTWQIKFQLDRVDRKSMY 540

Query: 541 TLRVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHL 600
            LRVAIASATLAELQVRVNDPRS+RPLFTSGLIGRDNSIARHGIHGIYW+YSV +AG HL
Sbjct: 541 MLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYSVSVAGCHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPT 634
           VEG+NTIF TQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 601 VEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 633

BLAST of Lag0037436 vs. ExPASy TrEMBL
Match: A0A1S3CQJ4 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503644 PE=3 SV=1)

HSP 1 Score: 1261.5 bits (3263), Expect = 0.0e+00
Identity = 586/633 (92.58%), Postives = 606/633 (95.73%), Query Frame = 0

Query: 1   MSSTGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS  VRLQIRD HVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV
Sbjct: 1   MSSKAVRLQIRDHHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WNSPGSKGIFDVIKGS F VIVENEEQVELSF+RMWDPSLEGKFVPLNIDKRFIVLRGSS
Sbjct: 61  WNSPGSKGIFDVIKGSRFSVIVENEEQVELSFVRMWDPSLEGKFVPLNIDKRFIVLRGSS 120

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180
           GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQR+MPLPDDRLSGRCQTL
Sbjct: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRVMPLPDDRLSGRCQTL 180

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGG 240
           AYPEAVLL++PKN  LKGEVDDKYQYSCEN +LKVHGWISTNP  GFWQITPSDEFRSGG
Sbjct: 181 AYPEAVLLTDPKNSALKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGG 240

Query: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQSLTSHVGPTTLAIFLS HYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTS GDDPFW
Sbjct: 241 PLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFW 300

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LWEDAKIQMMAEV+SWPYSFPASED+ K DQRG VSGRLLVFDRY+ QD+LP SGAFVGL
Sbjct: 301 LWEDAKIQMMAEVESWPYSFPASEDFSKKDQRGMVSGRLLVFDRYVDQDYLPGSGAFVGL 360

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVN 420
           SAPGEVGSWQRECKGYQFWTRADD GYFTI+H+ TGDYNLYAWVPGFIGDYRCDALVNV+
Sbjct: 361 SAPGEVGSWQRECKGYQFWTRADDGGYFTINHVLTGDYNLYAWVPGFIGDYRCDALVNVS 420

Query: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
           QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNP++INKLFVNHPDRFRQYGL
Sbjct: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPIYINKLFVNHPDRFRQYGL 480

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMF 540
           WDRYAELYPDEDLVYTVGTSDY KDWFFA VPRKTNSNSHEGTTWQIKF+LDRVD KSM+
Sbjct: 481 WDRYAELYPDEDLVYTVGTSDYRKDWFFAHVPRKTNSNSHEGTTWQIKFQLDRVDRKSMY 540

Query: 541 TLRVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHL 600
            LRVAIASATLAELQVRVNDPRS+RPLFTSGLIGRDNSIARHGIHGIYW+YSV +AG HL
Sbjct: 541 MLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYSVSVAGCHL 600

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPT 634
           VEG+NTIF TQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 601 VEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 633

BLAST of Lag0037436 vs. TAIR 10
Match: AT1G09910.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 1010.4 bits (2611), Expect = 6.5e-295
Identity = 452/633 (71.41%), Postives = 534/633 (84.36%), Query Frame = 0

Query: 1   MSSTGVRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLV 60
           MSS GV L + DR+VV+DNGILQ+TLSKP GIITGI YNG+DN+LEV N E+NRGYWDL 
Sbjct: 44  MSSHGVHLHVHDRYVVMDNGILQVTLSKPGGIITGIEYNGIDNVLEVRNKETNRGYWDLH 103

Query: 61  WNSPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSS 120
           WN PG KGIFDVI G +FRVIVE EEQVE+SFLR WDPSLEGK++PLNIDKRFI+LRGSS
Sbjct: 104 WNEPGGKGIFDVISGVTFRVIVETEEQVEISFLRTWDPSLEGKYIPLNIDKRFIMLRGSS 163

Query: 121 GFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTL 180
           G YSY IYEHLKDWP F++GETRI FKLRKDKF YMAVAD+R+R+MP PDD   GRCQTL
Sbjct: 164 GVYSYGIYEHLKDWPGFELGETRIAFKLRKDKFHYMAVADDRKRIMPFPDDLCKGRCQTL 223

Query: 181 AYPEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGG 240
            Y EA LL+ P +P+L+GEVDDKYQYSCENKDL+VHGWIS +P +GFWQITPS+EFRSGG
Sbjct: 224 DYQEASLLTAPCDPRLQGEVDDKYQYSCENKDLRVHGWISFDPPVGFWQITPSNEFRSGG 283

Query: 241 PHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFW 300
           P KQ+LTSHVGPTTLA+F STHYAG+ ++P+F  GEPWKKV+GPVF+YLNST+ GDDP  
Sbjct: 284 PLKQNLTSHVGPTTLAVFHSTHYAGKTMMPRFEHGEPWKKVYGPVFIYLNSTANGDDPLC 343

Query: 301 LWEDAKIQMMAEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGL 360
           LW+DAKI+MMAEV+ WPYSF AS+DYPK ++RGT  GRLL+ DR+++ D + A GA+VGL
Sbjct: 344 LWDDAKIKMMAEVERWPYSFVASDDYPKSEERGTARGRLLIRDRFINNDLISARGAYVGL 403

Query: 361 SAPGEVGSWQRECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVN 420
           + PG+ GSWQ ECKGYQFW  AD+ GYF+I ++  G+YNLYAWVP FIGDY    +V V 
Sbjct: 404 APPGDSGSWQIECKGYQFWAIADEAGYFSIGNVRPGEYNLYAWVPSFIGDYHNGTIVRVT 463

Query: 421 QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGL 480
            G  IEMGD+VYEPPRDGPTLWEIGIPDR ASEF++PDP+P  +N++ V+H DRFRQYGL
Sbjct: 464 SGCMIEMGDIVYEPPRDGPTLWEIGIPDRKASEFFIPDPDPTLVNRVLVHHQDRFRQYGL 523

Query: 481 WDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMF 540
           W +Y ++YP++DLVYTVG SDY +DWFFA VPRK   + HEGTTWQI F L+ +D K+ +
Sbjct: 524 WKKYTDMYPNDDLVYTVGVSDYRRDWFFAHVPRK-KGDVHEGTTWQIIFNLENIDQKANY 583

Query: 541 TLRVAIASATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHL 600
            LRVAIASATLAELQ+R+ND  + RPLFT+GLIGRDNSIARHGIHG+Y LY+V I G+ L
Sbjct: 584 KLRVAIASATLAELQIRINDAEAIRPLFTTGLIGRDNSIARHGIHGVYMLYAVNIPGNRL 643

Query: 601 VEGDNTIFFTQPRCTSPFQGIMYDYIRLEGPPT 634
           V+GDNTIF  QPRC  PFQGIMYDYIRLEGPP+
Sbjct: 644 VQGDNTIFLKQPRCNGPFQGIMYDYIRLEGPPS 675

BLAST of Lag0037436 vs. TAIR 10
Match: AT1G09890.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 960.3 bits (2481), Expect = 7.7e-280
Identity = 433/617 (70.18%), Postives = 507/617 (82.17%), Query Frame = 0

Query: 17  VDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLVWNSPGSKGIFDVIKGS 76
           +DNGI ++TLSKPDGI+TGI YNG+DNLLEVLN E NRGYWDLVW   G+ G FDVIKGS
Sbjct: 1   MDNGIARVTLSKPDGIVTGIEYNGIDNLLEVLNEEVNRGYWDLVWGGSGTAGGFDVIKGS 60

Query: 77  SFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSSGFYSYAIYEHLKDWPD 136
           +F VIV+NEEQ+ELSF R WDPS EGK VPLNIDKRF++L GSSGFY+YAIYEHLK+WP 
Sbjct: 61  NFEVIVKNEEQIELSFTRKWDPSQEGKAVPLNIDKRFVMLSGSSGFYTYAIYEHLKEWPA 120

Query: 137 FDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTLAYPEAVLLSNPKNPQL 196
           F + ETRI FKLRK+KF YMAV D+RQR MPLPDDRL  R Q LAYPEAVLL NP   Q 
Sbjct: 121 FSLAETRIAFKLRKEKFHYMAVTDDRQRFMPLPDDRLPDRGQALAYPEAVLLVNPLESQF 180

Query: 197 KGEVDDKYQYSCENKDLKVHGWIST-NPSIGFWQITPSDEFRSGGPHKQSLTSHVGPTTL 256
           KGEVDDKYQYSCENKD+ VHGWI T  PS+GFW ITPS E+R+GGP KQ+LTSHVGPT L
Sbjct: 181 KGEVDDKYQYSCENKDITVHGWICTEQPSVGFWLITPSHEYRTGGPQKQNLTSHVGPTAL 240

Query: 257 AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGD-DPFWLWEDAKIQMMAEVQ 316
           A+F+S HY G+DLVPKF  GE WKKVFGPVF+YLNS++  D DP WLW+DAK QM  E +
Sbjct: 241 AVFISAHYTGEDLVPKFSEGEAWKKVFGPVFVYLNSSTDDDNDPLWLWQDAKSQMNVEAE 300

Query: 317 SWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGLSAPGEVGSWQRECK 376
           SWPYSFPAS+DY K +QRG V GRLLV DRY+ +D + A+  +VGL+ PG  GSWQRECK
Sbjct: 301 SWPYSFPASDDYVKTEQRGNVVGRLLVQDRYVDKDFIAANRGYVGLAVPGAAGSWQRECK 360

Query: 377 GYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVNQGSCIEMGDLVYEP 436
            YQFWTR D+ G+F I  I  G YNLYAW+PGFIGDY+ D ++ +  G  I + DLVY+P
Sbjct: 361 EYQFWTRTDEEGFFYISGIRPGQYNLYAWIPGFIGDYKYDDVITITSGCYIYVEDLVYQP 420

Query: 437 PRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGLWDRYAELYPDEDLV 496
           PR+G TLWEIG PDRSA+EFYVPDPNP +IN L+ NHPDRFRQYGLW+RYAELYPD+DLV
Sbjct: 421 PRNGATLWEIGFPDRSAAEFYVPDPNPKYINNLYQNHPDRFRQYGLWERYAELYPDKDLV 480

Query: 497 YTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMFTLRVAIASATLAEL 556
           Y VG+SDY KDWF+AQV RK ++ +++GTTWQIKF+L  +D    +TLRVAIASAT +EL
Sbjct: 481 YVVGSSDYRKDWFYAQVTRKKDNKTYQGTTWQIKFELKNIDKNHSYTLRVAIASATFSEL 540

Query: 557 QVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHLVEGDNTIFFTQPRC 616
           Q+RVN+  +  P+FTSGLIGRDNSIARHGIHG+YWL++V +AGS L+EG+NT+F TQPR 
Sbjct: 541 QIRVNNANA-SPMFTSGLIGRDNSIARHGIHGLYWLFNVEVAGSKLLEGENTLFLTQPRS 600

Query: 617 TSPFQGIMYDYIRLEGP 632
           TSPFQGIMYDYIR E P
Sbjct: 601 TSPFQGIMYDYIRFEAP 616

BLAST of Lag0037436 vs. TAIR 10
Match: AT4G24430.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 878.2 bits (2268), Expect = 3.9e-255
Identity = 400/634 (63.09%), Postives = 500/634 (78.86%), Query Frame = 0

Query: 6   VRLQIRDRHVVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLVWN--- 65
           V+L +++ HVV+ NG +++T+SKPDG +TGI Y GVDNLLE  N + NRGYWDLVW+   
Sbjct: 7   VQLDVQESHVVMGNGKVKVTISKPDGFVTGISYQGVDNLLETHNEDFNRGYWDLVWSDEG 66

Query: 66  SPGSKGIFDVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSSGF 125
           +PG+ G  + IKG+SF V+VENEE VE+SF R WD SL+    P+N+DKRFI+ +  +GF
Sbjct: 67  TPGTTGKSERIKGTSFEVVVENEELVEISFSRKWDSSLQDSIAPINVDKRFIMRKDVTGF 126

Query: 126 YSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTLAY 185
           YSYAI+EHL +WP F++ +TRI +KLRKDKF+YMA+ADNRQR MPLP+DRL  R + LAY
Sbjct: 127 YSYAIFEHLAEWPAFNLPQTRIVYKLRKDKFKYMAIADNRQRKMPLPEDRLGKRGRPLAY 186

Query: 186 PEAVLLSNPKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGGPH 245
           PEAVLL +P   + KGEVDDKY+YS ENKDLKVHGWIS N  +G WQI PS+EFRSGG  
Sbjct: 187 PEAVLLVHPVEDEFKGEVDDKYEYSSENKDLKVHGWISHNLDLGCWQIIPSNEFRSGGLS 246

Query: 246 KQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNS-TSAGDDPFWL 305
           KQ+LTSHVGP +LA+FLS HYAG+D+V K +AG+ WKKVFGPVF YLN       DP  L
Sbjct: 247 KQNLTSHVGPISLAMFLSAHYAGEDMVMKVKAGDSWKKVFGPVFTYLNCLPDKTSDPLSL 306

Query: 306 WEDAKIQMMAEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGLS 365
           W+DAK QM+ EVQSWPY FPASED+P  D+RG +SGRLLV D+++S D LPA+GAFVGL+
Sbjct: 307 WQDAKNQMLTEVQSWPYDFPASEDFPVSDKRGCISGRLLVCDKFLSDDFLPANGAFVGLA 366

Query: 366 APGEVGSWQRECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVNQ 425
            PGEVGSWQ E KGYQFWT AD  GYF I+ I  G+YNL  +V G+IGDY+ + L+N+  
Sbjct: 367 PPGEVGSWQLESKGYQFWTEADSDGYFAINDIREGEYNLNGYVTGWIGDYQYEQLINITA 426

Query: 426 GSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGLW 485
           G  I++G++VYEPPRDGPT+WEIGIPDRSA+EF+VPDPNP +INKL++ HPDRFRQYGLW
Sbjct: 427 GCDIDVGNIVYEPPRDGPTVWEIGIPDRSAAEFFVPDPNPKYINKLYIGHPDRFRQYGLW 486

Query: 486 DRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMFT 545
           +RY ELYP EDLV+T+G SDY KDWFFA V RK   ++++ TTWQIKFKL+ V     + 
Sbjct: 487 ERYTELYPKEDLVFTIGVSDYKKDWFFAHVTRKMGDDTYQKTTWQIKFKLENVQKSCTYK 546

Query: 546 LRVAIASATLAELQVRVNDPRSRR--PLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSH 605
           +R+A+A+A +AELQVR+ND  + +  P+FT+G+IG DN+IARHGIHGIY LY+V +    
Sbjct: 547 IRIALATANVAELQVRMNDDDTEKTTPIFTTGVIGHDNAIARHGIHGIYRLYNVDVPSEK 606

Query: 606 LVEGDNTIFFTQPRCTS-PFQGIMYDYIRLEGPP 633
           LVEGDNT+F TQ   T+  F G+MYDYIRLEGPP
Sbjct: 607 LVEGDNTLFLTQTMTTTGAFNGLMYDYIRLEGPP 640

BLAST of Lag0037436 vs. TAIR 10
Match: AT1G09880.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 833.9 bits (2153), Expect = 8.4e-242
Identity = 378/623 (60.67%), Postives = 481/623 (77.21%), Query Frame = 0

Query: 17  VDNGILQLTLSKPDGIITGIRYNGVDNLLE-VLNPESNRGYWDLVWNSPG-----SKGIF 76
           ++N  LQLTLS P+G +TGI+YNG+DN+L    N E +RGYWD+VWN PG     +KG  
Sbjct: 1   MENRFLQLTLSNPEGFVTGIQYNGIDNVLAYYTNKEYDRGYWDVVWNFPGKKAKKTKGTL 60

Query: 77  DVIKGSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSSGFYSYAIYEH 136
           D I+ +   VI +N+E++ELSF R W+ S     VP+NIDKRF++L+ SSGFYSYAI+E 
Sbjct: 61  DRIEATKMEVITQNDEKIELSFTRTWNTS-STTAVPVNIDKRFVMLQNSSGFYSYAIFER 120

Query: 137 LKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTLAYPEAVLLSN 196
           L+ WP  ++   R+ FKL K KF YMA++D+RQR MP+PDDR+  R Q LAYPEAV L +
Sbjct: 121 LQGWPAVELDNMRLVFKLNKKKFHYMAISDDRQRYMPVPDDRVPPRGQPLAYPEAVQLLD 180

Query: 197 PKNPQLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGGPHKQSLTSHV 256
           P  P+ KGEVDDKY+YS E+KD+KVHGWISTN S+GFWQITPS+EFRS GP KQ L SHV
Sbjct: 181 PIEPEFKGEVDDKYEYSMESKDIKVHGWISTNDSVGFWQITPSNEFRSAGPLKQFLGSHV 240

Query: 257 GPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFWLWEDAKIQMM 316
           GPT LA+F STHY G DL+  F+ GE WKKVFGPVF+YLNS   G DP  LW +AK Q  
Sbjct: 241 GPTNLAVFHSTHYVGADLIMSFKNGEAWKKVFGPVFIYLNSFPKGVDPLLLWHEAKNQTK 300

Query: 317 AEVQSWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGLSAPGEVGSWQ 376
            E + WPY+F AS+D+P  DQRG+VSGRLLV DR++S + +PA+G++VGL+APG+VGSWQ
Sbjct: 301 IEEEKWPYNFTASDDFPASDQRGSVSGRLLVRDRFISSEDIPANGSYVGLAAPGDVGSWQ 360

Query: 377 RECKGYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVNQGSCIEMGDL 436
           RECKGYQFW++AD+ G F+I+++ +G YNLYA+ PGFIGDY  D + +++ GS I +GDL
Sbjct: 361 RECKGYQFWSKADENGSFSINNVRSGRYNLYAFAPGFIGDYHNDTVFDISPGSKISLGDL 420

Query: 437 VYEPPRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNHPDRFRQYGLWDRYAELYPD 496
           VYEPPRDG TLWEIG+PDRSA+EFY+PDPNP  +NKL++NH D++RQYGLW+RY+ELYPD
Sbjct: 421 VYEPPRDGSTLWEIGVPDRSAAEFYIPDPNPSFVNKLYLNHSDKYRQYGLWERYSELYPD 480

Query: 497 EDLVYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLD--RVDHKSMFTLRVAIAS 556
           ED+VY V   DYSK+WFF QV RK  +  ++GTTWQI+F+ D    +    F LR+A+A+
Sbjct: 481 EDMVYNVDIDDYSKNWFFMQVTRKQANGGYKGTTWQIRFQFDDKMKNVTGNFKLRIALAT 540

Query: 557 ATLAELQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHLVEGDNTIF 616
           + +AELQVRVND  +  PLF +  IGRDN+IARHGIHG+YWLYSV +  + L  G+NTI+
Sbjct: 541 SNVAELQVRVNDLSADPPLFRTEQIGRDNTIARHGIHGLYWLYSVNVPAASLHVGNNTIY 600

Query: 617 FTQPRCTSPFQGIMYDYIRLEGP 632
            TQ   TSPFQG+MYDYIRLE P
Sbjct: 601 LTQALATSPFQGLMYDYIRLEYP 622

BLAST of Lag0037436 vs. TAIR 10
Match: AT2G22620.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 788.1 bits (2034), Expect = 5.3e-228
Identity = 363/618 (58.74%), Postives = 456/618 (73.79%), Query Frame = 0

Query: 15  VVVDNGILQLTLSKPDGIITGIRYNGVDNLLEVLNPESNRGYWDLVWNSPGSKGIFDVIK 74
           VVVDNGI+Q+T S P+G+ITGI+Y+G+DN+L+  +   +RGYWD+VW  P  K   D ++
Sbjct: 62  VVVDNGIVQVTFSNPEGLITGIKYHGIDNVLD--DKIDDRGYWDVVWYEPEKKQKTDKLE 121

Query: 75  GSSFRVIVENEEQVELSFLRMWDPSLEGKFVPLNIDKRFIVLRGSSGFYSYAIYEHLKDW 134
           G+ F +I +NEEQ+E+SF R W  S  G  VPLN+DKR+I+  G SG Y Y I E L+ W
Sbjct: 122 GTKFEIITQNEEQIEISFTRTWTISRRGSLVPLNVDKRYIIRSGVSGLYMYGILERLEGW 181

Query: 135 PDFDIGETRITFKLRKDKFQYMAVADNRQRLMPLPDDRLSGRCQTLAYPEAVLLSNPKNP 194
           PD D+ + RI FKL   KF +MA++D+RQR MP   DR + +  +LAY EAVLL+NP NP
Sbjct: 182 PDVDMDQIRIVFKLNPKKFDFMAISDDRQRSMPSMADRENSK--SLAYKEAVLLTNPSNP 241

Query: 195 QLKGEVDDKYQYSCENKDLKVHGWISTNPSIGFWQITPSDEFRSGGPHKQSLTSHVGPTT 254
             KGEVDDKY YS E+KD  VHGWIS++P +GFW ITPSDEFR GGP KQ LTSH GP T
Sbjct: 242 MFKGEVDDKYMYSMEDKDNNVHGWISSDPPVGFWMITPSDEFRLGGPIKQDLTSHAGPIT 301

Query: 255 LAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSAGDDPFWLWEDAKIQMMAEVQ 314
           L++F STHYAG+++   +R GEPWKKVFGPV  YLNS S  D    LW DAK QM AEV+
Sbjct: 302 LSMFTSTHYAGKEMRMDYRNGEPWKKVFGPVLAYLNSVSPKDSTLRLWRDAKRQMAAEVK 361

Query: 315 SWPYSFPASEDYPKVDQRGTVSGRLLVFDRYMSQDHLPASGAFVGLSAPGEVGSWQRECK 374
           SWPY F  SEDYP   QRGT+ G+ L+ D Y+S+  +    AFVGL+  GE GSWQ E K
Sbjct: 362 SWPYDFITSEDYPLRHQRGTLEGQFLIKDSYVSRLKIYGKFAFVGLAPIGEAGSWQTESK 421

Query: 375 GYQFWTRADDRGYFTIDHIHTGDYNLYAWVPGFIGDYRCDALVNVNQGSCIEMGDLVYEP 434
           GYQFWT+AD RG F I+++  G+Y+LYAW  GFIGDY+ +  + +  GS + +G LVYEP
Sbjct: 422 GYQFWTKADRRGRFIIENVRAGNYSLYAWGSGFIGDYKYEQNITITPGSEMNVGPLVYEP 481

Query: 435 PRDGPTLWEIGIPDRSASEFYVPDPNPMHINKLFVNH-PDRFRQYGLWDRYAELYPDEDL 494
           PR+GPTLWEIG+PDR+A EFY+PDP P  +NKL+VN   DRFRQYGLWDRYA+LYP  DL
Sbjct: 482 PRNGPTLWEIGVPDRTAGEFYIPDPYPTLMNKLYVNPLQDRFRQYGLWDRYADLYPQNDL 541

Query: 495 VYTVGTSDYSKDWFFAQVPRKTNSNSHEGTTWQIKFKLDRVDHKSMFTLRVAIASATLAE 554
           VYT+G SDY  DWFFA V R   +++++ TTWQI F L  V+    +TLR+A+ASA  +E
Sbjct: 542 VYTIGVSDYRSDWFFAHVARNVGNDTYQPTTWQIIFNLKNVNRIGRYTLRIALASAADSE 601

Query: 555 LQVRVNDPRSRRPLFTSGLIGRDNSIARHGIHGIYWLYSVVIAGSHLVEGDNTIFFTQPR 614
           LQ+R+NDP+S   +FT+G IG+DN+IARHGIHG+Y LYS+ +AG+ L  GDNTIF TQ R
Sbjct: 602 LQIRINDPKS-DAIFTTGFIGKDNAIARHGIHGLYRLYSIDVAGNLLSVGDNTIFLTQTR 661

Query: 615 CTSPFQGIMYDYIRLEGP 632
             +PFQGIMYDYIRLE P
Sbjct: 662 SRTPFQGIMYDYIRLESP 674

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897922.10.0e+0092.90probable rhamnogalacturonate lyase B [Benincasa hispida][more]
XP_004136250.10.0e+0092.58probable rhamnogalacturonate lyase B isoform X1 [Cucumis sativus] >KGN60276.1 hy... [more]
XP_022980958.10.0e+0091.64probable rhamnogalacturonate lyase B [Cucurbita maxima][more]
XP_022940913.10.0e+0091.48probable rhamnogalacturonate lyase B isoform X1 [Cucurbita moschata][more]
XP_008466137.10.0e+0092.58PREDICTED: probable rhamnogalacturonate lyase B isoform X1 [Cucumis melo] >KAA00... [more]
Match NameE-valueIdentityDescription
Q5AZ854.5e-2222.47Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 ... [more]
A5ABH44.2e-2026.86Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / F... [more]
Match NameE-valueIdentityDescription
A0A0A0LJQ90.0e+0092.58Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892770 PE=3 SV=... [more]
A0A6J1J0S60.0e+0091.64Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111480252 PE=3 SV... [more]
A0A6J1FQM10.0e+0091.48Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111446358 PE=3 ... [more]
A0A5D3E6J10.0e+0092.58Rhamnogalacturonan endolyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CQJ40.0e+0092.58Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503644 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G09910.16.5e-29571.41Rhamnogalacturonate lyase family protein [more]
AT1G09890.17.7e-28070.18Rhamnogalacturonate lyase family protein [more]
AT4G24430.13.9e-25563.09Rhamnogalacturonate lyase family protein [more]
AT1G09880.18.4e-24260.67Rhamnogalacturonate lyase family protein [more]
AT2G22620.15.3e-22858.74Rhamnogalacturonate lyase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029411Rhamnogalacturonan lyase, domain IIIPFAMPF14683CBM-likecoord: 440..628
e-value: 1.9E-56
score: 190.6
IPR010325Rhamnogalacturonate lyasePFAMPF06045Rhamnogal_lyasecoord: 9..200
e-value: 8.1E-92
score: 306.5
NoneNo IPR availableGENE3D2.60.120.260coord: 438..629
e-value: 6.1E-21
score: 76.7
NoneNo IPR availableGENE3D2.60.40.1120coord: 329..425
e-value: 4.0E-6
score: 29.0
NoneNo IPR availablePANTHERPTHR32018RHAMNOGALACTURONATE LYASE FAMILY PROTEINcoord: 1..633
NoneNo IPR availablePANTHERPTHR32018:SF8RHAMNOGALACTURONATE LYASE FAMILY PROTEINcoord: 1..633
NoneNo IPR availableCDDcd10320RGL4_Ncoord: 6..297
e-value: 6.03085E-81
score: 254.621
NoneNo IPR availableCDDcd10317RGL4_Ccoord: 442..629
e-value: 1.15977E-56
score: 187.098
IPR029413Rhamnogalacturonan lyase, domain IIPFAMPF14686fn3_3coord: 354..425
e-value: 1.7E-23
score: 82.2
IPR029413Rhamnogalacturonan lyase, domain IICDDcd10316RGL4_Mcoord: 331..430
e-value: 3.9157E-33
score: 120.431
IPR014718Glycoside hydrolase-type carbohydrate-bindingGENE3D2.70.98.10coord: 7..304
e-value: 5.0E-13
score: 51.0
IPR011013Galactose mutarotase-like domain superfamilySUPERFAMILY74650Galactose mutarotase-likecoord: 12..296
IPR008979Galactose-binding-like domain superfamilySUPERFAMILY49785Galactose-binding domain-likecoord: 437..629
IPR013784Carbohydrate-binding-like foldSUPERFAMILY49452Starch-binding domain-likecoord: 328..423

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0037436.1Lag0037436.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0102210 rhamnogalacturonan endolyase activity
molecular_function GO:0003824 catalytic activity